Standard name
Human Ortholog
Description Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 0.82 0.95 0.85 0.8 0.73 0.7 0.67 0.67 0.68 0.65 0.98 0.98 0.99 0.72 0.67 0.63 0.24 0.33 0.3 0.35 0.36 0.38
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0.09 0 0 0.05 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0 0 0 0 0.07 0.07 0.05 0.12 0.27 0.35 0 0 0 0 0 0 0.05 0 0.07 0 0 0
Nucleus 0 0 0 0.08 0 0.05 0.09 0.12 0.13 0.06 0 0 0 0 0.06 0.07 0.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Vac/Vac Membrane 0.08 0.17 0 0.15 0.21 0.17 0.21 0.17 0.15 0.16 0.15 0 0 0 0.28 0.36 0.34 0.51 0.45 0.41 0.43 0.43 0.37
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 0 0 2 2 0 0 4
Bud 0 0 0 0 1 0 4 3 2 2 0 0 0 1 1 0 1 1 9 1 1 8 1
Bud Neck 0 0 1 0 2 0 0 5 4 0 0 0 0 1 1 0 0 0 2 1 1 4 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 0 2 0 0 1
Cytoplasm 47 149 81 22 90 122 144 110 74 79 17 186 194 198 259 192 136 23 69 30 25 70 59
Endoplasmic Reticulum 0 2 0 0 1 2 0 2 3 0 0 1 2 2 17 16 10 0 0 1 1 2 4
Endosome 0 2 0 0 0 4 3 5 0 0 0 0 1 0 19 13 4 8 6 2 3 4 6
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 4 0 0 1 1
Mitochondria 10 2 0 0 0 11 14 8 13 31 9 0 2 1 5 1 2 5 6 7 3 6 5
Nucleus 2 3 2 2 3 9 18 19 14 7 1 0 0 0 21 19 22 0 1 0 1 1 0
Nuclear Periphery 0 0 0 0 0 0 1 0 1 0 1 0 2 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 1 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 2 0 1 0 0 0 0 0 0 1 4 1 4 5 3 1 6 2
Vac/Vac Membrane 5 30 3 4 24 29 44 28 17 18 4 2 0 0 100 102 74 50 95 40 31 84 57
Unique Cell Count 59 181 85 26 112 168 206 165 111 116 26 189 197 201 359 286 217 100 212 99 75 196 158
Labelled Cell Count 64 190 87 28 121 179 232 183 131 137 32 189 201 203 427 349 251 100 212 99 75 196 158


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.8 6.1 5.9 5.5 4.8 4.6 5.0 4.2 4.0 3.8 4.6 4.2 4.3 5.5 6.0 6.6 4.3 5.0 6.0
Std Deviation (1e-4) 0.4 1.2 1.9 4.0 1.8 1.9 1.3 1.7 1.0 1.2 0.5 1.5 1.5 1.8 1.6 1.6 2.2 1.3 2.1 2.5
Intensity Change (Log2) -0.05 -0.17 -0.36 -0.41 -0.28 -0.56 -0.61 -0.69 -0.42 -0.54 -0.5 -0.14 -0.03 0.11 -0.51 -0.3 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 4.8 5.8 5.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.8857 7.2661 16.1753 70.3168 93.0908 6.0928 9.1835 10.7436 30.5208 129.0647
Translational Efficiency 0.3086 0.6969 0.5334 0.8296 1.2271 1.4095 0.5833 0.619 1.3682 1.2784

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1396 1498 66 1273 2076 2233 1872 1300 3472 3731 1938 2573

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 670.65 689.33 857.10 801.18 719.63 698.41 820.16 810.32 699.94 694.76 821.42 805.80
Standard Deviation 87.31 103.19 111.02 123.91 79.44 104.22 101.18 114.76 86.11 103.90 101.75 119.46
Intensity Change Log 2 0.039635 0.353903 0.256566 -0.043181 0.188650 0.171236 -0.002638 0.270731 0.213029

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000190 0.000458 0.000136 0.002972 0.000308 0.000308 0.000117 0.001868 0.000261 0.000368 0.000117 0.002414
Bud Neck 0.112213 0.044655 0.001670 0.019614 0.043400 0.055017 0.005228 0.016405 0.071068 0.050857 0.005106 0.017993
Bud Site 0.036936 0.026648 0.000747 0.074907 0.014140 0.027624 0.012287 0.070833 0.023305 0.027232 0.011894 0.072848
Cell Periphery 0.002455 0.000145 0.000137 0.000316 0.000369 0.000139 0.000089 0.000170 0.001208 0.000142 0.000090 0.000242
Cytoplasm 0.316456* 0.378239* 0.172269* 0.422163* 0.416285* 0.361720* 0.536879* 0.465861* 0.376147* 0.368352* 0.524462* 0.444241*
Cytoplasmic Foci 0.059979 0.156081 0.024706 0.014829 0.156267 0.163418 0.012851 0.021597 0.117552 0.160472 0.013255 0.018249
Eisosomes 0.000203 0.000099 0.000081 0.000049 0.000276 0.000094 0.000024 0.000046 0.000247 0.000096 0.000026 0.000048
Endoplasmic Reticulum 0.002025 0.001282 0.003030 0.002749 0.001547 0.000754 0.000970 0.001689 0.001739 0.000966 0.001040 0.002213
Endosome 0.021352 0.033061 0.003501 0.014527 0.024320 0.047261 0.003823 0.011838 0.023126 0.041559 0.003813 0.013168
Golgi 0.001746 0.003950 0.000057 0.008469 0.002469 0.004815 0.000070 0.006820 0.002179 0.004468 0.000070 0.007636
Lipid Particles 0.003965 0.005946 0.003175 0.000942 0.007436 0.003762 0.001279 0.001231 0.006041 0.004639 0.001343 0.001088
Mitochondria 0.004096 0.006056 0.000267 0.008076 0.003556 0.008587 0.000683 0.003817 0.003773 0.007570 0.000669 0.005924
Mitotic Spindle 0.001438 0.003861 0.000036 0.119070* 0.002623 0.008296 0.015937 0.047608 0.002147 0.006516 0.015395 0.082964
None 0.006201 0.003776 0.003106 0.011320 0.002094 0.002076 0.007992 0.007847 0.003745 0.002759 0.007826 0.009565
Nuclear Periphery 0.000401 0.000544 0.001641 0.001491 0.000449 0.000345 0.001467 0.001244 0.000430 0.000425 0.001473 0.001366
Nuclear Periphery Foci 0.000314 0.000397 0.000791 0.001217 0.000242 0.000311 0.001254 0.000639 0.000271 0.000345 0.001239 0.000925
Nucleolus 0.001962 0.002505 0.002719 0.000906 0.001226 0.001638 0.000616 0.000735 0.001522 0.001986 0.000688 0.000820
Nucleus 0.106125 0.103529 0.720081* 0.211991* 0.122558 0.066238 0.332214* 0.227234* 0.115950 0.081210 0.345423* 0.219693*
Peroxisomes 0.003432 0.012994 0.000402 0.002999 0.004791 0.014000 0.000746 0.004382 0.004244 0.013596 0.000734 0.003698
Vacuole 0.315820* 0.212196* 0.061218 0.075438 0.193981 0.229776* 0.065175 0.106241 0.242969* 0.222718* 0.065040 0.091001
Vacuole Periphery 0.002692 0.003579 0.000229 0.005956 0.001663 0.003821 0.000299 0.001896 0.002077 0.003724 0.000296 0.003904

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.15 -0.29 -4.16 -2.84 -3.79 0.02 1.63 -1.94 -2.03 -2.55 -1.52 1.80 -3.89 -3.43 -4.62
Bud Neck 10.47 18.44 16.27 10.89 -9.61 -3.26 15.68 12.75 16.48 -5.10 6.24 22.60 20.07 19.60 -7.22
Bud Site 3.23 12.85 1.94 -0.90 -9.76 -6.40 3.76 -5.12 -1.35 -6.95 -1.83 8.96 -2.83 -1.56 -9.35
Cell Periphery 7.52 7.49 7.50 -0.08 0.08 5.37 6.95 5.87 1.77 -6.17 8.39 8.89 8.43 0.62 -4.49
Cytoplasm -5.71 5.22 -1.17 4.65 -5.68 6.39 -1.49 4.52 -0.92 5.53 1.09 -5.36 3.46 2.47 8.01
Cytoplasmic Foci -17.68 5.37 17.67 31.39 2.65 -1.48 37.92 36.35 36.37 -2.46 -10.82 38.57 39.02 48.31 0.07
Eisosomes 7.09 5.47 10.36 7.30 2.32 10.51 14.25 12.93 8.94 -7.37 12.64 18.08 16.31 11.52 -8.71
Endoplasmic Reticulum 2.48 -2.26 -5.12 -6.60 0.39 3.90 -1.42 -4.62 -12.82 -4.73 4.50 -1.07 -7.01 -12.42 -6.98
Endosome -5.22 9.98 11.37 15.61 -0.48 -10.09 17.38 13.57 19.99 -2.65 -11.28 20.61 17.98 25.28 -2.77
Golgi -2.24 2.18 -0.13 2.46 -3.00 -3.85 7.44 0.28 2.74 -2.81 -4.28 5.79 0.20 3.81 -4.05
Lipid Particles -2.26 0.38 4.59 7.41 2.30 7.60 13.79 13.84 9.53 -1.42 3.30 12.76 13.41 11.30 -0.96
Mitochondria -1.40 3.34 0.81 3.49 -5.41 -5.50 5.64 2.51 7.67 -2.68 -4.90 5.58 2.01 8.13 -5.21
Mitotic Spindle -2.12 2.07 -11.02 -10.51 -11.49 -3.66 -8.36 -8.67 -6.84 -2.13 -4.22 -8.68 -13.95 -12.24 -5.25
None 1.67 1.99 -1.54 -4.60 -4.75 0.07 -7.40 -7.49 -7.47 -0.30 1.57 -3.76 -5.68 -8.74 -1.55
Nuclear Periphery -1.96 -4.19 -17.09 -16.33 -5.26 1.73 -17.62 -19.04 -20.58 0.52 -0.02 -18.41 -24.59 -24.95 -2.43
Nuclear Periphery Foci -1.53 -2.11 -5.36 -4.75 -0.48 -1.12 -2.74 -7.94 -6.07 0.92 -1.75 -2.74 -8.66 -7.30 0.65
Nucleolus -1.12 -0.73 0.86 1.59 0.94 -2.56 0.30 3.74 5.13 1.61 -2.09 1.10 1.41 2.77 0.36
Nucleus 0.33 -16.22 -27.36 -27.33 9.73 13.92 -34.89 -21.90 -29.46 7.95 10.17 -38.38 -34.76 -40.97 8.08
Peroxisomes -7.57 4.52 3.40 10.74 -1.71 -8.84 10.81 5.20 11.33 -2.19 -11.63 10.13 6.58 15.68 -2.58
Vacuole 9.68 10.79 11.65 2.62 -5.22 -4.92 10.71 -1.73 2.32 -10.23 3.23 19.05 6.48 3.75 -11.88
Vacuole Periphery -1.48 7.69 2.38 3.42 -2.58 -8.50 12.84 3.69 9.44 -2.18 -6.12 12.79 3.57 7.91 -3.38
Standard name
Human Ortholog
Description Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p
Localization
Cell Percentages cytoplasm (15%), vacuole (10%), mixed (50%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Scw11

Scw11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Scw11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available