Standard name
Human Ortholog
Description Aromatic aminotransferase I; expression is regulated by general control of amino acid biosynthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0 0.05 0.05 0.08 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 1.0 0.99 0.99 0.98 0.93 0.89 0.9 0.89 0.86 0.86 1.0 0.99 0.99 0.99 0.98 0.99 0.97 0.97 0.96 0.94 0.95 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.08 0.06 0.08 0.08 0.05 0 0 0 0 0.07 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 4 7 3 9 0 0 0 0 0 0 0 0 0 1 0 1
Bud 3 2 1 0 0 1 1 2 0 1 0 1 0 0 0 2 0 0 3 1 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 5 3 1 3 19 13 13 15 18 29 5 4 5 5 6 3 0 0 0 0 0 0
Cytoplasm 277 337 204 203 241 305 358 321 249 276 199 239 207 334 427 228 187 188 252 328 183 108 155 180
Endoplasmic Reticulum 0 1 0 2 2 2 18 29 16 25 18 15 1 2 3 5 13 16 0 1 2 1 0 4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Golgi 0 0 0 0 0 0 0 1 2 0 2 1 0 0 0 0 0 0 0 0 0 0 0 2
Mitochondria 0 0 0 0 0 0 1 0 5 2 3 7 0 0 0 0 1 0 0 0 0 1 0 1
Nucleus 0 0 0 0 1 1 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 0 0 0 0 0 1 0 1 1 2 1 0 0 0 4 2 0 1 1 0 1 3
Unique Cell Count 277 342 204 205 243 311 385 360 276 309 231 277 208 336 432 231 191 189 261 339 192 116 164 206
Labelled Cell Count 280 345 210 208 245 312 398 370 289 327 245 304 214 340 436 241 211 209 261 339 192 116 164 206


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 121.0 133.5 123.9 118.1 109.7 86.4 74.4 62.3 57.9 53.6 49.8 46.8 111.4 111.0 107.7 111.5 127.1 134.9 89.6 98.1 101.2
Std Deviation (1e-4) 28.8 30.6 31.2 28.7 21.5 22.9 18.8 18.1 16.3 14.4 13.8 13.9 23.1 25.5 22.4 28.8 45.3 36.4 29.3 26.7 36.4
Intensity Change (Log2) – – – -0.07 -0.18 -0.52 -0.74 -0.99 -1.1 -1.21 -1.32 -1.41 -0.15 -0.16 -0.2 -0.15 0.04 0.12 -0.47 -0.34 -0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1.5 0.8 1.3 1.4 2.5 3.4 0 0 0 0 0.4 0
Cytoplasm -1.4 -1.3 -2.0 -3.9 -4.9 -4.6 -4.8 -5.5 -5.5 -1.0 -1.1 -1.5 -1.6 -2.1 -1.0
Endoplasmic Reticulum 0 0 0 3.1 4.2 3.5 4.2 4.1 3.4 0 0 0 0 3.8 4.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 86.4671 111.1547 96.209 92.4528 90.5761 91.499 85.9906 112.8638 102.3994 99.7049 88.3674 103.8023 94.1842 115.6322 106.0941 96.3593 103.1707 105.7636
Actin 0.0122 0.0001 0.0083 0.0001 0.0003 0.0014 0.0279 0.0002 0.0043 0.0001 0.0008 0.0001 0.0071 0.0001 0.0002 0.0034 0.0003 0.0005
Bud 0.0016 0 0.0001 0.0004 0 0.0011 0.0007 0 0.0006 0.0001 0.0089 0.0001 0.0008 0 0.0002 0.0001 0.0001 0.0003
Bud Neck 0.0007 0.0001 0.0029 0.0004 0.0003 0.0021 0.0057 0.0002 0.0002 0.0002 0.0009 0.0004 0.0003 0.0001 0.0002 0.0002 0.0002 0.0008
Bud Periphery 0.0011 0 0.0001 0.0001 0 0.0019 0.0007 0 0.0008 0.0001 0.0046 0 0.0007 0 0.0002 0.0002 0 0.0001
Bud Site 0.0008 0 0.0021 0 0.0001 0.0029 0.002 0 0.0005 0 0.0009 0 0.0007 0 0.0002 0.0003 0.0001 0
Cell Periphery 0.003 0.0002 0.0003 0.0001 0.0001 0.0013 0.0017 0 0.0005 0.0003 0.0012 0 0.0003 0.0001 0.0007 0.0007 0.0001 0.0001
Cytoplasm 0.9165 0.997 0.9606 0.9819 0.9108 0.8882 0.8769 0.9976 0.9659 0.9641 0.9057 0.9972 0.9474 0.9982 0.9815 0.9237 0.9471 0.9938
Cytoplasmic Foci 0.0058 0.0001 0.0029 0.0004 0.0201 0.0072 0.0117 0.0001 0.0049 0.004 0.0073 0.0003 0.0051 0.0001 0.0017 0.0051 0.0274 0.0005
Eisosomes 0.0003 0 0.0002 0 0 0.0001 0.0005 0 0.0001 0.0001 0 0 0.0001 0 0 0.0005 0.0001 0
Endoplasmic Reticulum 0.0033 0.0002 0.0003 0.0002 0.0004 0.005 0.0033 0.0001 0.0018 0.0001 0.0003 0.0001 0.0009 0.0001 0.0001 0.001 0.0003 0.0005
Endosome 0.0043 0 0.0003 0.0002 0.0008 0.0017 0.0066 0 0.0009 0.0001 0.0019 0 0.0031 0 0.0001 0.0048 0.0008 0.0001
Golgi 0.0007 0 0.0007 0 0.001 0.0004 0.0027 0 0.0003 0 0.0003 0 0.0013 0 0.0001 0.0014 0.0003 0
Lipid Particles 0.0015 0 0.0009 0 0.0133 0.0035 0.0041 0 0.0014 0.0012 0.0026 0 0.0046 0 0.0006 0.0103 0.0057 0
Mitochondria 0.0042 0 0.0015 0.0002 0.0027 0.008 0.0048 0 0.0053 0.0136 0.0085 0 0.0026 0 0.0053 0.0068 0.0018 0.0001
None 0.0054 0 0.0025 0.0004 0.0016 0.0017 0.0112 0.0001 0.0035 0.0022 0.0056 0.0001 0.0035 0.0001 0.0031 0.0049 0.0044 0.0002
Nuclear Periphery 0.0056 0.0005 0.0013 0.001 0.0016 0.0042 0.0099 0.0005 0.0011 0.0003 0.0016 0.0003 0.0025 0.0002 0.0003 0.0046 0.0007 0.0006
Nucleolus 0.0054 0 0.0066 0.0003 0.0014 0.0265 0.0054 0 0.0018 0.0079 0.0077 0 0.0031 0 0.0008 0.0161 0.0029 0
Nucleus 0.0161 0.0013 0.0042 0.0046 0.0039 0.0297 0.0075 0.0009 0.0022 0.002 0.0055 0.0012 0.0076 0.0009 0.0017 0.0073 0.0021 0.0021
Peroxisomes 0.0003 0 0.0009 0 0.0376 0.0006 0.0014 0 0.0003 0.0008 0.0006 0 0.0011 0 0.0015 0.002 0.0007 0
Punctate Nuclear 0.0008 0 0.0004 0.0001 0.0025 0.002 0.002 0 0.0003 0.0001 0.0003 0 0.0013 0 0.0001 0.0028 0.0037 0
Vacuole 0.0078 0.0003 0.0017 0.0072 0.0011 0.0073 0.0098 0.0002 0.0022 0.0013 0.017 0.0001 0.0038 0.0002 0.0012 0.0015 0.0006 0.0003
Vacuole Periphery 0.0025 0 0.001 0.0025 0.0006 0.0029 0.0035 0 0.0011 0.0014 0.0179 0 0.0019 0 0.0002 0.0022 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 315.6779 301.2287 309.2335 310.5516 285.5513 321.373 301.2542 305.2134 300.1694 293.5076
Translational Efficiency 1.3341 1.3618 1.245 1.2814 1.3693 1.3089 1.3872 1.1208 1.1708 1.1678

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1704 923 192 1329 384 1782 883 63 2088 2705 1075 1392

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 4980.88 6551.78 4783.55 4482.12 6220.43 8291.65 5297.14 4507.77 5208.84 7697.97 5205.41 4483.28
Standard Deviation 1116.44 1744.74 1073.66 985.98 1205.05 1995.26 968.86 1105.37 1230.80 2083.71 1007.78 991.70
Intensity Change Log 2 — 0.395486 -0.058319 -0.152219 — 0.414645 -0.231801 -0.464600 — 0.406157 -0.152073 -0.317292

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000063 0.000062 0.000169 0.000585 0.000075 0.000054 0.000195 0.003210 0.000065 0.000057 0.000191 0.000704
Bud Neck 0.000217 0.000337 0.003837 0.001815 0.000829 0.000352 0.001189 0.002164 0.000330 0.000347 0.001662 0.001831
Bud Site 0.004299 0.001258 0.001382 0.010869 0.001712 0.001028 0.001842 0.009257 0.003823 0.001106 0.001760 0.010796
Cell Periphery 0.002957 0.002467 0.008168 0.003518 0.011214 0.001178 0.001672 0.002213 0.004476 0.001618 0.002833 0.003459
Cytoplasm 0.918407* 0.897234* 0.727586* 0.853796* 0.833231* 0.888145* 0.839077* 0.680290* 0.902742* 0.891246* 0.819164* 0.845943*
Cytoplasmic Foci 0.008295 0.003468 0.023518 0.006153 0.013113 0.005619 0.008053 0.036398 0.009181 0.004885 0.010815 0.007522
Eisosomes 0.000001 0.000001 0.000005 0.000005 0.000002 0.000001 0.000002 0.000019 0.000001 0.000001 0.000002 0.000006
Endoplasmic Reticulum 0.000128 0.000023 0.000040 0.000071 0.000041 0.000060 0.000025 0.001228 0.000112 0.000047 0.000027 0.000123
Endosome 0.000184 0.000041 0.000262 0.001152 0.000153 0.000081 0.000182 0.016777 0.000178 0.000068 0.000197 0.001859
Golgi 0.012258 0.002132 0.005849 0.002138 0.013261 0.002066 0.003065 0.018418 0.012442 0.002088 0.003562 0.002875
Lipid Particles 0.001659 0.002102 0.010709 0.007643 0.004775 0.001153 0.002443 0.014324 0.002232 0.001477 0.003919 0.007945
Mitochondria 0.000273 0.000059 0.000098 0.001487 0.000107 0.000146 0.000552 0.004018 0.000243 0.000116 0.000471 0.001601
Mitotic Spindle 0.001092 0.000186 0.000578 0.001681 0.000548 0.001085 0.001249 0.044350 0.000992 0.000778 0.001129 0.003612
None 0.004936 0.014181 0.003108 0.003790 0.008423 0.009804 0.004568 0.002376 0.005577 0.011298 0.004308 0.003726
Nuclear Periphery 0.000117 0.000101 0.000884 0.000133 0.000558 0.000136 0.000255 0.000683 0.000198 0.000124 0.000368 0.000158
Nuclear Periphery Foci 0.002289 0.002171 0.006984 0.002058 0.004971 0.001488 0.002504 0.004970 0.002782 0.001721 0.003304 0.002189
Nucleolus 0.000065 0.000073 0.000093 0.000105 0.000088 0.000107 0.000075 0.000076 0.000070 0.000095 0.000078 0.000103
Nucleus 0.020372 0.061750 0.128770 0.053974 0.073420 0.079183 0.106699 0.054386 0.030128 0.073234 0.110641 0.053993
Peroxisomes 0.000022 0.000006 0.000009 0.000102 0.000005 0.000016 0.000053 0.000608 0.000019 0.000013 0.000045 0.000125
Vacuole 0.007024 0.011715 0.075879 0.048157 0.023156 0.007025 0.025596 0.084688 0.009991 0.008625 0.034577 0.049811
Vacuole Periphery 0.015341 0.000633 0.002073 0.000768 0.010318 0.001273 0.000702 0.019547 0.014417 0.001055 0.000947 0.001618

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.61 -6.31 -3.41 -3.30 -2.16 1.10 -1.41 -1.13 -1.15 -0.95 -0.13 -1.86 -3.58 -3.57 -1.98
Bud Neck -3.50 -5.28 -11.61 -9.63 2.72 1.36 -5.45 -4.37 -4.74 -2.79 -1.83 -10.34 -11.70 -10.13 0.48
Bud Site 4.56 4.49 -5.36 -7.81 -7.74 0.96 -1.04 -3.10 -3.34 -2.69 5.30 1.85 -5.99 -8.38 -6.75
Cell Periphery 0.62 -6.29 -1.21 -1.87 6.04 7.12 6.57 6.48 -0.77 0.62 5.75 2.33 2.03 -5.45 -0.40
Cytoplasm 5.37 14.13 17.71 12.57 -7.08 -4.68 4.27 5.44 6.88 4.21 4.62 19.37 16.34 14.17 -3.96
Cytoplasmic Foci 4.64 -6.36 -2.17 -5.53 5.29 3.49 0.11 -2.08 -2.62 -2.11 3.74 -5.46 -2.31 -4.74 2.26
Eisosomes -2.08 -6.28 -9.07 -7.76 0.93 3.06 -0.94 -1.70 -2.05 -1.59 -0.17 -7.70 -8.64 -8.50 -3.60
Endoplasmic Reticulum 6.09 4.43 3.10 -1.91 -0.38 -0.49 1.30 -1.23 -1.16 -1.26 2.42 5.52 1.67 -0.78 -1.77
Endosome 6.35 -2.97 -1.96 -2.43 -1.26 1.09 -0.93 -1.72 -1.74 -1.70 4.38 -1.70 -2.54 -2.87 -2.27
Golgi 9.41 4.64 9.25 0.94 4.68 6.66 5.96 1.54 -1.09 -0.93 11.31 8.75 10.25 0.62 2.07
Lipid Particles -1.28 -5.96 -8.26 -7.39 1.59 3.97 1.04 -2.47 -3.37 -2.75 1.60 -6.09 -8.12 -8.92 -4.40
Mitochondria 5.07 3.85 -1.40 -1.89 -1.79 -1.65 -0.96 -1.03 -0.98 -0.77 2.82 -0.38 -1.57 -1.84 -1.20
Mitotic Spindle 3.88 1.67 -0.38 -1.76 -1.13 -1.39 -1.08 -1.22 -1.16 -1.17 0.31 -0.17 -1.17 -1.25 -0.97
None -6.07 3.52 2.75 7.92 -1.53 -0.99 3.43 5.20 10.54 5.22 -6.64 3.07 4.20 12.04 2.29
Nuclear Periphery -0.04 -6.30 -2.71 -3.86 5.82 3.99 1.73 -0.36 -2.70 -1.41 1.54 -7.00 -0.63 -3.42 7.67
Nuclear Periphery Foci -0.44 -4.39 -2.68 -1.83 3.81 4.50 2.56 -0.52 -3.11 -2.05 2.84 -2.88 -1.44 -5.33 2.00
Nucleolus -1.13 -2.74 -2.63 -1.36 0.36 -0.60 -0.46 -0.72 0.30 -0.49 -1.17 -2.42 -2.69 0.08 -0.64
Nucleus -14.20 -12.61 -17.33 -0.78 7.34 -1.93 -8.48 0.04 0.86 4.15 -19.39 -24.02 -13.60 3.31 13.66
Peroxisomes 2.80 1.72 -4.34 -5.56 -5.17 -1.12 -1.54 -1.49 -1.41 -1.22 0.66 -0.95 -4.76 -4.81 -2.12
Vacuole -4.47 -8.46 -20.68 -16.15 2.08 4.67 -5.62 -5.51 -6.11 -4.50 -0.70 -13.35 -19.91 -19.70 -3.49
Vacuole Periphery 9.17 8.06 9.20 0.44 3.55 2.97 3.18 0.63 -0.90 -1.00 9.31 9.45 9.40 0.69 0.37
Standard name
Human Ortholog
Description Aromatic aminotransferase I; expression is regulated by general control of amino acid biosynthesis
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Aro8

Aro8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aro8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available