Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 13 | 27 | 48 | 16 | 11 | 5 | 4 | 15 | 4 | 3 | 10 | 6 | 2 | 7 | 3 | 14 | 18 | 15 | 15 | 18 | 35 | 2 | 9 | 15 |
Bud | 5 | 20 | 14 | 8 | 12 | 9 | 16 | 18 | 19 | 13 | 10 | 21 | 0 | 5 | 1 | 4 | 5 | 4 | 7 | 6 | 8 | 6 | 6 | 5 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 7 | 5 | 2 | 2 | 3 |
Bud Site | 0 | 2 | 1 | 0 | 1 | 0 | 2 | 3 | 2 | 0 | 4 | 5 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 2 | 12 | 14 | 13 | 3 | 2 | 7 | 5 | 3 | 2 | 7 | 11 | 0 | 3 | 0 | 15 | 20 | 19 | 0 | 0 | 3 | 0 | 0 | 0 |
Cytoplasm | 76 | 104 | 85 | 85 | 74 | 91 | 65 | 68 | 46 | 33 | 39 | 50 | 18 | 35 | 14 | 204 | 137 | 189 | 59 | 45 | 22 | 13 | 12 | 10 |
Endoplasmic Reticulum | 13 | 19 | 7 | 9 | 7 | 12 | 9 | 7 | 9 | 10 | 10 | 22 | 5 | 2 | 9 | 15 | 10 | 11 | 6 | 4 | 7 | 1 | 1 | 3 |
Endosome | 27 | 53 | 97 | 60 | 46 | 99 | 117 | 85 | 22 | 48 | 53 | 59 | 5 | 33 | 30 | 80 | 56 | 64 | 69 | 99 | 107 | 21 | 33 | 26 |
Golgi | 4 | 9 | 22 | 14 | 7 | 13 | 11 | 10 | 2 | 11 | 8 | 11 | 0 | 12 | 24 | 10 | 23 | 11 | 10 | 28 | 40 | 14 | 22 | 29 |
Mitochondria | 25 | 118 | 18 | 72 | 73 | 140 | 215 | 198 | 371 | 308 | 423 | 444 | 6 | 19 | 45 | 28 | 17 | 7 | 48 | 176 | 229 | 78 | 80 | 104 |
Nucleus | 1 | 1 | 1 | 2 | 4 | 2 | 5 | 18 | 16 | 7 | 8 | 5 | 1 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 1 | 9 | 24 | 15 |
Nuclear Periphery | 1 | 2 | 0 | 0 | 1 | 0 | 1 | 3 | 3 | 7 | 3 | 7 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 1 | 2 |
Nucleolus | 1 | 1 | 0 | 4 | 3 | 1 | 7 | 9 | 9 | 7 | 8 | 10 | 0 | 0 | 1 | 4 | 2 | 3 | 1 | 0 | 0 | 9 | 17 | 12 |
Peroxisomes | 0 | 3 | 4 | 1 | 0 | 0 | 6 | 0 | 1 | 1 | 3 | 4 | 0 | 1 | 6 | 7 | 1 | 4 | 0 | 6 | 10 | 3 | 5 | 5 |
SpindlePole | 2 | 14 | 73 | 21 | 10 | 16 | 21 | 24 | 8 | 18 | 14 | 21 | 0 | 4 | 6 | 38 | 44 | 42 | 9 | 16 | 39 | 6 | 11 | 6 |
Vac/Vac Membrane | 42 | 69 | 49 | 51 | 53 | 154 | 162 | 172 | 92 | 85 | 90 | 103 | 7 | 13 | 17 | 52 | 72 | 111 | 14 | 40 | 11 | 18 | 24 | 22 |
Unique Cell Count | 177 | 359 | 360 | 282 | 258 | 455 | 526 | 539 | 504 | 456 | 557 | 633 | 39 | 115 | 131 | 379 | 305 | 384 | 260 | 465 | 547 | 194 | 258 | 271 |
Labelled Cell Count | 212 | 454 | 434 | 356 | 306 | 544 | 648 | 636 | 607 | 553 | 690 | 779 | 44 | 134 | 157 | 471 | 407 | 482 | 260 | 465 | 547 | 194 | 258 | 271 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.7 | 5.9 | 8.1 | 7.8 | 6.7 | 7.2 | 6.9 | 6.9 | 6.1 | 6.4 | 6.1 | 6.2 | 7.5 | 7.9 | 7.2 | 8.0 | 10.3 | 10.7 | 9.0 | 9.4 | 9.3 |
Std Deviation (1e-4) | 1.5 | 1.0 | 1.2 | 1.3 | 1.5 | 1.2 | 1.4 | 1.2 | 1.3 | 1.0 | 1.1 | 1.1 | 1.2 | 1.4 | 1.3 | 1.6 | 1.9 | 2.1 | 2.5 | 3.0 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.27 | -0.16 | -0.22 | -0.22 | -0.41 | -0.34 | -0.41 | -0.39 | -0.11 | -0.03 | -0.16 | -0.02 | 0.35 | 0.41 | 0.17 | 0.22 | 0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -3.2 | -3.8 | -7.0 | -7.8 | -6.1 | -7.6 | -7.4 | -7.0 | -8.3 | 0 | -2.1 | -3.5 | -4.7 | -3.2 | -4.6 |
Bud | -0.7 | 0.5 | -1.6 | -0.7 | -0.4 | -0.1 | -0.8 | -1.9 | -0.5 | 0 | 0 | 0 | -2.5 | -1.7 | -2.5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.5 | -2.0 | -3.5 | -2.5 | -3.0 | -3.4 | -3.5 | -2.6 | -2.1 | 0 | 0 | 0 | 0 | 1.6 | 0.7 |
Cytoplasm | 1.9 | 1.4 | -1.2 | -4.4 | -4.3 | -5.9 | -6.6 | -7.2 | -6.9 | 3.1 | 1.5 | -3.2 | 8.4 | 5.8 | 7.2 |
Endoplasmic Reticulum | 1.0 | 0.6 | 0.7 | -0.3 | -0.8 | -0.2 | 0.2 | -0.2 | 1.4 | 0 | 0 | 0 | 1.6 | 1.1 | 0.8 |
Endosome | -1.7 | -2.6 | -1.7 | -1.6 | -4.1 | -9.5 | -6.1 | -7.0 | -7.3 | -1.9 | 0.4 | -0.9 | -1.9 | -2.6 | -3.4 |
Golgi | -0.6 | -2.0 | -2.3 | -3.1 | -3.4 | -5.0 | -2.7 | -3.9 | -3.7 | 0 | 1.6 | 4.1 | -2.3 | 0.7 | -2.1 |
Mitochondria | 7.4 | 8.1 | 9.2 | 11.9 | 10.9 | 20.0 | 18.1 | 21.0 | 19.8 | 0 | 4.0 | 8.6 | 1.3 | 0.3 | -2.4 |
Nucleus | 0 | 0 | 0 | 0 | 3.1 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 |
SpindlePole | -4.6 | -5.9 | -7.6 | -7.7 | -7.5 | -9.3 | -7.4 | -9.0 | -8.8 | -3.1 | -4.3 | -4.2 | -3.9 | -2.0 | -3.5 |
Vacuole | 1.6 | 2.3 | 6.6 | 5.9 | 6.2 | 1.8 | 1.9 | 1.1 | 1.1 | 0.7 | -0.6 | -0.2 | 0 | 3.3 | 5.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.0601 | 7.8416 | 7.1517 | 6.7803 | 6.5161 | 7.1758 | 9.7681 | 11.2126 | 11.4592 | 10.3846 | 10.3395 | 10.5363 | 8.6469 | 9.5168 | 9.0064 | 8.9995 | 8.5121 | 9.0174 |
Actin | 0.0513 | 0.0225 | 0.0557 | 0.0212 | 0.1199 | 0.0214 | 0.054 | 0.0279 | 0.1319 | 0.0749 | 0.0373 | 0.0578 | 0.014 | 0.0098 | 0.0208 | 0.0153 | 0.0268 | 0.0177 |
Bud | 0.0036 | 0.0046 | 0.0118 | 0.0064 | 0.006 | 0.0035 | 0.002 | 0.0081 | 0.0141 | 0.0089 | 0.0086 | 0.0085 | 0.0037 | 0.0057 | 0.0055 | 0.0182 | 0.0072 | 0.0029 |
Bud Neck | 0.0037 | 0.0025 | 0.0044 | 0.0165 | 0.0022 | 0.0042 | 0.0034 | 0.0017 | 0.0027 | 0.0024 | 0.0011 | 0.0061 | 0.0034 | 0.0018 | 0.0023 | 0.0024 | 0.0037 | 0.0058 |
Bud Periphery | 0.0053 | 0.0039 | 0.0136 | 0.0085 | 0.0041 | 0.0047 | 0.0019 | 0.0049 | 0.0231 | 0.0114 | 0.0075 | 0.0063 | 0.0053 | 0.0037 | 0.0056 | 0.02 | 0.0119 | 0.0033 |
Bud Site | 0.0142 | 0.026 | 0.0242 | 0.0084 | 0.0051 | 0.0028 | 0.0175 | 0.0302 | 0.0468 | 0.0145 | 0.0139 | 0.0049 | 0.0084 | 0.0224 | 0.0084 | 0.0062 | 0.0087 | 0.0026 |
Cell Periphery | 0.0038 | 0.0014 | 0.0026 | 0.0008 | 0.0004 | 0.0009 | 0.0003 | 0.0007 | 0.0026 | 0.0016 | 0.0003 | 0.0007 | 0.0012 | 0.0006 | 0.0012 | 0.001 | 0.0007 | 0.0005 |
Cytoplasm | 0.1487 | 0.3168 | 0.2413 | 0.2456 | 0.233 | 0.2332 | 0.0845 | 0.324 | 0.2845 | 0.2226 | 0.1186 | 0.2147 | 0.1888 | 0.3646 | 0.3044 | 0.35 | 0.2182 | 0.2825 |
Cytoplasmic Foci | 0.106 | 0.1695 | 0.1076 | 0.1622 | 0.1365 | 0.1197 | 0.1717 | 0.1587 | 0.1279 | 0.0369 | 0.1089 | 0.1386 | 0.1315 | 0.1409 | 0.1266 | 0.1374 | 0.1244 | 0.1342 |
Eisosomes | 0.0005 | 0.0002 | 0.0006 | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0005 | 0.001 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0219 | 0.009 | 0.0109 | 0.006 | 0.0078 | 0.0149 | 0.0071 | 0.0072 | 0.0168 | 0.0078 | 0.0047 | 0.0095 | 0.0235 | 0.0087 | 0.0115 | 0.0093 | 0.0114 | 0.0177 |
Endosome | 0.2586 | 0.1646 | 0.1581 | 0.1585 | 0.2427 | 0.1677 | 0.145 | 0.1609 | 0.114 | 0.099 | 0.2175 | 0.1937 | 0.2878 | 0.2106 | 0.184 | 0.1703 | 0.2478 | 0.2407 |
Golgi | 0.1323 | 0.1386 | 0.122 | 0.1253 | 0.1444 | 0.1465 | 0.1319 | 0.1366 | 0.1413 | 0.223 | 0.1448 | 0.1776 | 0.12 | 0.1132 | 0.1106 | 0.0675 | 0.1743 | 0.1311 |
Lipid Particles | 0.0161 | 0.0038 | 0.0054 | 0.0127 | 0.0069 | 0.0044 | 0.0166 | 0.0111 | 0.0046 | 0.0011 | 0.001 | 0.0031 | 0.006 | 0.0026 | 0.0023 | 0.0009 | 0.0027 | 0.0017 |
Mitochondria | 0.1445 | 0.0895 | 0.2067 | 0.1873 | 0.0365 | 0.2399 | 0.0621 | 0.087 | 0.0462 | 0.275 | 0.2459 | 0.1361 | 0.1411 | 0.0845 | 0.1799 | 0.1689 | 0.0993 | 0.1146 |
None | 0.0158 | 0.0027 | 0.0051 | 0.002 | 0.0213 | 0.0014 | 0.03 | 0.0015 | 0.0044 | 0.0016 | 0.0008 | 0.0012 | 0.0042 | 0.002 | 0.0025 | 0.0017 | 0.0019 | 0.0016 |
Nuclear Periphery | 0.0127 | 0.0014 | 0.003 | 0.0023 | 0.0022 | 0.0013 | 0.0128 | 0.0013 | 0.0012 | 0.0012 | 0.0005 | 0.0023 | 0.0115 | 0.0026 | 0.0041 | 0.0007 | 0.0048 | 0.0058 |
Nucleolus | 0.0006 | 0.0006 | 0.0007 | 0.0006 | 0.0002 | 0.0002 | 0.0174 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0009 | 0.0001 |
Nucleus | 0.0088 | 0.0019 | 0.002 | 0.0017 | 0.0014 | 0.0014 | 0.1069 | 0.0011 | 0.0009 | 0.0009 | 0.0005 | 0.0016 | 0.0037 | 0.0019 | 0.0032 | 0.0012 | 0.0024 | 0.0031 |
Peroxisomes | 0.0216 | 0.0263 | 0.0108 | 0.0212 | 0.0157 | 0.0204 | 0.0385 | 0.0242 | 0.0306 | 0.0116 | 0.0826 | 0.0232 | 0.0222 | 0.0105 | 0.0143 | 0.0146 | 0.0187 | 0.0191 |
Punctate Nuclear | 0.0036 | 0.0012 | 0.0016 | 0.0046 | 0.0023 | 0.0007 | 0.0844 | 0.0035 | 0.0009 | 0.0002 | 0.0003 | 0.002 | 0.0028 | 0.0007 | 0.0017 | 0.0005 | 0.0006 | 0.0007 |
Vacuole | 0.0182 | 0.0102 | 0.0079 | 0.0051 | 0.0076 | 0.0061 | 0.0085 | 0.0058 | 0.0032 | 0.0017 | 0.0022 | 0.007 | 0.013 | 0.0093 | 0.0072 | 0.0085 | 0.0261 | 0.0083 |
Vacuole Periphery | 0.0082 | 0.0029 | 0.0039 | 0.0027 | 0.0035 | 0.0043 | 0.0034 | 0.0035 | 0.0017 | 0.0027 | 0.0026 | 0.0044 | 0.007 | 0.0036 | 0.0034 | 0.005 | 0.0073 | 0.0059 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 63.6335 | 53.4714 | 56.5623 | 61.1709 | 62.7027 | 55.9665 | 61.5419 | 75.6976 | 66.2612 | 68.8794 |
Translational Efficiency | 1.6072 | 1.684 | 1.4266 | 1.4384 | 1.3756 | 1.6263 | 1.6775 | 1.2419 | 1.4777 | 1.4589 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
207 | 1099 | 2875 | 1531 | 1863 | 2516 | 2714 | 1896 | 2070 | 3615 | 5589 | 3427 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 723.67 | 970.73 | 1221.52 | 1218.20 | 852.83 | 1067.83 | 1167.19 | 1282.56 | 839.91 | 1038.31 | 1195.14 | 1253.81 |
Standard Deviation | 99.11 | 153.38 | 172.71 | 197.56 | 117.40 | 142.82 | 168.92 | 202.88 | 122.02 | 152.78 | 173.02 | 203.06 |
Intensity Change Log 2 | — | 0.423738 | 0.755274 | 0.751347 | — | 0.324352 | 0.452709 | 0.588696 | — | 0.370825 | 0.599507 | 0.665641 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000539 | 0.004039 | 0.003843 | 0.027950 | 0.002149 | 0.003072 | 0.005391 | 0.017518 | 0.001988 | 0.003366 | 0.004595 | 0.022178 |
Bud Neck | 0.012445 | 0.008036 | 0.024493 | 0.005149 | 0.008669 | 0.010126 | 0.014362 | 0.004419 | 0.009047 | 0.009491 | 0.019573 | 0.004745 |
Bud Site | 0.012982 | 0.108640* | 0.065796 | 0.161023* | 0.040081 | 0.053122 | 0.123882* | 0.167732* | 0.037371 | 0.070000 | 0.094002 | 0.164735* |
Cell Periphery | 0.000472 | 0.000251 | 0.000091 | 0.000430 | 0.000308 | 0.000177 | 0.000163 | 0.000392 | 0.000325 | 0.000199 | 0.000126 | 0.000409 |
Cytoplasm | 0.003652 | 0.074979 | 0.493315* | 0.072249 | 0.055766 | 0.048336 | 0.130847* | 0.113013* | 0.050554 | 0.056436 | 0.317302* | 0.094802 |
Cytoplasmic Foci | 0.056434 | 0.198586* | 0.059144 | 0.096192 | 0.260791* | 0.169294 | 0.103078 | 0.107778 | 0.240355* | 0.178199 | 0.080478 | 0.102602 |
Eisosomes | 0.000080 | 0.000070 | 0.000024 | 0.000097 | 0.000120 | 0.000080 | 0.000039 | 0.000075 | 0.000116 | 0.000077 | 0.000032 | 0.000084 |
Endoplasmic Reticulum | 0.001415 | 0.001320 | 0.001559 | 0.001179 | 0.003251 | 0.000638 | 0.000953 | 0.000777 | 0.003068 | 0.000846 | 0.001265 | 0.000956 |
Endosome | 0.086526 | 0.114202 | 0.013263 | 0.091445 | 0.112997 | 0.128526 | 0.096339 | 0.074993 | 0.110350 | 0.124171 | 0.053604 | 0.082343 |
Golgi | 0.097275 | 0.181161* | 0.013165 | 0.181297* | 0.174781* | 0.205293* | 0.076473 | 0.187137* | 0.167030* | 0.197956* | 0.043907 | 0.184528* |
Lipid Particles | 0.004853 | 0.001484 | 0.001443 | 0.001113 | 0.006712 | 0.001056 | 0.000888 | 0.000676 | 0.006526 | 0.001186 | 0.001174 | 0.000871 |
Mitochondria | 0.615563* | 0.134926* | 0.015399 | 0.128757* | 0.209294* | 0.247798* | 0.086164 | 0.115557* | 0.249921* | 0.213484* | 0.049763 | 0.121454* |
Mitotic Spindle | 0.008135 | 0.056073 | 0.112597* | 0.111071* | 0.022923 | 0.038011 | 0.132357* | 0.100099* | 0.021444 | 0.043502 | 0.122193* | 0.105001* |
None | 0.000561 | 0.000454 | 0.002454 | 0.000282 | 0.000366 | 0.000387 | 0.000358 | 0.000343 | 0.000386 | 0.000407 | 0.001436 | 0.000316 |
Nuclear Periphery | 0.000223 | 0.000099 | 0.000927 | 0.000244 | 0.000249 | 0.000090 | 0.000392 | 0.000099 | 0.000247 | 0.000093 | 0.000667 | 0.000164 |
Nuclear Periphery Foci | 0.001034 | 0.000874 | 0.001301 | 0.001015 | 0.000833 | 0.000226 | 0.001110 | 0.000489 | 0.000853 | 0.000423 | 0.001208 | 0.000724 |
Nucleolus | 0.004604 | 0.000438 | 0.001047 | 0.000171 | 0.000464 | 0.000276 | 0.000756 | 0.000094 | 0.000878 | 0.000325 | 0.000906 | 0.000128 |
Nucleus | 0.001480 | 0.001798 | 0.102944 | 0.000714 | 0.002690 | 0.001131 | 0.003537 | 0.000744 | 0.002569 | 0.001334 | 0.054672 | 0.000731 |
Peroxisomes | 0.048552 | 0.036217 | 0.010456 | 0.035232 | 0.019190 | 0.031230 | 0.020560 | 0.026064 | 0.022126 | 0.032746 | 0.015362 | 0.030160 |
Vacuole | 0.016960 | 0.050463 | 0.072706 | 0.060535 | 0.049802 | 0.041491 | 0.166870 | 0.059268 | 0.046518 | 0.044219 | 0.118431 | 0.059834 |
Vacuole Periphery | 0.026215 | 0.025892 | 0.004033 | 0.023855 | 0.028562 | 0.019639 | 0.035482 | 0.022734 | 0.028327 | 0.021540 | 0.019304 | 0.023235 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.52 | -5.80 | -11.54 | -9.75 | -10.24 | -1.34 | -4.70 | -9.77 | -8.76 | -7.28 | -2.55 | -4.95 | -14.50 | -13.11 | -12.64 |
Bud Neck | 0.99 | -2.60 | 1.53 | 2.80 | 16.44 | -1.49 | -7.01 | 5.05 | 6.85 | 14.21 | -0.49 | -10.96 | 4.78 | 7.20 | 21.54 |
Bud Site | -14.74 | -8.76 | -22.43 | -7.60 | -19.41 | -4.45 | -21.66 | -25.30 | -22.42 | -8.41 | -11.42 | -18.38 | -32.64 | -23.44 | -20.00 |
Cell Periphery | 1.87 | 3.20 | 0.40 | -5.84 | -15.58 | 1.80 | 2.13 | -1.06 | -8.30 | -10.27 | 1.89 | 3.05 | -1.19 | -10.61 | -18.16 |
Cytoplasm | -15.69 | -88.12* | -21.25 | -0.22 | 63.95* | 1.97 | -18.73 | -12.79 | -15.01 | 4.75 | -1.97 | -56.27 | -13.41 | -12.57 | 44.64* |
Cytoplasmic Foci | -16.80 | 0.91 | -5.31 | 17.78 | -13.78 | 16.72 | 32.18 | 29.39 | 15.23 | -2.21 | 12.26 | 36.65 | 29.40 | 23.21 | -11.30 |
Eisosomes | 0.99 | 5.75 | -1.48 | -5.22 | -18.56 | 6.46 | 15.71 | 8.24 | 1.51 | -11.74 | 7.30 | 18.07 | 6.43 | -1.75 | -21.96 |
Endoplasmic Reticulum | 0.08 | -0.51 | 0.22 | 0.39 | 2.36 | 5.34 | 4.46 | 4.97 | -1.49 | 1.37 | 4.90 | 3.54 | 4.53 | -0.96 | 3.07 |
Endosome | -2.48 | 7.45 | -0.20 | 5.25 | -26.79* | -3.44 | 6.05 | 10.45 | 14.95 | 5.26 | -3.44 | 18.50 | 8.59 | 14.73 | -14.72 |
Golgi | -6.82 | 8.41 | -6.37 | 0.80 | -29.28* | -4.86 | 20.49 | -0.67 | 3.98 | -20.20* | -5.51 | 28.71 | -1.69 | 4.12 | -35.46* |
Lipid Particles | 2.40 | 2.09 | 2.54 | 0.39 | 3.18 | 7.71 | 7.68 | 8.07 | 0.74 | 1.96 | 7.92 | 7.47 | 8.18 | 0.45 | 4.15 |
Mitochondria | 18.77* | 24.31 | 19.34 | 1.27 | -19.33* | -4.60 | 19.21 | 12.87 | 18.81 | -6.11 | 4.59 | 30.26 | 17.74 | 16.83 | -18.54 |
Mitotic Spindle | -11.48 | -22.86* | -19.16 | -8.68 | -1.51 | -5.59 | -24.07* | -17.61 | -13.89 | 5.16 | -9.09 | -29.52 | -23.53 | -16.85 | 2.42 |
None | 0.66 | -12.49 | 1.98 | 1.84 | 24.77* | -0.16 | -0.19 | -0.20 | 0.02 | -0.12 | -0.22 | -19.37 | 0.73 | 0.59 | 16.36 |
Nuclear Periphery | 1.05 | -8.47 | -0.30 | -1.92 | 9.73 | 3.31 | -3.59 | 2.81 | -1.50 | 8.40 | 3.43 | -10.83 | 1.16 | -2.31 | 12.75 |
Nuclear Periphery Foci | 0.20 | -1.19 | -0.12 | -0.43 | 1.80 | 3.42 | -1.74 | 0.95 | -3.06 | 2.64 | 2.11 | -3.28 | 0.01 | -2.13 | 3.35 |
Nucleolus | 1.51 | 1.19 | 1.61 | 2.16 | 8.19 | 1.63 | -3.79 | 5.00 | 2.16 | 8.96 | 1.87 | -0.82 | 2.57 | 2.68 | 11.86 |
Nucleus | -0.73 | -40.81 | 0.04 | 1.09 | 41.42 | 3.96 | -6.28 | 3.24 | -0.71 | 8.44 | 3.40 | -36.92 | 3.45 | -0.08 | 37.63 |
Peroxisomes | 1.40 | 4.76 | 1.57 | 0.39 | -12.15 | -5.93 | -0.15 | -3.43 | 2.59 | -3.41 | -5.46 | 5.82 | -4.13 | 1.63 | -11.67 |
Vacuole | -7.57 | -17.72 | -10.33 | -3.88 | 6.84 | 2.97 | -28.69 | -4.42 | -7.63 | 24.40 | 0.81 | -28.70 | -6.77 | -8.73 | 22.57 |
Vacuole Periphery | 0.13 | 5.89 | 0.76 | 1.16 | -12.46 | 5.75 | -1.84 | 3.63 | -1.52 | 5.36 | 4.68 | 7.96 | 3.71 | -0.75 | -4.40 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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