Standard name
Human Ortholog
Description Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions localization is regulated by Grx3p, Grx4p, and Fra2p, and promoter binding is negatively regulated via Grx3p-Grx4p binding; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.16 0.26 0.59 0.48 0.61 0.63 0.67 0.6 0.55 0.44 0.53 0.12 0.18 0.18 0.52 0.31 0.57 0.08 0.11 0.13 0.5 0.42 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0.2 0.14 0.26 0.3 0.48 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.63 0.54 0.64 0.18 0.21 0.15 0.11 0.09 0.06 0.12 0.12 0.17 0.85 0.76 0.76 0.14 0.22 0.08 0.7 0.67 0.51 0.24 0.27 0.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09
Vac/Vac Membrane 0.32 0.46 0.15 0.27 0.32 0.17 0.15 0.13 0.2 0.19 0.13 0.12 0 0.05 0.05 0.43 0.57 0.5 0.09 0.08 0.21 0.13 0.14 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 1
Bud 1 0 0 1 0 1 2 5 2 1 3 2 0 0 0 0 0 0 8 5 6 6 6 13
Bud Neck 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 1 0 1 1 6 13
Bud Site 0 0 0 0 0 0 1 1 0 0 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 2 2 0 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 43 35 50 124 129 184 233 242 155 116 139 166 27 59 59 45 27 42 30 25 30 133 124 109
Endoplasmic Reticulum 1 2 2 0 1 1 0 1 0 0 0 2 0 0 1 5 3 1 0 1 2 0 1 2
Endosome 5 2 1 2 11 6 5 7 3 4 0 1 2 5 6 1 1 0 11 6 2 4 4 14
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0 2 2 0 0 0
Mitochondria 11 2 0 5 7 43 74 49 68 64 153 91 0 0 0 0 0 1 1 2 4 2 5 8
Nucleus 202 117 122 38 55 46 41 33 16 25 39 53 198 251 251 12 19 6 264 163 119 64 79 109
Nuclear Periphery 0 0 1 0 0 0 0 1 1 0 1 0 0 0 0 1 0 0 0 1 1 0 0 2
Nucleolus 2 0 0 0 1 0 0 1 0 0 0 0 3 3 3 0 0 0 6 3 1 0 3 8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 0 0 1 6 1 1 0 1 2 3 1 0 0 0 13 5 6 11 14 31
Vac/Vac Membrane 103 100 28 57 85 52 57 46 51 40 42 39 9 17 17 37 50 37 34 19 49 35 40 46
Unique Cell Count 323 218 192 211 267 304 371 359 257 211 317 312 234 330 330 86 88 74 377 242 234 268 295 371
Labelled Cell Count 369 259 205 230 291 335 417 394 297 251 379 357 241 340 340 101 101 87 377 242 234 268 295 371


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 6.9 6.7 6.2 6.7 5.2 5.5 5.6 4.8 4.8 4.5 4.8 7.8 7.9 7.9 7.3 8.7 7.5 6.6 6.8 6.8
Std Deviation (1e-4) 0.7 1.0 1.1 1.3 1.8 1.1 2.1 1.2 1.4 1.1 1.4 1.0 1.2 1.4 1.4 1.4 1.8 1.3 1.3 1.3 1.3
Intensity Change (Log2) – – – -0.11 0.0 -0.38 -0.27 -0.25 -0.46 -0.48 -0.56 -0.48 0.23 0.25 0.25 0.13 0.37 0.17 -0.02 0.02 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6.6 4.8 7.5 8.3 9.3 7.2 5.9 4.0 6.0 -3.9 -2.2 -2.2 4.3 0.8 4.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.4 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.5 6.6 5.4 0 0 0 0 0 0 0 0 0 0
Nucleus -9.3 -9.3 -11.1 -13.0 -13.5 -13.0 -10.8 -12.0 -10.7 5.0 3.1 3.1 -7.6 -6.5 -8.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.1 4.2 0.7 0.2 -0.6 1.4 1.2 -0.4 -0.7 -3.9 -3.7 -3.7 5.2 7.3 6.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1671 2.9382 2.599 2.6024 2.0519 2.3422 2.5905 3.6096 3.3906 2.8659 2.832 3.1704 3.332 4.1428 3.4088 3.8534 3.3637 3.5425
Actin 0.0144 0.0003 0.0189 0.004 0.0031 0.0115 0.0144 0.0002 0.0021 0.0022 0.0052 0.0022 0.0002 0.0001 0.0026 0.0001 0.0001 0.0002
Bud 0.0009 0.0002 0.0002 0.0002 0.0024 0.0007 0.0011 0.0001 0.0008 0.0008 0.0025 0.0002 0.0015 0.0056 0.0001 0.0001 0.0001 0.0002
Bud Neck 0.0013 0.0005 0.0006 0.0014 0.003 0.0054 0.0069 0.0005 0.0072 0.0025 0.0122 0.0025 0.0006 0.0004 0.0005 0.0005 0.0011 0.0026
Bud Periphery 0.0014 0.0002 0.0003 0.0001 0.0034 0.0016 0.001 0 0.0013 0.0006 0.0034 0.0003 0.0002 0.0005 0 0.0001 0 0.0001
Bud Site 0.0026 0.0011 0.0038 0.001 0.0022 0.0018 0.0031 0.0012 0.0061 0.0008 0.0029 0.0006 0.0007 0.0019 0.0006 0.0001 0.0001 0.0002
Cell Periphery 0.0003 0.0001 0.0001 0 0.0001 0.0003 0.0002 0 0.0001 0 0.0002 0 0 0 0 0 0 0
Cytoplasm 0.0876 0.1055 0.0429 0.0335 0.1389 0.0855 0.1751 0.1623 0.0753 0.1491 0.1652 0.1074 0.1695 0.122 0.1017 0.0312 0.1152 0.1525
Cytoplasmic Foci 0.0163 0.0015 0.0174 0.0531 0.0026 0.0031 0.0183 0.0011 0.0041 0.0026 0.0186 0.0026 0.0022 0.0008 0.0017 0.001 0.0004 0.0022
Eisosomes 0.0002 0 0.0001 0 0 0.0001 0.0001 0 0.0001 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0053 0.0022 0.0008 0.0009 0.0045 0.0049 0.0065 0.0014 0.0018 0.0023 0.0054 0.0016 0.0018 0.0012 0.0013 0.0032 0.0012 0.0013
Endosome 0.029 0.0057 0.0125 0.0048 0.0295 0.0198 0.0194 0.0011 0.0094 0.0038 0.0249 0.0056 0.0025 0.0014 0.0024 0.006 0.0013 0.0023
Golgi 0.0061 0.0006 0.0035 0.0065 0.0025 0.0053 0.0038 0 0.0027 0.0002 0.0073 0.0015 0.0001 0 0.0006 0.0001 0 0
Lipid Particles 0.0125 0.0004 0.0039 0.0401 0.0007 0.0022 0.0142 0 0.0035 0 0.0269 0.0017 0.0001 0 0.0006 0.0003 0 0
Mitochondria 0.0098 0.0009 0.0074 0.002 0.034 0.0183 0.0042 0.0003 0.0015 0.0053 0.0447 0.0012 0.0004 0.0004 0.0003 0.0008 0.0003 0.0002
None 0.0117 0.0028 0.0084 0.0012 0.0021 0.0023 0.0117 0.0139 0.0215 0.0126 0.0298 0.0094 0.0145 0.0112 0.0101 0.0028 0.001 0.0074
Nuclear Periphery 0.0327 0.0154 0.0111 0.0119 0.0288 0.0305 0.0256 0.0112 0.0124 0.0112 0.0285 0.0104 0.0119 0.0098 0.0113 0.0114 0.0134 0.0083
Nucleolus 0.003 0.0017 0.0018 0.0018 0.0028 0.0045 0.0033 0.0012 0.0024 0.0015 0.0031 0.0027 0.0014 0.0013 0.001 0.0015 0.0009 0.0019
Nucleus 0.7278 0.8491 0.8517 0.7589 0.7139 0.7745 0.6504 0.7957 0.8242 0.7533 0.5769 0.8198 0.7795 0.8314 0.849 0.9254 0.8559 0.8028
Peroxisomes 0.0048 0.0001 0.0077 0.0677 0.0005 0.0009 0.0068 0 0.0026 0.0012 0.006 0.0007 0.0001 0 0.0011 0.0001 0 0.0001
Punctate Nuclear 0.0211 0.0049 0.0032 0.01 0.0128 0.0168 0.0297 0.0084 0.0174 0.0488 0.0261 0.0278 0.0108 0.0104 0.0139 0.0135 0.0079 0.0166
Vacuole 0.0088 0.0053 0.0025 0.0008 0.01 0.0073 0.0031 0.0009 0.0025 0.0009 0.0067 0.0013 0.0018 0.0012 0.0007 0.0014 0.0008 0.0011
Vacuole Periphery 0.0023 0.0014 0.0012 0.0002 0.0021 0.0027 0.001 0.0002 0.0009 0.0003 0.0035 0.0004 0.0003 0.0002 0.0002 0.0004 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.7508 16.6537 19.0663 32.3025 20.7035 20.7472 34.1582 35.7182 32.4586 39.9573
Translational Efficiency 0.782 1.1848 0.9643 0.5467 0.5545 1.2382 0.5814 0.561 0.6756 0.6991

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1522 1121 2094 1660 1498 2202 1465 2239 3020 3323 3559 3899

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 638.10 717.42 923.89 1001.13 711.56 759.78 881.13 925.60 674.54 745.49 906.29 957.76
Standard Deviation 77.93 128.74 110.80 135.18 91.48 101.14 117.91 121.77 92.52 113.01 115.71 133.00
Intensity Change Log 2 — 0.169035 0.533939 0.649775 — 0.094596 0.308369 0.379403 — 0.130269 0.419419 0.513558

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000086 0.000724 0.000360 0.000776 0.000127 0.000202 0.000276 0.000566 0.000107 0.000378 0.000325 0.000655
Bud Neck 0.002316 0.005635 0.011589 0.014414 0.002271 0.008769 0.004164 0.029939 0.002293 0.007712 0.008533 0.023329
Bud Site 0.001795 0.007882 0.004353 0.010153 0.000953 0.003218 0.002341 0.020568 0.001377 0.004791 0.003525 0.016134
Cell Periphery 0.000119 0.000243 0.000108 0.000155 0.000098 0.000089 0.000151 0.000169 0.000108 0.000141 0.000126 0.000163
Cytoplasm 0.185744* 0.261522* 0.047199 0.025696 0.112967* 0.149723* 0.099692 0.153716* 0.149645* 0.187438* 0.068807 0.099212
Cytoplasmic Foci 0.020750 0.016210 0.000361 0.001845 0.010293 0.010515 0.001903 0.001118 0.015563 0.012436 0.000996 0.001428
Eisosomes 0.000029 0.000033 0.000016 0.000020 0.000020 0.000020 0.000024 0.000018 0.000025 0.000024 0.000019 0.000019
Endoplasmic Reticulum 0.000813 0.003423 0.003775 0.005646 0.001567 0.002258 0.003586 0.003200 0.001187 0.002651 0.003697 0.004241
Endosome 0.001254 0.001572 0.000412 0.001945 0.000679 0.002549 0.000615 0.001570 0.000969 0.002219 0.000495 0.001730
Golgi 0.000387 0.000475 0.000038 0.005197 0.000337 0.000497 0.000047 0.000288 0.000362 0.000490 0.000042 0.002378
Lipid Particles 0.001691 0.001902 0.000210 0.000204 0.001310 0.001898 0.000493 0.000299 0.001502 0.001899 0.000327 0.000258
Mitochondria 0.002612 0.003015 0.001383 0.002064 0.002783 0.005645 0.000723 0.004948 0.002697 0.004758 0.001112 0.003720
Mitotic Spindle 0.001493 0.005746 0.002170 0.012000 0.002306 0.009881 0.001438 0.050388 0.001896 0.008486 0.001869 0.034044
None 0.027473 0.025147 0.004560 0.005106 0.012163 0.007802 0.009142 0.013639 0.019879 0.013653 0.006446 0.010006
Nuclear Periphery 0.000560 0.001076 0.001494 0.001566 0.000837 0.002664 0.001178 0.003697 0.000697 0.002128 0.001364 0.002790
Nuclear Periphery Foci 0.000436 0.001131 0.000158 0.000379 0.000445 0.000661 0.000300 0.001093 0.000440 0.000820 0.000217 0.000789
Nucleolus 0.006084 0.004905 0.000737 0.000613 0.003110 0.004068 0.000920 0.001318 0.004608 0.004351 0.000813 0.001018
Nucleus 0.740694* 0.645850* 0.915855* 0.903591* 0.843672* 0.779329* 0.862646* 0.694001* 0.791774* 0.734301* 0.893953* 0.783234*
Peroxisomes 0.001280 0.002198 0.000072 0.000134 0.000853 0.001929 0.000199 0.000962 0.001068 0.002019 0.000124 0.000610
Vacuole 0.003870 0.010734 0.005009 0.008071 0.002761 0.007541 0.010065 0.017094 0.003320 0.008618 0.007090 0.013253
Vacuole Periphery 0.000515 0.000576 0.000139 0.000422 0.000449 0.000742 0.000095 0.001411 0.000482 0.000686 0.000121 0.000989

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.71 -13.08 -13.13 -0.68 -5.96 -2.78 -10.53 -10.87 -7.46 -3.18 -5.57 -16.49 -17.13 -4.80 -6.20
Bud Neck -3.65 -9.21 -15.52 -10.22 -4.23 -8.73 -4.47 -15.48 -9.34 -12.33 -9.10 -10.38 -20.86 -12.79 -10.95
Bud Site -3.93 -1.78 -3.46 1.10 -2.04 -3.70 -2.99 -7.99 -5.57 -6.02 -4.95 -3.09 -8.27 -3.69 -5.85
Cell Periphery -4.41 2.22 -0.86 4.01 -2.83 1.03 -4.03 -1.54 -2.56 2.74 -3.01 -0.48 -1.45 2.08 -0.78
Cytoplasm -6.77 22.95 25.43 27.61 4.55 -5.33 4.95 0.04 6.39 -6.00 -6.06 20.85 16.16 23.27 -6.60
Cytoplasmic Foci 1.88 12.66 12.29 9.60 -1.10 -0.08 7.41 8.38 11.71 2.44 2.43 14.69 14.84 15.00 0.68
Eisosomes -1.94 3.59 -0.21 2.20 -6.20 -0.13 -6.21 -3.31 -3.41 4.21 -0.47 -0.54 -1.64 -1.23 -1.36
Endoplasmic Reticulum -9.07 -21.61 -21.41 -7.07 -5.39 -4.10 -14.38 -14.46 -8.02 4.30 -9.97 -24.97 -25.95 -11.43 -0.39
Endosome -1.33 3.25 1.14 2.79 -2.07 -4.12 -0.60 -1.50 3.70 -1.13 -3.90 2.84 0.51 4.30 -2.81
Golgi -0.60 3.24 -1.53 -1.42 -1.96 -0.78 1.80 1.19 2.85 -4.31 -0.98 3.29 -1.18 -0.83 -2.12
Lipid Particles -1.03 6.51 6.37 5.65 -0.70 -1.89 4.41 5.44 5.50 2.86 -1.89 8.05 8.29 7.65 0.76
Mitochondria -0.37 1.66 0.29 0.82 -5.59 -2.27 1.94 -0.92 2.36 -11.18 -2.50 2.43 -0.77 2.71 -11.72
Mitotic Spindle -2.29 -1.82 -5.70 -2.96 -4.90 -4.08 -0.76 -9.93 -6.63 -9.48 -4.98 -1.64 -11.56 -6.92 -10.86
None 1.16 10.92 9.97 8.25 -3.50 3.31 3.28 0.54 -4.36 -3.96 4.48 11.21 8.38 4.45 -6.62
Nuclear Periphery -7.15 -15.44 -15.86 -11.48 -4.49 -5.11 -8.20 -12.88 -3.04 -9.56 -6.06 -16.50 -18.20 -4.65 -10.08
Nuclear Periphery Foci -3.66 1.70 1.13 4.36 -0.47 -1.46 0.44 -0.87 0.42 -1.15 -3.26 1.76 -0.16 2.72 -1.43
Nucleolus 1.47 9.08 8.16 4.30 -1.07 -2.16 8.64 5.16 5.78 -4.25 0.58 11.76 9.52 7.24 -3.65
Nucleus 7.38 -21.76 -17.72 -23.37 4.58 7.53 -2.63 10.28 2.69 14.38 7.82 -18.29 -3.79 -12.48 17.09
Peroxisomes -1.37 2.49 2.45 3.98 -1.04 -2.60 2.72 2.10 4.80 -1.34 -2.52 3.53 3.09 6.34 -1.84
Vacuole -6.36 -5.29 -8.58 -2.05 -5.33 -5.98 -9.86 -16.47 -12.67 -5.51 -8.29 -11.26 -18.32 -11.92 -9.10
Vacuole Periphery -0.22 1.84 1.47 2.84 -1.61 -1.16 1.90 -0.25 1.04 -2.79 -1.08 2.62 0.65 2.09 -3.02
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions localization is regulated by Grx3p, Grx4p, and Fra2p, and promoter binding is negatively regulated via Grx3p-Grx4p binding; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages nucleus (84%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Aft1

Aft1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aft1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available