Standard name | |
---|---|
Human Ortholog | |
Description | Putative kinase; suppressor of GTPase mutant; similar to bovine rhodopsin kinase; may have a role in intracellular sterol transport |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 2 | 5 | 2 | 0 | 0 | 4 | 3 | 1 | 2 | 0 | 0 | 7 | 2 | 3 | 16 | 13 | 16 | 2 | 10 | 5 | 14 | 23 | 22 |
Bud | 4 | 13 | 12 | 5 | 10 | 16 | 42 | 36 | 32 | 26 | 18 | 20 | 7 | 7 | 10 | 1 | 0 | 6 | 0 | 2 | 1 | 1 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 4 | 10 | 12 | 15 | 20 | 23 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 17 | 16 | 2 | 15 | 26 | 31 | 32 | 26 | 30 | 26 | 15 | 13 | 25 | 31 | 21 | 10 | 14 | 0 | 0 | 3 | 0 | 1 | 0 |
Endoplasmic Reticulum | 2 | 9 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 10 | 4 | 8 | 7 | 3 | 3 | 0 | 0 | 2 | 1 | 3 | 2 |
Endosome | 5 | 25 | 56 | 10 | 56 | 72 | 36 | 30 | 11 | 2 | 4 | 7 | 52 | 150 | 137 | 55 | 56 | 44 | 25 | 113 | 73 | 65 | 112 | 105 |
Golgi | 4 | 4 | 6 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 13 | 22 | 28 | 51 | 64 | 59 | 30 | 49 | 37 | 55 | 107 | 110 |
Mitochondria | 55 | 162 | 53 | 41 | 70 | 141 | 197 | 138 | 216 | 243 | 187 | 132 | 149 | 151 | 133 | 24 | 31 | 22 | 7 | 38 | 47 | 40 | 53 | 44 |
Nucleus | 0 | 1 | 1 | 4 | 1 | 4 | 11 | 6 | 4 | 7 | 5 | 6 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 5 | 8 | 0 | 2 | 0 | 1 | 3 | 10 | 8 | 12 | 7 | 5 | 1 | 4 | 7 | 3 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 3 | 5 | 18 | 8 | 47 | 51 | 69 | 42 | 27 | 7 | 13 | 15 | 28 | 68 | 56 | 3 | 3 | 1 | 15 | 26 | 30 | 48 | 84 | 72 |
SpindlePole | 5 | 20 | 45 | 15 | 52 | 51 | 98 | 92 | 56 | 85 | 41 | 52 | 15 | 44 | 27 | 43 | 33 | 38 | 2 | 22 | 7 | 7 | 10 | 6 |
Vac/Vac Membrane | 3 | 7 | 13 | 2 | 4 | 8 | 4 | 5 | 4 | 6 | 2 | 3 | 8 | 6 | 17 | 10 | 14 | 11 | 1 | 7 | 4 | 5 | 4 | 4 |
Unique Cell Count | 64 | 223 | 174 | 66 | 186 | 263 | 334 | 285 | 287 | 321 | 240 | 178 | 227 | 357 | 362 | 179 | 175 | 162 | 88 | 276 | 219 | 246 | 408 | 375 |
Labelled Cell Count | 92 | 273 | 228 | 93 | 261 | 376 | 506 | 410 | 402 | 442 | 327 | 267 | 306 | 486 | 459 | 234 | 230 | 217 | 88 | 276 | 219 | 246 | 408 | 375 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.0 | 5.2 | 6.3 | 5.5 | 6.4 | 5.4 | 5.2 | 5.6 | 4.6 | 4.4 | 4.2 | 4.5 | 5.8 | 5.9 | 6.2 | 8.8 | 9.5 | 8.3 | 5.4 | 5.6 | 5.9 |
Std Deviation (1e-4) | 0.6 | 0.7 | 1.2 | 1.8 | 1.7 | 1.7 | 1.5 | 1.7 | 1.4 | 1.2 | 1.3 | 1.1 | 0.8 | 1.0 | 1.0 | 1.3 | 1.8 | 1.5 | 0.8 | 1.0 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.19 | 0.02 | -0.23 | -0.29 | -0.17 | -0.46 | -0.54 | -0.59 | -0.49 | -0.12 | -0.09 | -0.03 | 0.48 | 0.59 | 0.39 | -0.22 | -0.18 | -0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.1 | 0 | 0 | 2.4 | 1.9 | 2.6 |
Bud | 0 | -0.6 | -0.3 | 2.0 | 1.9 | 0 | 0 | 0 | 0 | -1.8 | -2.9 | -2.3 | -3.2 | -3.5 | -1.3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | -0.4 | 0.2 | 0 | 0.7 | 0 | 0.1 | 0.5 | -0.3 | -1.3 | -0.9 | -0.2 | 0.8 | -1.2 | -0.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 |
Endosome | -2.6 | -0.4 | -1.1 | -5.9 | -5.8 | 0 | 0 | 0 | 0 | -2.1 | 2.2 | 1.3 | -0.3 | 0 | -1.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 1.3 | 1.9 | 6.4 | 7.7 | 7.6 |
Mitochondria | 4.5 | 1.4 | 4.8 | 6.1 | 3.8 | 0 | 0 | 0 | 0 | 7.0 | 2.6 | 1.4 | -3.9 | -2.8 | -3.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.4 | 3.7 | 2.5 | 2.9 | 1.4 | 0 | 0 | 0 | 0 | 0.6 | 2.6 | 1.6 | -3.4 | -3.4 | -3.9 |
SpindlePole | -0.5 | 0.4 | -1.6 | 0.8 | 1.5 | 0 | 0 | 0 | 0 | -5.4 | -3.9 | -5.9 | -0.4 | -1.6 | -0.5 |
Vacuole | 0 | -2.4 | -2.1 | -3.7 | -3.1 | -3.4 | -3.1 | -3.6 | -2.6 | -1.8 | -3.4 | -1.3 | -0.7 | 0.2 | -0.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.9655 | 3.5479 | 3.1167 | 2.7085 | 2.6308 | 3.0835 | 0.6708 | 1.1057 | 0.9923 | 1.0979 | 0.5124 | 0.9443 | 0.4008 | 1.2841 | 0.9543 | 0.8544 | 1.0257 | 0.9092 |
Actin | 0.032 | 0.0191 | 0.0114 | 0.0018 | 0.0431 | 0.0168 | 0.0333 | 0.0364 | 0.0213 | 0.0031 | 0.0177 | 0.0105 | 0.0327 | 0.0171 | 0.0245 | 0.0042 | 0.063 | 0.0192 |
Bud | 0.0003 | 0.001 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0009 | 0.0008 | 0.0008 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0007 | 0.0002 |
Bud Neck | 0.0015 | 0.0037 | 0.0017 | 0.001 | 0.0023 | 0.0022 | 0.0012 | 0.0008 | 0.0009 | 0.001 | 0.0004 | 0.001 | 0.001 | 0.0005 | 0.0013 | 0.001 | 0.0013 | 0.0009 |
Bud Periphery | 0.0004 | 0.0014 | 0.0003 | 0.0001 | 0.0005 | 0.0005 | 0.0004 | 0.0006 | 0.0009 | 0.0012 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0014 | 0.0003 |
Bud Site | 0.0026 | 0.0241 | 0.0114 | 0.0005 | 0.0009 | 0.0009 | 0.002 | 0.0098 | 0.0065 | 0.003 | 0.0007 | 0.0004 | 0.003 | 0.0009 | 0.001 | 0.0008 | 0.0015 | 0.0004 |
Cell Periphery | 0.0004 | 0.0012 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 |
Cytoplasm | 0.0201 | 0.0125 | 0.0526 | 0.0033 | 0.0072 | 0.0105 | 0.013 | 0.0196 | 0.0086 | 0.0034 | 0.0026 | 0.0041 | 0.0167 | 0.0291 | 0.0049 | 0.0226 | 0.0037 | 0.0024 |
Cytoplasmic Foci | 0.2254 | 0.2608 | 0.1791 | 0.2411 | 0.1741 | 0.1902 | 0.2195 | 0.2812 | 0.2069 | 0.1469 | 0.231 | 0.1992 | 0.2694 | 0.2487 | 0.2239 | 0.2205 | 0.1702 | 0.1879 |
Eisosomes | 0.0016 | 0.0011 | 0.0002 | 0 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.0032 | 0.0019 | 0.003 | 0.0002 | 0.0023 | 0.0004 | 0.0032 | 0.0013 | 0.0008 | 0.0008 | 0.0009 | 0.0003 | 0.0046 | 0.0036 | 0.0006 | 0.0012 | 0.0014 | 0.0011 |
Endosome | 0.1856 | 0.2535 | 0.1843 | 0.2768 | 0.2555 | 0.1707 | 0.327 | 0.2453 | 0.2753 | 0.3878 | 0.3037 | 0.2547 | 0.2712 | 0.3463 | 0.2894 | 0.3406 | 0.3994 | 0.2307 |
Golgi | 0.1152 | 0.1637 | 0.1528 | 0.121 | 0.162 | 0.1607 | 0.2 | 0.188 | 0.2334 | 0.2456 | 0.2494 | 0.3016 | 0.1455 | 0.2021 | 0.254 | 0.2273 | 0.1615 | 0.2874 |
Lipid Particles | 0.1054 | 0.0337 | 0.0456 | 0.0687 | 0.0301 | 0.0933 | 0.0469 | 0.0196 | 0.0327 | 0.012 | 0.0374 | 0.0344 | 0.0708 | 0.0208 | 0.0335 | 0.0541 | 0.0946 | 0.076 |
Mitochondria | 0.0564 | 0.0499 | 0.0516 | 0.0596 | 0.0237 | 0.0464 | 0.0208 | 0.0209 | 0.0338 | 0.0328 | 0.016 | 0.0192 | 0.007 | 0.0067 | 0.0103 | 0.009 | 0.0143 | 0.0383 |
None | 0.0456 | 0.0226 | 0.0206 | 0.0008 | 0.0048 | 0.0102 | 0.0084 | 0.005 | 0.0019 | 0.0002 | 0.0023 | 0.0002 | 0.0249 | 0.0118 | 0.0142 | 0.0011 | 0.0009 | 0.0004 |
Nuclear Periphery | 0.002 | 0.0006 | 0.0077 | 0.0001 | 0.0006 | 0.0005 | 0.0049 | 0.0004 | 0.0006 | 0.0004 | 0.0036 | 0.0001 | 0.0044 | 0.0007 | 0.0002 | 0.0004 | 0.0008 | 0.0006 |
Nucleolus | 0.0016 | 0.0007 | 0.0011 | 0.0002 | 0.0002 | 0.0004 | 0.0003 | 0.0005 | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0001 |
Nucleus | 0.001 | 0.0006 | 0.0787 | 0.0001 | 0.0002 | 0.0075 | 0.0007 | 0.0003 | 0.0005 | 0.0004 | 0.0005 | 0.0001 | 0.0013 | 0.0005 | 0.0001 | 0.0002 | 0.0004 | 0.0001 |
Peroxisomes | 0.1866 | 0.1409 | 0.1906 | 0.2202 | 0.2857 | 0.2795 | 0.1036 | 0.1592 | 0.163 | 0.1503 | 0.1258 | 0.171 | 0.1366 | 0.1051 | 0.1324 | 0.1093 | 0.0483 | 0.1492 |
Punctate Nuclear | 0.0087 | 0.0011 | 0.0024 | 0.0015 | 0.0007 | 0.0056 | 0.0047 | 0.002 | 0.0007 | 0.0003 | 0.0029 | 0.0004 | 0.0043 | 0.0012 | 0.0056 | 0.0011 | 0.0022 | 0.001 |
Vacuole | 0.0029 | 0.0047 | 0.0027 | 0.0016 | 0.0036 | 0.0021 | 0.0067 | 0.0069 | 0.0088 | 0.0063 | 0.0019 | 0.0012 | 0.0041 | 0.003 | 0.0023 | 0.0042 | 0.0302 | 0.0018 |
Vacuole Periphery | 0.0013 | 0.0012 | 0.0016 | 0.0012 | 0.0016 | 0.0009 | 0.0025 | 0.001 | 0.0021 | 0.0032 | 0.002 | 0.0009 | 0.0013 | 0.0013 | 0.001 | 0.0019 | 0.0032 | 0.0019 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.8534 | 11.8447 | 7.2739 | 9.5232 | 7.1086 | 5.9364 | 13.7313 | 8.2225 | 6.9837 | 11.6839 |
Translational Efficiency | 1.0447 | 0.7166 | 0.7162 | 0.436 | 0.7946 | 1.0496 | 0.6746 | 0.7591 | 0.779 | 0.6482 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1862 | 1864 | 1284 | 140 | 1931 | 1929 | 346 | 1105 | 3793 | 3793 | 1630 | 1245 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 728.81 | 840.60 | 1042.34 | 1057.88 | 810.87 | 835.49 | 1047.90 | 996.66 | 770.59 | 838.00 | 1043.52 | 1003.54 |
Standard Deviation | 199.61 | 106.83 | 142.51 | 135.62 | 92.84 | 106.19 | 112.66 | 118.79 | 160.09 | 106.54 | 136.74 | 122.34 |
Intensity Change Log 2 | — | 0.205877 | 0.516211 | 0.537561 | — | 0.043152 | 0.369959 | 0.297631 | — | 0.122469 | 0.441038 | 0.416185 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000894 | 0.001238 | 0.028470 | 0.020085 | 0.001201 | 0.001463 | 0.006411 | 0.004914 | 0.001050 | 0.001352 | 0.023788 | 0.006620 |
Bud Neck | 0.003476 | 0.000831 | 0.001086 | 0.003440 | 0.001565 | 0.001521 | 0.001681 | 0.001609 | 0.002503 | 0.001182 | 0.001212 | 0.001815 |
Bud Site | 0.007374 | 0.004192 | 0.010339 | 0.056466 | 0.004077 | 0.003688 | 0.008318 | 0.015134 | 0.005695 | 0.003936 | 0.009910 | 0.019782 |
Cell Periphery | 0.000097 | 0.000034 | 0.000073 | 0.000083 | 0.000047 | 0.000065 | 0.000045 | 0.000042 | 0.000072 | 0.000050 | 0.000067 | 0.000047 |
Cytoplasm | 0.009681 | 0.005188 | 0.003106 | 0.014424 | 0.005486 | 0.004653 | 0.003300 | 0.009081 | 0.007545 | 0.004916 | 0.003147 | 0.009682 |
Cytoplasmic Foci | 0.233547* | 0.293966* | 0.401773* | 0.328324* | 0.250678* | 0.248838* | 0.365627* | 0.303851* | 0.242269* | 0.271015* | 0.394100* | 0.306603* |
Eisosomes | 0.000074 | 0.000035 | 0.000074 | 0.000055 | 0.000066 | 0.000063 | 0.000039 | 0.000032 | 0.000070 | 0.000050 | 0.000067 | 0.000034 |
Endoplasmic Reticulum | 0.000143 | 0.000224 | 0.002236 | 0.000319 | 0.000160 | 0.000140 | 0.000816 | 0.000229 | 0.000152 | 0.000181 | 0.001934 | 0.000239 |
Endosome | 0.149214 | 0.190388 | 0.153544 | 0.170154 | 0.157617 | 0.176329 | 0.217609* | 0.268075* | 0.153492 | 0.183238 | 0.167143 | 0.257064* |
Golgi | 0.418121* | 0.399624* | 0.206381* | 0.142494* | 0.450156* | 0.443696* | 0.140011* | 0.201188* | 0.434430* | 0.422038* | 0.192292* | 0.194588* |
Lipid Particles | 0.010251 | 0.005955 | 0.018831 | 0.004080 | 0.007712 | 0.009999 | 0.013995 | 0.003058 | 0.008958 | 0.008012 | 0.017804 | 0.003173 |
Mitochondria | 0.070826 | 0.026585 | 0.003247 | 0.002462 | 0.050768 | 0.040724 | 0.001976 | 0.009755 | 0.060615 | 0.033776 | 0.002977 | 0.008935 |
Mitotic Spindle | 0.011754 | 0.026304 | 0.119000* | 0.091314 | 0.014759 | 0.012280 | 0.123602* | 0.103814* | 0.013284 | 0.019172 | 0.119977* | 0.102409* |
None | 0.000586 | 0.000064 | 0.000023 | 0.000020 | 0.000093 | 0.000073 | 0.000020 | 0.000631 | 0.000335 | 0.000069 | 0.000022 | 0.000563 |
Nuclear Periphery | 0.000043 | 0.000039 | 0.000271 | 0.000083 | 0.000039 | 0.000056 | 0.000203 | 0.000185 | 0.000041 | 0.000048 | 0.000257 | 0.000173 |
Nuclear Periphery Foci | 0.000222 | 0.000281 | 0.005165 | 0.001749 | 0.000306 | 0.000242 | 0.004657 | 0.000695 | 0.000265 | 0.000261 | 0.005057 | 0.000814 |
Nucleolus | 0.000735 | 0.000334 | 0.000037 | 0.000072 | 0.000319 | 0.000329 | 0.000127 | 0.000113 | 0.000523 | 0.000331 | 0.000056 | 0.000108 |
Nucleus | 0.001693 | 0.000336 | 0.000322 | 0.000139 | 0.000205 | 0.000336 | 0.000429 | 0.000136 | 0.000936 | 0.000336 | 0.000345 | 0.000136 |
Peroxisomes | 0.074823 | 0.036217 | 0.026986 | 0.133264* | 0.046637 | 0.046906 | 0.090396 | 0.041614 | 0.060474 | 0.041653 | 0.040446 | 0.051920 |
Vacuole | 0.003763 | 0.005205 | 0.016837 | 0.028963 | 0.004787 | 0.004752 | 0.019113 | 0.030946 | 0.004284 | 0.004975 | 0.017320 | 0.030723 |
Vacuole Periphery | 0.002682 | 0.002960 | 0.002201 | 0.002010 | 0.003320 | 0.003843 | 0.001626 | 0.004899 | 0.003007 | 0.003409 | 0.002079 | 0.004574 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.09 | -10.55 | -3.04 | -2.98 | 1.31 | -1.08 | -4.06 | -4.24 | -3.92 | 0.98 | -1.52 | -10.89 | -5.28 | -4.95 | 7.40 |
Bud Neck | 6.61 | 5.95 | -0.20 | -2.64 | -2.36 | 0.11 | -0.32 | -0.75 | -0.80 | -0.34 | 4.59 | 4.94 | 1.26 | -2.80 | -2.85 |
Bud Site | 2.75 | -2.55 | -4.59 | -4.89 | -4.28 | 0.58 | -3.55 | -7.04 | -7.37 | -3.89 | 2.65 | -4.89 | -7.51 | -8.56 | -5.05 |
Cell Periphery | 3.39 | 1.20 | 0.46 | -3.49 | -0.78 | -1.84 | 0.13 | 0.96 | 2.44 | 0.65 | 2.10 | 0.33 | 2.48 | 0.47 | 4.34 |
Cytoplasm | 3.33 | 5.35 | -1.66 | -3.25 | -3.95 | 1.03 | 2.21 | -3.41 | -4.26 | -4.95 | 3.34 | 5.66 | -2.41 | -4.81 | -6.28 |
Cytoplasmic Foci | -12.00 | -24.11 | -4.80 | -1.53 | 4.01 | 0.44 | -9.56 | -7.47 | -7.74 | 5.00 | -8.29 | -25.77 | -10.08 | -5.25 | 11.63 |
Eisosomes | 5.25 | -0.13 | 1.60 | -2.78 | 2.10 | 0.51 | 4.82 | 7.32 | 6.52 | 1.64 | 4.41 | 0.53 | 7.49 | 4.48 | 8.56 |
Endoplasmic Reticulum | -1.42 | -7.24 | -2.49 | -1.48 | 6.23 | 0.60 | -3.16 | -1.38 | -1.78 | 2.79 | -0.90 | -7.66 | -2.16 | -1.33 | 7.20 |
Endosome | -7.40 | -0.73 | -1.30 | 1.67 | -1.05 | -3.48 | -5.49 | -14.73 | -12.01 | -4.05 | -7.68 | -2.98 | -15.67 | -10.95 | -12.96 |
Golgi | 2.38 | 28.27 | 17.16 | 16.17 | 4.15 | 0.80 | 31.91 | 31.63 | 30.97 | -5.81 | 2.23 | 41.56 | 36.07 | 34.70 | 0.01 |
Lipid Particles | 3.09 | -4.68 | 3.45 | 0.80 | 7.74 | -1.65 | -2.28 | 4.37 | 5.36 | 3.92 | 1.00 | -6.09 | 6.13 | 5.12 | 10.18 |
Mitochondria | 11.51 | 19.36 | 19.38 | 12.20 | 0.66 | 2.84 | 17.48 | 13.11 | 11.38 | -4.52 | 10.27 | 25.49 | 20.02 | 12.48 | -3.83 |
Mitotic Spindle | -6.31 | -16.94 | -5.14 | -4.21 | 1.48 | 1.40 | -8.66 | -13.36 | -13.77 | 1.19 | -4.03 | -18.87 | -14.58 | -13.53 | 1.80 |
None | 2.70 | 2.90 | 2.91 | 3.66 | 0.56 | 1.02 | 3.90 | -1.08 | -1.12 | -1.22 | 2.79 | 3.25 | -0.53 | -1.12 | -1.21 |
Nuclear Periphery | 0.25 | -7.32 | -1.41 | -1.53 | 3.21 | -0.65 | -3.54 | -2.06 | -1.77 | 0.17 | -0.40 | -8.47 | -2.15 | -2.03 | 1.30 |
Nuclear Periphery Foci | -0.84 | -5.68 | -1.38 | -1.35 | 1.45 | 1.05 | -2.55 | -1.81 | -2.96 | 2.34 | 0.54 | -6.15 | -2.59 | -2.81 | 5.17 |
Nucleolus | 1.28 | 2.16 | 2.01 | 1.42 | -1.16 | 0.09 | 1.83 | 1.90 | 1.54 | 0.08 | 1.20 | 2.81 | 2.44 | 1.92 | -1.34 |
Nucleus | 1.84 | 1.71 | 2.10 | 0.78 | 0.94 | -0.81 | -0.86 | 0.90 | 1.12 | 1.04 | 1.60 | 1.29 | 2.20 | 1.43 | 1.35 |
Peroxisomes | 9.70 | 12.02 | -3.29 | -5.53 | -6.05 | -0.08 | -4.41 | 1.30 | 1.39 | 4.87 | 7.04 | 5.80 | 2.17 | -2.68 | -2.60 |
Vacuole | -2.58 | -10.49 | -4.70 | -4.48 | -2.54 | 0.04 | -4.84 | -10.73 | -10.72 | -3.71 | -1.77 | -11.26 | -11.83 | -11.53 | -5.80 |
Vacuole Periphery | -0.91 | 1.62 | 1.20 | 1.64 | 0.37 | -1.09 | 4.15 | -2.36 | -1.41 | -4.94 | -1.40 | 3.65 | -2.76 | -1.95 | -4.42 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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