Standard name
Human Ortholog
Description Nucleotide exchange factor for Gsp1p; localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p; human homolog of the RAN GEF, RCC1, can complement a temperature sensitive point mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0.05 0.06 0 0 0.06 0.07 0 0.05 0 0.05 0.06 0.09 0 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.07 0.06 0 0 0.06 0.08 0.07 0.11 0.09 0.1 0.11 0 0 0 0.15 0.22 0.06 0 0 0 0 0 0
Nucleus 0.88 0.8 0.73 0.81 0.85 0.78 0.71 0.7 0.64 0.68 0.61 0.67 0.81 0.75 0.7 0.87 0.76 0.82 0.72 0.83 0.61 0.54 0.52 0.49
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.64 0.65 0.6 0.45 0.31 0.45 0.52 0.49 0.61 0.62 0.67 0.66 0.6 0.56 0.48 0.46 0.42 0.45 0.24 0.11 0.27 0.41 0.4 0.38
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 1 2 3
Bud Neck 0 0 0 0 0 1 1 1 3 2 1 0 0 0 0 0 0 0 1 0 0 0 0 2
Bud Site 1 0 0 0 0 0 4 2 2 5 3 2 1 4 5 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 0 9 8 14 8 12 23 23 15 13 8 10 28 46 7 8 5 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 1 0 0 0 1 0 0
Endosome 0 0 0 0 0 0 0 1 0 0 1 0 0 3 4 1 0 0 0 0 0 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Mitochondria 13 3 8 5 5 13 33 28 37 35 25 24 3 5 4 31 33 9 0 0 2 1 0 2
Nucleus 180 32 94 129 202 181 306 277 220 256 161 142 177 355 342 182 112 119 147 58 80 100 176 203
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 132 26 77 72 74 105 225 195 208 234 177 139 130 265 232 96 62 66 48 7 35 76 136 158
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 4 7 1 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 1 10 19 0 1 1 0 0 0 0 3 6
Vac/Vac Membrane 1 1 0 0 1 0 2 1 2 3 0 0 1 4 5 1 3 0 2 2 2 3 15 33
Unique Cell Count 205 40 128 159 238 231 433 397 342 376 263 211 218 472 487 209 148 146 204 70 132 186 339 418
Labelled Cell Count 330 62 188 214 297 309 583 531 495 550 381 315 323 679 664 320 220 202 204 70 132 186 339 418


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.7 20.0 19.6 21.4 21.9 18.5 16.8 16.5 18.0 16.4 16.7 17.3 25.9 26.5 25.2 39.4 40.2 43.8 24.4 22.8 23.9
Std Deviation (1e-4) 8.6 7.1 9.7 8.2 9.6 8.1 7.7 7.0 9.7 8.9 9.6 9.0 9.3 11.5 13.5 18.0 20.8 22.8 9.6 7.7 9.5
Intensity Change (Log2) – – – 0.13 0.16 -0.08 -0.23 -0.25 -0.12 -0.26 -0.24 -0.18 0.4 0.43 0.36 1.01 1.03 1.16 0.31 0.22 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 -0.4 -1.5 0 -0.5 -0.1 -1.4 -0.8 -1.3 -1.0 -0.5 0.9 -1.5 -0.6 -1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.3 0 -0.2 0.5 0.3 1.5 1.1 1.1 1.6 0 0 0 2.4 3.7 0
Nucleus 1.6 2.7 1.1 -0.6 -0.8 -1.9 -1.1 -2.4 -1.2 1.7 0.4 -0.7 3.2 0.4 1.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.5 -5.4 -2.7 -1.6 -2.2 0.1 0.4 1.4 1.1 -0.1 -0.8 -2.5 -2.5 -3.0 -2.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.3908 22.6727 20.6937 22.9747 17.0677 21.4486 21.0814 20.1817 22.0225 22.4883 18.6424 18.4352 31.6632 27.421 24.2836 24.5127 26.043 24.8273
Actin 0.0222 0 0.019 0.0281 0.0099 0.0002 0.0122 0 0.0031 0 0.0302 0.0002 0.012 0.0019 0.0024 0.0084 0.0216 0.0007
Bud 0.0004 0 0.0003 0.0003 0.0006 0.0001 0.0009 0 0.0001 0 0.0007 0 0.0003 0.0003 0.0002 0.0003 0.0002 0
Bud Neck 0.0006 0 0.0004 0.0001 0.0008 0.0001 0.0003 0 0 0 0.0003 0.0004 0.0002 0 0.0001 0.0001 0.0002 0.0001
Bud Periphery 0.0006 0 0.0004 0.0002 0.0008 0.0002 0.0011 0 0.0002 0 0.0012 0 0.0008 0.0003 0.0002 0.0003 0.0003 0
Bud Site 0.0016 0 0.0015 0.002 0.0012 0.0001 0.0013 0 0.0002 0 0.0017 0 0.001 0.001 0.0005 0.001 0.0004 0.0001
Cell Periphery 0.0004 0 0.0002 0 0.0016 0.0001 0.0002 0 0 0 0.0002 0 0.0003 0.0001 0.0001 0 0.0003 0.0001
Cytoplasm 0.0047 0 0.0003 0.008 0.0031 0 0.003 0 0.0004 0 0.0003 0.0002 0.0009 0.0003 0.0005 0.0004 0.0001 0
Cytoplasmic Foci 0.0153 0 0.0077 0.0043 0.0212 0.0006 0.0041 0 0.0006 0 0.001 0.0001 0.0092 0.0004 0.0058 0.0052 0.0048 0.0005
Eisosomes 0.0004 0 0.0003 0.0001 0.0002 0 0.0001 0 0 0 0.0003 0 0.0004 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0024 0 0.0002 0.0087 0.0036 0.0001 0.0015 0 0.0005 0 0.0007 0 0.0019 0.0005 0.0004 0.0005 0.0001 0.0001
Endosome 0.0118 0 0.0013 0.0073 0.0232 0.0003 0.0135 0 0.0047 0 0.0035 0.0002 0.0054 0.0035 0.0043 0.0052 0.0023 0.0018
Golgi 0.0041 0 0.002 0.0038 0.0043 0 0.0041 0 0.0038 0 0.0031 0 0.0032 0.0013 0.0014 0.0032 0.0067 0.001
Lipid Particles 0.0079 0 0.0085 0.0009 0.023 0.0001 0.0029 0 0.007 0 0.0027 0.0001 0.0062 0.0003 0.0023 0.0031 0.0037 0.0043
Mitochondria 0.0107 0.0002 0.0068 0.0027 0.0102 0.0012 0.007 0.0002 0.0077 0.0046 0.0096 0.0002 0.0148 0.0033 0.0053 0.0186 0.0048 0.0008
None 0.0103 0 0.0018 0.0013 0.0037 0.0001 0.0161 0 0.0002 0 0.0006 0.0001 0.0011 0.0001 0.0005 0.0006 0.0002 0
Nuclear Periphery 0.0091 0.0003 0.0005 0.0103 0.0263 0.0024 0.0202 0.0002 0.0106 0.0001 0.0556 0.0029 0.0087 0.0025 0.0076 0.0029 0.0008 0.0005
Nucleolus 0.1492 0.0621 0.0556 0.0664 0.4101 0.2383 0.0959 0.0486 0.0277 0.0692 0.3804 0.2013 0.126 0.0556 0.0631 0.1158 0.4397 0.2192
Nucleus 0.7259 0.9372 0.8833 0.8514 0.4245 0.7548 0.7745 0.9509 0.9302 0.9253 0.492 0.7835 0.7677 0.9268 0.8868 0.7726 0.5012 0.7691
Peroxisomes 0.0049 0 0.0053 0.0024 0.0018 0 0.0021 0 0.0004 0 0.0006 0 0.0041 0.0001 0.0004 0.002 0.0105 0.0008
Punctate Nuclear 0.0137 0.0001 0.0036 0.0009 0.0216 0.0002 0.0354 0 0.0006 0 0.0108 0.0105 0.0335 0.0003 0.0168 0.0589 0.0013 0.0006
Vacuole 0.0023 0 0.0004 0.0003 0.004 0.0004 0.0017 0 0.0005 0.0001 0.0007 0.0001 0.0007 0.0006 0.0005 0.0003 0.0003 0.0001
Vacuole Periphery 0.0017 0 0.0004 0.0004 0.0043 0.0007 0.002 0 0.0013 0.0005 0.0038 0 0.0015 0.0008 0.001 0.0005 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 78.8146 76.2416 63.6464 63.5903 64.1751 62.4079 75.8833 78.0436 67.6544 67.5287
Translational Efficiency 1.3711 1.6409 1.3413 1.3479 1.365 1.3648 1.5589 1.2182 1.2343 1.2716

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2479 1339 1448 1335 1596 1523 166 165 4075 2862 1614 1500

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1242.81 1945.14 2116.35 2791.21 1610.05 1757.47 2641.68 3102.26 1386.64 1845.27 2170.38 2825.43
Standard Deviation 280.62 601.39 664.54 829.07 439.69 534.60 548.24 752.21 394.66 574.51 672.73 826.72
Intensity Change Log 2 — 0.646268 0.767972 1.167285 — 0.126395 0.714350 0.946214 — 0.376134 0.737955 1.046708

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000559 0.000578 0.000563 0.000872 0.000511 0.000531 0.000959 0.000863 0.000540 0.000553 0.000603 0.000871
Bud Neck 0.001929 0.002819 0.002855 0.006841 0.002138 0.002062 0.006596 0.006950 0.002011 0.002416 0.003240 0.006853
Bud Site 0.000427 0.000613 0.000422 0.001452 0.000425 0.000436 0.000989 0.000989 0.000426 0.000519 0.000480 0.001401
Cell Periphery 0.000124 0.000031 0.000020 0.000157 0.000032 0.000142 0.000024 0.000044 0.000088 0.000090 0.000020 0.000145
Cytoplasm 0.000163 0.000071 0.000021 0.000255 0.000030 0.000265 0.000025 0.000428 0.000111 0.000174 0.000022 0.000274
Cytoplasmic Foci 0.000250 0.000816 0.000540 0.001290 0.000198 0.001293 0.001082 0.000773 0.000229 0.001070 0.000596 0.001233
Eisosomes 0.000419 0.000309 0.000262 0.000175 0.000336 0.000289 0.000318 0.000138 0.000386 0.000298 0.000268 0.000171
Endoplasmic Reticulum 0.000568 0.000137 0.000097 0.000148 0.000186 0.000205 0.000232 0.000111 0.000418 0.000173 0.000111 0.000144
Endosome 0.000051 0.000213 0.000002 0.000236 0.000005 0.000033 0.000005 0.000007 0.000033 0.000118 0.000003 0.000211
Golgi 0.000267 0.000103 0.000025 0.000213 0.000021 0.000118 0.000069 0.000097 0.000171 0.000111 0.000030 0.000200
Lipid Particles 0.000037 0.000051 0.000002 0.000034 0.000006 0.000867 0.000008 0.000004 0.000025 0.000485 0.000002 0.000030
Mitochondria 0.000872 0.001062 0.001182 0.002421 0.000763 0.000888 0.002133 0.002318 0.000829 0.000969 0.001280 0.002409
Mitotic Spindle 0.000215 0.001313 0.000022 0.002080 0.000016 0.001194 0.000107 0.000938 0.000137 0.001250 0.000031 0.001955
None 0.001358 0.000746 0.000635 0.001528 0.000621 0.000959 0.000580 0.001500 0.001069 0.000859 0.000629 0.001524
Nuclear Periphery 0.000018 0.000015 0.000012 0.000215 0.000010 0.000023 0.000027 0.000101 0.000015 0.000019 0.000013 0.000203
Nuclear Periphery Foci 0.000097 0.000087 0.000003 0.000505 0.000055 0.000298 0.000002 0.000012 0.000081 0.000200 0.000003 0.000451
Nucleolus 0.076633 0.100299 0.097133 0.085924 0.081618 0.076677 0.079990 0.105956 0.078585 0.087729 0.095370 0.088127
Nucleus 0.911956* 0.876551* 0.878366* 0.880813* 0.903630* 0.905531* 0.871070* 0.860440* 0.908695* 0.891973* 0.877616* 0.878572*
Peroxisomes 0.002920 0.013355 0.017344 0.013191 0.008939 0.007292 0.035300 0.017891 0.005277 0.010129 0.019191 0.013708
Vacuole 0.000705 0.000430 0.000153 0.000840 0.000187 0.000566 0.000276 0.000153 0.000502 0.000502 0.000166 0.000764
Vacuole Periphery 0.000432 0.000400 0.000339 0.000811 0.000274 0.000331 0.000206 0.000287 0.000370 0.000363 0.000326 0.000754

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.39 0.02 -5.37 -7.31 -10.52 -0.66 -9.86 -7.13 -5.92 0.83 -0.40 -1.74 -8.37 -9.83 -9.94
Bud Neck -2.69 -5.22 -9.22 -6.39 -7.78 0.62 -8.38 -6.30 -6.40 -1.41 -2.22 -8.40 -10.34 -9.05 -7.94
Bud Site -1.32 0.39 -3.26 -2.05 -3.46 0.33 -1.83 -2.16 -3.26 -1.05 -0.66 0.04 -3.31 -2.98 -3.55
Cell Periphery 2.11 2.58 -0.66 -2.86 -3.32 -1.45 1.13 -1.80 1.11 -2.58 -0.07 2.74 -1.39 -1.08 -3.41
Cytoplasm 1.04 2.40 -1.26 -2.67 -4.91 -1.53 0.61 -2.26 -0.77 -2.36 -0.83 2.51 -2.77 -0.91 -5.45
Cytoplasmic Foci -2.02 -2.17 -3.37 -1.19 -2.73 -2.09 -4.51 -3.37 0.88 0.83 -2.69 -4.07 -3.76 -0.38 -2.64
Eisosomes 3.50 5.53 8.37 6.13 5.23 1.56 0.40 9.64 5.41 7.53 3.80 5.89 10.42 6.54 6.46
Endoplasmic Reticulum 5.69 6.31 5.51 -1.05 -5.81 -0.55 -3.07 4.12 1.92 6.16 4.80 6.70 5.75 1.03 -4.42
Endosome -0.85 2.91 -1.09 -0.04 -1.44 -2.01 -0.12 -1.73 1.15 -1.75 -0.94 2.98 -1.20 -0.51 -1.45
Golgi 0.80 1.25 0.28 -0.94 -1.98 -1.56 -4.93 -3.72 -0.27 -2.06 0.45 1.19 -0.20 -0.92 -2.07
Lipid Particles -0.26 1.98 0.41 0.59 -1.65 -1.33 -0.08 -0.24 1.33 -0.31 -1.32 2.06 -0.01 1.32 -1.66
Mitochondria -1.25 -1.96 -8.68 -11.98 -11.45 -2.48 -11.13 -7.81 -7.15 -1.44 -1.47 -4.48 -12.38 -14.78 -11.45
Mitotic Spindle -1.27 1.24 -2.44 -0.81 -2.81 -1.78 -1.93 -1.22 -0.69 -1.16 -2.05 1.10 -2.83 -1.17 -3.04
None 1.40 1.71 -0.29 -1.90 -2.24 -1.76 0.13 -3.20 -2.05 -3.57 0.74 1.64 -1.07 -1.85 -2.55
Nuclear Periphery 0.45 1.38 -2.35 -2.39 -2.45 -2.94 -5.37 -2.33 -2.18 -2.10 -0.98 0.64 -2.66 -2.61 -2.69
Nuclear Periphery Foci 0.36 1.78 -0.82 -0.91 -1.13 -1.02 0.99 0.35 1.15 -1.29 -0.77 2.04 -0.87 -0.55 -1.14
Nucleolus -5.07 -4.88 -2.44 2.09 1.67 1.10 -0.27 -2.45 -2.82 -1.89 -2.90 -4.61 -2.86 -0.71 0.96
Nucleus 6.52 6.75 5.90 -0.14 0.18 -0.32 2.91 3.60 3.68 0.93 4.59 7.04 6.46 3.07 0.50
Peroxisomes -8.96 -11.77 -10.07 -0.21 2.34 1.86 -5.04 -3.64 -4.24 2.82 -7.11 -11.16 -8.82 -3.59 3.23
Vacuole 1.34 4.61 -0.23 -0.95 -1.93 -1.55 -1.98 -0.23 1.50 1.67 0.10 4.60 -0.72 -0.72 -1.91
Vacuole Periphery 0.53 1.72 -0.96 -1.04 -1.22 -1.50 2.43 -1.61 -0.00 -3.49 0.18 1.06 -1.12 -1.14 -1.25
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Nucleotide exchange factor for Gsp1p; localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p; human homolog of the RAN GEF, RCC1, can complement a temperature sensitive point mutant
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Srm1

Srm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Srm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available