Standard name | |
---|---|
Human Ortholog | |
Description | Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD; required for ubiquitination of a subset of cytosolic misfolded proteins upon heat shock |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 4 | 1 |
Bud | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 3 | 6 | 7 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 11 | 5 | 13 | 19 | 19 |
Bud Neck | 1 | 0 | 1 | 2 | 0 | 1 | 2 | 1 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 2 | 4 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 5 | 4 | 7 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 40 | 39 | 77 | 41 | 59 | 67 | 68 | 56 | 58 | 74 | 59 | 63 | 41 | 57 | 66 | 105 | 88 | 70 | 21 | 23 | 57 | 11 | 23 | 28 |
Endoplasmic Reticulum | 1 | 0 | 4 | 0 | 2 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 29 | 23 | 13 | 1 | 0 | 2 | 1 | 3 | 6 |
Endosome | 1 | 8 | 2 | 2 | 6 | 2 | 7 | 4 | 1 | 3 | 2 | 9 | 1 | 4 | 9 | 19 | 14 | 13 | 7 | 12 | 5 | 1 | 7 | 8 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 2 |
Mitochondria | 3 | 13 | 6 | 10 | 12 | 38 | 59 | 47 | 176 | 196 | 203 | 118 | 18 | 2 | 2 | 9 | 7 | 9 | 2 | 5 | 13 | 4 | 14 | 7 |
Nucleus | 107 | 222 | 121 | 167 | 206 | 138 | 186 | 210 | 193 | 208 | 177 | 158 | 182 | 159 | 166 | 204 | 198 | 150 | 146 | 290 | 136 | 72 | 187 | 141 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 3 | 3 | 1 | 1 | 3 | 4 |
Nucleolus | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 3 | 13 | 2 | 1 | 2 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 |
SpindlePole | 0 | 3 | 0 | 2 | 2 | 0 | 1 | 5 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 2 | 3 | 0 | 6 | 14 | 13 | 4 | 15 | 14 |
Vac/Vac Membrane | 68 | 203 | 29 | 35 | 95 | 60 | 58 | 86 | 63 | 104 | 65 | 94 | 101 | 87 | 120 | 186 | 124 | 92 | 35 | 90 | 87 | 50 | 105 | 88 |
Unique Cell Count | 203 | 411 | 227 | 250 | 347 | 266 | 328 | 356 | 381 | 470 | 382 | 372 | 317 | 292 | 332 | 479 | 398 | 306 | 241 | 476 | 341 | 175 | 404 | 347 |
Labelled Cell Count | 223 | 490 | 241 | 261 | 384 | 307 | 387 | 412 | 500 | 602 | 524 | 462 | 345 | 312 | 368 | 560 | 458 | 349 | 241 | 476 | 341 | 175 | 404 | 347 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 5.4 | 5.5 | 5.5 | 6.1 | 5.0 | 5.1 | 5.3 | 4.5 | 4.5 | 4.2 | 5.0 | 5.7 | 5.9 | 5.7 | 7.4 | 7.6 | 7.5 | 5.0 | 5.0 | 5.6 |
Std Deviation (1e-4) | 0.8 | 0.6 | 1.1 | 1.2 | 1.1 | 1.3 | 1.3 | 0.8 | 1.1 | 1.1 | 1.0 | 1.4 | 1.5 | 1.5 | 1.4 | 1.5 | 1.7 | 1.7 | 1.3 | 1.7 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.14 | -0.13 | -0.1 | -0.07 | -0.29 | -0.28 | -0.4 | -0.15 | 0.05 | 0.1 | 0.06 | 0.43 | 0.47 | 0.44 | -0.15 | -0.12 | 0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -4.4 | -4.7 | -2.1 | -3.5 | -5.1 | -5.4 | -5.5 | -5.3 | -4.8 | -5.9 | -3.7 | -3.7 | -3.4 | -3.2 | -2.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 2.4 | 1.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 2.0 | 2.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.8 | 0.5 | 4.5 | 5.5 | 4.3 | 0 | 0 | 0 | 8.5 | 1.7 | 0 | 0 | 0 | 0 | 0.2 |
Nucleus | 3.0 | 1.4 | -0.3 | 0.8 | 1.4 | -0.6 | -2.2 | -1.7 | -2.6 | 1.0 | 0.3 | -0.8 | -2.7 | -0.9 | -1.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.4 | 4.2 | 2.8 | 1.6 | 3.4 | 1.3 | 2.9 | 1.4 | 3.7 | 5.1 | 4.6 | 6.1 | 7.0 | 5.1 | 4.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8786 | 3.7778 | 3.2323 | 2.5679 | 2.77 | 2.973 | 2.2818 | 2.337 | 1.9427 | 2.0103 | 1.8765 | 1.8414 | 1.5355 | 1.9365 | 1.7369 | 1.4674 | 1.9115 | 1.7668 |
Actin | 0.036 | 0.0113 | 0.0076 | 0.0245 | 0.0072 | 0.0047 | 0.0193 | 0.0002 | 0.0023 | 0.0185 | 0.0107 | 0.0033 | 0.003 | 0.0001 | 0.0023 | 0 | 0.0002 | 0 |
Bud | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0047 | 0.0029 | 0.0042 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0.0068 | 0.0004 | 0.0072 | 0.0003 | 0.001 | 0.0054 | 0.0027 | 0.0008 | 0.0009 | 0.0029 | 0.001 | 0.0041 | 0.0009 | 0.0003 | 0.0004 | 0.0003 | 0.0009 | 0.0039 |
Bud Periphery | 0.0009 | 0.0002 | 0.0004 | 0.0001 | 0.0026 | 0.0002 | 0.0009 | 0.0004 | 0.0011 | 0.0048 | 0.0042 | 0.0016 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.0034 | 0.0043 | 0.0036 | 0.0001 | 0.0005 | 0.0009 | 0.0067 | 0.0034 | 0.0013 | 0.0102 | 0.0101 | 0.0005 | 0.0003 | 0.0003 | 0.0004 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0007 | 0 | 0.0004 | 0 | 0.0003 | 0 | 0.0004 | 0.0004 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0148 | 0.0093 | 0.0214 | 0.0399 | 0.0052 | 0.0054 | 0.0063 | 0.0066 | 0.0037 | 0.0049 | 0.014 | 0.0037 | 0.0063 | 0.0111 | 0.0033 | 0.0007 | 0.0014 | 0.001 |
Cytoplasmic Foci | 0.0198 | 0.0098 | 0.0048 | 0.0076 | 0.0029 | 0.0054 | 0.0461 | 0.0003 | 0.005 | 0.007 | 0.0126 | 0.0012 | 0.0074 | 0.0002 | 0.0048 | 0 | 0 | 0.0001 |
Eisosomes | 0.0008 | 0.0001 | 0.0004 | 0.0002 | 0.0013 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.009 | 0.0003 | 0.0014 | 0.002 | 0.0005 | 0.0005 | 0.0055 | 0.0005 | 0.0007 | 0.0029 | 0.0009 | 0.001 | 0.0005 | 0.0003 | 0.0011 | 0 | 0.0002 | 0 |
Endosome | 0.0409 | 0.0019 | 0.0026 | 0.0064 | 0.0011 | 0.0027 | 0.0506 | 0.0018 | 0.0069 | 0.022 | 0.0107 | 0.0042 | 0.003 | 0.0002 | 0.0055 | 0 | 0.0003 | 0.0001 |
Golgi | 0.012 | 0.0025 | 0.0015 | 0.0166 | 0.0007 | 0.0037 | 0.0195 | 0.0001 | 0.0022 | 0.019 | 0.0067 | 0.0025 | 0.0008 | 0 | 0.0011 | 0 | 0 | 0 |
Lipid Particles | 0.0438 | 0.003 | 0.0033 | 0.0018 | 0.0011 | 0.0043 | 0.0296 | 0.0008 | 0.0058 | 0.0139 | 0.0016 | 0.0036 | 0.0022 | 0 | 0.0021 | 0 | 0 | 0 |
Mitochondria | 0.0128 | 0.002 | 0.0169 | 0.0006 | 0.0504 | 0.0041 | 0.0047 | 0.0014 | 0.0077 | 0.0264 | 0.0053 | 0.0103 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 |
None | 0.0237 | 0.0284 | 0.0309 | 0.1049 | 0.1932 | 0.0278 | 0.0025 | 0.0012 | 0.001 | 0.0117 | 0.0008 | 0.0009 | 0.0116 | 0.0068 | 0.0017 | 0.0002 | 0.0005 | 0.0001 |
Nuclear Periphery | 0.0175 | 0.0091 | 0.011 | 0.0082 | 0.0079 | 0.0054 | 0.0447 | 0.0268 | 0.0192 | 0.0164 | 0.0155 | 0.0128 | 0.0111 | 0.0143 | 0.0144 | 0.0021 | 0.0323 | 0.002 |
Nucleolus | 0.0099 | 0.0082 | 0.006 | 0.0051 | 0.007 | 0.0164 | 0.0068 | 0.0063 | 0.0033 | 0.0029 | 0.0168 | 0.0125 | 0.0069 | 0.0065 | 0.006 | 0.0047 | 0.0109 | 0.0118 |
Nucleus | 0.6882 | 0.8861 | 0.8542 | 0.7756 | 0.708 | 0.8963 | 0.6966 | 0.9425 | 0.904 | 0.7741 | 0.8556 | 0.9139 | 0.7783 | 0.8885 | 0.8135 | 0.9214 | 0.8435 | 0.876 |
Peroxisomes | 0.0098 | 0.0082 | 0.0116 | 0.0009 | 0.0007 | 0.0074 | 0.0239 | 0.0001 | 0.0158 | 0.0154 | 0.0157 | 0.0006 | 0.0036 | 0 | 0.0011 | 0 | 0 | 0 |
Punctate Nuclear | 0.0408 | 0.0143 | 0.0134 | 0.0046 | 0.0059 | 0.0085 | 0.0241 | 0.0048 | 0.0106 | 0.0357 | 0.0063 | 0.0208 | 0.1631 | 0.0696 | 0.1412 | 0.0703 | 0.1089 | 0.1046 |
Vacuole | 0.0058 | 0.0002 | 0.0011 | 0.0002 | 0.0009 | 0.0006 | 0.0063 | 0.0008 | 0.0029 | 0.0036 | 0.0056 | 0.0014 | 0.0003 | 0.0012 | 0.0003 | 0 | 0.0003 | 0.0001 |
Vacuole Periphery | 0.0022 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0.0002 | 0.0021 | 0.0005 | 0.0008 | 0.0041 | 0.0017 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0002 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.6709 | 7.5792 | 10.1769 | 15.4881 | 10.2074 | 11.7018 | 14.5968 | 18.2259 | 17.1633 | 13.3509 |
Translational Efficiency | 0.9807 | 0.904 | 0.9191 | 0.7684 | 0.6946 | 0.8118 | 0.6399 | 0.5639 | 0.6557 | 0.6536 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2330 | 1567 | 1089 | 1553 | 1620 | 1157 | 592 | 983 | 3950 | 2724 | 1681 | 2536 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 666.44 | 729.56 | 850.37 | 809.39 | 705.74 | 741.31 | 797.77 | 771.95 | 682.56 | 734.55 | 831.85 | 794.88 |
Standard Deviation | 74.60 | 118.06 | 201.52 | 116.21 | 92.62 | 101.14 | 130.34 | 101.73 | 84.70 | 111.34 | 181.45 | 112.31 |
Intensity Change Log 2 | — | 0.130552 | 0.351616 | 0.280360 | — | 0.070940 | 0.176836 | 0.129371 | — | 0.100200 | 0.264369 | 0.204678 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000047 | 0.000230 | 0.000751 | 0.000627 | 0.000085 | 0.000166 | 0.000187 | 0.000878 | 0.000063 | 0.000203 | 0.000552 | 0.000724 |
Bud Neck | 0.003099 | 0.004319 | 0.006622 | 0.014856 | 0.001685 | 0.004828 | 0.001346 | 0.005153 | 0.002519 | 0.004535 | 0.004764 | 0.011095 |
Bud Site | 0.001212 | 0.001134 | 0.005087 | 0.019511 | 0.001780 | 0.002308 | 0.000413 | 0.014713 | 0.001445 | 0.001633 | 0.003441 | 0.017651 |
Cell Periphery | 0.000102 | 0.000093 | 0.000211 | 0.000202 | 0.000206 | 0.000116 | 0.000820 | 0.000227 | 0.000145 | 0.000103 | 0.000426 | 0.000212 |
Cytoplasm | 0.272396* | 0.158642* | 0.192215* | 0.176244* | 0.141599* | 0.163368* | 0.104323* | 0.196531* | 0.218753* | 0.160649* | 0.161262* | 0.184108* |
Cytoplasmic Foci | 0.022950 | 0.013116 | 0.003597 | 0.005319 | 0.014245 | 0.019850 | 0.009330 | 0.004181 | 0.019380 | 0.015976 | 0.005616 | 0.004878 |
Eisosomes | 0.000024 | 0.000026 | 0.000034 | 0.000031 | 0.000024 | 0.000023 | 0.000200 | 0.000036 | 0.000024 | 0.000025 | 0.000092 | 0.000033 |
Endoplasmic Reticulum | 0.000732 | 0.004094 | 0.006639 | 0.005282 | 0.002344 | 0.002073 | 0.003057 | 0.004530 | 0.001393 | 0.003235 | 0.005378 | 0.004990 |
Endosome | 0.001026 | 0.001451 | 0.001430 | 0.006131 | 0.000687 | 0.001411 | 0.000500 | 0.001577 | 0.000887 | 0.001434 | 0.001103 | 0.004366 |
Golgi | 0.000347 | 0.000618 | 0.000126 | 0.007120 | 0.000103 | 0.000394 | 0.000037 | 0.000305 | 0.000247 | 0.000523 | 0.000095 | 0.004478 |
Lipid Particles | 0.002115 | 0.002546 | 0.001011 | 0.001007 | 0.002653 | 0.002180 | 0.001171 | 0.001152 | 0.002336 | 0.002391 | 0.001068 | 0.001064 |
Mitochondria | 0.003292 | 0.004128 | 0.001060 | 0.004533 | 0.002944 | 0.002698 | 0.000352 | 0.000962 | 0.003149 | 0.003520 | 0.000810 | 0.003149 |
Mitotic Spindle | 0.002090 | 0.002960 | 0.009563 | 0.043651 | 0.001327 | 0.006117 | 0.000255 | 0.006128 | 0.001777 | 0.004301 | 0.006285 | 0.029106 |
None | 0.008631 | 0.014699 | 0.036369 | 0.006446 | 0.010629 | 0.008605 | 0.031072 | 0.009358 | 0.009450 | 0.012110 | 0.034504 | 0.007575 |
Nuclear Periphery | 0.001857 | 0.004256 | 0.002535 | 0.003650 | 0.002868 | 0.003523 | 0.001043 | 0.002288 | 0.002271 | 0.003945 | 0.002009 | 0.003122 |
Nuclear Periphery Foci | 0.000369 | 0.001619 | 0.002798 | 0.001205 | 0.000905 | 0.001130 | 0.000315 | 0.001121 | 0.000589 | 0.001411 | 0.001924 | 0.001172 |
Nucleolus | 0.002199 | 0.005703 | 0.003092 | 0.001713 | 0.002814 | 0.004010 | 0.001758 | 0.001197 | 0.002451 | 0.004984 | 0.002622 | 0.001513 |
Nucleus | 0.671829* | 0.772312* | 0.705020* | 0.660753* | 0.808811* | 0.768958* | 0.830903* | 0.720802* | 0.728009* | 0.770888* | 0.749352* | 0.684029* |
Peroxisomes | 0.001604 | 0.001236 | 0.000340 | 0.000471 | 0.000649 | 0.002116 | 0.000213 | 0.000442 | 0.001212 | 0.001610 | 0.000296 | 0.000459 |
Vacuole | 0.003776 | 0.006091 | 0.021281 | 0.037538 | 0.003418 | 0.005764 | 0.012641 | 0.027977 | 0.003629 | 0.005952 | 0.018238 | 0.033832 |
Vacuole Periphery | 0.000303 | 0.000724 | 0.000219 | 0.003709 | 0.000224 | 0.000364 | 0.000064 | 0.000443 | 0.000271 | 0.000571 | 0.000164 | 0.002443 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -7.10 | -7.73 | -10.48 | -6.19 | 2.39 | -2.04 | -5.54 | -2.55 | -2.17 | -1.61 | -6.16 | -8.68 | -5.59 | -4.05 | 0.21 |
Bud Neck | -1.73 | -6.82 | -8.09 | -6.61 | -2.25 | -3.46 | -1.30 | -5.03 | -0.76 | -4.59 | -3.49 | -7.08 | -9.38 | -6.17 | -3.17 |
Bud Site | -0.06 | -5.81 | -7.45 | -7.50 | -4.02 | -0.68 | 1.78 | -4.08 | -3.86 | -4.68 | -0.63 | -4.76 | -8.33 | -8.20 | -5.81 |
Cell Periphery | 0.75 | -6.08 | -5.40 | -6.10 | 1.55 | 2.24 | -3.55 | 0.26 | -2.72 | 3.77 | 2.33 | -4.52 | -1.49 | -5.28 | 3.96 |
Cytoplasm | 15.76 | 14.92 | 17.90 | 1.00 | -0.38 | -2.60 | 7.75 | -3.28 | -0.70 | -9.66 | 10.93 | 15.40 | 11.62 | 0.37 | -5.24 |
Cytoplasmic Foci | 5.59 | 16.19 | 15.49 | 7.52 | -1.18 | -2.61 | 4.84 | 8.24 | 8.04 | 2.88 | 2.20 | 15.95 | 17.39 | 11.01 | 1.27 |
Eisosomes | -2.38 | -8.27 | -10.47 | -6.98 | 0.55 | 0.23 | -7.84 | -9.31 | -9.19 | 6.25 | -1.76 | -9.60 | -13.99 | -11.37 | 5.97 |
Endoplasmic Reticulum | -13.94 | -16.18 | -23.99 | -8.17 | 4.86 | 0.75 | -6.66 | -11.15 | -11.90 | -1.74 | -10.84 | -17.05 | -24.89 | -13.83 | 3.64 |
Endosome | -2.06 | -4.79 | -4.74 | -3.71 | -3.04 | -3.32 | -0.91 | -6.93 | -0.81 | -5.99 | -3.70 | -5.14 | -6.06 | -3.74 | -4.10 |
Golgi | -1.04 | 2.06 | -2.66 | -2.36 | -2.91 | -2.10 | 5.00 | -0.94 | 1.14 | -1.49 | -1.79 | 2.35 | -2.73 | -2.22 | -3.00 |
Lipid Particles | -1.67 | 4.02 | 4.90 | 5.89 | 0.29 | 1.19 | 4.32 | 4.43 | 3.66 | -0.18 | -0.40 | 5.89 | 6.58 | 6.93 | 0.10 |
Mitochondria | -0.76 | 2.67 | -0.20 | 0.72 | -5.03 | 0.18 | 3.15 | 2.45 | 3.59 | -5.76 | -0.53 | 3.93 | 1.10 | 1.87 | -5.63 |
Mitotic Spindle | -0.63 | -4.70 | -7.77 | -7.41 | -3.35 | -2.45 | 1.20 | -2.79 | -0.26 | -3.37 | -2.32 | -4.49 | -8.23 | -6.51 | -3.93 |
None | -3.78 | -4.77 | 4.23 | 6.47 | 6.10 | 1.15 | -3.03 | 1.57 | 0.46 | 3.76 | -2.27 | -5.57 | 3.84 | 5.60 | 7.08 |
Nuclear Periphery | -5.85 | -7.83 | -13.96 | -1.77 | -2.71 | -1.48 | 4.47 | -1.80 | 0.43 | -8.14 | -5.58 | -5.02 | -12.12 | -1.40 | -4.78 |
Nuclear Periphery Foci | -5.05 | -6.02 | -4.80 | 2.21 | 4.19 | -0.22 | 2.43 | -1.62 | -0.85 | -6.14 | -3.57 | -4.91 | -4.42 | 0.91 | 2.79 |
Nucleolus | -5.36 | -1.40 | 2.36 | 7.05 | 4.15 | -2.09 | 3.38 | 4.85 | 4.94 | 1.86 | -5.45 | 0.51 | 4.69 | 8.54 | 4.63 |
Nucleus | -11.47 | -5.74 | -1.91 | 8.37 | 3.70 | 4.06 | -3.31 | 8.81 | 4.44 | 10.42 | -6.38 | -5.51 | 3.98 | 9.33 | 8.27 |
Peroxisomes | 0.77 | 3.27 | 3.35 | 4.55 | 0.43 | -2.76 | 0.77 | 1.51 | 3.19 | 0.09 | -1.28 | 3.36 | 3.65 | 4.95 | 0.24 |
Vacuole | -4.86 | -12.88 | -19.03 | -18.03 | -7.01 | -2.69 | -6.34 | -14.00 | -13.28 | -6.44 | -5.29 | -14.12 | -23.62 | -22.38 | -9.37 |
Vacuole Periphery | -1.92 | 0.96 | -3.10 | -2.00 | -3.27 | -2.30 | 4.43 | -0.99 | 0.52 | -2.70 | -2.35 | 2.13 | -3.21 | -1.97 | -3.55 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|