Standard name
Human Ortholog
Description Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0 0.05 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.05 0.07 0.06 0 0.08 0.07 0.11 0.12 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.9 0.96 0.93 0.97 0.87 0.83 0.76 0.73 0.67 0.63 0.57 0.57 0.97 0.97 0.94 0.97 0.95 0.97 0.96 0.9 0.9 0.92 0.89 0.88
Endoplasmic Reticulum 0 0 0.05 0 0.08 0.1 0.11 0.14 0.09 0.14 0.09 0.11 0 0 0.05 0 0 0 0 0 0 0.05 0.08 0.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.13 0.1 0.26 0.25 0.37 0.34 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 1 8 20 20 18 21 20 25 50 0 0 2 0 0 0 0 1 1 0 1 0
Bud 0 1 1 1 1 5 5 3 5 2 15 3 0 0 1 0 0 0 4 2 2 1 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 11 5 22 9 33 38 40 30 33 35 49 68 3 4 14 6 17 11 0 0 0 0 0 0
Cytoplasm 299 137 573 398 588 473 521 541 280 310 261 320 292 300 299 284 296 369 358 144 594 229 308 358
Endoplasmic Reticulum 9 5 30 11 55 57 77 103 37 68 42 61 10 8 15 12 11 10 1 2 29 13 28 40
Endosome 0 0 0 0 0 0 1 1 2 0 0 0 0 0 0 0 2 0 0 0 2 0 0 0
Golgi 2 0 0 0 15 1 6 3 2 1 4 0 0 1 0 0 3 1 0 1 3 0 0 0
Mitochondria 1 0 0 4 7 41 90 75 111 125 168 193 3 0 2 1 3 0 1 0 3 0 0 0
Nucleus 1 0 0 0 0 1 1 3 0 2 0 1 0 1 0 2 3 3 0 1 1 0 0 0
Nuclear Periphery 0 0 0 1 0 0 0 1 1 1 2 2 0 0 0 0 0 0 1 0 2 0 0 0
Nucleolus 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 23 1 14 1 18 4 15 25 12 4 16 8 0 1 6 8 12 5 2 5 13 1 1 2
Unique Cell Count 331 142 613 411 676 568 688 740 421 492 457 565 300 310 317 294 313 381 375 161 662 250 348 406
Labelled Cell Count 346 149 643 426 725 640 776 803 505 568 582 706 308 315 339 313 347 399 375 161 662 250 348 406


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 34.2 30.3 21.8 19.5 19.2 15.7 14.3 13.1 11.9 11.3 10.6 10.3 29.1 27.5 23.9 29.2 31.4 31.5 31.4 34.4 36.4
Std Deviation (1e-4) 9.1 7.3 4.5 3.8 3.3 2.9 2.9 2.6 2.8 2.5 2.3 2.2 5.7 5.3 5.0 7.9 8.5 7.3 6.8 8.4 8.4
Intensity Change (Log2) – – – -0.16 -0.18 -0.48 -0.61 -0.73 -0.88 -0.94 -1.04 -1.08 0.42 0.33 0.13 0.42 0.53 0.53 0.52 0.66 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 4.1 3.6 3.2 5.0 4.4 5.3 7.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 4.2 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.3 1.1 2.4 1.9 0.4 3.0 2.6 4.6 5.5 -2.3 -2.0 0.6 -1.3 1.3 -0.6
Cytoplasm 2.4 -3.9 -5.5 -8.7 -9.8 -11.2 -12.6 -14.2 -14.7 2.5 2.1 0.5 1.9 0.7 2.3
Endoplasmic Reticulum -1.8 2.3 3.4 4.1 5.6 2.5 5.2 2.8 3.8 -1.1 -1.7 -0.1 -0.5 -1.0 -1.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 3.7 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.8 9.3 8.1 13.5 13.3 16.3 15.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.7 0.4 -2.2 -0.1 1.2 0.6 -1.9 1.2 -1.1 0 -2.2 -0.4 0.4 1.4 -1.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0443 0.001 0.0233 0.0133 0.0022 0.002 – – – – – – 0.0047 0.0002 0.0104 0.0004 0.0151 0.001
Bud 0.0015 0.0008 0.0029 0.0039 0.0012 0.0016 – – – – – – 0.0012 0.0003 0.0021 0.001 0.0005 0.0007
Bud Neck 0.0041 0.0011 0.005 0.0019 0.0013 0.0021 – – – – – – 0.0006 0.0003 0.0006 0.0008 0.0017 0.001
Bud Periphery 0.0023 0.0006 0.0022 0.0024 0.0014 0.0014 – – – – – – 0.0019 0.0001 0.0031 0.0003 0.0003 0.0003
Bud Site 0.0081 0.0012 0.0085 0.0029 0.0003 0.0003 – – – – – – 0.0007 0.0002 0.0021 0.0002 0.0011 0.0001
Cell Periphery 0.0045 0.0048 0.0072 0.0014 0.0004 0.0015 – – – – – – 0.0012 0.0002 0.0005 0.0003 0.0003 0.0002
Cytoplasm 0.582 0.9088 0.7701 0.7249 0.6762 0.7443 – – – – – – 0.8658 0.976 0.9097 0.8784 0.8539 0.8969
Cytoplasmic Foci 0.0096 0.0023 0.0095 0.0066 0.007 0.0092 – – – – – – 0.0112 0.0026 0.0065 0.0076 0.0326 0.0107
Eisosomes 0.0006 0 0.0005 0.0001 0.0001 0 – – – – – – 0.0001 0 0 0 0.0007 0
Endoplasmic Reticulum 0.1051 0.0286 0.036 0.0144 0.0204 0.11 – – – – – – 0.0312 0.0084 0.012 0.0258 0.0114 0.026
Endosome 0.0338 0.0076 0.0206 0.0204 0.0441 0.0461 – – – – – – 0.022 0.0043 0.0164 0.0294 0.0241 0.0341
Golgi 0.0106 0.0013 0.0031 0.0036 0.0058 0.0058 – – – – – – 0.0052 0.0007 0.0034 0.0012 0.0037 0.0099
Lipid Particles 0.0063 0.0003 0.0045 0.0099 0.0009 0.0004 – – – – – – 0.0017 0.0001 0.0004 0.0001 0.008 0.0001
Mitochondria 0.0382 0.0013 0.005 0.0471 0.0125 0.0048 – – – – – – 0.0142 0.0002 0.0016 0.0004 0.0055 0.0061
None 0.0024 0.0002 0.0037 0.0004 0.0117 0.0006 – – – – – – 0.0041 0.0001 0.0003 0.0002 0.0044 0.0004
Nuclear Periphery 0.0267 0.004 0.0062 0.0065 0.0158 0.011 – – – – – – 0.0039 0.0005 0.0025 0.0039 0.0024 0.002
Nucleolus 0.0007 0 0.0024 0.0001 0.0018 0.0001 – – – – – – 0.0002 0 0 0 0.0004 0
Nucleus 0.0058 0.002 0.004 0.0034 0.0129 0.0033 – – – – – – 0.002 0.0004 0.0011 0.0013 0.0018 0.0009
Peroxisomes 0.0092 0 0.0073 0.0288 0.0002 0.0001 – – – – – – 0.0023 0 0.0012 0.0001 0.003 0.0001
Punctate Nuclear 0.0029 0 0.0016 0.0003 0.0252 0.0002 – – – – – – 0.001 0 0.0001 0.0001 0.0065 0
Vacuole 0.0802 0.0302 0.0662 0.0908 0.1191 0.0429 – – – – – – 0.0207 0.0049 0.0213 0.0391 0.0179 0.0061
Vacuole Periphery 0.0211 0.0039 0.0102 0.0171 0.0396 0.0121 – – – – – – 0.0043 0.0006 0.0049 0.0092 0.0047 0.0034

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 166.4945 138.5035 160.6439 171.3563 138.7082 139.5854 143.9584 140.9797 145.2744 154.385
Translational Efficiency 0.7347 0.7553 0.6737 0.6483 0.6319 0.7027 0.649 0.4988 0.589 0.5624

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1508 1418 213 1081 1775 1587 707 19 3283 3005 920 1100

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1558.82 2396.58 3475.92 2544.14 2161.45 2237.04 2771.55 4204.76 1884.64 2312.32 2934.63 2572.82
Standard Deviation 362.33 483.84 550.53 519.46 380.21 431.75 441.49 753.65 478.17 463.96 555.18 567.27
Intensity Change Log 2 — 0.620523 1.156941 0.706724 — 0.049592 0.358694 0.960024 — 0.316732 0.747865 0.859245

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000163 0.000122 0.000304 0.000277 0.000059 0.000094 0.000218 0.000021 0.000107 0.000107 0.000238 0.000273
Bud Neck 0.000302 0.000412 0.011494 0.002089 0.000164 0.000195 0.002827 0.001437 0.000227 0.000297 0.004833 0.002078
Bud Site 0.024247 0.008160 0.008781 0.017402 0.003602 0.005462 0.008686 0.013744 0.013085 0.006735 0.008708 0.017339
Cell Periphery 0.003398 0.003043 0.006941 0.008810 0.001701 0.001635 0.004133 0.000502 0.002480 0.002299 0.004783 0.008666
Cytoplasm 0.639817* 0.833526* 0.306186* 0.605304* 0.865866* 0.894575* 0.760838* 0.828178* 0.762034* 0.865767* 0.655577* 0.609154*
Cytoplasmic Foci 0.097331 0.026760 0.032403 0.014064 0.017581 0.015248 0.016871 0.008657 0.054213 0.020680 0.020467 0.013971
Eisosomes 0.000003 0.000001 0.000007 0.000004 0.000001 0.000001 0.000002 0.000001 0.000002 0.000001 0.000003 0.000004
Endoplasmic Reticulum 0.010239 0.001563 0.000521 0.000551 0.001865 0.000864 0.000516 0.000086 0.005712 0.001194 0.000517 0.000543
Endosome 0.005061 0.002492 0.006586 0.006043 0.001568 0.001382 0.002471 0.000791 0.003173 0.001906 0.003423 0.005952
Golgi 0.062717 0.053069 0.068994 0.028892 0.043940 0.033994 0.048160 0.001772 0.052565 0.042995 0.052984 0.028424
Lipid Particles 0.001939 0.005000 0.013355 0.011947 0.002757 0.002418 0.009955 0.000465 0.002381 0.003636 0.010743 0.011749
Mitochondria 0.001317 0.000173 0.000170 0.000314 0.000121 0.001022 0.000106 0.000285 0.000670 0.000622 0.000120 0.000314
Mitotic Spindle 0.002580 0.002643 0.007000 0.004938 0.001368 0.000724 0.006650 0.000016 0.001925 0.001629 0.006731 0.004853
None 0.000777 0.000602 0.000584 0.000420 0.000587 0.000574 0.000845 0.000076 0.000674 0.000588 0.000785 0.000414
Nuclear Periphery 0.000122 0.000120 0.002389 0.000205 0.000101 0.000049 0.000516 0.000005 0.000110 0.000082 0.000950 0.000202
Nuclear Periphery Foci 0.001975 0.007552 0.019747 0.004616 0.007054 0.003804 0.013862 0.000024 0.004721 0.005572 0.015225 0.004537
Nucleolus 0.000228 0.000034 0.000251 0.000267 0.000020 0.000043 0.000071 0.000324 0.000116 0.000039 0.000113 0.000268
Nucleus 0.002057 0.001850 0.016589 0.006817 0.001205 0.001669 0.011210 0.003380 0.001596 0.001754 0.012456 0.006758
Peroxisomes 0.000199 0.000063 0.000088 0.000453 0.000029 0.000046 0.000027 0.000501 0.000107 0.000054 0.000041 0.000453
Vacuole 0.025760 0.018965 0.416598* 0.241133* 0.014745 0.012639 0.080777 0.124037 0.019804 0.015624 0.158527 0.239110*
Vacuole Periphery 0.119768* 0.033852 0.081012 0.045454 0.035666 0.023562 0.031256 0.015697 0.074297 0.028418 0.042776 0.044940

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.90 -4.15 -3.58 -4.93 1.43 -2.46 -9.27 4.77 5.43 11.31 -0.06 -8.43 -5.58 -5.53 -0.07
Bud Neck -1.70 -9.38 -11.38 -10.68 7.80 -1.13 -9.15 -2.26 -2.20 3.29 -2.09 -12.29 -11.95 -11.55 6.78
Bud Site 10.29 7.66 3.12 -6.66 -4.49 -2.11 -6.49 -1.59 -1.29 -0.57 7.25 2.90 -3.30 -7.93 -5.21
Cell Periphery 0.88 -3.86 -4.21 -4.61 -0.45 0.31 -7.71 3.43 3.13 8.52 0.81 -6.90 -5.22 -5.41 -2.63
Cytoplasm -18.11 14.66 3.10 19.94 -12.69 -3.96 10.00 1.01 1.80 -2.20 -15.65 9.50 14.52 25.14 2.54
Cytoplasmic Foci 18.22 10.33 22.08 6.56 5.83 1.89 -0.95 4.12 3.05 4.43 16.53 13.12 18.41 4.53 5.34
Eisosomes 5.37 -5.15 -0.96 -5.97 4.52 -1.89 -9.00 -2.54 -1.08 3.71 3.80 -6.20 -4.27 -6.27 0.24
Endoplasmic Reticulum 6.76 7.66 7.65 5.82 -0.16 4.05 6.85 9.38 4.69 6.06 7.42 8.69 8.68 5.15 0.17
Endosome 6.91 -1.31 -0.52 -3.56 0.73 1.04 -4.93 3.31 2.38 6.62 6.34 -1.21 -2.78 -4.35 -2.20
Golgi 2.52 0.06 11.39 8.30 7.03 3.19 -0.92 18.22 15.06 14.78* 3.91 0.51 10.69 6.99 7.93
Lipid Particles -5.20 -8.19 -10.68 -6.59 1.51 0.69 -7.62 5.52 5.56 10.34 -3.33 -10.75 -10.15 -8.60 -0.20
Mitochondria 6.01 5.77 5.19 -1.68 -1.09 -1.73 0.35 -2.41 1.40 -2.50 0.17 6.03 3.62 1.20 -2.35
Mitotic Spindle -0.07 -3.96 -1.56 -1.43 2.06 2.74 -4.38 5.97 10.07 5.33 0.82 -5.05 -2.14 -2.37 2.17
None 1.68 1.01 3.19 3.28 2.23 0.21 -3.91 10.03 10.28 12.66 1.54 -1.80 3.73 3.29 5.87
Nuclear Periphery -0.01 -7.28 -1.84 -2.98 7.05 3.90 -7.44 8.17 6.53 9.09 1.22 -9.47 -3.00 -4.62 8.37
Nuclear Periphery Foci -7.65 -10.40 -6.82 3.48 8.58 4.24 -6.01 11.48 8.17 13.58 -1.61 -11.25 0.03 1.59 11.15
Nucleolus 1.44 -0.06 0.01 -10.95 0.35 -1.40 -12.11 -4.69 -4.18 -3.74 1.27 0.12 -1.74 -10.63 -6.95
Nucleus 0.50 -5.80 -6.54 -7.29 3.47 -1.93 -10.36 -3.23 -2.34 7.31 -0.56 -11.04 -7.41 -7.37 3.09
Peroxisomes 1.28 1.14 -0.06 -2.52 -2.27 -1.49 -0.41 -4.40 -4.23 -4.38 1.19 1.26 -1.21 -2.67 -2.73
Vacuole 3.13 -19.47 -29.03 -30.02 7.79 1.50 -13.26 -3.19 -3.25 -1.02 3.31 -18.54 -30.33 -30.91 -7.79
Vacuole Periphery 16.48* 6.21 15.26 -1.89 4.32 4.38 1.70 4.57 1.83 3.15 15.71 9.81 10.18 -4.12 -0.06
Standard name
Human Ortholog
Description Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Gcn1

Gcn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gcn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available