Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0 0.05 0 0.06 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0.13 0.12 0.17 0.07 0.09 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.07 0 0 0 0.14 0.19 0.13 0.14 0.21 0.24 0.19 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.88 0.87 0.87 0.89 0.87 0.8 0.75 0.76 0.73 0.69 0.7 0.71 0.81 0.84 0.77 0.83 0.79 0.79 0.78 0.79 0.72 0.8 0.73 0.72
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.3 0.29 0.21 0.16 0.18 0.25 0.28 0.31 0.35 0.41 0.4 0.39 0.07 0.06 0.1 0.11 0.08 0.17 0.11 0.06 0.2 0.1 0.11 0.14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 0 4 2 3 2 6 4 3 0 0 0 0 0 0 1 0 2 2 3 2
Bud Neck 0 0 0 0 1 1 2 0 1 2 2 0 1 0 0 0 0 0 0 0 1 0 1 0
Bud Site 1 0 2 5 3 14 30 9 24 15 29 36 0 0 1 0 1 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 2 6 9 14 13 13 16 7 15 5 7 26 39 52 9 14 15 0 0 0 2 6 2
Endoplasmic Reticulum 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 4 0 2 0 0 0 0 0 0
Endosome 2 0 3 0 0 0 0 0 0 0 0 0 0 1 4 0 5 0 3 2 5 3 2 4
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 2 3 4 0 1 0
Mitochondria 30 5 4 4 6 68 120 82 64 120 114 100 0 0 0 5 6 7 1 1 2 2 2 5
Nucleus 187 66 320 234 249 388 462 469 345 390 336 378 158 279 238 106 118 128 171 72 292 268 214 214
Nuclear Periphery 1 0 6 6 0 16 13 5 10 6 8 12 0 4 1 0 3 1 0 1 0 2 0 2
Nucleolus 63 22 76 42 50 123 175 192 164 233 191 208 14 21 30 14 12 28 24 5 81 32 32 40
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 2 2 1 0 2 2 3 1 5 9 2 3 1 4 0 2 3 5 5
Vac/Vac Membrane 2 0 4 0 1 3 4 3 2 3 5 0 1 3 4 2 7 7 7 2 9 11 13 14
Unique Cell Count 213 76 369 263 285 487 616 615 470 567 478 532 194 333 311 127 150 162 220 92 406 335 293 300
Labelled Cell Count 288 95 423 300 325 634 823 781 620 792 696 747 201 352 340 144 171 189 220 92 406 335 293 300


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 7.8 7.2 6.5 6.2 5.5 5.2 5.3 5.2 5.1 4.9 5.0 8.3 7.9 7.7 8.7 8.8 8.3 8.2 8.5 8.9
Std Deviation (1e-4) 1.0 1.7 1.4 1.6 1.6 1.4 1.5 1.4 1.4 1.3 1.4 1.3 2.1 1.5 1.8 1.6 1.8 1.9 1.3 1.5 1.5
Intensity Change (Log2) – – – -0.14 -0.21 -0.39 -0.46 -0.43 -0.45 -0.49 -0.56 -0.52 0.22 0.15 0.1 0.28 0.29 0.21 0.19 0.25 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 2.5 3.7 0 3.8 2.4 0 4.6 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 2.4 1.0 0.5 1.0 -0.2 1.0 0 -0.4 5.7 5.4 7.0 0 4.1 4.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.7 8.4 6.6 6.6 8.9 0 8.2 0 0 0 0 0 0
Nucleus 0.8 0.2 -2.7 -4.4 -4.0 -4.7 -6.3 -5.7 -5.5 -1.7 -1.1 -3.5 -0.9 -2.3 -2.3
Nuclear Periphery 0 0 1.5 0.5 0 0.5 0 0 0.7 0 0 0 0 0 0
Nucleolus -1.5 -1.0 1.6 2.7 3.6 4.5 6.5 0 5.9 -4.1 -5.5 -3.9 -2.4 -3.5 -0.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – 0.032 0.0016 0.0139 0.007 0.0517 0.0011 0.0129 0 0.0006 0.0577 0.0063 0
Bud – – – – – – 0.0009 0.0002 0.0004 0.0026 0.0016 0.0002 0.0002 0 0.0001 0.0003 0.0002 0.0001
Bud Neck – – – – – – 0.0034 0.0002 0.001 0.0009 0.0021 0.0006 0.0013 0 0.0002 0.001 0.0009 0.001
Bud Periphery – – – – – – 0.0023 0.0004 0.0008 0.0043 0.0035 0.0002 0.0005 0 0.0002 0.0006 0.0007 0.0002
Bud Site – – – – – – 0.0043 0.0003 0.0044 0.0072 0.0053 0.0002 0.001 0 0.0002 0.0015 0.0006 0.0002
Cell Periphery – – – – – – 0.0008 0.0001 0.0004 0.0008 0.0011 0 0.0002 0 0.0001 0.0002 0.0002 0.0001
Cytoplasm – – – – – – 0.0035 0.0006 0.0008 0.0023 0.0036 0.0002 0.0039 0 0.0001 0.0008 0.0027 0
Cytoplasmic Foci – – – – – – 0.0528 0.0005 0.0182 0.0182 0.0207 0.001 0.0046 0 0.0015 0.0048 0.0032 0
Eisosomes – – – – – – 0.0004 0 0.0001 0.0002 0.0003 0 0.0002 0 0.0001 0.0007 0 0
Endoplasmic Reticulum – – – – – – 0.0045 0.0011 0.0063 0.0016 0.0035 0.0002 0.0029 0 0.0001 0.0052 0.0017 0
Endosome – – – – – – 0.0423 0.0013 0.0177 0.0243 0.0337 0.0067 0.009 0 0.0012 0.0231 0.0051 0.0002
Golgi – – – – – – 0.0091 0.0003 0.0083 0.0177 0.0141 0.003 0.0033 0 0.0004 0.0148 0.0013 0.0001
Lipid Particles – – – – – – 0.0271 0.0005 0.0135 0.0351 0.0422 0.0004 0.0081 0 0.0047 0.0211 0.0008 0.0005
Mitochondria – – – – – – 0.0186 0.0015 0.0188 0.0754 0.0129 0.0011 0.0062 0 0.0057 0.0074 0.004 0.0024
None – – – – – – 0.0028 0.001 0.0002 0.0011 0.0021 0 0.0027 0 0.0001 0.0022 0.0016 0
Nuclear Periphery – – – – – – 0.0168 0.0017 0.0017 0.0218 0.0138 0.0006 0.0215 0.0008 0.0008 0.0069 0.0057 0.0011
Nucleolus – – – – – – 0.05 0.0258 0.0341 0.0169 0.1947 0.1012 0.0511 0.0255 0.02 0.0259 0.1917 0.1445
Nucleus – – – – – – 0.655 0.9551 0.841 0.719 0.5594 0.8767 0.8527 0.9733 0.9558 0.8144 0.7412 0.8479
Peroxisomes – – – – – – 0.0234 0.0003 0.0076 0.0276 0.0117 0.0002 0.0012 0 0.0008 0.0038 0.0003 0
Punctate Nuclear – – – – – – 0.0275 0.0067 0.002 0.0055 0.0089 0.0058 0.0132 0.0002 0.0068 0.0052 0.0293 0.001
Vacuole – – – – – – 0.0163 0.0004 0.0064 0.006 0.0095 0.0003 0.0014 0 0.0004 0.001 0.0011 0.0004
Vacuole Periphery – – – – – – 0.0061 0.0003 0.0023 0.0044 0.004 0.0002 0.0018 0 0.0002 0.0013 0.0014 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.6342 10.7394 7.9691 14.4047 15.5762 5.5417 15.4527 18.0402 12.2125 14.985
Translational Efficiency 1.5782 1.6469 2.1804 0.9302 1.4252 4.5493 1.3034 1.1647 1.7825 1.6557

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Pgd1

Pgd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pgd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available