Standard name | |
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Human Ortholog | |
Description | Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; lacks a peroxisomal targeting signal but is imported into peroxisomes via binding to Gpd1p; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 47 | 35 | 37 | 43 | 45 | 28 | 48 | – | 31 | 39 | 37 | 66 | 44 | 43 | 36 | 5 | 15 | 10 | 53 | 50 | 33 | 44 | 151 | 135 |
Bud | 8 | 12 | 13 | 14 | 11 | 12 | 10 | – | 6 | 6 | 16 | 17 | 7 | 15 | 13 | 3 | 1 | 2 | 2 | 11 | 5 | 9 | 8 | 9 |
Bud Neck | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 3 | 0 | 2 |
Bud Site | 1 | 10 | 2 | 2 | 0 | 4 | 1 | – | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 5 | 3 | 1 | 4 | 0 | 5 | – | 3 | 5 | 3 | 4 | 2 | 3 | 3 | 0 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 12 | 8 | 3 | 10 | 27 | 16 | 57 | – | 15 | 47 | 32 | 39 | 32 | 62 | 40 | 37 | 36 | 44 | 3 | 4 | 0 | 5 | 6 | 4 |
Endoplasmic Reticulum | 3 | 2 | 1 | 0 | 8 | 6 | 11 | – | 5 | 10 | 16 | 29 | 16 | 25 | 32 | 2 | 4 | 2 | 0 | 0 | 0 | 0 | 2 | 3 |
Endosome | 39 | 14 | 16 | 19 | 8 | 21 | 13 | – | 9 | 12 | 8 | 8 | 13 | 2 | 2 | 8 | 14 | 4 | 33 | 20 | 10 | 18 | 24 | 9 |
Golgi | 30 | 30 | 42 | 26 | 92 | 12 | 56 | – | 26 | 47 | 52 | 76 | 102 | 172 | 135 | 37 | 51 | 47 | 11 | 7 | 8 | 9 | 29 | 24 |
Mitochondria | 9 | 29 | 1 | 3 | 1 | 27 | 2 | – | 12 | 9 | 13 | 16 | 4 | 0 | 1 | 4 | 1 | 2 | 1 | 3 | 1 | 0 | 3 | 1 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 1 | 0 | – | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 12 | 12 | 12 | 6 | 4 | 19 | 12 | – | 9 | 12 | 16 | 23 | 8 | 8 | 13 | 3 | 6 | 4 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 18 | 34 | 25 | 18 | 1 | 0 | 0 | – | 0 | 2 | 0 | 0 | 6 | 6 | 7 | 0 | 0 | 1 | 63 | 74 | 82 | 61 | 79 | 82 |
SpindlePole | 35 | 14 | 31 | 29 | 20 | 5 | 9 | – | 1 | 3 | 3 | 4 | 13 | 7 | 5 | 3 | 12 | 4 | 13 | 26 | 23 | 15 | 13 | 7 |
Vac/Vac Membrane | 5 | 3 | 7 | 5 | 8 | 14 | 32 | – | 15 | 8 | 15 | 16 | 3 | 5 | 3 | 7 | 17 | 12 | 0 | 0 | 0 | 0 | 0 | 2 |
Unique Cell Count | 151 | 138 | 139 | 122 | 167 | 114 | 201 | 104 | 164 | 158 | 214 | 192 | 288 | 235 | 88 | 117 | 104 | 189 | 208 | 169 | 171 | 327 | 290 | |
Labelled Cell Count | 221 | 209 | 194 | 177 | 229 | 165 | 256 | 135 | 203 | 214 | 299 | 251 | 348 | 290 | 109 | 160 | 135 | 189 | 208 | 169 | 171 | 327 | 290 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.4 | 21.7 | 20.7 | 18.9 | 25.7 | 17.6 | 24.8 | – | 21.9 | 23.0 | 23.8 | 22.4 | 28.5 | 44.8 | 51.1 | 23.6 | 24.6 | 25.0 | 16.3 | 20.8 | 22.1 |
Std Deviation (1e-4) | 4.4 | 7.9 | 5.0 | 5.6 | 7.3 | 7.6 | 15.4 | – | 7.7 | 9.5 | 9.5 | 7.8 | 11.0 | 18.1 | 19.7 | 10.2 | 6.8 | 8.6 | 11.7 | 13.6 | 7.4 |
Intensity Change (Log2) | – | – | – | -0.13 | 0.31 | -0.24 | 0.26 | – | 0.08 | 0.15 | 0.2 | 0.11 | 0.46 | 1.11 | 1.3 | 0.19 | 0.25 | 0.27 | -0.35 | 0.01 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.5 | 0.1 | -0.4 | -0.6 | – | 0.5 | -0.6 | -0.6 | 0.9 | -0.8 | -2.9 | -2.7 | -4.0 | -2.7 | -3.3 |
Bud | 0.6 | -0.9 | 0.3 | -1.6 | – | -1.0 | -2.0 | 0.2 | -0.5 | -2.2 | -1.6 | -1.4 | -1.7 | -3.0 | -2.4 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.2 | 4.1 | 3.6 | 6.2 | – | 3.6 | 6.2 | 4.8 | 4.6 | 4.2 | 5.2 | 4.4 | 7.7 | 6.3 | 7.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 2.5 | 3.5 | 4.2 | 3.1 | 3.2 | 4.2 | 0 | 0 | 0 |
Endosome | 1.0 | -2.2 | 1.5 | -1.6 | – | -0.7 | -1.3 | -2.0 | -2.8 | -1.5 | -5.2 | -4.7 | -0.6 | 0.1 | -2.2 |
Golgi | -1.6 | 4.4 | -3.8 | -0.5 | – | -0.9 | -0.3 | 0.5 | 1.0 | 4.1 | 5.7 | 5.1 | 1.8 | 2.2 | 2.4 |
Mitochondria | 0 | 0 | 5.8 | 0 | – | 3.7 | 0 | 3.0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -1.2 | -2.4 | 1.9 | -0.9 | – | 0 | -0.4 | 0.4 | 0.6 | -1.7 | -2.7 | -1.2 | -1.5 | -1.1 | -1.5 |
Peroxisomes | -0.7 | -5.4 | -4.8 | -6.2 | – | -4.6 | -5.1 | -5.6 | -6.4 | -4.6 | -5.9 | -5.0 | -4.2 | -4.8 | -4.2 |
SpindlePole | 0.3 | -2.4 | -4.1 | -5.0 | – | -4.9 | -5.6 | -5.5 | -6.3 | -4.1 | -6.8 | -6.4 | -3.9 | -2.6 | -4.1 |
Vacuole | -0.4 | -0.1 | 2.1 | 3.1 | – | 2.5 | -0.1 | 1.5 | 0.9 | 0 | 0 | 0 | 0.9 | 2.6 | 1.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 21.3432 | 24.2179 | 22.6437 | 23.4316 | 18.5431 | 24.0923 | 21.2821 | 27.6074 | 25.0764 | 23.8243 | 25.1961 | 24.8172 | 28.5972 | 39.4032 | 29.9478 | 28.4963 | 26.6458 | 30.5899 |
Actin | 0.1848 | 0.2252 | 0.2737 | 0.1604 | 0.0289 | 0.2765 | 0.1943 | 0.2823 | 0.2896 | 0.2755 | 0.1508 | 0.2692 | 0.2321 | 0.1651 | 0.2233 | 0.051 | 0.3043 | 0.3148 |
Bud | 0.0006 | 0.0034 | 0.001 | 0.0008 | 0.0012 | 0.0003 | 0.0018 | 0.0026 | 0.0029 | 0.0039 | 0.0008 | 0.0007 | 0.0017 | 0.0046 | 0.0037 | 0.005 | 0.0004 | 0.0011 |
Bud Neck | 0.0005 | 0.0011 | 0.0027 | 0.0051 | 0.0009 | 0.0016 | 0.0027 | 0.0009 | 0.002 | 0.0107 | 0.0009 | 0.0011 | 0.0011 | 0.001 | 0.0008 | 0.0017 | 0.0027 | 0.0018 |
Bud Periphery | 0.0004 | 0.0016 | 0.0006 | 0.0005 | 0.0006 | 0.0002 | 0.0017 | 0.0011 | 0.0013 | 0.0033 | 0.0005 | 0.0006 | 0.001 | 0.0016 | 0.0025 | 0.0034 | 0.0002 | 0.0006 |
Bud Site | 0.0018 | 0.0102 | 0.0036 | 0.0011 | 0.0015 | 0.0005 | 0.0132 | 0.023 | 0.0051 | 0.0114 | 0.0011 | 0.0008 | 0.0099 | 0.0315 | 0.0089 | 0.0153 | 0.0007 | 0.0017 |
Cell Periphery | 0.0001 | 0.0011 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0012 | 0.0001 | 0.0001 | 0.0025 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0007 | 0 | 0.0001 |
Cytoplasm | 0.0108 | 0.0402 | 0.0044 | 0.0068 | 0.0076 | 0.0046 | 0.0256 | 0.0263 | 0.0311 | 0.0353 | 0.004 | 0.027 | 0.0089 | 0.0176 | 0.0242 | 0.0014 | 0.0591 | 0.0051 |
Cytoplasmic Foci | 0.3417 | 0.3265 | 0.2996 | 0.4027 | 0.5378 | 0.3042 | 0.2768 | 0.3085 | 0.3075 | 0.2727 | 0.4149 | 0.3487 | 0.3226 | 0.3361 | 0.2692 | 0.3456 | 0.2097 | 0.3058 |
Eisosomes | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0008 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0003 | 0.0002 | 0.0006 | 0.0005 | 0.0003 | 0.0001 | 0.0056 | 0.0001 | 0.0004 | 0.0005 | 0.0001 | 0.003 | 0.0003 | 0.0001 | 0.0008 | 0.0001 | 0.0003 | 0.0001 |
Endosome | 0.0183 | 0.0101 | 0.0102 | 0.03 | 0.0461 | 0.0078 | 0.0462 | 0.0089 | 0.0059 | 0.0251 | 0.0335 | 0.038 | 0.0116 | 0.02 | 0.0072 | 0.0093 | 0.018 | 0.0159 |
Golgi | 0.0579 | 0.0336 | 0.0374 | 0.028 | 0.0231 | 0.0379 | 0.0506 | 0.025 | 0.0299 | 0.0199 | 0.0817 | 0.1017 | 0.0434 | 0.0247 | 0.0374 | 0.024 | 0.0568 | 0.0517 |
Lipid Particles | 0.0584 | 0.0363 | 0.0309 | 0.03 | 0.0249 | 0.0548 | 0.053 | 0.0291 | 0.0132 | 0.0092 | 0.0726 | 0.0447 | 0.0659 | 0.0388 | 0.0126 | 0.0469 | 0.0339 | 0.0317 |
Mitochondria | 0.0083 | 0.0084 | 0.0072 | 0.0032 | 0.0037 | 0.0037 | 0.0225 | 0.0093 | 0.0216 | 0.0314 | 0.0058 | 0.0076 | 0.0144 | 0.0088 | 0.0091 | 0.009 | 0.0019 | 0.004 |
None | 0.0003 | 0.001 | 0.0015 | 0.0005 | 0.0022 | 0.0002 | 0.0118 | 0.0003 | 0.0014 | 0.0037 | 0.0002 | 0.0003 | 0.0054 | 0.0003 | 0.0065 | 0.0002 | 0.0003 | 0.0001 |
Nuclear Periphery | 0.0009 | 0.0003 | 0.001 | 0.0015 | 0.0003 | 0.0001 | 0.0252 | 0.0001 | 0.0008 | 0.0011 | 0.0001 | 0.0035 | 0.0007 | 0.0007 | 0.0008 | 0.0001 | 0.0009 | 0.0003 |
Nucleolus | 0.0146 | 0.0261 | 0.0003 | 0.0047 | 0.0018 | 0.0002 | 0.0011 | 0.0002 | 0.0003 | 0.0091 | 0.0002 | 0.0012 | 0.0017 | 0.0023 | 0.0013 | 0.0038 | 0.0001 | 0.0128 |
Nucleus | 0.0042 | 0.0023 | 0.0003 | 0.0023 | 0.0009 | 0.0001 | 0.0123 | 0.0002 | 0.0044 | 0.003 | 0.0001 | 0.0003 | 0.0007 | 0.0123 | 0.0106 | 0.001 | 0.0013 | 0.0009 |
Peroxisomes | 0.2895 | 0.2586 | 0.3206 | 0.3 | 0.3003 | 0.2987 | 0.2291 | 0.2748 | 0.2764 | 0.2232 | 0.2285 | 0.1456 | 0.2562 | 0.3214 | 0.3771 | 0.4779 | 0.3047 | 0.244 |
Punctate Nuclear | 0.0054 | 0.011 | 0.0035 | 0.0105 | 0.0092 | 0.008 | 0.0196 | 0.0029 | 0.0049 | 0.0412 | 0.0032 | 0.004 | 0.0208 | 0.0115 | 0.0032 | 0.0016 | 0.0035 | 0.0068 |
Vacuole | 0.0006 | 0.0022 | 0.0003 | 0.0058 | 0.0059 | 0.0002 | 0.0026 | 0.0035 | 0.0006 | 0.0153 | 0.0005 | 0.0006 | 0.0004 | 0.0009 | 0.0003 | 0.0011 | 0.0008 | 0.0004 |
Vacuole Periphery | 0.0005 | 0.0007 | 0.0003 | 0.0046 | 0.0025 | 0.0002 | 0.0023 | 0.0009 | 0.0003 | 0.0018 | 0.0003 | 0.0011 | 0.0005 | 0.0003 | 0.0002 | 0.0006 | 0.0003 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 76.2722 | 43.94 | 74.2053 | 196.1304 | 83.5073 | 83.006 | 91.9193 | 164.4042 | 214.2742 | 135.8879 |
Translational Efficiency | 1.5939 | 1.2501 | 1.6255 | 1.0366 | 2.0179 | 2.7899 | 1.6639 | 1.4724 | 1.7993 | 1.7002 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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