Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the BLOC-1 complex involved in endosomal maturation; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 3 | 5 | 0 | 2 | 1 | 0 | – | 0 | 1 | 1 | 0 | 3 | 3 | 1 | 0 | 0 | 1 | 0 | 3 | 2 | 0 | 2 | 2 |
Bud | 0 | 1 | 5 | 3 | 2 | 13 | 4 | – | 5 | 10 | 13 | 6 | 2 | 4 | 2 | 1 | 0 | 1 | 0 | 2 | 1 | 0 | 3 | 5 |
Bud Neck | 0 | 0 | 1 | 1 | 0 | 2 | 1 | – | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 1 | 1 | 2 | 3 | – | 4 | 4 | 4 | 7 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 10 | 202 | 156 | 198 | 245 | 314 | 483 | – | 434 | 484 | 417 | 490 | 103 | 149 | 185 | 183 | 199 | 170 | 13 | 97 | 86 | 62 | 58 | 50 |
Endoplasmic Reticulum | 0 | 9 | 1 | 1 | 0 | 0 | 0 | – | 1 | 0 | 2 | 0 | 7 | 4 | 2 | 14 | 11 | 9 | 0 | 0 | 2 | 2 | 1 | 3 |
Endosome | 4 | 109 | 65 | 62 | 67 | 27 | 49 | – | 18 | 22 | 16 | 16 | 73 | 176 | 171 | 31 | 27 | 30 | 43 | 251 | 141 | 170 | 213 | 277 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 0 | 2 |
Mitochondria | 0 | 6 | 0 | 4 | 6 | 74 | 30 | – | 102 | 82 | 132 | 152 | 0 | 1 | 0 | 8 | 3 | 1 | 4 | 14 | 13 | 9 | 4 | 8 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 2 | 0 | – | 2 | 4 | 3 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 1 | 0 | 2 | 0 | 1 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Nucleolus | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 0 |
Peroxisomes | 0 | 0 | 3 | 1 | 3 | 0 | 3 | – | 1 | 0 | 1 | 1 | 0 | 7 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 1 | 22 | 56 | 25 | 30 | 6 | 33 | – | 5 | 14 | 5 | 5 | 13 | 46 | 39 | 15 | 9 | 6 | 6 | 25 | 33 | 17 | 13 | 27 |
Vac/Vac Membrane | 2 | 52 | 22 | 8 | 36 | 13 | 37 | – | 13 | 12 | 12 | 10 | 24 | 33 | 44 | 18 | 15 | 12 | 4 | 37 | 17 | 25 | 28 | 25 |
Unique Cell Count | 16 | 349 | 268 | 279 | 347 | 394 | 573 | 512 | 567 | 517 | 592 | 195 | 347 | 366 | 235 | 246 | 212 | 76 | 443 | 306 | 297 | 336 | 414 | |
Labelled Cell Count | 18 | 406 | 315 | 305 | 392 | 454 | 643 | 588 | 635 | 606 | 687 | 227 | 425 | 451 | 273 | 271 | 235 | 76 | 443 | 306 | 297 | 336 | 414 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 5.3 | 6.2 | 5.8 | 6.4 | 4.6 | 5.6 | – | 4.8 | 5.2 | 4.9 | 4.9 | 5.9 | 6.4 | 6.6 | 6.2 | 6.4 | 6.0 | 5.2 | 5.4 | 5.8 |
Std Deviation (1e-4) | 0.6 | 0.7 | 1.6 | 1.2 | 1.6 | 1.6 | 1.5 | – | 1.3 | 1.2 | 0.9 | 1.0 | 1.1 | 1.5 | 1.4 | 1.3 | 1.1 | 1.3 | 1.4 | 1.2 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.05 | -0.42 | -0.14 | – | -0.37 | -0.25 | -0.35 | -0.34 | -0.06 | 0.05 | 0.08 | 0.01 | 0.05 | -0.05 | -0.24 | -0.19 | -0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.1 | 3.2 | 6.0 | 8.3 | – | 8.2 | 8.7 | 6.7 | 7.7 | -1.2 | -3.8 | -1.9 | 4.7 | 5.6 | 5.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 3.1 | 0 |
Endosome | -0.6 | -1.5 | 0 | -6.2 | – | 0 | -9.0 | 0 | -10.0 | 3.1 | 6.7 | 5.8 | -3.1 | -3.9 | -2.8 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.8 | – | 0 | 6.6 | 0 | 9.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -3.9 | -4.3 | 0 | -6.6 | – | 0 | -9.0 | 0 | -10.6 | -4.2 | -2.5 | -3.6 | -4.7 | -5.9 | -5.9 |
Vacuole | -2.7 | 0.9 | -2.8 | -0.9 | – | -3.6 | -4.2 | -3.8 | -4.7 | 1.5 | 0.6 | 1.6 | -0.2 | -0.9 | -1.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.2496 | 0.0067 | -0.413 | -0.7569 | -0.0602 | -0.2468 | 1.1593 | 1.8654 | 1.4394 | 1.0424 | 1.0618 | 1.4829 | 3.5143 | 4.5449 | 4.0825 | 4.0584 | 4.1329 | 4.4453 |
Actin | 0.016 | 0.0001 | 0.0092 | 0.0036 | 0.0108 | 0.0008 | 0.0163 | 0.003 | 0.0039 | 0.0004 | 0.0002 | 0.0004 | 0.0011 | 0.0001 | 0.0003 | 0.0001 | 0.0012 | 0.0005 |
Bud | 0.0029 | 0.0011 | 0.0026 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.002 | 0.0036 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.0032 | 0.0003 | 0.0009 | 0.0003 | 0.0005 | 0.0007 | 0.0049 | 0.0003 | 0.0006 | 0.0002 | 0.0002 | 0.0007 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0004 | 0.0005 |
Bud Periphery | 0.0016 | 0.0002 | 0.001 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0.0011 | 0.0041 | 0.0002 | 0.0002 | 0.0001 | 0.0009 | 0.0001 | 0.0009 | 0 | 0.0001 | 0 |
Bud Site | 0.0017 | 0.0113 | 0.0046 | 0.0004 | 0.0008 | 0.0002 | 0.0011 | 0.0037 | 0.0033 | 0.0003 | 0.0001 | 0.0003 | 0.0016 | 0.0009 | 0.001 | 0.0001 | 0.0003 | 0.0002 |
Cell Periphery | 0.0002 | 0.0003 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasm | 0.049 | 0.0491 | 0.076 | 0.0173 | 0.0449 | 0.0786 | 0.0777 | 0.1062 | 0.1041 | 0.0332 | 0.0598 | 0.1042 | 0.1015 | 0.1479 | 0.0971 | 0.0794 | 0.0569 | 0.064 |
Cytoplasmic Foci | 0.274 | 0.3775 | 0.3171 | 0.2828 | 0.2737 | 0.3496 | 0.2818 | 0.3172 | 0.2916 | 0.2792 | 0.2816 | 0.3789 | 0.2892 | 0.2887 | 0.2894 | 0.3255 | 0.3615 | 0.3518 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.004 | 0.0011 | 0.0032 | 0.0007 | 0.0022 | 0.0022 | 0.0045 | 0.0023 | 0.0035 | 0.0011 | 0.0013 | 0.0018 | 0.0033 | 0.0019 | 0.0013 | 0.0005 | 0.001 | 0.0015 |
Endosome | 0.4058 | 0.3718 | 0.3544 | 0.4796 | 0.3391 | 0.3702 | 0.3923 | 0.3747 | 0.4115 | 0.5135 | 0.4964 | 0.3362 | 0.4538 | 0.4202 | 0.4031 | 0.528 | 0.4414 | 0.424 |
Golgi | 0.0234 | 0.0118 | 0.0178 | 0.0292 | 0.0617 | 0.0122 | 0.0171 | 0.0152 | 0.0216 | 0.0369 | 0.0198 | 0.0169 | 0.0137 | 0.0145 | 0.019 | 0.0218 | 0.0294 | 0.016 |
Lipid Particles | 0.0554 | 0.0518 | 0.0471 | 0.0403 | 0.0809 | 0.0569 | 0.0421 | 0.0385 | 0.0362 | 0.0311 | 0.0201 | 0.0315 | 0.0388 | 0.0202 | 0.0236 | 0.006 | 0.0287 | 0.0408 |
Mitochondria | 0.0171 | 0.0009 | 0.0055 | 0.0193 | 0.0513 | 0.0012 | 0.0022 | 0.0014 | 0.0022 | 0.0129 | 0.0018 | 0.004 | 0.0011 | 0.0009 | 0.0028 | 0.0018 | 0.0065 | 0.0013 |
None | 0.0723 | 0.0873 | 0.0881 | 0.0665 | 0.0617 | 0.0704 | 0.0742 | 0.0756 | 0.0724 | 0.0454 | 0.0576 | 0.0648 | 0.0613 | 0.0596 | 0.1019 | 0.0033 | 0.0264 | 0.0568 |
Nuclear Periphery | 0.0094 | 0.0008 | 0.0031 | 0.0005 | 0.0029 | 0.0048 | 0.0048 | 0.002 | 0.0017 | 0.001 | 0.0007 | 0.0013 | 0.0013 | 0.0011 | 0.0008 | 0.0002 | 0.0004 | 0.0005 |
Nucleolus | 0.0011 | 0.0007 | 0.001 | 0.0002 | 0.0003 | 0.0007 | 0.0228 | 0.0214 | 0.0003 | 0.0002 | 0.0001 | 0.0055 | 0.0003 | 0.0003 | 0.0002 | 0 | 0.0002 | 0.0002 |
Nucleus | 0.003 | 0.0014 | 0.0023 | 0.0005 | 0.0008 | 0.0023 | 0.009 | 0.0046 | 0.001 | 0.0008 | 0.0002 | 0.0012 | 0.0007 | 0.0021 | 0.0006 | 0.0002 | 0.0003 | 0.0003 |
Peroxisomes | 0.0291 | 0.0164 | 0.0411 | 0.0473 | 0.0522 | 0.0272 | 0.0296 | 0.0095 | 0.0198 | 0.0169 | 0.0505 | 0.0342 | 0.0169 | 0.0114 | 0.04 | 0.0237 | 0.0375 | 0.0307 |
Punctate Nuclear | 0.0137 | 0.003 | 0.0046 | 0.001 | 0.0041 | 0.0121 | 0.0068 | 0.0076 | 0.0028 | 0.0009 | 0.0017 | 0.0082 | 0.0025 | 0.0062 | 0.0014 | 0.0005 | 0.0013 | 0.0024 |
Vacuole | 0.0133 | 0.0105 | 0.0172 | 0.0083 | 0.0067 | 0.0079 | 0.0102 | 0.0117 | 0.0133 | 0.0207 | 0.0056 | 0.0081 | 0.0099 | 0.0206 | 0.0137 | 0.0066 | 0.005 | 0.0067 |
Vacuole Periphery | 0.0036 | 0.0026 | 0.0031 | 0.0018 | 0.004 | 0.002 | 0.0019 | 0.0017 | 0.0023 | 0.0048 | 0.0018 | 0.0015 | 0.0013 | 0.0024 | 0.0023 | 0.002 | 0.0014 | 0.0016 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.8713 | 16.5147 | 20.053 | 23.6889 | 28.3074 | 18.4638 | 23.9085 | 22.54 | 24.8684 | 27.9473 |
Translational Efficiency | 0.6601 | 1.0648 | 0.856 | 0.6924 | 0.6934 | 1.4218 | 0.7619 | 0.9217 | 0.9303 | 0.9783 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
97 | 1483 | 1966 | 1981 | 2412 | 2405 | 1004 | 119 | 2509 | 3888 | 2970 | 2100 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 730.12 | 804.87 | 912.18 | 847.06 | 716.38 | 809.63 | 812.24 | 986.98 | 716.91 | 807.81 | 878.40 | 854.99 |
Standard Deviation | 129.57 | 100.13 | 112.20 | 139.04 | 81.73 | 101.58 | 124.43 | 125.07 | 84.13 | 101.06 | 125.71 | 142.02 |
Intensity Change Log 2 | — | 0.140622 | 0.321185 | 0.214331 | — | 0.176538 | 0.181181 | 0.462296 | — | 0.158521 | 0.253545 | 0.342459 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001119 | 0.000248 | 0.000405 | 0.001536 | 0.000172 | 0.000370 | 0.000621 | 0.000797 | 0.000208 | 0.000323 | 0.000478 | 0.001494 |
Bud Neck | 0.053379 | 0.014968 | 0.032291 | 0.020682 | 0.036035 | 0.027295 | 0.011224 | 0.010433 | 0.036705 | 0.022593 | 0.025170 | 0.020102 |
Bud Site | 0.083216 | 0.013258 | 0.018751 | 0.045903 | 0.007200 | 0.011408 | 0.018611 | 0.035543 | 0.010139 | 0.012114 | 0.018704 | 0.045316 |
Cell Periphery | 0.000517 | 0.000052 | 0.000043 | 0.000162 | 0.000041 | 0.000062 | 0.000079 | 0.000156 | 0.000060 | 0.000059 | 0.000055 | 0.000162 |
Cytoplasm | 0.122669* | 0.079079 | 0.128731* | 0.401281* | 0.128991* | 0.071499 | 0.224237* | 0.264969* | 0.128747* | 0.074390 | 0.161017* | 0.393556* |
Cytoplasmic Foci | 0.248930* | 0.304358* | 0.146917 | 0.088404 | 0.299101* | 0.216707* | 0.181419* | 0.114234 | 0.297161* | 0.250140* | 0.158580 | 0.089867 |
Eisosomes | 0.000316 | 0.000054 | 0.000030 | 0.000047 | 0.000091 | 0.000059 | 0.000050 | 0.000096 | 0.000099 | 0.000057 | 0.000037 | 0.000050 |
Endoplasmic Reticulum | 0.001110 | 0.001076 | 0.001826 | 0.002136 | 0.000802 | 0.001123 | 0.001707 | 0.002038 | 0.000814 | 0.001105 | 0.001785 | 0.002131 |
Endosome | 0.262343* | 0.410083* | 0.245961* | 0.047786 | 0.315219* | 0.450054* | 0.211334* | 0.126579 | 0.313174* | 0.434808* | 0.234255* | 0.052251 |
Golgi | 0.020765 | 0.028870 | 0.003486 | 0.003885 | 0.014830 | 0.015456 | 0.003162 | 0.000985 | 0.015060 | 0.020572 | 0.003376 | 0.003721 |
Lipid Particles | 0.032676 | 0.016827 | 0.006357 | 0.004389 | 0.026432 | 0.022034 | 0.007183 | 0.005374 | 0.026673 | 0.020048 | 0.006636 | 0.004444 |
Mitochondria | 0.009856 | 0.007333 | 0.002826 | 0.004868 | 0.008688 | 0.010944 | 0.000682 | 0.000243 | 0.008734 | 0.009567 | 0.002101 | 0.004606 |
Mitotic Spindle | 0.001683 | 0.006330 | 0.113281* | 0.062059 | 0.003642 | 0.013283 | 0.041943 | 0.009911 | 0.003567 | 0.010631 | 0.089165 | 0.059104 |
None | 0.000242 | 0.000363 | 0.001192 | 0.003176 | 0.000470 | 0.000591 | 0.001848 | 0.002034 | 0.000461 | 0.000504 | 0.001414 | 0.003111 |
Nuclear Periphery | 0.000402 | 0.000421 | 0.004179 | 0.001552 | 0.000322 | 0.000605 | 0.001223 | 0.000978 | 0.000325 | 0.000535 | 0.003180 | 0.001519 |
Nuclear Periphery Foci | 0.001148 | 0.001426 | 0.010222 | 0.003290 | 0.000739 | 0.001288 | 0.014537 | 0.006073 | 0.000755 | 0.001340 | 0.011681 | 0.003448 |
Nucleolus | 0.005372 | 0.000704 | 0.009669 | 0.001469 | 0.001021 | 0.002176 | 0.005858 | 0.001034 | 0.001190 | 0.001614 | 0.008381 | 0.001445 |
Nucleus | 0.037543 | 0.005468 | 0.054938 | 0.084142 | 0.017855 | 0.010398 | 0.048488 | 0.053947 | 0.018616 | 0.008518 | 0.052757 | 0.082431 |
Peroxisomes | 0.044983 | 0.022979 | 0.027136 | 0.015111 | 0.024699 | 0.021451 | 0.035644 | 0.037163 | 0.025483 | 0.022034 | 0.030012 | 0.016361 |
Vacuole | 0.065259 | 0.079842 | 0.189184 | 0.204151* | 0.110508 | 0.116730 | 0.188793 | 0.326854* | 0.108758 | 0.102660 | 0.189052 | 0.211104* |
Vacuole Periphery | 0.006469 | 0.006258 | 0.002577 | 0.003971 | 0.003142 | 0.006468 | 0.001356 | 0.000557 | 0.003271 | 0.006388 | 0.002164 | 0.003777 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.58 | 1.81 | 0.38 | -5.55 | -3.29 | -1.30 | -6.64 | -4.21 | -1.57 | 0.78 | -1.19 | -6.29 | -6.01 | -4.19 | -2.79 |
Bud Neck | 3.22 | 1.12 | 2.71 | -3.66 | 8.63 | 3.71 | 10.17 | 9.63 | 7.16 | 2.44 | 6.86 | 2.56 | 7.77 | 1.66 | 6.43 |
Bud Site | 3.67 | 3.42 | 2.90 | -6.69 | -4.57 | -4.39 | -9.52 | -2.42 | -1.65 | -0.28 | -1.80 | -6.83 | -8.43 | -7.88 | -4.56 |
Cell Periphery | 3.17 | 3.20 | 2.80 | -4.68 | -5.11 | -3.14 | -8.84 | -4.80 | -3.44 | -2.53 | 0.10 | -0.13 | -3.99 | -4.37 | -4.48 |
Cytoplasm | 2.07 | -0.96 | -11.56 | -36.05 | -27.05 | 10.76 | -9.78 | -5.19 | -7.69 | -1.06 | 11.03 | -6.57 | -29.76 | -41.11 | -23.99 |
Cytoplasmic Foci | -2.57 | 5.68 | 8.11 | 38.06 | 12.29 | 13.95 | 21.19 | 19.95 | 12.46 | 6.28 | 8.73 | 31.87 | 44.93 | 42.35 | 16.16 |
Eisosomes | 4.55 | 4.87 | 4.64 | 1.71 | -6.33 | 5.19 | 6.70 | -1.06 | -4.15 | -4.50 | 7.63 | 11.49 | 9.41 | 1.30 | -4.22 |
Endoplasmic Reticulum | 0.44 | -4.30 | -2.48 | -4.26 | 2.42 | -1.65 | -7.82 | -6.05 | -5.07 | -2.52 | -1.86 | -11.07 | -6.63 | -5.03 | 1.80 |
Endosome | -4.96 | 2.60 | 7.94 | 48.17* | 28.52* | -15.43 | 18.15 | 16.88 | 25.67 | 5.74 | -15.98 | 19.73 | 46.15* | 75.78* | 31.62* |
Golgi | -1.46 | 3.34 | 3.48 | 17.95 | 1.91 | -0.62 | 16.11 | 19.81 | 19.26 | 4.86 | -5.40 | 17.35 | 16.85 | 23.09 | 2.03 |
Lipid Particles | 1.60 | 2.64 | 2.89 | 8.61 | 5.49 | 2.10 | 12.24 | 12.23 | 10.49 | 1.82 | 3.62 | 13.03 | 14.57 | 14.94 | 6.25 |
Mitochondria | 1.19 | 3.62 | 3.72 | 4.94 | 0.74 | -2.36 | 11.47 | 12.20 | 15.64 | 5.77 | -1.01 | 9.64 | 7.89 | 10.41 | -0.60 |
Mitotic Spindle | -4.20 | -22.85* | -13.75 | -11.88 | 11.96 | -6.80 | -10.32 | -2.32 | -1.28 | 2.39 | -6.70 | -24.28 | -13.27 | -10.62 | 10.48 |
None | -1.48 | -6.51 | -6.12 | -5.70 | -3.21 | -1.57 | -7.06 | -1.90 | -1.69 | -0.57 | -1.08 | -7.50 | -5.78 | -5.49 | -2.98 |
Nuclear Periphery | 0.08 | -16.55 | -13.65 | -10.52 | 10.65 | -3.24 | -8.76 | -7.59 | -5.75 | 0.04 | -2.38 | -18.95 | -20.17 | -14.76 | 9.04 |
Nuclear Periphery Foci | 0.58 | -5.85 | -0.61 | -4.52 | 8.98 | -2.32 | -10.30 | -6.44 | -5.62 | 4.21 | -2.73 | -15.38 | -8.13 | -5.52 | 11.66 |
Nucleolus | 1.07 | -2.19 | 0.65 | -3.50 | 11.61 | -4.74 | -7.29 | -2.02 | 0.59 | 5.71 | -2.36 | -15.29 | -3.57 | -1.91 | 12.27 |
Nucleus | 2.81 | -2.66 | -5.32 | -30.82 | -7.18 | 5.41 | -10.09 | -5.72 | -6.49 | -1.46 | 8.07 | -17.47 | -26.10 | -30.53 | -8.95 |
Peroxisomes | 2.55 | 2.77 | 4.04 | 7.16 | 6.92 | 1.87 | -1.39 | 1.06 | 0.57 | 1.52 | 2.20 | 1.19 | 9.36 | 8.45 | 8.76 |
Vacuole | -0.73 | -11.22 | -16.50 | -32.63 | -9.95 | -1.02 | -16.07 | -12.22 | -12.00 | -6.64 | 1.76 | -23.01 | -30.17 | -32.53 | -10.80 |
Vacuole Periphery | 0.08 | 1.84 | 1.99 | 7.59 | 1.06 | -7.85 | 8.71 | 13.30 | 16.14 | 4.73 | -8.60 | 6.67 | 4.06 | 10.42 | 0.40 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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