Standard name
Human Ortholog
Description Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 – 0 0 0 0 – 0 0 0 0 0 0 0 0.15 0.1 0.12 0 0 0 0 0 0
Cytoplasm 1.0 0.97 – 0.98 0.98 0.97 0.91 – 0.86 0.85 0.68 0.7 0.98 0.97 1.0 0.89 0.89 0.9 0.98 0.95 0.76 0.77 0.94 0.95
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0 0 0.06 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0 0.09 – 0.21 0.18 0.45 0.36 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0.08 0.06 0 0 0.08 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 – 1 0 0 0 – 1 2 0 2 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 1 – 1 3 1 1 – 1 4 3 5 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 11 – 1 5 4 8 – 2 8 4 6 3 7 8 22 15 17 0 0 0 0 0 0
Cytoplasm 116 229 – 173 297 250 406 – 193 227 172 258 82 182 241 135 131 128 114 226 38 19 167 225
Endoplasmic Reticulum 0 9 – 1 0 3 6 – 5 3 7 9 1 2 0 4 11 3 0 3 2 0 0 0
Endosome 0 0 – 0 0 1 2 – 0 0 1 2 0 0 0 0 1 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 6 5 3 0 0 0 0 0 0
Mitochondria 1 1 – 1 3 8 41 – 47 47 114 134 0 0 0 2 2 0 0 2 1 0 1 2
Nucleus 0 1 – 1 2 3 2 – 3 1 1 1 0 6 1 1 1 1 0 1 0 0 0 0
Nuclear Periphery 0 0 – 1 0 0 0 – 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 1 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 1 3 0 4 – 1 3 0 10 0 0 0 14 12 9 0 0 4 0 5 2
Unique Cell Count 116 236 177 304 258 447 225 267 252 370 84 188 242 151 148 142 116 238 51 26 179 237
Labelled Cell Count 117 253 181 313 270 471 253 295 303 428 86 197 250 184 178 162 116 238 51 26 179 237


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 8.8 – 7.7 7.7 6.3 6.3 – 5.2 5.6 5.0 5.2 7.7 8.1 8.1 12.3 12.4 11.2 – 7.5 7.8
Std Deviation (1e-4) 0.9 1.2 – 1.6 1.4 0.8 1.1 – 1.0 0.9 0.8 0.9 0.9 1.0 1.0 3.3 3.2 2.6 – 1.2 1.0
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3577 2.4557 1.976 1.6647 1.2973 1.8613 2.5961 3.8047 3.3238 3.0297 2.6147 3.2355 1.2042 2.5203 2.0433 2.065 1.4187 2.1642
Actin 0.0197 0.001 0.0166 0.0007 0.0036 0.0079 0.0144 0.0027 0.0052 0.0001 0.0127 0.0017 0.0093 0.0007 0.0059 0.0001 0.0004 0.0007
Bud 0.0007 0.0024 0.0003 0.0004 0.0348 0.0006 0.0013 0.0022 0.0008 0.0003 0.0018 0.0005 0.0009 0.0004 0.0005 0.0002 0.0002 0.0001
Bud Neck 0.0121 0.0003 0.0016 0.0007 0.0013 0.0013 0.0031 0.0002 0.0011 0.0001 0.0042 0.0011 0.0006 0.0033 0.0003 0.0002 0.0003 0.0008
Bud Periphery 0.001 0.0007 0.0003 0.0002 0.0162 0.0005 0.0018 0.001 0.0007 0.0003 0.002 0.0007 0.0009 0.0002 0.0003 0.0001 0.0001 0.0001
Bud Site 0.007 0.0044 0.004 0.0007 0.0046 0.0003 0.014 0.0105 0.0039 0.0007 0.0167 0.0004 0.0035 0.0042 0.0017 0.0001 0.0009 0.0001
Cell Periphery 0.0006 0.0005 0.0002 0.0001 0.0018 0.0001 0.0007 0.0003 0.0001 0.0001 0.0036 0.0001 0.0003 0.0002 0.0001 0 0 0
Cytoplasm 0.7031 0.9165 0.8515 0.8547 0.5864 0.8084 0.753 0.9176 0.8744 0.8674 0.7166 0.8779 0.8167 0.9083 0.8594 0.9401 0.8544 0.9575
Cytoplasmic Foci 0.0396 0.0132 0.0194 0.0247 0.0616 0.0464 0.0404 0.0063 0.0228 0.0143 0.0468 0.0212 0.0223 0.018 0.0228 0.0071 0.025 0.0103
Eisosomes 0.0003 0.0001 0.0002 0 0.0002 0.0001 0.0005 0 0 0 0.0026 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0094 0.0032 0.0045 0.0056 0.0023 0.003 0.0111 0.002 0.0057 0.0051 0.0049 0.0059 0.0089 0.0059 0.0046 0.0069 0.0032 0.0061
Endosome 0.0548 0.0089 0.0266 0.0864 0.0675 0.0316 0.0317 0.0062 0.0255 0.061 0.0942 0.0338 0.0357 0.0223 0.0314 0.0114 0.0198 0.011
Golgi 0.0135 0.0008 0.0043 0.0078 0.0273 0.0164 0.0067 0.0004 0.0058 0.0029 0.0194 0.0053 0.0116 0.0022 0.0053 0.0003 0.0022 0.0009
Lipid Particles 0.0262 0.0004 0.0033 0.0011 0.0115 0.0056 0.0098 0.0002 0.0004 0.0003 0.0106 0.0072 0.0041 0.0002 0.0011 0.0003 0.0062 0.0001
Mitochondria 0.0189 0.0007 0.0148 0.0017 0.0599 0.0263 0.0139 0.0002 0.0075 0.0032 0.0161 0.0088 0.0097 0.0004 0.0141 0.0001 0.0006 0.001
None 0.0348 0.042 0.0301 0.0033 0.066 0.0368 0.0637 0.0461 0.0293 0.0209 0.0086 0.0248 0.0599 0.0304 0.0405 0.0262 0.0249 0.0079
Nuclear Periphery 0.0032 0.0004 0.0012 0.0008 0.0016 0.0004 0.002 0.0005 0.0008 0.0021 0.0018 0.0011 0.0025 0.0007 0.0013 0.0013 0.0037 0.0007
Nucleolus 0.0069 0.0003 0.0002 0.0001 0.001 0 0.0006 0 0.0002 0 0.0006 0.0001 0.0002 0 0.0001 0 0.0022 0
Nucleus 0.0161 0.0009 0.001 0.0009 0.0033 0.0006 0.0012 0.0007 0.0014 0.0011 0.0022 0.0008 0.0016 0.0007 0.0008 0.001 0.0133 0.0007
Peroxisomes 0.0137 0.0001 0.0134 0.001 0.0277 0.0103 0.0216 0 0.0082 0.0001 0.0221 0.0043 0.0022 0.0001 0.0055 0 0.0026 0.0001
Punctate Nuclear 0.0092 0.0003 0.0008 0.0003 0.0014 0.001 0.0042 0.0002 0.0003 0.0002 0.0031 0.0007 0.0049 0.0002 0.0004 0.0002 0.0375 0.0001
Vacuole 0.0075 0.0025 0.0051 0.0072 0.0148 0.0018 0.0035 0.0023 0.0051 0.0152 0.0065 0.0026 0.003 0.0015 0.0032 0.0039 0.002 0.0013
Vacuole Periphery 0.0019 0.0004 0.0007 0.0018 0.0051 0.0007 0.0006 0.0003 0.0007 0.0045 0.0028 0.0009 0.0011 0.0003 0.0007 0.0005 0.0005 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.4754 6.8389 10.5398 14.0105 11.0634 9.9987 13.6966 14.8985 16.5845 13.203
Translational Efficiency 1.2363 1.8773 1.1961 1.0719 1.0799 1.4888 0.9515 0.7737 0.9401 1.1808

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1614 940 1586 1051 1517 1496 1771 95 3131 2436 3357 1146

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 648.16 839.44 1191.23 1000.56 849.25 859.41 1063.83 1045.31 745.59 851.70 1124.02 1004.27
Standard Deviation 76.97 138.16 160.25 141.80 99.69 112.24 124.81 133.73 134.05 123.28 156.19 141.68
Intensity Change Log 2 — 0.373077 0.878030 0.626386 — 0.017157 0.325006 0.299670 — 0.182089 0.590697 0.450245

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000227 0.000727 0.000325 0.000922 0.000222 0.000341 0.000107 0.000588 0.000225 0.000490 0.000210 0.000894
Bud Neck 0.013383 0.005151 0.000806 0.004019 0.003649 0.006473 0.002074 0.002864 0.008667 0.005963 0.001475 0.003923
Bud Site 0.024839 0.029464 0.007991 0.053759 0.009513 0.022131 0.008483 0.010881 0.017413 0.024960 0.008251 0.050205
Cell Periphery 0.000285 0.000116 0.000078 0.000105 0.000114 0.000123 0.000050 0.000141 0.000202 0.000120 0.000063 0.000108
Cytoplasm 0.512431* 0.556825* 0.866455* 0.621267* 0.599430* 0.567536* 0.733667* 0.381051* 0.554583* 0.563403* 0.796402* 0.601354*
Cytoplasmic Foci 0.293173* 0.235650* 0.025286 0.062644 0.246729* 0.246342* 0.043710 0.101078 0.270670* 0.242216* 0.035006 0.065830
Eisosomes 0.000274 0.000047 0.000005 0.000021 0.000061 0.000067 0.000013 0.000062 0.000170 0.000059 0.000010 0.000025
Endoplasmic Reticulum 0.005457 0.001423 0.000682 0.000778 0.003945 0.001752 0.000663 0.001207 0.004724 0.001625 0.000672 0.000813
Endosome 0.019710 0.034651 0.002044 0.012621 0.016509 0.027779 0.004719 0.029913 0.018159 0.030431 0.003455 0.014055
Golgi 0.020079 0.006950 0.000419 0.009014 0.009564 0.011065 0.000230 0.011721 0.014984 0.009477 0.000319 0.009239
Lipid Particles 0.008925 0.002489 0.000746 0.001195 0.003386 0.002569 0.000843 0.010983 0.006241 0.002538 0.000797 0.002007
Mitochondria 0.014912 0.002235 0.000077 0.004171 0.002575 0.002471 0.000155 0.020677 0.008935 0.002380 0.000118 0.005539
Mitotic Spindle 0.000245 0.001661 0.002690 0.033885 0.001824 0.002955 0.004097 0.002863 0.001010 0.002455 0.003432 0.031313
None 0.002885 0.001242 0.003185 0.001216 0.001852 0.000543 0.001475 0.001472 0.002384 0.000813 0.002283 0.001237
Nuclear Periphery 0.000360 0.000114 0.000157 0.000347 0.000076 0.000044 0.000284 0.000251 0.000223 0.000071 0.000224 0.000339
Nuclear Periphery Foci 0.000255 0.000914 0.004742 0.001288 0.000395 0.000314 0.001618 0.002178 0.000323 0.000546 0.003094 0.001362
Nucleolus 0.000505 0.000425 0.000091 0.000227 0.000236 0.000157 0.000209 0.000268 0.000375 0.000261 0.000153 0.000231
Nucleus 0.023017 0.010360 0.050968 0.018123 0.020097 0.007980 0.039591 0.050606 0.021602 0.008899 0.044966 0.020815
Peroxisomes 0.002575 0.003672 0.000111 0.002539 0.001608 0.002534 0.000474 0.001002 0.002106 0.002973 0.000303 0.002412
Vacuole 0.048048 0.100666 0.032919 0.168023 0.073812 0.092147 0.156642 0.368714* 0.060531 0.095434 0.098190 0.184660
Vacuole Periphery 0.008416 0.005219 0.000223 0.003835 0.004405 0.004676 0.000895 0.001479 0.006473 0.004886 0.000578 0.003639

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.64 -2.41 -2.18 0.94 -0.97 -1.22 1.80 -0.58 0.12 -1.30 -2.88 -0.28 -2.20 -0.04 -2.12
Bud Neck 9.83 16.81 13.39 4.70 -4.70 -4.43 4.54 3.44 6.59 -0.12 4.86 16.61 11.88 6.91 -3.14
Bud Site -1.78 9.23 -0.60 0.62 -5.62 -8.88 2.15 0.11 6.59 -1.28 -5.43 9.11 -2.66 -0.20 -5.97
Cell Periphery 11.85 15.25 15.59 4.50 2.42 -1.12 9.79 1.49 2.01 -2.22 9.65 17.99 13.56 6.20 -0.38
Cytoplasm -4.10 -45.35 -6.03 -1.72 25.20 3.36 -10.31 6.13 5.15 8.97 -1.28 -34.52 -0.99 -0.10 19.63
Cytoplasmic Foci 7.56 53.40 45.78 28.57 -7.39 0.11 40.37 15.00 14.80 -3.30 5.61 65.72 53.16 42.17 -5.67
Eisosomes 12.04 14.37 13.68 8.28 -8.55 -1.23 14.23 2.81 3.58 -5.59 10.84 16.17 14.98 12.47 -7.55
Endoplasmic Reticulum 5.94 7.21 7.64 4.40 2.32 2.80 5.10 4.60 2.12 -0.18 5.78 8.76 9.09 4.02 2.06
Endosome -6.57 19.92 11.38 12.75 -4.37 -7.28 13.07 0.43 2.80 -2.34 -9.57 23.08 10.69 16.84 -4.11
Golgi 13.28 24.66 11.77 2.59 -2.63 -1.54 18.32 1.15 1.47 -1.09 7.73 30.03 9.31 4.69 -2.93
Lipid Particles 9.86 13.09 12.98 5.66 0.22 2.67 12.24 -0.38 -0.59 -1.03 9.92 16.03 10.48 3.29 -1.04
Mitochondria 8.68 12.17 9.30 0.52 -2.11 0.11 3.89 -0.70 -0.71 -1.00 7.41 12.49 5.67 0.16 -2.17
Mitotic Spindle -1.84 -7.08 -5.77 -5.19 -4.77 -1.04 -2.49 -0.34 0.71 2.11 -2.07 -4.88 -5.52 -4.93 -4.46
None 2.30 0.16 3.61 0.19 6.21 2.69 1.16 0.84 -2.43 -0.27 3.91 1.06 3.54 -1.29 4.77
Nuclear Periphery 1.21 0.70 -0.17 -5.57 -3.81 4.43 -16.76 -6.35 -7.32 1.92 1.45 -0.73 -1.48 -7.51 -2.09
Nuclear Periphery Foci -5.80 -14.12 -9.55 -2.16 11.10 2.27 -11.66 -3.42 -3.59 -0.92 -4.47 -16.85 -9.82 -7.07 10.21
Nucleolus 0.13 3.65 2.80 1.18 -3.10 2.45 1.12 1.25 -0.66 0.56 0.91 3.74 3.00 0.97 -1.16
Nucleus 7.36 -16.25 -3.81 -10.99 12.20 13.58 -14.86 -1.76 -3.36 1.29 13.78 -21.52 -5.48 -13.99 10.65
Peroxisomes -2.14 15.72 3.51 4.07 -3.03 -2.85 4.26 3.15 9.14 -1.95 -3.25 11.31 2.61 4.62 -2.72
Vacuole -11.89 -0.56 -26.32 -18.30 -25.61 -4.63 -21.12 -10.74 -10.18 -7.01 -12.10 -18.55 -26.89 -22.05 -17.89
Vacuole Periphery 3.40 13.03 7.57 3.67 -1.97 -0.32 5.75 5.67 6.45 -0.10 2.66 13.12 6.75 4.55 -1.63
Standard name
Human Ortholog
Description Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination
Localization
Cell Percentages cytoplasm (93%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Cue3

Cue3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cue3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available