Standard name
Human Ortholog
Description Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress; both human homologs ALG13 and ALG14 are required to complement yeast alg13 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0.05 0 0 0 0 0 0 0 0.05 0 0.08 0.09 0.1 0.14 0 0 0 0 0 0 0
Cytoplasm 0.64 0.81 0.79 0.85 0.8 0.69 0.67 0.68 0.64 0.62 0.53 0.57 0.85 0.79 0.86 0.82 0.73 0.82 0.19 0.48 0.36 0.57 0.57 0.66
Endoplasmic Reticulum 0.26 0.13 0.19 0 0.05 0 0 0 0.06 0 0.06 0 0.12 0.21 0.09 0 0 0 0.66 0.34 0.44 0.27 0.25 0.19
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.27 0.08 0.15 0.2 0.24 0.31 0.28 0 0 0 0 0 0 0 0.06 0.1 0.07 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.08 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0 0 0.11 0.18 0.09 0.25 0.2 0.19 0.15 0.21 0.18 0.06 0 0.05 0.05 0.09 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 2 2 0 1 1 1 0 1 0 1 0 2 0 1 0 0 10 12 7 3 6 3
Bud 0 4 0 2 0 3 0 6 4 4 7 7 1 0 0 1 3 0 5 3 0 1 3 3
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 3 10 2 2 5 5
Bud Site 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 6 11 8 10 5 13 14 10 6 8 19 6 15 23 15 20 5 3 9 6 2 7 3
Cytoplasm 170 379 181 144 198 198 274 287 224 193 203 218 117 151 210 122 103 94 64 270 96 110 167 202
Endoplasmic Reticulum 69 63 44 2 13 10 13 1 22 14 23 12 16 40 21 3 1 2 225 191 116 51 74 60
Endosome 4 2 0 1 3 3 3 6 1 1 10 7 1 0 4 0 0 0 2 5 1 0 2 5
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 1
Mitochondria 7 4 0 10 3 76 31 65 68 75 116 108 1 2 0 0 3 3 13 34 27 12 9 4
Nucleus 0 1 0 0 3 5 8 5 6 8 8 10 1 0 2 7 8 9 0 0 0 0 0 0
Nuclear Periphery 0 3 0 0 1 0 1 1 2 2 2 1 0 0 0 0 0 1 0 1 0 0 1 0
Nucleolus 0 0 0 1 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 2
Peroxisomes 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 0 3
Vac/Vac Membrane 39 19 5 19 44 26 104 86 67 48 79 68 8 3 12 8 13 10 4 16 3 6 6 8
Unique Cell Count 267 468 230 170 248 285 408 422 348 313 380 385 137 192 243 148 142 114 340 567 267 193 297 309
Labelled Cell Count 292 484 243 189 276 329 449 474 404 352 457 452 151 213 272 157 151 124 340 567 267 193 297 309


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 5.4 6.4 6.3 7.3 5.3 6.6 6.4 5.8 5.8 5.8 5.7 6.6 6.5 7.6 8.9 9.2 8.7 5.3 6.2 6.2
Std Deviation (1e-4) 1.0 0.8 0.9 1.3 1.1 1.5 1.2 1.3 1.2 1.2 1.1 1.2 1.2 1.5 5.7 1.5 1.8 1.8 1.6 1.7 1.5
Intensity Change (Log2) – – – -0.03 0.18 -0.28 0.05 -0.0 -0.15 -0.15 -0.14 -0.16 0.05 0.02 0.24 0.48 0.53 0.45 -0.26 -0.05 -0.04

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.4 -2.0 -1.0 -0.9 -1.2 -1.9 -1.8 0.1 -0.2 1.3 2.0 2.0 3.2 -0.2
Cytoplasm 1.5 0.3 -2.4 -3.1 -2.9 -3.7 -4.2 -6.3 -5.5 1.6 0 2.2 0.9 -1.4 0.8
Endoplasmic Reticulum -5.6 -4.7 -5.8 -6.8 -9.1 -4.7 -5.5 -5.0 -6.7 -1.9 0.4 -3.3 -4.9 -5.3 -4.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 8.5 4.3 6.3 7.1 8.0 9.3 8.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.7 5.6 3.3 7.5 6.4 6.1 5.1 6.5 5.7 0 0 1.6 1.7 3.0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.141 1.4296 1.2705 1.1941 0.9423 1.415 2.4327 3.2839 2.9807 2.6611 3.0118 2.9874 4.2107 6.0301 4.6348 5.165 4.6816 5.3652
Actin 0.0423 0.0024 0.0053 0.036 0.0007 0.0052 0.0027 0.0039 0.0057 0.0013 0.0036 0.0007 0.0003 0.0004 0.0016 0.0595 0.0018 0.0032
Bud 0.0005 0.0001 0.0001 0.0004 0 0.0005 0.0004 0.0003 0.003 0.003 0.0003 0.0001 0 0 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0042 0.0009 0.0005 0.0018 0.0013 0.0047 0.0015 0.0007 0.0036 0.0005 0.0024 0.0059 0.0001 0.0002 0.0002 0.0009 0.0007 0.0039
Bud Periphery 0.0008 0.0002 0.0001 0.0009 0.0001 0.0012 0.0003 0.0002 0.0012 0.0029 0.0002 0.0001 0 0 0.0002 0.0001 0.0002 0.0004
Bud Site 0.0043 0.0042 0.0006 0.0021 0 0.0004 0.0046 0.0112 0.0136 0.0011 0.0043 0.0002 0.0001 0.0016 0.0007 0.0002 0.0003 0.0001
Cell Periphery 0.0011 0.0012 0.0006 0.001 0.0003 0.0004 0.0006 0.0007 0.0009 0.0006 0.0002 0.0002 0.0005 0.0015 0.0027 0.0012 0.0008 0.0004
Cytoplasm 0.4284 0.6191 0.4624 0.592 0.7967 0.6191 0.532 0.674 0.5909 0.6806 0.6371 0.6687 0.5725 0.6793 0.4804 0.6095 0.6518 0.6564
Cytoplasmic Foci 0.0167 0.0099 0.01 0.0063 0.0056 0.0268 0.0171 0.0153 0.0075 0.0099 0.0141 0.0092 0.0067 0.0032 0.0036 0.0206 0.0057 0.005
Eisosomes 0.0023 0.0001 0.0004 0.0009 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.1338 0.1312 0.1826 0.213 0.1214 0.1312 0.2096 0.1477 0.1993 0.1328 0.0969 0.16 0.3318 0.1727 0.3745 0.1777 0.1367 0.199
Endosome 0.017 0.0128 0.0097 0.0114 0.0099 0.0185 0.019 0.013 0.0114 0.0099 0.0248 0.0082 0.0082 0.0023 0.0039 0.0173 0.0044 0.0036
Golgi 0.0058 0.0009 0.001 0.0059 0.0003 0.0023 0.0015 0.0016 0.0012 0.0006 0.0028 0.0002 0.0003 0.0002 0.0006 0.0053 0.0005 0.0002
Lipid Particles 0.0077 0.0039 0.0028 0.0102 0.0006 0.0048 0.0039 0.0024 0.0018 0.0011 0.0158 0.0014 0.0046 0.0015 0.0029 0.0022 0.001 0.0006
Mitochondria 0.0072 0.0003 0.0005 0.0133 0.0004 0.0009 0.0007 0.0005 0.0004 0.0006 0.0015 0.0004 0.0001 0.0001 0.0002 0.0009 0.0003 0.0001
None 0.2484 0.1912 0.3054 0.0862 0.0431 0.1661 0.1698 0.1028 0.1333 0.1369 0.1479 0.1108 0.0567 0.1253 0.1138 0.0896 0.1862 0.1186
Nuclear Periphery 0.0343 0.0102 0.0095 0.0067 0.0116 0.0063 0.0162 0.0082 0.0095 0.0065 0.0106 0.0109 0.0107 0.0055 0.0085 0.007 0.0046 0.0038
Nucleolus 0.0007 0.0002 0.0002 0.0004 0 0.0002 0.0006 0.0006 0.0005 0.0003 0.001 0.0002 0 0.0001 0 0.0001 0.0001 0
Nucleus 0.0258 0.0045 0.0033 0.0031 0.0038 0.0047 0.0096 0.0076 0.0087 0.006 0.02 0.0133 0.0039 0.0028 0.0024 0.0023 0.0025 0.0025
Peroxisomes 0.0087 0.0001 0.0002 0.0016 0 0.0008 0.0007 0.0004 0.0002 0.0002 0.0028 0.0001 0 0 0.0001 0.001 0.0001 0
Punctate Nuclear 0.0044 0.0016 0.0017 0.0012 0.0004 0.0027 0.0043 0.005 0.004 0.0022 0.0101 0.007 0.0006 0.0006 0.0004 0.0024 0.0008 0.0006
Vacuole 0.0038 0.0044 0.0028 0.0047 0.0032 0.0028 0.0041 0.0035 0.0029 0.0026 0.0029 0.0021 0.0025 0.0025 0.003 0.0013 0.0012 0.0013
Vacuole Periphery 0.0018 0.0004 0.0004 0.0008 0.0005 0.0004 0.0006 0.0003 0.0003 0.0003 0.0006 0.0003 0.0002 0.0001 0.0002 0.0003 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.9301 1.6197 24.0372 24.9049 27.0148 25.3516 24.5646 19.8954 25.963 18.3983
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2243 1404 713 166 555 1821 1194 1860 2798 3225 1907 2026

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 724.26 725.45 932.35 979.49 694.37 814.42 937.77 997.33 718.33 775.69 935.74 995.87
Standard Deviation 82.54 96.74 116.25 114.10 80.12 94.82 119.48 128.86 82.93 105.34 118.31 127.81
Intensity Change Log 2 — 0.002368 0.364364 0.435523 — 0.230068 0.433530 0.522366 — 0.118310 0.398632 0.478683

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000285 0.000510 0.000766 0.007542 0.000141 0.000343 0.000826 0.002898 0.000257 0.000416 0.000804 0.003279
Bud Neck 0.011430 0.009975 0.009301 0.022925 0.003256 0.016441 0.007526 0.011070 0.009809 0.013626 0.008190 0.012042
Bud Site 0.002988 0.009562 0.009775 0.044194 0.001765 0.007987 0.012328 0.032323 0.002746 0.008672 0.011374 0.033296
Cell Periphery 0.000364 0.000344 0.000648 0.004096 0.000420 0.000330 0.000804 0.000830 0.000375 0.000336 0.000745 0.001098
Cytoplasm 0.440935 0.433057 0.369621 0.241032 0.437584 0.474222 0.447952 0.467208 0.440271 0.456301 0.418665 0.448676
Cytoplasmic Foci 0.179436 0.223132 0.032796 0.080707 0.166465 0.118246 0.056207 0.067847 0.176863 0.163908 0.047454 0.068901
Eisosomes 0.000664 0.000281 0.000128 0.001577 0.000212 0.000319 0.000223 0.000405 0.000575 0.000303 0.000187 0.000501
Endoplasmic Reticulum 0.027787 0.040451 0.038929 0.101513 0.015695 0.020442 0.055671 0.032628 0.025389 0.029153 0.049412 0.038272
Endosome 0.008228 0.018401 0.006769 0.011980 0.008026 0.005753 0.006127 0.007602 0.008188 0.011259 0.006367 0.007961
Golgi 0.006739 0.006896 0.000196 0.001672 0.001439 0.002074 0.000345 0.003711 0.005688 0.004173 0.000289 0.003544
Lipid Particles 0.043644 0.029660 0.007459 0.040499 0.029198 0.017612 0.010346 0.013785 0.040779 0.022857 0.009267 0.015974
Mitochondria 0.006825 0.005953 0.000515 0.003040 0.002730 0.004838 0.000313 0.001932 0.006013 0.005324 0.000388 0.002023
Mitotic Spindle 0.001305 0.000899 0.001608 0.010174 0.000064 0.002188 0.003127 0.006978 0.001059 0.001627 0.002559 0.007240
None 0.002511 0.005443 0.001684 0.000415 0.003787 0.002068 0.001290 0.001566 0.002764 0.003537 0.001437 0.001471
Nuclear Periphery 0.002099 0.001866 0.006314 0.000848 0.002704 0.002107 0.004497 0.001218 0.002219 0.002002 0.005176 0.001187
Nuclear Periphery Foci 0.001336 0.005720 0.021868 0.029536 0.005854 0.001654 0.025898 0.014228 0.002233 0.003424 0.024391 0.015482
Nucleolus 0.000759 0.002254 0.000389 0.000186 0.001153 0.000892 0.000285 0.000577 0.000837 0.001485 0.000324 0.000545
Nucleus 0.217698 0.131933 0.378805 0.058354 0.274254 0.284368 0.232974 0.112071 0.228916 0.218006 0.287498 0.107670
Peroxisomes 0.004611 0.004644 0.000420 0.005304 0.001772 0.002738 0.000538 0.001902 0.004048 0.003568 0.000494 0.002181
Vacuole 0.039365 0.066489 0.111783 0.330547 0.042894 0.034504 0.132562 0.218311 0.040065 0.048429 0.124793 0.227507
Vacuole Periphery 0.000990 0.002531 0.000228 0.003859 0.000588 0.000875 0.000160 0.000910 0.000910 0.001596 0.000185 0.001152

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.59 -5.89 -4.56 -4.39 -4.13 -2.15 -7.26 -8.18 -7.39 -5.66 -2.14 -7.53 -8.84 -8.33 -6.84
Bud Neck 1.30 0.72 -2.29 -2.67 -2.48 -8.71 -6.44 -9.18 3.55 -2.85 -3.41 0.86 -1.90 1.74 -2.73
Bud Site -5.54 -4.96 -5.19 -4.28 -4.07 -6.24 -7.32 -12.53 -8.95 -6.30 -7.51 -8.23 -13.06 -9.78 -7.76
Cell Periphery 0.92 -5.93 -2.81 -2.83 -2.52 2.71 -5.69 -5.87 -8.79 0.88 2.24 -8.88 -5.75 -6.16 -1.89
Cytoplasm 1.01 8.82 10.95 10.07 5.29 -3.33 0.59 -0.49 4.01 -1.42 -2.58 5.78 2.00 4.19 -3.24
Cytoplasmic Foci -6.98 35.29 13.32 17.26 -6.75 6.33 15.66 14.13 13.30 -4.01 2.91 36.33 29.32 26.72 -8.44
Eisosomes 7.17 10.57 -2.84 -4.54 -5.19 -3.82 -0.19 -4.28 -1.01 -3.94 6.35 9.43 2.57 -3.52 -6.85
Endoplasmic Reticulum -3.50 -2.78 -4.39 -3.37 -3.62 -1.75 -8.51 -3.88 -2.56 5.85 -1.64 -7.01 -2.44 -1.01 4.23
Endosome -8.27 2.37 -2.36 4.57 -3.57 2.83 2.65 1.35 -2.16 -1.91 -4.59 4.25 1.67 5.87 -2.23
Golgi -0.15 12.43 9.10 9.28 -4.36 -2.23 4.81 -1.55 -0.80 -2.97 3.08 12.69 3.53 1.92 -3.14
Lipid Particles 6.26 20.43 0.94 -1.16 -4.66 4.16 7.24 5.93 3.70 -3.85 10.62 21.12 15.27 6.48 -6.84
Mitochondria 0.83 8.73 1.94 1.53 -0.85 -1.20 1.51 0.73 3.59 -2.35 0.81 8.54 5.38 5.00 -2.36
Mitotic Spindle 0.55 -1.23 -1.39 -1.46 -1.09 -3.01 -3.64 -5.26 -3.04 -1.63 -0.95 -2.70 -4.38 -3.94 -2.19
None -3.91 2.71 6.54 6.58 4.69 2.77 4.43 3.62 1.42 -0.80 -1.99 5.35 3.77 4.86 -0.06
Nuclear Periphery 1.04 -8.73 6.63 5.12 11.74 1.45 -3.51 3.28 3.02 8.57 1.15 -9.59 4.45 3.53 12.73
Nuclear Periphery Foci -7.63 -9.44 -7.11 -5.78 -1.51 4.62 -9.75 -6.11 -14.10 5.77 -3.51 -16.09 -14.69 -13.02 5.30
Nucleolus -3.40 6.55 11.23 4.67 4.49 1.23 6.78 3.01 1.29 -2.22 -2.96 10.63 1.99 3.66 -1.87
Nucleus 14.94 -14.84 22.47 10.22 27.67 -0.83 3.25 15.56 30.10 19.95 2.11 -8.97 26.78 25.08 30.65
Peroxisomes -0.18 13.25 0.02 0.10 -3.92 -3.93 9.17 0.11 3.05 -4.85 1.48 13.95 5.83 5.24 -6.04
Vacuole -9.53 -14.97 -15.97 -14.51 -10.52 2.93 -18.31 -33.81 -39.56 -13.56 -4.47 -25.62 -41.27 -39.62 -17.59
Vacuole Periphery -6.74 7.11 -0.72 -0.18 -0.98 -1.42 5.78 -0.48 0.61 -2.48 -4.25 9.12 0.07 2.08 -2.29
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress; both human homologs ALG13 and ALG14 are required to complement yeast alg13 mutant
Localization
Cell Percentages ER (36%), cytoplasm (11%), mixed (42%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Alg13

Alg13


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Alg13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available