Standard name
Human Ortholog
Description General transcription elongation factor TFIIS; enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 – 0 0 0.07 0 0 0 0 0 0 0 0.06 0.07 0.11 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0 0.05 0.05 0.11 0.09 0.08 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0.94 – 0.83 0.95 0.91 0.92 0.87 0.86 0.83 0.8 0.79 0.83 0.91 0.89 0.81 – – – 0.86 0.9 0.87 0.83 0.68 0.6
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.32 – 0.5 0.12 0.08 0.12 0.21 0.21 0.28 0.32 0.43 0.34 0.12 0.1 0.13 – – – 0.09 0 0.07 0.07 0.16 0.2
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0.11 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 – – – 0 0 0 0 0 0
Bud 0 – 0 0 0 0 1 1 0 0 0 2 0 0 0 – – – 1 0 0 1 4 4
Bud Neck 0 – 0 0 0 0 1 0 0 0 2 1 0 2 0 – – – 0 0 0 0 0 0
Bud Site 0 – 0 0 1 1 1 2 5 3 8 2 0 0 0 – – – – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 2 – 0 5 23 9 14 16 10 8 8 9 16 22 36 – – – 0 0 0 1 0 2
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 2 3
Endosome 0 – 0 0 2 0 0 0 0 0 0 0 1 0 2 – – – 0 0 0 0 0 2
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 1 1 1
Mitochondria 5 – 0 2 5 3 4 25 18 39 23 27 0 0 0 – – – 1 0 0 1 3 7
Nucleus 160 – 15 224 307 295 457 419 301 291 204 282 243 300 273 – – – 146 36 40 116 214 230
Nuclear Periphery 0 – 0 1 1 0 3 2 2 3 0 0 0 1 0 – – – 0 0 0 0 0 3
Nucleolus 54 – 9 28 28 40 112 101 101 118 112 115 32 34 45 – – – 14 0 3 9 50 78
Peroxisomes 0 – 0 0 0 0 0 0 1 0 0 0 0 0 1 – – – 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 1 1 0 0 1 0 1 0 5 8 – – – 0 0 0 2 1 1
Vac/Vac Membrane 1 – 0 0 2 3 5 1 4 2 3 2 2 2 6 – – – 3 0 0 4 33 42
Unique Cell Count 170 18 235 339 322 526 490 364 366 258 339 268 337 339 170 41 47 141 314 385
Labelled Cell Count 222 24 260 369 352 599 567 442 465 360 442 294 366 371 170 41 47 141 314 385


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.4 – 10.4 11.2 10.3 9.1 8.6 8.5 7.9 7.4 7.6 7.4 13.5 13.3 12.9 – – – 11.1 12.0 12.6
Std Deviation (1e-4) 2.4 – 1.8 2.5 4.1 1.9 2.1 2.3 1.8 1.7 1.9 1.8 4.8 3.6 4.0 – – – 2.8 2.9 2.7
Intensity Change (Log2) – – – 0.11 -0.01 -0.18 -0.27 -0.29 -0.39 -0.49 -0.45 -0.48 0.38 0.36 0.32 – – – 0.1 0.21 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleus 2.1 1.0 1.2 0.4 0.3 -0.1 -0.4 -0.4 0 1.0 0.7 -0.3 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 -2.0 -1.6 -0.5 -1.4 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.1609 11.4377 9.8099 8.7534 9.3391 9.474 11.1823 14.493 12.7893 12.6575 13.022 12.2846 14.0138 14.6362 12.7524 12.7642 12.0852 11.9981
Actin 0.0193 0 0.0006 0.0032 0.0008 0.0002 0.0663 0 0.0009 0.0001 0 0.0002 0.0075 0 0 0 0.0001 0.0029
Bud 0.001 0 0.0001 0.0028 0.0002 0.0001 0.0024 0.0001 0.0001 0 0.0001 0 0.0002 0 0 0 0.0001 0.0001
Bud Neck 0.0009 0 0.0001 0.0012 0.0002 0.0004 0.0069 0.0001 0.0001 0 0.0003 0.0004 0.0005 0 0 0 0.0004 0.0003
Bud Periphery 0.0014 0 0.0002 0.0058 0.0002 0.0002 0.0036 0.0002 0.0001 0 0.0002 0.0001 0.0003 0 0 0 0.0003 0.0002
Bud Site 0.0054 0 0.0002 0.0086 0.0006 0.0002 0.0199 0.0004 0.0002 0.0001 0.0001 0.0001 0.0007 0 0 0 0.0001 0.0001
Cell Periphery 0.0005 0 0.0001 0.0025 0.0003 0.0001 0.0011 0.0002 0 0.0001 0.0002 0.0001 0.0001 0 0 0 0.0003 0.0001
Cytoplasm 0.0234 0 0.0017 0.001 0.0029 0.0002 0.0036 0 0.0024 0.0003 0 0 0.001 0 0 0 0 0.0001
Cytoplasmic Foci 0.0547 0 0.0059 0.022 0.0175 0.0014 0.0289 0.0001 0.0035 0.0051 0 0.0006 0.009 0 0 0 0 0.0002
Eisosomes 0.0003 0 0 0 0.0001 0 0.0006 0 0 0 0 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0061 0 0.0008 0.0005 0.0017 0.0002 0.0012 0 0.0008 0.0001 0 0 0.0009 0 0 0 0 0.0001
Endosome 0.0738 0 0.0104 0.0181 0.0578 0.0063 0.0118 0.0003 0.0052 0.0007 0 0.0003 0.0055 0 0 0 0.0001 0.0008
Golgi 0.0319 0 0.0037 0.0052 0.0144 0.0022 0.0061 0.0001 0.002 0.0003 0 0.0007 0.0015 0 0 0 0 0.0003
Lipid Particles 0.0592 0 0.0041 0.0208 0.0289 0.0019 0.0098 0.0006 0.0052 0.0107 0 0.0064 0.0053 0 0 0 0.0001 0.0004
Mitochondria 0.0155 0.0002 0.009 0.0025 0.0108 0.0086 0.0075 0.006 0.0008 0.0023 0.0029 0.0015 0.0013 0.0002 0.0001 0.0002 0.0024 0.0004
None 0.0083 0 0.0002 0.0005 0.001 0.0001 0.0058 0 0.0006 0.0007 0 0 0.0037 0 0 0 0.0001 0.0004
Nuclear Periphery 0.0239 0.0016 0.002 0.0089 0.0042 0.0013 0.0037 0.0009 0.0007 0.002 0.0006 0.0004 0.0024 0.0004 0.0001 0.0004 0.0003 0.0015
Nucleolus 0.0734 0.0236 0.0229 0.0339 0.3433 0.1731 0.0723 0.017 0.0089 0.0322 0.3115 0.1389 0.0333 0.0253 0.0138 0.0124 0.3661 0.1204
Nucleus 0.5492 0.9738 0.9174 0.8359 0.501 0.8004 0.7137 0.9712 0.9631 0.9398 0.6831 0.8406 0.9172 0.9739 0.9859 0.9868 0.6271 0.8706
Peroxisomes 0.0182 0 0.0009 0.0032 0.0012 0.0001 0.0088 0.0002 0.0049 0.0004 0 0.0088 0.0018 0 0 0 0 0.0001
Punctate Nuclear 0.023 0.0003 0.0177 0.0127 0.0043 0.0013 0.0205 0 0.0003 0.0014 0.0002 0.0005 0.0068 0.0001 0 0.0002 0.0003 0.0007
Vacuole 0.0055 0.0002 0.0008 0.0081 0.0028 0.0007 0.0042 0.0016 0.0002 0.0009 0.0003 0.0002 0.0005 0 0 0 0.001 0.0002
Vacuole Periphery 0.0052 0.0003 0.0012 0.0025 0.0056 0.0009 0.0012 0.0008 0.0001 0.0027 0.0006 0.0002 0.0004 0 0 0 0.0011 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 46.2718 40.3035 30.694 54.5221 39.2265 34.3882 54.9601 54.6463 41.5595 56.8963
Translational Efficiency 2.2417 2.5409 2.8737 1.2289 2.5757 3.0116 1.6649 1.6648 2.2679 1.9234

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
227 314 2302 918 423 2436 1126 921 650 2750 3428 1839

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1205.87 1385.30 1479.80 1712.47 1076.35 1298.26 1474.75 1544.07 1121.58 1308.20 1478.14 1628.13
Standard Deviation 227.66 313.85 272.67 368.90 189.36 274.00 274.72 411.38 212.71 280.21 273.35 399.72
Intensity Change Log 2 — 0.200124 0.295328 0.506004 — 0.270432 0.454323 0.520591 — 0.233710 0.372501 0.512902

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000503 0.000480 0.000616 0.000557 0.000488 0.000557 0.000680 0.000713 0.000493 0.000548 0.000637 0.000635
Bud Neck 0.005580 0.004315 0.011353 0.006241 0.003912 0.009350 0.007750 0.010804 0.004494 0.008775 0.010170 0.008526
Bud Site 0.000394 0.000333 0.001387 0.000809 0.000367 0.001048 0.000718 0.001568 0.000376 0.000966 0.001168 0.001189
Cell Periphery 0.000014 0.000026 0.000133 0.000084 0.000061 0.000440 0.000042 0.000122 0.000045 0.000392 0.000103 0.000103
Cytoplasm 0.000030 0.000076 0.000072 0.000126 0.000247 0.000183 0.000066 0.003254 0.000171 0.000170 0.000070 0.001693
Cytoplasmic Foci 0.000015 0.000058 0.000179 0.000749 0.000039 0.000364 0.000124 0.002044 0.000030 0.000329 0.000161 0.001397
Eisosomes 0.000060 0.000071 0.000141 0.000074 0.000064 0.000146 0.000138 0.000063 0.000063 0.000137 0.000140 0.000068
Endoplasmic Reticulum 0.000931 0.000383 0.000492 0.000277 0.000850 0.001375 0.000641 0.000875 0.000879 0.001262 0.000541 0.000576
Endosome 0.000021 0.000009 0.000107 0.000039 0.000019 0.000359 0.000159 0.002843 0.000019 0.000319 0.000124 0.001444
Golgi 0.000035 0.000026 0.000081 0.000051 0.000033 0.000227 0.000120 0.001964 0.000034 0.000204 0.000094 0.001009
Lipid Particles 0.000008 0.000003 0.000089 0.000018 0.000006 0.000145 0.000391 0.001266 0.000007 0.000129 0.000188 0.000643
Mitochondria 0.000793 0.000911 0.001691 0.001712 0.000721 0.001664 0.001531 0.002688 0.000746 0.001578 0.001639 0.002200
Mitotic Spindle 0.000686 0.000203 0.000745 0.001171 0.000789 0.000983 0.000112 0.002433 0.000753 0.000894 0.000537 0.001803
None 0.000404 0.002442 0.000547 0.000658 0.000644 0.000700 0.000702 0.002777 0.000560 0.000899 0.000598 0.001719
Nuclear Periphery 0.000141 0.000037 0.000177 0.000084 0.000037 0.000214 0.000045 0.000420 0.000073 0.000194 0.000133 0.000252
Nuclear Periphery Foci 0.000029 0.000032 0.000068 0.000056 0.000020 0.000133 0.000052 0.001406 0.000023 0.000121 0.000063 0.000732
Nucleolus 0.042846 0.039767 0.030266 0.016963 0.046753 0.031145 0.037837 0.016782 0.045389 0.032129 0.032753 0.016873
Nucleus 0.945774* 0.948462* 0.949708* 0.968317* 0.942981* 0.948180* 0.945380* 0.942207* 0.943956* 0.948212* 0.948287* 0.955240*
Peroxisomes 0.001485 0.002119 0.001253 0.001274 0.001710 0.000774 0.002708 0.001784 0.001631 0.000928 0.001731 0.001530
Vacuole 0.000149 0.000131 0.000609 0.000366 0.000144 0.000841 0.000518 0.002519 0.000146 0.000760 0.000579 0.001444
Vacuole Periphery 0.000103 0.000115 0.000285 0.000373 0.000116 0.001173 0.000284 0.001468 0.000111 0.001052 0.000285 0.000921

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.92 -2.38 -1.09 -2.85 1.81 -1.38 -6.38 -6.63 -3.77 -1.99 -1.10 -5.53 -6.01 -2.75 -0.86
Bud Neck 1.16 -5.19 -0.81 -2.24 4.98 -9.10 -9.51 -5.89 -1.84 -2.93 -7.08 -9.56 -5.72 -0.59 1.17
Bud Site 0.54 -3.58 -1.66 -2.05 1.67 -4.46 -4.24 -4.83 -2.60 -3.73 -4.33 -4.39 -4.89 -1.87 -0.70
Cell Periphery -1.83 -2.61 -2.20 -1.83 0.93 -2.12 0.60 -1.30 1.79 -3.30 -2.23 -1.41 -1.76 1.86 -0.00
Cytoplasm -1.51 -2.02 -3.45 -1.06 -1.95 0.31 0.98 -2.26 -2.34 -2.45 -0.01 0.87 -2.25 -2.30 -2.49
Cytoplasmic Foci -2.57 -2.17 -1.48 -1.39 -1.15 -2.39 -1.64 -3.71 -2.98 -3.54 -2.49 -2.40 -3.62 -2.69 -3.22
Eisosomes -1.62 -8.85 -2.14 -0.53 7.14 -8.35 -12.10 -0.11 8.46 12.60 -8.71 -12.35 -1.67 7.89 11.20
Endoplasmic Reticulum 6.01 4.87 7.04 3.18 6.31 -5.57 3.11 -0.88 3.78 -3.04 -4.18 6.75 4.53 7.74 -1.21
Endosome 1.03 -1.89 -0.99 -1.68 1.45 -3.83 -1.03 -2.49 -2.13 -2.33 -3.79 -2.01 -2.50 -1.87 -2.27
Golgi 0.67 -1.22 -0.87 -1.05 0.39 -1.86 -1.28 -2.38 -2.08 -2.25 -1.85 -1.79 -2.42 -1.87 -2.23
Lipid Particles 2.15 -1.49 -0.81 -1.09 1.09 -2.30 -1.00 -1.65 -1.44 -1.01 -2.26 -1.43 -1.67 -1.31 -1.10
Mitochondria -0.77 -3.25 -5.20 -6.04 -0.02 -2.36 -3.26 -3.52 -1.48 -1.86 -2.32 -4.66 -5.05 -1.40 -1.56
Mitotic Spindle -0.29 -0.13 -0.44 -0.15 -0.41 -0.17 1.29 -2.12 -2.22 -3.66 -0.33 0.52 -1.64 -1.46 -2.27
None -1.58 -1.41 -3.09 1.39 -0.97 -0.30 -0.38 -2.77 -2.74 -2.73 -1.73 -0.33 -2.94 -2.02 -2.89
Nuclear Periphery 0.80 -0.36 0.62 -0.60 1.71 -3.67 -0.90 -3.29 -1.57 -3.22 -2.28 -1.28 -2.62 -0.69 -1.60
Nuclear Periphery Foci -0.84 -0.82 -0.61 0.61 -0.12 -1.86 -1.02 -2.17 -1.96 -2.11 -1.95 -1.45 -2.23 -1.79 -2.09
Nucleolus 0.30 2.33 5.05 5.36 6.01 3.78 2.01 5.79 4.17 5.53 3.98 3.69 7.83 7.17 7.64
Nucleus -0.10 -0.43 -3.65 -3.63 -5.15 -1.15 -0.52 0.87 2.05 1.47 -1.08 -0.97 -1.76 -1.03 -1.23
Peroxisomes -2.15 0.78 0.47 2.75 -0.26 4.49 -3.23 -0.50 -2.67 1.56 4.22 -0.76 -0.06 -2.90 0.46
Vacuole 1.42 -1.94 -1.20 -1.84 1.00 -4.69 -1.21 -2.58 -1.74 -2.04 -4.48 -2.43 -2.76 -1.35 -1.65
Vacuole Periphery -0.93 -3.86 -1.30 -1.24 -0.37 -3.49 -1.04 -2.52 -0.28 -2.07 -3.51 -2.91 -2.82 0.53 -2.09
Standard name
Human Ortholog
Description General transcription elongation factor TFIIS; enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Dst1

Dst1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dst1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available