Standard name | |
---|---|
Human Ortholog | |
Description | Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress; both human homologs ALG13 and ALG14 are required to complement yeast alg13 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 3 | 2 | 2 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 10 | 12 | 7 | 3 | 6 | 3 |
Bud | 0 | 4 | 0 | 2 | 0 | 3 | 0 | 6 | 4 | 4 | 7 | 7 | 1 | 0 | 0 | 1 | 3 | 0 | 5 | 3 | 0 | 1 | 3 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 10 | 2 | 2 | 5 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 6 | 11 | 8 | 10 | 5 | 13 | 14 | 10 | 6 | 8 | 19 | 6 | 15 | 23 | 15 | 20 | 5 | 3 | 9 | 6 | 2 | 7 | 3 |
Cytoplasm | 170 | 379 | 181 | 144 | 198 | 198 | 274 | 287 | 224 | 193 | 203 | 218 | 117 | 151 | 210 | 122 | 103 | 94 | 64 | 270 | 96 | 110 | 167 | 202 |
Endoplasmic Reticulum | 69 | 63 | 44 | 2 | 13 | 10 | 13 | 1 | 22 | 14 | 23 | 12 | 16 | 40 | 21 | 3 | 1 | 2 | 225 | 191 | 116 | 51 | 74 | 60 |
Endosome | 4 | 2 | 0 | 1 | 3 | 3 | 3 | 6 | 1 | 1 | 10 | 7 | 1 | 0 | 4 | 0 | 0 | 0 | 2 | 5 | 1 | 0 | 2 | 5 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 |
Mitochondria | 7 | 4 | 0 | 10 | 3 | 76 | 31 | 65 | 68 | 75 | 116 | 108 | 1 | 2 | 0 | 0 | 3 | 3 | 13 | 34 | 27 | 12 | 9 | 4 |
Nucleus | 0 | 1 | 0 | 0 | 3 | 5 | 8 | 5 | 6 | 8 | 8 | 10 | 1 | 0 | 2 | 7 | 8 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 3 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 3 |
Vac/Vac Membrane | 39 | 19 | 5 | 19 | 44 | 26 | 104 | 86 | 67 | 48 | 79 | 68 | 8 | 3 | 12 | 8 | 13 | 10 | 4 | 16 | 3 | 6 | 6 | 8 |
Unique Cell Count | 267 | 468 | 230 | 170 | 248 | 285 | 408 | 422 | 348 | 313 | 380 | 385 | 137 | 192 | 243 | 148 | 142 | 114 | 340 | 567 | 267 | 193 | 297 | 309 |
Labelled Cell Count | 292 | 484 | 243 | 189 | 276 | 329 | 449 | 474 | 404 | 352 | 457 | 452 | 151 | 213 | 272 | 157 | 151 | 124 | 340 | 567 | 267 | 193 | 297 | 309 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 5.4 | 6.4 | 6.3 | 7.3 | 5.3 | 6.6 | 6.4 | 5.8 | 5.8 | 5.8 | 5.7 | 6.6 | 6.5 | 7.6 | 8.9 | 9.2 | 8.7 | 5.3 | 6.2 | 6.2 |
Std Deviation (1e-4) | 1.0 | 0.8 | 0.9 | 1.3 | 1.1 | 1.5 | 1.2 | 1.3 | 1.2 | 1.2 | 1.1 | 1.2 | 1.2 | 1.5 | 5.7 | 1.5 | 1.8 | 1.8 | 1.6 | 1.7 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.18 | -0.28 | 0.05 | -0.0 | -0.15 | -0.15 | -0.14 | -0.16 | 0.05 | 0.02 | 0.24 | 0.48 | 0.53 | 0.45 | -0.26 | -0.05 | -0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | -0.4 | -2.0 | -1.0 | -0.9 | -1.2 | -1.9 | -1.8 | 0.1 | -0.2 | 1.3 | 2.0 | 2.0 | 3.2 | -0.2 |
Cytoplasm | 1.5 | 0.3 | -2.4 | -3.1 | -2.9 | -3.7 | -4.2 | -6.3 | -5.5 | 1.6 | 0 | 2.2 | 0.9 | -1.4 | 0.8 |
Endoplasmic Reticulum | -5.6 | -4.7 | -5.8 | -6.8 | -9.1 | -4.7 | -5.5 | -5.0 | -6.7 | -1.9 | 0.4 | -3.3 | -4.9 | -5.3 | -4.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 8.5 | 4.3 | 6.3 | 7.1 | 8.0 | 9.3 | 8.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.7 | 5.6 | 3.3 | 7.5 | 6.4 | 6.1 | 5.1 | 6.5 | 5.7 | 0 | 0 | 1.6 | 1.7 | 3.0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.141 | 1.4296 | 1.2705 | 1.1941 | 0.9423 | 1.415 | 2.4327 | 3.2839 | 2.9807 | 2.6611 | 3.0118 | 2.9874 | 4.2107 | 6.0301 | 4.6348 | 5.165 | 4.6816 | 5.3652 |
Actin | 0.0423 | 0.0024 | 0.0053 | 0.036 | 0.0007 | 0.0052 | 0.0027 | 0.0039 | 0.0057 | 0.0013 | 0.0036 | 0.0007 | 0.0003 | 0.0004 | 0.0016 | 0.0595 | 0.0018 | 0.0032 |
Bud | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0005 | 0.0004 | 0.0003 | 0.003 | 0.003 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0042 | 0.0009 | 0.0005 | 0.0018 | 0.0013 | 0.0047 | 0.0015 | 0.0007 | 0.0036 | 0.0005 | 0.0024 | 0.0059 | 0.0001 | 0.0002 | 0.0002 | 0.0009 | 0.0007 | 0.0039 |
Bud Periphery | 0.0008 | 0.0002 | 0.0001 | 0.0009 | 0.0001 | 0.0012 | 0.0003 | 0.0002 | 0.0012 | 0.0029 | 0.0002 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0004 |
Bud Site | 0.0043 | 0.0042 | 0.0006 | 0.0021 | 0 | 0.0004 | 0.0046 | 0.0112 | 0.0136 | 0.0011 | 0.0043 | 0.0002 | 0.0001 | 0.0016 | 0.0007 | 0.0002 | 0.0003 | 0.0001 |
Cell Periphery | 0.0011 | 0.0012 | 0.0006 | 0.001 | 0.0003 | 0.0004 | 0.0006 | 0.0007 | 0.0009 | 0.0006 | 0.0002 | 0.0002 | 0.0005 | 0.0015 | 0.0027 | 0.0012 | 0.0008 | 0.0004 |
Cytoplasm | 0.4284 | 0.6191 | 0.4624 | 0.592 | 0.7967 | 0.6191 | 0.532 | 0.674 | 0.5909 | 0.6806 | 0.6371 | 0.6687 | 0.5725 | 0.6793 | 0.4804 | 0.6095 | 0.6518 | 0.6564 |
Cytoplasmic Foci | 0.0167 | 0.0099 | 0.01 | 0.0063 | 0.0056 | 0.0268 | 0.0171 | 0.0153 | 0.0075 | 0.0099 | 0.0141 | 0.0092 | 0.0067 | 0.0032 | 0.0036 | 0.0206 | 0.0057 | 0.005 |
Eisosomes | 0.0023 | 0.0001 | 0.0004 | 0.0009 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.1338 | 0.1312 | 0.1826 | 0.213 | 0.1214 | 0.1312 | 0.2096 | 0.1477 | 0.1993 | 0.1328 | 0.0969 | 0.16 | 0.3318 | 0.1727 | 0.3745 | 0.1777 | 0.1367 | 0.199 |
Endosome | 0.017 | 0.0128 | 0.0097 | 0.0114 | 0.0099 | 0.0185 | 0.019 | 0.013 | 0.0114 | 0.0099 | 0.0248 | 0.0082 | 0.0082 | 0.0023 | 0.0039 | 0.0173 | 0.0044 | 0.0036 |
Golgi | 0.0058 | 0.0009 | 0.001 | 0.0059 | 0.0003 | 0.0023 | 0.0015 | 0.0016 | 0.0012 | 0.0006 | 0.0028 | 0.0002 | 0.0003 | 0.0002 | 0.0006 | 0.0053 | 0.0005 | 0.0002 |
Lipid Particles | 0.0077 | 0.0039 | 0.0028 | 0.0102 | 0.0006 | 0.0048 | 0.0039 | 0.0024 | 0.0018 | 0.0011 | 0.0158 | 0.0014 | 0.0046 | 0.0015 | 0.0029 | 0.0022 | 0.001 | 0.0006 |
Mitochondria | 0.0072 | 0.0003 | 0.0005 | 0.0133 | 0.0004 | 0.0009 | 0.0007 | 0.0005 | 0.0004 | 0.0006 | 0.0015 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.0003 | 0.0001 |
None | 0.2484 | 0.1912 | 0.3054 | 0.0862 | 0.0431 | 0.1661 | 0.1698 | 0.1028 | 0.1333 | 0.1369 | 0.1479 | 0.1108 | 0.0567 | 0.1253 | 0.1138 | 0.0896 | 0.1862 | 0.1186 |
Nuclear Periphery | 0.0343 | 0.0102 | 0.0095 | 0.0067 | 0.0116 | 0.0063 | 0.0162 | 0.0082 | 0.0095 | 0.0065 | 0.0106 | 0.0109 | 0.0107 | 0.0055 | 0.0085 | 0.007 | 0.0046 | 0.0038 |
Nucleolus | 0.0007 | 0.0002 | 0.0002 | 0.0004 | 0 | 0.0002 | 0.0006 | 0.0006 | 0.0005 | 0.0003 | 0.001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 |
Nucleus | 0.0258 | 0.0045 | 0.0033 | 0.0031 | 0.0038 | 0.0047 | 0.0096 | 0.0076 | 0.0087 | 0.006 | 0.02 | 0.0133 | 0.0039 | 0.0028 | 0.0024 | 0.0023 | 0.0025 | 0.0025 |
Peroxisomes | 0.0087 | 0.0001 | 0.0002 | 0.0016 | 0 | 0.0008 | 0.0007 | 0.0004 | 0.0002 | 0.0002 | 0.0028 | 0.0001 | 0 | 0 | 0.0001 | 0.001 | 0.0001 | 0 |
Punctate Nuclear | 0.0044 | 0.0016 | 0.0017 | 0.0012 | 0.0004 | 0.0027 | 0.0043 | 0.005 | 0.004 | 0.0022 | 0.0101 | 0.007 | 0.0006 | 0.0006 | 0.0004 | 0.0024 | 0.0008 | 0.0006 |
Vacuole | 0.0038 | 0.0044 | 0.0028 | 0.0047 | 0.0032 | 0.0028 | 0.0041 | 0.0035 | 0.0029 | 0.0026 | 0.0029 | 0.0021 | 0.0025 | 0.0025 | 0.003 | 0.0013 | 0.0012 | 0.0013 |
Vacuole Periphery | 0.0018 | 0.0004 | 0.0004 | 0.0008 | 0.0005 | 0.0004 | 0.0006 | 0.0003 | 0.0003 | 0.0003 | 0.0006 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.9301 | 1.6197 | 24.0372 | 24.9049 | 27.0148 | 25.3516 | 24.5646 | 19.8954 | 25.963 | 18.3983 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2243 | 1404 | 713 | 166 | 555 | 1821 | 1194 | 1860 | 2798 | 3225 | 1907 | 2026 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 724.26 | 725.45 | 932.35 | 979.49 | 694.37 | 814.42 | 937.77 | 997.33 | 718.33 | 775.69 | 935.74 | 995.87 |
Standard Deviation | 82.54 | 96.74 | 116.25 | 114.10 | 80.12 | 94.82 | 119.48 | 128.86 | 82.93 | 105.34 | 118.31 | 127.81 |
Intensity Change Log 2 | — | 0.002368 | 0.364364 | 0.435523 | — | 0.230068 | 0.433530 | 0.522366 | — | 0.118310 | 0.398632 | 0.478683 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000285 | 0.000510 | 0.000766 | 0.007542 | 0.000141 | 0.000343 | 0.000826 | 0.002898 | 0.000257 | 0.000416 | 0.000804 | 0.003279 |
Bud Neck | 0.011430 | 0.009975 | 0.009301 | 0.022925 | 0.003256 | 0.016441 | 0.007526 | 0.011070 | 0.009809 | 0.013626 | 0.008190 | 0.012042 |
Bud Site | 0.002988 | 0.009562 | 0.009775 | 0.044194 | 0.001765 | 0.007987 | 0.012328 | 0.032323 | 0.002746 | 0.008672 | 0.011374 | 0.033296 |
Cell Periphery | 0.000364 | 0.000344 | 0.000648 | 0.004096 | 0.000420 | 0.000330 | 0.000804 | 0.000830 | 0.000375 | 0.000336 | 0.000745 | 0.001098 |
Cytoplasm | 0.440935* | 0.433057* | 0.369621* | 0.241032* | 0.437584* | 0.474222* | 0.447952* | 0.467208* | 0.440271* | 0.456301* | 0.418665* | 0.448676* |
Cytoplasmic Foci | 0.179436 | 0.223132* | 0.032796 | 0.080707 | 0.166465 | 0.118246 | 0.056207 | 0.067847 | 0.176863 | 0.163908 | 0.047454 | 0.068901 |
Eisosomes | 0.000664 | 0.000281 | 0.000128 | 0.001577 | 0.000212 | 0.000319 | 0.000223 | 0.000405 | 0.000575 | 0.000303 | 0.000187 | 0.000501 |
Endoplasmic Reticulum | 0.027787 | 0.040451 | 0.038929 | 0.101513* | 0.015695 | 0.020442 | 0.055671 | 0.032628 | 0.025389 | 0.029153 | 0.049412 | 0.038272 |
Endosome | 0.008228 | 0.018401 | 0.006769 | 0.011980 | 0.008026 | 0.005753 | 0.006127 | 0.007602 | 0.008188 | 0.011259 | 0.006367 | 0.007961 |
Golgi | 0.006739 | 0.006896 | 0.000196 | 0.001672 | 0.001439 | 0.002074 | 0.000345 | 0.003711 | 0.005688 | 0.004173 | 0.000289 | 0.003544 |
Lipid Particles | 0.043644 | 0.029660 | 0.007459 | 0.040499 | 0.029198 | 0.017612 | 0.010346 | 0.013785 | 0.040779 | 0.022857 | 0.009267 | 0.015974 |
Mitochondria | 0.006825 | 0.005953 | 0.000515 | 0.003040 | 0.002730 | 0.004838 | 0.000313 | 0.001932 | 0.006013 | 0.005324 | 0.000388 | 0.002023 |
Mitotic Spindle | 0.001305 | 0.000899 | 0.001608 | 0.010174 | 0.000064 | 0.002188 | 0.003127 | 0.006978 | 0.001059 | 0.001627 | 0.002559 | 0.007240 |
None | 0.002511 | 0.005443 | 0.001684 | 0.000415 | 0.003787 | 0.002068 | 0.001290 | 0.001566 | 0.002764 | 0.003537 | 0.001437 | 0.001471 |
Nuclear Periphery | 0.002099 | 0.001866 | 0.006314 | 0.000848 | 0.002704 | 0.002107 | 0.004497 | 0.001218 | 0.002219 | 0.002002 | 0.005176 | 0.001187 |
Nuclear Periphery Foci | 0.001336 | 0.005720 | 0.021868 | 0.029536 | 0.005854 | 0.001654 | 0.025898 | 0.014228 | 0.002233 | 0.003424 | 0.024391 | 0.015482 |
Nucleolus | 0.000759 | 0.002254 | 0.000389 | 0.000186 | 0.001153 | 0.000892 | 0.000285 | 0.000577 | 0.000837 | 0.001485 | 0.000324 | 0.000545 |
Nucleus | 0.217698* | 0.131933* | 0.378805* | 0.058354 | 0.274254* | 0.284368* | 0.232974* | 0.112071 | 0.228916* | 0.218006* | 0.287498* | 0.107670 |
Peroxisomes | 0.004611 | 0.004644 | 0.000420 | 0.005304 | 0.001772 | 0.002738 | 0.000538 | 0.001902 | 0.004048 | 0.003568 | 0.000494 | 0.002181 |
Vacuole | 0.039365 | 0.066489 | 0.111783 | 0.330547* | 0.042894 | 0.034504 | 0.132562 | 0.218311* | 0.040065 | 0.048429 | 0.124793 | 0.227507* |
Vacuole Periphery | 0.000990 | 0.002531 | 0.000228 | 0.003859 | 0.000588 | 0.000875 | 0.000160 | 0.000910 | 0.000910 | 0.001596 | 0.000185 | 0.001152 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.59 | -5.89 | -4.56 | -4.39 | -4.13 | -2.15 | -7.26 | -8.18 | -7.39 | -5.66 | -2.14 | -7.53 | -8.84 | -8.33 | -6.84 |
Bud Neck | 1.30 | 0.72 | -2.29 | -2.67 | -2.48 | -8.71 | -6.44 | -9.18 | 3.55 | -2.85 | -3.41 | 0.86 | -1.90 | 1.74 | -2.73 |
Bud Site | -5.54 | -4.96 | -5.19 | -4.28 | -4.07 | -6.24 | -7.32 | -12.53 | -8.95 | -6.30 | -7.51 | -8.23 | -13.06 | -9.78 | -7.76 |
Cell Periphery | 0.92 | -5.93 | -2.81 | -2.83 | -2.52 | 2.71 | -5.69 | -5.87 | -8.79 | 0.88 | 2.24 | -8.88 | -5.75 | -6.16 | -1.89 |
Cytoplasm | 1.01 | 8.82 | 10.95 | 10.07 | 5.29 | -3.33 | 0.59 | -0.49 | 4.01 | -1.42 | -2.58 | 5.78 | 2.00 | 4.19 | -3.24 |
Cytoplasmic Foci | -6.98 | 35.29 | 13.32 | 17.26 | -6.75 | 6.33 | 15.66 | 14.13 | 13.30 | -4.01 | 2.91 | 36.33 | 29.32 | 26.72 | -8.44 |
Eisosomes | 7.17 | 10.57 | -2.84 | -4.54 | -5.19 | -3.82 | -0.19 | -4.28 | -1.01 | -3.94 | 6.35 | 9.43 | 2.57 | -3.52 | -6.85 |
Endoplasmic Reticulum | -3.50 | -2.78 | -4.39 | -3.37 | -3.62 | -1.75 | -8.51 | -3.88 | -2.56 | 5.85 | -1.64 | -7.01 | -2.44 | -1.01 | 4.23 |
Endosome | -8.27 | 2.37 | -2.36 | 4.57 | -3.57 | 2.83 | 2.65 | 1.35 | -2.16 | -1.91 | -4.59 | 4.25 | 1.67 | 5.87 | -2.23 |
Golgi | -0.15 | 12.43 | 9.10 | 9.28 | -4.36 | -2.23 | 4.81 | -1.55 | -0.80 | -2.97 | 3.08 | 12.69 | 3.53 | 1.92 | -3.14 |
Lipid Particles | 6.26 | 20.43 | 0.94 | -1.16 | -4.66 | 4.16 | 7.24 | 5.93 | 3.70 | -3.85 | 10.62 | 21.12 | 15.27 | 6.48 | -6.84 |
Mitochondria | 0.83 | 8.73 | 1.94 | 1.53 | -0.85 | -1.20 | 1.51 | 0.73 | 3.59 | -2.35 | 0.81 | 8.54 | 5.38 | 5.00 | -2.36 |
Mitotic Spindle | 0.55 | -1.23 | -1.39 | -1.46 | -1.09 | -3.01 | -3.64 | -5.26 | -3.04 | -1.63 | -0.95 | -2.70 | -4.38 | -3.94 | -2.19 |
None | -3.91 | 2.71 | 6.54 | 6.58 | 4.69 | 2.77 | 4.43 | 3.62 | 1.42 | -0.80 | -1.99 | 5.35 | 3.77 | 4.86 | -0.06 |
Nuclear Periphery | 1.04 | -8.73 | 6.63 | 5.12 | 11.74 | 1.45 | -3.51 | 3.28 | 3.02 | 8.57 | 1.15 | -9.59 | 4.45 | 3.53 | 12.73 |
Nuclear Periphery Foci | -7.63 | -9.44 | -7.11 | -5.78 | -1.51 | 4.62 | -9.75 | -6.11 | -14.10 | 5.77 | -3.51 | -16.09 | -14.69 | -13.02 | 5.30 |
Nucleolus | -3.40 | 6.55 | 11.23 | 4.67 | 4.49 | 1.23 | 6.78 | 3.01 | 1.29 | -2.22 | -2.96 | 10.63 | 1.99 | 3.66 | -1.87 |
Nucleus | 14.94 | -14.84 | 22.47 | 10.22 | 27.67 | -0.83 | 3.25 | 15.56 | 30.10 | 19.95 | 2.11 | -8.97 | 26.78 | 25.08 | 30.65 |
Peroxisomes | -0.18 | 13.25 | 0.02 | 0.10 | -3.92 | -3.93 | 9.17 | 0.11 | 3.05 | -4.85 | 1.48 | 13.95 | 5.83 | 5.24 | -6.04 |
Vacuole | -9.53 | -14.97 | -15.97 | -14.51 | -10.52 | 2.93 | -18.31 | -33.81 | -39.56 | -13.56 | -4.47 | -25.62 | -41.27 | -39.62 | -17.59 |
Vacuole Periphery | -6.74 | 7.11 | -0.72 | -0.18 | -0.98 | -1.42 | 5.78 | -0.48 | 0.61 | -2.48 | -4.25 | 9.12 | 0.07 | 2.08 | -2.29 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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