Standard name
Human Ortholog
Description Delta(9) fatty acid desaturase; required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0.08 0.05 0.06 0 0.06 0.05 0.1 0.11 0.12 0 0 0 0.09 0.16 0.08 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0.08 0.12 0.13 0.1 0.09 0.09 0.06 0.1 0.11 0.16 0.18 0.12 0.12 0.23 0.26 0.3 0.22 0 0 0 0 0 0
Cytoplasm 0.12 0.15 0.07 0.13 0.14 0.23 0.26 0.22 0.26 0.19 0.25 0.23 0.07 0.07 0.12 0.59 0.51 0.56 0 0 0 0 0 0
Endoplasmic Reticulum 0.9 0.85 0.89 0.8 0.82 0.7 0.69 0.73 0.65 0.61 0.59 0.62 0.88 0.88 0.81 0 0 0 0.95 0.89 0.94 0.89 0.89 0.91
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 9 25 24 16 24 22 22 18 28 37 46 6 6 2 12 25 7 3 9 4 2 5 3
Bud 0 3 3 3 4 7 6 3 7 5 8 7 0 0 0 2 1 1 1 2 1 1 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 22 31 52 38 30 39 43 23 35 31 57 67 23 23 47 34 48 20 1 1 8 4 8 6
Cytoplasm 49 58 28 39 43 99 131 83 88 53 89 85 14 14 24 76 82 52 6 12 1 3 7 5
Endoplasmic Reticulum 370 326 370 236 255 307 346 277 219 171 208 232 169 167 168 4 5 4 398 353 409 214 379 367
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 2 0
Golgi 4 1 13 13 1 0 1 0 2 3 1 2 2 0 1 3 4 7 0 3 0 0 1 3
Mitochondria 0 2 0 0 0 1 0 1 1 2 4 6 0 0 0 8 5 3 0 0 2 3 5 2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0 0 0 1 0
Nuclear Periphery 2 1 7 2 0 3 10 9 8 3 9 9 0 2 1 1 0 0 0 1 1 0 1 0
Nucleolus 0 0 0 1 0 1 1 4 3 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 2 2 3 2 4 10 11 6 7 29 7 6 4 4 1 4 5 6 1 3 0 3 8 6
Unique Cell Count 412 382 418 296 312 436 504 377 339 282 351 376 191 190 208 129 160 93 418 396 434 240 429 404
Labelled Cell Count 458 433 501 358 353 491 571 428 388 326 421 462 218 216 244 146 179 101 418 396 434 240 429 404


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.8 23.1 21.0 25.2 24.7 24.4 25.1 26.0 27.0 26.3 26.7 26.6 20.3 20.3 20.2 12.6 15.5 17.7 19.9 19.4 20.1
Std Deviation (1e-4) 4.2 4.8 3.8 5.3 5.0 5.2 5.1 5.6 6.4 8.3 6.9 7.2 3.7 3.7 4.5 2.7 3.9 4.5 4.4 4.1 3.5
Intensity Change (Log2) – – – 0.26 0.23 0.21 0.26 0.3 0.36 0.32 0.34 0.34 -0.05 -0.05 -0.06 -0.74 -0.44 -0.25 -0.08 -0.11 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.1 -0.5 -0.3 -1.1 -0.1 -0.4 1.9 2.3 3.1 -1.5 -1.5 -2.9 1.3 3.7 0.6
Bud 0 0 0 0 0 0 0 1.8 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.2 -1.2 -1.7 -1.9 -3.1 -0.9 -0.6 1.5 2.1 -0.1 -0.1 3.3 3.8 5.0 2.3
Cytoplasm 2.9 3.2 6.6 7.7 6.2 7.3 4.9 7.2 6.4 0.3 0.3 2.1 13.2 12.2 11.8
Endoplasmic Reticulum -3.2 -2.6 -6.5 -7.2 -5.4 -7.9 -8.6 -9.4 -8.8 0 -0.2 -2.6 -18.2 -19.2 -16.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.9 -2.7 -3.7 -3.6 -3.5 -2.5 -1.8 -2.9 -2.7 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0.3 0.7 0.7 0 0.9 0.7 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 1.9 1.8 0 0 5.9 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 31.922 33.8933 34.0318 32.6969 30.1155 33.769 36.6535 40.6186 35.9571 34.7984 34.2505 38.2753 40.7437 40.9218 37.9013 36.5825 38.0598 40.5691
Actin 0.0048 0.0002 0.0041 0.0008 0.0014 0.0004 0.0056 0 0.0101 0 0.0011 0 0.0011 0.0001 0.0166 0 0.0307 0
Bud 0.0001 0 0.0001 0.0002 0.0003 0.0001 0.0001 0 0.0002 0 0 0 0.0001 0 0.0001 0 0.0002 0
Bud Neck 0.0004 0.0005 0.0012 0.0027 0.0143 0.0028 0.0002 0.0001 0.0003 0.0001 0.0001 0.0003 0.0002 0.0003 0.0007 0.0001 0.0022 0.0003
Bud Periphery 0.0002 0.0001 0.0005 0.002 0.0016 0.0002 0.0002 0 0.0003 0.0002 0.0001 0.0001 0.0001 0.0003 0.0002 0 0.0004 0
Bud Site 0.0007 0.0002 0.0002 0.0001 0.0002 0.0001 0.0008 0 0.0024 0 0.0001 0 0.0002 0 0.0012 0 0.0003 0
Cell Periphery 0.0064 0.0337 0.0223 0.0273 0.0181 0.0038 0.0058 0.0043 0.0075 0.0031 0.0064 0.0041 0.0042 0.0217 0.0086 0.0022 0.0239 0.0018
Cytoplasm 0.0566 0.0923 0.0374 0.034 0.1052 0.0395 0.0127 0.0065 0.0195 0.0028 0.0047 0.0024 0.0162 0.017 0.0148 0.001 0.0157 0.0024
Cytoplasmic Foci 0.009 0.0006 0.001 0.0003 0.0008 0.0039 0.0106 0.0001 0.0063 0 0.0087 0 0.003 0.0001 0.0042 0 0.004 0
Eisosomes 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0003 0 0.0003 0.0001 0.0001 0 0 0.0001 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.7843 0.7972 0.885 0.8844 0.7779 0.8886 0.8869 0.976 0.8928 0.9691 0.9218 0.9765 0.8862 0.9218 0.9017 0.9641 0.8638 0.9766
Endosome 0.0071 0.0007 0.0021 0.001 0.0028 0.002 0.0089 0.0002 0.006 0.0022 0.0048 0.0002 0.0141 0.0003 0.0042 0.0016 0.0018 0.0006
Golgi 0.0013 0.0003 0.0014 0.0003 0.0006 0.0005 0.0018 0.0002 0.0021 0.0013 0.0013 0 0.0008 0.0002 0.0034 0.0009 0.0022 0.0001
Lipid Particles 0.0112 0.0272 0.0112 0.0114 0.0094 0.0028 0.0081 0.0026 0.0069 0.0011 0.004 0.001 0.0049 0.0104 0.0079 0.005 0.0166 0.0009
Mitochondria 0.0028 0.0041 0.0016 0.0049 0.0022 0.0038 0.0032 0 0.011 0.0008 0.0034 0 0.0008 0 0.0025 0 0.0002 0
None 0.0046 0.0001 0.0002 0.0011 0.0137 0.0005 0.009 0 0.0071 0.0001 0.0125 0 0.0029 0.0001 0.0005 0 0.0003 0
Nuclear Periphery 0.0645 0.0214 0.0173 0.0148 0.0208 0.0337 0.0289 0.005 0.0141 0.0077 0.0157 0.0071 0.0353 0.0178 0.0179 0.0202 0.013 0.012
Nucleolus 0.0004 0.0001 0.0001 0.0001 0.0011 0.0001 0.0007 0 0.0004 0 0.0003 0 0.0002 0 0.0001 0 0.0001 0
Nucleus 0.0157 0.0021 0.0011 0.0013 0.0055 0.0068 0.0018 0.0001 0.001 0.0001 0.0007 0.0003 0.0042 0.0004 0.0005 0.0001 0.0004 0.0003
Peroxisomes 0.0017 0.0001 0.0007 0 0.0001 0.0004 0.0011 0 0.0013 0 0.0012 0 0.0004 0 0.0034 0 0.007 0
Punctate Nuclear 0.0082 0.0001 0.0003 0.0001 0.0005 0.0006 0.0056 0 0.0024 0 0.008 0 0.0071 0 0.0009 0 0.0058 0
Vacuole 0.0178 0.0188 0.0119 0.0121 0.0176 0.0088 0.0061 0.0046 0.0064 0.0072 0.0029 0.0075 0.0136 0.0089 0.0078 0.0035 0.0111 0.0044
Vacuole Periphery 0.0022 0.0002 0.0003 0.001 0.0059 0.0006 0.0011 0.0001 0.0016 0.004 0.0021 0.0004 0.0043 0.0003 0.0025 0.0012 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 365.6422 67.56 27.6922 36.8589 43.3943 405.0051 41.1486 29.4191 32.2029 43.088
Translational Efficiency 1.211 0.8593 0.7931 0.8837 1.3163 1.2583 0.865 0.6944 0.5981 0.8794

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
221 1642 266 1427 1970 1214 912 2046 2191 2856 1178 3473

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1795.92 2113.48 1812.24 2320.16 2449.84 1823.06 2033.63 2594.33 2383.88 1990.03 1983.64 2481.68
Standard Deviation 294.79 558.65 324.57 472.95 661.40 369.74 305.31 482.15 663.98 508.09 323.30 497.05
Intensity Change Log 2 — 0.234897 0.013051 0.369501 — -0.426325 -0.268630 0.082674 — -0.109095 -0.142713 0.211019

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002619 0.001635 0.000980 0.003102 0.000664 0.001263 0.001270 0.000829 0.000861 0.001477 0.001205 0.001763
Bud Neck 0.001934 0.000147 0.001966 0.006479 0.000154 0.000808 0.002120 0.001656 0.000334 0.000428 0.002085 0.003638
Bud Site 0.012120 0.001757 0.003043 0.030047 0.000602 0.007748 0.008870 0.006297 0.001764 0.004304 0.007554 0.016055
Cell Periphery 0.020805 0.007498 0.012927 0.036290 0.010847 0.006302 0.012209 0.031876 0.011851 0.006990 0.012371 0.033689
Cytoplasm 0.012369 0.001568 0.001975 0.024289 0.000786 0.007968 0.005156 0.007977 0.001954 0.004289 0.004438 0.014679
Cytoplasmic Foci 0.005127 0.000389 0.000267 0.004444 0.000217 0.003411 0.001304 0.001350 0.000712 0.001674 0.001069 0.002621
Eisosomes 0.000092 0.000096 0.000193 0.000234 0.000262 0.000145 0.000169 0.000202 0.000245 0.000117 0.000174 0.000215
Endoplasmic Reticulum 0.846134* 0.971152* 0.942673* 0.684605* 0.970119* 0.939296* 0.921604* 0.876374* 0.957613* 0.957611* 0.926361* 0.797579*
Endosome 0.014532 0.003341 0.006928 0.017438 0.002572 0.006857 0.008261 0.006425 0.003778 0.004835 0.007960 0.010950
Golgi 0.010006 0.001957 0.001697 0.013302 0.002808 0.004466 0.003084 0.005173 0.003534 0.003023 0.002770 0.008513
Lipid Particles 0.020500 0.003147 0.010507 0.105948* 0.003309 0.005532 0.017735 0.033216 0.005043 0.004160 0.016103 0.063100
Mitochondria 0.005209 0.000262 0.000020 0.001169 0.000353 0.001246 0.000130 0.000858 0.000843 0.000680 0.000105 0.000986
Mitotic Spindle 0.001178 0.000073 0.000092 0.003839 0.000056 0.001072 0.000602 0.000218 0.000169 0.000498 0.000487 0.001705
None 0.000041 0.000986 0.000006 0.000925 0.000306 0.000403 0.000086 0.001080 0.000279 0.000738 0.000068 0.001016
Nuclear Periphery 0.000864 0.000549 0.001113 0.001969 0.000942 0.000275 0.001067 0.002022 0.000934 0.000433 0.001078 0.002000
Nuclear Periphery Foci 0.006620 0.001440 0.007755 0.023749 0.000845 0.001840 0.006624 0.003877 0.001427 0.001610 0.006879 0.012042
Nucleolus 0.000013 0.000009 0.000001 0.000030 0.000006 0.000335 0.000004 0.000019 0.000007 0.000148 0.000003 0.000023
Nucleus 0.000593 0.000125 0.000105 0.001433 0.000062 0.001379 0.000300 0.000335 0.000116 0.000658 0.000256 0.000786
Peroxisomes 0.000297 0.000132 0.000017 0.000561 0.000239 0.000577 0.000077 0.000507 0.000244 0.000321 0.000064 0.000529
Vacuole 0.032701 0.003188 0.007584 0.036377 0.003208 0.007171 0.008918 0.017963 0.006183 0.004881 0.008617 0.025529
Vacuole Periphery 0.006247 0.000548 0.000152 0.003770 0.001643 0.001906 0.000411 0.001749 0.002107 0.001125 0.000353 0.002580

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.59 1.01 -0.36 -1.94 -3.13 -1.21 -1.50 -0.59 0.89 1.26 -1.08 -0.88 -2.46 -0.80 -1.90
Bud Neck 3.78 -0.05 -6.47 -10.50 -5.63 -2.05 -5.13 -4.51 -1.98 1.01 -0.57 -5.25 -10.43 -9.67 -3.77
Bud Site 2.44 2.10 -3.95 -10.53 -9.59 -4.71 -4.50 -5.75 0.58 1.27 -2.97 -3.88 -10.91 -8.51 -4.48
Cell Periphery 2.75 1.48 -2.85 -11.31 -6.90 3.54 -0.88 -10.60 -13.02 -8.85 4.34 -0.39 -12.57 -17.29 -11.43
Cytoplasm 2.03 1.95 -2.35 -10.77 -10.39 -5.99 -3.72 -6.96 -0.21 -1.56 -2.91 -2.48 -10.75 -9.10 -7.10
Cytoplasmic Foci 2.39 2.46 0.34 -6.94 -7.38 -4.43 -2.36 -4.57 2.74 -0.03 -2.55 -0.91 -5.57 -2.19 -3.36
Eisosomes -0.04 -1.18 -3.52 -4.68 -0.51 2.03 1.85 1.32 -1.19 -0.83 3.19 1.51 0.75 -3.78 -1.17
Endoplasmic Reticulum -6.44 -4.67 8.04 30.76* 22.16 5.55 7.64 17.43 8.89 5.38 0.06 5.60 28.12 28.66 17.99
Endosome 4.45 2.82 -1.18 -16.49 -7.99 -5.81 -6.88 -9.87 0.49 2.12 -2.26 -5.67 -14.25 -11.70 -3.85
Golgi 3.41 3.54 -1.15 -8.20 -8.49 -1.84 -0.41 -4.53 -0.74 -3.07 0.94 1.40 -6.96 -7.46 -7.72
Lipid Particles 3.47 1.64 -11.91 -19.78 -15.20 -1.85 -5.82 -12.42 -11.02 -4.50 0.97 -5.13 -21.43 -22.26 -14.10
Mitochondria 1.34 1.40 1.08 -4.59 -5.97 -2.15 2.52 -3.09 0.83 -4.63 0.34 1.91 -0.48 -1.50 -7.16
Mitotic Spindle 1.67 1.66 -2.63 -4.27 -4.26 -1.58 -1.74 -4.04 1.28 1.25 -1.19 -1.36 -4.22 -2.84 -2.55
None -2.26 2.87 -2.99 0.11 -3.12 -1.00 3.19 -2.89 -2.42 -3.76 -1.86 3.55 -3.64 -0.88 -4.78
Nuclear Periphery 1.94 -1.15 -6.65 -9.87 -4.41 8.45 -1.08 -7.62 -12.84 -5.12 6.19 -1.37 -10.27 -15.11 -6.69
Nuclear Periphery Foci 3.56 -0.45 -7.85 -11.81 -6.42 -2.62 -6.06 -5.72 -3.68 2.60 -0.41 -6.22 -12.43 -12.00 -4.74
Nucleolus 0.68 2.87 -3.72 -4.66 -8.95 -1.30 1.45 -5.74 1.26 -6.63 -1.31 2.77 -8.58 1.18 -10.60
Nucleus 2.01 2.11 -3.74 -6.19 -6.28 -1.69 -3.44 -5.20 1.32 0.01 -1.63 -2.61 -6.99 -1.03 -5.07
Peroxisomes 0.80 1.56 -1.00 -2.23 -3.14 -2.03 2.56 -2.24 0.50 -4.13 -0.98 3.07 -2.56 -1.48 -4.95
Vacuole 4.57 3.85 -0.98 -15.06 -12.22 -3.11 -4.43 -11.14 -6.14 -4.71 1.28 -2.16 -14.21 -15.98 -10.90
Vacuole Periphery 3.34 3.60 1.36 -6.66 -7.98 -0.42 2.15 -0.23 0.32 -5.57 1.71 3.23 -0.89 -5.00 -9.39
Standard name
Human Ortholog
Description Delta(9) fatty acid desaturase; required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Ole1

Ole1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ole1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available