Standard name
Human Ortholog
Description NADH diphosphatase (pyrophosphatase); hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.96 0.94 0.82 0.75 0.64 0.65 0.61 0.64 0.56 0.98 0.99 0.99 0.95 0.97 0.9 0.96 0.94 0.96 0.95 0.95 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.06 0.19 0.25 0.27 0.31 0.36 0.41 0.41 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.1 0.09 0.17 0.15 0.18 0.1 0.15 0 0 0 0.08 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 1 0 3 4 2 7 6 4 0 0 0 0 0 0 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 3 2 2 5 7 11 4 9 9 19 2 2 0 4 3 1 0 0 0 0 0 0
Cytoplasm 171 122 167 214 238 268 305 334 230 273 227 244 328 650 687 102 88 46 163 115 160 200 469 497
Endoplasmic Reticulum 0 1 2 1 2 0 2 3 6 6 0 3 4 2 1 3 1 2 0 0 1 0 3 3
Endosome 0 0 0 1 0 0 1 5 2 0 0 0 0 1 1 0 0 0 0 1 0 1 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 2
Mitochondria 3 0 1 13 14 62 101 140 108 163 147 180 0 0 0 1 0 0 0 1 1 2 1 1
Nucleus 0 0 0 0 0 3 8 11 7 8 5 11 4 4 4 0 3 2 0 2 0 2 5 3
Nuclear Periphery 1 1 1 0 0 2 2 1 0 3 1 7 0 0 0 0 0 0 1 0 0 1 0 1
Nucleolus 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Vac/Vac Membrane 0 1 5 6 8 31 37 87 53 79 34 66 0 4 16 9 2 3 0 1 1 0 2 3
Unique Cell Count 175 124 171 223 252 325 409 519 352 448 355 435 333 657 696 107 91 51 170 123 168 212 493 528
Labelled Cell Count 176 127 179 237 265 371 467 596 413 550 429 536 338 663 710 119 97 55 170 123 168 212 493 528


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 8.2 7.4 7.2 7.2 6.6 6.4 6.5 6.3 6.0 6.0 5.9 9.3 9.1 9.0 9.0 9.0 8.8 8.9 9.0 9.3
Std Deviation (1e-4) 0.9 1.1 1.6 1.2 1.6 1.4 1.4 1.4 1.4 1.2 1.4 1.4 1.2 1.2 1.2 1.8 1.7 1.2 1.2 1.5 1.4
Intensity Change (Log2) – – – -0.04 -0.03 -0.17 -0.21 -0.18 -0.22 -0.3 -0.31 -0.33 0.34 0.31 0.29 0.29 0.28 0.25 0.27 0.29 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1.5 0 0 0 0 0 0
Cytoplasm -0.9 -1.6 -4.9 -6.5 -8.5 -8.1 -9.0 -8.4 -9.9 0.7 1.3 1.0 -1.1 -0.5 -2.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.8 2.7 5.9 7.0 7.4 7.9 9.0 9.8 9.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.1 2.7 2.6 4.6 4.1 4.8 2.7 4.2 0 0 0 2.0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6994 3.7721 3.128 2.5738 2.5348 3.1442 3.5694 5.2802 4.6099 4.12 4.1218 4.6223 2.8786 4.1405 3.5576 3.7297 3.0614 3.2188
Actin 0.0019 0.0006 0.0014 0.0003 0.0003 0.0005 0.0268 0.0121 0.0194 0.023 0.0048 0.0029 0.0186 0.0001 0.0065 0.0549 0 0.0011
Bud 0.0042 0.0006 0.0055 0.0002 0.0001 0.0001 0.0012 0.0004 0.0021 0.0014 0.0004 0.0002 0.0003 0.0001 0.0001 0.0011 0.0001 0.0002
Bud Neck 0.0006 0.0002 0.0006 0.0009 0.0005 0.0007 0.0021 0.0002 0.0025 0.0018 0.0016 0.0015 0.0002 0.0001 0.0002 0.0014 0.0002 0.0017
Bud Periphery 0.0009 0.0002 0.0011 0 0 0 0.0012 0.0001 0.0033 0.001 0.0003 0.0002 0.0003 0 0.0001 0.0012 0 0
Bud Site 0.0102 0.0012 0.0044 0.0003 0.0001 0 0.0044 0.0008 0.0152 0.0085 0.0073 0.0003 0.0008 0.0056 0.0003 0.002 0 0.0001
Cell Periphery 0.0001 0 0.0001 0 0 0 0.0002 0 0.0003 0.0001 0.0001 0 0.0001 0 0 0.0002 0 0
Cytoplasm 0.8765 0.9047 0.8863 0.9001 0.9315 0.9122 0.7906 0.9584 0.8615 0.8654 0.8265 0.9212 0.8668 0.9666 0.9558 0.7093 0.9242 0.9576
Cytoplasmic Foci 0.008 0.0109 0.0101 0.0114 0.0057 0.012 0.0515 0.0047 0.0189 0.0164 0.0349 0.0099 0.0179 0.0039 0.0039 0.026 0.0048 0.012
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0.0001 0.0001 0 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0035 0.0033 0.0032 0.0035 0.0034 0.0016 0.0054 0.0012 0.002 0.0024 0.0022 0.0024 0.0033 0.0007 0.0009 0.0009 0.0023 0.0014
Endosome 0.008 0.0041 0.0066 0.0093 0.0038 0.0053 0.0281 0.0011 0.0143 0.0264 0.0332 0.0159 0.0128 0.0016 0.0008 0.0387 0.0071 0.0025
Golgi 0.0005 0.0002 0.0005 0.0002 0.0002 0.0002 0.006 0.0005 0.0049 0.0137 0.0156 0.0051 0.0027 0.0003 0.0011 0.0228 0.0001 0.0002
Lipid Particles 0.0004 0.0001 0.0001 0 0 0.0001 0.0047 0 0.0017 0.0035 0.0373 0.004 0.0059 0 0.0005 0.0235 0.0001 0
Mitochondria 0.0006 0.0002 0.0004 0.0005 0.0002 0.0003 0.0027 0.0003 0.0079 0.0051 0.0029 0.0032 0.0017 0.0001 0.0003 0.0082 0.0001 0.0003
None 0.062 0.0426 0.0595 0.0405 0.0174 0.05 0.0241 0.0135 0.0193 0.0044 0.0138 0.0098 0.02 0.0176 0.0169 0.0021 0.0513 0.0079
Nuclear Periphery 0.0036 0.0025 0.0021 0.0042 0.0021 0.0014 0.0077 0.0008 0.0016 0.0047 0.0031 0.0023 0.0043 0.0005 0.0005 0.0093 0.0015 0.0013
Nucleolus 0.0001 0.0001 0.0001 0.0001 0 0 0.0003 0 0.0009 0.0002 0.0004 0.0002 0.0003 0 0 0.0026 0 0
Nucleus 0.0141 0.023 0.0141 0.0226 0.0315 0.0116 0.0123 0.0044 0.0096 0.0118 0.0043 0.011 0.0377 0.0021 0.0034 0.0478 0.0027 0.0099
Peroxisomes 0.001 0.0004 0.0003 0.0002 0.0001 0.0005 0.0206 0.0002 0.0091 0.004 0.0062 0.0049 0.0015 0 0.008 0.0354 0 0.0017
Punctate Nuclear 0.002 0.0039 0.0025 0.0027 0.0019 0.0025 0.0069 0.0008 0.0015 0.0038 0.0033 0.0025 0.0032 0.0002 0.0002 0.0013 0.0003 0.0016
Vacuole 0.0014 0.0009 0.001 0.0024 0.001 0.0008 0.0024 0.0004 0.003 0.0016 0.0011 0.0018 0.0012 0.0004 0.0003 0.0077 0.0046 0.0004
Vacuole Periphery 0.0003 0.0002 0.0002 0.0007 0.0002 0.0002 0.0008 0.0001 0.0009 0.0006 0.0007 0.0007 0.0005 0 0.0001 0.0035 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.8558 27.3282 22.8135 37.1635 32.2043 26.7344 35.6187 37.2988 29.965 42.9684
Translational Efficiency 0.8166 0.9078 1.1089 0.6292 0.8129 1.4917 0.8851 0.7159 1.0276 0.745

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2264 376 2553 678 2149 1089 355 1011 4413 1465 2908 1689

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 899.46 968.33 1132.61 1084.42 851.54 916.63 1188.12 1098.02 876.12 929.90 1139.39 1092.56
Standard Deviation 111.69 148.78 137.98 153.94 108.73 121.65 153.07 163.49 112.83 131.11 141.08 159.86
Intensity Change Log 2 — 0.106440 0.332520 0.269793 — 0.106265 0.480534 0.366758 — 0.106355 0.406400 0.317763

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000178 0.000285 0.000235 0.001174 0.000083 0.000108 0.000446 0.005443 0.000132 0.000154 0.000261 0.003729
Bud Neck 0.002750 0.005500 0.001970 0.006062 0.001910 0.002586 0.002045 0.003666 0.002341 0.003334 0.001979 0.004628
Bud Site 0.005165 0.013632 0.007619 0.026600 0.002355 0.004931 0.007716 0.028663 0.003796 0.007164 0.007631 0.027835
Cell Periphery 0.000057 0.000103 0.000046 0.000110 0.000061 0.000050 0.000169 0.000179 0.000059 0.000064 0.000061 0.000151
Cytoplasm 0.721716* 0.670571* 0.707355* 0.540497* 0.730672* 0.733031* 0.562925* 0.588235* 0.726077* 0.717000* 0.689724* 0.569072*
Cytoplasmic Foci 0.094869 0.134008 0.005585 0.029717 0.077539 0.059790 0.013892 0.021702 0.086430 0.078838 0.006599 0.024920
Eisosomes 0.000025 0.000034 0.000007 0.000029 0.000022 0.000019 0.000043 0.000032 0.000024 0.000023 0.000011 0.000031
Endoplasmic Reticulum 0.000900 0.001034 0.000669 0.002210 0.001435 0.000585 0.002934 0.002322 0.001161 0.000700 0.000945 0.002277
Endosome 0.003625 0.011912 0.000737 0.007499 0.003033 0.004480 0.003624 0.006794 0.003337 0.006387 0.001090 0.007077
Golgi 0.001513 0.001857 0.000105 0.004352 0.000897 0.000969 0.000225 0.002197 0.001213 0.001197 0.000120 0.003062
Lipid Particles 0.001395 0.001424 0.000351 0.002959 0.001446 0.000879 0.001404 0.002271 0.001419 0.001019 0.000479 0.002547
Mitochondria 0.002129 0.001392 0.000494 0.000952 0.001767 0.001568 0.003007 0.001867 0.001953 0.001523 0.000800 0.001500
Mitotic Spindle 0.003177 0.003624 0.005679 0.072122 0.001069 0.009900 0.001580 0.043899 0.002151 0.008289 0.005179 0.055228
None 0.002939 0.002662 0.006040 0.003664 0.003556 0.002813 0.011275 0.007966 0.003240 0.002774 0.006679 0.006239
Nuclear Periphery 0.000190 0.000192 0.000703 0.001109 0.000247 0.000279 0.001052 0.000758 0.000218 0.000257 0.000746 0.000899
Nuclear Periphery Foci 0.000988 0.003688 0.000843 0.007272 0.001170 0.001028 0.007266 0.004024 0.001077 0.001711 0.001627 0.005328
Nucleolus 0.000279 0.000431 0.000138 0.000311 0.000474 0.000908 0.000168 0.000151 0.000374 0.000785 0.000141 0.000215
Nucleus 0.111444 0.057344 0.220743* 0.103703 0.133487* 0.116201 0.195105* 0.134796* 0.122178 0.101095 0.217613* 0.122315
Peroxisomes 0.000714 0.003890 0.000320 0.000520 0.000539 0.000748 0.000155 0.001912 0.000628 0.001554 0.000300 0.001353
Vacuole 0.045451 0.085686 0.040033 0.187313 0.037562 0.058293 0.184414 0.138665 0.041609 0.065323 0.057659 0.158193
Vacuole Periphery 0.000497 0.000732 0.000329 0.001823 0.000675 0.000833 0.000556 0.004456 0.000584 0.000807 0.000357 0.003399

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.68 -1.00 -4.34 -3.23 -3.14 -1.31 -11.37 -1.94 -1.91 -1.28 -0.71 -2.45 -2.38 -2.33 -1.94
Bud Neck -1.45 0.69 -1.86 0.82 -2.38 -1.36 -3.25 -2.53 -0.41 0.64 -1.60 -0.88 -2.74 0.14 -1.96
Bud Site -3.66 -0.38 -3.40 0.38 -3.17 -2.32 -6.44 -5.57 -3.55 -2.08 -3.19 -2.55 -6.14 -3.10 -4.21
Cell Periphery -4.42 1.67 -8.12 0.19 -9.78 2.70 -10.60 -7.66 -8.92 3.85 -1.08 -2.40 -10.39 -8.41 -8.88
Cytoplasm 3.96 16.62 25.88 13.62 16.23 0.65 21.39 26.82 23.02 -3.36 2.34 24.30 37.02 27.87 16.62
Cytoplasmic Foci -4.08 31.40 24.12 13.52 -7.01 4.84 23.89 22.87 13.92 -2.98 2.47 41.63 33.62 18.70 -12.02
Eisosomes -2.86 8.17 -3.31 0.68 -13.63 1.65 -8.90 -6.71 -7.70 5.19 0.19 6.61 -6.83 -6.24 -14.74
Endoplasmic Reticulum -0.92 0.01 -4.49 -3.35 -4.90 3.41 -5.49 -0.53 -6.44 6.09 3.29 -0.26 -2.98 -7.72 -3.83
Endosome -4.63 9.15 1.76 5.11 -4.52 -2.37 -1.80 1.90 3.36 3.12 -4.67 11.27 2.73 5.86 -4.62
Golgi -0.36 3.99 1.08 1.18 -1.86 -0.06 2.30 1.23 1.36 -1.85 0.22 4.71 1.83 1.49 -2.77
Lipid Particles -0.03 3.43 -1.97 -1.59 -6.36 2.33 -1.75 -1.25 -3.44 0.13 1.99 4.42 -2.18 -4.09 -7.19
Mitochondria 0.68 2.78 2.90 0.80 1.01 0.28 0.77 1.77 1.17 0.61 0.66 3.06 3.17 1.43 0.49
Mitotic Spindle -0.11 -3.97 -5.28 -4.97 -4.16 -3.55 -3.23 -6.07 -2.59 -4.83 -3.09 -5.60 -7.86 -4.81 -5.43
None 0.69 -11.26 -1.58 -1.77 3.56 2.32 -2.53 -3.92 -5.92 1.09 1.99 -10.36 -4.25 -5.44 3.27
Nuclear Periphery -0.32 -29.19 -12.30 -12.15 -2.29 -1.66 -15.80 -17.36 -13.79 5.92 -2.28 -32.68 -19.86 -17.21 0.93
Nuclear Periphery Foci -3.68 -1.38 -3.45 -0.16 -3.27 0.38 -9.01 -9.39 -8.65 5.70 -2.94 -6.48 -7.05 -4.44 -4.47
Nucleolus -1.89 3.17 0.77 2.20 -2.30 -0.92 1.72 2.01 1.36 2.78 -1.10 2.32 2.27 1.43 -0.26
Nucleus 10.94 -33.17 -4.66 -11.80 18.73 2.88 -9.14 -5.36 -7.17 6.15 4.90 -33.41 -7.75 -10.39 20.67
Peroxisomes -2.65 5.22 6.50 3.07 -0.81 -1.07 4.57 0.26 1.10 -2.39 -2.71 5.10 2.38 3.38 -2.01
Vacuole -6.57 -12.96 -27.09 -19.12 -22.61 -6.97 -17.17 -29.06 -24.44 -0.41 -8.87 -20.33 -39.34 -32.03 -26.32
Vacuole Periphery -1.27 2.42 0.28 1.14 -1.19 -0.34 2.24 -0.30 -0.07 -1.11 -0.74 3.29 -0.28 0.24 -1.49
Standard name
Human Ortholog
Description NADH diphosphatase (pyrophosphatase); hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Npy1

Npy1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Npy1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available