Standard name | |
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Human Ortholog | |
Description | Component of the RNA polymerase II mediator complex; mediator is required for transcriptional activation and also has a role in basal transcription |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 0 | 0 | 1 | 4 | 8 | 4 | – | 6 | 4 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 2 | 2 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | – | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 5 | 2 | 4 | 18 | 23 | 22 | 17 | – | 9 | 17 | 0 | 0 | 4 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 8 | 12 | 7 | 14 | 8 | 19 | 24 | 8 | – | 11 | 10 | 20 | 40 | 49 | 12 | 16 | 12 | 0 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 3 | 6 | 6 | 0 | 0 | 3 | 0 | 2 | 1 |
Endosome | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 2 | 5 | 0 | 0 | 2 | 3 | 5 | 1 | 2 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 0 | 0 | 0 | 3 | 0 | 2 | 1 |
Mitochondria | 0 | 7 | 65 | 74 | 61 | 147 | 189 | 334 | 204 | – | 163 | 187 | 2 | 0 | 4 | 6 | 2 | 20 | 0 | 2 | 4 | 0 | 3 | 4 |
Nucleus | 117 | 318 | 389 | 312 | 423 | 366 | 468 | 639 | 279 | – | 259 | 260 | 185 | 244 | 281 | 227 | 181 | 175 | 109 | 327 | 386 | 151 | 162 | 167 |
Nuclear Periphery | 0 | 3 | 4 | 3 | 4 | 4 | 15 | 10 | 7 | – | 10 | 8 | 1 | 1 | 4 | 7 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 27 | 46 | 19 | 30 | 41 | 55 | 88 | 157 | 88 | – | 87 | 115 | 7 | 21 | 20 | 9 | 7 | 10 | 28 | 33 | 27 | 11 | 31 | 49 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2 | 3 | 0 | 2 | 3 | 3 | 2 | 4 | – | 0 | 0 | 4 | 5 | 4 | 1 | 5 | 2 | 0 | 2 | 3 | 2 | 2 | 4 |
Vac/Vac Membrane | 2 | 5 | 3 | 1 | 4 | 1 | 2 | 7 | 3 | – | 2 | 3 | 1 | 6 | 3 | 9 | 10 | 5 | 5 | 9 | 19 | 5 | 16 | 23 |
Unique Cell Count | 128 | 355 | 434 | 342 | 473 | 436 | 591 | 858 | 406 | 349 | 374 | 211 | 302 | 345 | 269 | 214 | 196 | 150 | 382 | 461 | 177 | 229 | 268 | |
Labelled Cell Count | 150 | 391 | 503 | 430 | 553 | 604 | 812 | 1205 | 615 | 548 | 605 | 221 | 321 | 375 | 288 | 233 | 232 | 150 | 382 | 461 | 177 | 229 | 268 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.0 | 8.2 | 5.4 | 5.0 | 5.3 | 4.6 | 4.6 | 4.3 | 4.2 | – | 4.2 | 4.1 | 7.7 | 7.9 | 7.4 | 9.3 | 9.7 | 8.4 | 8.6 | 8.8 | 9.2 |
Std Deviation (1e-4) | 2.5 | 1.6 | 1.1 | 1.1 | 1.4 | 1.1 | 1.3 | 1.1 | 1.1 | – | 1.3 | 1.1 | 2.3 | 2.5 | 3.0 | 2.1 | 2.6 | 2.2 | 1.7 | 1.7 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.02 | -0.24 | -0.23 | -0.31 | -0.36 | – | -0.37 | -0.41 | 0.51 | 0.54 | 0.45 | 0.79 | 0.84 | 0.64 | 0.67 | 0.71 | 0.77 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | 0.2 | -0.9 | 0.4 | 0 | -0.8 | – | 0.3 | -0.1 | 3.7 | 5.5 | 5.9 | 1.2 | 2.8 | 2.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.4 | -0.9 | 0 | 0 | 0 | 0 | – | 0 | 0 | -5.5 | -7.0 | -6.7 | -5.5 | -5.5 | -1.6 |
Nucleus | 0.7 | -0.1 | -2.5 | -4.5 | -6.4 | -7.5 | – | -5.7 | -7.2 | -0.7 | -3.4 | -3.3 | -2.1 | -1.9 | -0.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.5 | 2.6 | 0 | 0 | 0 | 0 | – | 0 | 0 | -0.6 | 1.5 | 0.9 | -0.7 | -0.7 | 0.4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.1476 | 5.4193 | 5.0534 | 4.719 | 4.2923 | 5.1943 | 3.0219 | 2.8014 | 2.7976 | 1.9459 | 2.5038 | 2.512 | 8.4558 | 8.2296 | 8.0985 | 7.1354 | 7.6065 | 8.1994 |
Actin | 0.0241 | 0 | 0.0278 | 0.0324 | 0 | 0.0013 | 0.0272 | 0 | 0.0007 | 0.0001 | 0.0054 | 0.0001 | 0.0079 | 0 | 0.0005 | 0 | 0.0002 | 0 |
Bud | 0.0005 | 0 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0.002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Bud Neck | 0.0029 | 0.0001 | 0.0009 | 0.002 | 0.0003 | 0.001 | 0.0015 | 0 | 0.0008 | 0.0001 | 0.0014 | 0.0006 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0073 | 0.0009 |
Bud Periphery | 0.0007 | 0 | 0.0003 | 0.0006 | 0 | 0.0001 | 0.0015 | 0 | 0.0003 | 0 | 0.0017 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0 |
Bud Site | 0.0063 | 0 | 0.0009 | 0.0045 | 0 | 0.0001 | 0.001 | 0 | 0.0004 | 0 | 0.0035 | 0.0002 | 0.0003 | 0 | 0.0003 | 0 | 0.0011 | 0 |
Cell Periphery | 0.0003 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 |
Cytoplasm | 0.0077 | 0 | 0.0005 | 0.0009 | 0.0001 | 0.0001 | 0.0018 | 0 | 0.0002 | 0.0001 | 0.004 | 0.0005 | 0.0003 | 0 | 0.0013 | 0 | 0.0005 | 0 |
Cytoplasmic Foci | 0.0054 | 0 | 0.0008 | 0.006 | 0 | 0.0005 | 0.0045 | 0 | 0.0001 | 0 | 0.0005 | 0.0005 | 0.0011 | 0 | 0.0012 | 0 | 0.0005 | 0 |
Eisosomes | 0.0002 | 0 | 0.0006 | 0.0014 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0075 | 0 | 0.0007 | 0.0014 | 0 | 0.0001 | 0.0012 | 0 | 0.0001 | 0 | 0.0008 | 0.0004 | 0.0004 | 0 | 0.0005 | 0 | 0.0005 | 0 |
Endosome | 0.0273 | 0 | 0.0018 | 0.0072 | 0 | 0.0013 | 0.0063 | 0 | 0.0009 | 0 | 0.0017 | 0.0013 | 0.0023 | 0 | 0.0023 | 0 | 0.0047 | 0 |
Golgi | 0.0041 | 0 | 0.0015 | 0.0059 | 0 | 0.0006 | 0.0032 | 0 | 0.0006 | 0 | 0.0003 | 0.0001 | 0.0006 | 0 | 0.0002 | 0 | 0.0005 | 0 |
Lipid Particles | 0.0056 | 0 | 0.0017 | 0.0087 | 0 | 0.002 | 0.0031 | 0 | 0.0002 | 0 | 0.0001 | 0.0013 | 0.0036 | 0 | 0.0006 | 0 | 0.0018 | 0 |
Mitochondria | 0.0034 | 0.0001 | 0.0017 | 0.0039 | 0.0001 | 0.0002 | 0.0031 | 0.0001 | 0.0062 | 0.0001 | 0.003 | 0.0135 | 0.0008 | 0 | 0.0005 | 0 | 0.0007 | 0 |
None | 0.0061 | 0 | 0.0013 | 0.0044 | 0 | 0.0001 | 0.0008 | 0 | 0.0001 | 0 | 0.0003 | 0.0006 | 0.0005 | 0 | 0.0018 | 0 | 0.0003 | 0 |
Nuclear Periphery | 0.0381 | 0.0004 | 0.0007 | 0.009 | 0.0015 | 0.0027 | 0.0095 | 0.0003 | 0.0003 | 0.0072 | 0.0086 | 0.0046 | 0.0014 | 0.0002 | 0.004 | 0.0001 | 0.0059 | 0.0002 |
Nucleolus | 0.034 | 0.0316 | 0.0162 | 0.0102 | 0.0835 | 0.0849 | 0.0298 | 0.0236 | 0.025 | 0.0104 | 0.0742 | 0.0684 | 0.0303 | 0.0315 | 0.0314 | 0.0239 | 0.1747 | 0.0799 |
Nucleus | 0.7963 | 0.9673 | 0.94 | 0.8902 | 0.9105 | 0.9013 | 0.888 | 0.9751 | 0.961 | 0.9813 | 0.888 | 0.9029 | 0.9449 | 0.9677 | 0.946 | 0.9755 | 0.7916 | 0.9173 |
Peroxisomes | 0.0041 | 0 | 0.0015 | 0.0071 | 0 | 0.0004 | 0.0089 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0006 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Punctate Nuclear | 0.0177 | 0.0005 | 0.0008 | 0.0023 | 0.0039 | 0.003 | 0.0044 | 0.0008 | 0.0013 | 0.0006 | 0.0022 | 0.0033 | 0.0028 | 0.0002 | 0.0081 | 0.0002 | 0.0049 | 0.0014 |
Vacuole | 0.0048 | 0 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0024 | 0 | 0.0008 | 0 | 0.0014 | 0.0006 | 0.0011 | 0 | 0.0004 | 0 | 0.0033 | 0 |
Vacuole Periphery | 0.0028 | 0 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0009 | 0 | 0.0007 | 0.0001 | 0.0006 | 0.0005 | 0.0004 | 0 | 0.0002 | 0 | 0.0007 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.1212 | 15.3804 | 15.0737 | 22.6173 | 15.1521 | 13.3023 | 20.3925 | 24.2595 | 17.987 | 23.7135 |
Translational Efficiency | 0.8147 | 0.7979 | 0.7354 | 0.4596 | 0.6741 | 0.7849 | 0.6015 | 0.418 | 0.6373 | 0.5041 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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