ORF | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 7 | 0 | 2 | 6 | 13 | 23 | 27 | 17 | 12 | 13 | 9 | 6 | 7 | 5 | 6 | 16 | 9 | 8 | 3 | 4 | 3 | 3 | 9 | 6 |
Bud | 1 | 0 | 9 | 16 | 16 | 31 | 48 | 63 | 28 | 24 | 20 | 26 | 5 | 6 | 13 | 6 | 4 | 10 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 3 | 2 | 3 | 5 | 8 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | – | – | – | – | – | – |
Cell Periphery | 20 | 1 | 15 | 24 | 30 | 38 | 57 | 44 | 33 | 54 | 35 | 49 | 44 | 68 | 76 | 18 | 6 | 18 | 2 | 0 | 8 | 1 | 6 | 7 |
Cytoplasm | 122 | 34 | 98 | 151 | 131 | 262 | 362 | 543 | 262 | 286 | 220 | 298 | 121 | 222 | 255 | 263 | 94 | 183 | 20 | 19 | 23 | 16 | 38 | 36 |
Endoplasmic Reticulum | 141 | 13 | 20 | 19 | 45 | 15 | 28 | 15 | 5 | 12 | 2 | 7 | 22 | 30 | 16 | 15 | 8 | 12 | 247 | 38 | 103 | 97 | 136 | 212 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 1 | 3 | 2 | 0 | 1 | 1 | 0 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 3 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 0 | 1 | 1 | 7 | 1 | 4 | 9 | 5 | 12 | 12 | 8 | 19 | 0 | 1 | 3 | 3 | 4 | 4 | 2 | 0 | 7 | 1 | 3 | 2 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 2 | 5 | 1 | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 5 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 8 | 2 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 6 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 2 | 0 | 1 | 2 | 0 | 1 | 0 | 23 | 14 | 11 | 13 | 11 | 11 | 15 | 26 | 34 |
Unique Cell Count | 267 | 46 | 133 | 190 | 203 | 300 | 426 | 598 | 296 | 327 | 244 | 330 | 153 | 275 | 289 | 325 | 131 | 222 | 301 | 82 | 164 | 146 | 231 | 315 |
Labelled Cell Count | 299 | 51 | 146 | 223 | 237 | 376 | 541 | 695 | 354 | 406 | 296 | 408 | 201 | 333 | 372 | 367 | 151 | 266 | 301 | 82 | 164 | 146 | 231 | 315 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.6 | 6.7 | 5.8 | 5.8 | 6.4 | 6.1 | 5.6 | 5.4 | 5.3 | 5.1 | 5.1 | 4.9 | 7.5 | 6.9 | 5.8 | 24.5 | 20.3 | 22.9 | 7.8 | 7.7 | 8.1 |
Std Deviation (1e-4) | 1.0 | 1.0 | 0.7 | 0.8 | 0.8 | 1.0 | 1.0 | 1.0 | 1.1 | 1.0 | 1.0 | 1.1 | 1.3 | 1.0 | 1.0 | 33.9 | 30.2 | 33.8 | 1.7 | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.14 | 0.09 | -0.05 | -0.09 | -0.13 | -0.18 | -0.18 | -0.23 | 0.37 | 0.25 | 0.01 | 2.08 | 1.82 | 1.99 | 0.43 | 0.42 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 2.1 | 2.5 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 2.2 | 0 |
Bud | 0.5 | 0.4 | 1.2 | 1.5 | 1.3 | 0.9 | 0.2 | 0.5 | 0.4 | -1.4 | 0 | -1.0 | 0 | -1.4 | -0.9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.4 | 0.9 | 0.4 | 0.6 | -1.5 | 0 | 1.4 | 0.8 | 1.0 | 3.6 | 3.2 | 3.5 | -2.2 | -2.0 | -1.0 |
Cytoplasm | 1.2 | -1.8 | 3.5 | 3.0 | 5.4 | 3.9 | 3.6 | 4.2 | 4.6 | 1.1 | 1.6 | 3.8 | 1.7 | -0.4 | 2.0 |
Endoplasmic Reticulum | -1.4 | 1.6 | -3.5 | -3.0 | -6.1 | -5.5 | -4.3 | -5.6 | -5.4 | -0.2 | -1.2 | -3.2 | -3.8 | -2.4 | -3.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 3.5 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.0789 | 3.6773 | 3.5974 | 3.4402 | 3.2945 | 3.9342 | 2.9049 | 3.6387 | 3.4222 | 3.2342 | 4.0449 | 3.7013 | 2.7022 | 4.9026 | 3.2932 | 3.2325 | 3.6747 | 3.5968 |
Actin | 0.0006 | 0.001 | 0.0014 | 0.0026 | 0.0004 | 0.0006 | 0.0339 | 0.0037 | 0.0121 | 0.0079 | 0.0476 | 0.0027 | 0.0006 | 0.0016 | 0.0019 | 0.001 | 0.0003 | 0.0008 |
Bud | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0003 | 0.0005 | 0.0003 | 0.0006 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0005 | 0.0005 | 0.0013 | 0.0026 | 0.0018 | 0.0034 | 0.0022 | 0.0049 | 0.0017 | 0.0028 | 0.0087 | 0.0052 | 0.0001 | 0.0004 | 0.0023 | 0.0006 | 0.0018 | 0.0025 |
Bud Periphery | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0006 | 0.0006 | 0.0006 | 0.0006 | 0.0011 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0008 | 0.0003 |
Bud Site | 0.0006 | 0.0023 | 0.0018 | 0.0005 | 0.0004 | 0.0001 | 0.0031 | 0.0094 | 0.0076 | 0.001 | 0.0009 | 0.0003 | 0.0003 | 0.0048 | 0.009 | 0.0004 | 0.0007 | 0.0002 |
Cell Periphery | 0.0085 | 0.0136 | 0.0048 | 0.0055 | 0.0026 | 0.0033 | 0.0073 | 0.0176 | 0.0048 | 0.0061 | 0.0035 | 0.0051 | 0.0055 | 0.0117 | 0.0085 | 0.0053 | 0.0236 | 0.0046 |
Cytoplasm | 0.6783 | 0.6831 | 0.7387 | 0.6745 | 0.8123 | 0.709 | 0.3602 | 0.571 | 0.5936 | 0.568 | 0.4391 | 0.5624 | 0.4742 | 0.6044 | 0.5016 | 0.5884 | 0.7639 | 0.6373 |
Cytoplasmic Foci | 0.0059 | 0.0115 | 0.012 | 0.008 | 0.006 | 0.005 | 0.0339 | 0.0163 | 0.0176 | 0.044 | 0.028 | 0.0122 | 0.0114 | 0.014 | 0.0145 | 0.0045 | 0.008 | 0.0184 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0006 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.1824 | 0.1857 | 0.1275 | 0.1983 | 0.0692 | 0.1796 | 0.271 | 0.1721 | 0.2092 | 0.1145 | 0.1697 | 0.2104 | 0.3241 | 0.118 | 0.2405 | 0.0743 | 0.1348 | 0.1789 |
Endosome | 0.0039 | 0.0073 | 0.014 | 0.011 | 0.0187 | 0.0082 | 0.0717 | 0.0251 | 0.0363 | 0.0581 | 0.14 | 0.0291 | 0.0085 | 0.0175 | 0.0329 | 0.0048 | 0.0105 | 0.0119 |
Golgi | 0.0007 | 0.001 | 0.0008 | 0.0025 | 0.0004 | 0.0006 | 0.0126 | 0.0025 | 0.007 | 0.0164 | 0.048 | 0.0083 | 0.002 | 0.0018 | 0.0054 | 0.0009 | 0.0006 | 0.0008 |
Lipid Particles | 0.0152 | 0.0368 | 0.0169 | 0.0108 | 0.0042 | 0.0044 | 0.0554 | 0.053 | 0.0138 | 0.0253 | 0.0294 | 0.0153 | 0.0463 | 0.0275 | 0.0326 | 0.004 | 0.008 | 0.0165 |
Mitochondria | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0066 | 0.0021 | 0.007 | 0.0077 | 0.015 | 0.005 | 0.0001 | 0.0001 | 0.0005 | 0.0006 | 0.0001 | 0.0002 |
None | 0.0915 | 0.0396 | 0.0479 | 0.0061 | 0.0242 | 0.0439 | 0.0739 | 0.0529 | 0.0377 | 0.0468 | 0.0094 | 0.0375 | 0.1055 | 0.1333 | 0.0715 | 0.2818 | 0.0102 | 0.1024 |
Nuclear Periphery | 0.0031 | 0.003 | 0.0035 | 0.0029 | 0.0045 | 0.0032 | 0.017 | 0.0046 | 0.0049 | 0.0056 | 0.0159 | 0.0147 | 0.0077 | 0.0027 | 0.0053 | 0.002 | 0.002 | 0.002 |
Nucleolus | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0015 | 0.0002 | 0.0018 | 0.0008 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.001 | 0.0011 | 0.0015 | 0.0016 | 0.0057 | 0.0019 | 0.003 | 0.0029 | 0.0018 | 0.0032 | 0.0058 | 0.0042 | 0.0024 | 0.0014 | 0.0014 | 0.0006 | 0.0008 | 0.001 |
Peroxisomes | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.008 | 0.0007 | 0.0021 | 0.0087 | 0.0015 | 0.0009 | 0.0001 | 0.0001 | 0.0141 | 0.0001 | 0 | 0.0001 |
Punctate Nuclear | 0.0005 | 0.0007 | 0.0008 | 0.0004 | 0.0006 | 0.0004 | 0.0037 | 0.0019 | 0.0008 | 0.0094 | 0.0076 | 0.0013 | 0.0004 | 0.001 | 0.0006 | 0.0002 | 0.0002 | 0.0004 |
Vacuole | 0.0068 | 0.0122 | 0.0259 | 0.0708 | 0.0472 | 0.0356 | 0.032 | 0.0561 | 0.0396 | 0.0694 | 0.0249 | 0.0835 | 0.0103 | 0.0585 | 0.0557 | 0.0287 | 0.0329 | 0.021 |
Vacuole Periphery | 0.0001 | 0.0001 | 0.0005 | 0.0012 | 0.0012 | 0.0004 | 0.0023 | 0.0006 | 0.0009 | 0.0021 | 0.0023 | 0.0015 | 0.0002 | 0.0006 | 0.001 | 0.0005 | 0.0004 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 30.2984 | 21.8296 | 33.597 | 33.8292 | 25.6025 | 21.9956 | 34.1979 | 35.5874 | 34.9926 | 36.2714 |
Translational Efficiency | 0.4024 | 0.4522 | 0.3802 | 0.3696 | 0.3993 | 0.5183 | 0.3305 | 0.3437 | 0.3628 | 0.3356 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1165 | 41 | 750 | 1390 | 1749 | 1194 | 239 | 216 | 2914 | 1235 | 989 | 1606 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 763.91 | 312.80 | 1215.26 | 967.86 | 848.39 | 860.88 | 1144.30 | 1051.27 | 814.62 | 842.68 | 1198.11 | 979.08 |
Standard Deviation | 119.75 | 13.02 | 137.95 | 138.92 | 112.86 | 119.30 | 116.69 | 137.74 | 122.85 | 152.99 | 136.55 | 141.65 |
Intensity Change Log 2 | — | -1.288162 | 0.669790 | 0.341396 | — | 0.021085 | 0.431666 | 0.309334 | — | -0.458081 | 0.549398 | 0.324614 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001130 | 0.000039 | 0.000553 | 0.017893 | 0.001230 | 0.001763 | 0.010309 | 0.029221 | 0.001190 | 0.001706 | 0.002910 | 0.019417 |
Bud Neck | 0.014172 | 0.000006 | 0.006472 | 0.021983 | 0.011641 | 0.017013 | 0.008359 | 0.025622 | 0.012653 | 0.016448 | 0.006928 | 0.022472 |
Bud Site | 0.006617 | 0.000013 | 0.020637 | 0.059233 | 0.007491 | 0.011463 | 0.020724 | 0.069701 | 0.007142 | 0.011083 | 0.020658 | 0.060641 |
Cell Periphery | 0.010375 | 0.000049 | 0.001042 | 0.007970 | 0.007054 | 0.008242 | 0.020918 | 0.024455 | 0.008382 | 0.007970 | 0.005845 | 0.010187 |
Cytoplasm | 0.526089* | 0.000029 | 0.608416* | 0.333322* | 0.392300* | 0.396488* | 0.201442* | 0.276716* | 0.445788* | 0.383326* | 0.510068* | 0.325708* |
Cytoplasmic Foci | 0.086802 | 0.000017 | 0.029125 | 0.073535 | 0.056395 | 0.062249 | 0.059313 | 0.072900 | 0.068551 | 0.060183 | 0.036420 | 0.073450 |
Eisosomes | 0.012567 | 0.000053 | 0.000181 | 0.007137 | 0.005187 | 0.006472 | 0.006710 | 0.015980 | 0.008138 | 0.006259 | 0.001759 | 0.008327 |
Endoplasmic Reticulum | 0.132922* | 0.000001 | 0.019111 | 0.040578 | 0.352860* | 0.281349* | 0.263831* | 0.079456 | 0.264930* | 0.272008* | 0.078250 | 0.045807 |
Endosome | 0.011418 | 0.000001 | 0.003502 | 0.017584 | 0.011023 | 0.018894 | 0.012040 | 0.010029 | 0.011181 | 0.018267 | 0.005565 | 0.016568 |
Golgi | 0.022905 | 0.000005 | 0.000361 | 0.004641 | 0.027083 | 0.032426 | 0.002173 | 0.001879 | 0.025413 | 0.031349 | 0.000799 | 0.004269 |
Lipid Particles | 0.052887 | 0.000021 | 0.007742 | 0.022627 | 0.040450 | 0.049576 | 0.031212 | 0.029749 | 0.045422 | 0.047931 | 0.013414 | 0.023585 |
Mitochondria | 0.003018 | 0.000010 | 0.000292 | 0.003947 | 0.004736 | 0.006593 | 0.000088 | 0.000524 | 0.004049 | 0.006374 | 0.000243 | 0.003487 |
Mitotic Spindle | 0.000062 | 0.000338 | 0.000105 | 0.010391 | 0.000247 | 0.000401 | 0.004082 | 0.000210 | 0.000173 | 0.000399 | 0.001066 | 0.009022 |
None | 0.009832 | 0.999270* | 0.001204 | 0.001839 | 0.004529 | 0.002601 | 0.000106 | 0.001519 | 0.006649 | 0.035688 | 0.000939 | 0.001796 |
Nuclear Periphery | 0.000938 | 0.000000 | 0.000572 | 0.000769 | 0.000408 | 0.000491 | 0.002388 | 0.000357 | 0.000620 | 0.000475 | 0.001011 | 0.000713 |
Nuclear Periphery Foci | 0.004463 | 0.000026 | 0.012117 | 0.008502 | 0.002761 | 0.003176 | 0.039564 | 0.017011 | 0.003442 | 0.003071 | 0.018750 | 0.009646 |
Nucleolus | 0.001101 | 0.000024 | 0.000415 | 0.000430 | 0.000383 | 0.000935 | 0.000108 | 0.000121 | 0.000670 | 0.000905 | 0.000341 | 0.000388 |
Nucleus | 0.052449 | 0.000009 | 0.091543 | 0.054291 | 0.021734 | 0.021598 | 0.060218 | 0.034256 | 0.034013 | 0.020882 | 0.083973 | 0.051596 |
Peroxisomes | 0.005805 | 0.000089 | 0.000344 | 0.004339 | 0.005089 | 0.006526 | 0.000835 | 0.003040 | 0.005375 | 0.006312 | 0.000463 | 0.004165 |
Vacuole | 0.043731 | 0.000001 | 0.196083 | 0.306711* | 0.045684 | 0.069245 | 0.255414* | 0.307096* | 0.044903 | 0.066946 | 0.210421* | 0.306763* |
Vacuole Periphery | 0.000715 | 0.000000 | 0.000182 | 0.002278 | 0.001715 | 0.002500 | 0.000166 | 0.000160 | 0.001315 | 0.002417 | 0.000178 | 0.001993 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 7.42 | 3.59 | -9.78 | -10.51 | -10.17 | -2.29 | -4.04 | -5.38 | -5.26 | -3.24 | -2.36 | -3.03 | -11.11 | -10.68 | -9.43 |
Bud Neck | 12.75 | 6.40 | -3.32 | -14.36 | -9.20 | -2.82 | 2.30 | -3.27 | -1.77 | -4.25 | -2.25 | 6.26 | -5.17 | -2.12 | -10.01 |
Bud Site | 9.12 | -4.51 | -13.68 | -15.87 | -8.28 | -2.77 | -4.96 | -7.54 | -6.96 | -5.57 | -2.96 | -5.69 | -15.38 | -13.33 | -9.76 |
Cell Periphery | 13.43 | 12.05 | 3.35 | -11.65 | -9.90 | -1.42 | -3.26 | -3.70 | -3.39 | -0.36 | 0.53 | 2.64 | -0.80 | -1.13 | -2.87 |
Cytoplasm | 80.88* | -7.04 | 21.55 | -47.96* | 26.99 | -0.44 | 13.79 | 7.62 | 7.68 | -3.44 | 7.48 | -5.67 | 16.82 | 7.17 | 17.59 |
Cytoplasmic Foci | 24.90 | 14.04 | 3.75 | -26.09 | -11.89 | -1.67 | -0.57 | -2.45 | -1.58 | -1.63 | 2.60 | 10.98 | -0.72 | -2.98 | -10.29 |
Eisosomes | 11.37 | 11.27 | 4.81 | -8.84 | -8.68 | -2.32 | -0.80 | -3.02 | -2.59 | -2.37 | 2.93 | 10.51 | 0.88 | -1.31 | -6.94 |
Endoplasmic Reticulum | 21.34* | 17.55 | 13.98 | -12.34 | -5.26 | 6.15 | 4.96 | 21.11 | 15.19 | 8.26 | -0.70 | 23.54 | 34.91 | 25.80 | 5.01 |
Endosome | 14.66 | 9.76 | -3.21 | -13.20 | -10.15 | -6.16 | -1.17 | 0.96 | 4.70 | 1.49 | -5.75 | 11.09 | -3.46 | 1.93 | -8.61 |
Golgi | 18.74* | 18.45 | 13.42 | -5.62 | -5.14 | -3.10 | 23.48 | 23.94 | 21.97 | 1.06 | -3.82 | 31.06 | 21.79 | 18.77 | -4.72 |
Lipid Particles | 20.38* | 16.72 | 11.02 | -18.05 | -9.79 | -3.70 | 3.30 | 3.73 | 6.16 | 0.66 | -1.06 | 19.50 | 12.97 | 11.72 | -6.35 |
Mitochondria | 5.68 | 5.20 | -0.39 | -4.66 | -4.31 | -1.76 | 9.67 | 7.23 | 6.07 | -1.10 | -2.37 | 10.69 | 1.49 | 3.07 | -4.41 |
Mitotic Spindle | -5.08 | -2.22 | -5.06 | -4.91 | -5.00 | -0.52 | -0.97 | -0.93 | -0.74 | 0.74 | -0.90 | -0.98 | -5.04 | -4.87 | -3.92 |
None | -616.11* | 5.46 | 4.74 | 1443.81* | -0.77 | 2.11 | 6.55 | 2.73 | 1.10 | -1.36 | -5.61 | 7.57 | 5.24 | 6.62 | -1.21 |
Nuclear Periphery | 6.75 | 2.40 | 0.87 | -6.38 | -1.47 | -1.02 | -3.90 | 1.04 | 1.59 | 4.00 | 1.55 | -2.81 | -0.83 | -1.91 | 1.81 |
Nuclear Periphery Foci | 8.68 | -7.17 | -4.41 | -11.45 | 3.62 | -1.09 | -10.66 | -8.55 | -8.20 | 6.03 | 0.87 | -12.81 | -8.42 | -8.63 | 7.13 |
Nucleolus | 3.83 | 2.56 | 2.50 | -5.92 | -0.06 | -1.91 | 9.60 | 9.60 | 2.80 | -0.44 | -0.75 | 3.04 | 2.52 | 1.92 | -0.62 |
Nucleus | 27.25 | -10.79 | -0.82 | -25.52 | 9.78 | 0.06 | -6.71 | -3.50 | -3.41 | 4.12 | 8.24 | -17.31 | -8.34 | -13.57 | 9.58 |
Peroxisomes | 15.93 | 15.17 | 3.14 | -7.81 | -7.30 | -3.13 | 14.88 | 3.94 | 5.92 | -3.95 | -2.24 | 22.14 | 3.36 | 4.56 | -7.71 |
Vacuole | 22.55 | -25.25 | -38.97 | -46.35 | -13.21 | -6.17 | -14.90 | -16.55 | -15.05 | -2.88 | -6.36 | -28.97 | -42.53 | -36.40 | -12.46 |
Vacuole Periphery | 15.09 | 11.02 | -3.23 | -5.13 | -4.69 | -1.76 | 4.55 | 4.56 | 8.05 | 0.27 | -3.19 | 5.58 | -1.02 | 1.64 | -4.68 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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