Standard name | |
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Human Ortholog | |
Description | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 7 | 10 | 9 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 2 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 0 | 1 | 3 | 4 | – | 8 | 15 | 31 | 36 | 29 | 0 | 2 | 3 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 4 | 4 | 13 | 12 | 22 | – | 23 | 27 | 35 | 20 | 28 | 19 | 70 | 99 | 12 | 10 | 0 | 0 | 0 | 1 | 0 | 2 | 2 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 1 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 0 | 0 | 0 | 0 | 3 | 1 | – | 1 | 0 | 0 | 1 | 1 | 1 | 5 | 11 | 1 | 2 | 0 | 1 | 0 | 3 | 0 | 3 | 3 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 1 |
Mitochondria | 7 | 4 | 29 | 69 | 49 | 172 | – | 253 | 226 | 228 | 330 | 330 | 16 | 3 | 3 | 7 | 4 | 3 | 0 | 0 | 1 | 0 | 2 | 2 |
Nucleus | 256 | 94 | 104 | 220 | 428 | 336 | – | 514 | 322 | 413 | 396 | 453 | 451 | 551 | 585 | 118 | 60 | 14 | 246 | 105 | 102 | 106 | 300 | 442 |
Nuclear Periphery | 0 | 0 | 0 | 8 | 2 | 7 | – | 9 | 21 | 12 | 18 | 15 | 2 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 42 | 13 | 24 | 58 | 76 | 116 | – | 217 | 167 | 209 | 209 | 215 | 99 | 103 | 142 | 14 | 5 | 2 | 28 | 7 | 10 | 17 | 70 | 83 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 1 | 2 | 0 | – | 1 | 4 | 5 | 2 | 3 | 0 | 2 | 7 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 4 | 7 |
Vac/Vac Membrane | 1 | 3 | 0 | 1 | 9 | 5 | – | 5 | 6 | 4 | 10 | 5 | 3 | 3 | 8 | 7 | 6 | 2 | 4 | 3 | 2 | 6 | 19 | 33 |
Unique Cell Count | 278 | 105 | 120 | 266 | 495 | 474 | 764 | 512 | 656 | 680 | 710 | 500 | 658 | 748 | 145 | 79 | 18 | 286 | 126 | 127 | 136 | 412 | 593 | |
Labelled Cell Count | 309 | 120 | 162 | 372 | 585 | 665 | 1034 | 795 | 948 | 1034 | 1086 | 591 | 741 | 870 | 164 | 92 | 21 | 286 | 126 | 127 | 136 | 412 | 593 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.8 | 10.1 | 6.2 | 5.9 | 6.6 | 5.3 | – | 5.2 | 4.9 | 5.1 | 5.0 | 5.0 | 8.4 | 9.3 | 9.2 | 10.5 | 10.5 | 11.8 | 7.0 | 8.2 | 8.9 |
Std Deviation (1e-4) | 1.5 | 1.8 | 1.3 | 1.2 | 1.4 | 1.4 | – | 1.1 | 1.6 | 1.7 | 1.6 | 1.3 | 1.7 | 2.5 | 2.5 | 2.9 | 2.9 | 3.3 | 1.5 | 1.8 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.08 | -0.22 | – | -0.25 | -0.36 | -0.27 | -0.32 | -0.32 | 0.44 | 0.59 | 0.57 | 0.76 | 0.75 | 0.93 | 0.18 | 0.4 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7 | 0 | 0.6 | – | 0 | 0.9 | 0.9 | 0 | 0 | 0 | 2.5 | 3.1 | 1.7 | 2.5 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.4 | -4.2 | 2.5 | – | 2.0 | 0 | 2.3 | 4.9 | 4.6 | -7.9 | 0 | 0 | -4.6 | -3.5 | 0 |
Nucleus | -1.0 | -0.1 | -3.5 | – | -4.3 | -5.0 | -5.1 | -5.9 | -4.9 | 1.1 | -0.8 | -2.1 | -1.2 | -1.9 | -1.0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.4 | -1.2 | 1.0 | – | 1.9 | 0 | 2.6 | 2.4 | 2.3 | 0 | -1.2 | -0.3 | -2.4 | -2.7 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.0127 | 5.5648 | 4.2789 | 4.3786 | 4.1272 | 4.2886 | 3.5596 | 4.6418 | 4.2431 | 3.8389 | 3.1045 | 3.9609 | 5.8964 | 6.8661 | 6.6359 | 5.6853 | 5.619 | 5.955 |
Actin | 0.0276 | 0.0001 | 0.0157 | 0.0435 | 0.051 | 0.0011 | 0.0193 | 0.0089 | 0.0062 | 0 | 0.0168 | 0.0004 | 0.0166 | 0 | 0.011 | 0.0254 | 0 | 0.0005 |
Bud | 0.0007 | 0 | 0.0012 | 0.0012 | 0.0009 | 0.0004 | 0.0002 | 0 | 0.0003 | 0 | 0.0047 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0036 | 0.0001 | 0.0011 | 0.0024 | 0.0024 | 0.0022 | 0.0006 | 0.0003 | 0.002 | 0.0001 | 0.0018 | 0.0014 | 0.002 | 0.0001 | 0.0003 | 0.0005 | 0.0006 | 0.0022 |
Bud Periphery | 0.001 | 0 | 0.0017 | 0.001 | 0.0018 | 0.0014 | 0.0003 | 0 | 0.0006 | 0 | 0.0078 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Bud Site | 0.0107 | 0.0002 | 0.0117 | 0.0116 | 0.0026 | 0.0007 | 0.0005 | 0.0006 | 0.0012 | 0 | 0.0147 | 0.0002 | 0.0025 | 0.0001 | 0.0007 | 0.0005 | 0.0002 | 0.0003 |
Cell Periphery | 0.0003 | 0 | 0.0004 | 0.0002 | 0.0005 | 0.0004 | 0.0001 | 0 | 0.0005 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0076 | 0.0002 | 0.0033 | 0.0084 | 0.0013 | 0.0003 | 0.0006 | 0 | 0.0005 | 0 | 0.0055 | 0.0005 | 0.0039 | 0 | 0.0008 | 0.0006 | 0.0001 | 0.0001 |
Cytoplasmic Foci | 0.0201 | 0.0008 | 0.0142 | 0.0204 | 0.0144 | 0.0003 | 0.0097 | 0.0001 | 0.0077 | 0 | 0.0293 | 0.0023 | 0.0046 | 0 | 0.0009 | 0.0033 | 0 | 0.0002 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0.0004 | 0 | 0 |
Endoplasmic Reticulum | 0.0025 | 0 | 0.002 | 0.0074 | 0.0027 | 0.001 | 0.0004 | 0 | 0.001 | 0 | 0.003 | 0.0002 | 0.0027 | 0 | 0.0006 | 0.0007 | 0 | 0.0001 |
Endosome | 0.0236 | 0.0011 | 0.0117 | 0.0624 | 0.0558 | 0.0029 | 0.0056 | 0 | 0.009 | 0 | 0.0409 | 0.0088 | 0.0121 | 0 | 0.0018 | 0.0022 | 0 | 0.0002 |
Golgi | 0.0099 | 0.0011 | 0.0055 | 0.0078 | 0.0193 | 0.0007 | 0.0047 | 0.0001 | 0.0029 | 0 | 0.0194 | 0.0043 | 0.0026 | 0 | 0.0005 | 0.0024 | 0 | 0.0001 |
Lipid Particles | 0.0152 | 0.001 | 0.0104 | 0.0066 | 0.0445 | 0.0027 | 0.0126 | 0 | 0.0098 | 0 | 0.0615 | 0.0062 | 0.0045 | 0 | 0.0009 | 0.0036 | 0 | 0.0005 |
Mitochondria | 0.0106 | 0.0111 | 0.009 | 0.0111 | 0.0346 | 0.0123 | 0.0094 | 0.0001 | 0.0023 | 0.0001 | 0.0283 | 0.002 | 0.0016 | 0.0001 | 0.0014 | 0.0054 | 0.0005 | 0.0002 |
None | 0.0043 | 0 | 0.0013 | 0.0034 | 0.0006 | 0.0002 | 0.0004 | 0 | 0.0004 | 0 | 0.012 | 0.0001 | 0.003 | 0 | 0.0014 | 0.0004 | 0 | 0.0001 |
Nuclear Periphery | 0.0116 | 0.0012 | 0.0051 | 0.0282 | 0.0066 | 0.0174 | 0.0029 | 0.0004 | 0.0067 | 0.0003 | 0.0185 | 0.0013 | 0.0081 | 0.0002 | 0.0032 | 0.0144 | 0.0013 | 0.0008 |
Nucleolus | 0.0353 | 0.0181 | 0.03 | 0.0086 | 0.1245 | 0.099 | 0.0297 | 0.0199 | 0.0238 | 0.0141 | 0.0974 | 0.0917 | 0.032 | 0.0233 | 0.0193 | 0.0367 | 0.1403 | 0.0823 |
Nucleus | 0.7901 | 0.9635 | 0.863 | 0.6907 | 0.6089 | 0.8485 | 0.8942 | 0.9688 | 0.917 | 0.9631 | 0.5688 | 0.8747 | 0.8823 | 0.9754 | 0.9547 | 0.8855 | 0.854 | 0.9059 |
Peroxisomes | 0.0071 | 0.0005 | 0.0058 | 0.0074 | 0.0078 | 0.0004 | 0.0048 | 0.0001 | 0.0038 | 0 | 0.011 | 0.0017 | 0.002 | 0 | 0.0003 | 0.0149 | 0 | 0.0001 |
Punctate Nuclear | 0.0131 | 0.0003 | 0.0043 | 0.0608 | 0.009 | 0.0035 | 0.0027 | 0.0003 | 0.0024 | 0.0222 | 0.0474 | 0.0028 | 0.0148 | 0.0008 | 0.0016 | 0.002 | 0.0023 | 0.0058 |
Vacuole | 0.0036 | 0.0004 | 0.0018 | 0.0102 | 0.0071 | 0.003 | 0.0007 | 0 | 0.0007 | 0 | 0.0066 | 0.0006 | 0.0023 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0002 |
Vacuole Periphery | 0.0011 | 0.0002 | 0.0006 | 0.0065 | 0.0036 | 0.0018 | 0.0005 | 0 | 0.0005 | 0 | 0.0038 | 0.0005 | 0.0008 | 0 | 0.0002 | 0.0005 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 28.7345 | 25.6869 | 28.5912 | 27.8806 | 18.6278 | 43.0779 | 24.6734 | 26.294 | 28.823 | 27.0397 |
Translational Efficiency | 1.491 | 1.0325 | 0.9278 | 1.4166 | 1.5507 | 1.1287 | 0.9635 | 0.8996 | 1.2057 | 1.0578 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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