Standard name
Human Ortholog
Description Kinesin-related antiparallel sliding motor protein; involved in mitotic spindle positioning; sliding activity promotes bipolar spindle assembly and maintenance of genome stability; inhibits spindle elongation, destabilizing late anaphase spindle microtubules that polymerize beyond the midzone
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0.05
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0.05 0 0.07 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.27 0.38 0.38 0.36 0.25 0.27 0.18 – 0.1 0.13 0.11 0.09 0.36 0.38 0.45 0.17 0.18 0.11 0.17 0.19 0.19 0.25 0.2 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0.46 0.31 0.19 0.24 0.27 0.23 0.18 – 0.1 0.06 0.07 0.1 0.45 0.49 0.38 0.37 0.47 0.49 0.31 0.22 0.19 0.19 0.21 0.19
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.13 0 0.07 0 0.21 0.28 – 0.58 0.55 0.61 0.46 0 0 0 0.05 0 0 0 0.07 0.12 0.1 0.09 0.07
Nucleus 0 0 0 0 0 0.05 0 – 0.07 0.08 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.09 0 0.07 0.1 0.09 0.17 – 0.16 0.13 0.15 0.12 0.06 0.09 0.1 0.16 0.23 0.16 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.1 0.08 0.23 0.17 0.38 0.16 0.27 – 0.18 0.19 0.23 0.32 0.05 0.09 0 0.2 0.27 0.27 0.31 0.28 0.31 0.15 0.23 0.3
Vac/Vac Membrane 0.19 0.21 0.17 0.14 0.11 0.13 0.11 – 0 0.07 0.05 0.07 0.19 0.21 0.18 0.32 0.21 0.34 0 0.05 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 3 13 5 7 3 2 – 0 1 0 0 1 0 0 6 4 3 4 6 3 9 17 17
Bud 2 8 2 2 5 6 10 – 11 13 11 19 3 2 2 2 1 0 12 19 5 9 16 21
Bud Neck 0 0 5 1 3 1 2 – 8 7 3 11 0 0 0 0 0 0 3 9 4 6 4 7
Bud Site 0 0 0 1 3 4 15 – 16 13 22 9 1 0 1 0 1 0 – – – – – –
Cell Periphery 0 1 0 0 0 1 0 – 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 78 126 89 74 80 75 83 – 35 49 35 31 99 150 157 25 23 13 56 78 62 51 64 84
Endoplasmic Reticulum 6 5 8 5 2 2 5 – 1 0 2 0 1 1 2 8 1 3 0 0 3 3 11 6
Endosome 133 103 44 49 87 65 85 – 34 21 23 34 126 195 133 55 61 57 106 87 63 38 68 80
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 3 3 5 2 4 1 1 3 3
Mitochondria 15 44 4 14 11 60 129 – 203 207 194 162 11 7 9 8 5 2 13 27 41 21 27 28
Nucleus 4 2 1 6 6 15 13 – 25 28 14 18 3 1 4 1 3 1 2 7 1 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 – 0 1 2 0 0 1 0 1 0 0 2 0 1 1 1 2
Nucleolus 34 30 5 15 32 26 77 – 55 48 47 41 18 35 36 24 30 18 4 8 3 1 0 3
Peroxisomes 3 2 0 1 4 8 6 – 3 2 3 4 4 19 12 1 2 2 4 1 2 6 3 3
SpindlePole 29 26 53 35 123 44 125 – 63 70 74 112 13 35 11 30 35 31 107 111 103 31 75 125
Vac/Vac Membrane 55 69 39 29 37 36 51 – 14 25 15 24 52 84 62 47 27 39 5 21 11 9 10 14
Unique Cell Count 290 336 235 204 326 282 465 349 373 317 352 277 398 346 149 130 116 340 402 335 207 329 422
Labelled Cell Count 367 419 263 237 400 346 603 468 485 445 468 332 531 429 211 196 174 340 402 335 207 329 422


Microtubule

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 5.9 6.5 6.1 6.7 5.3 5.4 – 4.5 4.2 4.1 4.6 6.9 6.8 6.8 9.9 11.1 10.1 6.1 6.4 6.6
Std Deviation (1e-4) 1.5 1.4 1.2 1.2 1.5 1.2 1.2 – 0.9 0.9 1.2 1.0 2.5 1.8 2.0 2.2 2.5 1.9 1.5 2.0 1.8
Intensity Change (Log2) – – – -0.1 0.05 -0.29 -0.29 – -0.54 -0.62 -0.67 -0.5 0.08 0.05 0.06 0.59 0.76 0.63 -0.11 -0.03 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.6 -2.1 -2.9 -4.4 – 0 0 0 0 -3.6 0 -4.4 -0.7 -1.1 -1.2
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 2.8 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 -3.4 -2.7 -5.8 – -8.1 -7.1 -7.5 -8.6 -0.5 0 1.8 -4.4 -4.0 -5.2
Endoplasmic Reticulum -0.6 0 0 0 – 0 0 0 0 0 0 0 0.9 0 0
Endosome 1.4 2.2 1.2 -0.1 – 0 0 0 0 6.4 7.6 5.1 4.0 5.7 5.9
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.7 1.2 6.7 8.3 – 0 0 0 0 1.5 0 0.7 0 0 0
Nucleus 0 0 3.2 0 – 3.9 4.0 2.9 3.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.6 3.6 3.4 5.6 – 0 0 0 0 2.4 3.3 3.8 5.1 6.5 4.8
Peroxisomes 0 0 0 0 – 0 0 0 0 0 3.4 0 0 0 0
SpindlePole -1.4 3.8 -2.0 1.2 – 0 0 0 0 -6.0 -4.8 -7.3 -0.6 0.9 0.9
Vacuole -0.7 -1.8 -1.2 -2.1 – -5.2 -3.9 -4.6 -3.7 0.6 1.4 0.4 3.4 1.0 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0693 5.0264 4.9396 4.8579 4.7905 4.5785 2.1939 3.3394 3.3116 3.0704 2.8987 3.1683 1.8752 3.3549 3.0601 3.4048 2.9302 2.8799
Actin 0.0399 0.0138 0.0062 0.0373 0.0127 0.0069 0.0133 0.0163 0.0245 0.0062 0.022 0.0237 0.0147 0.0049 0.014 0.0295 0.022 0.0133
Bud 0.0011 0.0018 0.0007 0.0019 0.0004 0.0004 0.0011 0.0008 0.0009 0.0005 0.0014 0.0007 0.0007 0.0012 0.0004 0.0004 0.0015 0.0006
Bud Neck 0.0098 0.0086 0.0069 0.0089 0.037 0.0718 0.0024 0.0033 0.0053 0.0025 0.0048 0.0342 0.0037 0.0036 0.0053 0.0016 0.0059 0.0349
Bud Periphery 0.0016 0.0015 0.0012 0.003 0.0004 0.0007 0.001 0.0005 0.001 0.001 0.0037 0.0008 0.0007 0.0006 0.0003 0.0003 0.0013 0.0007
Bud Site 0.0244 0.0371 0.0114 0.0171 0.0027 0.0018 0.0066 0.0137 0.0203 0.0017 0.0033 0.0022 0.0037 0.0166 0.0077 0.0012 0.0032 0.0023
Cell Periphery 0.0005 0.0004 0.0002 0.0015 0.0001 0.0002 0.0002 0.0001 0.0003 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0002 0.0002
Cytoplasm 0.0814 0.0856 0.0285 0.0086 0.0075 0.0616 0.1123 0.1218 0.0451 0.0065 0.0406 0.0701 0.1611 0.1048 0.0455 0.0374 0.0278 0.0997
Cytoplasmic Foci 0.1905 0.1095 0.1379 0.1152 0.2592 0.1715 0.1657 0.1164 0.1387 0.1311 0.3043 0.1531 0.1827 0.1499 0.1051 0.0913 0.2516 0.1741
Eisosomes 0.0004 0.0002 0.0001 0.0012 0.0001 0.0001 0.0002 0 0.0002 0 0.0001 0.0001 0.0003 0 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0076 0.0045 0.0012 0.0067 0.0011 0.0044 0.009 0.0048 0.0046 0.0004 0.0039 0.0058 0.008 0.0022 0.0019 0.0007 0.0027 0.0044
Endosome 0.0822 0.0373 0.015 0.0474 0.0743 0.0604 0.0994 0.0603 0.0369 0.0165 0.1034 0.1057 0.0849 0.0362 0.0214 0.0226 0.1225 0.0615
Golgi 0.0173 0.0136 0.0053 0.0145 0.0409 0.0163 0.0344 0.011 0.0132 0.0114 0.0492 0.0337 0.0151 0.0093 0.0074 0.0124 0.0715 0.0199
Lipid Particles 0.0522 0.0356 0.0105 0.0414 0.1607 0.0771 0.0519 0.019 0.0142 0.0299 0.035 0.0677 0.0514 0.0236 0.0126 0.0462 0.0965 0.0404
Mitochondria 0.0056 0.0048 0.0026 0.0067 0.0038 0.0196 0.0139 0.0017 0.0153 0.0066 0.0151 0.0129 0.0049 0.0013 0.0071 0.001 0.0179 0.007
None 0.0533 0.0281 0.0091 0.0175 0.0015 0.019 0.0208 0.0094 0.0031 0.0082 0.0156 0.0062 0.0267 0.0091 0.0088 0.0007 0.0021 0.003
Nuclear Periphery 0.012 0.0095 0.0028 0.0077 0.0181 0.0169 0.0262 0.0187 0.0214 0.0246 0.0119 0.03 0.0227 0.0129 0.0073 0.0028 0.0056 0.0326
Nucleolus 0.0039 0.0277 0.0258 0.005 0.0049 0.0166 0.0059 0.0289 0.0079 0.0205 0.0155 0.0149 0.0024 0.0218 0.0087 0.0044 0.0029 0.0147
Nucleus 0.0287 0.0703 0.0442 0.0071 0.0043 0.0388 0.0289 0.0507 0.0317 0.0182 0.0204 0.0645 0.0249 0.1222 0.0506 0.0388 0.0045 0.0868
Peroxisomes 0.0435 0.0344 0.0259 0.0436 0.0965 0.0562 0.0406 0.036 0.016 0.0397 0.1637 0.0658 0.0444 0.0343 0.0194 0.0189 0.2372 0.0777
Punctate Nuclear 0.3343 0.4721 0.6631 0.6022 0.2721 0.3563 0.3601 0.4828 0.5956 0.6733 0.1811 0.3004 0.3434 0.4427 0.6697 0.6889 0.1187 0.3218
Vacuole 0.0075 0.003 0.0012 0.0043 0.001 0.0025 0.0047 0.0032 0.0023 0.0006 0.0029 0.0051 0.0024 0.0019 0.0055 0.0007 0.0031 0.0032
Vacuole Periphery 0.0021 0.0006 0.0002 0.0014 0.0006 0.0011 0.0015 0.0007 0.0016 0.0005 0.0018 0.0021 0.0011 0.0005 0.0012 0.0002 0.0012 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.7826 22.0283 14.227 13.1396 18.9328 14.1384 25.7786 20.6002 14.6039 20.6944
Translational Efficiency 0.5565 0.5879 0.5379 0.5201 0.604 0.702 0.5322 0.535 0.5476 0.5401

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Kinesin-related antiparallel sliding motor protein; involved in mitotic spindle positioning; sliding activity promotes bipolar spindle assembly and maintenance of genome stability; inhibits spindle elongation, destabilizing late anaphase spindle microtubules that polymerize beyond the midzone
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Kip3

Kip3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kip3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available