Standard name
Human Ortholog
Description Subunit of a heterodimeric guanine nucleotide exchange factor (GEF); subunit of the Mon1-Ccz1 GEF complex which stimulates nucleotide exchange and activation of Ypt7p, a Rab family GTPase involved in membrane tethering and fusion events at the late endosome and vacuole; GEF activity is stimulated by membrane association and anionic phospholipids; role in localizing Ypt7p to the vacuolar membrane; required for autophagy, the CVT pathway and mitophagy; potential Cdc28 substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.93 0.99 0.94 0.95 0.95 0.94 0.93 0.9 0.61 0.81 0.54 0.73 1.0 1.0 0.98 0.92 0.95 – 0.73 0.83 0.61 0.84 0.81 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.08 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.12 0 0 0 0 0.05 0.06 0.1 0.51 0.26 0.68 0.38 0 0 0 0.05 0 – 0.17 0.07 0.19 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – 0 3 1 0 0 0
Bud 0 0 2 0 0 0 1 4 1 3 2 4 0 0 0 1 1 – 0 1 2 0 1 3
Bud Neck 0 4 4 1 0 3 7 9 2 4 1 2 0 0 0 4 0 – 0 2 3 0 1 0
Bud Site 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 5 2 0 0 1 0 1 0 0 0 0 0 0 6 4 – 0 1 0 0 0 0
Cytoplasm 104 283 91 124 160 161 215 218 43 149 78 144 37 96 88 238 295 – 85 249 67 25 126 123
Endoplasmic Reticulum 0 0 1 0 0 0 0 1 1 0 1 0 0 0 0 29 24 – 1 0 2 0 0 1
Endosome 0 0 0 1 0 2 0 1 0 0 0 0 0 0 0 9 8 – 3 4 0 0 2 2
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 – 1 0 0 0 0 2
Mitochondria 13 2 0 4 2 9 15 23 36 49 98 75 0 0 1 12 4 – 19 20 20 1 6 5
Nucleus 0 0 3 0 1 1 1 1 3 0 0 0 0 0 0 1 0 – 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 2 3 4 3 6 2 7 5 12 0 0 0 8 3 – 1 5 1 1 3 2
Vac/Vac Membrane 0 1 0 2 5 1 0 4 0 1 0 1 0 1 0 3 9 – 0 3 2 0 5 1
Unique Cell Count 112 286 97 130 168 171 231 242 71 185 145 196 37 96 90 260 312 117 300 110 30 156 148
Labelled Cell Count 117 291 107 137 171 181 243 267 90 214 185 239 37 97 90 314 349 117 300 110 30 156 148


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 3.8 4.4 4.6 5.2 4.4 3.9 4.6 3.2 3.6 3.0 3.3 5.0 5.2 5.3 5.3 5.4 4.7 – 4.9 5.4
Std Deviation (1e-4) 0.4 0.6 0.8 2.2 3.0 2.4 1.6 2.0 0.9 1.4 0.7 0.7 0.8 1.4 2.0 1.7 1.5 1.3 – 1.5 2.0
Intensity Change (Log2) – – – 0.07 0.23 -0.0 -0.16 0.06 -0.45 -0.29 -0.55 -0.41 0.18 0.23 0.26 0.27 0.29 0.11 – 0.14 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4357 -0.5393 -0.8171 -1.0072 0.3803 -0.5282 1.1692 1.1022 0.4095 -0.0639 0.1362 0.2045 -0.4311 0.6775 -0.203 -0.4522 -0.9037 0.2625
Actin 0.0682 0.0345 0.0518 0.0002 0.0009 0.0011 0.1005 0.0398 0.0317 0.0001 0.0012 0.0031 0.0079 0.0375 0.008 0 0 0.0032
Bud 0.0038 0.0008 0.0006 0.0001 0.0001 0.0004 0.0009 0.0006 0.0013 0.0001 0.0001 0.0006 0.0002 0.0253 0.0043 0.0001 0 0.0002
Bud Neck 0.0077 0.003 0.0101 0.0002 0.0001 0.0014 0.003 0.0006 0.0008 0.0001 0.0002 0.001 0.0002 0.0004 0.0003 0.0002 0.0001 0.0005
Bud Periphery 0.0057 0.0005 0.0009 0.0001 0.0002 0.0004 0.0012 0.0007 0.002 0.0002 0.0001 0.002 0.0002 0.0038 0.0115 0.0001 0 0.0003
Bud Site 0.0157 0.0074 0.0062 0.0002 0.0002 0.0013 0.0063 0.003 0.0104 0.0002 0.0002 0.0005 0.0009 0.0175 0.007 0.0001 0 0.0003
Cell Periphery 0.0013 0.0005 0.0003 0 0.0001 0.0006 0.0007 0.0005 0.0004 0.0001 0.0001 0.0003 0.0001 0.0007 0.0004 0.0001 0 0.0001
Cytoplasm 0.1185 0.2165 0.1602 0.0853 0.0388 0.2764 0.1612 0.22 0.1527 0.1144 0.0678 0.1622 0.2017 0.2829 0.2499 0.2009 0.1497 0.357
Cytoplasmic Foci 0.1234 0.2062 0.1526 0.1422 0.0314 0.1881 0.0825 0.0936 0.1703 0.0695 0.2074 0.1781 0.209 0.0921 0.1561 0.2258 0.1546 0.2718
Eisosomes 0.0012 0.0002 0.0008 0 0.0002 0.0001 0.001 0.0003 0.0004 0.0001 0.0002 0.0002 0.0001 0.0002 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0075 0.0049 0.0051 0.0029 0.002 0.0107 0.0174 0.0097 0.0048 0.0019 0.0065 0.0047 0.0026 0.007 0.0086 0.0008 0.0024 0.0025
Endosome 0.198 0.1632 0.1473 0.3755 0.0151 0.1415 0.0792 0.1055 0.2092 0.1779 0.1503 0.1447 0.2634 0.1338 0.2402 0.2246 0.249 0.1425
Golgi 0.0099 0.0094 0.0086 0.0061 0.0084 0.0279 0.0104 0.0224 0.0109 0.0066 0.0037 0.0115 0.0102 0.006 0.0147 0.0031 0.0058 0.0409
Lipid Particles 0.0484 0.0145 0.0311 0.0587 0.0719 0.0617 0.0329 0.0152 0.0414 0.0013 0.0167 0.0238 0.0662 0.013 0.0121 0.013 0.003 0.0462
Mitochondria 0.0514 0.0033 0.0113 0.0038 0.3071 0.0072 0.0114 0.0148 0.0082 0.0021 0.0021 0.0596 0.0071 0.0224 0.019 0.0004 0.0008 0.0266
None 0.2137 0.3129 0.3779 0.256 0.3339 0.25 0.2446 0.4227 0.2509 0.6113 0.3553 0.3729 0.12 0.3241 0.2354 0.3046 0.4123 0.0804
Nuclear Periphery 0.0039 0.0027 0.0032 0.0029 0.0375 0.0014 0.0674 0.0111 0.004 0.0011 0.1183 0.0019 0.0014 0.0028 0.0038 0.0006 0.002 0.0014
Nucleolus 0.027 0.0005 0.0007 0.0003 0.0007 0.0006 0.0053 0.0005 0.0019 0.0004 0.0033 0.0008 0.0003 0.0004 0.0003 0.0004 0.0003 0.0002
Nucleus 0.0077 0.002 0.0013 0.0013 0.1337 0.001 0.0625 0.0029 0.0274 0.0011 0.0391 0.0015 0.0006 0.0019 0.0012 0.0025 0.0012 0.0011
Peroxisomes 0.008 0.0029 0.009 0.02 0.0087 0.0079 0.0121 0.0024 0.0287 0.0006 0.0024 0.0092 0.072 0.0016 0.0027 0.0009 0.0005 0.0068
Punctate Nuclear 0.0035 0.0039 0.0026 0.0018 0.0018 0.0015 0.0428 0.0019 0.0069 0.0006 0.0168 0.0031 0.0097 0.0031 0.0037 0.0012 0.001 0.0023
Vacuole 0.0645 0.0089 0.0154 0.0367 0.0034 0.0168 0.049 0.0216 0.0331 0.0086 0.0048 0.0112 0.024 0.0208 0.0167 0.0197 0.0151 0.0135
Vacuole Periphery 0.0111 0.0014 0.0028 0.0058 0.0039 0.002 0.0076 0.0101 0.0026 0.0017 0.0036 0.0072 0.0022 0.0029 0.0039 0.001 0.0021 0.0023

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.8428 8.6658 8.7 9.9131 7.0237 11.9683 11.2102 9.9143 10.3503 10.9746
Translational Efficiency 0.8101 0.7253 0.6581 0.4861 0.7048 0.5652 0.6615 0.6404 0.5774 0.5503

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
60 1210 124 345 241 1303 898 39 301 2513 1022 384

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 626.78 674.01 960.35 885.27 705.10 701.91 874.50 847.76 689.49 688.48 884.92 881.46
Standard Deviation 63.59 84.28 115.86 103.53 67.52 96.35 96.72 118.19 73.72 91.80 103.12 105.72
Intensity Change Log 2 — 0.104811 0.615601 0.498158 — -0.006542 0.310631 0.265828 — 0.046932 0.462198 0.379833

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000207 0.000524 0.002003 0.001277 0.000124 0.000340 0.000474 0.000201 0.000141 0.000428 0.000659 0.001168
Bud Neck 0.033963 0.043514 0.029257 0.022493 0.056048 0.033142 0.033827 0.015329 0.051645 0.038136 0.033273 0.021766
Bud Site 0.007377 0.021260 0.023854 0.080385 0.007105 0.020931 0.016362 0.013426 0.007159 0.021089 0.017271 0.073584
Cell Periphery 0.000191 0.000123 0.000170 0.000142 0.000109 0.000160 0.000067 0.000135 0.000125 0.000142 0.000080 0.000142
Cytoplasm 0.131232* 0.139317* 0.035453 0.233315* 0.155766* 0.115245* 0.151455* 0.249169* 0.150875* 0.126835* 0.137380* 0.234925*
Cytoplasmic Foci 0.315331* 0.328223* 0.087590 0.086294 0.325721* 0.265308* 0.126519 0.071736 0.323650* 0.295601* 0.121796 0.084816
Eisosomes 0.000285 0.000144 0.000171 0.000071 0.000185 0.000137 0.000073 0.000091 0.000205 0.000140 0.000085 0.000073
Endoplasmic Reticulum 0.012228 0.001387 0.010903 0.002862 0.000515 0.001151 0.002613 0.001432 0.002850 0.001265 0.003619 0.002717
Endosome 0.220617* 0.189689 0.224532* 0.060867 0.227519* 0.247508* 0.198727 0.147931 0.226143* 0.219668* 0.201858* 0.069709
Golgi 0.038714 0.027098 0.006346 0.007795 0.011021 0.035299 0.001944 0.000595 0.016541 0.031350 0.002479 0.007064
Lipid Particles 0.052325 0.014931 0.013782 0.004536 0.016011 0.014722 0.005832 0.006437 0.023250 0.014823 0.006796 0.004729
Mitochondria 0.051247 0.037187 0.001559 0.004143 0.010299 0.048262 0.001366 0.057786 0.018461 0.042929 0.001389 0.009591
Mitotic Spindle 0.000291 0.007550 0.058104 0.067457 0.001017 0.013224 0.053430 0.019134 0.000873 0.010492 0.053997 0.062549
None 0.000388 0.001504 0.002530 0.001774 0.000521 0.001573 0.001362 0.000861 0.000495 0.001540 0.001504 0.001682
Nuclear Periphery 0.000444 0.000375 0.003596 0.001315 0.000510 0.000399 0.002624 0.000720 0.000497 0.000388 0.002742 0.001254
Nuclear Periphery Foci 0.000251 0.000635 0.021017 0.004563 0.000509 0.000403 0.007327 0.003164 0.000458 0.000515 0.008988 0.004421
Nucleolus 0.001451 0.003106 0.040603 0.002248 0.003482 0.002181 0.012672 0.002301 0.003077 0.002626 0.016061 0.002254
Nucleus 0.020964 0.019284 0.145706* 0.050058 0.053252 0.013174 0.084160 0.062388 0.046816 0.016116 0.091627 0.051310
Peroxisomes 0.034277 0.033920 0.069733 0.018108 0.026593 0.023336 0.035893 0.007361 0.028125 0.028432 0.039999 0.017016
Vacuole 0.067249 0.118835 0.219215* 0.348846* 0.099987 0.144899 0.261844* 0.336791* 0.093461 0.132349 0.256672* 0.347621*
Vacuole Periphery 0.010968 0.011395 0.003873 0.001452 0.003704 0.018607 0.001429 0.003012 0.005152 0.015134 0.001726 0.001610

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.29 -5.20 -3.65 -2.38 3.25 -4.97 -4.62 -2.73 1.46 2.97 -5.96 -6.37 -4.82 -2.92 -0.62
Bud Neck -0.92 -0.23 1.34 6.47 3.31 2.80 2.37 5.46 5.81 6.43 1.94 2.16 4.75 6.96 5.89
Bud Site -5.08 -5.10 -5.09 -3.08 -2.33 -6.06 -5.13 -1.54 0.21 -0.29 -8.23 -6.32 -5.49 -3.05 -3.58
Cell Periphery 1.71 -0.06 2.17 1.16 3.20 -2.85 3.72 -0.34 1.84 -2.41 -1.17 3.36 1.30 2.67 -1.99
Cytoplasm -0.44 3.67 -3.10 -5.08 -11.40 3.01 -0.57 -1.82 -2.77 -1.69 2.00 0.12 -3.85 -6.78 -4.74
Cytoplasmic Foci -0.54 10.26 10.84 33.53 0.68 4.33 15.90 11.08 9.79 1.83 2.40 18.36 21.56 36.97 7.74
Eisosomes 1.83 1.24 2.78 6.38 7.22 3.22 7.64 3.33 1.09 -1.96 3.36 5.82 6.79 8.00 3.35
Endoplasmic Reticulum 1.25 -0.26 1.01 -3.89 5.46 -4.15 -13.09 -4.19 -1.96 4.25 0.91 -1.06 -0.25 -4.71 2.75
Endosome 1.17 2.40 7.28 21.70 6.76 -1.27 4.90 4.53 5.86 2.14 0.31 5.51 12.48 28.69 13.16
Golgi 1.14 3.47 3.61 11.62 1.05 -6.46 3.08 3.37 15.63 4.41 -4.28 4.56 4.16 15.76 -0.29
Lipid Particles 3.46 3.66 4.51 8.96 4.28 0.61 4.77 0.92 0.70 -0.84 2.89 5.80 6.46 9.67 2.94
Mitochondria 0.92 3.29 3.31 12.79 0.40 -10.26 4.22 -0.54 1.19 -0.95 -6.05 4.78 3.47 12.60 -0.92
Mitotic Spindle -6.45 -5.15 -6.16 -5.23 2.19 -5.93 -10.32 -1.41 -0.91 0.68 -7.94 -11.52 -6.22 -4.92 0.98
None -2.99 -2.01 -4.22 -0.75 0.67 -1.59 -6.32 -1.67 0.48 1.21 -2.70 -6.27 -4.15 -0.58 0.01
Nuclear Periphery 0.41 -6.59 -8.58 -10.74 4.04 0.83 -13.32 -3.02 -3.85 8.42 0.99 -15.02 -9.18 -11.20 6.81
Nuclear Periphery Foci -3.15 -6.45 -5.38 -4.91 5.18 1.02 -9.11 -2.56 -2.60 1.30 -0.78 -10.96 -5.69 -5.61 5.20
Nucleolus -2.06 -5.21 -1.34 0.93 5.07 1.38 -7.40 0.32 -0.44 6.51 0.42 -9.07 0.67 0.37 9.53
Nucleus 0.20 -7.08 -5.96 -7.76 4.27 5.63 -4.75 -1.39 -3.47 0.56 5.19 -7.38 -3.68 -8.87 2.64
Peroxisomes 0.01 -0.98 3.74 10.42 4.18 0.95 0.29 5.31 7.27 6.62 -0.03 0.08 6.07 11.36 7.63
Vacuole -2.87 -6.75 -17.43 -20.16 -8.28 -4.58 -17.25 -5.68 -4.89 -1.80 -4.68 -18.83 -20.97 -20.23 -9.09
Vacuole Periphery -0.11 2.16 2.78 11.69 5.60 -13.87 5.06 2.25 13.06 -0.21 -9.29 4.43 5.07 20.97 3.15
Standard name
Human Ortholog
Description Subunit of a heterodimeric guanine nucleotide exchange factor (GEF); subunit of the Mon1-Ccz1 GEF complex which stimulates nucleotide exchange and activation of Ypt7p, a Rab family GTPase involved in membrane tethering and fusion events at the late endosome and vacuole; GEF activity is stimulated by membrane association and anionic phospholipids; role in localizing Ypt7p to the vacuolar membrane; required for autophagy, the CVT pathway and mitophagy; potential Cdc28 substrate
Localization
Cell Percentages mitochondrion (16%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mon1

Mon1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mon1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available