ORF | |
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Human Ortholog | |
Description | Protein of unknown function, predicted to be palmitoylated; SWAT-GFP, seamless-GFP and mCherry C-terminal fusion proteins localize to the cytosol, while N-terminal GFP fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 29 | 61 | 39 | 15 | 20 | 20 | – | 24 | 12 | 17 | 15 | 20 | 28 | 41 | 24 | 21 | 16 | 11 | 2 | 6 | 2 | 4 | 13 | 9 |
Bud | 2 | 4 | 13 | 3 | 8 | 2 | – | 7 | 7 | 5 | 10 | 5 | 7 | 7 | 1 | 1 | 3 | 0 | 0 | 3 | 0 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 9 | 6 | 3 | 5 | 8 |
Bud Site | 1 | 0 | 1 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 91 | 241 | 113 | 94 | 80 | 126 | – | 128 | 90 | 95 | 110 | 135 | 168 | 200 | 217 | 203 | 130 | 93 | 109 | 297 | 156 | 169 | 286 | 276 |
Cytoplasm | 43 | 74 | 41 | 34 | 51 | 64 | – | 149 | 78 | 79 | 102 | 102 | 72 | 99 | 118 | 45 | 33 | 36 | 8 | 12 | 1 | 4 | 13 | 13 |
Endoplasmic Reticulum | 3 | 2 | 2 | 2 | 2 | 10 | – | 7 | 8 | 5 | 5 | 8 | 4 | 1 | 5 | 22 | 7 | 12 | 1 | 4 | 1 | 1 | 8 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 3 | 1 | 0 | 2 | 1 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 4 | 2 | 0 | 2 | 0 | 1 | 1 | 0 |
Mitochondria | 9 | 83 | 78 | 107 | 68 | 118 | – | 230 | 150 | 155 | 197 | 257 | 32 | 68 | 46 | 26 | 22 | 16 | 2 | 6 | 4 | 4 | 3 | 5 |
Nucleus | 0 | 1 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 2 | 0 | 2 | 1 | 4 | – | 2 | 3 | 1 | 1 | 0 | 11 | 17 | 23 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 2 | 2 | 4 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 0 | 0 | – | 9 | 1 | 2 | 4 | 6 | 1 | 2 | 2 | 6 | 3 | 2 | 0 | 4 | 1 | 1 | 2 | 2 |
Unique Cell Count | 119 | 303 | 164 | 147 | 151 | 214 | 370 | 223 | 224 | 287 | 358 | 218 | 275 | 295 | 248 | 164 | 132 | 137 | 355 | 179 | 193 | 344 | 336 | |
Labelled Cell Count | 178 | 471 | 288 | 261 | 231 | 344 | 558 | 349 | 360 | 448 | 539 | 326 | 441 | 440 | 336 | 220 | 179 | 137 | 355 | 179 | 193 | 344 | 336 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 7.2 | 5.2 | 5.9 | 6.4 | 5.3 | – | 5.4 | 5.1 | 5.0 | 4.9 | 4.9 | 7.5 | 7.6 | 7.5 | 9.8 | 9.5 | 9.2 | 7.7 | 8.0 | 8.2 |
Std Deviation (1e-4) | 0.7 | 0.8 | 1.1 | 1.5 | 1.5 | 0.8 | – | 1.1 | 1.4 | 1.0 | 1.2 | 1.2 | 1.6 | 1.9 | 1.9 | 2.0 | 1.8 | 1.9 | 1.4 | 2.1 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.19 | 0.31 | 0.03 | – | 0.06 | -0.04 | -0.05 | -0.08 | -0.09 | 0.53 | 0.55 | 0.53 | 0.91 | 0.86 | 0.82 | 0.57 | 0.62 | 0.66 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -3.2 | -2.4 | -3.8 | – | -5.7 | -5.3 | -4.5 | -5.8 | 0 | -2.8 | -2.3 | -4.7 | -4.3 | -3.4 | -3.5 |
Bud | -2.3 | -0.9 | -3.5 | – | -3.4 | -2.1 | -2.6 | -2.1 | 0 | -2.0 | -2.6 | -4.5 | -4.1 | -2.6 | -3.3 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.9 | -2.9 | -2.0 | – | -7.3 | -5.6 | -5.2 | -6.2 | 0 | 1.8 | 0.9 | 1.1 | 3.0 | 2.1 | 0.3 |
Cytoplasm | -0.4 | 1.7 | 1.1 | – | 3.4 | 2.1 | 2.2 | 2.3 | 0.8 | 1.7 | 2.4 | 3.2 | -1.7 | -1.1 | 0.4 |
Endoplasmic Reticulum | 0 | 0 | 1.9 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 3.2 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.5 | -0.4 | 1.5 | – | 3.2 | 3.9 | 4.3 | 4.4 | 0 | -7.0 | -4.9 | -7.4 | -8.5 | -6.7 | -6.5 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 3.7 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.601 | 2.5362 | 2.1989 | 1.9228 | 1.6144 | 2.1725 | 1.8945 | 2.3733 | 2.2214 | 1.9073 | 1.3124 | 2.1288 | 3.5218 | 4.0714 | 3.7965 | 3.5088 | 4.8812 | 3.5764 |
Actin | 0.0498 | 0.0015 | 0.0223 | 0.0003 | 0.0046 | 0.0021 | 0.05 | 0.0022 | 0.022 | 0.0372 | 0.0079 | 0.0079 | 0.0459 | 0.0011 | 0.0027 | 0.0132 | 0.0062 | 0.0157 |
Bud | 0.0011 | 0.0001 | 0.0006 | 0.0003 | 0.0006 | 0.0005 | 0.0045 | 0.0003 | 0.0048 | 0.0013 | 0.0007 | 0.0007 | 0.0023 | 0.0004 | 0.0009 | 0.0004 | 0.0021 | 0.0012 |
Bud Neck | 0.0084 | 0.005 | 0.0065 | 0.0572 | 0.0077 | 0.0132 | 0.0092 | 0.0038 | 0.0084 | 0.0124 | 0.0203 | 0.0149 | 0.0161 | 0.0033 | 0.0099 | 0.0171 | 0.0034 | 0.0134 |
Bud Periphery | 0.0063 | 0.0009 | 0.0042 | 0.0029 | 0.0066 | 0.0081 | 0.0242 | 0.0019 | 0.0081 | 0.0102 | 0.0043 | 0.0095 | 0.018 | 0.0025 | 0.0046 | 0.0026 | 0.0102 | 0.0085 |
Bud Site | 0.0237 | 0.0615 | 0.0298 | 0.0119 | 0.0083 | 0.0022 | 0.0212 | 0.1069 | 0.0808 | 0.0153 | 0.0359 | 0.0043 | 0.0281 | 0.1089 | 0.0571 | 0.0193 | 0.0048 | 0.0042 |
Cell Periphery | 0.5446 | 0.6683 | 0.6546 | 0.701 | 0.7087 | 0.7832 | 0.5048 | 0.5699 | 0.5664 | 0.6522 | 0.7193 | 0.6925 | 0.5877 | 0.552 | 0.6046 | 0.6052 | 0.6661 | 0.6617 |
Cytoplasm | 0.1696 | 0.241 | 0.2079 | 0.2147 | 0.1506 | 0.1703 | 0.2432 | 0.2987 | 0.2433 | 0.1744 | 0.137 | 0.2285 | 0.1493 | 0.3174 | 0.2544 | 0.2267 | 0.1229 | 0.2343 |
Cytoplasmic Foci | 0.0304 | 0.0008 | 0.0028 | 0.002 | 0.0061 | 0.0009 | 0.0123 | 0.0004 | 0.0079 | 0.0047 | 0.0005 | 0.0016 | 0.043 | 0.0004 | 0.0057 | 0.0096 | 0.0203 | 0.0019 |
Eisosomes | 0.0164 | 0.002 | 0.0048 | 0.0002 | 0.0004 | 0.0006 | 0.0034 | 0.0027 | 0.0053 | 0.0053 | 0.0011 | 0.0015 | 0.0025 | 0.0007 | 0.0023 | 0.0001 | 0.0008 | 0.0002 |
Endoplasmic Reticulum | 0.0036 | 0.0017 | 0.0047 | 0.0023 | 0.0057 | 0.0022 | 0.0138 | 0.0013 | 0.0027 | 0.0048 | 0.0014 | 0.0034 | 0.0037 | 0.001 | 0.002 | 0.0016 | 0.0021 | 0.0023 |
Endosome | 0.0152 | 0 | 0.0107 | 0.0005 | 0.0366 | 0.0001 | 0.0198 | 0 | 0.0115 | 0.0158 | 0.0001 | 0.0034 | 0.0184 | 0 | 0.0012 | 0.0008 | 0.072 | 0.0118 |
Golgi | 0.0086 | 0.0001 | 0.0178 | 0.0004 | 0.0025 | 0.0002 | 0.0057 | 0.0001 | 0.0047 | 0.0157 | 0.0003 | 0.0012 | 0.009 | 0.0001 | 0.0006 | 0.0027 | 0.039 | 0.0138 |
Lipid Particles | 0.0153 | 0.0004 | 0.0029 | 0.0004 | 0.0095 | 0.001 | 0.0074 | 0.0002 | 0.0038 | 0.0036 | 0.0006 | 0.0024 | 0.0212 | 0.0002 | 0.0009 | 0.0021 | 0.0235 | 0.0067 |
Mitochondria | 0.0149 | 0 | 0.0074 | 0.0001 | 0.001 | 0.0001 | 0.003 | 0 | 0.0013 | 0.0346 | 0 | 0.0004 | 0.0039 | 0 | 0.0001 | 0.0014 | 0.0059 | 0.0137 |
None | 0.0516 | 0.0152 | 0.0161 | 0.0018 | 0.0082 | 0.012 | 0.0275 | 0.0099 | 0.014 | 0.0042 | 0.0681 | 0.0162 | 0.0083 | 0.0099 | 0.0459 | 0.0004 | 0.009 | 0.0031 |
Nuclear Periphery | 0.0021 | 0.0001 | 0.0023 | 0.0001 | 0.0159 | 0.0001 | 0.0155 | 0.0001 | 0.001 | 0.0006 | 0.0001 | 0.0037 | 0.0069 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0004 |
Nucleolus | 0.0009 | 0.0001 | 0.0002 | 0.0012 | 0.0011 | 0.0002 | 0.0016 | 0.0001 | 0.0013 | 0.0003 | 0.0002 | 0.0004 | 0.0008 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0002 |
Nucleus | 0.001 | 0.0001 | 0.0005 | 0.0004 | 0.004 | 0.0002 | 0.0083 | 0.0002 | 0.0035 | 0.0004 | 0.0002 | 0.0008 | 0.0017 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0004 |
Peroxisomes | 0.024 | 0.0001 | 0.0008 | 0.0003 | 0.0009 | 0 | 0.008 | 0 | 0.003 | 0.0028 | 0 | 0.0003 | 0.0177 | 0 | 0.0014 | 0.094 | 0.0031 | 0.0012 |
Punctate Nuclear | 0.006 | 0 | 0.0003 | 0.0001 | 0.0108 | 0.0001 | 0.0075 | 0 | 0.001 | 0.0005 | 0.0001 | 0.002 | 0.0076 | 0 | 0.0015 | 0.0007 | 0.0004 | 0.0004 |
Vacuole | 0.0059 | 0.0009 | 0.0024 | 0.002 | 0.0091 | 0.0029 | 0.0077 | 0.0012 | 0.0047 | 0.0032 | 0.0018 | 0.0043 | 0.0067 | 0.0015 | 0.0035 | 0.0019 | 0.0058 | 0.0045 |
Vacuole Periphery | 0.0006 | 0 | 0.0004 | 0 | 0.0009 | 0 | 0.0013 | 0 | 0.0004 | 0.0007 | 0 | 0.0002 | 0.0008 | 0 | 0 | 0 | 0.0011 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.7383 | 6.9956 | 15.5731 | 15.5844 | 13.5282 | 23.4637 | 14.1464 | 15.9132 | 19.5204 | 13.8682 |
Translational Efficiency | 1.4611 | 2.1618 | 1.3101 | 1.4016 | 1.5663 | 0.7548 | 1.1623 | 1.9978 | 1.4121 | 1.3933 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1616 | 1432 | 82 | 474 | 581 | 1627 | 2082 | 1619 | 2197 | 3059 | 2164 | 2093 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 721.60 | 873.30 | 1162.03 | 999.90 | 652.69 | 856.09 | 984.80 | 982.42 | 703.38 | 864.15 | 991.52 | 986.38 |
Standard Deviation | 108.58 | 114.53 | 118.13 | 139.86 | 100.93 | 106.14 | 113.73 | 126.99 | 110.86 | 110.48 | 118.82 | 130.22 |
Intensity Change Log 2 | — | 0.275278 | 0.687376 | 0.470584 | — | 0.391365 | 0.593433 | 0.589942 | — | 0.331577 | 0.643521 | 0.528503 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003605 | 0.006356 | 0.036064 | 0.041195 | 0.004088 | 0.009748 | 0.020625 | 0.034738 | 0.003733 | 0.008160 | 0.021210 | 0.036200 |
Bud Neck | 0.060315 | 0.025557 | 0.021753 | 0.033418 | 0.045039 | 0.067670 | 0.049286 | 0.039712 | 0.056275 | 0.047956 | 0.048242 | 0.038287 |
Bud Site | 0.076369 | 0.081676 | 0.141166* | 0.192159* | 0.117197* | 0.054724 | 0.096383 | 0.158276* | 0.087166 | 0.067341 | 0.098080 | 0.165949* |
Cell Periphery | 0.229398* | 0.390646* | 0.377776* | 0.245512* | 0.438882* | 0.129115* | 0.209694* | 0.311952* | 0.284797* | 0.251545* | 0.216063* | 0.296905* |
Cytoplasm | 0.536743* | 0.453434* | 0.192864* | 0.294504* | 0.301391* | 0.614083* | 0.428908* | 0.335438* | 0.474504* | 0.538879* | 0.419964* | 0.326168* |
Cytoplasmic Foci | 0.025017 | 0.006659 | 0.030552 | 0.018769 | 0.014667 | 0.023835 | 0.011646 | 0.006779 | 0.022280 | 0.015795 | 0.012363 | 0.009495 |
Eisosomes | 0.007602 | 0.006990 | 0.019345 | 0.025338 | 0.004100 | 0.028793 | 0.043264 | 0.029695 | 0.006676 | 0.018587 | 0.042357 | 0.028708 |
Endoplasmic Reticulum | 0.004189 | 0.006119 | 0.051563 | 0.008947 | 0.013896 | 0.004175 | 0.017233 | 0.006428 | 0.006756 | 0.005085 | 0.018534 | 0.006998 |
Endosome | 0.007113 | 0.001412 | 0.004735 | 0.003063 | 0.004372 | 0.004313 | 0.000920 | 0.003914 | 0.006388 | 0.002955 | 0.001064 | 0.003722 |
Golgi | 0.005351 | 0.002953 | 0.007380 | 0.002742 | 0.002823 | 0.008571 | 0.001589 | 0.004485 | 0.004682 | 0.005941 | 0.001809 | 0.004090 |
Lipid Particles | 0.008131 | 0.001634 | 0.007796 | 0.002346 | 0.003677 | 0.006759 | 0.002960 | 0.001355 | 0.006953 | 0.004360 | 0.003143 | 0.001579 |
Mitochondria | 0.004617 | 0.003734 | 0.000270 | 0.000539 | 0.023289 | 0.004310 | 0.000570 | 0.003672 | 0.009555 | 0.004040 | 0.000559 | 0.002963 |
Mitotic Spindle | 0.000081 | 0.000918 | 0.000265 | 0.006496 | 0.000092 | 0.000139 | 0.000227 | 0.002443 | 0.000084 | 0.000504 | 0.000228 | 0.003361 |
None | 0.002824 | 0.000106 | 0.000043 | 0.000150 | 0.000714 | 0.000472 | 0.000539 | 0.000158 | 0.002266 | 0.000300 | 0.000521 | 0.000156 |
Nuclear Periphery | 0.000111 | 0.000086 | 0.000169 | 0.000136 | 0.000153 | 0.000075 | 0.000153 | 0.000102 | 0.000122 | 0.000080 | 0.000154 | 0.000110 |
Nuclear Periphery Foci | 0.000401 | 0.000223 | 0.004728 | 0.001173 | 0.001407 | 0.000270 | 0.001268 | 0.000590 | 0.000667 | 0.000248 | 0.001399 | 0.000722 |
Nucleolus | 0.000255 | 0.000052 | 0.000050 | 0.000095 | 0.000325 | 0.000110 | 0.000057 | 0.000038 | 0.000274 | 0.000082 | 0.000057 | 0.000051 |
Nucleus | 0.006215 | 0.001794 | 0.016320 | 0.016835 | 0.009043 | 0.010990 | 0.055977 | 0.015468 | 0.006963 | 0.006685 | 0.054474 | 0.015777 |
Peroxisomes | 0.004287 | 0.003656 | 0.001241 | 0.001153 | 0.003484 | 0.007138 | 0.001114 | 0.001303 | 0.004075 | 0.005508 | 0.001119 | 0.001269 |
Vacuole | 0.016362 | 0.005300 | 0.085733 | 0.103175 | 0.007835 | 0.024139 | 0.057513 | 0.041966 | 0.014107 | 0.015320 | 0.058583 | 0.055828 |
Vacuole Periphery | 0.001014 | 0.000696 | 0.000188 | 0.002257 | 0.003528 | 0.000571 | 0.000073 | 0.001490 | 0.001679 | 0.000629 | 0.000077 | 0.001663 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.11 | -4.43 | -11.13 | -10.20 | -0.56 | -4.71 | -11.78 | -15.91 | -14.08 | -7.38 | -8.47 | -16.87 | -21.39 | -18.12 | -8.34 |
Bud Neck | 11.08 | 9.08 | 6.83 | -1.98 | -2.37 | -4.90 | -0.97 | 1.34 | 8.78 | 3.78 | 2.98 | 2.86 | 6.91 | 4.50 | 4.31 |
Bud Site | -1.03 | -3.31 | -10.65 | -9.87 | -2.18 | 8.20 | 2.64 | -4.63 | -17.99 | -10.16 | 5.11 | -2.47 | -14.07 | -18.89 | -11.98 |
Cell Periphery | -14.65 | -4.00 | -0.51 | 9.68 | 3.61 | 19.15* | 14.28 | 7.52 | -19.83 | -11.22 | 3.76 | 8.15 | -0.90 | -4.92 | -9.48 |
Cytoplasm | 8.10 | 15.42 | 17.63 | 11.20 | -4.27 | -24.70 | -10.28 | -2.70 | 32.30 | 11.25 | -8.09 | 6.74 | 17.47 | 28.31 | 12.16 |
Cytoplasmic Foci | 9.75 | -1.02 | 1.88 | -5.22 | 1.87 | -2.90 | 1.36 | 3.29 | 9.78 | 4.77 | 3.97 | 6.22 | 7.84 | 4.91 | 2.62 |
Eisosomes | 0.91 | -4.35 | -8.45 | -8.72 | -1.65 | -19.91 | -30.84 | -18.52 | -0.42 | 7.48 | -15.20 | -28.85 | -18.51 | -7.42 | 8.42 |
Endoplasmic Reticulum | -1.99 | -4.40 | -4.64 | -2.18 | 3.96 | 4.73 | -1.49 | 3.67 | -2.42 | 8.37 | 2.03 | -8.91 | -0.27 | -2.50 | 9.03 |
Endosome | 8.19 | 2.23 | 3.94 | -1.97 | 1.58 | 0.10 | 5.95 | 0.50 | 0.48 | -3.74 | 6.14 | 10.28 | 3.30 | -1.10 | -4.10 |
Golgi | 4.05 | -1.74 | 2.43 | 0.27 | 3.14 | -9.09 | 3.59 | -1.57 | 3.68 | -2.91 | -2.70 | 9.21 | 0.75 | 2.17 | -2.80 |
Lipid Particles | 6.19 | 0.18 | 5.57 | -1.25 | 4.23 | -3.28 | 1.14 | 3.69 | 7.57 | 9.02 | 3.11 | 5.16 | 7.27 | 6.22 | 8.39 |
Mitochondria | 1.15 | 13.52 | 11.32 | 4.45 | -1.29 | 8.19 | 9.99 | 8.16 | 0.72 | -3.98 | 7.08 | 13.47 | 7.33 | 1.50 | -3.96 |
Mitotic Spindle | -1.40 | -6.22 | -2.02 | -1.72 | -1.97 | -2.18 | -12.80 | -2.65 | -2.60 | -2.50 | -1.50 | -15.97 | -3.30 | -2.76 | -3.16 |
None | 3.44 | 3.52 | 3.37 | -1.30 | -2.92 | 0.51 | 0.40 | 1.07 | 4.87 | 6.40 | 3.29 | 2.93 | 3.53 | 3.77 | 6.38 |
Nuclear Periphery | 1.55 | -1.76 | -0.79 | -1.94 | 0.92 | 3.35 | -0.02 | 2.08 | -2.81 | 5.08 | 3.12 | -2.23 | 0.82 | -3.14 | 4.40 |
Nuclear Periphery Foci | 2.39 | -6.11 | -4.58 | -6.23 | 4.92 | 1.03 | 0.14 | 0.73 | -3.16 | 5.75 | 1.41 | -2.36 | -0.20 | -5.59 | 6.26 |
Nucleolus | 4.61 | 4.82 | 2.37 | -0.71 | -0.76 | 1.40 | 1.67 | 1.76 | 7.96 | 6.93 | 3.40 | 3.86 | 3.88 | 2.10 | 0.48 |
Nucleus | 8.16 | -3.54 | -7.50 | -11.21 | -0.24 | -0.63 | -14.61 | -2.33 | -4.56 | 19.75 | 0.41 | -24.29 | -8.27 | -11.59 | 19.88 |
Peroxisomes | 1.49 | 10.10 | 10.58 | 7.10 | 0.28 | -5.39 | 5.60 | 5.18 | 10.35 | -0.88 | -3.63 | 10.99 | 11.13 | 12.11 | -0.83 |
Vacuole | 8.98 | -5.14 | -11.61 | -13.01 | -1.56 | -11.71 | -26.28 | -15.67 | -7.84 | 5.29 | -1.08 | -22.65 | -16.53 | -16.38 | 0.01 |
Vacuole Periphery | 2.06 | 14.50 | -0.97 | -1.23 | -1.66 | 9.21 | 10.90 | 3.06 | -1.54 | -2.39 | 8.74 | 16.82 | 0.07 | -1.89 | -2.95 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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