Standard name | |
---|---|
Human Ortholog | |
Description | Essential SNARE protein localized to the ER; involved in retrograde traffic from the Golgi to the ER and Sey1p-independent homotypic ER fusion; required for efficient nuclear fusion during mating; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 3 | 14 | 7 | 8 | 2 | 2 | 0 | 1 | 1 | 2 | 3 | 21 | 23 | 31 | 16 | 37 | 36 | 5 | 10 | 16 | 18 | 40 | 43 |
Bud | – | 1 | 4 | 3 | 8 | 8 | 11 | 15 | 19 | 18 | 9 | 18 | 5 | 6 | 5 | 5 | 12 | 11 | 0 | 0 | 0 | 1 | 3 | 4 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 5 | 0 | 0 | 0 | 1 | 3 | 4 | 7 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 4 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 4 | 7 | 5 | 9 | 7 | 2 | 5 | 5 | 6 | 13 | 1 | 6 | 7 | 7 | 12 | 9 | 0 | 1 | 1 | 1 | 2 | 4 |
Cytoplasm | – | 23 | 21 | 85 | 126 | 82 | 158 | 98 | 114 | 104 | 83 | 107 | 28 | 63 | 74 | 46 | 72 | 62 | 2 | 5 | 11 | 8 | 6 | 13 |
Endoplasmic Reticulum | – | 30 | 40 | 30 | 48 | 40 | 47 | 16 | 18 | 30 | 10 | 33 | 91 | 113 | 143 | 6 | 15 | 12 | 20 | 50 | 52 | 47 | 82 | 110 |
Endosome | – | 0 | 0 | 1 | 13 | 2 | 6 | 12 | 9 | 9 | 7 | 1 | 0 | 11 | 5 | 10 | 10 | 8 | 0 | 0 | 1 | 2 | 7 | 9 |
Golgi | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 3 | 0 | 1 | 1 | 5 | 8 | 5 |
Mitochondria | – | 5 | 30 | 61 | 82 | 135 | 306 | 208 | 177 | 259 | 155 | 285 | 1 | 0 | 1 | 12 | 26 | 11 | 7 | 4 | 22 | 29 | 48 | 35 |
Nucleus | – | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 0 | 1 | 0 | 1 | 2 | 4 | 0 | 2 | 1 | 2 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 3 | 0 | 0 | 3 | 1 | 0 | 2 | 0 | 0 | 2 | 0 | 2 | 3 | 5 | 0 | 0 | 1 | 1 | 1 | 0 |
SpindlePole | – | 0 | 0 | 0 | 2 | 0 | 1 | 6 | 1 | 5 | 0 | 1 | 1 | 3 | 1 | 5 | 6 | 10 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 0 | 1 | 0 | 3 | 8 | 28 | 27 | 23 | 37 | 13 | 8 | 2 | 6 | 2 | 4 | 7 | 13 | 0 | 2 | 3 | 8 | 9 | 17 |
Unique Cell Count | 55 | 89 | 164 | 256 | 231 | 453 | 323 | 287 | 377 | 229 | 370 | 133 | 208 | 238 | 98 | 162 | 153 | 38 | 78 | 116 | 131 | 221 | 256 | |
Labelled Cell Count | 62 | 115 | 196 | 299 | 289 | 572 | 391 | 373 | 476 | 291 | 475 | 150 | 237 | 271 | 120 | 207 | 182 | 38 | 78 | 116 | 131 | 221 | 256 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 5.9 | 4.4 | 4.9 | 4.8 | 4.3 | 4.0 | 4.3 | 4.0 | 4.1 | 4.0 | 4.0 | 6.5 | 6.7 | 6.7 | 6.5 | 6.5 | 6.6 | 7.2 | 7.5 | 7.7 |
Std Deviation (1e-4) | – | 0.6 | 0.6 | 1.4 | 1.2 | 1.1 | 1.4 | 1.1 | 1.0 | 0.9 | 1.5 | 0.8 | 0.8 | 0.9 | 0.8 | 1.3 | 1.4 | 1.4 | 1.3 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.13 | 0.12 | -0.03 | -0.15 | -0.05 | -0.16 | -0.13 | -0.16 | -0.16 | 0.56 | 0.6 | 0.61 | 0.55 | 0.55 | 0.57 | 0.7 | 0.76 | 0.8 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.1 | -0.6 | 0.1 | 1.3 | 1.4 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 0.8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0.9 | 0 |
Cytoplasm | 4.3 | 4.2 | 2.0 | 2.1 | 1.2 | 2.8 | 0.8 | 2.2 | 1.0 | -0.4 | 1.2 | 1.3 | 3.3 | 3.3 | 2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 1.5 | 2.5 | -6.2 | -6.5 | -6.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.9 | -8.8 | -9.1 | -3.5 | -3.2 | -5.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 2.4 | 2.3 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.6849 | 3.0432 | 2.9193 | 2.69 | 2.595 | 2.8793 | 3.7141 | 4.4553 | 4.0081 | 3.0626 | 3.5662 | 4.0233 | 2.3425 | 3.5083 | 2.5406 | 2.8238 | 2.504 | 2.8372 |
Actin | 0.0345 | 0.0029 | 0.034 | 0.0058 | 0.068 | 0.003 | 0.0671 | 0.0029 | 0.0276 | 0.037 | 0.0469 | 0.0051 | 0.0364 | 0.0054 | 0.0234 | 0.0116 | 0.0013 | 0.0064 |
Bud | 0.0005 | 0.0006 | 0.0007 | 0.0004 | 0.0004 | 0.0001 | 0.0004 | 0.0003 | 0.0041 | 0.0095 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0001 |
Bud Neck | 0.001 | 0.0028 | 0.0066 | 0.0021 | 0.0005 | 0.0033 | 0.0031 | 0.0008 | 0.0015 | 0.0004 | 0.001 | 0.0022 | 0.004 | 0.0013 | 0.003 | 0.0011 | 0.0016 | 0.0069 |
Bud Periphery | 0.0009 | 0.0013 | 0.0007 | 0.0003 | 0.0006 | 0.0004 | 0.0007 | 0.0005 | 0.004 | 0.016 | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0005 | 0.0009 | 0.0001 | 0.0001 |
Bud Site | 0.0036 | 0.0096 | 0.0123 | 0.0162 | 0.0009 | 0.0006 | 0.0076 | 0.0207 | 0.0151 | 0.0026 | 0.0009 | 0.0004 | 0.0023 | 0.0052 | 0.0033 | 0.001 | 0.0007 | 0.0002 |
Cell Periphery | 0.0005 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0046 | 0.0009 | 0.001 | 0.0005 | 0.0002 | 0.0003 | 0.0003 | 0.0008 | 0.0003 | 0.0006 | 0.0005 | 0.0001 |
Cytoplasm | 0.002 | 0.0043 | 0.0027 | 0.0027 | 0.0019 | 0.0017 | 0.0289 | 0.0402 | 0.0108 | 0.0088 | 0.0223 | 0.0029 | 0.0066 | 0.0137 | 0.002 | 0.0017 | 0.0012 | 0.0011 |
Cytoplasmic Foci | 0.169 | 0.1753 | 0.1449 | 0.1598 | 0.0879 | 0.0928 | 0.1175 | 0.0976 | 0.1235 | 0.1173 | 0.0859 | 0.0816 | 0.1018 | 0.1369 | 0.1132 | 0.0994 | 0.1196 | 0.0836 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0002 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0047 | 0.0034 | 0.0073 | 0.0006 | 0.002 | 0.0041 | 0.0092 | 0.016 | 0.0186 | 0.0191 | 0.0297 | 0.011 | 0.0129 | 0.0159 | 0.0163 | 0.0023 | 0.003 | 0.0035 |
Endosome | 0.1138 | 0.1303 | 0.1055 | 0.1166 | 0.1777 | 0.0687 | 0.0918 | 0.1115 | 0.104 | 0.1227 | 0.1379 | 0.0568 | 0.0896 | 0.1069 | 0.1087 | 0.146 | 0.0864 | 0.0534 |
Golgi | 0.0469 | 0.0483 | 0.0684 | 0.0709 | 0.0832 | 0.0583 | 0.0437 | 0.0519 | 0.0774 | 0.093 | 0.0944 | 0.0511 | 0.0475 | 0.0571 | 0.0831 | 0.0899 | 0.0489 | 0.049 |
Lipid Particles | 0.5197 | 0.5197 | 0.4747 | 0.4717 | 0.538 | 0.6603 | 0.4382 | 0.5543 | 0.5215 | 0.5073 | 0.4893 | 0.7078 | 0.6162 | 0.581 | 0.5736 | 0.5873 | 0.6403 | 0.7478 |
Mitochondria | 0.0193 | 0.0046 | 0.0218 | 0.0124 | 0.0067 | 0.0162 | 0.0147 | 0.0343 | 0.0116 | 0.0255 | 0.0093 | 0.0049 | 0.0053 | 0.0076 | 0.008 | 0.013 | 0.0044 | 0.0089 |
None | 0.0005 | 0.0008 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0152 | 0.0259 | 0.0086 | 0.0003 | 0.0013 | 0.0047 | 0.0083 | 0.0011 | 0.001 | 0.0018 | 0.0005 | 0.0002 |
Nuclear Periphery | 0.0087 | 0.0022 | 0.0059 | 0.0003 | 0.0005 | 0.0042 | 0.0157 | 0.0019 | 0.0032 | 0.0011 | 0.009 | 0.0061 | 0.0111 | 0.0036 | 0.0063 | 0.0013 | 0.0101 | 0.0016 |
Nucleolus | 0.0013 | 0.0123 | 0.0177 | 0.0008 | 0.0001 | 0.0082 | 0.0033 | 0.0003 | 0.0007 | 0.0003 | 0.0005 | 0.0058 | 0.0011 | 0.0098 | 0.0035 | 0.001 | 0.0153 | 0.0003 |
Nucleus | 0.0148 | 0.0016 | 0.0015 | 0.0005 | 0.0001 | 0.0064 | 0.0062 | 0.0004 | 0.0007 | 0.0002 | 0.0022 | 0.0016 | 0.0009 | 0.0009 | 0.0013 | 0.0004 | 0.0019 | 0.0001 |
Peroxisomes | 0.034 | 0.0456 | 0.0594 | 0.1314 | 0.0235 | 0.057 | 0.0599 | 0.0299 | 0.0444 | 0.0289 | 0.0539 | 0.0414 | 0.0337 | 0.0322 | 0.0326 | 0.0253 | 0.0267 | 0.025 |
Punctate Nuclear | 0.0192 | 0.0248 | 0.0288 | 0.0029 | 0.0019 | 0.009 | 0.0595 | 0.002 | 0.0111 | 0.0029 | 0.0085 | 0.0125 | 0.0162 | 0.0086 | 0.0128 | 0.0063 | 0.0287 | 0.0095 |
Vacuole | 0.0028 | 0.0078 | 0.005 | 0.0033 | 0.0039 | 0.0034 | 0.01 | 0.0056 | 0.0083 | 0.0042 | 0.0038 | 0.0023 | 0.0028 | 0.0101 | 0.0045 | 0.0069 | 0.0066 | 0.0013 |
Vacuole Periphery | 0.0021 | 0.0013 | 0.0015 | 0.0009 | 0.0017 | 0.0017 | 0.0018 | 0.0018 | 0.0018 | 0.0023 | 0.0023 | 0.0013 | 0.0014 | 0.0015 | 0.0021 | 0.0016 | 0.002 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.4562 | 9.3559 | 5.9507 | 12.425 | 11.631 | 8.9658 | 17.568 | 12.3133 | 20.4726 | 13.513 |
Translational Efficiency | 1.1084 | 1.6775 | 2.6918 | 1.2174 | 1.1005 | 2.3411 | 1.2565 | 1.6466 | 1.2034 | 1.5153 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2222 | 1703 | 2250 | 993 | 1418 | 105 | 1869 | 381 | 3640 | 1808 | 4119 | 1374 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 750.95 | 918.39 | 1094.74 | 1055.35 | 801.15 | 874.79 | 1051.34 | 1121.29 | 770.51 | 915.86 | 1075.05 | 1073.63 |
Standard Deviation | 89.78 | 126.80 | 139.46 | 149.73 | 106.20 | 124.47 | 134.45 | 160.70 | 99.56 | 127.08 | 138.90 | 155.68 |
Intensity Change Log 2 | — | 0.290390 | 0.543800 | 0.490933 | — | 0.126864 | 0.392085 | 0.485015 | — | 0.208299 | 0.467482 | 0.487881 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.004779 | 0.017216 | 0.063576 | 0.049642 | 0.008519 | 0.015749 | 0.056584 | 0.053964 | 0.006236 | 0.017131 | 0.060403 | 0.050841 |
Bud Neck | 0.006014 | 0.010014 | 0.014854 | 0.014161 | 0.007356 | 0.006512 | 0.005832 | 0.013377 | 0.006537 | 0.009811 | 0.010760 | 0.013944 |
Bud Site | 0.004253 | 0.028855 | 0.039268 | 0.080598 | 0.008303 | 0.012507 | 0.019578 | 0.070918 | 0.005831 | 0.027905 | 0.030333 | 0.077914 |
Cell Periphery | 0.001232 | 0.003697 | 0.002981 | 0.002644 | 0.002498 | 0.009754 | 0.001249 | 0.003292 | 0.001725 | 0.004049 | 0.002195 | 0.002824 |
Cytoplasm | 0.199549* | 0.161790* | 0.089874 | 0.082763 | 0.177729* | 0.101455* | 0.031339 | 0.057257 | 0.191049* | 0.158286* | 0.063313 | 0.075690 |
Cytoplasmic Foci | 0.134623 | 0.125930 | 0.032736 | 0.095287 | 0.138789 | 0.100045 | 0.064167 | 0.072925 | 0.136246 | 0.124427 | 0.046998 | 0.089086 |
Eisosomes | 0.007172 | 0.006588 | 0.003592 | 0.002385 | 0.006067 | 0.008660 | 0.001532 | 0.003213 | 0.006742 | 0.006709 | 0.002657 | 0.002615 |
Endoplasmic Reticulum | 0.140836* | 0.229354* | 0.394229* | 0.234013* | 0.209871* | 0.172549* | 0.244262* | 0.313161* | 0.167729* | 0.226055* | 0.326181* | 0.255961* |
Endosome | 0.022162 | 0.031900 | 0.038972 | 0.063794 | 0.023246 | 0.048278 | 0.092335 | 0.069466 | 0.022585 | 0.032852 | 0.063186 | 0.065367 |
Golgi | 0.156471* | 0.148816* | 0.037481 | 0.030872 | 0.160952* | 0.188408* | 0.058390 | 0.039696 | 0.158217* | 0.151115* | 0.046969 | 0.033318 |
Lipid Particles | 0.217773* | 0.135578* | 0.212021* | 0.134971* | 0.169010* | 0.234440* | 0.352837* | 0.160976* | 0.198777* | 0.141319* | 0.275916* | 0.142182* |
Mitochondria | 0.033394 | 0.030525 | 0.007219 | 0.004491 | 0.025539 | 0.020882 | 0.003633 | 0.002067 | 0.030334 | 0.029965 | 0.005592 | 0.003819 |
Mitotic Spindle | 0.000198 | 0.001224 | 0.004736 | 0.036644 | 0.000208 | 0.000418 | 0.010670 | 0.011134 | 0.000202 | 0.001177 | 0.007429 | 0.029570 |
None | 0.004178 | 0.002028 | 0.003676 | 0.000224 | 0.001766 | 0.000158 | 0.000947 | 0.000067 | 0.003238 | 0.001920 | 0.002438 | 0.000181 |
Nuclear Periphery | 0.000522 | 0.000222 | 0.000920 | 0.001800 | 0.000428 | 0.000334 | 0.001285 | 0.000665 | 0.000485 | 0.000229 | 0.001086 | 0.001485 |
Nuclear Periphery Foci | 0.001295 | 0.002258 | 0.016352 | 0.032014 | 0.002490 | 0.001775 | 0.023132 | 0.019118 | 0.001761 | 0.002230 | 0.019428 | 0.028438 |
Nucleolus | 0.000297 | 0.000241 | 0.000165 | 0.000536 | 0.000280 | 0.000118 | 0.000245 | 0.000076 | 0.000290 | 0.000233 | 0.000201 | 0.000408 |
Nucleus | 0.016986 | 0.007017 | 0.010568 | 0.010369 | 0.013288 | 0.004292 | 0.002572 | 0.004884 | 0.015545 | 0.006859 | 0.006940 | 0.008848 |
Peroxisomes | 0.034350 | 0.038306 | 0.013817 | 0.011177 | 0.029593 | 0.045041 | 0.011227 | 0.009669 | 0.032497 | 0.038697 | 0.012642 | 0.010759 |
Vacuole | 0.010681 | 0.013222 | 0.012088 | 0.108090 | 0.010886 | 0.022735 | 0.016846 | 0.091368 | 0.010761 | 0.013775 | 0.014247 | 0.103453 |
Vacuole Periphery | 0.003236 | 0.005218 | 0.000878 | 0.003525 | 0.003182 | 0.005890 | 0.001336 | 0.002708 | 0.003215 | 0.005257 | 0.001086 | 0.003298 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -8.90 | -17.92 | -10.32 | -7.01 | 3.13 | -1.36 | -13.23 | -6.26 | -4.24 | 0.44 | -8.16 | -22.28 | -11.94 | -8.45 | 2.69 |
Bud Neck | -3.54 | -7.15 | -6.70 | -2.92 | 0.41 | 0.41 | 1.33 | -2.53 | -2.41 | -3.32 | -3.09 | -5.26 | -6.69 | -3.12 | -2.68 |
Bud Site | -11.01 | -15.83 | -14.82 | -9.24 | -7.27 | -1.07 | -6.49 | -7.87 | -6.61 | -6.30 | -10.44 | -16.95 | -16.65 | -10.40 | -10.36 |
Cell Periphery | -6.83 | -7.67 | -3.53 | 2.46 | 1.20 | -1.66 | 4.27 | -1.58 | 1.50 | -5.67 | -5.34 | -2.70 | -3.26 | 2.76 | -1.68 |
Cytoplasm | 6.75 | 22.98 | 19.01 | 12.40 | 0.61 | 6.02 | 30.06 | 16.98 | 3.30 | -4.67 | 6.72 | 36.98 | 24.13 | 15.00 | -3.84 |
Cytoplasmic Foci | 1.98 | 31.11 | 7.96 | 6.05 | -13.87 | 3.06 | 16.34 | 9.87 | 2.10 | -1.29 | 3.02 | 32.73 | 11.72 | 7.86 | -11.04 |
Eisosomes | 0.93 | 6.80 | 8.45 | 7.97 | 3.08 | -1.30 | 8.01 | 3.95 | 2.81 | -2.95 | 0.06 | 10.59 | 9.27 | 8.44 | 0.53 |
Endoplasmic Reticulum | -11.04 | -30.29 | -7.31 | 0.96 | 14.62 | 1.53 | -3.30 | -4.71 | -4.30 | -2.93 | -7.76 | -24.28 | -7.77 | -1.34 | 8.44 |
Endosome | -6.04 | -9.58 | -13.79 | -9.82 | -7.21 | -3.46 | -21.97 | -8.33 | -2.22 | 4.00 | -6.87 | -22.96 | -16.05 | -10.97 | -0.00 |
Golgi | 1.55 | 34.70* | 32.91* | 27.02 | 3.38 | -1.62 | 22.26 | 20.69 | 8.94 | 4.29 | 1.58 | 40.04 | 39.38 | 28.25 | 6.69 |
Lipid Particles | 13.90 | 0.91 | 11.52 | 0.58 | 9.87 | -3.03 | -21.38 | 0.90 | 3.21 | 15.32 | 11.23 | -13.68 | 9.69 | 0.35 | 19.62 |
Mitochondria | 1.28 | 16.14 | 16.74 | 14.21 | 2.96 | 0.81 | 12.93 | 14.14 | 3.38 | 2.52 | 0.19 | 20.83 | 20.79 | 15.49 | 2.74 |
Mitotic Spindle | -2.85 | -6.20 | -9.21 | -8.94 | -8.00 | -1.41 | -7.96 | -3.53 | -3.47 | -0.09 | -2.90 | -10.04 | -9.78 | -9.42 | -7.25 |
None | 3.19 | 0.64 | 7.52 | 4.18 | 5.13 | 5.33 | 2.22 | 5.70 | 1.91 | 4.01 | 2.51 | 1.63 | 8.93 | 4.30 | 5.91 |
Nuclear Periphery | 3.46 | -3.28 | -4.43 | -5.52 | -3.11 | 0.84 | -3.90 | -1.87 | -2.22 | 2.31 | 4.28 | -4.97 | -4.76 | -5.97 | -1.97 |
Nuclear Periphery Foci | -2.37 | -13.85 | -11.98 | -11.51 | -5.74 | 1.94 | -11.58 | -6.43 | -6.67 | 0.86 | -1.22 | -17.61 | -13.42 | -13.01 | -4.29 |
Nucleolus | 0.74 | 5.29 | -0.82 | -0.94 | -1.15 | 1.93 | 0.13 | 2.48 | 1.51 | 2.01 | 0.75 | 1.43 | -0.62 | -0.80 | -0.89 |
Nucleus | 9.71 | 5.85 | 3.90 | -3.64 | -0.66 | 5.80 | 10.36 | 5.76 | -0.68 | -2.53 | 10.14 | 11.32 | 5.38 | -2.93 | -2.96 |
Peroxisomes | -1.77 | 14.20 | 13.50 | 12.30 | 1.80 | -1.99 | 12.07 | 11.52 | 4.62 | 1.39 | -3.06 | 18.78 | 16.80 | 13.86 | 1.83 |
Vacuole | -2.37 | -2.22 | -19.13 | -18.62 | -18.66 | -1.86 | -4.23 | -9.84 | -7.37 | -9.16 | -2.90 | -4.72 | -21.41 | -20.62 | -20.48 |
Vacuole Periphery | -4.08 | 9.63 | -0.04 | 3.06 | -4.89 | -1.60 | 7.14 | 0.92 | 1.85 | -1.89 | -4.60 | 11.64 | 0.35 | 3.86 | -4.97 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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