Standard name
Human Ortholog
Description Protein of unknown function; non-essential gene; interacts with the DNA helicase Hpr5p; YGL101W has a paralog, YBR242W, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 0.92 0.87 0.79 0.78 – 0.81 0.8 0.75 0.74 0.99 0.99 0.99 0.95 0.98 0.96 0.95 0.93 0.93 0.97 0.92 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.09 0.07 0.1 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.13 0.17 0.22 0.22 – 0.12 0.13 0.14 0.12 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 1 0 0 0 2 5
Bud 0 0 0 0 0 1 0 – 2 0 1 1 1 0 0 0 0 0 1 1 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 1 1 1 4 – 4 3 2 4 0 0 2 3 4 1 0 0 0 0 0 0
Cytoplasm 285 346 140 109 180 159 210 – 139 171 125 128 194 280 285 82 97 77 277 331 136 159 255 212
Endoplasmic Reticulum 2 0 1 0 0 0 0 – 1 3 2 4 1 0 0 4 0 1 2 2 1 0 2 4
Endosome 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 2 1 0 4 24
Golgi 1 2 0 0 0 0 1 – 0 0 0 0 0 0 0 1 1 1 4 1 1 0 2 6
Mitochondria 1 2 0 0 0 6 4 – 16 14 17 24 0 1 0 3 2 1 0 6 1 0 1 0
Nucleus 2 3 1 2 2 2 5 – 4 9 9 5 2 1 0 2 0 3 1 2 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 0 – 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 2
Vac/Vac Membrane 6 2 4 16 36 44 59 – 21 29 23 20 0 2 3 2 1 2 1 5 0 0 1 3
Unique Cell Count 291 351 144 119 208 202 270 172 215 167 172 196 282 287 86 99 80 293 358 146 164 277 268
Labelled Cell Count 298 356 146 128 219 213 283 189 229 180 187 198 284 290 99 105 86 293 358 146 164 277 268


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.1 13.0 11.6 11.4 12.4 8.2 10.4 – 8.7 9.2 8.3 7.8 13.7 11.2 13.6 17.4 17.9 18.5 12.0 12.3 12.5
Std Deviation (1e-4) 2.2 2.4 1.9 1.7 1.9 2.0 2.0 – 1.7 1.5 1.2 1.5 2.1 1.9 2.0 4.3 4.5 3.8 2.1 1.8 1.9
Intensity Change (Log2) – – – -0.02 0.11 -0.5 -0.15 – -0.4 -0.32 -0.48 -0.57 0.24 -0.05 0.24 0.59 0.63 0.68 0.05 0.09 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.0 -3.4 -5.0 -5.2 – -4.5 -4.8 -5.5 -5.6 1.2 1.7 1.7 -0.8 0.4 -0.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 3.8 3.1 3.9 4.7 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 3.2 4.2 5.0 5.1 – 3.1 3.4 3.4 3.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4886 6.5654 5.5393 5.2348 4.3275 6.167 7.4218 8.9508 8.6546 8.828 8.902 9.1606 10.0623 11.5752 10.9505 10.7664 11.0225 10.9015
Actin 0.0216 0.0033 0.0053 0.012 0.0032 0.0031 0.0121 0.0011 0.0169 0.0498 0.002 0.0028 0.0005 0.0006 0.0005 0.0009 0.0003 0.0004
Bud 0.0017 0.0002 0.0006 0.0063 0.0017 0.0001 0.0006 0.0001 0.0012 0.0004 0.0001 0.0001 0.0001 0.0008 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0005 0.0002 0.0004 0.0012 0.0002 0.0009 0.0021 0.0002 0.0015 0.001 0.0008 0.001 0.0002 0.0003 0.0006 0.0005 0.0003 0.0007
Bud Periphery 0.0007 0.0001 0.0003 0.0083 0.002 0.0001 0.0006 0.0001 0.0011 0.0005 0 0 0 0.0005 0 0 0 0
Bud Site 0.0024 0.0005 0.0014 0.0329 0.0012 0.0001 0.0018 0.0005 0.0051 0.0017 0.0002 0 0.0001 0.0005 0.0002 0.0002 0 0
Cell Periphery 0.0001 0 0.0001 0.0004 0.0001 0 0.0002 0 0.0001 0.0001 0 0 0 0 0 0 0 0
Cytoplasm 0.8224 0.9747 0.9424 0.8973 0.7941 0.9471 0.8624 0.9753 0.9402 0.9101 0.9347 0.9663 0.972 0.9717 0.9842 0.9761 0.9894 0.9713
Cytoplasmic Foci 0.0561 0.004 0.007 0.0089 0.0072 0.0069 0.0304 0.0032 0.0084 0.003 0.0061 0.0058 0.0015 0.0031 0.0022 0.0021 0.0015 0.0023
Eisosomes 0.0003 0 0 0.0001 0 0 0.0001 0 0.0001 0.0002 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0032 0.0028 0.0039 0.0011 0.0007 0.001 0.0097 0.0018 0.0014 0.0015 0.0046 0.0023 0.0032 0.0014 0.0019 0.0018 0.0007 0.0054
Endosome 0.0218 0.0031 0.0105 0.0026 0.0253 0.0023 0.0318 0.0022 0.0025 0.0034 0.018 0.0027 0.0006 0.0031 0.0008 0.0008 0.0004 0.0015
Golgi 0.0049 0.0051 0.0033 0.0017 0.0256 0.0037 0.0072 0.0026 0.0013 0.0063 0.0007 0.0003 0 0.0016 0.0001 0.0001 0 0.0001
Lipid Particles 0.0075 0.0001 0.0041 0.001 0.0005 0.0083 0.0022 0.0006 0.0006 0.0011 0.0008 0 0 0 0 0 0 0
Mitochondria 0.0044 0.0013 0.0027 0.0095 0.1283 0.0023 0.0018 0.0032 0.0009 0.0029 0.0003 0.0002 0.0001 0.0087 0.0001 0.0001 0.0001 0.0008
None 0.0094 0.0008 0.0035 0.0022 0.0006 0.001 0.011 0.0021 0.0029 0.0016 0.0053 0.0018 0.0078 0.0016 0.0013 0.0022 0.0011 0.0025
Nuclear Periphery 0.0025 0.0006 0.0025 0.0013 0.0005 0.0009 0.0046 0.0012 0.0015 0.0014 0.009 0.0017 0.002 0.0009 0.0011 0.0016 0.0006 0.0016
Nucleolus 0.0004 0 0.0001 0.0004 0 0 0.0002 0 0.0001 0 0.0002 0 0 0 0 0 0 0
Nucleus 0.0055 0.0017 0.0065 0.0065 0.0048 0.0069 0.0114 0.0042 0.0087 0.0112 0.0105 0.0136 0.0109 0.0042 0.0061 0.0127 0.0049 0.012
Peroxisomes 0.0183 0.0011 0.0014 0.0036 0.0011 0.0144 0.0035 0.0005 0.0038 0.0026 0.0002 0.0004 0 0.0002 0.0001 0.0001 0 0.0003
Punctate Nuclear 0.0132 0.0001 0.0013 0.0011 0.0001 0.0005 0.0038 0.0006 0.0012 0.0006 0.0055 0.0005 0.0004 0.0003 0.0004 0.0006 0.0002 0.0007
Vacuole 0.0027 0.0002 0.0021 0.0013 0.0014 0.0002 0.0018 0.0003 0.0005 0.0003 0.0007 0.0004 0.0003 0.0004 0.0003 0.0002 0.0002 0.0002
Vacuole Periphery 0.0004 0.0001 0.0008 0.0003 0.0014 0.0001 0.0006 0.0001 0.0001 0.0002 0.0004 0.0001 0.0001 0.0002 0.0001 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.4334 45.6656 25.9791 18.1282 30.9083 15.3166 40.7856 26.4888 21.9604 25.4385
Translational Efficiency 2.1796 1.5292 1.2778 1.4111 1.4522 2.345 1.5204 1.6807 1.7858 1.9248

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
332 1430 711 2156 2736 2036 2034 1935 3068 3466 2745 4091

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 909.71 1239.66 1527.16 1537.22 1094.07 1226.32 1424.39 1563.78 1074.12 1231.82 1451.01 1549.78
Standard Deviation 130.23 189.47 192.83 253.15 151.73 177.53 191.93 241.14 160.14 182.67 197.36 247.90
Intensity Change Log 2 — 0.446466 0.747373 0.756845 — 0.164630 0.380639 0.515333 — 0.299437 0.558749 0.630009

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000511 0.000129 0.000405 0.000214 0.000069 0.000263 0.000096 0.001034 0.000117 0.000208 0.000176 0.000602
Bud Neck 0.003935 0.000654 0.001904 0.001777 0.000820 0.001379 0.000759 0.001902 0.001157 0.001080 0.001055 0.001836
Bud Site 0.024771 0.003037 0.004472 0.005252 0.001912 0.003442 0.003511 0.008960 0.004385 0.003275 0.003760 0.007006
Cell Periphery 0.000229 0.000054 0.000162 0.000072 0.000034 0.000024 0.000039 0.000074 0.000055 0.000036 0.000071 0.000073
Cytoplasm 0.680500* 0.858841* 0.557958* 0.735631* 0.829566* 0.863642* 0.803905* 0.709260* 0.813435* 0.861661* 0.740200* 0.723158*
Cytoplasmic Foci 0.161420 0.015248 0.007783 0.006152 0.034611 0.021876 0.003843 0.009406 0.048333 0.019141 0.004864 0.007691
Eisosomes 0.000046 0.000004 0.000006 0.000003 0.000006 0.000004 0.000001 0.000004 0.000010 0.000004 0.000002 0.000003
Endoplasmic Reticulum 0.005912 0.000335 0.000614 0.000316 0.000378 0.000153 0.000071 0.000191 0.000977 0.000228 0.000212 0.000256
Endosome 0.004053 0.000593 0.000584 0.001336 0.000621 0.000663 0.000451 0.001600 0.000992 0.000634 0.000486 0.001461
Golgi 0.001744 0.000704 0.000212 0.000427 0.000525 0.000842 0.000198 0.005781 0.000657 0.000786 0.000202 0.002960
Lipid Particles 0.001901 0.000230 0.000730 0.000697 0.000321 0.000245 0.000205 0.000486 0.000492 0.000239 0.000341 0.000597
Mitochondria 0.002212 0.000537 0.000314 0.000335 0.000441 0.000354 0.000137 0.000467 0.000633 0.000430 0.000183 0.000397
Mitotic Spindle 0.002320 0.001225 0.002686 0.073908 0.000780 0.003221 0.006272 0.053964 0.000946 0.002398 0.005343 0.064475
None 0.001803 0.007320 0.007746 0.009103 0.006589 0.007175 0.009847 0.014678 0.006071 0.007235 0.009303 0.011740
Nuclear Periphery 0.000132 0.000080 0.000559 0.000337 0.000101 0.000086 0.000097 0.000238 0.000104 0.000084 0.000217 0.000291
Nuclear Periphery Foci 0.002031 0.002090 0.002171 0.001585 0.000618 0.000896 0.000934 0.001532 0.000771 0.001388 0.001255 0.001560
Nucleolus 0.000276 0.000148 0.000125 0.000410 0.000154 0.000361 0.000128 0.000194 0.000167 0.000273 0.000127 0.000308
Nucleus 0.062967 0.094082 0.381979* 0.139711* 0.101450 0.074979 0.158710* 0.165592* 0.097285 0.082861 0.216540* 0.151952*
Peroxisomes 0.001448 0.000151 0.000118 0.000270 0.000137 0.000555 0.000089 0.000323 0.000278 0.000389 0.000096 0.000295
Vacuole 0.039985 0.014074 0.029388 0.021726 0.020591 0.019698 0.010569 0.019545 0.022690 0.017377 0.015443 0.020694
Vacuole Periphery 0.001805 0.000466 0.000086 0.000738 0.000278 0.000140 0.000138 0.004770 0.000444 0.000274 0.000124 0.002645

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 5.76 -2.10 3.74 -5.20 5.80 -1.99 -2.95 -2.41 -1.09 -1.98 -1.69 -5.97 -3.04 -1.50 -0.94
Bud Neck 3.15 0.60 1.16 -10.73 1.75 -2.27 -2.18 -10.50 -5.76 -8.33 0.19 -3.12 -10.44 -9.36 -7.43
Bud Site 5.95 5.14 4.98 -5.91 -1.02 -2.75 -2.45 -7.70 -5.48 -4.55 1.58 0.20 -4.53 -7.76 -4.66
Cell Periphery 8.63 -1.40 6.40 -5.79 9.22 6.40 -4.69 -12.84 -14.90 -8.18 5.90 -8.49 -8.72 -13.93 1.43
Cytoplasm -12.65 11.79 4.65 35.13 -11.44 -5.74 13.41 33.63 35.71 21.76 -10.44 22.67 39.63 50.01 13.80
Cytoplasmic Foci 16.09 16.77 16.24 1.14 -3.28 6.52 19.51 9.48 2.91 -7.41 15.18 23.52 17.53 3.47 -8.50
Eisosomes 7.20 5.93 6.94 -3.30 7.51 3.63 11.52 -1.37 -4.95 -14.01 7.85 7.33 5.70 -5.82 -4.73
Endoplasmic Reticulum 4.07 3.58 4.18 2.73 9.56 4.45 5.97 3.74 -1.76 -6.17 4.65 4.05 4.90 1.39 4.68
Endosome 6.86 6.10 6.11 -1.48 0.64 -0.64 2.05 -2.58 -1.61 -3.52 3.31 4.15 0.60 -2.06 -2.84
Golgi 3.17 5.89 6.00 1.75 0.99 -0.68 2.15 -1.25 -0.69 -2.02 -0.30 3.00 -0.18 0.09 -2.05
Lipid Particles 5.64 0.93 -1.35 -12.51 -3.56 0.98 -4.56 -11.91 -11.76 -8.71 4.88 -5.64 -14.71 -16.97 -10.32
Mitochondria 1.28 1.38 1.49 0.55 5.02 0.52 1.85 0.80 0.37 -4.53 0.87 2.08 1.64 0.66 -3.98
Mitotic Spindle 1.27 -2.24 -17.96 -18.33 -17.62 -3.32 -5.78 -15.28 -14.39 -12.88 -3.02 -6.39 -23.79 -23.11 -20.98
None -6.85 -8.11 -9.96 -1.30 -2.01 -0.36 -3.63 -6.06 -5.28 -3.68 -1.60 -4.41 -6.58 -4.76 -3.08
Nuclear Periphery 2.25 -17.89 -19.35 -21.47 1.75 -0.64 -7.56 -19.66 -19.46 -15.92 0.21 -16.84 -27.88 -28.82 -14.31
Nuclear Periphery Foci -0.87 -4.04 -3.69 -2.98 0.27 -0.87 -4.30 -9.00 -4.94 -3.51 -3.19 -6.85 -12.02 -5.89 -3.63
Nucleolus 1.53 1.52 -2.36 -4.60 -5.34 -1.02 -1.07 -8.40 -0.91 -7.18 -0.89 -0.49 -8.89 -1.98 -8.20
Nucleus -5.18 -31.47 -12.68 -13.11 26.94 8.76 -19.50 -18.04 -23.79 -0.20 4.82 -32.21 -21.57 -26.76 15.79
Peroxisomes 4.69 4.81 4.14 -1.45 -1.84 -3.38 1.87 -3.20 2.22 -3.88 -1.41 4.85 0.11 1.39 -3.64
Vacuole 5.42 -5.12 -11.15 -22.13 -4.27 -2.56 -4.83 -19.46 -17.36 -16.13 0.58 -9.01 -27.97 -27.72 -18.23
Vacuole Periphery 2.66 3.54 3.28 0.93 -1.19 1.59 2.03 -1.26 -1.56 -1.65 1.47 3.60 -0.41 -1.14 -1.87
Standard name
Human Ortholog
Description Protein of unknown function; non-essential gene; interacts with the DNA helicase Hpr5p; YGL101W has a paralog, YBR242W, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ygk1

Ygk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ygk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available