Standard name
Human Ortholog
Description Cell death protease essential for hypochlorite-induced apoptosis; involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.09 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0.08 0.06 0 0.06 0.06
Bud 0 0 0 0 0 0 0 0 0.06 0.06 0.05 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0.33 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.06 0 0.08 0.07 0.13 0.09 0.12 0.09 0.05 0.05 0 0 0.05 0 0.37 0.1 0.08 0.09 0.12 0.06 0.07
Golgi 0.34 0.25 0.26 0.2 0.35 0.13 0.13 0.08 0 0 0 0.59 0.76 0.76 0.18 0.72 0.67 0.53 0.57 0.7 0.75
Mitochondria 0.86 0.92 0.81 0.79 0.59 0.78 0.72 0.75 0.87 0.85 0.84 0.71 0.49 0.48 0.07 0 0.06 0 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Nucleolus 0.07 0.06 0.05 0.07 0.07 0.11 0.12 0.15 0.1 0.13 0.09 0 0 0 0.07 0 0 0 0 0 0
Peroxisomes 0 0 0.09 0.05 0 0 0 0.05 0 0 0.07 0 0 0 0 0.08 0.06 0.26 0.18 0.13 0.07
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0.11 0.06 0.06 0 0 0 0 0 0.13 0.12 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 26 1 3 5 2 1 11 7 9 2 4 11 4 0 6 23 21 6 17 16
Bud 2 5 5 4 2 8 11 23 30 28 33 0 0 0 0 2 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 1 0 0 0 1 1 3 8 9 10 0 0 0 0
Cell Periphery 2 1 0 2 0 0 2 1 5 2 3 1 0 0 0 0 0 0 0 0 0
Cytoplasm 6 6 7 15 18 19 14 15 8 5 5 1 1 5 19 0 0 0 0 0 0
Endoplasmic Reticulum 2 2 2 1 2 0 2 3 0 1 2 0 3 1 0 0 0 2 0 3 1
Endosome 14 4 25 20 39 39 49 55 27 21 23 3 9 5 21 26 23 30 19 15 17
Golgi 86 72 83 57 105 57 50 46 12 10 10 43 135 117 10 192 205 187 89 196 194
Mitochondria 217 264 259 221 176 342 283 446 471 390 515 52 86 74 4 8 16 11 9 5 6
Nucleus 0 0 0 0 2 1 1 3 1 0 0 0 0 0 0 0 1 0 0 0 0
Nuclear Periphery 2 3 2 0 1 8 6 5 9 7 5 0 2 4 4 0 1 1 0 0 1
Nucleolus 17 16 16 20 22 47 49 90 53 58 54 3 5 2 4 0 0 0 0 0 0
Peroxisomes 11 5 30 15 9 14 7 28 21 13 41 1 2 0 1 22 19 90 28 35 18
SpindlePole 3 1 3 4 4 5 12 16 24 20 27 0 1 0 5 2 4 2 0 1 0
Vac/Vac Membrane 15 9 8 6 33 27 25 10 9 7 16 0 23 19 6 2 6 1 1 1 1
Unique Cell Count 252 286 321 280 298 437 395 593 544 459 614 73 177 153 57 266 306 351 158 281 261
Labelled Cell Count 385 415 441 368 418 570 513 755 685 580 746 108 278 231 74 266 306 351 158 281 261


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.8 14.3 13.0 12.6 12.1 9.9 10.0 9.2 8.3 8.1 8.3 12.9 14.1 14.0 7.3 14.4 15.0 14.9
Std Deviation (1e-4) 2.5 3.3 2.5 2.3 2.1 2.0 1.9 1.9 1.8 1.8 1.8 2.6 2.3 2.4 1.8 2.4 2.5 2.4
Intensity Change (Log2) -0.05 -0.11 -0.4 -0.39 -0.51 -0.65 -0.69 -0.65 -0.01 0.12 0.11 -0.84 0.14 0.2 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.3 1.1 1.9 2.9 3.1 2.8 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.1 2.4 1.6 1.1 0.3 -0.8 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.3 2.2 0.6 2.0 0.8 -1.7 -1.9 -2.7 -1.1 -1.1 -1.9 6.2
Golgi -1.6 2.5 -4.5 -4.5 -7.5 -10.7 -10.0 -11.8 5.5 10.9 10.4 -1.3
Mitochondria -0.5 -5.9 -0.8 -2.8 -1.9 2.3 1.6 1.2 -1.8 -7.4 -7.2 -11.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.1 1.2 2.8 3.4 4.6 2.5 3.6 2.1 0 -1.1 -2.0 0
Peroxisomes -1.9 -3.2 -3.6 -4.6 -2.7 -3.3 -3.9 -1.5 -2.3 -3.6 -3.9 0
SpindlePole 0 0 0 2.0 1.8 2.8 2.8 2.9 0 0 0 0
Vacuole -0.3 4.3 2.4 2.4 -0.8 -0.9 -1.0 0.1 0 4.6 4.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.3131 11.6616 10.8062 9.8102 9.3053 10.6057 8.2609 11.8528 9.9469 8.4407 9.6069 10.3438 10.0588 14.2811 12.2065 11.6056 11.1969 11.8464
Actin 0.0297 0.0121 0.0217 0.0115 0.0038 0.0145 0.026 0.0194 0.0186 0.0059 0.008 0.0164 0.0245 0.0117 0.0223 0.0835 0.0032 0.0194
Bud 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0006 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001
Bud Neck 0.0004 0.0003 0.0005 0.0002 0.0001 0.0005 0.0003 0.0009 0.0003 0.0006 0.0001 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002
Bud Periphery 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0004 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001
Bud Site 0.0009 0.0005 0.0018 0.0002 0.0002 0.0002 0.0003 0.0018 0.0008 0.0002 0 0.0001 0.0002 0.0002 0.0002 0.0001 0 0.0001
Cell Periphery 0.0001 0.0001 0.0003 0 0.0001 0.0001 0.0001 0.0002 0.0001 0 0 0.0001 0 0.0001 0 0 0 0
Cytoplasm 0.0023 0.0008 0.0009 0.0007 0.001 0.0003 0.0008 0.0008 0.0008 0.0004 0.0001 0.0002 0.0002 0.0003 0.0002 0.0002 0.0002 0.0002
Cytoplasmic Foci 0.0703 0.0738 0.0491 0.0739 0.0646 0.0365 0.0913 0.088 0.0553 0.0952 0.0543 0.0424 0.0729 0.049 0.0502 0.0624 0.0621 0.0415
Eisosomes 0.0002 0 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0003 0 0.0001 0.0001 0.0001 0 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.002 0.0002 0.0005 0.0002 0.0004 0.0003 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001
Endosome 0.1374 0.1365 0.1345 0.1893 0.1182 0.0826 0.121 0.1032 0.0887 0.065 0.0738 0.0663 0.099 0.1052 0.0758 0.0936 0.1127 0.0635
Golgi 0.5731 0.6932 0.7 0.6651 0.672 0.7842 0.4216 0.5267 0.5559 0.6199 0.5435 0.5457 0.5381 0.6924 0.6741 0.5496 0.6784 0.712
Lipid Particles 0.0656 0.0378 0.0391 0.0267 0.0768 0.034 0.1544 0.0719 0.1226 0.1307 0.1681 0.1553 0.1501 0.0624 0.0537 0.1347 0.0942 0.0628
Mitochondria 0.0592 0.0257 0.037 0.0072 0.0354 0.0301 0.0576 0.0615 0.0813 0.004 0.078 0.0539 0.0277 0.0242 0.0377 0.0257 0.0088 0.0382
None 0.002 0 0.0003 0.0001 0.0008 0.0001 0.0005 0.0001 0.0051 0 0.0001 0 0 0 0 0 0 0
Nuclear Periphery 0.0034 0.0001 0.0002 0.0003 0.0011 0.0017 0.0002 0 0.0006 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001
Nucleolus 0.0011 0.0002 0.0001 0 0.0007 0.0004 0.0002 0.0011 0.0039 0.0001 0.0001 0.0001 0.0001 0 0 0 0 0
Nucleus 0.0006 0.0001 0.0001 0 0.0006 0.0006 0.0001 0.0002 0.0025 0 0 0 0 0 0 0 0 0
Peroxisomes 0.0365 0.0139 0.0085 0.018 0.0074 0.0079 0.1184 0.1046 0.0546 0.0768 0.0715 0.1161 0.0841 0.0492 0.0824 0.0465 0.0373 0.0596
Punctate Nuclear 0.0064 0.0001 0.0001 0.0002 0.012 0.0021 0.0018 0.0001 0.0031 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Vacuole 0.0035 0.0016 0.0019 0.0026 0.0014 0.001 0.0011 0.0148 0.0017 0.0002 0.0004 0.0006 0.0005 0.001 0.0006 0.0006 0.0009 0.0004
Vacuole Periphery 0.0047 0.0031 0.0028 0.0033 0.0033 0.0028 0.0038 0.0034 0.0032 0.0004 0.0015 0.0022 0.0016 0.0036 0.0019 0.0022 0.0016 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.9553 35.1312 31.4163 34.3111 38.5416 25.6815 38.1397 39.0352 39.3081 32.7331
Translational Efficiency 1.368 1.1533 1.3146 1.3504 0.8407 1.2123 1.0744 0.8812 0.8153 0.9276

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
571 1165 73 983 1891 1685 2363 1514 2462 2850 2436 2497

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1218.54 1168.09 1566.13 1340.78 1183.11 1204.41 1354.13 1395.90 1191.33 1189.56 1360.48 1374.20
Standard Deviation 177.94 219.33 203.26 228.77 191.46 185.26 190.11 213.19 189.00 200.69 193.92 221.10
Intensity Change Log 2 -0.061002 0.362050 0.137919 0.025742 0.194782 0.238611 -0.017618 0.282071 0.188401

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.038702 0.037259 0.013058 0.056973 0.013217 0.009866 0.045041 0.017386 0.019127 0.021063 0.044083 0.032971
Bud Neck 0.001225 0.001232 0.012244 0.002711 0.000377 0.000446 0.000482 0.001512 0.000574 0.000767 0.000834 0.001984
Bud Site 0.007325 0.002252 0.004059 0.010576 0.000543 0.000461 0.000619 0.004057 0.002116 0.001193 0.000722 0.006623
Cell Periphery 0.000646 0.000117 0.000197 0.000308 0.000098 0.000074 0.000115 0.000125 0.000225 0.000091 0.000118 0.000197
Cytoplasm 0.002149 0.002138 0.000589 0.003727 0.000677 0.000727 0.000567 0.001557 0.001018 0.001304 0.000568 0.002411
Cytoplasmic Foci 0.185256* 0.162852 0.234133* 0.116361 0.154361 0.223963* 0.113400 0.159933 0.161527 0.198982* 0.117019 0.142780
Eisosomes 0.000192 0.000423 0.000150 0.000311 0.000255 0.000177 0.000468 0.000132 0.000240 0.000278 0.000459 0.000202
Endoplasmic Reticulum 0.002600 0.000535 0.001422 0.000972 0.000362 0.000314 0.000571 0.000371 0.000881 0.000404 0.000596 0.000607
Endosome 0.163304 0.088756 0.192857 0.122437 0.074085 0.100364 0.067718 0.135106 0.094777 0.095619 0.071468 0.130119
Golgi 0.477625* 0.500405* 0.279298* 0.531761* 0.521429* 0.486564* 0.620780* 0.554669* 0.511269* 0.492222* 0.610547* 0.545651*
Lipid Particles 0.017441 0.099653 0.153850* 0.028096 0.028237 0.052034 0.081726 0.023098 0.025733 0.071499 0.083887 0.025065
Mitochondria 0.042037 0.071209 0.016697 0.073113 0.186100* 0.101166* 0.053071 0.053889 0.152688* 0.088920 0.051981 0.061457
Mitotic Spindle 0.005896 0.002183 0.014684 0.006241 0.000649 0.001011 0.000917 0.005438 0.001866 0.001490 0.001329 0.005754
None 0.000091 0.000181 0.000118 0.000443 0.000194 0.000111 0.000508 0.000105 0.000170 0.000139 0.000496 0.000238
Nuclear Periphery 0.000270 0.000141 0.003676 0.000320 0.000200 0.000265 0.000380 0.000537 0.000216 0.000214 0.000478 0.000452
Nuclear Periphery Foci 0.000835 0.001009 0.004859 0.000441 0.000306 0.000389 0.000368 0.000972 0.000429 0.000643 0.000503 0.000763
Nucleolus 0.000033 0.000129 0.000182 0.000178 0.000030 0.000058 0.000030 0.000080 0.000031 0.000087 0.000034 0.000118
Nucleus 0.000060 0.000072 0.000095 0.000059 0.000027 0.000072 0.000022 0.000051 0.000034 0.000072 0.000024 0.000054
Peroxisomes 0.013164 0.023569 0.011030 0.018281 0.006252 0.012216 0.002872 0.010779 0.007855 0.016856 0.003116 0.013732
Vacuole 0.029469 0.003274 0.041607 0.016155 0.004663 0.004459 0.003966 0.017349 0.010416 0.003975 0.005094 0.016879
Vacuole Periphery 0.011679 0.002610 0.015195 0.010537 0.007938 0.005264 0.006379 0.012855 0.008805 0.004179 0.006643 0.011942

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.22 4.35 -2.58 -3.52 -8.43 1.98 -10.42 -1.80 -3.41 8.22 -0.90 -7.96 -5.07 -4.51 3.18
Bud Neck 0.09 -4.01 -4.89 -4.72 3.45 -1.05 -2.17 -6.88 -6.25 -6.24 -2.20 -2.42 -9.35 -7.50 -6.64
Bud Site 4.16 2.64 -1.72 -6.10 -4.56 0.63 -0.49 -6.42 -6.62 -6.25 2.72 4.64 -6.75 -8.59 -9.72
Cell Periphery 1.28 1.09 0.81 -4.42 -2.39 4.06 -0.73 -3.88 -7.05 -0.39 1.39 1.08 0.28 -5.98 -2.73
Cytoplasm 0.25 2.68 -1.16 -1.44 -4.24 -0.74 1.26 -3.43 -3.26 -3.77 -0.78 2.92 -3.65 -2.56 -5.58
Cytoplasmic Foci 2.92 -2.53 9.13 8.57 6.30 -14.41 10.44 -1.16 12.20 -10.48 -9.51 11.82 4.85 14.46 -6.95
Eisosomes -8.87 1.60 -4.68 3.89 -5.66 5.98 -9.58 9.45 4.85 16.53 -2.79 -10.44 2.91 5.73 12.35
Endoplasmic Reticulum 1.67 0.93 1.31 -3.14 1.18 0.70 -4.53 -0.24 -0.83 4.18 1.63 0.99 0.94 -2.96 -0.16
Endosome 10.32 -1.38 5.39 -6.00 3.37 -6.83 2.10 -14.08 -7.15 -15.83 -0.25 7.31 -9.61 -9.36 -16.56
Golgi -1.76 6.85 -3.72 -2.57 -8.96 4.02 -11.95 -3.49 -7.28 7.52 2.63 -12.80 -4.34 -7.20 8.33
Lipid Particles -14.39 -5.37 -2.84 12.94 4.96 -7.23 -14.79 2.05 8.75 16.14 -15.05 -16.58 0.30 15.38 16.85
Mitochondria -4.11 3.34 -4.16 -0.26 -7.30 10.61 19.05 18.26 7.92 -0.17 10.19 17.10 15.26 5.96 -2.30
Mitotic Spindle 2.50 -1.58 -0.24 -3.29 1.52 -0.87 -0.62 -5.83 -5.02 -5.08 0.78 1.08 -5.64 -5.87 -6.05
None -3.02 -0.34 -1.20 -0.93 -1.15 9.33 -1.11 9.79 0.85 1.42 3.54 -1.18 -0.58 -0.88 0.88
Nuclear Periphery 2.19 -4.11 -0.75 -5.06 4.05 -0.80 -3.21 -2.94 -2.00 -1.29 0.05 -4.32 -3.23 -2.86 0.28
Nuclear Periphery Foci -0.25 -2.66 1.73 0.94 2.96 -0.59 -0.68 -1.96 -1.60 -1.78 -0.80 -0.65 -1.54 -0.37 -1.22
Nucleolus -1.80 -2.45 -1.79 -0.52 0.02 -1.38 0.02 -2.61 -0.85 -2.48 -2.27 -0.35 -2.61 -0.78 -2.46
Nucleus 0.22 -0.07 0.62 0.62 1.61 -1.23 0.70 -1.65 0.79 -2.05 -1.29 1.40 -0.89 0.95 -2.96
Peroxisomes -3.50 0.61 -1.87 1.92 -2.22 -4.44 6.03 -4.01 0.89 -7.73 -6.95 7.02 -5.23 2.12 -11.16
Vacuole 7.73 -1.36 3.73 -8.75 3.19 -0.07 1.24 -10.04 -9.13 -11.02 6.28 5.56 -5.09 -12.53 -12.06
Vacuole Periphery 6.44 -0.98 0.70 -8.33 1.37 3.31 2.49 -5.19 -7.83 -7.86 7.14 3.54 -3.97 -11.18 -8.04
Standard name
Human Ortholog
Description Cell death protease essential for hypochlorite-induced apoptosis; involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
Localization
Cell Percentages
Cell Cycle Regulation No
Subcompartmental Group N/A

Kex1

Kex1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kex1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available