Standard name
Human Ortholog
Description Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; lacks a peroxisomal targeting signal but is imported into peroxisomes via binding to Gpd1p; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.31 0.25 0.27 0.35 0.27 0.25 0.24 – 0.3 0.24 0.23 0.31 0.23 0.15 0.15 0.06 0.13 0.1 0.28 0.24 0.2 0.26 0.46 0.47
Bud 0.05 0.09 0.09 0.11 0.07 0.11 0.05 – 0.06 0 0.1 0.08 0 0.05 0.06 0 0 0 0 0.05 0 0.06 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0.07 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0.06 0 0.08 0.16 0.14 0.28 – 0.14 0.29 0.2 0.18 0.17 0.22 0.17 0.42 0.31 0.42 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0.05 0.05 0.05 – 0.05 0.06 0.1 0.14 0.08 0.09 0.14 0 0 0 0 0 0 0 0 0
Endosome 0.26 0.1 0.12 0.16 0.05 0.18 0.06 – 0.09 0.07 0.05 0 0.07 0 0 0.09 0.12 0 0.17 0.1 0.06 0.11 0.07 0
Golgi 0.2 0.22 0.3 0.21 0.55 0.11 0.28 – 0.25 0.29 0.33 0.36 0.53 0.6 0.57 0.42 0.44 0.45 0.06 0 0.05 0.06 0.09 0.09
Mitochondria 0.06 0.21 0 0 0 0.24 0 – 0.12 0.05 0.08 0.07 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0.09 0.09 0.05 0 0.17 0.06 – 0.09 0.07 0.1 0.11 0 0 0.06 0 0.05 0 0 0 0 0 0 0
Peroxisomes 0.12 0.25 0.18 0.15 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.34 0.36 0.49 0.36 0.24 0.29
SpindlePole 0.23 0.1 0.22 0.24 0.12 0 0 – 0 0 0 0 0.07 0 0 0 0.1 0 0.07 0.13 0.14 0.09 0 0
Vac/Vac Membrane 0 0 0.05 0 0.05 0.12 0.16 – 0.14 0.05 0.09 0.07 0 0 0 0.08 0.15 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 47 35 37 43 45 28 48 – 31 39 37 66 44 43 36 5 15 10 53 50 33 44 151 135
Bud 8 12 13 14 11 12 10 – 6 6 16 17 7 15 13 3 1 2 2 11 5 9 8 9
Bud Neck 0 1 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 4 1 3 0 2
Bud Site 1 10 2 2 0 4 1 – 1 2 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 5 3 1 4 0 5 – 3 5 3 4 2 3 3 0 3 3 0 0 0 0 0 0
Cytoplasm 12 8 3 10 27 16 57 – 15 47 32 39 32 62 40 37 36 44 3 4 0 5 6 4
Endoplasmic Reticulum 3 2 1 0 8 6 11 – 5 10 16 29 16 25 32 2 4 2 0 0 0 0 2 3
Endosome 39 14 16 19 8 21 13 – 9 12 8 8 13 2 2 8 14 4 33 20 10 18 24 9
Golgi 30 30 42 26 92 12 56 – 26 47 52 76 102 172 135 37 51 47 11 7 8 9 29 24
Mitochondria 9 29 1 3 1 27 2 – 12 9 13 16 4 0 1 4 1 2 1 3 1 0 3 1
Nucleus 0 0 0 1 0 1 0 – 1 1 2 0 0 0 0 0 0 0 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 – 1 0 0 0 1 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 12 12 12 6 4 19 12 – 9 12 16 23 8 8 13 3 6 4 0 0 0 0 1 0
Peroxisomes 18 34 25 18 1 0 0 – 0 2 0 0 6 6 7 0 0 1 63 74 82 61 79 82
SpindlePole 35 14 31 29 20 5 9 – 1 3 3 4 13 7 5 3 12 4 13 26 23 15 13 7
Vac/Vac Membrane 5 3 7 5 8 14 32 – 15 8 15 16 3 5 3 7 17 12 0 0 0 0 0 2
Unique Cell Count 151 138 139 122 167 114 201 104 164 158 214 192 288 235 88 117 104 189 208 169 171 327 290
Labelled Cell Count 221 209 194 177 229 165 256 135 203 214 299 251 348 290 109 160 135 189 208 169 171 327 290


Peroxisomes

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.4 21.7 20.7 18.9 25.7 17.6 24.8 – 21.9 23.0 23.8 22.4 28.5 44.8 51.1 23.6 24.6 25.0 16.3 20.8 22.1
Std Deviation (1e-4) 4.4 7.9 5.0 5.6 7.3 7.6 15.4 – 7.7 9.5 9.5 7.8 11.0 18.1 19.7 10.2 6.8 8.6 11.7 13.6 7.4
Intensity Change (Log2) – – – -0.13 0.31 -0.24 0.26 – 0.08 0.15 0.2 0.11 0.46 1.11 1.3 0.19 0.25 0.27 -0.35 0.01 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.5 0.1 -0.4 -0.6 – 0.5 -0.6 -0.6 0.9 -0.8 -2.9 -2.7 -4.0 -2.7 -3.3
Bud 0.6 -0.9 0.3 -1.6 – -1.0 -2.0 0.2 -0.5 -2.2 -1.6 -1.4 -1.7 -3.0 -2.4
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.2 4.1 3.6 6.2 – 3.6 6.2 4.8 4.6 4.2 5.2 4.4 7.7 6.3 7.8
Endoplasmic Reticulum 0 0 0 0 – 0 2.5 3.5 4.2 3.1 3.2 4.2 0 0 0
Endosome 1.0 -2.2 1.5 -1.6 – -0.7 -1.3 -2.0 -2.8 -1.5 -5.2 -4.7 -0.6 0.1 -2.2
Golgi -1.6 4.4 -3.8 -0.5 – -0.9 -0.3 0.5 1.0 4.1 5.7 5.1 1.8 2.2 2.4
Mitochondria 0 0 5.8 0 – 3.7 0 3.0 2.9 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.2 -2.4 1.9 -0.9 – 0 -0.4 0.4 0.6 -1.7 -2.7 -1.2 -1.5 -1.1 -1.5
Peroxisomes -0.7 -5.4 -4.8 -6.2 – -4.6 -5.1 -5.6 -6.4 -4.6 -5.9 -5.0 -4.2 -4.8 -4.2
SpindlePole 0.3 -2.4 -4.1 -5.0 – -4.9 -5.6 -5.5 -6.3 -4.1 -6.8 -6.4 -3.9 -2.6 -4.1
Vacuole -0.4 -0.1 2.1 3.1 – 2.5 -0.1 1.5 0.9 0 0 0 0.9 2.6 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 21.3432 24.2179 22.6437 23.4316 18.5431 24.0923 21.2821 27.6074 25.0764 23.8243 25.1961 24.8172 28.5972 39.4032 29.9478 28.4963 26.6458 30.5899
Actin 0.1848 0.2252 0.2737 0.1604 0.0289 0.2765 0.1943 0.2823 0.2896 0.2755 0.1508 0.2692 0.2321 0.1651 0.2233 0.051 0.3043 0.3148
Bud 0.0006 0.0034 0.001 0.0008 0.0012 0.0003 0.0018 0.0026 0.0029 0.0039 0.0008 0.0007 0.0017 0.0046 0.0037 0.005 0.0004 0.0011
Bud Neck 0.0005 0.0011 0.0027 0.0051 0.0009 0.0016 0.0027 0.0009 0.002 0.0107 0.0009 0.0011 0.0011 0.001 0.0008 0.0017 0.0027 0.0018
Bud Periphery 0.0004 0.0016 0.0006 0.0005 0.0006 0.0002 0.0017 0.0011 0.0013 0.0033 0.0005 0.0006 0.001 0.0016 0.0025 0.0034 0.0002 0.0006
Bud Site 0.0018 0.0102 0.0036 0.0011 0.0015 0.0005 0.0132 0.023 0.0051 0.0114 0.0011 0.0008 0.0099 0.0315 0.0089 0.0153 0.0007 0.0017
Cell Periphery 0.0001 0.0011 0.0001 0.0008 0.0001 0.0001 0.0012 0.0001 0.0001 0.0025 0.0001 0.0001 0.0002 0.0002 0.0001 0.0007 0 0.0001
Cytoplasm 0.0108 0.0402 0.0044 0.0068 0.0076 0.0046 0.0256 0.0263 0.0311 0.0353 0.004 0.027 0.0089 0.0176 0.0242 0.0014 0.0591 0.0051
Cytoplasmic Foci 0.3417 0.3265 0.2996 0.4027 0.5378 0.3042 0.2768 0.3085 0.3075 0.2727 0.4149 0.3487 0.3226 0.3361 0.2692 0.3456 0.2097 0.3058
Eisosomes 0.0001 0.0002 0.0002 0.0002 0 0.0002 0.0008 0.0001 0.0002 0.0004 0.0002 0.0002 0.0006 0.0001 0.0001 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0003 0.0002 0.0006 0.0005 0.0003 0.0001 0.0056 0.0001 0.0004 0.0005 0.0001 0.003 0.0003 0.0001 0.0008 0.0001 0.0003 0.0001
Endosome 0.0183 0.0101 0.0102 0.03 0.0461 0.0078 0.0462 0.0089 0.0059 0.0251 0.0335 0.038 0.0116 0.02 0.0072 0.0093 0.018 0.0159
Golgi 0.0579 0.0336 0.0374 0.028 0.0231 0.0379 0.0506 0.025 0.0299 0.0199 0.0817 0.1017 0.0434 0.0247 0.0374 0.024 0.0568 0.0517
Lipid Particles 0.0584 0.0363 0.0309 0.03 0.0249 0.0548 0.053 0.0291 0.0132 0.0092 0.0726 0.0447 0.0659 0.0388 0.0126 0.0469 0.0339 0.0317
Mitochondria 0.0083 0.0084 0.0072 0.0032 0.0037 0.0037 0.0225 0.0093 0.0216 0.0314 0.0058 0.0076 0.0144 0.0088 0.0091 0.009 0.0019 0.004
None 0.0003 0.001 0.0015 0.0005 0.0022 0.0002 0.0118 0.0003 0.0014 0.0037 0.0002 0.0003 0.0054 0.0003 0.0065 0.0002 0.0003 0.0001
Nuclear Periphery 0.0009 0.0003 0.001 0.0015 0.0003 0.0001 0.0252 0.0001 0.0008 0.0011 0.0001 0.0035 0.0007 0.0007 0.0008 0.0001 0.0009 0.0003
Nucleolus 0.0146 0.0261 0.0003 0.0047 0.0018 0.0002 0.0011 0.0002 0.0003 0.0091 0.0002 0.0012 0.0017 0.0023 0.0013 0.0038 0.0001 0.0128
Nucleus 0.0042 0.0023 0.0003 0.0023 0.0009 0.0001 0.0123 0.0002 0.0044 0.003 0.0001 0.0003 0.0007 0.0123 0.0106 0.001 0.0013 0.0009
Peroxisomes 0.2895 0.2586 0.3206 0.3 0.3003 0.2987 0.2291 0.2748 0.2764 0.2232 0.2285 0.1456 0.2562 0.3214 0.3771 0.4779 0.3047 0.244
Punctate Nuclear 0.0054 0.011 0.0035 0.0105 0.0092 0.008 0.0196 0.0029 0.0049 0.0412 0.0032 0.004 0.0208 0.0115 0.0032 0.0016 0.0035 0.0068
Vacuole 0.0006 0.0022 0.0003 0.0058 0.0059 0.0002 0.0026 0.0035 0.0006 0.0153 0.0005 0.0006 0.0004 0.0009 0.0003 0.0011 0.0008 0.0004
Vacuole Periphery 0.0005 0.0007 0.0003 0.0046 0.0025 0.0002 0.0023 0.0009 0.0003 0.0018 0.0003 0.0011 0.0005 0.0003 0.0002 0.0006 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 76.2722 43.94 74.2053 196.1304 83.5073 83.006 91.9193 164.4042 214.2742 135.8879
Translational Efficiency 1.5939 1.2501 1.6255 1.0366 2.0179 2.7899 1.6639 1.4724 1.7993 1.7002

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
426 1515 153 1685 91 2706 1299 1479 517 4221 1452 3164

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2788.15 1190.30 3780.97 2280.15 1166.22 1240.77 1663.48 2445.33 2502.67 1222.66 1886.60 2357.36
Standard Deviation 877.10 276.32 859.27 771.45 250.95 249.98 479.69 1034.37 1013.17 260.87 840.44 907.67
Intensity Change Log 2 — -1.227983 0.439448 -0.290179 — 0.089396 0.512365 1.068189 — -0.701856 0.461338 0.257013

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.287376* 0.045084 0.148906* 0.335971* 0.104056* 0.026462 0.196023* 0.491855* 0.255109* 0.033146 0.191058* 0.408838*
Bud Neck 0.018331 0.006682 0.021259 0.006195 0.010570 0.002925 0.003666 0.005457 0.016965 0.004274 0.005520 0.005850
Bud Site 0.060636 0.071660 0.043896 0.032547 0.083899 0.039368 0.027714 0.036732 0.064731 0.050958 0.029419 0.034503
Cell Periphery 0.000354 0.000243 0.000338 0.000391 0.000360 0.000080 0.000144 0.000261 0.000355 0.000139 0.000165 0.000330
Cytoplasm 0.007084 0.030968 0.002723 0.009511 0.047860 0.031636 0.013761 0.008825 0.014261 0.031396 0.012598 0.009190
Cytoplasmic Foci 0.156697 0.349676* 0.309069* 0.086829 0.248036* 0.301753* 0.101282 0.065886 0.172774 0.318954* 0.123177 0.077039
Eisosomes 0.005347 0.000292 0.009630 0.003948 0.000304 0.000048 0.000743 0.001019 0.004459 0.000136 0.001680 0.002579
Endoplasmic Reticulum 0.000794 0.000332 0.000561 0.000234 0.001997 0.000082 0.000132 0.000294 0.001006 0.000171 0.000177 0.000262
Endosome 0.014465 0.029958 0.003781 0.009079 0.034447 0.031634 0.015045 0.025256 0.017982 0.031032 0.013858 0.016641
Golgi 0.035086 0.026292 0.018685 0.025219 0.045167 0.025199 0.017781 0.026581 0.036861 0.025591 0.017876 0.025855
Lipid Particles 0.067607 0.020084 0.052192 0.043233 0.039401 0.007412 0.022801 0.053618 0.062642 0.011961 0.025898 0.048087
Mitochondria 0.009491 0.001929 0.006092 0.006209 0.001081 0.001732 0.004075 0.004985 0.008010 0.001803 0.004288 0.005637
Mitotic Spindle 0.007733 0.017540 0.015971 0.011360 0.015608 0.041470 0.014747 0.013701 0.009119 0.032881 0.014876 0.012454
None 0.000190 0.000926 0.000152 0.000263 0.000194 0.000412 0.000085 0.000076 0.000191 0.000597 0.000092 0.000176
Nuclear Periphery 0.000211 0.000034 0.000529 0.000128 0.000064 0.000030 0.000072 0.000145 0.000185 0.000032 0.000120 0.000136
Nuclear Periphery Foci 0.001399 0.002854 0.000298 0.000446 0.004544 0.000771 0.000295 0.000292 0.001952 0.001518 0.000296 0.000374
Nucleolus 0.000536 0.000232 0.000272 0.000165 0.002077 0.000058 0.000240 0.000059 0.000807 0.000120 0.000243 0.000116
Nucleus 0.001821 0.000763 0.000127 0.000081 0.009141 0.000181 0.000042 0.000161 0.003109 0.000390 0.000051 0.000119
Peroxisomes 0.321508* 0.389977* 0.356620* 0.426418* 0.334211* 0.486464* 0.579968* 0.259809* 0.323744* 0.451833* 0.556433* 0.348537*
Vacuole 0.002927 0.003937 0.007122 0.001600 0.015950 0.002136 0.001281 0.004315 0.005219 0.002782 0.001897 0.002869
Vacuole Periphery 0.000409 0.000537 0.001777 0.000171 0.001035 0.000147 0.000101 0.000674 0.000519 0.000287 0.000278 0.000406

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 15.34* 5.62 -2.77 -31.87* -8.89 3.91 -4.60 -17.90 -46.08* -23.10* 16.23* 4.09 -10.10 -54.80* -21.80*
Bud Neck 5.76 -0.96 6.07 0.28 5.25 2.13 1.89 1.29 -7.17 -4.57 7.32 6.39 6.26 -4.99 -0.91
Bud Site -1.50 1.97 4.33 8.70 1.75 2.39 2.98 2.33 -0.24 -3.61 2.30 5.70 4.65 5.62 -2.58
Cell Periphery 2.97 -0.01 -0.39 -1.19 -0.35 4.17 3.08 0.85 -6.72 -4.20 7.80 6.04 -0.02 -2.87 -2.42
Cytoplasm -9.22 3.21 -1.54 8.82 -6.46 1.42 2.93 3.25 10.83 2.73 -5.73 0.91 2.01 13.91 2.29
Cytoplasmic Foci -15.23 -5.95 6.43 31.93 9.27 -2.20 5.98 7.40 38.87 5.87 -13.92 4.62 9.62 49.49 7.97
Eisosomes 6.92 -2.26 1.65 -8.66 3.17 3.23 -3.65 -5.31 -9.02 -1.87 7.12 4.34 2.89 -10.56 -2.91
Endoplasmic Reticulum 2.62 1.35 3.48 1.57 4.30 1.84 1.79 1.63 -9.91 -7.22 3.64 3.64 3.27 -3.24 -4.67
Endosome -5.97 5.84 2.91 9.98 -5.59 0.43 3.08 1.40 2.59 -4.92 -5.95 1.89 0.59 9.21 -1.98
Golgi 2.02 2.59 2.26 0.60 -1.31 2.92 4.01 2.63 -0.76 -4.22 3.05 5.01 2.90 -0.22 -4.89
Lipid Particles 6.36 1.31 3.09 -6.64 0.89 2.32 1.15 -1.19 -12.66 -7.51 7.83 5.39 1.97 -14.92 -7.19
Mitochondria 3.61 1.33 1.53 -5.86 -0.11 -1.07 -3.45 -3.99 -3.63 -0.80 3.60 2.02 1.33 -6.78 -1.62
Mitotic Spindle -4.88 -3.03 -2.15 2.62 1.39 -4.94 0.10 0.18 9.32 0.17 -11.55 -3.01 -2.31 10.12 1.02
None -2.09 1.67 -0.79 1.66 -1.10 -0.78 1.09 1.10 1.30 -0.01 -2.00 2.82 -0.13 1.84 -1.35
Nuclear Periphery 5.21 -2.01 2.43 -7.19 2.70 1.84 -0.41 -3.10 -4.76 -3.22 5.34 2.17 1.57 -7.49 -1.06
Nuclear Periphery Foci -1.37 1.31 1.11 3.23 -0.95 1.89 2.09 2.08 2.51 -0.35 0.70 2.27 2.16 3.89 -0.77
Nucleolus 1.17 1.13 1.26 0.94 1.17 1.12 1.02 1.12 -0.02 0.85 1.65 1.45 1.66 0.22 0.65
Nucleus 1.12 1.57 1.56 2.59 -0.10 1.14 1.16 1.13 -0.55 -1.21 1.77 1.96 1.87 1.30 -1.52
Peroxisomes -3.98 -1.10 -6.07 -3.13 -2.83 -4.78 -7.59 2.14 22.56 27.02* -8.72 -14.37 -1.69 13.17 19.79*
Vacuole -1.17 -2.12 2.72 3.64 2.86 2.22 2.34 1.86 -3.49 -4.72 2.12 2.69 1.77 -1.08 -2.86
Vacuole Periphery -0.52 -1.28 2.59 1.86 1.52 1.32 1.39 0.46 -2.26 -2.45 1.56 1.42 0.61 -0.99 -0.89
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; lacks a peroxisomal targeting signal but is imported into peroxisomes via binding to Gpd1p; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress
Localization
Cell Percentages cytoplasm (14%), actin (10%), mixed (39%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pnc1

Pnc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pnc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available