ORF
Human Ortholog
Description Putative protein of unknown function; MINDY family deubiquitinase that does not appear to contain catalytic activity; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; not an essential gene; YGL082W has a paralog, YPL191C, that arose from the whole genome duplication; ortholog of human MINDY1/FAM63A
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Bud – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Bud Site – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Cytoplasm – – 0.98 0.95 0.91 0.79 0.76 0.68 0.68 0.7 0.67 0.65 0.98 0.96 0.96 0.93 0.92 0.96 – – 0.94 0.96 0.95 0.95
Endoplasmic Reticulum – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 – – 0 0 0 0
Endosome – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Golgi – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Mitochondria – – 0 0 0 0.06 0.12 0.09 0.12 0.2 0.21 0.14 0 0 0 0 0 0 – – 0 0 0 0
Nucleus – – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 – – 0 0 0 0
Nuclear Periphery – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Nucleolus – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
SpindlePole – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Vac/Vac Membrane – – 0 0 0.09 0.17 0.18 0.26 0.23 0.13 0.2 0.21 0 0.05 0.05 0.09 0 0 – – 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Bud – – 0 0 0 1 6 1 1 6 2 6 0 0 0 0 0 0 – – 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 1
Bud Site – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 1 1 4 2 6 6 13 6 2 10 1 6 3 1 6 2 – – 0 0 0 0
Cytoplasm – – 275 271 331 267 405 395 258 303 218 240 344 658 634 127 130 104 – – 260 185 435 420
Endoplasmic Reticulum – – 2 0 2 0 3 2 2 12 2 9 1 2 0 9 0 2 – – 3 1 1 2
Endosome – – 0 0 0 0 2 3 0 0 0 1 1 0 1 0 0 0 – – 0 1 1 1
Golgi – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 1 0 1 1
Mitochondria – – 1 5 7 20 62 53 47 85 68 52 0 0 0 2 1 1 – – 0 0 2 1
Nucleus – – 4 5 2 10 10 19 16 21 5 13 2 3 2 1 5 3 – – 1 2 6 5
Nuclear Periphery – – 1 0 0 0 2 0 0 0 4 2 0 0 0 0 1 0 – – 0 0 0 1
Nucleolus – – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 – – 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
SpindlePole – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 1 0
Vac/Vac Membrane – – 2 12 31 57 98 148 86 56 64 77 7 37 35 12 6 3 – – 5 0 5 1
Unique Cell Count 282 284 362 337 536 580 378 434 324 368 352 682 657 137 141 108 276 194 458 442
Labelled Cell Count 286 294 377 357 595 627 423 489 365 410 356 707 675 152 149 115 276 194 458 442


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 8.8 8.2 8.5 8.0 8.1 8.2 8.1 7.5 7.6 8.3 10.6 11.3 11.1 13.9 13.3 12.0 10.1 10.9 11.8
Std Deviation (1e-4) – – 1.3 1.1 1.2 1.2 1.3 1.6 1.6 1.7 1.7 2.0 1.3 1.5 1.4 3.2 2.8 2.4 1.4 1.5 1.7
Intensity Change (Log2) – – – -0.11 -0.06 -0.13 -0.12 -0.1 -0.12 -0.24 -0.22 -0.09 0.26 0.36 0.34 0.65 0.59 0.45 0.19 0.31 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 2.7 0 0 0 0 0 0 0 0 0
Cytoplasm -1.3 -3.2 -6.9 -8.0 -9.7 -9.4 -9.2 -9.5 -10.1 0.2 -0.8 -0.8 -2.3 -2.6 -0.7
Endoplasmic Reticulum 0 0 0 0 0 0 1.9 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.8 5.7 5.0 5.9 7.7 8.0 6.4 0 0 0 0 0 0
Nucleus 0 0 1.3 0 1.6 2.1 2.4 0 1.7 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.7 4.5 6.8 7.3 9.0 8.2 5.8 7.5 7.8 0 3.4 3.3 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.6653 7.4538 6.455 5.8475 6.0537 6.6137 6.8902 8.8552 8.2335 6.7401 6.5401 8.1069 6.1008 8.586 7.1662 7.1273 6.3766 7.3073
Actin 0.0068 0.0004 0.0108 0.0025 0.0023 0.0022 0.0131 0.0005 0.0055 0.0055 0.0193 0.0072 0.0197 0.005 0.0168 0.0003 0.0002 0.0087
Bud 0.0002 0.0001 0.0007 0.0003 0.0006 0.0002 0.0016 0.0001 0.0004 0.0004 0.0012 0.0001 0.0005 0.0001 0.0002 0.0001 0.0002 0.0009
Bud Neck 0.0008 0.0003 0.001 0.0029 0.0014 0.0024 0.0028 0.0002 0.0023 0.0007 0.0012 0.0011 0.0009 0.0002 0.0006 0.0008 0.0007 0.002
Bud Periphery 0.0001 0 0.0003 0.0003 0.0003 0 0.0013 0 0.0004 0.0007 0.0029 0.0001 0.0006 0.0001 0.0003 0 0.0002 0.0006
Bud Site 0.0008 0.0002 0.0006 0.0003 0.0007 0.0001 0.0067 0.001 0.0088 0.0008 0.0017 0.0001 0.0009 0.0001 0.0011 0.0001 0.0005 0.0003
Cell Periphery 0.0001 0 0 0.0001 0 0 0.0003 0 0.0003 0.0001 0.0002 0 0.0001 0 0.0001 0 0.0001 0
Cytoplasm 0.8939 0.9462 0.9047 0.8861 0.8574 0.916 0.8597 0.9738 0.9037 0.7645 0.7049 0.9318 0.8517 0.9602 0.9248 0.9708 0.916 0.9079
Cytoplasmic Foci 0.0106 0.0013 0.0067 0.015 0.0132 0.0039 0.0166 0.0045 0.0131 0.0214 0.0417 0.0043 0.0254 0.0012 0.0035 0.0013 0.0022 0.0074
Eisosomes 0.0001 0 0.0001 0 0 0 0.0001 0 0 0.0001 0.0002 0 0.0004 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0046 0.0017 0.0026 0.002 0.002 0.0032 0.0033 0.0007 0.0026 0.0019 0.0046 0.0014 0.0058 0.0009 0.0015 0.0014 0.0015 0.0029
Endosome 0.0157 0.0004 0.0065 0.0232 0.0274 0.0041 0.0134 0.0015 0.0156 0.0559 0.0849 0.0049 0.0272 0.0025 0.0062 0.0008 0.0067 0.0127
Golgi 0.0032 0 0.0035 0.0048 0.0052 0.0012 0.005 0.0003 0.0076 0.025 0.0296 0.0027 0.0055 0.0017 0.0033 0 0.0003 0.0036
Lipid Particles 0.0025 0 0.0013 0.0037 0.0093 0.0006 0.0076 0.0007 0.0039 0.0281 0.018 0.0065 0.0064 0.0008 0.0017 0 0.0019 0.0005
Mitochondria 0.007 0.0001 0.0063 0.0033 0.0031 0.0003 0.0076 0.0002 0.0024 0.046 0.0144 0.0006 0.0054 0.0004 0.003 0.0001 0.0015 0.0024
None 0.017 0.0019 0.0077 0.0026 0.0025 0.0029 0.0045 0.0028 0.0017 0.0019 0.0032 0.003 0.0082 0.0011 0.0029 0.0018 0.0018 0.0022
Nuclear Periphery 0.0055 0.0021 0.0028 0.0033 0.0076 0.0034 0.0112 0.0009 0.0017 0.0034 0.0086 0.002 0.0102 0.0016 0.0019 0.0019 0.0056 0.0037
Nucleolus 0.0003 0 0.0001 0.0003 0.0004 0.0001 0.0005 0 0.0002 0.0002 0.0004 0.0002 0.0003 0 0.0002 0 0.0083 0.0001
Nucleus 0.0254 0.0444 0.0385 0.038 0.0479 0.0566 0.0221 0.0114 0.0204 0.0291 0.0376 0.0283 0.0189 0.0233 0.0253 0.0193 0.043 0.0404
Peroxisomes 0.0009 0.0001 0.0035 0.0054 0.005 0.0002 0.0127 0.0005 0.0071 0.0073 0.0081 0.0017 0.0043 0.0002 0.0045 0 0.0002 0.0011
Punctate Nuclear 0.0029 0.0004 0.0016 0.0016 0.0111 0.0016 0.0027 0.0006 0.0007 0.0016 0.0039 0.0033 0.005 0.0004 0.0009 0.0007 0.0029 0.0012
Vacuole 0.0013 0.0003 0.0006 0.0027 0.0016 0.0006 0.0058 0.0002 0.0011 0.003 0.0087 0.0004 0.0016 0.0002 0.0006 0.0004 0.005 0.0009
Vacuole Periphery 0.0004 0 0.0002 0.0016 0.0012 0.0001 0.0016 0 0.0003 0.0022 0.0049 0.0001 0.0009 0.0001 0.0002 0.0001 0.0014 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.1823 18.9357 19.7993 23.5718 25.5912 11.5476 30.2417 29.0748 24.0683 27.642
Translational Efficiency 1.2118 1.6182 1.1998 0.9612 1.072 2.7004 1.0776 1.2125 1.3239 0.9399

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1193 345 1970 488 1609 1870 433 889 2802 2215 2403 1377

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 920.71 1161.88 1296.86 1224.15 964.11 1005.59 1367.65 1292.86 945.63 1029.93 1309.62 1268.51
Standard Deviation 120.08 187.64 167.53 188.70 130.03 137.46 189.27 190.66 127.71 156.99 173.79 192.79
Intensity Change Log 2 — 0.335642 0.494204 0.410962 — 0.060773 0.504429 0.423296 — 0.201585 0.499443 0.417284

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000277 0.000692 0.000414 0.004579 0.000213 0.000180 0.000674 0.033104 0.000240 0.000259 0.000461 0.022995
Bud Neck 0.001385 0.001616 0.002967 0.010716 0.001507 0.002464 0.003556 0.008278 0.001455 0.002332 0.003073 0.009142
Bud Site 0.004953 0.012098 0.004938 0.065514 0.005596 0.007583 0.005794 0.079972 0.005322 0.008286 0.005092 0.074849
Cell Periphery 0.000141 0.000167 0.000127 0.000480 0.000126 0.000083 0.000253 0.000381 0.000132 0.000096 0.000150 0.000416
Cytoplasm 0.747792* 0.715256* 0.494580* 0.538042* 0.762560* 0.771766* 0.451080* 0.477728* 0.756272* 0.762964* 0.486741* 0.499103*
Cytoplasmic Foci 0.078347 0.078416 0.004633 0.024759 0.052186 0.038293 0.009625 0.026879 0.063324 0.044542 0.005532 0.026128
Eisosomes 0.000031 0.000029 0.000008 0.000097 0.000018 0.000017 0.000045 0.000078 0.000024 0.000019 0.000015 0.000085
Endoplasmic Reticulum 0.001472 0.001457 0.001066 0.002266 0.001062 0.000697 0.003876 0.001327 0.001237 0.000815 0.001572 0.001660
Endosome 0.003516 0.005493 0.000600 0.006520 0.001942 0.003122 0.003182 0.002678 0.002612 0.003491 0.001065 0.004040
Golgi 0.001145 0.003962 0.000185 0.017620 0.000876 0.001105 0.000159 0.002197 0.000991 0.001550 0.000180 0.007663
Lipid Particles 0.001225 0.001579 0.000498 0.002083 0.000697 0.000608 0.001782 0.002237 0.000922 0.000759 0.000729 0.002182
Mitochondria 0.001413 0.000532 0.000464 0.000945 0.001478 0.000781 0.000425 0.000538 0.001450 0.000742 0.000457 0.000682
Mitotic Spindle 0.002905 0.008292 0.004422 0.020803 0.003040 0.003136 0.003227 0.075529 0.002982 0.003939 0.004207 0.056134
None 0.006265 0.002815 0.006157 0.006162 0.005982 0.004944 0.011354 0.008110 0.006102 0.004612 0.007094 0.007420
Nuclear Periphery 0.000156 0.000237 0.000647 0.000521 0.000148 0.000156 0.001519 0.000437 0.000152 0.000169 0.000804 0.000467
Nuclear Periphery Foci 0.004341 0.005099 0.001040 0.008308 0.002404 0.001963 0.004016 0.002202 0.003229 0.002452 0.001576 0.004366
Nucleolus 0.000727 0.000165 0.000090 0.000111 0.000179 0.000218 0.000245 0.000169 0.000412 0.000210 0.000118 0.000148
Nucleus 0.099010 0.086456 0.454103* 0.144085* 0.129390 0.107671 0.286924* 0.149806* 0.116455 0.104366 0.423979* 0.147779*
Peroxisomes 0.000575 0.001107 0.000117 0.002541 0.000324 0.001358 0.000149 0.012152 0.000431 0.001319 0.000123 0.008746
Vacuole 0.043897 0.074254 0.022885 0.138399 0.029753 0.052877 0.211877* 0.116096 0.035775 0.056207 0.056940 0.124000
Vacuole Periphery 0.000429 0.000278 0.000058 0.005448 0.000518 0.000980 0.000238 0.000102 0.000480 0.000870 0.000091 0.001996

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.97 -4.47 -3.28 -2.74 -2.92 0.68 -9.57 -4.76 -4.78 -4.30 -0.48 -7.91 -5.42 -5.41 -5.06
Bud Neck -1.24 -6.06 -5.09 -4.78 -1.71 -1.67 -10.18 -6.50 -4.27 -0.12 -1.91 -8.13 -8.28 -5.38 -2.17
Bud Site -2.97 -0.99 -5.57 -3.47 -5.30 -1.27 -2.74 -7.73 -7.07 -6.88 -2.34 -1.40 -9.57 -8.14 -9.05
Cell Periphery -1.06 -2.83 -5.09 -4.48 -4.42 2.40 -7.71 -7.32 -11.35 -0.48 3.13 -5.23 -8.83 -11.16 -6.41
Cytoplasm 4.48 38.89 25.66 16.33 3.40 0.05 38.03 37.86 37.28 -7.12 0.97 55.81 45.53 42.13 2.54
Cytoplasmic Foci 0.32 23.00 16.43 10.52 -7.58 5.20 20.13 12.20 8.37 -6.67 7.80 30.79 19.93 12.46 -10.32
Eisosomes 0.25 9.11 -2.69 -2.72 -3.90 0.68 -14.23 -9.89 -10.06 -1.80 3.20 2.01 -7.21 -7.93 -7.65
Endoplasmic Reticulum -0.07 -0.48 -1.99 -1.70 -1.89 3.19 -11.71 -3.15 -6.73 10.51 4.17 -7.53 -3.30 -7.49 4.26
Endosome -1.28 8.89 3.42 2.82 -2.72 -2.53 -2.37 2.38 4.99 6.61 -1.83 6.84 4.53 5.15 -1.83
Golgi -1.17 2.48 -0.75 0.24 -1.31 -0.41 2.89 1.43 1.64 -0.95 -0.88 3.75 -0.25 0.26 -1.51
Lipid Particles -0.55 2.55 -9.17 -3.83 -11.09 0.81 -12.50 -11.56 -11.88 -2.18 1.55 -4.47 -14.38 -14.01 -12.66
Mitochondria 1.21 1.03 1.36 0.11 3.19 0.98 1.17 1.59 1.42 6.19 1.34 1.56 2.10 1.38 6.95
Mitotic Spindle -1.06 -2.15 -3.62 -2.00 -2.81 0.10 -2.86 -5.53 -5.57 -4.24 -0.47 -3.03 -6.59 -6.26 -5.34
None 2.66 0.36 0.98 -2.81 1.41 1.65 0.42 1.13 -0.55 0.41 2.21 0.53 1.44 -1.03 1.55
Nuclear Periphery -5.58 -26.14 -13.34 -10.81 0.80 -1.68 -21.60 -15.62 -15.33 12.45 -3.40 -32.30 -20.37 -19.42 5.87
Nuclear Periphery Foci -1.19 6.58 -0.10 0.55 -2.43 1.41 -5.19 -0.25 -2.01 5.57 2.55 5.15 0.10 -1.39 -2.61
Nucleolus 1.64 1.75 1.67 0.35 -1.93 -1.53 -2.46 -0.79 0.52 1.56 1.32 1.74 1.51 0.99 -1.47
Nucleus 0.48 -62.40 -9.84 -8.49 32.28 4.43 -16.69 -9.66 -12.78 10.54 2.40 -61.72 -13.95 -15.18 38.33
Peroxisomes -1.70 4.00 -0.82 -0.07 -1.58 -1.80 2.83 -3.75 -2.12 -3.95 -1.80 5.01 -3.73 -1.78 -4.29
Vacuole -6.97 -1.34 -21.61 -15.29 -21.36 -9.12 -21.39 -29.47 -25.59 0.89 -9.83 -15.36 -36.34 -31.18 -25.73
Vacuole Periphery 1.47 3.17 -0.56 -0.84 -1.11 -0.87 1.88 2.49 2.00 6.66 -0.89 3.42 0.57 1.13 -1.02
ORF
Human Ortholog
Description Putative protein of unknown function; MINDY family deubiquitinase that does not appear to contain catalytic activity; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; not an essential gene; YGL082W has a paralog, YPL191C, that arose from the whole genome duplication; ortholog of human MINDY1/FAM63A
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YGL082W

YGL082W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ygl082w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available