Standard name
Human Ortholog
Description Subunit of the BLOC-1 complex involved in endosomal maturation; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.62 0.58 0.58 0.71 0.71 0.8 0.84 0.85 0.85 0.81 0.83 0.53 0.43 0.51 0.78 0.81 0.8 0.18 0.22 0.28 0.21 0.18 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0.25 0.31 0.24 0.22 0.19 0.07 0.09 0 0 0 0 0.37 0.51 0.47 0.13 0.11 0.14 0.58 0.57 0.46 0.58 0.64 0.67
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.19 0.05 0.2 0.14 0.26 0.26 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0.06 0.21 0.09 0.09 0 0.06 0 0 0 0 0.07 0.13 0.11 0.06 0 0 0.08 0.06 0.11 0.06 0 0.07
Vac/Vac Membrane 0.12 0.15 0.08 0 0.1 0 0.06 0 0 0 0 0.12 0.1 0.12 0.08 0.06 0.06 0.06 0.09 0.06 0.08 0.08 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 5 0 2 1 0 0 1 1 0 3 3 1 0 0 1 0 3 2 0 2 2
Bud 0 1 5 3 2 13 4 5 10 13 6 2 4 2 1 0 1 0 2 1 0 3 5
Bud Neck 0 0 1 1 0 2 1 2 1 0 0 0 0 1 0 2 0 0 1 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1 1 1 2 3 4 4 4 7 1 0 0 1 1 2 0 0 0 0 0 0
Cytoplasm 10 202 156 198 245 314 483 434 484 417 490 103 149 185 183 199 170 13 97 86 62 58 50
Endoplasmic Reticulum 0 9 1 1 0 0 0 1 0 2 0 7 4 2 14 11 9 0 0 2 2 1 3
Endosome 4 109 65 62 67 27 49 18 22 16 16 73 176 171 31 27 30 43 251 141 170 213 277
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 2 2 1 0 2
Mitochondria 0 6 0 4 6 74 30 102 82 132 152 0 1 0 8 3 1 4 14 13 9 4 8
Nucleus 0 0 0 1 0 2 0 2 4 3 0 0 0 0 1 2 1 0 1 0 2 0 1
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 1
Nucleolus 1 1 0 0 0 0 0 1 1 0 0 0 2 2 0 1 1 0 1 0 1 1 0
Peroxisomes 0 0 3 1 3 0 3 1 0 1 1 0 7 4 0 0 0 0 0 0 0 1 0
SpindlePole 1 22 56 25 30 6 33 5 14 5 5 13 46 39 15 9 6 6 25 33 17 13 27
Vac/Vac Membrane 2 52 22 8 36 13 37 13 12 12 10 24 33 44 18 15 12 4 37 17 25 28 25
Unique Cell Count 16 349 268 279 347 394 573 512 567 517 592 195 347 366 235 246 212 76 443 306 297 336 414
Labelled Cell Count 18 406 315 305 392 454 643 588 635 606 687 227 425 451 273 271 235 76 443 306 297 336 414


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 5.3 6.2 5.8 6.4 4.6 5.6 4.8 5.2 4.9 4.9 5.9 6.4 6.6 6.2 6.4 6.0 5.2 5.4 5.8
Std Deviation (1e-4) 0.6 0.7 1.6 1.2 1.6 1.6 1.5 1.3 1.2 0.9 1.0 1.1 1.5 1.4 1.3 1.1 1.3 1.4 1.2 1.7
Intensity Change (Log2) -0.09 0.05 -0.42 -0.14 -0.37 -0.25 -0.35 -0.34 -0.06 0.05 0.08 0.01 0.05 -0.05 -0.24 -0.19 -0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.1 3.2 6.0 8.3 8.2 8.7 6.7 7.7 -1.2 -3.8 -1.9 4.7 5.6 5.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 3.7 3.1 0
Endosome -0.6 -1.5 0 -6.2 0 -9.0 0 -10.0 3.1 6.7 5.8 -3.1 -3.9 -2.8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.8 0 6.6 0 9.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -3.9 -4.3 0 -6.6 0 -9.0 0 -10.6 -4.2 -2.5 -3.6 -4.7 -5.9 -5.9
Vacuole -2.7 0.9 -2.8 -0.9 -3.6 -4.2 -3.8 -4.7 1.5 0.6 1.6 -0.2 -0.9 -1.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2496 0.0067 -0.413 -0.7569 -0.0602 -0.2468 1.1593 1.8654 1.4394 1.0424 1.0618 1.4829 3.5143 4.5449 4.0825 4.0584 4.1329 4.4453
Actin 0.016 0.0001 0.0092 0.0036 0.0108 0.0008 0.0163 0.003 0.0039 0.0004 0.0002 0.0004 0.0011 0.0001 0.0003 0.0001 0.0012 0.0005
Bud 0.0029 0.0011 0.0026 0.0001 0.0004 0.0001 0.0002 0.002 0.0036 0.0002 0.0002 0.0001 0.0005 0.0003 0.0004 0.0001 0.0001 0
Bud Neck 0.0032 0.0003 0.0009 0.0003 0.0005 0.0007 0.0049 0.0003 0.0006 0.0002 0.0002 0.0007 0.0002 0.0003 0.0003 0.0001 0.0004 0.0005
Bud Periphery 0.0016 0.0002 0.001 0.0001 0.0006 0.0001 0.0003 0.0011 0.0041 0.0002 0.0002 0.0001 0.0009 0.0001 0.0009 0 0.0001 0
Bud Site 0.0017 0.0113 0.0046 0.0004 0.0008 0.0002 0.0011 0.0037 0.0033 0.0003 0.0001 0.0003 0.0016 0.0009 0.001 0.0001 0.0003 0.0002
Cell Periphery 0.0002 0.0003 0.0002 0 0.0001 0 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0.0001 0 0 0.0001
Cytoplasm 0.049 0.0491 0.076 0.0173 0.0449 0.0786 0.0777 0.1062 0.1041 0.0332 0.0598 0.1042 0.1015 0.1479 0.0971 0.0794 0.0569 0.064
Cytoplasmic Foci 0.274 0.3775 0.3171 0.2828 0.2737 0.3496 0.2818 0.3172 0.2916 0.2792 0.2816 0.3789 0.2892 0.2887 0.2894 0.3255 0.3615 0.3518
Eisosomes 0.0003 0 0.0001 0.0001 0.0002 0 0.0001 0.0001 0.0001 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.004 0.0011 0.0032 0.0007 0.0022 0.0022 0.0045 0.0023 0.0035 0.0011 0.0013 0.0018 0.0033 0.0019 0.0013 0.0005 0.001 0.0015
Endosome 0.4058 0.3718 0.3544 0.4796 0.3391 0.3702 0.3923 0.3747 0.4115 0.5135 0.4964 0.3362 0.4538 0.4202 0.4031 0.528 0.4414 0.424
Golgi 0.0234 0.0118 0.0178 0.0292 0.0617 0.0122 0.0171 0.0152 0.0216 0.0369 0.0198 0.0169 0.0137 0.0145 0.019 0.0218 0.0294 0.016
Lipid Particles 0.0554 0.0518 0.0471 0.0403 0.0809 0.0569 0.0421 0.0385 0.0362 0.0311 0.0201 0.0315 0.0388 0.0202 0.0236 0.006 0.0287 0.0408
Mitochondria 0.0171 0.0009 0.0055 0.0193 0.0513 0.0012 0.0022 0.0014 0.0022 0.0129 0.0018 0.004 0.0011 0.0009 0.0028 0.0018 0.0065 0.0013
None 0.0723 0.0873 0.0881 0.0665 0.0617 0.0704 0.0742 0.0756 0.0724 0.0454 0.0576 0.0648 0.0613 0.0596 0.1019 0.0033 0.0264 0.0568
Nuclear Periphery 0.0094 0.0008 0.0031 0.0005 0.0029 0.0048 0.0048 0.002 0.0017 0.001 0.0007 0.0013 0.0013 0.0011 0.0008 0.0002 0.0004 0.0005
Nucleolus 0.0011 0.0007 0.001 0.0002 0.0003 0.0007 0.0228 0.0214 0.0003 0.0002 0.0001 0.0055 0.0003 0.0003 0.0002 0 0.0002 0.0002
Nucleus 0.003 0.0014 0.0023 0.0005 0.0008 0.0023 0.009 0.0046 0.001 0.0008 0.0002 0.0012 0.0007 0.0021 0.0006 0.0002 0.0003 0.0003
Peroxisomes 0.0291 0.0164 0.0411 0.0473 0.0522 0.0272 0.0296 0.0095 0.0198 0.0169 0.0505 0.0342 0.0169 0.0114 0.04 0.0237 0.0375 0.0307
Punctate Nuclear 0.0137 0.003 0.0046 0.001 0.0041 0.0121 0.0068 0.0076 0.0028 0.0009 0.0017 0.0082 0.0025 0.0062 0.0014 0.0005 0.0013 0.0024
Vacuole 0.0133 0.0105 0.0172 0.0083 0.0067 0.0079 0.0102 0.0117 0.0133 0.0207 0.0056 0.0081 0.0099 0.0206 0.0137 0.0066 0.005 0.0067
Vacuole Periphery 0.0036 0.0026 0.0031 0.0018 0.004 0.002 0.0019 0.0017 0.0023 0.0048 0.0018 0.0015 0.0013 0.0024 0.0023 0.002 0.0014 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.8713 16.5147 20.053 23.6889 28.3074 18.4638 23.9085 22.54 24.8684 27.9473
Translational Efficiency 0.6601 1.0648 0.856 0.6924 0.6934 1.4218 0.7619 0.9217 0.9303 0.9783

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
97 1483 1966 1981 2412 2405 1004 119 2509 3888 2970 2100

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 730.12 804.87 912.18 847.06 716.38 809.63 812.24 986.98 716.91 807.81 878.40 854.99
Standard Deviation 129.57 100.13 112.20 139.04 81.73 101.58 124.43 125.07 84.13 101.06 125.71 142.02
Intensity Change Log 2 0.140622 0.321185 0.214331 0.176538 0.181181 0.462296 0.158521 0.253545 0.342459

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001119 0.000248 0.000405 0.001536 0.000172 0.000370 0.000621 0.000797 0.000208 0.000323 0.000478 0.001494
Bud Neck 0.053379 0.014968 0.032291 0.020682 0.036035 0.027295 0.011224 0.010433 0.036705 0.022593 0.025170 0.020102
Bud Site 0.083216 0.013258 0.018751 0.045903 0.007200 0.011408 0.018611 0.035543 0.010139 0.012114 0.018704 0.045316
Cell Periphery 0.000517 0.000052 0.000043 0.000162 0.000041 0.000062 0.000079 0.000156 0.000060 0.000059 0.000055 0.000162
Cytoplasm 0.122669* 0.079079 0.128731* 0.401281* 0.128991* 0.071499 0.224237* 0.264969* 0.128747* 0.074390 0.161017* 0.393556*
Cytoplasmic Foci 0.248930* 0.304358* 0.146917 0.088404 0.299101* 0.216707* 0.181419* 0.114234 0.297161* 0.250140* 0.158580 0.089867
Eisosomes 0.000316 0.000054 0.000030 0.000047 0.000091 0.000059 0.000050 0.000096 0.000099 0.000057 0.000037 0.000050
Endoplasmic Reticulum 0.001110 0.001076 0.001826 0.002136 0.000802 0.001123 0.001707 0.002038 0.000814 0.001105 0.001785 0.002131
Endosome 0.262343* 0.410083* 0.245961* 0.047786 0.315219* 0.450054* 0.211334* 0.126579 0.313174* 0.434808* 0.234255* 0.052251
Golgi 0.020765 0.028870 0.003486 0.003885 0.014830 0.015456 0.003162 0.000985 0.015060 0.020572 0.003376 0.003721
Lipid Particles 0.032676 0.016827 0.006357 0.004389 0.026432 0.022034 0.007183 0.005374 0.026673 0.020048 0.006636 0.004444
Mitochondria 0.009856 0.007333 0.002826 0.004868 0.008688 0.010944 0.000682 0.000243 0.008734 0.009567 0.002101 0.004606
Mitotic Spindle 0.001683 0.006330 0.113281* 0.062059 0.003642 0.013283 0.041943 0.009911 0.003567 0.010631 0.089165 0.059104
None 0.000242 0.000363 0.001192 0.003176 0.000470 0.000591 0.001848 0.002034 0.000461 0.000504 0.001414 0.003111
Nuclear Periphery 0.000402 0.000421 0.004179 0.001552 0.000322 0.000605 0.001223 0.000978 0.000325 0.000535 0.003180 0.001519
Nuclear Periphery Foci 0.001148 0.001426 0.010222 0.003290 0.000739 0.001288 0.014537 0.006073 0.000755 0.001340 0.011681 0.003448
Nucleolus 0.005372 0.000704 0.009669 0.001469 0.001021 0.002176 0.005858 0.001034 0.001190 0.001614 0.008381 0.001445
Nucleus 0.037543 0.005468 0.054938 0.084142 0.017855 0.010398 0.048488 0.053947 0.018616 0.008518 0.052757 0.082431
Peroxisomes 0.044983 0.022979 0.027136 0.015111 0.024699 0.021451 0.035644 0.037163 0.025483 0.022034 0.030012 0.016361
Vacuole 0.065259 0.079842 0.189184 0.204151* 0.110508 0.116730 0.188793 0.326854* 0.108758 0.102660 0.189052 0.211104*
Vacuole Periphery 0.006469 0.006258 0.002577 0.003971 0.003142 0.006468 0.001356 0.000557 0.003271 0.006388 0.002164 0.003777

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.58 1.81 0.38 -5.55 -3.29 -1.30 -6.64 -4.21 -1.57 0.78 -1.19 -6.29 -6.01 -4.19 -2.79
Bud Neck 3.22 1.12 2.71 -3.66 8.63 3.71 10.17 9.63 7.16 2.44 6.86 2.56 7.77 1.66 6.43
Bud Site 3.67 3.42 2.90 -6.69 -4.57 -4.39 -9.52 -2.42 -1.65 -0.28 -1.80 -6.83 -8.43 -7.88 -4.56
Cell Periphery 3.17 3.20 2.80 -4.68 -5.11 -3.14 -8.84 -4.80 -3.44 -2.53 0.10 -0.13 -3.99 -4.37 -4.48
Cytoplasm 2.07 -0.96 -11.56 -36.05 -27.05 10.76 -9.78 -5.19 -7.69 -1.06 11.03 -6.57 -29.76 -41.11 -23.99
Cytoplasmic Foci -2.57 5.68 8.11 38.06 12.29 13.95 21.19 19.95 12.46 6.28 8.73 31.87 44.93 42.35 16.16
Eisosomes 4.55 4.87 4.64 1.71 -6.33 5.19 6.70 -1.06 -4.15 -4.50 7.63 11.49 9.41 1.30 -4.22
Endoplasmic Reticulum 0.44 -4.30 -2.48 -4.26 2.42 -1.65 -7.82 -6.05 -5.07 -2.52 -1.86 -11.07 -6.63 -5.03 1.80
Endosome -4.96 2.60 7.94 48.17* 28.52* -15.43 18.15 16.88 25.67 5.74 -15.98 19.73 46.15* 75.78* 31.62*
Golgi -1.46 3.34 3.48 17.95 1.91 -0.62 16.11 19.81 19.26 4.86 -5.40 17.35 16.85 23.09 2.03
Lipid Particles 1.60 2.64 2.89 8.61 5.49 2.10 12.24 12.23 10.49 1.82 3.62 13.03 14.57 14.94 6.25
Mitochondria 1.19 3.62 3.72 4.94 0.74 -2.36 11.47 12.20 15.64 5.77 -1.01 9.64 7.89 10.41 -0.60
Mitotic Spindle -4.20 -22.85* -13.75 -11.88 11.96 -6.80 -10.32 -2.32 -1.28 2.39 -6.70 -24.28 -13.27 -10.62 10.48
None -1.48 -6.51 -6.12 -5.70 -3.21 -1.57 -7.06 -1.90 -1.69 -0.57 -1.08 -7.50 -5.78 -5.49 -2.98
Nuclear Periphery 0.08 -16.55 -13.65 -10.52 10.65 -3.24 -8.76 -7.59 -5.75 0.04 -2.38 -18.95 -20.17 -14.76 9.04
Nuclear Periphery Foci 0.58 -5.85 -0.61 -4.52 8.98 -2.32 -10.30 -6.44 -5.62 4.21 -2.73 -15.38 -8.13 -5.52 11.66
Nucleolus 1.07 -2.19 0.65 -3.50 11.61 -4.74 -7.29 -2.02 0.59 5.71 -2.36 -15.29 -3.57 -1.91 12.27
Nucleus 2.81 -2.66 -5.32 -30.82 -7.18 5.41 -10.09 -5.72 -6.49 -1.46 8.07 -17.47 -26.10 -30.53 -8.95
Peroxisomes 2.55 2.77 4.04 7.16 6.92 1.87 -1.39 1.06 0.57 1.52 2.20 1.19 9.36 8.45 8.76
Vacuole -0.73 -11.22 -16.50 -32.63 -9.95 -1.02 -16.07 -12.22 -12.00 -6.64 1.76 -23.01 -30.17 -32.53 -10.80
Vacuole Periphery 0.08 1.84 1.99 7.59 1.06 -7.85 8.71 13.30 16.14 4.73 -8.60 6.67 4.06 10.42 0.40
Standard name
Human Ortholog
Description Subunit of the BLOC-1 complex involved in endosomal maturation; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome
Localization
Cell Percentages endosome (27%), cytoplasm (13%), mixed (27%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Kxd1

Kxd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kxd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available