Standard name | |
---|---|
Human Ortholog | |
Description | Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | 0 | 0 | 4 | 8 | – | 7 | 11 | 4 | 5 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 3 | 1 | 0 | 0 | 0 | 0 | – | 1 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 31 | 11 | 5 | 8 | 8 | 7 | – | 10 | 16 | 7 | 4 | 6 | 15 | 17 | 7 | 15 | 6 | 0 | 0 | 0 | 1 | 0 | 0 |
Cytoplasm | 176 | 699 | 174 | 75 | 150 | 125 | 169 | – | 133 | 152 | 70 | 68 | 440 | 479 | 452 | 268 | 284 | 247 | 161 | 725 | 184 | 229 | 350 | 398 |
Endoplasmic Reticulum | 3 | 25 | 6 | 1 | 14 | 18 | 31 | – | 20 | 14 | 9 | 8 | 16 | 26 | 18 | 10 | 12 | 2 | 2 | 6 | 6 | 57 | 51 | 48 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 1 | 0 | 0 | 2 | 1 | – | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 1 | 0 | 2 |
Mitochondria | 1 | 2 | 2 | 1 | 0 | 6 | 10 | – | 49 | 61 | 45 | 49 | 2 | 0 | 0 | 1 | 3 | 2 | 1 | 2 | 4 | 3 | 7 | 4 |
Nucleus | 0 | 1 | 3 | 0 | 1 | 0 | 2 | – | 0 | 0 | 2 | 1 | 4 | 2 | 1 | 3 | 1 | 0 | 0 | 4 | 2 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 8 | 3 | 0 | 0 | 0 | 4 | – | 3 | 10 | 5 | 6 | 3 | 2 | 0 | 35 | 32 | 18 | 1 | 8 | 5 | 11 | 16 | 16 |
Unique Cell Count | 180 | 737 | 187 | 78 | 167 | 150 | 213 | 198 | 223 | 117 | 116 | 462 | 498 | 474 | 300 | 324 | 266 | 171 | 761 | 212 | 308 | 435 | 477 | |
Labelled Cell Count | 182 | 769 | 203 | 82 | 173 | 163 | 233 | 226 | 267 | 144 | 142 | 471 | 527 | 489 | 324 | 350 | 276 | 171 | 761 | 212 | 308 | 435 | 477 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.2 | 15.0 | 13.8 | 12.9 | 14.4 | 12.4 | 12.4 | – | 11.0 | 9.8 | 8.5 | 9.1 | 15.4 | 16.9 | 16.5 | 20.1 | 22.3 | 19.5 | 14.1 | 15.2 | 16.7 |
Std Deviation (1e-4) | 3.1 | 2.6 | 3.1 | 2.2 | 2.6 | 2.5 | 2.3 | – | 2.4 | 2.1 | 1.9 | 2.0 | 2.6 | 2.9 | 3.0 | 7.0 | 6.4 | 5.5 | 2.2 | 2.4 | 3.2 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.07 | -0.15 | -0.15 | – | -0.32 | -0.49 | -0.69 | -0.6 | 0.16 | 0.29 | 0.26 | 0.54 | 0.69 | 0.5 | 0.04 | 0.14 | 0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | -0.5 | -0.2 | -1.2 | – | -0.4 | 0.5 | 0 | -0.9 | 0 | -1.8 | -1.3 | -2.0 | -0.6 | -2.0 |
Cytoplasm | 1.0 | -1.1 | -2.8 | -3.9 | – | -6.3 | -6.2 | -7.1 | -7.3 | 1.1 | 1.7 | 1.2 | -1.4 | -1.9 | -0.1 |
Endoplasmic Reticulum | 0 | 2.1 | 3.1 | 3.9 | – | 2.7 | 1.4 | 1.8 | 1.5 | 0.2 | 1.1 | 0.4 | 0.1 | 0.3 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 2.1 | – | 6.8 | 7.4 | 8.8 | 9.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 4.0 | 3.6 | 2.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.5193 | 13.4217 | 12.2934 | 10.6116 | 10.8462 | 12.5133 | 9.9763 | 14.3782 | 12.5263 | 11.4736 | 11.3755 | 12.8232 | 11.6348 | 13.8797 | 12.6909 | 12.5237 | 12.0966 | 12.5598 |
Actin | 0.0159 | 0.0009 | 0.0143 | 0.0228 | 0.0092 | 0.0012 | 0.0166 | 0.001 | 0.017 | 0.0303 | 0.0048 | 0.0119 | 0.0038 | 0.003 | 0.0043 | 0.0073 | 0.002 | 0.0071 |
Bud | 0.0008 | 0.0004 | 0.0008 | 0.001 | 0.0008 | 0.0006 | 0.0012 | 0.0023 | 0.0034 | 0.0212 | 0.0009 | 0.0036 | 0.0037 | 0.0138 | 0.0071 | 0.0009 | 0.0024 | 0.0079 |
Bud Neck | 0.0008 | 0.0004 | 0.0006 | 0.0008 | 0.0014 | 0.0014 | 0.0016 | 0.0003 | 0.0013 | 0.0013 | 0.0005 | 0.0013 | 0.0009 | 0.0004 | 0.0009 | 0.0035 | 0.0013 | 0.0018 |
Bud Periphery | 0.0008 | 0.0002 | 0.0004 | 0.0005 | 0.0006 | 0.0004 | 0.0014 | 0.0007 | 0.0017 | 0.0048 | 0.0007 | 0.0021 | 0.0016 | 0.0044 | 0.0033 | 0.0009 | 0.0006 | 0.0037 |
Bud Site | 0.0014 | 0.001 | 0.0022 | 0.0008 | 0.0009 | 0.0002 | 0.0032 | 0.0063 | 0.0024 | 0.0017 | 0.0019 | 0.0004 | 0.0026 | 0.0164 | 0.0153 | 0.0017 | 0.0059 | 0.0012 |
Cell Periphery | 0.0019 | 0.0005 | 0.0008 | 0.0003 | 0.0003 | 0.0004 | 0.0017 | 0.0003 | 0.0006 | 0.0004 | 0.0001 | 0.0002 | 0.0005 | 0.0005 | 0.0007 | 0.0005 | 0.0002 | 0.0004 |
Cytoplasm | 0.7076 | 0.9447 | 0.8661 | 0.6852 | 0.7573 | 0.8093 | 0.6389 | 0.9221 | 0.8327 | 0.6173 | 0.7216 | 0.7953 | 0.8184 | 0.9143 | 0.8492 | 0.8139 | 0.8653 | 0.8528 |
Cytoplasmic Foci | 0.0201 | 0.006 | 0.0139 | 0.0199 | 0.0245 | 0.0194 | 0.0401 | 0.0159 | 0.0158 | 0.0249 | 0.0207 | 0.0195 | 0.0191 | 0.0105 | 0.0181 | 0.0172 | 0.0238 | 0.0218 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.1336 | 0.0229 | 0.0279 | 0.0796 | 0.025 | 0.0861 | 0.0711 | 0.0184 | 0.031 | 0.0232 | 0.0888 | 0.0472 | 0.0531 | 0.0068 | 0.0267 | 0.0324 | 0.0091 | 0.0347 |
Endosome | 0.053 | 0.0124 | 0.0416 | 0.1035 | 0.11 | 0.0458 | 0.1079 | 0.0187 | 0.0368 | 0.1015 | 0.0792 | 0.0733 | 0.0435 | 0.0154 | 0.0389 | 0.0503 | 0.0485 | 0.0395 |
Golgi | 0.0088 | 0.0019 | 0.0077 | 0.0055 | 0.0122 | 0.0068 | 0.0165 | 0.002 | 0.0059 | 0.0225 | 0.0117 | 0.0108 | 0.004 | 0.0013 | 0.0036 | 0.0054 | 0.0117 | 0.0033 |
Lipid Particles | 0.0046 | 0.0001 | 0.0007 | 0.0029 | 0.0041 | 0.0001 | 0.0105 | 0.0002 | 0.0099 | 0.0174 | 0.0026 | 0.0005 | 0.0009 | 0.0001 | 0.0004 | 0.0007 | 0.0036 | 0.0004 |
Mitochondria | 0.0071 | 0.0008 | 0.0021 | 0.0033 | 0.0089 | 0.0142 | 0.0156 | 0.0009 | 0.0066 | 0.0491 | 0.0018 | 0.0069 | 0.0036 | 0.0007 | 0.0114 | 0.0087 | 0.0032 | 0.0022 |
None | 0.0035 | 0.0005 | 0.0009 | 0.0028 | 0.0086 | 0.003 | 0.0038 | 0.0014 | 0.0044 | 0.0045 | 0.0014 | 0.0012 | 0.0057 | 0.0026 | 0.0034 | 0.0013 | 0.0015 | 0.0026 |
Nuclear Periphery | 0.0091 | 0.0013 | 0.0021 | 0.0194 | 0.01 | 0.002 | 0.015 | 0.001 | 0.0045 | 0.0052 | 0.0055 | 0.0101 | 0.0062 | 0.0008 | 0.0022 | 0.0072 | 0.0015 | 0.0029 |
Nucleolus | 0.0001 | 0 | 0 | 0.0003 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0011 | 0.001 | 0.0006 | 0.0001 | 0.001 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0001 |
Nucleus | 0.0031 | 0.001 | 0.0013 | 0.0033 | 0.0028 | 0.0012 | 0.0049 | 0.0011 | 0.0023 | 0.0034 | 0.003 | 0.0019 | 0.0068 | 0.0018 | 0.0032 | 0.0035 | 0.0026 | 0.0031 |
Peroxisomes | 0.0045 | 0 | 0.0004 | 0.0026 | 0.0016 | 0.0001 | 0.0088 | 0.0002 | 0.002 | 0.0151 | 0.0043 | 0.0008 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0005 | 0.0003 |
Punctate Nuclear | 0.0043 | 0.0001 | 0.0002 | 0.0103 | 0.0073 | 0.0004 | 0.0089 | 0.0005 | 0.0021 | 0.0007 | 0.037 | 0.0003 | 0.0013 | 0.0003 | 0.0007 | 0.0005 | 0.0014 | 0.0008 |
Vacuole | 0.0154 | 0.004 | 0.014 | 0.0264 | 0.0095 | 0.0049 | 0.0263 | 0.0055 | 0.0155 | 0.0408 | 0.0096 | 0.0078 | 0.0194 | 0.0058 | 0.0082 | 0.0369 | 0.0125 | 0.0111 |
Vacuole Periphery | 0.0033 | 0.0008 | 0.0021 | 0.0085 | 0.0043 | 0.0024 | 0.0055 | 0.0011 | 0.0028 | 0.0133 | 0.0032 | 0.0047 | 0.0037 | 0.0008 | 0.0019 | 0.0066 | 0.0023 | 0.0024 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 35.9762 | 31.9809 | 47.1957 | 42.6679 | 32.052 | 40.9781 | 41.8731 | 39.9707 | 41.7288 | 36.7575 |
Translational Efficiency | 2.1554 | 2.7113 | 1.6816 | 1.9259 | 2.2355 | 1.5504 | 1.7846 | 1.8752 | 1.7049 | 1.802 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1971 | 1551 | 226 | 1044 | 1118 | 1922 | 1863 | 565 | 3089 | 3473 | 2089 | 1609 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1243.50 | 1412.96 | 2471.05 | 1543.26 | 1162.77 | 1296.98 | 1751.37 | 1752.56 | 1214.28 | 1348.78 | 1829.23 | 1616.76 |
Standard Deviation | 182.28 | 243.15 | 334.12 | 280.81 | 174.87 | 644.18 | 262.92 | 369.29 | 183.78 | 509.29 | 351.70 | 330.20 |
Intensity Change Log 2 | — | 0.184314 | 0.990718 | 0.311575 | — | 0.157590 | 0.590918 | 0.591898 | — | 0.171462 | 0.811272 | 0.453839 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000111 | 0.000266 | 0.000587 | 0.001067 | 0.000221 | 0.000175 | 0.000163 | 0.000956 | 0.000151 | 0.000216 | 0.000209 | 0.001028 |
Bud Neck | 0.000179 | 0.000303 | 0.009860 | 0.001958 | 0.000298 | 0.000386 | 0.000851 | 0.001679 | 0.000222 | 0.000349 | 0.001825 | 0.001860 |
Bud Site | 0.015218 | 0.021542 | 0.026998 | 0.027964 | 0.021899 | 0.014380 | 0.010723 | 0.062513 | 0.017636 | 0.017578 | 0.012483 | 0.040096 |
Cell Periphery | 0.000300 | 0.000538 | 0.015065 | 0.003317 | 0.000513 | 0.000164 | 0.000639 | 0.008915 | 0.000377 | 0.000331 | 0.002200 | 0.005283 |
Cytoplasm | 0.787500* | 0.812209* | 0.516133* | 0.680720* | 0.756985* | 0.780886* | 0.873646* | 0.726123* | 0.776456* | 0.794874* | 0.834968* | 0.696663* |
Cytoplasmic Foci | 0.125824 | 0.091994 | 0.041446 | 0.031324 | 0.136428 | 0.109766 | 0.021256 | 0.042226 | 0.129662 | 0.101829 | 0.023441 | 0.035152 |
Eisosomes | 0.000002 | 0.000003 | 0.000008 | 0.000008 | 0.000003 | 0.000004 | 0.000002 | 0.000006 | 0.000003 | 0.000003 | 0.000002 | 0.000007 |
Endoplasmic Reticulum | 0.010601 | 0.005010 | 0.001474 | 0.001641 | 0.009216 | 0.003332 | 0.001734 | 0.002248 | 0.010100 | 0.004081 | 0.001706 | 0.001854 |
Endosome | 0.003713 | 0.004303 | 0.008920 | 0.012410 | 0.006828 | 0.008342 | 0.003294 | 0.006165 | 0.004841 | 0.006538 | 0.003902 | 0.010217 |
Golgi | 0.017467 | 0.022001 | 0.089405 | 0.016997 | 0.020144 | 0.017252 | 0.011119 | 0.045184 | 0.018436 | 0.019373 | 0.019589 | 0.026894 |
Lipid Particles | 0.000572 | 0.001224 | 0.019591 | 0.009422 | 0.000881 | 0.000660 | 0.003201 | 0.017112 | 0.000684 | 0.000912 | 0.004975 | 0.012122 |
Mitochondria | 0.000749 | 0.000726 | 0.000152 | 0.001183 | 0.000291 | 0.001256 | 0.000091 | 0.000077 | 0.000583 | 0.001020 | 0.000098 | 0.000795 |
Mitotic Spindle | 0.000671 | 0.002658 | 0.018608 | 0.004568 | 0.001375 | 0.002449 | 0.004104 | 0.002042 | 0.000925 | 0.002542 | 0.005673 | 0.003681 |
None | 0.000365 | 0.000234 | 0.000760 | 0.000897 | 0.000307 | 0.001185 | 0.000203 | 0.000201 | 0.000344 | 0.000760 | 0.000264 | 0.000653 |
Nuclear Periphery | 0.000045 | 0.000030 | 0.001066 | 0.000143 | 0.000117 | 0.000075 | 0.000112 | 0.000052 | 0.000071 | 0.000055 | 0.000215 | 0.000111 |
Nuclear Periphery Foci | 0.000724 | 0.000855 | 0.022557 | 0.002371 | 0.000946 | 0.000517 | 0.003376 | 0.003954 | 0.000804 | 0.000668 | 0.005451 | 0.002927 |
Nucleolus | 0.000030 | 0.000062 | 0.000133 | 0.000204 | 0.000024 | 0.000201 | 0.000052 | 0.000052 | 0.000028 | 0.000139 | 0.000061 | 0.000151 |
Nucleus | 0.001101 | 0.001014 | 0.009494 | 0.004607 | 0.001813 | 0.003443 | 0.003519 | 0.002287 | 0.001359 | 0.002358 | 0.004166 | 0.003792 |
Peroxisomes | 0.000117 | 0.000197 | 0.000097 | 0.000994 | 0.000174 | 0.000334 | 0.000123 | 0.000443 | 0.000137 | 0.000273 | 0.000121 | 0.000801 |
Vacuole | 0.009116 | 0.016194 | 0.160544 | 0.175625 | 0.017323 | 0.033522 | 0.051899 | 0.059731 | 0.012086 | 0.025784 | 0.063653 | 0.134929 |
Vacuole Periphery | 0.025594 | 0.018637 | 0.057103 | 0.022580 | 0.024216 | 0.021673 | 0.009893 | 0.018032 | 0.025095 | 0.020317 | 0.015000 | 0.020983 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.60 | -7.10 | -2.01 | -1.64 | -0.79 | 1.34 | 1.75 | -3.34 | -3.51 | -3.53 | -2.75 | -4.33 | -2.90 | -2.66 | -2.64 |
Bud Neck | -3.30 | -8.38 | -14.42 | -13.23 | 6.86 | -1.09 | -7.81 | -8.41 | -6.93 | -4.27 | -2.42 | -10.59 | -16.62 | -13.80 | 0.28 |
Bud Site | -4.78 | -4.55 | -5.39 | -2.33 | 0.27 | 4.93 | 7.29 | -7.09 | -8.51 | -9.07 | 0.04 | 4.85 | -8.74 | -8.79 | -10.58 |
Cell Periphery | -5.43 | -6.89 | -4.95 | -4.49 | 5.45 | 3.71 | -1.13 | -6.03 | -6.31 | -5.95 | 1.14 | -7.06 | -7.74 | -7.82 | -4.35 |
Cytoplasm | -3.03 | 12.15 | 10.32 | 12.50 | -7.08 | -2.36 | -12.39 | 2.27 | 4.21 | 11.47 | -2.89 | -7.40 | 9.69 | 12.07 | 15.06 |
Cytoplasmic Foci | 6.57 | 14.93 | 22.39 | 16.06 | 3.47 | 4.34 | 22.29 | 16.28 | 15.05 | -6.46 | 7.07 | 32.77 | 27.71 | 23.27 | -5.65 |
Eisosomes | -2.14 | -5.06 | -5.52 | -4.67 | 0.55 | -1.35 | 6.70 | -3.75 | -2.65 | -5.73 | -2.64 | 1.60 | -6.59 | -5.06 | -7.02 |
Endoplasmic Reticulum | 4.17 | 8.21 | 7.95 | 4.20 | -0.13 | 4.29 | 5.67 | 5.28 | 1.76 | -0.81 | 6.33 | 9.35 | 9.55 | 4.55 | -0.06 |
Endosome | -1.54 | -6.09 | -6.28 | -5.80 | -1.49 | -2.18 | 6.90 | 1.17 | 3.08 | -4.60 | -4.25 | 2.41 | -5.64 | -3.52 | -6.57 |
Golgi | -3.34 | -11.20 | 1.24 | 3.21 | 11.19 | 2.07 | 7.56 | -6.59 | -7.41 | -9.37 | -0.96 | -0.70 | -3.54 | -2.96 | -2.77 |
Lipid Particles | -4.37 | -9.23 | -8.36 | -7.68 | 4.84 | 1.79 | -9.80 | -7.77 | -7.87 | -6.52 | -2.36 | -13.14 | -11.32 | -11.07 | -6.33 |
Mitochondria | 0.08 | 2.73 | -0.72 | -0.84 | -2.77 | -4.28 | 4.73 | 4.94 | 5.31 | 0.80 | -2.20 | 3.50 | -0.44 | 1.33 | -2.93 |
Mitotic Spindle | -2.92 | -6.56 | -3.43 | -1.18 | 5.02 | -1.53 | -3.75 | -1.15 | 0.57 | 2.87 | -3.46 | -7.08 | -3.60 | -1.07 | 2.61 |
None | 2.13 | -2.74 | -0.89 | -1.16 | -0.09 | -1.97 | 1.64 | 1.58 | 2.21 | -0.04 | -1.70 | 2.06 | -0.78 | 0.48 | -1.04 |
Nuclear Periphery | 0.71 | -10.21 | -2.27 | -2.89 | 9.00 | 0.46 | 0.01 | 0.75 | 0.69 | 1.14 | 0.42 | -2.64 | -0.94 | -1.80 | 2.16 |
Nuclear Periphery Foci | -0.68 | -11.96 | -4.86 | -4.43 | 10.98 | 3.22 | -6.52 | -8.13 | -9.23 | -0.79 | 1.21 | -11.31 | -8.32 | -8.88 | 5.68 |
Nucleolus | -0.78 | -8.05 | -9.13 | -3.21 | -3.10 | -1.46 | -6.09 | -6.15 | 1.30 | 0.15 | -1.56 | -4.89 | -9.82 | 0.34 | -7.53 |
Nucleus | 0.45 | -6.71 | -7.39 | -7.45 | 3.20 | -2.19 | -3.37 | -1.82 | 0.92 | 1.91 | -2.70 | -10.04 | -7.23 | -3.93 | -0.43 |
Peroxisomes | -5.04 | 0.51 | -4.64 | -4.17 | -4.68 | -3.16 | 3.04 | -6.58 | -1.43 | -7.80 | -4.59 | 0.99 | -5.40 | -4.04 | -5.49 |
Vacuole | -4.93 | -12.37 | -25.84 | -24.54 | -1.27 | -6.59 | -12.63 | -9.10 | -5.71 | -1.24 | -9.80 | -19.26 | -26.13 | -23.01 | -13.24 |
Vacuole Periphery | 3.44 | -4.88 | 2.27 | -0.55 | 5.71 | 1.15 | 7.46 | 2.84 | 2.07 | -4.34 | 3.19 | 7.03 | 3.33 | 0.76 | -2.68 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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