Standard name
Human Ortholog
Description Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD; required for ubiquitination of a subset of cytosolic misfolded proteins upon heat shock
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.09 0.34 0.16 0.17 0.25 0.21 0.16 0.15 0.16 0.15 0.17 0.13 0.2 0.2 0.22 0.22 0.23 0.09 0.05 0.17 0.07 0.06 0.08
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0.18 0.13 0.46 0.42 0.53 0.32 0.06 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.53 0.54 0.53 0.67 0.59 0.52 0.57 0.59 0.51 0.44 0.46 0.42 0.57 0.54 0.5 0.43 0.5 0.49 0.61 0.61 0.4 0.41 0.46 0.41
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.33 0.49 0.13 0.14 0.27 0.23 0.18 0.24 0.17 0.22 0.17 0.25 0.32 0.3 0.36 0.39 0.31 0.3 0.15 0.19 0.26 0.29 0.26 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 4 1
Bud 1 0 0 1 1 0 2 1 3 6 7 5 0 0 0 0 0 0 4 11 5 13 19 19
Bud Neck 1 0 1 2 0 1 2 1 0 1 1 2 0 0 0 0 0 1 1 2 1 2 4 7
Bud Site 0 0 0 0 0 0 2 2 5 4 7 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0
Cytoplasm 40 39 77 41 59 67 68 56 58 74 59 63 41 57 66 105 88 70 21 23 57 11 23 28
Endoplasmic Reticulum 1 0 4 0 2 1 1 0 1 1 1 0 1 1 0 29 23 13 1 0 2 1 3 6
Endosome 1 8 2 2 6 2 7 4 1 3 2 9 1 4 9 19 14 13 7 12 5 1 7 8
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 2 0 1 0 2
Mitochondria 3 13 6 10 12 38 59 47 176 196 203 118 18 2 2 9 7 9 2 5 13 4 14 7
Nucleus 107 222 121 167 206 138 186 210 193 208 177 158 182 159 166 204 198 150 146 290 136 72 187 141
Nuclear Periphery 1 0 0 0 0 0 1 0 0 3 1 1 1 0 0 1 0 0 3 3 1 1 3 4
Nucleolus 0 1 0 0 1 0 0 0 0 1 0 0 0 1 2 1 0 0 3 13 2 1 2 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 1 0 2
SpindlePole 0 3 0 2 2 0 1 5 0 1 1 2 0 1 0 2 3 0 6 14 13 4 15 14
Vac/Vac Membrane 68 203 29 35 95 60 58 86 63 104 65 94 101 87 120 186 124 92 35 90 87 50 105 88
Unique Cell Count 203 411 227 250 347 266 328 356 381 470 382 372 317 292 332 479 398 306 241 476 341 175 404 347
Labelled Cell Count 223 490 241 261 384 307 387 412 500 602 524 462 345 312 368 560 458 349 241 476 341 175 404 347


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.4 5.5 5.5 6.1 5.0 5.1 5.3 4.5 4.5 4.2 5.0 5.7 5.9 5.7 7.4 7.6 7.5 5.0 5.0 5.6
Std Deviation (1e-4) 0.8 0.6 1.1 1.2 1.1 1.3 1.3 0.8 1.1 1.1 1.0 1.4 1.5 1.5 1.4 1.5 1.7 1.7 1.3 1.7 2.0
Intensity Change (Log2) – – – -0.01 0.14 -0.13 -0.1 -0.07 -0.29 -0.28 -0.4 -0.15 0.05 0.1 0.06 0.43 0.47 0.44 -0.15 -0.12 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -4.4 -4.7 -2.1 -3.5 -5.1 -5.4 -5.5 -5.3 -4.8 -5.9 -3.7 -3.7 -3.4 -3.2 -2.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.5 2.4 1.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 2.3 2.0 2.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.8 0.5 4.5 5.5 4.3 0 0 0 8.5 1.7 0 0 0 0 0.2
Nucleus 3.0 1.4 -0.3 0.8 1.4 -0.6 -2.2 -1.7 -2.6 1.0 0.3 -0.8 -2.7 -0.9 -1.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.4 4.2 2.8 1.6 3.4 1.3 2.9 1.4 3.7 5.1 4.6 6.1 7.0 5.1 4.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8786 3.7778 3.2323 2.5679 2.77 2.973 2.2818 2.337 1.9427 2.0103 1.8765 1.8414 1.5355 1.9365 1.7369 1.4674 1.9115 1.7668
Actin 0.036 0.0113 0.0076 0.0245 0.0072 0.0047 0.0193 0.0002 0.0023 0.0185 0.0107 0.0033 0.003 0.0001 0.0023 0 0.0002 0
Bud 0.0005 0.0003 0.0002 0.0001 0.0004 0.0002 0.0005 0.0002 0.0047 0.0029 0.0042 0.0004 0.0001 0.0003 0.0001 0 0 0
Bud Neck 0.0068 0.0004 0.0072 0.0003 0.001 0.0054 0.0027 0.0008 0.0009 0.0029 0.001 0.0041 0.0009 0.0003 0.0004 0.0003 0.0009 0.0039
Bud Periphery 0.0009 0.0002 0.0004 0.0001 0.0026 0.0002 0.0009 0.0004 0.0011 0.0048 0.0042 0.0016 0.0001 0.0001 0.0001 0 0 0
Bud Site 0.0034 0.0043 0.0036 0.0001 0.0005 0.0009 0.0067 0.0034 0.0013 0.0102 0.0101 0.0005 0.0003 0.0003 0.0004 0 0.0001 0
Cell Periphery 0.0007 0 0.0004 0 0.0003 0 0.0004 0.0004 0.0001 0.0005 0.0002 0.0002 0 0 0 0 0 0
Cytoplasm 0.0148 0.0093 0.0214 0.0399 0.0052 0.0054 0.0063 0.0066 0.0037 0.0049 0.014 0.0037 0.0063 0.0111 0.0033 0.0007 0.0014 0.001
Cytoplasmic Foci 0.0198 0.0098 0.0048 0.0076 0.0029 0.0054 0.0461 0.0003 0.005 0.007 0.0126 0.0012 0.0074 0.0002 0.0048 0 0 0.0001
Eisosomes 0.0008 0.0001 0.0004 0.0002 0.0013 0 0.0001 0 0 0.0003 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.009 0.0003 0.0014 0.002 0.0005 0.0005 0.0055 0.0005 0.0007 0.0029 0.0009 0.001 0.0005 0.0003 0.0011 0 0.0002 0
Endosome 0.0409 0.0019 0.0026 0.0064 0.0011 0.0027 0.0506 0.0018 0.0069 0.022 0.0107 0.0042 0.003 0.0002 0.0055 0 0.0003 0.0001
Golgi 0.012 0.0025 0.0015 0.0166 0.0007 0.0037 0.0195 0.0001 0.0022 0.019 0.0067 0.0025 0.0008 0 0.0011 0 0 0
Lipid Particles 0.0438 0.003 0.0033 0.0018 0.0011 0.0043 0.0296 0.0008 0.0058 0.0139 0.0016 0.0036 0.0022 0 0.0021 0 0 0
Mitochondria 0.0128 0.002 0.0169 0.0006 0.0504 0.0041 0.0047 0.0014 0.0077 0.0264 0.0053 0.0103 0.0002 0.0002 0.0003 0.0001 0.0003 0.0001
None 0.0237 0.0284 0.0309 0.1049 0.1932 0.0278 0.0025 0.0012 0.001 0.0117 0.0008 0.0009 0.0116 0.0068 0.0017 0.0002 0.0005 0.0001
Nuclear Periphery 0.0175 0.0091 0.011 0.0082 0.0079 0.0054 0.0447 0.0268 0.0192 0.0164 0.0155 0.0128 0.0111 0.0143 0.0144 0.0021 0.0323 0.002
Nucleolus 0.0099 0.0082 0.006 0.0051 0.007 0.0164 0.0068 0.0063 0.0033 0.0029 0.0168 0.0125 0.0069 0.0065 0.006 0.0047 0.0109 0.0118
Nucleus 0.6882 0.8861 0.8542 0.7756 0.708 0.8963 0.6966 0.9425 0.904 0.7741 0.8556 0.9139 0.7783 0.8885 0.8135 0.9214 0.8435 0.876
Peroxisomes 0.0098 0.0082 0.0116 0.0009 0.0007 0.0074 0.0239 0.0001 0.0158 0.0154 0.0157 0.0006 0.0036 0 0.0011 0 0 0
Punctate Nuclear 0.0408 0.0143 0.0134 0.0046 0.0059 0.0085 0.0241 0.0048 0.0106 0.0357 0.0063 0.0208 0.1631 0.0696 0.1412 0.0703 0.1089 0.1046
Vacuole 0.0058 0.0002 0.0011 0.0002 0.0009 0.0006 0.0063 0.0008 0.0029 0.0036 0.0056 0.0014 0.0003 0.0012 0.0003 0 0.0003 0.0001
Vacuole Periphery 0.0022 0.0001 0.0002 0.0001 0.001 0.0002 0.0021 0.0005 0.0008 0.0041 0.0017 0.0006 0.0002 0.0002 0.0002 0 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.6709 7.5792 10.1769 15.4881 10.2074 11.7018 14.5968 18.2259 17.1633 13.3509
Translational Efficiency 0.9807 0.904 0.9191 0.7684 0.6946 0.8118 0.6399 0.5639 0.6557 0.6536

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2330 1567 1089 1553 1620 1157 592 983 3950 2724 1681 2536

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 666.44 729.56 850.37 809.39 705.74 741.31 797.77 771.95 682.56 734.55 831.85 794.88
Standard Deviation 74.60 118.06 201.52 116.21 92.62 101.14 130.34 101.73 84.70 111.34 181.45 112.31
Intensity Change Log 2 — 0.130552 0.351616 0.280360 — 0.070940 0.176836 0.129371 — 0.100200 0.264369 0.204678

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000047 0.000230 0.000751 0.000627 0.000085 0.000166 0.000187 0.000878 0.000063 0.000203 0.000552 0.000724
Bud Neck 0.003099 0.004319 0.006622 0.014856 0.001685 0.004828 0.001346 0.005153 0.002519 0.004535 0.004764 0.011095
Bud Site 0.001212 0.001134 0.005087 0.019511 0.001780 0.002308 0.000413 0.014713 0.001445 0.001633 0.003441 0.017651
Cell Periphery 0.000102 0.000093 0.000211 0.000202 0.000206 0.000116 0.000820 0.000227 0.000145 0.000103 0.000426 0.000212
Cytoplasm 0.272396* 0.158642* 0.192215* 0.176244* 0.141599* 0.163368* 0.104323* 0.196531* 0.218753* 0.160649* 0.161262* 0.184108*
Cytoplasmic Foci 0.022950 0.013116 0.003597 0.005319 0.014245 0.019850 0.009330 0.004181 0.019380 0.015976 0.005616 0.004878
Eisosomes 0.000024 0.000026 0.000034 0.000031 0.000024 0.000023 0.000200 0.000036 0.000024 0.000025 0.000092 0.000033
Endoplasmic Reticulum 0.000732 0.004094 0.006639 0.005282 0.002344 0.002073 0.003057 0.004530 0.001393 0.003235 0.005378 0.004990
Endosome 0.001026 0.001451 0.001430 0.006131 0.000687 0.001411 0.000500 0.001577 0.000887 0.001434 0.001103 0.004366
Golgi 0.000347 0.000618 0.000126 0.007120 0.000103 0.000394 0.000037 0.000305 0.000247 0.000523 0.000095 0.004478
Lipid Particles 0.002115 0.002546 0.001011 0.001007 0.002653 0.002180 0.001171 0.001152 0.002336 0.002391 0.001068 0.001064
Mitochondria 0.003292 0.004128 0.001060 0.004533 0.002944 0.002698 0.000352 0.000962 0.003149 0.003520 0.000810 0.003149
Mitotic Spindle 0.002090 0.002960 0.009563 0.043651 0.001327 0.006117 0.000255 0.006128 0.001777 0.004301 0.006285 0.029106
None 0.008631 0.014699 0.036369 0.006446 0.010629 0.008605 0.031072 0.009358 0.009450 0.012110 0.034504 0.007575
Nuclear Periphery 0.001857 0.004256 0.002535 0.003650 0.002868 0.003523 0.001043 0.002288 0.002271 0.003945 0.002009 0.003122
Nuclear Periphery Foci 0.000369 0.001619 0.002798 0.001205 0.000905 0.001130 0.000315 0.001121 0.000589 0.001411 0.001924 0.001172
Nucleolus 0.002199 0.005703 0.003092 0.001713 0.002814 0.004010 0.001758 0.001197 0.002451 0.004984 0.002622 0.001513
Nucleus 0.671829* 0.772312* 0.705020* 0.660753* 0.808811* 0.768958* 0.830903* 0.720802* 0.728009* 0.770888* 0.749352* 0.684029*
Peroxisomes 0.001604 0.001236 0.000340 0.000471 0.000649 0.002116 0.000213 0.000442 0.001212 0.001610 0.000296 0.000459
Vacuole 0.003776 0.006091 0.021281 0.037538 0.003418 0.005764 0.012641 0.027977 0.003629 0.005952 0.018238 0.033832
Vacuole Periphery 0.000303 0.000724 0.000219 0.003709 0.000224 0.000364 0.000064 0.000443 0.000271 0.000571 0.000164 0.002443

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.10 -7.73 -10.48 -6.19 2.39 -2.04 -5.54 -2.55 -2.17 -1.61 -6.16 -8.68 -5.59 -4.05 0.21
Bud Neck -1.73 -6.82 -8.09 -6.61 -2.25 -3.46 -1.30 -5.03 -0.76 -4.59 -3.49 -7.08 -9.38 -6.17 -3.17
Bud Site -0.06 -5.81 -7.45 -7.50 -4.02 -0.68 1.78 -4.08 -3.86 -4.68 -0.63 -4.76 -8.33 -8.20 -5.81
Cell Periphery 0.75 -6.08 -5.40 -6.10 1.55 2.24 -3.55 0.26 -2.72 3.77 2.33 -4.52 -1.49 -5.28 3.96
Cytoplasm 15.76 14.92 17.90 1.00 -0.38 -2.60 7.75 -3.28 -0.70 -9.66 10.93 15.40 11.62 0.37 -5.24
Cytoplasmic Foci 5.59 16.19 15.49 7.52 -1.18 -2.61 4.84 8.24 8.04 2.88 2.20 15.95 17.39 11.01 1.27
Eisosomes -2.38 -8.27 -10.47 -6.98 0.55 0.23 -7.84 -9.31 -9.19 6.25 -1.76 -9.60 -13.99 -11.37 5.97
Endoplasmic Reticulum -13.94 -16.18 -23.99 -8.17 4.86 0.75 -6.66 -11.15 -11.90 -1.74 -10.84 -17.05 -24.89 -13.83 3.64
Endosome -2.06 -4.79 -4.74 -3.71 -3.04 -3.32 -0.91 -6.93 -0.81 -5.99 -3.70 -5.14 -6.06 -3.74 -4.10
Golgi -1.04 2.06 -2.66 -2.36 -2.91 -2.10 5.00 -0.94 1.14 -1.49 -1.79 2.35 -2.73 -2.22 -3.00
Lipid Particles -1.67 4.02 4.90 5.89 0.29 1.19 4.32 4.43 3.66 -0.18 -0.40 5.89 6.58 6.93 0.10
Mitochondria -0.76 2.67 -0.20 0.72 -5.03 0.18 3.15 2.45 3.59 -5.76 -0.53 3.93 1.10 1.87 -5.63
Mitotic Spindle -0.63 -4.70 -7.77 -7.41 -3.35 -2.45 1.20 -2.79 -0.26 -3.37 -2.32 -4.49 -8.23 -6.51 -3.93
None -3.78 -4.77 4.23 6.47 6.10 1.15 -3.03 1.57 0.46 3.76 -2.27 -5.57 3.84 5.60 7.08
Nuclear Periphery -5.85 -7.83 -13.96 -1.77 -2.71 -1.48 4.47 -1.80 0.43 -8.14 -5.58 -5.02 -12.12 -1.40 -4.78
Nuclear Periphery Foci -5.05 -6.02 -4.80 2.21 4.19 -0.22 2.43 -1.62 -0.85 -6.14 -3.57 -4.91 -4.42 0.91 2.79
Nucleolus -5.36 -1.40 2.36 7.05 4.15 -2.09 3.38 4.85 4.94 1.86 -5.45 0.51 4.69 8.54 4.63
Nucleus -11.47 -5.74 -1.91 8.37 3.70 4.06 -3.31 8.81 4.44 10.42 -6.38 -5.51 3.98 9.33 8.27
Peroxisomes 0.77 3.27 3.35 4.55 0.43 -2.76 0.77 1.51 3.19 0.09 -1.28 3.36 3.65 4.95 0.24
Vacuole -4.86 -12.88 -19.03 -18.03 -7.01 -2.69 -6.34 -14.00 -13.28 -6.44 -5.29 -14.12 -23.62 -22.38 -9.37
Vacuole Periphery -1.92 0.96 -3.10 -2.00 -3.27 -2.30 4.43 -0.99 0.52 -2.70 -2.35 2.13 -3.21 -1.97 -3.55
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD; required for ubiquitination of a subset of cytosolic misfolded proteins upon heat shock
Localization
Cell Percentages nucleus (70%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Hul5

Hul5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hul5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available