Standard name
Human Ortholog
Description Major isozyme of methylenetetrahydrofolate reductase; catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.36 0.5 0.34 0.47 0.29 0.56 0.35 0.46 0.42 0.45 0.45 0.35 – – – 0.58 0.56 0.51 0.16 0.24 0.16 0.32 0.41 0.47
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0.21 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.09 0 0.24 0.09 – – – 0 0 0 0 0 0 0.08 0 0
Nucleus 0.36 0.23 0.32 0.31 0.16 0.24 0.24 0.25 0.2 0.25 0.14 0.21 – – – 0.09 0.07 0.08 0.68 0.58 0.52 0.11 0.43 0.14
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.56 0.59 0.58 0.45 0.69 0.32 0.52 0.41 0.4 0.43 0.26 0.45 – – – 0.56 0.59 0.64 0.05 0.1 0.23 0 0.09 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 2 1 0 1 0 – – – 1 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 1 0 1 2 1 – – – 0 0 0 4 10 3 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 2 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 1 0 1 0 – – – 4 0 5 0 0 0 0 0 0
Cytoplasm 97 135 47 74 53 112 95 163 98 106 75 68 – – – 155 137 174 46 67 25 3 6 11
Endoplasmic Reticulum 0 4 0 0 0 2 0 4 1 0 1 0 – – – 5 10 12 1 0 1 0 0 0
Endosome 0 0 0 0 3 0 6 2 1 0 0 0 – – – 1 0 1 8 4 2 2 0 0
Golgi 0 0 0 1 1 1 1 1 0 1 3 0 – – – 1 4 3 1 0 1 0 0 0
Mitochondria 3 1 0 0 0 2 4 5 21 7 40 18 – – – 0 1 1 1 0 0 0 0 0
Nucleus 97 63 45 49 30 48 66 90 47 58 23 41 – – – 24 16 27 197 163 80 1 7 3
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 1 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 2 2 – – – 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 – – – 0 0 0 5 2 0 0 0 0
Vac/Vac Membrane 151 158 80 71 125 63 143 144 92 101 43 88 – – – 150 143 217 14 27 35 0 1 1
Unique Cell Count 268 269 139 159 182 200 273 355 232 236 165 197 269 243 340 290 282 155 12 17 24
Labelled Cell Count 348 362 172 195 212 228 316 413 263 274 191 219 341 311 440 290 282 155 12 17 24


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 8.9 9.7 10.2 11.5 10.2 10.1 10.6 9.3 9.8 8.5 9.5 – – – 12.0 12.3 12.1 – – –
Std Deviation (1e-4) 1.2 1.5 1.6 1.8 1.9 2.1 1.8 2.0 1.9 1.9 2.1 2.3 – – – 1.8 1.6 1.8 – – –
Intensity Change (Log2) – – – 0.07 0.24 0.06 0.05 0.12 -0.07 0.01 -0.2 -0.04 – – – 0.3 0.34 0.31 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cytoplasm 2.2 -0.9 4.0 0.2 2.4 1.6 2.1 2.1 0.1 – – – 4.6 4.2 3.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 0 3.7 0 6.2 3.7 – – – 0 0 0
Nucleus -0.3 -3.3 -1.7 -1.8 -1.6 -2.6 -1.6 -3.8 -2.4 – – – -6.0 -6.6 -6.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Vacuole -2.2 2.1 -4.8 -1.0 -3.4 -3.3 -2.8 -5.6 -2.3 – – – -0.3 0.2 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.2419 13.2468 11.6891 11.7965 9.4547 11.712 8.432 10.3811 9.4854 9.1676 8.8293 10.5317 7.7876 11.1656 10.3706 9.4834 7.7841 10.4671
Actin 0.0071 0 0.0033 0.0003 0.0002 0.001 0.0064 0.0001 0.0085 0.0312 0.0011 0.0011 0.1066 0.0039 0.0162 0.0078 0.1914 0.0152
Bud 0.0016 0 0.0004 0.0015 0.0001 0.0002 0.0008 0.0001 0.0002 0.0009 0.0003 0.0002 0.0034 0.0002 0.0002 0.0001 0.0013 0.0002
Bud Neck 0.0022 0.0002 0.0005 0.0006 0.0004 0.001 0.0005 0.0002 0.0003 0.0007 0.0012 0.001 0.008 0.0003 0.0002 0.0001 0.0012 0.0013
Bud Periphery 0.0012 0 0.0004 0.0023 0.0001 0.0002 0.001 0 0.0002 0.0022 0.0002 0.0002 0.0055 0.0001 0.0002 0 0.0018 0.0001
Bud Site 0.0029 0.0001 0.0017 0.001 0.0005 0.0002 0.0075 0.0003 0.0009 0.0009 0.0006 0.0002 0.0269 0.0004 0.0004 0.0001 0.0052 0.0001
Cell Periphery 0.0004 0 0.0002 0.0003 0 0.0001 0.0004 0 0.0001 0.0003 0.0001 0.0001 0.0011 0 0.0001 0 0.0002 0
Cytoplasm 0.0487 0.0433 0.0235 0.0305 0.0205 0.0248 0.0243 0.0696 0.0461 0.0497 0.0682 0.0331 0.0284 0.0822 0.0585 0.0798 0.0271 0.0574
Cytoplasmic Foci 0.0143 0.0001 0.0061 0.0006 0.0022 0.002 0.0287 0.0008 0.0055 0.0154 0.0144 0.0015 0.0353 0.0007 0.0103 0.0206 0.0264 0.0014
Eisosomes 0.0001 0 0.0002 0 0 0.0005 0.0001 0 0 0.0001 0 0 0.0014 0 0.0001 0 0.0008 0.0001
Endoplasmic Reticulum 0.0086 0.0005 0.001 0.0011 0.0018 0.0027 0.0052 0.0008 0.0038 0.0044 0.0052 0.0051 0.0076 0.0007 0.001 0.0002 0.003 0.0008
Endosome 0.0413 0.0003 0.0151 0.0049 0.0177 0.0131 0.0393 0.0021 0.007 0.0628 0.054 0.0092 0.0355 0.0012 0.0095 0.003 0.0168 0.0045
Golgi 0.0079 0 0.0022 0.0003 0.0007 0.0047 0.0041 0 0.003 0.0142 0.0063 0.0013 0.0125 0.0005 0.0023 0.0029 0.0266 0.0041
Lipid Particles 0.0101 0 0.0009 0.0002 0.0005 0.0046 0.0068 0 0.0048 0.0137 0.0028 0.0011 0.017 0.0004 0.0043 0.0224 0.0171 0.005
Mitochondria 0.0111 0.0001 0.0139 0.0056 0.001 0.0075 0.0113 0.0004 0.0023 0.0105 0.0052 0.0114 0.0086 0.0007 0.001 0.0003 0.0072 0.0009
None 0.0037 0.0001 0.0028 0.0005 0.0012 0.0037 0.0087 0.0004 0.003 0.0007 0.001 0.0009 0.0117 0.0004 0.0011 0.0005 0.0025 0.0004
Nuclear Periphery 0.0485 0.0162 0.0074 0.0093 0.0324 0.0166 0.0386 0.0146 0.017 0.012 0.0191 0.0106 0.0248 0.0141 0.0126 0.0079 0.0168 0.0116
Nucleolus 0.0087 0.0007 0.0012 0.0017 0.0054 0.0016 0.0014 0.0014 0.0007 0.0006 0.001 0.0009 0.0063 0.0014 0.0009 0.0002 0.0005 0.0008
Nucleus 0.7517 0.9357 0.8968 0.9112 0.893 0.9082 0.7875 0.905 0.8893 0.7517 0.8021 0.9115 0.6088 0.8864 0.8601 0.8121 0.6324 0.8924
Peroxisomes 0.0032 0 0.0069 0.0001 0.0001 0.0007 0.0048 0 0.0005 0.0085 0.0008 0.0004 0.0151 0.0002 0.0059 0.0285 0.0162 0.0013
Punctate Nuclear 0.0158 0.0008 0.0027 0.006 0.011 0.0023 0.0182 0.002 0.0046 0.0032 0.0022 0.0053 0.0198 0.0052 0.0092 0.0131 0.0039 0.0017
Vacuole 0.0071 0.0015 0.0093 0.0164 0.0087 0.0027 0.0023 0.0017 0.0014 0.0133 0.0096 0.004 0.0115 0.0006 0.0045 0.0002 0.0006 0.0005
Vacuole Periphery 0.0036 0.0003 0.0036 0.0056 0.0025 0.0017 0.002 0.0003 0.0008 0.0031 0.0045 0.0008 0.0043 0.0002 0.0016 0.0001 0.0009 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.5014 19.6947 15.0203 20.9204 17.8529 18.3493 18.2538 20.1602 23.3855 18.6432
Translational Efficiency 0.5501 0.4046 0.5072 0.6622 0.4411 0.6158 0.5154 0.6098 0.5373 0.4107

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1491 1462 2422 1538 2264 1363 2489 68 3755 2825 4911 1606

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 748.60 901.36 902.08 858.14 952.60 1023.28 881.95 890.06 871.60 960.18 891.88 859.49
Standard Deviation 100.80 172.89 108.80 108.85 130.47 155.54 99.32 93.53 155.76 175.65 104.59 108.43
Intensity Change Log 2 — 0.267908 0.269060 0.197018 — 0.103259 -0.111174 -0.097968 — 0.178036 0.068587 0.039317

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000060 0.000411 0.000180 0.002956 0.000109 0.000308 0.000110 0.000527 0.000090 0.000361 0.000144 0.002854
Bud Neck 0.002424 0.003890 0.007660 0.016457 0.003874 0.004209 0.004491 0.002208 0.003298 0.004044 0.006054 0.015854
Bud Site 0.000810 0.001992 0.002361 0.041468 0.001046 0.001949 0.002569 0.022422 0.000952 0.001971 0.002466 0.040661
Cell Periphery 0.000106 0.000148 0.000074 0.000295 0.000083 0.000077 0.000101 0.000129 0.000092 0.000114 0.000088 0.000288
Cytoplasm 0.242903* 0.206119* 0.094799 0.246727* 0.169912* 0.096607 0.254393* 0.393062* 0.198895* 0.153282* 0.175684* 0.252923*
Cytoplasmic Foci 0.009506 0.002325 0.000487 0.010174 0.003389 0.002171 0.002811 0.003778 0.005818 0.002250 0.001665 0.009903
Eisosomes 0.000013 0.000012 0.000011 0.000040 0.000009 0.000010 0.000018 0.000038 0.000010 0.000011 0.000014 0.000040
Endoplasmic Reticulum 0.000839 0.003635 0.003025 0.003533 0.002056 0.002759 0.001784 0.003006 0.001573 0.003212 0.002396 0.003511
Endosome 0.001407 0.000798 0.000366 0.008057 0.000568 0.001437 0.001277 0.001193 0.000901 0.001106 0.000827 0.007767
Golgi 0.000614 0.000371 0.000028 0.005947 0.000147 0.000440 0.000049 0.000063 0.000332 0.000404 0.000039 0.005698
Lipid Particles 0.001053 0.000609 0.000192 0.002455 0.000221 0.000643 0.000403 0.000664 0.000551 0.000625 0.000299 0.002380
Mitochondria 0.000568 0.000856 0.000974 0.006149 0.000497 0.001741 0.001058 0.000560 0.000525 0.001283 0.001017 0.005913
Mitotic Spindle 0.000325 0.003215 0.002255 0.076631 0.002257 0.009755 0.008676 0.001366 0.001490 0.006370 0.005510 0.073444
None 0.020678 0.016638 0.003892 0.009583 0.004952 0.006552 0.008409 0.027597 0.011196 0.011772 0.006181 0.010345
Nuclear Periphery 0.000392 0.000732 0.001789 0.002494 0.001157 0.001156 0.001899 0.001331 0.000853 0.000937 0.001845 0.002444
Nuclear Periphery Foci 0.000328 0.001169 0.000207 0.001314 0.000414 0.000521 0.000249 0.000438 0.000380 0.000856 0.000228 0.001277
Nucleolus 0.002068 0.001174 0.000287 0.001010 0.001216 0.001230 0.000562 0.000450 0.001554 0.001201 0.000426 0.000986
Nucleus 0.711797* 0.747890* 0.874440* 0.510824* 0.803150* 0.859475* 0.690739* 0.495771* 0.766876* 0.801727* 0.781337* 0.510187*
Peroxisomes 0.000351 0.000288 0.000036 0.002516 0.000169 0.000406 0.000376 0.000137 0.000241 0.000345 0.000208 0.002415
Vacuole 0.003587 0.007477 0.006871 0.047670 0.004644 0.008057 0.019308 0.045155 0.004224 0.007757 0.013174 0.047563
Vacuole Periphery 0.000173 0.000253 0.000068 0.003701 0.000132 0.000496 0.000717 0.000103 0.000148 0.000370 0.000397 0.003548

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -10.96 -12.34 -3.98 -2.53 -3.28 -3.81 -3.35 -4.94 -3.29 -4.65 -8.69 -10.20 -3.98 -2.77 -3.55
Bud Neck -3.30 -7.33 -12.11 -9.44 -4.25 -0.64 -2.01 -1.36 -0.94 -0.48 -2.13 -6.68 -11.24 -9.79 -6.94
Bud Site -3.73 -3.98 -9.12 -8.20 -7.97 -1.82 -4.08 -1.50 -1.41 -1.39 -3.69 -5.44 -9.15 -8.42 -8.30
Cell Periphery -3.67 3.13 -3.63 -1.60 -4.84 1.24 -0.31 -3.29 -3.81 -3.20 -2.95 1.81 -4.36 -3.31 -4.83
Cytoplasm 4.89 22.91 7.43 1.91 -17.06 11.29 -6.05 -1.01 -3.52 0.33 8.84 10.67 2.45 -5.30 -6.14
Cytoplasmic Foci 6.58 8.58 4.28 -3.82 -8.09 2.54 1.98 0.49 -0.94 -0.48 6.70 8.54 1.95 -4.43 -6.15
Eisosomes -0.82 -2.66 -19.00 -18.37 -18.02 -3.00 -16.28 -7.21 -6.95 -5.82 -3.18 -14.27 -22.30 -20.44 -16.38
Endoplasmic Reticulum -11.90 -17.92 -18.74 -4.24 -4.23 -3.94 -3.92 -4.28 -3.64 -3.79 -10.64 -14.24 -16.12 -7.09 -7.86
Endosome 1.27 2.05 -2.14 -4.30 -5.32 -1.71 -2.16 -3.75 -1.52 -2.54 -0.45 0.78 -4.04 -3.65 -4.70
Golgi 0.62 1.52 -0.99 -1.45 -1.97 -1.63 1.18 0.08 1.93 -4.37 -0.23 1.82 -1.48 -1.44 -1.96
Lipid Particles 0.88 1.77 -0.94 -2.50 -4.32 -1.96 -7.61 -5.60 -2.82 -4.30 -0.26 0.93 -2.96 -2.87 -4.05
Mitochondria -1.32 -5.13 -7.34 -5.86 -5.76 -2.02 -4.31 -1.61 1.72 2.84 -2.39 -6.75 -7.60 -4.08 -5.83
Mitotic Spindle -3.59 -4.20 -9.84 -8.74 -8.80 -3.00 -4.37 -1.61 0.71 0.77 -3.85 -5.32 -9.48 -7.87 -8.05
None 2.27 9.54 6.98 5.19 -7.32 -1.52 -2.99 -1.66 -1.26 -1.07 0.10 7.25 3.51 3.19 -4.34
Nuclear Periphery -6.84 -25.66 -22.60 -19.84 -12.19 0.04 -9.14 -5.04 -4.51 0.05 -0.99 -16.86 -19.51 -17.95 -11.23
Nuclear Periphery Foci -5.81 0.16 -4.70 0.43 -4.91 -0.17 2.53 -3.54 -2.94 -4.22 -3.80 2.20 -4.43 -1.32 -5.23
Nucleolus 1.58 3.28 2.36 1.16 -6.50 0.21 2.03 1.91 1.64 -0.23 1.18 3.82 2.58 1.54 -4.88
Nucleus -4.06 -20.16 11.38 15.28 33.06 -7.03 11.58 7.69 9.19 5.35 -5.83 -5.09 20.32 23.78 24.59
Peroxisomes 0.56 3.15 -1.41 -1.68 -2.68 -1.72 -0.31 0.33 1.96 0.47 -0.89 1.43 -1.89 -1.54 -2.30
Vacuole -4.82 -8.08 -23.46 -22.00 -21.63 -3.57 -16.32 -6.90 -6.72 -5.80 -5.57 -17.75 -24.19 -22.88 -19.63
Vacuole Periphery -0.79 2.68 -1.94 -1.67 -2.19 -2.65 -1.30 -0.84 2.46 1.18 -2.53 -0.87 -2.01 -1.43 -1.71
Standard name
Human Ortholog
Description Major isozyme of methylenetetrahydrofolate reductase; catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway
Localization
Cell Percentages cytoplasm (25%), nucleus (11%), mixed (63%)
Cell Cycle Regulation Yes
nucleus - T/G1 (0.00044)
Subcompartmental Group N/A

Met13

Met13


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Met13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available