Standard name
Human Ortholog
Description Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain; MDS3 has a paralog, PMD1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0.08 0 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0 – 0.22 0.19 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.97 0.97 0.98 0.97 0.93 0.9 0.85 0.88 0.76 0.7 0.99 1.0 0.99 – 0.94 0.96 0.94 0.92 0.87 0.95 0.96 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.09 0 0.16 0.2 0.27 0.34 0.36 0.38 0.49 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 – 1 0 0 3 1 0 1 3
Bud 0 2 4 1 5 3 2 10 9 1 2 4 0 0 13 – 1 0 0 0 0 0 0 3
Bud Neck 0 0 0 0 0 0 1 3 1 0 0 1 0 0 0 – 0 0 4 3 3 0 1 2
Bud Site 0 0 0 0 0 1 1 2 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 28 6 2 2 4 7 5 6 4 8 7 1 5 16 9 – 26 19 0 1 0 0 0 0
Cytoplasm 345 367 160 119 253 249 290 295 180 220 89 119 194 309 341 – 110 97 326 354 144 132 263 284
Endoplasmic Reticulum 1 8 2 0 1 0 0 1 0 2 0 1 1 0 0 – 4 2 7 4 2 0 1 4
Endosome 1 0 0 0 0 0 0 0 1 0 1 0 0 0 0 – 0 0 1 4 2 0 0 1
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 2 1 0 1 0 1
Mitochondria 1 3 6 11 11 40 64 87 73 90 44 83 0 1 7 – 3 3 1 3 5 0 0 5
Nucleus 0 2 2 1 1 0 1 1 1 2 0 0 2 1 0 – 0 2 0 0 0 0 0 0
Nuclear Periphery 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 1
Nucleolus 0 0 0 1 0 0 0 1 0 1 1 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 2 1 2 2 1 1 1 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 0 0 1 0 0 0 1 0 0 0 1 0 0 0 – 0 2 0 2 1 0 0 1
Unique Cell Count 350 380 165 123 258 257 313 328 212 251 117 169 195 310 344 117 101 349 386 166 140 275 314
Labelled Cell Count 379 390 177 136 275 302 365 409 271 325 146 211 202 327 370 145 125 349 386 166 140 275 314


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 4.6 4.2 4.6 4.4 3.7 3.6 3.8 3.6 3.3 3.6 3.5 5.4 5.6 4.9 – 7.6 8.1 5.7 5.0 6.0
Std Deviation (1e-4) 0.9 0.7 1.2 2.5 1.8 1.6 1.4 1.7 2.4 1.3 2.0 1.7 0.9 1.0 1.1 – 1.6 1.9 1.6 1.2 1.5
Intensity Change (Log2) – – – 0.13 0.07 -0.16 -0.2 -0.11 -0.2 -0.35 -0.2 -0.27 0.38 0.42 0.23 – 0.87 0.96 0.46 0.26 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5701 1.1928 0.8735 0.5285 1.3033 1.2299 2.0674 2.5974 2.2021 1.8055 2.066 2.3255 1.0458 1.5463 1.2275 1.1526 1.262 1.1465
Actin 0.0514 0.0015 0.01 0.0004 0.103 0.0005 0.0603 0.0004 0.0043 0.0111 0.0124 0.0089 0.0413 0.0007 0.0014 0.0014 0.0218 0.0102
Bud 0.0034 0.0002 0.0002 0.0001 0.0013 0.0029 0.0018 0.0032 0.0013 0.0018 0.0002 0.0035 0.001 0.0003 0.0023 0.0023 0.0005 0.0003
Bud Neck 0.0018 0.0008 0.0007 0.0004 0.0029 0.0011 0.0197 0.0003 0.0005 0.0012 0.0002 0.0025 0.0004 0.0007 0.0028 0.0003 0.0005 0.0066
Bud Periphery 0.0073 0.0006 0.0004 0.0002 0.0028 0.0024 0.0036 0.0011 0.004 0.0023 0.0003 0.0055 0.0017 0.0004 0.0044 0.0044 0.0008 0.0005
Bud Site 0.0052 0.0202 0.0021 0.0003 0.0058 0.0002 0.0257 0.0101 0.0079 0.0102 0.0003 0.0026 0.0098 0.0319 0.0063 0.0011 0.0009 0.0005
Cell Periphery 0.0039 0.0071 0.004 0.0008 0.002 0.001 0.0014 0.0006 0.001 0.0003 0.0001 0.0002 0.0044 0.0021 0.002 0.0009 0.0005 0.0006
Cytoplasm 0.5452 0.7997 0.6651 0.6243 0.5958 0.7487 0.399 0.7067 0.6713 0.6432 0.6167 0.6985 0.3859 0.6336 0.5243 0.5286 0.5498 0.4821
Cytoplasmic Foci 0.0329 0.003 0.0119 0.0028 0.003 0.0029 0.0365 0.0049 0.0113 0.0109 0.0074 0.0045 0.0184 0.0029 0.0256 0.0058 0.0105 0.0071
Eisosomes 0.0012 0.0004 0.0014 0.0002 0.0009 0.0001 0.0009 0 0.0002 0.0001 0.0001 0.0001 0.0004 0.0003 0.0003 0.0003 0.0001 0.0002
Endoplasmic Reticulum 0.0082 0.0056 0.0042 0.0016 0.0081 0.0031 0.0052 0.0017 0.0021 0.0023 0.002 0.0017 0.0096 0.0012 0.0024 0.0017 0.0027 0.0014
Endosome 0.0231 0.0008 0.003 0.0008 0.0289 0.0006 0.0361 0.0012 0.0074 0.0097 0.0066 0.0105 0.0222 0.0004 0.0048 0.0016 0.004 0.0013
Golgi 0.0118 0.0003 0.0014 0.0002 0.0247 0.0001 0.0189 0.0002 0.0017 0.0048 0.0157 0.0062 0.0076 0.0001 0.002 0.0003 0.0011 0.001
Lipid Particles 0.0085 0.0005 0.006 0.0002 0.0025 0.0002 0.0176 0.0002 0.0035 0.0006 0.0363 0.0011 0.0075 0.0001 0.0012 0.0002 0.0008 0.0011
Mitochondria 0.0056 0.0001 0.0116 0.0002 0.0104 0.0001 0.0095 0.0002 0.0011 0.0017 0.025 0.0074 0.0044 0.0001 0.0004 0.0003 0.0003 0.0004
None 0.2551 0.1564 0.2568 0.3654 0.1999 0.2342 0.3036 0.2647 0.2721 0.2933 0.2504 0.2402 0.4658 0.3232 0.4108 0.447 0.4013 0.4824
Nuclear Periphery 0.0028 0.0003 0.0003 0.0002 0.0024 0.0003 0.0157 0.0002 0.0004 0.0006 0.0003 0.0023 0.0005 0.0001 0.0003 0.0003 0.0005 0.0002
Nucleolus 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001 0.002 0.0003 0.0006 0.0001 0 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002
Nucleus 0.0011 0.0005 0.0005 0.0004 0.0005 0.0005 0.0033 0.0014 0.0007 0.0007 0.0004 0.0007 0.0003 0.0005 0.0007 0.0006 0.0006 0.0003
Peroxisomes 0.0182 0 0.0177 0.0001 0.0006 0 0.0107 0.0001 0.0037 0.0017 0.0238 0.0014 0.0159 0 0.0047 0.0001 0.0007 0.0023
Punctate Nuclear 0.0071 0.0002 0.0012 0.0002 0.0003 0.0002 0.0225 0.001 0.0008 0.0005 0.0004 0.0005 0.0005 0.0002 0.0015 0.0002 0.0005 0.0004
Vacuole 0.0044 0.0014 0.0011 0.0009 0.0026 0.0009 0.0032 0.0015 0.0037 0.0025 0.0009 0.0012 0.002 0.0008 0.0015 0.0019 0.0017 0.0007
Vacuole Periphery 0.0012 0 0.0001 0.0001 0.0017 0.0001 0.0027 0.0001 0.0002 0.0003 0.0003 0.0005 0.0003 0 0.0001 0.0002 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.5943 19.6657 21.9711 30.6898 23.2346 27.9157 29.9934 30.158 35.1445 33.6153
Translational Efficiency 0.4131 0.4011 0.4063 0.357 0.3691 0.4286 0.3096 0.3499 0.3138 0.342

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
100 1027 164 1344 1668 1842 200 104 1768 2869 364 1448

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 767.61 707.44 1271.95 936.50 724.98 769.42 1051.77 970.54 727.39 747.23 1150.97 938.94
Standard Deviation 108.51 103.95 152.55 117.66 252.49 97.60 97.20 117.22 246.79 104.25 166.37 117.95
Intensity Change Log 2 — -0.117766 0.728597 0.286905 — 0.085830 0.536806 0.420846 — -0.015285 0.638618 0.353517

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000646 0.000707 0.000394 0.000702 0.000254 0.000481 0.000258 0.000492 0.000276 0.000562 0.000319 0.000687
Bud Neck 0.026641 0.029647 0.002101 0.005875 0.013596 0.032743 0.001222 0.001252 0.014333 0.031635 0.001618 0.005543
Bud Site 0.013018 0.030372 0.015375 0.040915 0.008652 0.029478 0.008192 0.030852 0.008899 0.029798 0.011428 0.040192
Cell Periphery 0.001325 0.000153 0.000271 0.000125 0.000188 0.000198 0.000197 0.000145 0.000252 0.000182 0.000231 0.000126
Cytoplasm 0.639963* 0.441068* 0.261800* 0.566748* 0.562368* 0.492133* 0.540712* 0.502205* 0.566757* 0.473854* 0.415049* 0.562112*
Cytoplasmic Foci 0.180886* 0.269711* 0.084917 0.044930 0.233692* 0.233213* 0.079378 0.088085 0.230705* 0.246278* 0.081873 0.048029
Eisosomes 0.001247 0.000155 0.000121 0.000047 0.000287 0.000223 0.000088 0.000092 0.000341 0.000199 0.000103 0.000050
Endoplasmic Reticulum 0.000999 0.000620 0.002791 0.000618 0.001159 0.000439 0.001700 0.000649 0.001150 0.000503 0.002192 0.000620
Endosome 0.010899 0.051917 0.023548 0.013192 0.018548 0.032146 0.011192 0.005611 0.018115 0.039223 0.016759 0.012647
Golgi 0.003100 0.009220 0.000227 0.006276 0.006123 0.009623 0.000172 0.000144 0.005952 0.009479 0.000197 0.005835
Lipid Particles 0.004511 0.004199 0.005359 0.000855 0.005696 0.004952 0.002521 0.001025 0.005629 0.004683 0.003800 0.000867
Mitochondria 0.000354 0.004210 0.000046 0.003071 0.002624 0.005326 0.000029 0.000015 0.002496 0.004926 0.000037 0.002851
Mitotic Spindle 0.000069 0.001924 0.000236 0.035772 0.000830 0.009094 0.000158 0.000316 0.000787 0.006527 0.000193 0.033226
None 0.000348 0.002267 0.000114 0.001743 0.002250 0.000767 0.000648 0.000344 0.002142 0.001304 0.000408 0.001642
Nuclear Periphery 0.000087 0.000204 0.000577 0.000234 0.000245 0.000095 0.000404 0.000162 0.000236 0.000134 0.000482 0.000229
Nuclear Periphery Foci 0.000673 0.000524 0.015620 0.001255 0.000692 0.000311 0.007216 0.003360 0.000691 0.000387 0.011002 0.001406
Nucleolus 0.000156 0.001152 0.000169 0.000299 0.000508 0.000471 0.000102 0.000049 0.000488 0.000715 0.000132 0.000281
Nucleus 0.037400 0.025134 0.028380 0.033499 0.055495 0.031099 0.038674 0.015252 0.054472 0.028964 0.034036 0.032189
Peroxisomes 0.001876 0.015172 0.000577 0.002462 0.003142 0.008081 0.000323 0.000556 0.003071 0.010620 0.000437 0.002326
Vacuole 0.075440 0.108687 0.557009* 0.236643* 0.082492 0.106932 0.306617* 0.349272* 0.082093 0.107560 0.419431* 0.244733*
Vacuole Periphery 0.000359 0.002957 0.000368 0.004739 0.001158 0.002196 0.000197 0.000124 0.001113 0.002468 0.000274 0.004407

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.14 0.84 0.79 1.57 -0.05 -2.96 -0.64 -2.58 -0.20 -2.34 -3.55 -1.55 -1.83 1.19 -1.09
Bud Neck -0.37 3.31 3.10 11.40 -3.19 -9.81 13.01 13.14 18.45 0.79 -10.28 12.85 10.26 19.87 -4.18
Bud Site -6.43 -0.79 -3.87 1.92 -2.83 -10.46 0.27 -1.88 1.38 -1.91 -13.46 -1.99 -6.41 2.23 -4.93
Cell Periphery 4.03 3.73 4.27 3.19 8.72 -0.21 -0.07 4.81 1.58 3.76 1.91 1.90 8.36 2.97 10.03
Cytoplasm 9.07 13.82 5.20 -6.93 -12.94 8.05 3.83 3.70 1.35 0.88 11.98 11.48 4.56 -4.92 -8.10
Cytoplasmic Foci -6.01 7.69 11.08 33.52 5.88 0.17 23.80 20.89 20.56 0.76 -2.54 27.44 41.77 49.91 8.30
Eisosomes 3.12 3.27 3.45 10.90 8.61 3.25 11.28 11.52 10.28 1.69 5.36 9.48 11.83 18.62 11.07
Endoplasmic Reticulum 1.08 -3.36 1.65 1.52 8.71 3.96 -1.49 3.02 -1.69 3.59 3.71 -3.26 3.97 0.84 7.76
Endosome -9.22 -1.86 1.38 14.87 6.08 -7.37 5.68 11.93 17.60 5.39 -11.90 3.24 9.15 19.98 5.96
Golgi -4.49 3.12 0.51 5.59 -3.40 -3.43 9.16 9.22 12.28 1.97 -4.01 9.35 4.12 8.14 -3.44
Lipid Particles 0.41 0.28 4.46 6.82 7.38 1.11 6.76 13.75 6.54 3.31 1.86 5.64 15.16 9.06 7.69
Mitochondria -5.89 4.28 -2.49 3.71 -3.33 -3.40 5.17 5.18 8.69 1.01 -3.64 5.20 2.07 6.24 -3.33
Mitotic Spindle -2.53 -4.82 -6.88 -5.94 -6.76 -4.86 1.93 0.17 4.94 -2.46 -5.01 1.64 -6.55 -3.88 -6.80
None -2.36 3.16 -3.70 0.90 -4.44 2.72 3.09 3.09 1.47 -0.09 1.42 3.48 1.24 -0.28 -3.63
Nuclear Periphery -2.34 -10.90 -12.87 -3.19 4.71 1.11 -1.65 -0.04 -4.55 4.06 0.79 -2.22 -0.94 -9.25 4.79
Nuclear Periphery Foci 0.89 -9.97 -2.38 -3.85 9.58 1.85 -9.10 -4.61 -5.99 5.61 1.56 -12.79 -2.60 -6.02 12.12
Nucleolus -2.81 0.91 -0.95 2.69 -1.86 0.55 7.15 8.04 8.26 5.06 -1.53 6.97 4.93 3.97 -2.24
Nucleus 2.60 2.29 -0.18 -5.64 -4.27 11.32 2.88 7.23 1.86 3.35 12.91 6.32 7.55 -4.69 -0.77
Peroxisomes -8.83 4.16 1.50 9.25 -1.59 -7.38 9.45 8.60 12.60 -2.47 -10.57 9.41 4.43 12.84 -1.91
Vacuole -3.08 -22.63 -19.37 -24.54 11.59 -6.37 -15.05 -11.77 -10.88 -1.68 -7.76 -25.71 -32.03 -28.55 7.34
Vacuole Periphery -7.41 1.39 -2.75 1.47 -2.88 -4.53 11.53 13.04 9.91 2.65 -6.72 11.22 -1.28 1.15 -2.97
Standard name
Human Ortholog
Description Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain; MDS3 has a paralog, PMD1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (88%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mds3

Mds3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mds3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available