Standard name
Human Ortholog
Description Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions localization is regulated by Grx3p, Grx4p, and Fra2p, and promoter binding is negatively regulated via Grx3p-Grx4p binding; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.16 0.26 0.59 0.48 0.61 0.63 0.67 0.6 0.55 0.44 0.53 0.12 0.18 0.18 0.52 0.31 0.57 0.08 0.11 0.13 0.5 0.42 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0.2 0.14 0.26 0.3 0.48 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.63 0.54 0.64 0.18 0.21 0.15 0.11 0.09 0.06 0.12 0.12 0.17 0.85 0.76 0.76 0.14 0.22 0.08 0.7 0.67 0.51 0.24 0.27 0.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09
Vac/Vac Membrane 0.32 0.46 0.15 0.27 0.32 0.17 0.15 0.13 0.2 0.19 0.13 0.12 0 0.05 0.05 0.43 0.57 0.5 0.09 0.08 0.21 0.13 0.14 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 1
Bud 1 0 0 1 0 1 2 5 2 1 3 2 0 0 0 0 0 0 8 5 6 6 6 13
Bud Neck 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 1 0 1 1 6 13
Bud Site 0 0 0 0 0 0 1 1 0 0 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 2 2 0 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 43 35 50 124 129 184 233 242 155 116 139 166 27 59 59 45 27 42 30 25 30 133 124 109
Endoplasmic Reticulum 1 2 2 0 1 1 0 1 0 0 0 2 0 0 1 5 3 1 0 1 2 0 1 2
Endosome 5 2 1 2 11 6 5 7 3 4 0 1 2 5 6 1 1 0 11 6 2 4 4 14
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0 2 2 0 0 0
Mitochondria 11 2 0 5 7 43 74 49 68 64 153 91 0 0 0 0 0 1 1 2 4 2 5 8
Nucleus 202 117 122 38 55 46 41 33 16 25 39 53 198 251 251 12 19 6 264 163 119 64 79 109
Nuclear Periphery 0 0 1 0 0 0 0 1 1 0 1 0 0 0 0 1 0 0 0 1 1 0 0 2
Nucleolus 2 0 0 0 1 0 0 1 0 0 0 0 3 3 3 0 0 0 6 3 1 0 3 8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 0 0 1 6 1 1 0 1 2 3 1 0 0 0 13 5 6 11 14 31
Vac/Vac Membrane 103 100 28 57 85 52 57 46 51 40 42 39 9 17 17 37 50 37 34 19 49 35 40 46
Unique Cell Count 323 218 192 211 267 304 371 359 257 211 317 312 234 330 330 86 88 74 377 242 234 268 295 371
Labelled Cell Count 369 259 205 230 291 335 417 394 297 251 379 357 241 340 340 101 101 87 377 242 234 268 295 371


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 6.9 6.7 6.2 6.7 5.2 5.5 5.6 4.8 4.8 4.5 4.8 7.8 7.9 7.9 7.3 8.7 7.5 6.6 6.8 6.8
Std Deviation (1e-4) 0.7 1.0 1.1 1.3 1.8 1.1 2.1 1.2 1.4 1.1 1.4 1.0 1.2 1.4 1.4 1.4 1.8 1.3 1.3 1.3 1.3
Intensity Change (Log2) – – – -0.11 0.0 -0.38 -0.27 -0.25 -0.46 -0.48 -0.56 -0.48 0.23 0.25 0.25 0.13 0.37 0.17 -0.02 0.02 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6.6 4.8 7.5 8.3 9.3 7.2 5.9 4.0 6.0 -3.9 -2.2 -2.2 4.3 0.8 4.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.4 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.5 6.6 5.4 0 0 0 0 0 0 0 0 0 0
Nucleus -9.3 -9.3 -11.1 -13.0 -13.5 -13.0 -10.8 -12.0 -10.7 5.0 3.1 3.1 -7.6 -6.5 -8.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.1 4.2 0.7 0.2 -0.6 1.4 1.2 -0.4 -0.7 -3.9 -3.7 -3.7 5.2 7.3 6.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1671 2.9382 2.599 2.6024 2.0519 2.3422 2.5905 3.6096 3.3906 2.8659 2.832 3.1704 3.332 4.1428 3.4088 3.8534 3.3637 3.5425
Actin 0.0144 0.0003 0.0189 0.004 0.0031 0.0115 0.0144 0.0002 0.0021 0.0022 0.0052 0.0022 0.0002 0.0001 0.0026 0.0001 0.0001 0.0002
Bud 0.0009 0.0002 0.0002 0.0002 0.0024 0.0007 0.0011 0.0001 0.0008 0.0008 0.0025 0.0002 0.0015 0.0056 0.0001 0.0001 0.0001 0.0002
Bud Neck 0.0013 0.0005 0.0006 0.0014 0.003 0.0054 0.0069 0.0005 0.0072 0.0025 0.0122 0.0025 0.0006 0.0004 0.0005 0.0005 0.0011 0.0026
Bud Periphery 0.0014 0.0002 0.0003 0.0001 0.0034 0.0016 0.001 0 0.0013 0.0006 0.0034 0.0003 0.0002 0.0005 0 0.0001 0 0.0001
Bud Site 0.0026 0.0011 0.0038 0.001 0.0022 0.0018 0.0031 0.0012 0.0061 0.0008 0.0029 0.0006 0.0007 0.0019 0.0006 0.0001 0.0001 0.0002
Cell Periphery 0.0003 0.0001 0.0001 0 0.0001 0.0003 0.0002 0 0.0001 0 0.0002 0 0 0 0 0 0 0
Cytoplasm 0.0876 0.1055 0.0429 0.0335 0.1389 0.0855 0.1751 0.1623 0.0753 0.1491 0.1652 0.1074 0.1695 0.122 0.1017 0.0312 0.1152 0.1525
Cytoplasmic Foci 0.0163 0.0015 0.0174 0.0531 0.0026 0.0031 0.0183 0.0011 0.0041 0.0026 0.0186 0.0026 0.0022 0.0008 0.0017 0.001 0.0004 0.0022
Eisosomes 0.0002 0 0.0001 0 0 0.0001 0.0001 0 0.0001 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0053 0.0022 0.0008 0.0009 0.0045 0.0049 0.0065 0.0014 0.0018 0.0023 0.0054 0.0016 0.0018 0.0012 0.0013 0.0032 0.0012 0.0013
Endosome 0.029 0.0057 0.0125 0.0048 0.0295 0.0198 0.0194 0.0011 0.0094 0.0038 0.0249 0.0056 0.0025 0.0014 0.0024 0.006 0.0013 0.0023
Golgi 0.0061 0.0006 0.0035 0.0065 0.0025 0.0053 0.0038 0 0.0027 0.0002 0.0073 0.0015 0.0001 0 0.0006 0.0001 0 0
Lipid Particles 0.0125 0.0004 0.0039 0.0401 0.0007 0.0022 0.0142 0 0.0035 0 0.0269 0.0017 0.0001 0 0.0006 0.0003 0 0
Mitochondria 0.0098 0.0009 0.0074 0.002 0.034 0.0183 0.0042 0.0003 0.0015 0.0053 0.0447 0.0012 0.0004 0.0004 0.0003 0.0008 0.0003 0.0002
None 0.0117 0.0028 0.0084 0.0012 0.0021 0.0023 0.0117 0.0139 0.0215 0.0126 0.0298 0.0094 0.0145 0.0112 0.0101 0.0028 0.001 0.0074
Nuclear Periphery 0.0327 0.0154 0.0111 0.0119 0.0288 0.0305 0.0256 0.0112 0.0124 0.0112 0.0285 0.0104 0.0119 0.0098 0.0113 0.0114 0.0134 0.0083
Nucleolus 0.003 0.0017 0.0018 0.0018 0.0028 0.0045 0.0033 0.0012 0.0024 0.0015 0.0031 0.0027 0.0014 0.0013 0.001 0.0015 0.0009 0.0019
Nucleus 0.7278 0.8491 0.8517 0.7589 0.7139 0.7745 0.6504 0.7957 0.8242 0.7533 0.5769 0.8198 0.7795 0.8314 0.849 0.9254 0.8559 0.8028
Peroxisomes 0.0048 0.0001 0.0077 0.0677 0.0005 0.0009 0.0068 0 0.0026 0.0012 0.006 0.0007 0.0001 0 0.0011 0.0001 0 0.0001
Punctate Nuclear 0.0211 0.0049 0.0032 0.01 0.0128 0.0168 0.0297 0.0084 0.0174 0.0488 0.0261 0.0278 0.0108 0.0104 0.0139 0.0135 0.0079 0.0166
Vacuole 0.0088 0.0053 0.0025 0.0008 0.01 0.0073 0.0031 0.0009 0.0025 0.0009 0.0067 0.0013 0.0018 0.0012 0.0007 0.0014 0.0008 0.0011
Vacuole Periphery 0.0023 0.0014 0.0012 0.0002 0.0021 0.0027 0.001 0.0002 0.0009 0.0003 0.0035 0.0004 0.0003 0.0002 0.0002 0.0004 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.7508 16.6537 19.0663 32.3025 20.7035 20.7472 34.1582 35.7182 32.4586 39.9573
Translational Efficiency 0.782 1.1848 0.9643 0.5467 0.5545 1.2382 0.5814 0.561 0.6756 0.6991

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions localization is regulated by Grx3p, Grx4p, and Fra2p, and promoter binding is negatively regulated via Grx3p-Grx4p binding; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages nucleus (84%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Aft1

Aft1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aft1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available