Standard name | |
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Human Ortholog | |
Description | Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions localization is regulated by Grx3p, Grx4p, and Fra2p, and promoter binding is negatively regulated via Grx3p-Grx4p binding; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 |
Bud | 1 | 0 | 0 | 1 | 0 | 1 | 2 | 5 | 2 | 1 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 5 | 6 | 6 | 6 | 13 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 6 | 13 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 43 | 35 | 50 | 124 | 129 | 184 | 233 | 242 | 155 | 116 | 139 | 166 | 27 | 59 | 59 | 45 | 27 | 42 | 30 | 25 | 30 | 133 | 124 | 109 |
Endoplasmic Reticulum | 1 | 2 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 5 | 3 | 1 | 0 | 1 | 2 | 0 | 1 | 2 |
Endosome | 5 | 2 | 1 | 2 | 11 | 6 | 5 | 7 | 3 | 4 | 0 | 1 | 2 | 5 | 6 | 1 | 1 | 0 | 11 | 6 | 2 | 4 | 4 | 14 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 0 |
Mitochondria | 11 | 2 | 0 | 5 | 7 | 43 | 74 | 49 | 68 | 64 | 153 | 91 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 4 | 2 | 5 | 8 |
Nucleus | 202 | 117 | 122 | 38 | 55 | 46 | 41 | 33 | 16 | 25 | 39 | 53 | 198 | 251 | 251 | 12 | 19 | 6 | 264 | 163 | 119 | 64 | 79 | 109 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 |
Nucleolus | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 3 | 3 | 0 | 0 | 0 | 6 | 3 | 1 | 0 | 3 | 8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 6 | 1 | 1 | 0 | 1 | 2 | 3 | 1 | 0 | 0 | 0 | 13 | 5 | 6 | 11 | 14 | 31 |
Vac/Vac Membrane | 103 | 100 | 28 | 57 | 85 | 52 | 57 | 46 | 51 | 40 | 42 | 39 | 9 | 17 | 17 | 37 | 50 | 37 | 34 | 19 | 49 | 35 | 40 | 46 |
Unique Cell Count | 323 | 218 | 192 | 211 | 267 | 304 | 371 | 359 | 257 | 211 | 317 | 312 | 234 | 330 | 330 | 86 | 88 | 74 | 377 | 242 | 234 | 268 | 295 | 371 |
Labelled Cell Count | 369 | 259 | 205 | 230 | 291 | 335 | 417 | 394 | 297 | 251 | 379 | 357 | 241 | 340 | 340 | 101 | 101 | 87 | 377 | 242 | 234 | 268 | 295 | 371 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 6.9 | 6.7 | 6.2 | 6.7 | 5.2 | 5.5 | 5.6 | 4.8 | 4.8 | 4.5 | 4.8 | 7.8 | 7.9 | 7.9 | 7.3 | 8.7 | 7.5 | 6.6 | 6.8 | 6.8 |
Std Deviation (1e-4) | 0.7 | 1.0 | 1.1 | 1.3 | 1.8 | 1.1 | 2.1 | 1.2 | 1.4 | 1.1 | 1.4 | 1.0 | 1.2 | 1.4 | 1.4 | 1.4 | 1.8 | 1.3 | 1.3 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.0 | -0.38 | -0.27 | -0.25 | -0.46 | -0.48 | -0.56 | -0.48 | 0.23 | 0.25 | 0.25 | 0.13 | 0.37 | 0.17 | -0.02 | 0.02 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6.6 | 4.8 | 7.5 | 8.3 | 9.3 | 7.2 | 5.9 | 4.0 | 6.0 | -3.9 | -2.2 | -2.2 | 4.3 | 0.8 | 4.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.5 | 6.6 | 5.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -9.3 | -9.3 | -11.1 | -13.0 | -13.5 | -13.0 | -10.8 | -12.0 | -10.7 | 5.0 | 3.1 | 3.1 | -7.6 | -6.5 | -8.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.1 | 4.2 | 0.7 | 0.2 | -0.6 | 1.4 | 1.2 | -0.4 | -0.7 | -3.9 | -3.7 | -3.7 | 5.2 | 7.3 | 6.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.1671 | 2.9382 | 2.599 | 2.6024 | 2.0519 | 2.3422 | 2.5905 | 3.6096 | 3.3906 | 2.8659 | 2.832 | 3.1704 | 3.332 | 4.1428 | 3.4088 | 3.8534 | 3.3637 | 3.5425 |
Actin | 0.0144 | 0.0003 | 0.0189 | 0.004 | 0.0031 | 0.0115 | 0.0144 | 0.0002 | 0.0021 | 0.0022 | 0.0052 | 0.0022 | 0.0002 | 0.0001 | 0.0026 | 0.0001 | 0.0001 | 0.0002 |
Bud | 0.0009 | 0.0002 | 0.0002 | 0.0002 | 0.0024 | 0.0007 | 0.0011 | 0.0001 | 0.0008 | 0.0008 | 0.0025 | 0.0002 | 0.0015 | 0.0056 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Bud Neck | 0.0013 | 0.0005 | 0.0006 | 0.0014 | 0.003 | 0.0054 | 0.0069 | 0.0005 | 0.0072 | 0.0025 | 0.0122 | 0.0025 | 0.0006 | 0.0004 | 0.0005 | 0.0005 | 0.0011 | 0.0026 |
Bud Periphery | 0.0014 | 0.0002 | 0.0003 | 0.0001 | 0.0034 | 0.0016 | 0.001 | 0 | 0.0013 | 0.0006 | 0.0034 | 0.0003 | 0.0002 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 |
Bud Site | 0.0026 | 0.0011 | 0.0038 | 0.001 | 0.0022 | 0.0018 | 0.0031 | 0.0012 | 0.0061 | 0.0008 | 0.0029 | 0.0006 | 0.0007 | 0.0019 | 0.0006 | 0.0001 | 0.0001 | 0.0002 |
Cell Periphery | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0876 | 0.1055 | 0.0429 | 0.0335 | 0.1389 | 0.0855 | 0.1751 | 0.1623 | 0.0753 | 0.1491 | 0.1652 | 0.1074 | 0.1695 | 0.122 | 0.1017 | 0.0312 | 0.1152 | 0.1525 |
Cytoplasmic Foci | 0.0163 | 0.0015 | 0.0174 | 0.0531 | 0.0026 | 0.0031 | 0.0183 | 0.0011 | 0.0041 | 0.0026 | 0.0186 | 0.0026 | 0.0022 | 0.0008 | 0.0017 | 0.001 | 0.0004 | 0.0022 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0053 | 0.0022 | 0.0008 | 0.0009 | 0.0045 | 0.0049 | 0.0065 | 0.0014 | 0.0018 | 0.0023 | 0.0054 | 0.0016 | 0.0018 | 0.0012 | 0.0013 | 0.0032 | 0.0012 | 0.0013 |
Endosome | 0.029 | 0.0057 | 0.0125 | 0.0048 | 0.0295 | 0.0198 | 0.0194 | 0.0011 | 0.0094 | 0.0038 | 0.0249 | 0.0056 | 0.0025 | 0.0014 | 0.0024 | 0.006 | 0.0013 | 0.0023 |
Golgi | 0.0061 | 0.0006 | 0.0035 | 0.0065 | 0.0025 | 0.0053 | 0.0038 | 0 | 0.0027 | 0.0002 | 0.0073 | 0.0015 | 0.0001 | 0 | 0.0006 | 0.0001 | 0 | 0 |
Lipid Particles | 0.0125 | 0.0004 | 0.0039 | 0.0401 | 0.0007 | 0.0022 | 0.0142 | 0 | 0.0035 | 0 | 0.0269 | 0.0017 | 0.0001 | 0 | 0.0006 | 0.0003 | 0 | 0 |
Mitochondria | 0.0098 | 0.0009 | 0.0074 | 0.002 | 0.034 | 0.0183 | 0.0042 | 0.0003 | 0.0015 | 0.0053 | 0.0447 | 0.0012 | 0.0004 | 0.0004 | 0.0003 | 0.0008 | 0.0003 | 0.0002 |
None | 0.0117 | 0.0028 | 0.0084 | 0.0012 | 0.0021 | 0.0023 | 0.0117 | 0.0139 | 0.0215 | 0.0126 | 0.0298 | 0.0094 | 0.0145 | 0.0112 | 0.0101 | 0.0028 | 0.001 | 0.0074 |
Nuclear Periphery | 0.0327 | 0.0154 | 0.0111 | 0.0119 | 0.0288 | 0.0305 | 0.0256 | 0.0112 | 0.0124 | 0.0112 | 0.0285 | 0.0104 | 0.0119 | 0.0098 | 0.0113 | 0.0114 | 0.0134 | 0.0083 |
Nucleolus | 0.003 | 0.0017 | 0.0018 | 0.0018 | 0.0028 | 0.0045 | 0.0033 | 0.0012 | 0.0024 | 0.0015 | 0.0031 | 0.0027 | 0.0014 | 0.0013 | 0.001 | 0.0015 | 0.0009 | 0.0019 |
Nucleus | 0.7278 | 0.8491 | 0.8517 | 0.7589 | 0.7139 | 0.7745 | 0.6504 | 0.7957 | 0.8242 | 0.7533 | 0.5769 | 0.8198 | 0.7795 | 0.8314 | 0.849 | 0.9254 | 0.8559 | 0.8028 |
Peroxisomes | 0.0048 | 0.0001 | 0.0077 | 0.0677 | 0.0005 | 0.0009 | 0.0068 | 0 | 0.0026 | 0.0012 | 0.006 | 0.0007 | 0.0001 | 0 | 0.0011 | 0.0001 | 0 | 0.0001 |
Punctate Nuclear | 0.0211 | 0.0049 | 0.0032 | 0.01 | 0.0128 | 0.0168 | 0.0297 | 0.0084 | 0.0174 | 0.0488 | 0.0261 | 0.0278 | 0.0108 | 0.0104 | 0.0139 | 0.0135 | 0.0079 | 0.0166 |
Vacuole | 0.0088 | 0.0053 | 0.0025 | 0.0008 | 0.01 | 0.0073 | 0.0031 | 0.0009 | 0.0025 | 0.0009 | 0.0067 | 0.0013 | 0.0018 | 0.0012 | 0.0007 | 0.0014 | 0.0008 | 0.0011 |
Vacuole Periphery | 0.0023 | 0.0014 | 0.0012 | 0.0002 | 0.0021 | 0.0027 | 0.001 | 0.0002 | 0.0009 | 0.0003 | 0.0035 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.7508 | 16.6537 | 19.0663 | 32.3025 | 20.7035 | 20.7472 | 34.1582 | 35.7182 | 32.4586 | 39.9573 |
Translational Efficiency | 0.782 | 1.1848 | 0.9643 | 0.5467 | 0.5545 | 1.2382 | 0.5814 | 0.561 | 0.6756 | 0.6991 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1522 | 1121 | 2094 | 1660 | 1498 | 2202 | 1465 | 2239 | 3020 | 3323 | 3559 | 3899 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 638.10 | 717.42 | 923.89 | 1001.13 | 711.56 | 759.78 | 881.13 | 925.60 | 674.54 | 745.49 | 906.29 | 957.76 |
Standard Deviation | 77.93 | 128.74 | 110.80 | 135.18 | 91.48 | 101.14 | 117.91 | 121.77 | 92.52 | 113.01 | 115.71 | 133.00 |
Intensity Change Log 2 | — | 0.169035 | 0.533939 | 0.649775 | — | 0.094596 | 0.308369 | 0.379403 | — | 0.130269 | 0.419419 | 0.513558 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000086 | 0.000724 | 0.000360 | 0.000776 | 0.000127 | 0.000202 | 0.000276 | 0.000566 | 0.000107 | 0.000378 | 0.000325 | 0.000655 |
Bud Neck | 0.002316 | 0.005635 | 0.011589 | 0.014414 | 0.002271 | 0.008769 | 0.004164 | 0.029939 | 0.002293 | 0.007712 | 0.008533 | 0.023329 |
Bud Site | 0.001795 | 0.007882 | 0.004353 | 0.010153 | 0.000953 | 0.003218 | 0.002341 | 0.020568 | 0.001377 | 0.004791 | 0.003525 | 0.016134 |
Cell Periphery | 0.000119 | 0.000243 | 0.000108 | 0.000155 | 0.000098 | 0.000089 | 0.000151 | 0.000169 | 0.000108 | 0.000141 | 0.000126 | 0.000163 |
Cytoplasm | 0.185744* | 0.261522* | 0.047199 | 0.025696 | 0.112967* | 0.149723* | 0.099692 | 0.153716* | 0.149645* | 0.187438* | 0.068807 | 0.099212 |
Cytoplasmic Foci | 0.020750 | 0.016210 | 0.000361 | 0.001845 | 0.010293 | 0.010515 | 0.001903 | 0.001118 | 0.015563 | 0.012436 | 0.000996 | 0.001428 |
Eisosomes | 0.000029 | 0.000033 | 0.000016 | 0.000020 | 0.000020 | 0.000020 | 0.000024 | 0.000018 | 0.000025 | 0.000024 | 0.000019 | 0.000019 |
Endoplasmic Reticulum | 0.000813 | 0.003423 | 0.003775 | 0.005646 | 0.001567 | 0.002258 | 0.003586 | 0.003200 | 0.001187 | 0.002651 | 0.003697 | 0.004241 |
Endosome | 0.001254 | 0.001572 | 0.000412 | 0.001945 | 0.000679 | 0.002549 | 0.000615 | 0.001570 | 0.000969 | 0.002219 | 0.000495 | 0.001730 |
Golgi | 0.000387 | 0.000475 | 0.000038 | 0.005197 | 0.000337 | 0.000497 | 0.000047 | 0.000288 | 0.000362 | 0.000490 | 0.000042 | 0.002378 |
Lipid Particles | 0.001691 | 0.001902 | 0.000210 | 0.000204 | 0.001310 | 0.001898 | 0.000493 | 0.000299 | 0.001502 | 0.001899 | 0.000327 | 0.000258 |
Mitochondria | 0.002612 | 0.003015 | 0.001383 | 0.002064 | 0.002783 | 0.005645 | 0.000723 | 0.004948 | 0.002697 | 0.004758 | 0.001112 | 0.003720 |
Mitotic Spindle | 0.001493 | 0.005746 | 0.002170 | 0.012000 | 0.002306 | 0.009881 | 0.001438 | 0.050388 | 0.001896 | 0.008486 | 0.001869 | 0.034044 |
None | 0.027473 | 0.025147 | 0.004560 | 0.005106 | 0.012163 | 0.007802 | 0.009142 | 0.013639 | 0.019879 | 0.013653 | 0.006446 | 0.010006 |
Nuclear Periphery | 0.000560 | 0.001076 | 0.001494 | 0.001566 | 0.000837 | 0.002664 | 0.001178 | 0.003697 | 0.000697 | 0.002128 | 0.001364 | 0.002790 |
Nuclear Periphery Foci | 0.000436 | 0.001131 | 0.000158 | 0.000379 | 0.000445 | 0.000661 | 0.000300 | 0.001093 | 0.000440 | 0.000820 | 0.000217 | 0.000789 |
Nucleolus | 0.006084 | 0.004905 | 0.000737 | 0.000613 | 0.003110 | 0.004068 | 0.000920 | 0.001318 | 0.004608 | 0.004351 | 0.000813 | 0.001018 |
Nucleus | 0.740694* | 0.645850* | 0.915855* | 0.903591* | 0.843672* | 0.779329* | 0.862646* | 0.694001* | 0.791774* | 0.734301* | 0.893953* | 0.783234* |
Peroxisomes | 0.001280 | 0.002198 | 0.000072 | 0.000134 | 0.000853 | 0.001929 | 0.000199 | 0.000962 | 0.001068 | 0.002019 | 0.000124 | 0.000610 |
Vacuole | 0.003870 | 0.010734 | 0.005009 | 0.008071 | 0.002761 | 0.007541 | 0.010065 | 0.017094 | 0.003320 | 0.008618 | 0.007090 | 0.013253 |
Vacuole Periphery | 0.000515 | 0.000576 | 0.000139 | 0.000422 | 0.000449 | 0.000742 | 0.000095 | 0.001411 | 0.000482 | 0.000686 | 0.000121 | 0.000989 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.71 | -13.08 | -13.13 | -0.68 | -5.96 | -2.78 | -10.53 | -10.87 | -7.46 | -3.18 | -5.57 | -16.49 | -17.13 | -4.80 | -6.20 |
Bud Neck | -3.65 | -9.21 | -15.52 | -10.22 | -4.23 | -8.73 | -4.47 | -15.48 | -9.34 | -12.33 | -9.10 | -10.38 | -20.86 | -12.79 | -10.95 |
Bud Site | -3.93 | -1.78 | -3.46 | 1.10 | -2.04 | -3.70 | -2.99 | -7.99 | -5.57 | -6.02 | -4.95 | -3.09 | -8.27 | -3.69 | -5.85 |
Cell Periphery | -4.41 | 2.22 | -0.86 | 4.01 | -2.83 | 1.03 | -4.03 | -1.54 | -2.56 | 2.74 | -3.01 | -0.48 | -1.45 | 2.08 | -0.78 |
Cytoplasm | -6.77 | 22.95 | 25.43 | 27.61 | 4.55 | -5.33 | 4.95 | 0.04 | 6.39 | -6.00 | -6.06 | 20.85 | 16.16 | 23.27 | -6.60 |
Cytoplasmic Foci | 1.88 | 12.66 | 12.29 | 9.60 | -1.10 | -0.08 | 7.41 | 8.38 | 11.71 | 2.44 | 2.43 | 14.69 | 14.84 | 15.00 | 0.68 |
Eisosomes | -1.94 | 3.59 | -0.21 | 2.20 | -6.20 | -0.13 | -6.21 | -3.31 | -3.41 | 4.21 | -0.47 | -0.54 | -1.64 | -1.23 | -1.36 |
Endoplasmic Reticulum | -9.07 | -21.61 | -21.41 | -7.07 | -5.39 | -4.10 | -14.38 | -14.46 | -8.02 | 4.30 | -9.97 | -24.97 | -25.95 | -11.43 | -0.39 |
Endosome | -1.33 | 3.25 | 1.14 | 2.79 | -2.07 | -4.12 | -0.60 | -1.50 | 3.70 | -1.13 | -3.90 | 2.84 | 0.51 | 4.30 | -2.81 |
Golgi | -0.60 | 3.24 | -1.53 | -1.42 | -1.96 | -0.78 | 1.80 | 1.19 | 2.85 | -4.31 | -0.98 | 3.29 | -1.18 | -0.83 | -2.12 |
Lipid Particles | -1.03 | 6.51 | 6.37 | 5.65 | -0.70 | -1.89 | 4.41 | 5.44 | 5.50 | 2.86 | -1.89 | 8.05 | 8.29 | 7.65 | 0.76 |
Mitochondria | -0.37 | 1.66 | 0.29 | 0.82 | -5.59 | -2.27 | 1.94 | -0.92 | 2.36 | -11.18 | -2.50 | 2.43 | -0.77 | 2.71 | -11.72 |
Mitotic Spindle | -2.29 | -1.82 | -5.70 | -2.96 | -4.90 | -4.08 | -0.76 | -9.93 | -6.63 | -9.48 | -4.98 | -1.64 | -11.56 | -6.92 | -10.86 |
None | 1.16 | 10.92 | 9.97 | 8.25 | -3.50 | 3.31 | 3.28 | 0.54 | -4.36 | -3.96 | 4.48 | 11.21 | 8.38 | 4.45 | -6.62 |
Nuclear Periphery | -7.15 | -15.44 | -15.86 | -11.48 | -4.49 | -5.11 | -8.20 | -12.88 | -3.04 | -9.56 | -6.06 | -16.50 | -18.20 | -4.65 | -10.08 |
Nuclear Periphery Foci | -3.66 | 1.70 | 1.13 | 4.36 | -0.47 | -1.46 | 0.44 | -0.87 | 0.42 | -1.15 | -3.26 | 1.76 | -0.16 | 2.72 | -1.43 |
Nucleolus | 1.47 | 9.08 | 8.16 | 4.30 | -1.07 | -2.16 | 8.64 | 5.16 | 5.78 | -4.25 | 0.58 | 11.76 | 9.52 | 7.24 | -3.65 |
Nucleus | 7.38 | -21.76 | -17.72 | -23.37 | 4.58 | 7.53 | -2.63 | 10.28 | 2.69 | 14.38 | 7.82 | -18.29 | -3.79 | -12.48 | 17.09 |
Peroxisomes | -1.37 | 2.49 | 2.45 | 3.98 | -1.04 | -2.60 | 2.72 | 2.10 | 4.80 | -1.34 | -2.52 | 3.53 | 3.09 | 6.34 | -1.84 |
Vacuole | -6.36 | -5.29 | -8.58 | -2.05 | -5.33 | -5.98 | -9.86 | -16.47 | -12.67 | -5.51 | -8.29 | -11.26 | -18.32 | -11.92 | -9.10 |
Vacuole Periphery | -0.22 | 1.84 | 1.47 | 2.84 | -1.61 | -1.16 | 1.90 | -0.25 | 1.04 | -2.79 | -1.08 | 2.62 | 0.65 | 2.09 | -3.02 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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