Standard name | |
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Human Ortholog | |
Description | Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions localization is regulated by Grx3p, Grx4p, and Fra2p, and promoter binding is negatively regulated via Grx3p-Grx4p binding; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 |
Bud | 1 | 0 | 0 | 1 | 0 | 1 | 2 | 5 | 2 | 1 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 5 | 6 | 6 | 6 | 13 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 6 | 13 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 43 | 35 | 50 | 124 | 129 | 184 | 233 | 242 | 155 | 116 | 139 | 166 | 27 | 59 | 59 | 45 | 27 | 42 | 30 | 25 | 30 | 133 | 124 | 109 |
Endoplasmic Reticulum | 1 | 2 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 5 | 3 | 1 | 0 | 1 | 2 | 0 | 1 | 2 |
Endosome | 5 | 2 | 1 | 2 | 11 | 6 | 5 | 7 | 3 | 4 | 0 | 1 | 2 | 5 | 6 | 1 | 1 | 0 | 11 | 6 | 2 | 4 | 4 | 14 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 0 |
Mitochondria | 11 | 2 | 0 | 5 | 7 | 43 | 74 | 49 | 68 | 64 | 153 | 91 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 4 | 2 | 5 | 8 |
Nucleus | 202 | 117 | 122 | 38 | 55 | 46 | 41 | 33 | 16 | 25 | 39 | 53 | 198 | 251 | 251 | 12 | 19 | 6 | 264 | 163 | 119 | 64 | 79 | 109 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 |
Nucleolus | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 3 | 3 | 0 | 0 | 0 | 6 | 3 | 1 | 0 | 3 | 8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 6 | 1 | 1 | 0 | 1 | 2 | 3 | 1 | 0 | 0 | 0 | 13 | 5 | 6 | 11 | 14 | 31 |
Vac/Vac Membrane | 103 | 100 | 28 | 57 | 85 | 52 | 57 | 46 | 51 | 40 | 42 | 39 | 9 | 17 | 17 | 37 | 50 | 37 | 34 | 19 | 49 | 35 | 40 | 46 |
Unique Cell Count | 323 | 218 | 192 | 211 | 267 | 304 | 371 | 359 | 257 | 211 | 317 | 312 | 234 | 330 | 330 | 86 | 88 | 74 | 377 | 242 | 234 | 268 | 295 | 371 |
Labelled Cell Count | 369 | 259 | 205 | 230 | 291 | 335 | 417 | 394 | 297 | 251 | 379 | 357 | 241 | 340 | 340 | 101 | 101 | 87 | 377 | 242 | 234 | 268 | 295 | 371 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 6.9 | 6.7 | 6.2 | 6.7 | 5.2 | 5.5 | 5.6 | 4.8 | 4.8 | 4.5 | 4.8 | 7.8 | 7.9 | 7.9 | 7.3 | 8.7 | 7.5 | 6.6 | 6.8 | 6.8 |
Std Deviation (1e-4) | 0.7 | 1.0 | 1.1 | 1.3 | 1.8 | 1.1 | 2.1 | 1.2 | 1.4 | 1.1 | 1.4 | 1.0 | 1.2 | 1.4 | 1.4 | 1.4 | 1.8 | 1.3 | 1.3 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.0 | -0.38 | -0.27 | -0.25 | -0.46 | -0.48 | -0.56 | -0.48 | 0.23 | 0.25 | 0.25 | 0.13 | 0.37 | 0.17 | -0.02 | 0.02 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6.6 | 4.8 | 7.5 | 8.3 | 9.3 | 7.2 | 5.9 | 4.0 | 6.0 | -3.9 | -2.2 | -2.2 | 4.3 | 0.8 | 4.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.5 | 6.6 | 5.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -9.3 | -9.3 | -11.1 | -13.0 | -13.5 | -13.0 | -10.8 | -12.0 | -10.7 | 5.0 | 3.1 | 3.1 | -7.6 | -6.5 | -8.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.1 | 4.2 | 0.7 | 0.2 | -0.6 | 1.4 | 1.2 | -0.4 | -0.7 | -3.9 | -3.7 | -3.7 | 5.2 | 7.3 | 6.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.1671 | 2.9382 | 2.599 | 2.6024 | 2.0519 | 2.3422 | 2.5905 | 3.6096 | 3.3906 | 2.8659 | 2.832 | 3.1704 | 3.332 | 4.1428 | 3.4088 | 3.8534 | 3.3637 | 3.5425 |
Actin | 0.0144 | 0.0003 | 0.0189 | 0.004 | 0.0031 | 0.0115 | 0.0144 | 0.0002 | 0.0021 | 0.0022 | 0.0052 | 0.0022 | 0.0002 | 0.0001 | 0.0026 | 0.0001 | 0.0001 | 0.0002 |
Bud | 0.0009 | 0.0002 | 0.0002 | 0.0002 | 0.0024 | 0.0007 | 0.0011 | 0.0001 | 0.0008 | 0.0008 | 0.0025 | 0.0002 | 0.0015 | 0.0056 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Bud Neck | 0.0013 | 0.0005 | 0.0006 | 0.0014 | 0.003 | 0.0054 | 0.0069 | 0.0005 | 0.0072 | 0.0025 | 0.0122 | 0.0025 | 0.0006 | 0.0004 | 0.0005 | 0.0005 | 0.0011 | 0.0026 |
Bud Periphery | 0.0014 | 0.0002 | 0.0003 | 0.0001 | 0.0034 | 0.0016 | 0.001 | 0 | 0.0013 | 0.0006 | 0.0034 | 0.0003 | 0.0002 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 |
Bud Site | 0.0026 | 0.0011 | 0.0038 | 0.001 | 0.0022 | 0.0018 | 0.0031 | 0.0012 | 0.0061 | 0.0008 | 0.0029 | 0.0006 | 0.0007 | 0.0019 | 0.0006 | 0.0001 | 0.0001 | 0.0002 |
Cell Periphery | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0876 | 0.1055 | 0.0429 | 0.0335 | 0.1389 | 0.0855 | 0.1751 | 0.1623 | 0.0753 | 0.1491 | 0.1652 | 0.1074 | 0.1695 | 0.122 | 0.1017 | 0.0312 | 0.1152 | 0.1525 |
Cytoplasmic Foci | 0.0163 | 0.0015 | 0.0174 | 0.0531 | 0.0026 | 0.0031 | 0.0183 | 0.0011 | 0.0041 | 0.0026 | 0.0186 | 0.0026 | 0.0022 | 0.0008 | 0.0017 | 0.001 | 0.0004 | 0.0022 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0053 | 0.0022 | 0.0008 | 0.0009 | 0.0045 | 0.0049 | 0.0065 | 0.0014 | 0.0018 | 0.0023 | 0.0054 | 0.0016 | 0.0018 | 0.0012 | 0.0013 | 0.0032 | 0.0012 | 0.0013 |
Endosome | 0.029 | 0.0057 | 0.0125 | 0.0048 | 0.0295 | 0.0198 | 0.0194 | 0.0011 | 0.0094 | 0.0038 | 0.0249 | 0.0056 | 0.0025 | 0.0014 | 0.0024 | 0.006 | 0.0013 | 0.0023 |
Golgi | 0.0061 | 0.0006 | 0.0035 | 0.0065 | 0.0025 | 0.0053 | 0.0038 | 0 | 0.0027 | 0.0002 | 0.0073 | 0.0015 | 0.0001 | 0 | 0.0006 | 0.0001 | 0 | 0 |
Lipid Particles | 0.0125 | 0.0004 | 0.0039 | 0.0401 | 0.0007 | 0.0022 | 0.0142 | 0 | 0.0035 | 0 | 0.0269 | 0.0017 | 0.0001 | 0 | 0.0006 | 0.0003 | 0 | 0 |
Mitochondria | 0.0098 | 0.0009 | 0.0074 | 0.002 | 0.034 | 0.0183 | 0.0042 | 0.0003 | 0.0015 | 0.0053 | 0.0447 | 0.0012 | 0.0004 | 0.0004 | 0.0003 | 0.0008 | 0.0003 | 0.0002 |
None | 0.0117 | 0.0028 | 0.0084 | 0.0012 | 0.0021 | 0.0023 | 0.0117 | 0.0139 | 0.0215 | 0.0126 | 0.0298 | 0.0094 | 0.0145 | 0.0112 | 0.0101 | 0.0028 | 0.001 | 0.0074 |
Nuclear Periphery | 0.0327 | 0.0154 | 0.0111 | 0.0119 | 0.0288 | 0.0305 | 0.0256 | 0.0112 | 0.0124 | 0.0112 | 0.0285 | 0.0104 | 0.0119 | 0.0098 | 0.0113 | 0.0114 | 0.0134 | 0.0083 |
Nucleolus | 0.003 | 0.0017 | 0.0018 | 0.0018 | 0.0028 | 0.0045 | 0.0033 | 0.0012 | 0.0024 | 0.0015 | 0.0031 | 0.0027 | 0.0014 | 0.0013 | 0.001 | 0.0015 | 0.0009 | 0.0019 |
Nucleus | 0.7278 | 0.8491 | 0.8517 | 0.7589 | 0.7139 | 0.7745 | 0.6504 | 0.7957 | 0.8242 | 0.7533 | 0.5769 | 0.8198 | 0.7795 | 0.8314 | 0.849 | 0.9254 | 0.8559 | 0.8028 |
Peroxisomes | 0.0048 | 0.0001 | 0.0077 | 0.0677 | 0.0005 | 0.0009 | 0.0068 | 0 | 0.0026 | 0.0012 | 0.006 | 0.0007 | 0.0001 | 0 | 0.0011 | 0.0001 | 0 | 0.0001 |
Punctate Nuclear | 0.0211 | 0.0049 | 0.0032 | 0.01 | 0.0128 | 0.0168 | 0.0297 | 0.0084 | 0.0174 | 0.0488 | 0.0261 | 0.0278 | 0.0108 | 0.0104 | 0.0139 | 0.0135 | 0.0079 | 0.0166 |
Vacuole | 0.0088 | 0.0053 | 0.0025 | 0.0008 | 0.01 | 0.0073 | 0.0031 | 0.0009 | 0.0025 | 0.0009 | 0.0067 | 0.0013 | 0.0018 | 0.0012 | 0.0007 | 0.0014 | 0.0008 | 0.0011 |
Vacuole Periphery | 0.0023 | 0.0014 | 0.0012 | 0.0002 | 0.0021 | 0.0027 | 0.001 | 0.0002 | 0.0009 | 0.0003 | 0.0035 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.7508 | 16.6537 | 19.0663 | 32.3025 | 20.7035 | 20.7472 | 34.1582 | 35.7182 | 32.4586 | 39.9573 |
Translational Efficiency | 0.782 | 1.1848 | 0.9643 | 0.5467 | 0.5545 | 1.2382 | 0.5814 | 0.561 | 0.6756 | 0.6991 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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