Standard name
Human Ortholog
Description Subunit of DUBm module of SAGA and SLIK; has roles in anchoring deubiquitination module (DUBm) into SAGA and SLIK complexes, maintaining organization and ubiquitin-binding conformation of Ubp8p, thereby contributing to overall DUBm activity; involved in preinitiation complex assembly at promoters; relocalizes to cytosol under hypoxia; human homolog ATXN7 implicated in spinocerebellar ataxia, and can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.06 0 0.05 – 0 0 0.08 0 0 0.16 0.17 0.08 0.07 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0 0 – 0.1 0.09 0.16 0.07 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.92 0.92 0.88 0.91 0.92 0.88 0.87 – 0.83 0.78 0.75 0.79 0.9 0.82 0.78 0.86 0.86 0.78 0.88 0.87 0.85 0.83 0.83 0.79
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.29 0 0.21 0 0 0.14 0.13 – 0.23 0.21 0.33 0.26 0.14 0 0.05 0.09 0.08 0.1 0.1 0.05 0.08 0.09 0.07 0.11
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Bud 1 0 0 0 0 0 0 – 0 2 0 3 0 0 0 1 2 0 0 0 0 0 2 1
Bud Neck 0 0 0 0 0 0 1 – 1 4 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 6 – 3 3 5 6 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1 1 12 25 13 24 – 8 18 16 9 1 46 55 23 21 15 0 3 0 0 1 1
Endoplasmic Reticulum 0 1 0 0 0 0 0 – 0 0 0 0 0 1 1 1 4 4 0 0 0 0 0 0
Endosome 0 0 0 0 1 0 1 – 0 1 0 0 0 0 0 1 4 0 0 0 0 0 6 1
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 0 1 1 1 1 0 0 1 0 0 0 2
Mitochondria 7 1 1 2 0 15 15 – 35 36 33 23 0 1 0 2 8 7 0 1 0 1 0 1
Nucleus 122 83 38 191 389 303 427 – 292 328 151 248 26 238 259 247 271 121 117 107 73 70 219 206
Nuclear Periphery 0 0 0 0 0 0 1 – 2 4 0 1 0 4 1 0 1 0 0 0 0 0 0 0
Nucleolus 38 4 9 6 15 47 63 – 79 87 67 82 4 11 18 26 26 15 13 5 6 7 17 29
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 3 2 1 5 – 2 5 1 2 2 3 4 1 0 1 0 0 0 0 2 4
Vac/Vac Membrane 0 3 0 2 9 5 5 – 8 7 3 6 0 3 8 3 7 10 2 2 1 1 14 7
Unique Cell Count 133 90 43 210 425 343 491 350 422 201 312 29 292 331 288 316 155 134 124 86 85 265 261
Labelled Cell Count 168 94 49 216 441 385 548 430 495 278 381 33 308 349 306 346 173 134 124 86 85 265 261


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 8.8 7.3 8.5 9.0 7.3 7.3 – 6.2 6.9 6.2 6.5 9.1 8.9 8.5 10.8 10.8 10.3 6.5 7.8 8.2
Std Deviation (1e-4) 1.2 1.6 1.4 2.3 2.4 2.1 2.2 – 1.9 2.0 2.1 1.8 2.0 2.4 2.5 2.6 2.8 2.8 0.9 1.5 1.5
Intensity Change (Log2) – – – 0.22 0.3 -0.01 -0.0 – -0.23 -0.08 -0.25 -0.17 0.32 0.29 0.21 0.56 0.57 0.5 -0.18 0.1 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 2.4 2.5 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 2.4 0 0 0 0 0 0 0
Nucleus 0.5 0.7 0 -0.3 – -0.8 -1.6 -1.9 -1.4 0.2 -1.1 -1.5 -0.5 -0.5 -1.5
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 -1.3 -1.5 – 0.2 0 1.6 0.8 -0.8 0 0 0 0 -2.0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8215 2.7062 2.2936 1.9985 1.9526 2.1698 4.9829 4.6095 4.0466 3.7567 4.5779 3.9162 3.5138 3.0955 2.8652 3.0185 2.632 3.0035
Actin 0.0014 0.0001 0.0113 0.0244 0.0001 0.003 0.0085 0 0.0001 0.0001 0.0179 0.0008 0.0232 0 0.0002 0 0.0093 0.0009
Bud 0.0001 0 0.0005 0.0003 0.0001 0.0001 0.0003 0 0.0001 0 0.0009 0.0017 0.0002 0 0 0 0.0002 0
Bud Neck 0.0007 0.0001 0.0036 0.0003 0.0005 0.0007 0.0004 0 0 0 0.0019 0.0005 0.0007 0 0 0 0.0024 0.0005
Bud Periphery 0.0001 0 0.0008 0.0005 0.0001 0.0002 0.0004 0 0.0001 0 0.002 0.0019 0.0004 0 0 0 0.0005 0
Bud Site 0.004 0.0001 0.0056 0.0004 0.0001 0.0002 0.001 0 0.0002 0 0.0042 0.002 0.0004 0 0.0001 0 0.0003 0.0001
Cell Periphery 0.0001 0 0.0005 0.0001 0 0 0.0002 0 0 0 0.0006 0.0001 0.0001 0 0 0 0.0003 0
Cytoplasm 0.012 0 0.0024 0.0009 0.0001 0.0002 0.0147 0 0.0005 0.0018 0.0067 0.0016 0.0014 0 0.0006 0 0.0009 0.0002
Cytoplasmic Foci 0.0091 0 0.0049 0.0162 0 0.0032 0.0043 0 0.0001 0.0041 0.02 0.0003 0.0041 0 0.001 0 0.0003 0.0004
Eisosomes 0 0 0.0001 0.0001 0 0 0 0 0 0 0.0002 0 0.0004 0 0 0 0.0003 0.0001
Endoplasmic Reticulum 0.0039 0 0.0006 0.0002 0 0.0003 0.0099 0 0.0002 0.0009 0.0023 0.0003 0.001 0 0.0001 0 0.0048 0.0001
Endosome 0.0037 0.0001 0.0102 0.0599 0.0001 0.0028 0.0074 0 0.0003 0.0018 0.0231 0.0009 0.007 0 0.001 0 0.0096 0.0005
Golgi 0.003 0.0002 0.0032 0.0142 0 0.0022 0.0007 0 0 0.0002 0.0082 0.0002 0.0019 0 0.0001 0 0.002 0.0002
Lipid Particles 0.004 0.0001 0.007 0.0022 0 0.0125 0.0019 0 0 0.0023 0.0112 0 0.0032 0 0.0002 0 0.0011 0.0008
Mitochondria 0.0006 0.0118 0.0111 0.0562 0.0008 0.0011 0.0015 0.0001 0.0009 0.0001 0.0103 0.0007 0.0042 0 0.0001 0.0001 0.003 0.0001
None 0.001 0 0.0118 0.0001 0.0001 0.0002 0.002 0 0.0001 0.0008 0.0048 0.0001 0.0015 0 0.0013 0 0.0023 0.0017
Nuclear Periphery 0.0029 0.0005 0.0016 0.0002 0.0028 0.0045 0.0269 0.0001 0.0024 0.0072 0.0153 0.0004 0.0049 0.0001 0.0005 0.0001 0.0082 0.0006
Nucleolus 0.0277 0.0243 0.0175 0.0154 0.1215 0.1449 0.0155 0.0141 0.0215 0.0083 0.1049 0.0457 0.0202 0.0198 0.0133 0.0129 0.1559 0.0524
Nucleus 0.9178 0.9615 0.9014 0.7703 0.8668 0.8146 0.8961 0.9787 0.9724 0.953 0.7016 0.9411 0.9102 0.9798 0.9799 0.9867 0.7934 0.9376
Peroxisomes 0.0021 0.0002 0.0033 0.0356 0 0.0016 0.0002 0 0 0 0.0018 0.0001 0.0009 0 0.0002 0 0.0001 0.0002
Punctate Nuclear 0.0048 0.0009 0.0012 0.0002 0.0067 0.0072 0.005 0.0068 0.0003 0.019 0.0307 0.0002 0.0126 0.0001 0.0011 0.0001 0.0028 0.0036
Vacuole 0.0008 0.0001 0.0011 0.0013 0.0002 0.0002 0.0015 0 0.0004 0.0002 0.0207 0.0011 0.0009 0 0.0001 0 0.0012 0.0001
Vacuole Periphery 0.0003 0.0001 0.0004 0.0012 0.0002 0.0003 0.0016 0 0.0005 0.0002 0.0107 0.0002 0.0007 0 0 0 0.0012 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.2666 6.9956 9.6405 14.1365 11.958 13.9665 14.1464 13.8103 13.3498 14.9182
Translational Efficiency 2.2665 2.4829 1.719 1.1753 1.2854 1.2609 1.2459 1.4951 1.632 1.4481

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1779 1181 2300 1071 2210 1384 161 2259 3989 2565 2461 3330

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 687.96 893.07 1019.83 1070.41 874.54 843.27 1144.16 1028.63 791.33 866.20 1027.96 1042.07
Standard Deviation 98.75 165.50 157.10 167.73 127.47 145.74 151.18 167.94 148.16 157.12 159.70 169.00
Intensity Change Log 2 — 0.376449 0.567932 0.637767 — -0.052530 0.387693 0.234128 — 0.152193 0.469838 0.425873

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000185 0.000561 0.000623 0.000955 0.000321 0.000460 0.001460 0.000831 0.000260 0.000507 0.000678 0.000871
Bud Neck 0.003477 0.007528 0.025296 0.024080 0.006685 0.007802 0.012328 0.035173 0.005254 0.007676 0.024448 0.031605
Bud Site 0.000920 0.000709 0.001714 0.002466 0.000544 0.000685 0.001401 0.003935 0.000712 0.000696 0.001693 0.003462
Cell Periphery 0.000286 0.000101 0.000173 0.000105 0.000042 0.000060 0.000039 0.000152 0.000151 0.000079 0.000164 0.000137
Cytoplasm 0.006335 0.001733 0.003251 0.004025 0.000517 0.001767 0.000267 0.003879 0.003112 0.001751 0.003056 0.003926
Cytoplasmic Foci 0.001040 0.000400 0.000534 0.001252 0.000124 0.000179 0.000045 0.000052 0.000532 0.000281 0.000502 0.000438
Eisosomes 0.000024 0.000038 0.000059 0.000044 0.000037 0.000034 0.000063 0.000043 0.000031 0.000035 0.000059 0.000043
Endoplasmic Reticulum 0.002102 0.002625 0.001920 0.003462 0.002794 0.002139 0.003730 0.003540 0.002485 0.002362 0.002039 0.003515
Endosome 0.000299 0.000426 0.000323 0.001730 0.000190 0.000260 0.000099 0.000298 0.000238 0.000336 0.000308 0.000759
Golgi 0.000168 0.000217 0.000362 0.001529 0.000128 0.000103 0.000125 0.000091 0.000146 0.000155 0.000346 0.000553
Lipid Particles 0.000179 0.000173 0.000045 0.000236 0.000088 0.000092 0.000113 0.000057 0.000129 0.000129 0.000049 0.000115
Mitochondria 0.001332 0.001720 0.001268 0.002052 0.001105 0.000585 0.001292 0.002859 0.001206 0.001108 0.001269 0.002600
Mitotic Spindle 0.000223 0.001131 0.002390 0.005496 0.001090 0.000348 0.000482 0.004267 0.000703 0.000709 0.002266 0.004662
None 0.027268 0.007651 0.003475 0.005205 0.001121 0.003498 0.001943 0.005000 0.012782 0.005410 0.003375 0.005066
Nuclear Periphery 0.000382 0.000124 0.000531 0.000857 0.000180 0.000107 0.000145 0.000603 0.000270 0.000115 0.000506 0.000685
Nuclear Periphery Foci 0.000383 0.000563 0.000184 0.000810 0.000296 0.000118 0.000070 0.000144 0.000335 0.000323 0.000177 0.000358
Nucleolus 0.051277 0.068026 0.038704 0.009803 0.056057 0.058884 0.030542 0.012982 0.053925 0.063093 0.038170 0.011959
Nucleus 0.903097* 0.904663* 0.918190* 0.932661* 0.927578* 0.921305* 0.943306* 0.922725* 0.916660* 0.913642* 0.919833* 0.925920*
Peroxisomes 0.000314 0.000647 0.000364 0.000314 0.000360 0.000530 0.001250 0.000176 0.000340 0.000584 0.000422 0.000221
Vacuole 0.000447 0.000497 0.000415 0.002563 0.000320 0.000733 0.001163 0.002237 0.000376 0.000624 0.000464 0.002342
Vacuole Periphery 0.000263 0.000468 0.000178 0.000356 0.000424 0.000313 0.000136 0.000956 0.000352 0.000385 0.000176 0.000763

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -12.15 -21.83 -20.74 -12.31 -10.09 -5.84 -6.60 -17.03 -13.17 3.55 -12.73 -19.05 -26.71 -17.10 -7.32
Bud Neck -5.47 -24.28 -17.62 -13.85 -1.92 -1.63 -4.38 -21.45 -19.45 -7.27 -4.82 -23.35 -27.51 -23.72 -6.29
Bud Site 0.92 -2.83 -5.60 -6.92 -3.00 -1.05 -5.29 -7.35 -6.98 -4.09 0.09 -4.34 -8.64 -8.86 -4.63
Cell Periphery 3.73 2.19 4.00 0.05 1.30 -1.48 -0.48 -7.05 -4.34 -6.99 3.11 0.10 1.46 -3.01 0.74
Cytoplasm 4.35 3.46 2.63 -1.95 -0.85 -2.31 0.98 -5.54 -2.34 -6.15 2.33 0.90 -0.68 -3.09 -1.54
Cytoplasmic Foci 1.67 1.99 1.19 -0.45 -0.68 -0.97 1.34 1.09 1.31 -0.76 0.90 1.06 1.84 0.79 0.84
Eisosomes -7.23 -17.79 -13.32 -5.22 4.12 1.68 -6.83 -3.12 -5.11 5.56 -3.39 -15.55 -10.21 -6.62 7.84
Endoplasmic Reticulum -2.71 -0.04 -9.64 -7.58 -11.77 4.17 -3.11 -5.02 -9.86 0.56 0.93 2.18 -9.76 -11.62 -13.13
Endosome -0.84 -0.07 -1.60 -1.20 -1.56 -0.65 1.69 -2.43 -0.40 -4.42 -1.08 -0.63 -2.00 -1.09 -1.50
Golgi -0.74 -0.71 -1.15 -1.05 -0.86 0.57 -0.85 0.34 -0.48 2.16 -0.33 -0.84 -1.08 -1.00 -0.19
Lipid Particles -0.12 4.16 -0.27 -0.00 -3.66 -0.66 -0.86 0.40 0.81 1.28 -0.54 4.09 0.57 0.75 -4.29
Mitochondria -0.46 -0.20 -2.66 -1.37 -6.59 2.07 -1.73 -5.51 -10.95 -4.28 0.23 -0.86 -5.45 -4.87 -8.13
Mitotic Spindle -1.80 -3.95 -4.22 -3.60 -2.95 1.48 0.50 -3.94 -4.87 -4.33 -0.19 -2.94 -5.92 -5.81 -3.73
None 7.05 9.88 8.98 2.05 -0.86 -4.39 -1.59 -6.43 -1.17 -3.38 5.62 8.27 7.13 1.28 -1.99
Nuclear Periphery 1.46 -0.69 -2.36 -4.16 -2.05 2.04 -0.64 -9.36 -11.62 -8.58 1.90 -1.72 -5.17 -9.12 -2.48
Nuclear Periphery Foci -0.77 1.76 -0.43 0.13 -1.23 1.91 2.23 0.94 -1.38 -1.84 0.03 2.06 0.23 0.17 -1.31
Nucleolus -4.77 2.70 17.22 18.83 14.93 -0.99 4.38 19.63 17.02 4.15 -4.05 4.74 25.45 24.83 14.93
Nucleus -0.40 -1.74 -2.99 -2.61 -1.80 1.90 -0.52 3.67 1.61 2.44 0.96 1.46 0.14 -0.78 -1.26
Peroxisomes -2.47 -0.82 -0.29 3.43 1.76 -3.04 -3.97 3.29 6.37 4.61 -3.66 -2.25 1.52 6.88 7.41
Vacuole -0.37 -0.02 -5.11 -4.94 -5.21 -0.75 -4.36 -5.68 -2.88 -2.76 -0.84 -1.21 -7.46 -5.07 -7.05
Vacuole Periphery -1.19 1.27 -0.32 1.05 -1.75 0.49 1.70 -0.71 -0.98 -1.50 -0.24 1.94 -0.84 -0.66 -1.60
Standard name
Human Ortholog
Description Subunit of DUBm module of SAGA and SLIK; has roles in anchoring deubiquitination module (DUBm) into SAGA and SLIK complexes, maintaining organization and ubiquitin-binding conformation of Ubp8p, thereby contributing to overall DUBm activity; involved in preinitiation complex assembly at promoters; relocalizes to cytosol under hypoxia; human homolog ATXN7 implicated in spinocerebellar ataxia, and can complement yeast null mutant
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Sgf73

Sgf73


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sgf73-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available