Standard name
Human Ortholog
Description Mitochondrial component of the ERMES complex; links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; required for mitophagy; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.06 0.06 0.07 0.12 0.08 0.06 0.11 0.17 0.17 0.13 0.17 0.16 0.05 0.06 0.05 0.08 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0.05 0.05 0.05 0.06 0 0.1 0.08 0.06 0.06 0.1 0.1 0.09 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.06 0.05 0 0.05 0 0.08 0 0 0.05 0.11 0.08 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.08 0.13 0 0.05 0.08 0 0 0 0 0 0 0 0.23 0.28 0.34 0.31 0.2 0.18 0.23 0.25 0.21 0.17 0.19 0.21
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.33 0.44 0.47 0.34 0.2 0.21 0.24 0.21 0.33 0.39 0.36 0.36 0.13 0.07 0.06 0.09 0.06 0 0 0.05 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0 0.13 0.09 0.08 0.12 0.09 0.06 0.09 0.07 0.07 0.07 0.05 0 0 0.07 0.05 0 0 0 0 0 0 0
Peroxisomes 0.35 0.45 0.14 0.28 0.35 0.16 0.16 0.1 0.09 0.13 0.12 0.14 0.54 0.56 0.48 0.43 0.44 0.36 0.26 0.32 0.34 0.35 0.35 0.41
SpindlePole 0.54 0.32 0.49 0.57 0.65 0.67 0.73 0.75 0.61 0.67 0.57 0.68 0.6 0.63 0.59 0.59 0.67 0.71 0.4 0.25 0.3 0.4 0.38 0.24
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 1 0 3 1 0 1 1 0 0 0 0 2 2 0 0 1 1 5 1 1 6 13
Bud 22 11 6 22 20 16 35 78 35 36 42 70 24 38 34 20 4 3 5 3 0 2 4 7
Bud Neck 4 2 0 0 4 2 6 13 4 5 4 3 0 2 4 3 1 1 3 2 0 1 0 6
Bud Site 20 10 4 11 5 24 27 28 13 26 25 39 12 5 12 8 3 4 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 3 2 5 7 14 15 19 10 12 19 10 11 32 73 21 4 0 0 1 0 0 1 1
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 1 0 0 0 0 0 3 2 0 2 1 0 0 0 1 2
Endosome 30 25 2 9 19 6 3 6 1 3 5 5 99 172 228 79 25 16 87 51 25 53 82 111
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 10 8 1 5 4 12
Mitochondria 120 85 40 60 47 54 81 92 68 103 87 156 55 45 41 22 7 3 9 9 6 8 9 20
Nucleus 3 0 1 1 1 4 3 4 7 5 2 3 2 0 1 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 1 1 0 1 0 0 0 2 0 0 4 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 41 8 11 16 20 29 29 25 19 20 16 29 22 20 17 17 6 4 2 0 0 1 0 1
Peroxisomes 129 86 12 49 84 41 52 45 18 36 29 60 235 342 323 110 56 33 102 63 39 109 152 220
SpindlePole 197 61 42 100 155 168 242 337 127 180 138 295 264 381 403 153 85 65 156 50 34 126 165 125
Vac/Vac Membrane 2 3 0 2 1 0 0 0 0 0 0 0 10 6 8 8 3 4 1 2 0 0 0 1
Unique Cell Count 366 192 85 176 239 252 333 447 208 267 242 437 437 608 679 258 126 91 390 202 117 316 437 534
Labelled Cell Count 575 299 122 275 367 359 493 648 306 426 367 674 734 1046 1149 445 197 136 390 202 117 316 437 534


Mitochondria, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.3 4.5 4.6 4.6 3.9 3.2 3.6 3.5 2.9 3.3 2.8 6.0 5.4 4.7 6.8 7.1 7.7 5.5 5.1 5.8
Std Deviation (1e-4) 0.7 0.9 0.8 1.5 1.3 1.4 1.1 1.0 1.6 1.1 1.7 1.2 1.6 1.5 1.5 2.2 2.0 2.2 1.3 1.2 1.8
Intensity Change (Log2) – – – 0.04 0.02 -0.22 -0.48 -0.34 -0.38 -0.62 -0.47 -0.68 0.4 0.25 0.06 0.59 0.65 0.77 0.27 0.17 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – 0 – 0 -0.3 0 0.2 0 0
Bud Neck – – – – – – – 0 – 0 0 0 0 0 0
Bud Site – – – – – – – 0 – 0 0 0 0 0 0
Cell Periphery – – – – – – – 0 – 0 0 0 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – 0 – 0 0 0 0 0 0
Endosome – – – – – – – 0 – 4.3 5.2 0 5.3 3.7 3.3
Golgi – – – – – – – 0 – 0 0 0 0 0 0
Mitochondria – – – – – – – 0 – -7.5 -10.4 0 -8.0 -7.1 -6.8
Nuclear Periphery – – – – – – – 0 – 0 0 0 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – 0 – -2.7 0 0 -1.9 -2.1 -2.0
Peroxisomes – – – – – – – 0 – 6.7 7.3 0 4.8 4.6 3.4
SpindlePole – – – – – – – 0 – 1.9 2.3 0 1.6 2.6 3.0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – 0 – 0 0 0 0 0 0
Cytoplasm – – – – – – – 1.8 – 0 0 2.5 1.8 0 0
Nucleus – – – – – – – 0 – 0 0 0 0 0 0
Vacuole – – – – – – – 0 – 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2601 3.3116 3.0986 2.4052 2.7848 2.9926 1.6946 1.9461 1.7901 1.0873 1.6142 1.86 3.9296 4.2401 3.6315 4.1946 4.0041 3.9836
Actin 0.0245 0.013 0.0144 0.0013 0.0035 0.0151 0.0388 0.0073 0.0112 0.0061 0.006 0.0081 0.0204 0.0053 0.0438 0.0086 0.0045 0.0134
Bud 0.0005 0.0015 0.001 0.0001 0.0002 0.0004 0.0005 0.0003 0.0002 0.0002 0.0001 0.0003 0.0003 0.0008 0.0004 0.0001 0.0001 0.0002
Bud Neck 0.0028 0.0007 0.0011 0.0002 0.0005 0.0096 0.0012 0.0009 0.0009 0.0006 0.0003 0.0006 0.0005 0.0004 0.0005 0.0106 0.0004 0.0044
Bud Periphery 0.0004 0.001 0.0011 0 0.0002 0.0004 0.0006 0.0002 0.0002 0.0002 0.0001 0.0006 0.0003 0.0006 0.0003 0.0001 0 0.0002
Bud Site 0.0032 0.0161 0.0133 0.0002 0.0004 0.0016 0.0048 0.002 0.0007 0.0024 0.0003 0.0017 0.0019 0.0117 0.0023 0.0005 0.0002 0.0005
Cell Periphery 0.0001 0.0001 0.0005 0 0 0.0001 0.0001 0 0.0001 0.0001 0 0.0001 0.0001 0 0.0002 0 0 0
Cytoplasm 0.0027 0.0071 0.003 0.0007 0.0012 0.0027 0.0047 0.0013 0.0015 0.0003 0.0006 0.0005 0.0138 0.0007 0.0073 0.0007 0.0003 0.0013
Cytoplasmic Foci 0.249 0.2791 0.2583 0.2275 0.2259 0.2279 0.2937 0.2923 0.2832 0.2305 0.3233 0.254 0.3038 0.3784 0.3015 0.2567 0.1561 0.2507
Eisosomes 0.0002 0 0.0002 0 0 0 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0024 0.0008 0.0007 0 0.0002 0.0002 0.0012 0.0002 0.0028 0.0001 0.0002 0.0004 0.0027 0 0.0023 0 0.0001 0.0001
Endosome 0.0784 0.0489 0.0533 0.0324 0.0941 0.0444 0.0585 0.0513 0.0499 0.0375 0.062 0.0191 0.0572 0.0238 0.0277 0.025 0.0437 0.033
Golgi 0.0476 0.0332 0.0501 0.033 0.1199 0.0478 0.0397 0.028 0.0509 0.069 0.0534 0.0366 0.0577 0.0179 0.0416 0.0354 0.0343 0.0491
Lipid Particles 0.1213 0.0634 0.0699 0.1059 0.0615 0.1282 0.1501 0.0525 0.0838 0.0776 0.0436 0.12 0.0961 0.0704 0.0596 0.0861 0.0248 0.0982
Mitochondria 0.0099 0.0137 0.0245 0.0014 0.0627 0.0156 0.0172 0.0022 0.0341 0.0302 0.0647 0.0072 0.0202 0.0036 0.0191 0.002 0.0041 0.0109
None 0.0042 0.0006 0.0044 0 0.0001 0.0002 0.0058 0.0002 0.0024 0.0001 0.0003 0.0005 0.0031 0.0004 0.0006 0.0001 0.0001 0.0002
Nuclear Periphery 0.005 0.0009 0.004 0 0.0002 0.0021 0.0119 0.0002 0.011 0 0.0003 0.0004 0.0081 0 0.004 0 0 0.0001
Nucleolus 0.0015 0.0008 0.0012 0 0.0001 0.0006 0.0008 0.001 0.0002 0.0006 0.0001 0.0004 0.0006 0.0006 0.0002 0 0 0.0001
Nucleus 0.0019 0.0013 0.0011 0 0.0001 0.0018 0.0066 0.0011 0.0011 0.0001 0.0001 0.0004 0.0039 0.0001 0.0004 0 0 0.0001
Peroxisomes 0.416 0.5011 0.4807 0.5958 0.3942 0.4797 0.3482 0.534 0.4583 0.4675 0.4375 0.5402 0.3713 0.4809 0.459 0.5724 0.7297 0.5283
Punctate Nuclear 0.0247 0.007 0.0104 0.0013 0.0337 0.0189 0.0134 0.0239 0.006 0.0751 0.0056 0.0082 0.0362 0.0038 0.0277 0.0016 0.0007 0.0085
Vacuole 0.0028 0.0075 0.005 0.0001 0.0007 0.0018 0.001 0.0009 0.0008 0.0011 0.0007 0.0005 0.0011 0.0005 0.001 0.0001 0.0005 0.0004
Vacuole Periphery 0.0009 0.0022 0.0018 0.0001 0.0006 0.0009 0.0009 0.0002 0.0008 0.0004 0.0005 0.0003 0.0008 0.0001 0.0005 0.0001 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.5883 14.2953 6.895 20.4727 13.4768 20.7773 22.223 20.2426 22.406 22.5723
Translational Efficiency 0.5859 0.8659 1.9788 0.7196 1.003 0.8886 0.6963 0.8901 0.7466 0.7551

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial component of the ERMES complex; links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; required for mitophagy; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase
Localization
Cell Percentages spindle pole (38%), peroxisome (10%), mixed (25%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mdm34

Mdm34


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mdm34-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available