Standard name
Human Ortholog
Description Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; required for regulation of copper genes in response to DNA-damaging reagents; CUP2 has a paralog, HAA1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0.15 0.16 0.14 0.18 – 0.12 0.08 0.11 0.09 0.35 0.31 0.25 0.12 0.23 0.19 0.06 0.06 0.1 0.18 0.17 0.23
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 0 0.06 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.39 – 0.14 0 0.5 0.18 – 0.54 0.42 0.57 0.42 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.45 0.33 – 0.69 0.74 0.61 0.62 – 0.61 0.67 0.6 0.66 0.4 0.26 0.41 0.6 0.56 0.54 0.57 0.65 0.57 0.48 0.4 0.31
Nuclear Periphery 0.08 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.46 0.61 – 0.11 0.13 0.06 0.1 – 0.06 0.07 0.05 0.07 0.41 0.54 0.44 0.32 0.27 0.3 0.26 0.14 0.22 0.21 0.24 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3
Bud 0 0 – 0 0 1 2 – 5 0 4 6 0 0 0 0 0 0 1 1 0 6 10 14
Bud Neck 0 0 – 1 1 1 0 – 2 1 0 0 0 0 0 0 0 0 0 0 0 0 3 8
Bud Site 0 0 – 0 0 5 4 – 11 4 7 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 0 – 27 38 35 78 – 38 31 40 49 77 101 96 17 26 15 8 3 5 41 53 79
Endoplasmic Reticulum 0 0 – 0 3 1 0 – 0 0 0 0 1 0 0 4 5 4 0 0 0 1 1 3
Endosome 1 0 – 2 2 0 6 – 1 0 1 3 3 18 16 5 1 4 2 1 0 0 10 9
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 2 2 4
Mitochondria 12 7 – 25 3 128 78 – 175 170 206 221 4 0 0 3 3 0 0 0 0 5 8 7
Nucleus 38 6 – 120 180 157 275 – 197 271 219 341 89 84 159 84 63 43 78 40 29 108 128 104
Nuclear Periphery 7 0 – 0 0 0 1 – 3 4 0 3 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 2 0 – 0 0 0 2 – 0 1 0 4 1 0 0 0 1 0 1 0 0 1 3 6
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 3 1 2 10 – 1 7 2 6 0 0 0 1 0 0 3 0 1 3 11 14
Vac/Vac Membrane 39 11 – 20 31 16 44 – 18 28 18 38 92 176 169 45 30 24 35 8 11 47 77 73
Unique Cell Count 84 18 175 243 258 440 325 403 363 520 222 323 384 140 113 79 138 63 52 227 319 338
Labelled Cell Count 101 24 198 259 346 501 451 517 497 680 267 379 440 159 129 91 138 63 52 227 319 338


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.0 – 5.2 5.7 3.8 5.0 – 4.1 4.4 4.2 4.3 5.8 6.0 6.1 9.2 9.4 9.0 5.3 5.4 5.8
Std Deviation (1e-4) 1.0 1.2 – 1.2 1.5 1.0 1.5 – 1.3 1.1 1.7 1.1 0.9 1.0 1.3 1.7 1.9 1.9 1.5 1.4 1.5
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2653 -0.4907 -0.6811 -0.6276 -1.0416 -0.5264 1.3178 1.1073 0.9574 0.8659 0.0961 0.7912 3.6277 2.8606 2.8442 2.7521 2.511 2.8346
Actin 0.0702 0.0062 0.0053 0.0028 0.0013 0.0002 0.0426 0.0006 0.0041 0.0043 0.0003 0.0008 0.0578 0.0004 0.0002 0.0006 0.0599 0.0017
Bud 0.0008 0.0005 0.0005 0.0006 0.0006 0.0001 0.001 0.0041 0.0015 0.0185 0.0004 0.0004 0.0008 0.0006 0.0003 0.0002 0.0001 0.0003
Bud Neck 0.0021 0.0005 0.0009 0.0004 0.0044 0.0033 0.0011 0.0022 0.0026 0.0063 0.0213 0.0083 0.0404 0.0005 0.0003 0.0005 0.0007 0.0104
Bud Periphery 0.0008 0.0005 0.0005 0.0011 0.0006 0.0001 0.0015 0.0006 0.0006 0.005 0.0003 0.0006 0.0012 0.0003 0.0003 0.0001 0.0001 0.0005
Bud Site 0.0052 0.0023 0.0026 0.002 0.0133 0.0001 0.0055 0.0054 0.0023 0.0021 0.0208 0.0004 0.0038 0.0016 0.0005 0.0003 0.0005 0.0003
Cell Periphery 0.0003 0.0001 0.0002 0.0004 0.0003 0 0.0003 0.0001 0.0001 0.0003 0.0002 0.0001 0.0005 0.0001 0 0 0 0.0001
Cytoplasm 0.0769 0.2994 0.2048 0.108 0.1915 0.2115 0.0762 0.2729 0.1427 0.1204 0.2046 0.1412 0.1307 0.222 0.1928 0.1728 0.085 0.1378
Cytoplasmic Foci 0.0559 0.0046 0.0084 0.0065 0.0315 0.0067 0.0402 0.0068 0.0054 0.0223 0.0169 0.0073 0.011 0.0034 0.0029 0.0019 0.0046 0.0021
Eisosomes 0.0005 0 0.0001 0.0004 0.0001 0 0.0004 0 0 0.0001 0 0 0.0017 0 0 0.0001 0.0002 0
Endoplasmic Reticulum 0.0059 0.0145 0.0135 0.0042 0.0018 0.0036 0.0044 0.0076 0.0052 0.004 0.0027 0.0031 0.0128 0.0053 0.003 0.0032 0.001 0.0021
Endosome 0.0857 0.0109 0.0245 0.0279 0.0163 0.0184 0.0617 0.0117 0.0132 0.0247 0.0233 0.0136 0.027 0.0065 0.0022 0.0024 0.0012 0.0019
Golgi 0.0596 0.0002 0.0031 0.012 0.0097 0.0008 0.0137 0.0011 0.0056 0.0187 0.006 0.0003 0.005 0.0005 0.0001 0.0001 0.0012 0.0001
Lipid Particles 0.0287 0.0008 0.0018 0.0051 0.0147 0.0002 0.0235 0.0019 0.0022 0.0191 0.019 0.0003 0.0036 0.0003 0.0002 0.0003 0.0013 0.0001
Mitochondria 0.0336 0.0015 0.0034 0.1569 0.0508 0.0006 0.0386 0.0064 0.0084 0.0683 0.001 0.001 0.0036 0.0018 0.0007 0.0011 0.0004 0.0006
None 0.0473 0.1015 0.1041 0.0976 0.007 0.0752 0.0281 0.0932 0.0846 0.1044 0.0606 0.0521 0.2776 0.101 0.1607 0.1216 0.1409 0.0643
Nuclear Periphery 0.0236 0.0686 0.0725 0.0419 0.0115 0.0098 0.0167 0.0339 0.0385 0.0315 0.0137 0.0198 0.0254 0.0175 0.0135 0.0129 0.0055 0.0097
Nucleolus 0.0021 0.0021 0.0019 0.002 0.0116 0.0025 0.004 0.0027 0.0029 0.0078 0.0097 0.0042 0.0034 0.0028 0.0025 0.0049 0.0016 0.004
Nucleus 0.4552 0.4565 0.5151 0.5053 0.6117 0.6561 0.5819 0.5215 0.647 0.4735 0.5581 0.7179 0.3688 0.6154 0.5982 0.655 0.6854 0.7453
Peroxisomes 0.031 0.0003 0.0012 0.0057 0.0096 0.0001 0.0306 0.0041 0.0084 0.0115 0.0027 0.0004 0.0016 0.0002 0.0005 0.0001 0.0053 0.0001
Punctate Nuclear 0.0074 0.0183 0.0214 0.0137 0.0037 0.0067 0.021 0.0147 0.0178 0.0534 0.0336 0.0244 0.0185 0.0153 0.0179 0.0185 0.0047 0.0167
Vacuole 0.0046 0.0089 0.0119 0.0034 0.0072 0.0033 0.0055 0.0073 0.0056 0.0025 0.0045 0.0031 0.0034 0.0037 0.0026 0.0028 0.0004 0.0017
Vacuole Periphery 0.0028 0.0017 0.0026 0.0019 0.0009 0.0007 0.0017 0.001 0.0012 0.0013 0.0005 0.0006 0.0013 0.0007 0.0005 0.0005 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.2744 11.6387 12.9546 19.2268 13.7153 14.6389 13.9743 14.8922 25.6529 17.1315
Translational Efficiency 1.2996 1.8502 1.394 0.7197 1.7293 1.9929 1.2613 1.8594 1.0346 1.3785

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1807 263 587 1269 141 1602 1561 815 1948 1865 2148 2084

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 724.59 791.52 998.44 986.72 759.98 803.65 901.99 1032.30 727.15 801.94 928.35 1004.55
Standard Deviation 95.32 106.64 118.84 158.58 78.43 116.00 138.93 173.14 94.64 114.80 140.48 165.93
Intensity Change Log 2 — 0.127461 0.462511 0.445476 — 0.080606 0.247150 0.441829 — 0.103665 0.356281 0.443610

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000444 0.001506 0.000761 0.001034 0.000123 0.000276 0.000411 0.002239 0.000421 0.000449 0.000507 0.001505
Bud Neck 0.006329 0.013696 0.005576 0.022336 0.000585 0.009796 0.006117 0.026939 0.005913 0.010346 0.005970 0.024136
Bud Site 0.002392 0.011244 0.006716 0.023056 0.000388 0.009871 0.001724 0.033557 0.002247 0.010064 0.003088 0.027162
Cell Periphery 0.000156 0.000215 0.000138 0.000321 0.000132 0.000177 0.000109 0.000381 0.000155 0.000182 0.000117 0.000344
Cytoplasm 0.176578* 0.186616* 0.025698 0.052734 0.106731* 0.187392* 0.043706 0.049156 0.171522* 0.187282* 0.038785 0.051335
Cytoplasmic Foci 0.029300 0.039962 0.000682 0.005795 0.036406 0.022392 0.000466 0.007990 0.029814 0.024870 0.000525 0.006653
Eisosomes 0.000037 0.000079 0.000035 0.000029 0.000058 0.000028 0.000014 0.000034 0.000038 0.000035 0.000020 0.000031
Endoplasmic Reticulum 0.001542 0.006477 0.007559 0.006845 0.001952 0.001892 0.004163 0.007229 0.001572 0.002539 0.005091 0.006995
Endosome 0.001099 0.003858 0.000818 0.009798 0.002288 0.002428 0.000575 0.008876 0.001185 0.002630 0.000641 0.009437
Golgi 0.000330 0.002234 0.000080 0.008258 0.000136 0.001477 0.000251 0.008176 0.000316 0.001584 0.000204 0.008226
Lipid Particles 0.002708 0.003440 0.000507 0.000738 0.003064 0.002076 0.000182 0.000499 0.002734 0.002268 0.000271 0.000644
Mitochondria 0.002197 0.002203 0.000934 0.011946 0.000564 0.004514 0.001036 0.010769 0.002078 0.004188 0.001008 0.011486
Mitotic Spindle 0.001150 0.011947 0.001802 0.057725 0.003674 0.008431 0.005089 0.071123 0.001333 0.008927 0.004191 0.062965
None 0.008066 0.011183 0.005303 0.009430 0.004923 0.009673 0.007342 0.014499 0.007839 0.009886 0.006784 0.011413
Nuclear Periphery 0.001395 0.000867 0.001003 0.002238 0.000793 0.000996 0.000815 0.001826 0.001352 0.000978 0.000866 0.002076
Nuclear Periphery Foci 0.000477 0.001786 0.000377 0.002548 0.002890 0.000706 0.000719 0.001961 0.000651 0.000858 0.000626 0.002318
Nucleolus 0.002380 0.004716 0.001738 0.001911 0.002591 0.004441 0.000553 0.002426 0.002395 0.004479 0.000877 0.002113
Nucleus 0.755387* 0.666747* 0.930281* 0.752648* 0.822222* 0.719276* 0.922051* 0.729752* 0.760225* 0.711868* 0.924300* 0.743694*
Peroxisomes 0.001893 0.005973 0.000296 0.000968 0.001157 0.003499 0.000090 0.000896 0.001840 0.003848 0.000147 0.000940
Vacuole 0.005796 0.024646 0.009493 0.023146 0.009150 0.009490 0.004406 0.019212 0.006039 0.011627 0.005796 0.021608
Vacuole Periphery 0.000342 0.000604 0.000204 0.006496 0.000173 0.001170 0.000181 0.002461 0.000330 0.001090 0.000187 0.004918

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.60 -1.47 -1.85 0.69 -0.78 -2.96 -10.55 -8.01 -7.07 -6.17 -0.13 -0.67 -2.79 -6.99 -7.13
Bud Neck -2.31 -0.88 -8.33 -1.89 -8.48 -8.02 -10.90 -12.14 -7.15 -8.41 -3.30 -2.00 -11.67 -7.91 -11.45
Bud Site -3.02 -1.68 -5.13 0.00 -2.16 -6.42 -6.06 -6.61 -2.39 -5.84 -5.45 -1.19 -7.49 -1.80 -6.79
Cell Periphery -2.03 0.74 -1.61 0.76 -1.99 -1.71 1.31 -2.57 -1.58 -3.57 -1.33 2.14 -2.42 -1.32 -4.90
Cytoplasm -0.61 26.82 25.06 10.65 -3.77 -5.51 5.02 5.17 22.34 0.76 -2.25 26.62 26.11 26.33 -0.61
Cytoplasmic Foci -1.68 15.43 14.41 6.47 -2.44 1.87 4.96 4.61 9.87 -2.92 2.11 16.31 14.99 12.43 -3.97
Eisosomes -4.27 -1.01 -0.01 4.45 1.38 4.04 5.39 1.26 -7.35 -12.27 0.75 4.19 -0.96 -2.55 -10.58
Endoplasmic Reticulum -3.67 -14.33 -15.45 -1.00 2.17 0.23 -10.35 -13.04 -12.96 -7.22 -3.35 -18.51 -20.40 -14.11 -5.60
Endosome -2.82 1.53 -3.28 0.52 -3.85 -0.21 1.81 -1.02 -1.29 -4.29 -3.82 3.08 -4.29 -1.17 -5.80
Golgi -1.50 3.63 -2.97 -0.30 -3.39 -4.23 -0.40 -2.60 -1.07 -2.53 -3.77 1.90 -3.80 -1.44 -4.14
Lipid Particles -1.10 6.04 6.30 4.50 0.66 1.45 5.01 4.15 3.66 -5.68 1.00 7.52 6.65 5.02 -4.65
Mitochondria -0.02 2.35 -2.57 -2.56 -3.82 -5.11 -1.93 -4.16 -0.72 -3.74 -2.66 2.11 -3.52 -1.21 -5.18
Mitotic Spindle -1.91 -1.21 -7.71 -3.10 -7.10 -1.43 -1.12 -7.13 -7.04 -7.29 -4.48 -3.72 -11.34 -8.20 -9.46
None -1.36 2.89 0.40 1.65 -3.11 -2.72 -0.86 -2.30 -0.09 -2.11 -1.69 1.96 -0.56 1.32 -3.04
Nuclear Periphery 2.94 0.27 -7.57 -9.12 -8.16 -1.10 -1.96 -9.06 -10.85 -9.72 3.23 1.16 -10.17 -13.95 -12.32
Nuclear Periphery Foci -2.10 0.72 -1.74 1.31 -2.15 1.01 1.11 0.67 -1.43 -1.74 -0.96 0.57 -1.74 -0.76 -2.13
Nucleolus -2.24 2.43 1.62 3.06 -0.86 -2.19 3.25 -1.09 1.50 -6.24 -2.81 4.55 0.38 3.25 -6.11
Nucleus 4.51 -20.29 -7.87 -8.09 10.56 4.70 -4.71 1.69 -4.98 12.43 5.23 -23.50 -6.08 -10.82 16.23
Peroxisomes -3.13 5.40 4.96 4.57 -0.84 -3.29 2.71 1.75 5.05 -3.05 -3.10 6.10 5.10 6.34 -3.22
Vacuole -4.95 -5.15 -10.13 -0.55 -5.15 -0.16 1.08 -7.44 -8.00 -8.74 -5.62 -3.95 -13.44 -9.57 -11.35
Vacuole Periphery -1.84 2.15 -2.97 -2.50 -3.28 -2.38 0.50 -3.46 0.40 -3.57 -2.07 2.43 -3.55 -1.10 -4.09
Standard name
Human Ortholog
Description Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; required for regulation of copper genes in response to DNA-damaging reagents; CUP2 has a paralog, HAA1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (47%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Cup2

Cup2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cup2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available