Standard name
Human Ortholog
Description Subunit of Snt2C complex, RING finger ubiquitin ligase (E3); physically associates with Ecm5p and Rpd3p; along with Ecm5p, recruits Rpd3p to small number of promoters; colocalizes with Ecm5p, independently of Rpd3p, to promoters of stress response genes upon oxidative stress; involved in ubiquitination, degradation of excess histones; interacts with Ubc4p; role in regulating genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0.05 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 – – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.63 0.7 0.58 0.53 0.4 – 0.33 0.15 0.2 – – 0.54 0.63 0.67 0.94 0.92 0.92 0.4 0.51 0.47 0.46 0.56 0.58
Endoplasmic Reticulum 0.05 0 0 0 0 0 – 0 0 0 – – 0 0 0 0.05 0 0.07 0 0 0 0 0 0
Endosome 0 0 0.08 0.09 0.12 0 – 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 0.38 0.11 0.24 0.17 0.52 – 0.59 0.76 0.69 – – 0 0 0 0 0 0 0 0 0.16 0.1 0.12 0.08
Nucleus 0.1 0.06 0 0 0 0.08 – 0.12 0.17 0.24 – – 0.15 0 0.07 0 0 0 0.2 0.13 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 – – 0 0 0 0 0 0 0 0.06 0.05 0 0 0
Vac/Vac Membrane 0.3 0.09 0.27 0.24 0.33 0.21 – 0.11 0.21 0.14 – – 0.33 0.37 0.3 0.06 0 0.05 0.26 0.18 0.09 0.29 0.17 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 – 0 0 0 – – 1 0 0 0 1 0 0 2 3 0 2 4
Bud 0 0 1 3 0 8 – 10 18 6 – – 0 0 0 0 0 0 0 4 3 2 3 4
Bud Neck 0 1 0 1 1 0 – 0 0 0 – – 0 1 0 0 1 0 0 2 1 1 1 3
Bud Site 0 0 0 0 0 2 – 3 5 9 – – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 – – 0 0 1 1 2 2 0 0 0 0 0 2
Cytoplasm 4 189 77 94 126 115 – 133 53 80 – – 108 115 224 159 168 156 38 189 74 103 160 252
Endoplasmic Reticulum 1 1 1 0 0 1 – 2 0 0 – – 5 1 7 9 6 12 0 0 4 4 5 6
Endosome 0 1 9 15 28 3 – 15 2 4 – – 5 2 5 1 2 1 1 5 2 1 1 4
Golgi 0 0 0 0 0 0 – 0 0 0 – – 0 0 0 0 2 0 0 1 0 0 1 1
Mitochondria 8 113 12 39 40 148 – 242 271 268 – – 0 0 0 5 3 6 4 15 25 22 34 34
Nucleus 2 17 1 5 6 23 – 48 61 92 – – 29 8 22 1 2 0 18 48 5 9 7 7
Nuclear Periphery 0 0 0 0 0 0 – 0 0 1 – – 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 1 – – 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 – – 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 1 1 1 – 4 5 3 – – 0 2 0 0 0 1 4 20 8 4 12 12
Vac/Vac Membrane 6 27 30 39 78 61 – 43 75 54 – – 65 67 100 10 6 9 24 67 15 65 49 85
Unique Cell Count 20 298 110 163 238 286 407 358 391 199 183 333 170 183 169 96 373 158 223 288 432
Labelled Cell Count 21 349 133 198 280 362 500 490 518 213 198 359 186 193 187 96 373 158 223 288 432


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 3.3 4.4 4.0 4.2 3.5 – 3.9 3.5 3.8 – – 5.5 5.4 5.4 5.4 5.0 5.1 4.7 5.0 5.3
Std Deviation (1e-4) 0.2 0.7 1.1 0.6 0.7 0.8 – 1.3 1.0 1.1 – – 1.0 1.1 1.0 1.0 0.9 1.0 0.9 1.2 1.3
Intensity Change (Log2) – – – -0.12 -0.07 -0.34 – -0.16 -0.34 -0.22 – – 0.32 0.31 0.3 0.31 0.19 0.23 0.11 0.19 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -5.0 -6.1 -5.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.5071 -1.3785 -1.0581 -1.4994 -1.5595 -1.1567 0.1314 0.0454 -0.2647 0.0991 -0.4895 0.0523 -1.0551 -1.0412 -1.0799 -0.7693 -1.2423 -1.2918
Actin 0.002 0.0004 0.0056 0.0004 0.0002 0.002 0.0403 0.0003 0.0183 0.006 0.0641 0.002 0.0088 0.0034 0.0002 0.0013 0.0002 0.0003
Bud 0.0004 0.0001 0.0016 0 0 0.0013 0.0017 0.0003 0.0004 0.001 0.0402 0.0002 0.0003 0.0001 0.0001 0.0001 0 0.0001
Bud Neck 0.0002 0.0001 0.0008 0.0002 0.0006 0.0018 0.0038 0.0004 0.0035 0.0037 0.0015 0.0032 0.0017 0.0002 0.0002 0.0023 0.0003 0.0015
Bud Periphery 0.0004 0 0.0018 0 0 0.0023 0.0028 0.0002 0.0005 0.0013 0.0162 0.0006 0.0004 0 0 0.0001 0 0.0001
Bud Site 0.0013 0.0005 0.0072 0.0001 0.0001 0.0004 0.0087 0.0024 0.0044 0.0052 0.0047 0.0004 0.0041 0.0006 0.0004 0.0072 0.0001 0.0002
Cell Periphery 0 0 0.0002 0 0 0.0001 0.0003 0.0001 0.0002 0.0002 0.0004 0.0001 0.0003 0.0001 0 0.0001 0 0.0001
Cytoplasm 0.2015 0.1907 0.2101 0.1356 0.3368 0.2284 0.2035 0.2195 0.2239 0.2125 0.2368 0.2969 0.2048 0.2532 0.2384 0.1202 0.267 0.2388
Cytoplasmic Foci 0.016 0.0121 0.0145 0.0121 0.0048 0.0114 0.0467 0.0156 0.0128 0.0275 0.0051 0.0128 0.0356 0.0078 0.0065 0.0532 0.0061 0.0046
Eisosomes 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0001 0.0003 0.0001 0.0014 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0028 0.0027 0.0042 0.0029 0.0064 0.0028 0.0052 0.0029 0.0026 0.0022 0.0036 0.0036 0.0048 0.0037 0.0064 0.0041 0.0108 0.0035
Endosome 0.0087 0.0068 0.0105 0.0049 0.0152 0.0048 0.0213 0.0059 0.0081 0.0363 0.009 0.0076 0.0271 0.0085 0.0193 0.0039 0.0055 0.0053
Golgi 0.0016 0.0036 0.0005 0.0002 0.0001 0.0004 0.0058 0.0003 0.0066 0.0217 0.0128 0.0022 0.0184 0.0123 0.0014 0.001 0.0001 0.0001
Lipid Particles 0.0018 0.0006 0.0012 0.0008 0.0008 0.0008 0.0091 0.0003 0.0027 0.0118 0.0072 0.003 0.0202 0.0007 0.0003 0.0049 0.0005 0.0005
Mitochondria 0.0008 0.0017 0.001 0.0011 0.0008 0.0007 0.0021 0.0008 0.0083 0.0067 0.0171 0.0079 0.0156 0.0008 0.0008 0.0009 0.0008 0.0009
None 0.5962 0.6769 0.5226 0.5541 0.4524 0.5586 0.4389 0.5549 0.4435 0.3853 0.431 0.3876 0.5654 0.593 0.5749 0.6715 0.5388 0.5045
Nuclear Periphery 0.0088 0.0056 0.0133 0.0153 0.025 0.0068 0.0134 0.0083 0.0136 0.0131 0.0067 0.0081 0.0039 0.0076 0.0106 0.0123 0.0139 0.0154
Nucleolus 0.0011 0.001 0.003 0.0012 0.0013 0.0018 0.0035 0.0026 0.002 0.005 0.0016 0.0028 0.0032 0.0005 0.0009 0.0018 0.0013 0.0024
Nucleus 0.1341 0.0867 0.17 0.2551 0.1263 0.1448 0.1229 0.1641 0.2067 0.2195 0.1259 0.2215 0.0603 0.1009 0.122 0.0942 0.1387 0.2002
Peroxisomes 0.0018 0.0007 0.0006 0.0003 0.0001 0.0008 0.0208 0.0011 0.0104 0.0044 0.0057 0.0088 0.0158 0.0001 0.0002 0.0014 0.0001 0.0001
Punctate Nuclear 0.0178 0.0083 0.028 0.014 0.0224 0.0288 0.0454 0.0163 0.0286 0.0317 0.0068 0.0277 0.0034 0.0041 0.0153 0.0179 0.0127 0.0183
Vacuole 0.002 0.001 0.0026 0.0011 0.006 0.001 0.0028 0.0032 0.002 0.0037 0.0014 0.0022 0.0051 0.0019 0.0014 0.0009 0.0027 0.0024
Vacuole Periphery 0.0004 0.0002 0.0005 0.0005 0.0008 0.0002 0.0007 0.0005 0.0005 0.0012 0.0008 0.0006 0.0007 0.0004 0.0005 0.0004 0.0004 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.8238 5.152 7.9936 10.8616 8.4913 9.1638 12.0759 12.1457 10.7612 12.3676
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1594 214 2531 2141 1708 2609 94 2181 3302 2823 2625 4322

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 666.15 708.02 815.24 749.64 638.11 700.05 863.31 740.73 651.65 700.65 816.96 745.14
Standard Deviation 70.52 96.56 100.60 92.96 86.95 84.66 119.03 103.73 80.67 85.64 101.71 98.64
Intensity Change Log 2 — 0.087943 0.291378 0.170351 — 0.133653 0.436074 0.215143 — 0.110488 0.363984 0.192439

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000030 0.000261 0.000249 0.000236 0.000060 0.000217 0.000663 0.000150 0.000046 0.000221 0.000263 0.000193
Bud Neck 0.004669 0.007929 0.007803 0.006832 0.002561 0.018276 0.004760 0.007055 0.003578 0.017492 0.007694 0.006945
Bud Site 0.000546 0.001537 0.003417 0.004852 0.000773 0.006086 0.020172 0.008722 0.000664 0.005741 0.004017 0.006805
Cell Periphery 0.000070 0.000148 0.000097 0.000135 0.000103 0.000090 0.000188 0.001029 0.000087 0.000094 0.000100 0.000586
Cytoplasm 0.435237* 0.188083* 0.043245 0.185047* 0.374802* 0.328866* 0.083787 0.227851* 0.403976* 0.318194* 0.044697 0.206647*
Cytoplasmic Foci 0.101554 0.080908 0.000522 0.003463 0.066590 0.065492 0.004817 0.008674 0.083469 0.066661 0.000676 0.006093
Eisosomes 0.000051 0.000085 0.000013 0.000022 0.000049 0.000042 0.000068 0.000200 0.000050 0.000045 0.000015 0.000112
Endoplasmic Reticulum 0.000578 0.002948 0.002969 0.002574 0.000660 0.001294 0.008250 0.001276 0.000621 0.001419 0.003158 0.001919
Endosome 0.003216 0.005414 0.000429 0.000890 0.002135 0.005399 0.008134 0.001790 0.002657 0.005400 0.000705 0.001344
Golgi 0.000462 0.002502 0.000184 0.000059 0.000525 0.001052 0.003361 0.000777 0.000495 0.001162 0.000297 0.000421
Lipid Particles 0.005654 0.009316 0.000451 0.000679 0.005728 0.005117 0.002722 0.001019 0.005692 0.005436 0.000532 0.000851
Mitochondria 0.003196 0.011772 0.001060 0.001439 0.001819 0.010597 0.001668 0.001548 0.002484 0.010686 0.001081 0.001494
Mitotic Spindle 0.001441 0.003791 0.001898 0.008879 0.002770 0.012608 0.031263 0.006365 0.002128 0.011940 0.002949 0.007610
None 0.006388 0.019583 0.003861 0.007780 0.015825 0.006193 0.002949 0.019199 0.011269 0.007208 0.003829 0.013542
Nuclear Periphery 0.000982 0.002325 0.001623 0.002403 0.001101 0.002393 0.002288 0.001502 0.001043 0.002388 0.001647 0.001948
Nuclear Periphery Foci 0.000322 0.001116 0.000187 0.000392 0.000588 0.000353 0.002145 0.000339 0.000459 0.000411 0.000257 0.000365
Nucleolus 0.001423 0.010792 0.000916 0.001593 0.003420 0.004090 0.001283 0.002173 0.002456 0.004598 0.000929 0.001886
Nucleus 0.415766* 0.620516* 0.927404* 0.760966* 0.511026* 0.504585* 0.747343* 0.699827* 0.465040* 0.513373* 0.920956* 0.730114*
Peroxisomes 0.001785 0.005382 0.000101 0.000385 0.001952 0.005577 0.000378 0.000920 0.001871 0.005563 0.000111 0.000655
Vacuole 0.016162 0.024193 0.003478 0.011154 0.007187 0.020115 0.059758 0.009174 0.011519 0.020424 0.005494 0.010155
Vacuole Periphery 0.000468 0.001398 0.000093 0.000219 0.000326 0.001558 0.014006 0.000409 0.000395 0.001546 0.000592 0.000315

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.42 -12.71 -7.51 -0.01 0.50 -2.27 -5.27 -6.07 0.16 4.46 -2.82 -12.72 -9.59 -0.40 2.99
Bud Neck -2.48 -5.12 -4.09 -0.06 1.04 -13.89 -3.01 -7.17 7.48 -0.66 -13.35 -7.13 -7.95 8.08 1.04
Bud Site -3.86 -4.67 -5.49 -3.67 -1.19 -7.25 -1.91 -5.94 -1.47 0.82 -7.61 -4.86 -7.94 -0.82 -2.49
Cell Periphery -3.90 -1.89 -6.10 1.07 -2.38 2.57 -2.41 -5.83 -5.96 -5.20 -1.03 -0.66 -6.25 -6.15 -6.03
Cytoplasm 15.69 60.40 33.89 1.03 -26.11 5.57 16.74 20.20 16.80 -7.41 12.95 74.00 37.58 21.48 -38.77
Cytoplasmic Foci 1.64 27.78 26.84 8.69 -8.48 0.41 21.32 20.32 24.01 -3.91 4.95 35.87 33.37 26.70 -13.61
Eisosomes -4.46 16.21 10.40 7.18 -5.73 2.78 -2.88 -2.71 -2.89 -1.86 1.95 17.52 -2.12 -2.32 -3.64
Endoplasmic Reticulum -6.50 -19.04 -14.25 -0.64 2.06 -3.72 -7.04 -10.16 -3.17 6.14 -5.21 -20.11 -17.39 -6.46 7.34
Endosome -2.32 8.25 6.32 4.85 -5.81 -6.59 -1.66 1.37 7.11 1.93 -5.98 9.80 5.31 9.27 -4.50
Golgi -1.09 1.89 2.83 1.33 0.61 -1.95 -0.67 -0.27 1.12 0.56 -2.87 1.84 0.64 3.17 -0.89
Lipid Particles -2.72 10.48 10.21 6.39 -2.06 1.10 3.17 11.17 11.93 2.86 0.36 14.89 15.12 13.70 -2.38
Mitochondria -2.14 2.62 2.20 2.63 -1.51 -10.62 1.49 0.72 10.90 -0.92 -9.36 3.31 2.30 11.41 -2.16
Mitotic Spindle -1.14 -1.33 -5.70 -1.47 -5.10 -5.43 -2.60 -3.89 1.69 2.11 -6.39 -1.80 -6.80 0.88 -5.04
None -2.74 3.03 -1.06 2.51 -5.41 6.57 8.42 -0.38 -6.59 -8.32 4.14 8.86 -0.70 -4.91 -9.75
Nuclear Periphery -5.27 -10.40 -15.18 -2.85 -7.00 -8.02 -5.35 -8.41 1.10 2.71 -9.52 -11.17 -16.79 -1.75 -4.69
Nuclear Periphery Foci -3.42 0.39 -3.91 0.25 -3.96 2.46 -1.76 0.27 -1.92 1.78 0.87 0.19 -2.79 -3.47 -2.30
Nucleolus -5.29 2.32 -3.19 4.33 -3.90 -0.64 3.68 0.27 0.91 -3.18 -5.08 5.96 -1.43 3.06 -6.09
Nucleus -9.00 -71.88 -40.27 -6.94 25.97 0.73 -6.18 -21.57 -24.46 0.29 -6.21 -79.01 -42.35 -31.71 35.42
Peroxisomes -3.89 7.60 5.66 5.28 -2.18 -6.18 5.76 3.09 8.27 -1.70 -7.28 9.83 5.63 9.96 -3.23
Vacuole -2.78 11.04 -0.53 2.51 -9.45 -11.83 -5.02 -6.05 4.67 4.60 -8.47 3.94 -4.16 4.40 -6.84
Vacuole Periphery -2.64 3.33 2.30 3.68 -2.57 -5.35 -1.01 -0.37 5.12 1.00 -5.31 0.67 1.55 6.01 -0.04
Standard name
Human Ortholog
Description Subunit of Snt2C complex, RING finger ubiquitin ligase (E3); physically associates with Ecm5p and Rpd3p; along with Ecm5p, recruits Rpd3p to small number of promoters; colocalizes with Ecm5p, independently of Rpd3p, to promoters of stress response genes upon oxidative stress; involved in ubiquitination, degradation of excess histones; interacts with Ubc4p; role in regulating genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress
Localization
Cell Percentages nucleus (22%), cytoplasm (7%), mixed (50%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Snt2

Snt2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snt2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available