Standard name
Human Ortholog
Description Nucleotide exchange factor for Gsp1p; localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p; human homolog of the RAN GEF, RCC1, can complement a temperature sensitive point mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0.05 0.06 0 0 0.06 0.07 0 0.05 0 0.05 0.06 0.09 0 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.07 0.06 0 0 0.06 0.08 0.07 0.11 0.09 0.1 0.11 0 0 0 0.15 0.22 0.06 0 0 0 0 0 0
Nucleus 0.88 0.8 0.73 0.81 0.85 0.78 0.71 0.7 0.64 0.68 0.61 0.67 0.81 0.75 0.7 0.87 0.76 0.82 0.72 0.83 0.61 0.54 0.52 0.49
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.64 0.65 0.6 0.45 0.31 0.45 0.52 0.49 0.61 0.62 0.67 0.66 0.6 0.56 0.48 0.46 0.42 0.45 0.24 0.11 0.27 0.41 0.4 0.38
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 1 2 3
Bud Neck 0 0 0 0 0 1 1 1 3 2 1 0 0 0 0 0 0 0 1 0 0 0 0 2
Bud Site 1 0 0 0 0 0 4 2 2 5 3 2 1 4 5 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 0 9 8 14 8 12 23 23 15 13 8 10 28 46 7 8 5 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 1 0 0 0 1 0 0
Endosome 0 0 0 0 0 0 0 1 0 0 1 0 0 3 4 1 0 0 0 0 0 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Mitochondria 13 3 8 5 5 13 33 28 37 35 25 24 3 5 4 31 33 9 0 0 2 1 0 2
Nucleus 180 32 94 129 202 181 306 277 220 256 161 142 177 355 342 182 112 119 147 58 80 100 176 203
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 132 26 77 72 74 105 225 195 208 234 177 139 130 265 232 96 62 66 48 7 35 76 136 158
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 4 7 1 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 1 10 19 0 1 1 0 0 0 0 3 6
Vac/Vac Membrane 1 1 0 0 1 0 2 1 2 3 0 0 1 4 5 1 3 0 2 2 2 3 15 33
Unique Cell Count 205 40 128 159 238 231 433 397 342 376 263 211 218 472 487 209 148 146 204 70 132 186 339 418
Labelled Cell Count 330 62 188 214 297 309 583 531 495 550 381 315 323 679 664 320 220 202 204 70 132 186 339 418


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.7 20.0 19.6 21.4 21.9 18.5 16.8 16.5 18.0 16.4 16.7 17.3 25.9 26.5 25.2 39.4 40.2 43.8 24.4 22.8 23.9
Std Deviation (1e-4) 8.6 7.1 9.7 8.2 9.6 8.1 7.7 7.0 9.7 8.9 9.6 9.0 9.3 11.5 13.5 18.0 20.8 22.8 9.6 7.7 9.5
Intensity Change (Log2) – – – 0.13 0.16 -0.08 -0.23 -0.25 -0.12 -0.26 -0.24 -0.18 0.4 0.43 0.36 1.01 1.03 1.16 0.31 0.22 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 -0.4 -1.5 0 -0.5 -0.1 -1.4 -0.8 -1.3 -1.0 -0.5 0.9 -1.5 -0.6 -1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.3 0 -0.2 0.5 0.3 1.5 1.1 1.1 1.6 0 0 0 2.4 3.7 0
Nucleus 1.6 2.7 1.1 -0.6 -0.8 -1.9 -1.1 -2.4 -1.2 1.7 0.4 -0.7 3.2 0.4 1.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.5 -5.4 -2.7 -1.6 -2.2 0.1 0.4 1.4 1.1 -0.1 -0.8 -2.5 -2.5 -3.0 -2.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.3908 22.6727 20.6937 22.9747 17.0677 21.4486 21.0814 20.1817 22.0225 22.4883 18.6424 18.4352 31.6632 27.421 24.2836 24.5127 26.043 24.8273
Actin 0.0222 0 0.019 0.0281 0.0099 0.0002 0.0122 0 0.0031 0 0.0302 0.0002 0.012 0.0019 0.0024 0.0084 0.0216 0.0007
Bud 0.0004 0 0.0003 0.0003 0.0006 0.0001 0.0009 0 0.0001 0 0.0007 0 0.0003 0.0003 0.0002 0.0003 0.0002 0
Bud Neck 0.0006 0 0.0004 0.0001 0.0008 0.0001 0.0003 0 0 0 0.0003 0.0004 0.0002 0 0.0001 0.0001 0.0002 0.0001
Bud Periphery 0.0006 0 0.0004 0.0002 0.0008 0.0002 0.0011 0 0.0002 0 0.0012 0 0.0008 0.0003 0.0002 0.0003 0.0003 0
Bud Site 0.0016 0 0.0015 0.002 0.0012 0.0001 0.0013 0 0.0002 0 0.0017 0 0.001 0.001 0.0005 0.001 0.0004 0.0001
Cell Periphery 0.0004 0 0.0002 0 0.0016 0.0001 0.0002 0 0 0 0.0002 0 0.0003 0.0001 0.0001 0 0.0003 0.0001
Cytoplasm 0.0047 0 0.0003 0.008 0.0031 0 0.003 0 0.0004 0 0.0003 0.0002 0.0009 0.0003 0.0005 0.0004 0.0001 0
Cytoplasmic Foci 0.0153 0 0.0077 0.0043 0.0212 0.0006 0.0041 0 0.0006 0 0.001 0.0001 0.0092 0.0004 0.0058 0.0052 0.0048 0.0005
Eisosomes 0.0004 0 0.0003 0.0001 0.0002 0 0.0001 0 0 0 0.0003 0 0.0004 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0024 0 0.0002 0.0087 0.0036 0.0001 0.0015 0 0.0005 0 0.0007 0 0.0019 0.0005 0.0004 0.0005 0.0001 0.0001
Endosome 0.0118 0 0.0013 0.0073 0.0232 0.0003 0.0135 0 0.0047 0 0.0035 0.0002 0.0054 0.0035 0.0043 0.0052 0.0023 0.0018
Golgi 0.0041 0 0.002 0.0038 0.0043 0 0.0041 0 0.0038 0 0.0031 0 0.0032 0.0013 0.0014 0.0032 0.0067 0.001
Lipid Particles 0.0079 0 0.0085 0.0009 0.023 0.0001 0.0029 0 0.007 0 0.0027 0.0001 0.0062 0.0003 0.0023 0.0031 0.0037 0.0043
Mitochondria 0.0107 0.0002 0.0068 0.0027 0.0102 0.0012 0.007 0.0002 0.0077 0.0046 0.0096 0.0002 0.0148 0.0033 0.0053 0.0186 0.0048 0.0008
None 0.0103 0 0.0018 0.0013 0.0037 0.0001 0.0161 0 0.0002 0 0.0006 0.0001 0.0011 0.0001 0.0005 0.0006 0.0002 0
Nuclear Periphery 0.0091 0.0003 0.0005 0.0103 0.0263 0.0024 0.0202 0.0002 0.0106 0.0001 0.0556 0.0029 0.0087 0.0025 0.0076 0.0029 0.0008 0.0005
Nucleolus 0.1492 0.0621 0.0556 0.0664 0.4101 0.2383 0.0959 0.0486 0.0277 0.0692 0.3804 0.2013 0.126 0.0556 0.0631 0.1158 0.4397 0.2192
Nucleus 0.7259 0.9372 0.8833 0.8514 0.4245 0.7548 0.7745 0.9509 0.9302 0.9253 0.492 0.7835 0.7677 0.9268 0.8868 0.7726 0.5012 0.7691
Peroxisomes 0.0049 0 0.0053 0.0024 0.0018 0 0.0021 0 0.0004 0 0.0006 0 0.0041 0.0001 0.0004 0.002 0.0105 0.0008
Punctate Nuclear 0.0137 0.0001 0.0036 0.0009 0.0216 0.0002 0.0354 0 0.0006 0 0.0108 0.0105 0.0335 0.0003 0.0168 0.0589 0.0013 0.0006
Vacuole 0.0023 0 0.0004 0.0003 0.004 0.0004 0.0017 0 0.0005 0.0001 0.0007 0.0001 0.0007 0.0006 0.0005 0.0003 0.0003 0.0001
Vacuole Periphery 0.0017 0 0.0004 0.0004 0.0043 0.0007 0.002 0 0.0013 0.0005 0.0038 0 0.0015 0.0008 0.001 0.0005 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 78.8146 76.2416 63.6464 63.5903 64.1751 62.4079 75.8833 78.0436 67.6544 67.5287
Translational Efficiency 1.3711 1.6409 1.3413 1.3479 1.365 1.3648 1.5589 1.2182 1.2343 1.2716

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nucleotide exchange factor for Gsp1p; localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p; human homolog of the RAN GEF, RCC1, can complement a temperature sensitive point mutant
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Srm1

Srm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Srm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available