Standard name | |
---|---|
Human Ortholog | |
Description | Essential beta'-coat protein of the COPI coatomer; involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 47 | 61 | 56 | 32 | 24 | 43 | 39 | – | 35 | 28 | 77 | 24 | 27 | 13 | 17 | 50 | 14 | 0 | 8 | 33 | 11 | 16 | 35 |
Bud | 0 | 2 | 5 | 4 | 1 | 3 | 4 | 9 | – | 11 | 16 | 8 | 1 | 1 | 0 | 1 | 1 | 3 | 0 | 1 | 1 | 0 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | – | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 3 | 1 | 3 | 3 | 3 | 1 | – | 0 | 3 | 6 | 1 | 1 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 13 | 12 | 13 | 11 | 10 | 14 | 15 | – | 16 | 4 | 19 | 11 | 11 | 14 | 17 | 34 | 20 | 0 | 4 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 3 | 24 | 12 | 11 | 6 | 10 | 14 | 6 | – | 12 | 6 | 28 | 20 | 19 | 17 | 7 | 18 | 7 | 0 | 5 | 0 | 1 | 2 | 4 |
Endosome | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | – | 0 | 0 | 6 | 1 | 1 | 1 | 14 | 11 | 3 | 0 | 4 | 5 | 3 | 2 | 4 |
Golgi | 36 | 135 | 401 | 263 | 303 | 104 | 187 | 218 | – | 135 | 38 | 154 | 264 | 260 | 255 | 81 | 257 | 173 | 57 | 207 | 467 | 203 | 353 | 461 |
Mitochondria | 15 | 122 | 337 | 279 | 322 | 475 | 626 | 649 | – | 643 | 447 | 607 | 30 | 30 | 15 | 27 | 39 | 16 | 2 | 7 | 8 | 11 | 11 | 9 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 1 | – | 1 | 2 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | – | 1 | 2 | 2 | 0 | 0 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 3 | 10 | 25 | 17 | 38 | 52 | 101 | – | 53 | 42 | 55 | 3 | 3 | 2 | 14 | 19 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 4 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 7 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Vac/Vac Membrane | 9 | 21 | 28 | 15 | 37 | 14 | 51 | 46 | – | 34 | 15 | 22 | 45 | 45 | 65 | 37 | 59 | 27 | 1 | 29 | 4 | 4 | 8 | 4 |
Unique Cell Count | 43 | 220 | 514 | 383 | 480 | 533 | 769 | 842 | 743 | 493 | 752 | 295 | 291 | 299 | 181 | 378 | 223 | 64 | 272 | 533 | 238 | 401 | 529 | |
Labelled Cell Count | 66 | 368 | 870 | 667 | 733 | 687 | 1000 | 1089 | 945 | 606 | 987 | 400 | 398 | 382 | 233 | 496 | 276 | 64 | 272 | 533 | 238 | 401 | 529 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 36.4 | 34.2 | 30.0 | 28.1 | 27.4 | 23.8 | 23.4 | 22.7 | – | 21.7 | 19.6 | 20.7 | 34.2 | 34.2 | 37.1 | 15.1 | 22.0 | 28.2 | 35.0 | 36.2 | 37.9 |
Std Deviation (1e-4) | 7.1 | 7.5 | 6.6 | 6.7 | 5.1 | 5.2 | 5.1 | 5.2 | – | 5.5 | 4.7 | 5.0 | 8.3 | 8.3 | 10.1 | 5.6 | 9.3 | 10.5 | 6.3 | 6.7 | 7.6 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.13 | -0.33 | -0.36 | -0.4 | – | -0.47 | -0.61 | -0.54 | 0.19 | 0.19 | 0.31 | -0.99 | -0.45 | -0.09 | 0.22 | 0.27 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.2 | -2.8 | -4.4 | -4.0 | -4.9 | – | -4.7 | -3.5 | -0.9 | -1.7 | -1.1 | -3.6 | -0.9 | 0.6 | -2.3 |
Bud | 0 | 0 | 0 | 0 | 0.2 | – | 0.8 | 2.5 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.0 | 0 | -0.5 | -0.6 | -0.7 | – | -0.2 | -1.9 | 0.2 | 1.1 | 1.2 | 1.8 | 4.1 | 4.4 | 4.1 |
Endoplasmic Reticulum | 0.5 | -1.3 | -0.5 | -0.6 | -2.5 | – | -0.9 | -1.3 | 1.4 | 3.1 | 3.0 | 2.5 | 0 | 2.0 | 0.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 0 |
Golgi | -3.2 | -5.2 | -18.9 | -18.9 | -18.7 | – | -21.1 | -22.5 | -20.3 | 4.1 | 4.0 | 2.5 | -8.3 | -3.4 | -0.1 |
Mitochondria | 2.3 | 0.5 | 9.1 | 6.4 | 4.6 | – | 8.8 | 9.6 | 6.1 | -15.2 | -15.1 | -16.8 | -11.7 | -16.5 | -14.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3.5 | 1.5 | 4.0 | 3.9 | 6.5 | – | 4.1 | 4.7 | 4.3 | 0 | 0 | 0 | 3.7 | 2.6 | 2.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.1 | 1.4 | -2.3 | 0.9 | 0 | – | -0.7 | -1.9 | -2.3 | 4.7 | 4.8 | 7.0 | 6.0 | 5.1 | 3.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 26.8834 | 33.475 | 31.5703 | 28.1366 | 27.2891 | 31.3948 | 23.986 | 34.0803 | 32.3313 | 29.7964 | 30.2942 | 31.8712 | 26.4887 | 34.8918 | 31.907 | 29.6598 | 26.2146 | 33.2399 |
Actin | 0.0287 | 0.0112 | 0.0077 | 0.0079 | 0.0063 | 0.0019 | 0.0403 | 0.0141 | 0.0267 | 0.0484 | 0.0177 | 0.0101 | 0.0397 | 0.0041 | 0.0241 | 0.0048 | 0.0058 | 0.0018 |
Bud | 0.0003 | 0.0008 | 0.0002 | 0.0009 | 0.0002 | 0 | 0.0006 | 0.0001 | 0.0003 | 0.0004 | 0.0003 | 0.0001 | 0.0006 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0 |
Bud Neck | 0.0003 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0022 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0 |
Bud Periphery | 0.0003 | 0.0005 | 0.0002 | 0.0015 | 0.0003 | 0 | 0.0008 | 0.0001 | 0.0004 | 0.0005 | 0.0003 | 0.0001 | 0.0009 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0 |
Bud Site | 0.0012 | 0.0026 | 0.0002 | 0.0039 | 0.0002 | 0 | 0.002 | 0.0003 | 0.001 | 0.0012 | 0.0013 | 0.0002 | 0.0015 | 0.0001 | 0.0022 | 0.0001 | 0.0011 | 0 |
Cell Periphery | 0.0003 | 0.0011 | 0.0002 | 0.0009 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0001 |
Cytoplasm | 0.0023 | 0.0038 | 0.002 | 0.012 | 0.0014 | 0.0002 | 0.0054 | 0.0003 | 0.0005 | 0.0008 | 0.0014 | 0.0002 | 0.0012 | 0.0009 | 0.0025 | 0.0007 | 0.0105 | 0.0001 |
Cytoplasmic Foci | 0.0259 | 0.0134 | 0.0088 | 0.0081 | 0.0266 | 0.0045 | 0.0191 | 0.0067 | 0.0126 | 0.0084 | 0.02 | 0.0033 | 0.0161 | 0.009 | 0.0085 | 0.0111 | 0.0171 | 0.0041 |
Eisosomes | 0.0003 | 0.0012 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0006 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0014 | 0.0015 | 0.0008 | 0.0046 | 0.0007 | 0.0005 | 0.0044 | 0.0003 | 0.0007 | 0.0016 | 0.0009 | 0.0008 | 0.0011 | 0.0004 | 0.0014 | 0.001 | 0.0036 | 0.0003 |
Endosome | 0.0876 | 0.1243 | 0.0976 | 0.094 | 0.0838 | 0.0642 | 0.0665 | 0.0537 | 0.0713 | 0.1787 | 0.0505 | 0.0447 | 0.0826 | 0.0988 | 0.0737 | 0.0689 | 0.156 | 0.0405 |
Golgi | 0.7135 | 0.7528 | 0.7963 | 0.6498 | 0.7581 | 0.8829 | 0.5888 | 0.8461 | 0.74 | 0.6404 | 0.7862 | 0.8626 | 0.6783 | 0.8061 | 0.7518 | 0.7059 | 0.7289 | 0.8636 |
Lipid Particles | 0.015 | 0.0123 | 0.0086 | 0.0106 | 0.0139 | 0.0027 | 0.0321 | 0.0035 | 0.0253 | 0.014 | 0.0198 | 0.0059 | 0.0199 | 0.0031 | 0.0055 | 0.0246 | 0.0226 | 0.0083 |
Mitochondria | 0.0657 | 0.0272 | 0.0302 | 0.0715 | 0.0627 | 0.0294 | 0.1829 | 0.0637 | 0.0895 | 0.0362 | 0.0253 | 0.051 | 0.1213 | 0.034 | 0.0883 | 0.0688 | 0.0088 | 0.0626 |
None | 0.0025 | 0.0001 | 0.0006 | 0.0027 | 0.0015 | 0 | 0.0049 | 0 | 0.0002 | 0.0002 | 0.0016 | 0.0001 | 0.0025 | 0 | 0.0066 | 0.0004 | 0.0046 | 0.0001 |
Nuclear Periphery | 0.005 | 0.0016 | 0.0019 | 0.0035 | 0.0007 | 0.0026 | 0.0091 | 0.0001 | 0.0005 | 0.0025 | 0.001 | 0.001 | 0.0014 | 0.0002 | 0.0011 | 0.0014 | 0.0024 | 0.0003 |
Nucleolus | 0.0035 | 0.0002 | 0.0003 | 0.001 | 0.0007 | 0 | 0.0013 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0013 |
Nucleus | 0.0057 | 0.0004 | 0.0004 | 0.0046 | 0.0005 | 0 | 0.0076 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0007 | 0.0001 |
Peroxisomes | 0.0072 | 0.0004 | 0.0004 | 0.0294 | 0.0021 | 0 | 0.0031 | 0.0016 | 0.0022 | 0.0011 | 0.0115 | 0.0004 | 0.0042 | 0.0001 | 0.002 | 0.0021 | 0.0019 | 0.0006 |
Punctate Nuclear | 0.0037 | 0.0001 | 0.0004 | 0.0006 | 0.0004 | 0 | 0.0056 | 0 | 0.0002 | 0.0001 | 0.0017 | 0.0001 | 0.0023 | 0 | 0.0002 | 0.0001 | 0.0031 | 0.0001 |
Vacuole | 0.0062 | 0.0197 | 0.0117 | 0.0399 | 0.0056 | 0.0012 | 0.0046 | 0.0008 | 0.0049 | 0.0149 | 0.0059 | 0.0033 | 0.004 | 0.0138 | 0.0055 | 0.0143 | 0.0083 | 0.0014 |
Vacuole Periphery | 0.0234 | 0.0246 | 0.0311 | 0.0521 | 0.0339 | 0.0096 | 0.0192 | 0.0082 | 0.0223 | 0.0495 | 0.0536 | 0.0157 | 0.0183 | 0.0288 | 0.0246 | 0.0944 | 0.0235 | 0.0146 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 173.3626 | 167.3515 | 150.2246 | 174.8161 | 176.8172 | 138.3655 | 169.2089 | 167.9034 | 175.4201 | 191.5429 |
Translational Efficiency | 1.1205 | 1.1298 | 1.1008 | 1.1124 | 1.0082 | 1.1914 | 1.1108 | 0.8549 | 0.9033 | 0.9354 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
157 | 1047 | 343 | 163 | 1670 | 329 | 2136 | 1815 | 1827 | 1376 | 2479 | 1978 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2625.42 | 2451.72 | 3499.75 | 4129.31 | 1763.32 | 2378.74 | 3122.72 | 3434.98 | 1837.40 | 2434.27 | 3174.89 | 3492.20 |
Standard Deviation | 496.73 | 493.62 | 488.30 | 794.26 | 399.22 | 427.34 | 521.31 | 682.02 | 474.61 | 479.62 | 533.01 | 717.82 |
Intensity Change Log 2 | — | -0.098754 | 0.414704 | 0.653352 | — | 0.431903 | 0.824509 | 0.962007 | — | 0.138354 | 0.593563 | 0.785398 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.010307 | 0.005660 | 0.011802 | 0.001208 | 0.005915 | 0.005621 | 0.002225 | 0.002871 | 0.006292 | 0.005651 | 0.003550 | 0.002734 |
Bud Neck | 0.001418 | 0.000493 | 0.002918 | 0.000782 | 0.000369 | 0.001017 | 0.000666 | 0.000556 | 0.000459 | 0.000618 | 0.000977 | 0.000575 |
Bud Site | 0.002765 | 0.000381 | 0.000823 | 0.002780 | 0.000731 | 0.000719 | 0.000573 | 0.000924 | 0.000906 | 0.000462 | 0.000608 | 0.001077 |
Cell Periphery | 0.001965 | 0.000351 | 0.000773 | 0.004374 | 0.000562 | 0.000228 | 0.000503 | 0.000549 | 0.000682 | 0.000321 | 0.000540 | 0.000864 |
Cytoplasm | 0.000403 | 0.000114 | 0.000042 | 0.000303 | 0.000645 | 0.002190 | 0.000083 | 0.000182 | 0.000624 | 0.000610 | 0.000078 | 0.000192 |
Cytoplasmic Foci | 0.017165 | 0.013372 | 0.007728 | 0.008367 | 0.021088 | 0.017849 | 0.007901 | 0.006378 | 0.020751 | 0.014442 | 0.007877 | 0.006542 |
Eisosomes | 0.000312 | 0.000332 | 0.000895 | 0.000059 | 0.000189 | 0.000115 | 0.000099 | 0.000177 | 0.000200 | 0.000280 | 0.000209 | 0.000168 |
Endoplasmic Reticulum | 0.005930 | 0.003110 | 0.005433 | 0.003091 | 0.003628 | 0.001408 | 0.001586 | 0.002063 | 0.003826 | 0.002703 | 0.002118 | 0.002148 |
Endosome | 0.026891 | 0.007516 | 0.006993 | 0.019661 | 0.011804 | 0.016413 | 0.010564 | 0.012649 | 0.013100 | 0.009643 | 0.010070 | 0.013227 |
Golgi | 0.764854* | 0.857702* | 0.762261* | 0.764092* | 0.809252* | 0.845640* | 0.828515* | 0.865885* | 0.805437* | 0.854818* | 0.819347* | 0.857497* |
Lipid Particles | 0.029297 | 0.038271 | 0.087486 | 0.042161 | 0.014896 | 0.018090 | 0.032641 | 0.022135 | 0.016133 | 0.033446 | 0.040229 | 0.023785 |
Mitochondria | 0.001554 | 0.023203 | 0.008907 | 0.000970 | 0.081399 | 0.018121 | 0.011697 | 0.006517 | 0.074538 | 0.021988 | 0.011311 | 0.006060 |
Mitotic Spindle | 0.002007 | 0.000149 | 0.000103 | 0.000876 | 0.000194 | 0.002047 | 0.000290 | 0.000621 | 0.000350 | 0.000603 | 0.000264 | 0.000642 |
None | 0.000182 | 0.000861 | 0.000679 | 0.000103 | 0.001384 | 0.000244 | 0.000266 | 0.000189 | 0.001281 | 0.000713 | 0.000324 | 0.000182 |
Nuclear Periphery | 0.001431 | 0.000446 | 0.001445 | 0.000364 | 0.000231 | 0.001169 | 0.000841 | 0.000623 | 0.000334 | 0.000619 | 0.000924 | 0.000601 |
Nuclear Periphery Foci | 0.001708 | 0.000421 | 0.001039 | 0.001994 | 0.000296 | 0.001271 | 0.000961 | 0.001142 | 0.000418 | 0.000624 | 0.000972 | 0.001213 |
Nucleolus | 0.000027 | 0.000371 | 0.000082 | 0.000017 | 0.000094 | 0.000035 | 0.000039 | 0.000265 | 0.000089 | 0.000291 | 0.000045 | 0.000245 |
Nucleus | 0.000031 | 0.000163 | 0.000045 | 0.000025 | 0.000069 | 0.000024 | 0.000017 | 0.000043 | 0.000066 | 0.000129 | 0.000021 | 0.000041 |
Peroxisomes | 0.000338 | 0.000070 | 0.000052 | 0.000045 | 0.000147 | 0.000097 | 0.000035 | 0.000053 | 0.000164 | 0.000076 | 0.000038 | 0.000052 |
Vacuole | 0.068999 | 0.007315 | 0.023124 | 0.100360 | 0.004704 | 0.021865 | 0.021835 | 0.022878 | 0.010229 | 0.010794 | 0.022013 | 0.029263 |
Vacuole Periphery | 0.062417 | 0.039700 | 0.077369 | 0.048367 | 0.042404 | 0.045838 | 0.078663 | 0.053299 | 0.044123 | 0.041168 | 0.078484 | 0.052893 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.78 | -0.22 | 1.52 | 4.83 | 3.95 | 0.14 | 3.87 | 2.96 | 1.33 | -1.02 | 0.54 | 2.56 | 3.32 | 3.13 | 1.17 |
Bud Neck | 2.76 | -2.37 | 1.70 | -1.61 | 3.78 | -1.58 | -1.73 | -1.19 | 1.08 | 0.60 | -0.99 | -2.96 | -0.81 | 0.16 | 2.21 |
Bud Site | 2.66 | 2.14 | 0.02 | -3.49 | -2.76 | 0.05 | 0.83 | -0.89 | -0.83 | -1.93 | 2.38 | 1.59 | -0.78 | -3.47 | -2.65 |
Cell Periphery | 2.89 | 2.12 | -1.78 | -3.22 | -2.88 | 2.69 | 0.39 | 0.08 | -6.25 | -0.50 | 2.49 | 1.00 | -1.13 | -3.94 | -2.42 |
Cytoplasm | 1.27 | 1.59 | 0.42 | -1.85 | -2.71 | -0.72 | 1.47 | 1.22 | 0.96 | -2.36 | 0.04 | 1.56 | 1.25 | 0.83 | -2.91 |
Cytoplasmic Foci | 0.86 | 2.06 | 1.79 | 2.32 | -0.34 | 1.56 | 14.58 | 17.01 | 5.84 | 2.66 | 5.69 | 14.07 | 15.87 | 9.41 | 2.39 |
Eisosomes | -0.12 | -2.41 | 1.60 | 5.17 | 4.51 | 2.48 | 5.33 | 0.19 | -1.01 | -1.39 | -1.84 | -0.29 | 0.58 | 1.73 | 0.70 |
Endoplasmic Reticulum | 1.97 | 0.23 | 1.83 | 0.02 | 1.39 | 6.85 | 6.65 | 4.77 | -2.61 | -2.07 | 3.21 | 4.58 | 5.11 | 2.00 | -0.10 |
Endosome | 4.86 | 4.96 | 1.36 | -3.35 | -3.47 | -2.33 | 1.42 | -0.84 | 1.85 | -2.24 | 3.74 | 3.55 | -0.14 | -3.71 | -3.52 |
Golgi | -4.39 | 0.07 | 0.06 | 4.31 | -0.00 | -2.64 | -2.47 | -7.38 | -1.53 | -5.65 | -6.19 | -1.87 | -6.94 | -0.30 | -5.88 |
Lipid Particles | -1.25 | -5.53 | -1.17 | -0.43 | 3.63 | -0.77 | -6.64 | -3.07 | -0.97 | 3.75 | -5.59 | -8.90 | -3.25 | 2.97 | 5.73 |
Mitochondria | -7.48 | -3.63 | 0.78 | 7.53 | 3.77 | 9.97 | 14.78 | 15.98 | 2.55 | 3.15 | 10.84 | 14.58 | 15.86 | 6.05 | 3.53 |
Mitotic Spindle | 2.44 | 2.50 | 1.32 | -1.80 | -1.92 | -1.32 | -1.46 | -1.71 | 0.98 | -1.30 | -0.71 | 0.97 | -1.22 | -0.15 | -1.60 |
None | -3.06 | -7.24 | 2.13 | 3.46 | 9.50 | 4.71 | 4.60 | 4.96 | 1.51 | 1.82 | 2.07 | 4.31 | 4.98 | 3.17 | 3.71 |
Nuclear Periphery | 1.37 | -0.02 | 1.48 | 1.02 | 5.46 | -1.70 | -8.36 | -2.62 | 0.94 | 1.28 | -1.87 | -6.41 | -1.80 | 0.04 | 2.07 |
Nuclear Periphery Foci | 2.63 | 1.34 | -0.53 | -4.95 | -2.88 | -2.17 | -5.10 | -3.57 | 0.21 | -0.72 | -1.55 | -4.54 | -3.57 | -2.45 | -1.04 |
Nucleolus | -1.00 | -3.81 | 1.86 | 1.02 | 4.56 | 1.41 | 1.35 | -0.74 | -0.99 | -0.98 | -0.75 | 1.17 | -0.74 | 0.06 | -0.94 |
Nucleus | -0.93 | -1.58 | 0.64 | 0.97 | 2.59 | 2.06 | 2.40 | 0.86 | -0.91 | -1.24 | -0.55 | 2.28 | 0.88 | 0.79 | -1.09 |
Peroxisomes | 1.56 | 1.67 | 1.71 | 1.16 | 0.44 | 1.20 | 3.58 | 2.67 | 1.25 | -1.07 | 2.46 | 3.93 | 3.14 | 1.00 | -0.98 |
Vacuole | 5.79 | 4.21 | -1.91 | -7.15 | -5.86 | -4.87 | -13.24 | -11.43 | -0.29 | -0.53 | -0.37 | -7.66 | -8.99 | -8.70 | -3.39 |
Vacuole Periphery | 2.87 | -1.54 | 1.47 | -1.39 | 3.45 | -0.64 | -10.81 | -3.53 | -1.36 | 7.26 | 1.01 | -10.92 | -2.98 | -3.90 | 7.89 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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