Standard name | |
---|---|
Human Ortholog | |
Description | Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 32 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 15 | 8 | 8 | 7 | 3 | 2 | 3 | 11 | 5 | 8 | 19 | 16 |
Bud | 4 | 6 | 7 | 5 | 3 | 5 | 23 | 8 | 8 | 13 | 9 | 7 | 1 | 1 | 4 | 5 | 7 | 8 | 0 | 1 | 1 | 1 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 1 | 5 | 6 | 2 | 6 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 4 | 0 | 2 | 0 | 3 | 0 | 3 | 3 | 4 | 1 | 1 | 1 | 1 | 5 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 25 | 1 | 5 | 7 | 9 | 4 | 5 | 4 | 5 | 3 | 5 | 25 | 32 | 47 | 40 | 29 | 22 | 0 | 1 | 0 | 3 | 5 | 2 |
Endoplasmic Reticulum | 10 | 47 | 5 | 2 | 1 | 0 | 0 | 0 | 4 | 2 | 2 | 0 | 16 | 11 | 12 | 9 | 11 | 9 | 0 | 2 | 0 | 1 | 6 | 6 |
Endosome | 2 | 3 | 20 | 14 | 7 | 1 | 0 | 4 | 4 | 2 | 1 | 0 | 42 | 90 | 108 | 60 | 63 | 50 | 26 | 49 | 22 | 35 | 42 | 25 |
Golgi | 20 | 92 | 61 | 22 | 12 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 171 | 299 | 283 | 168 | 166 | 84 | 166 | 296 | 196 | 317 | 443 | 422 |
Mitochondria | 222 | 350 | 205 | 139 | 132 | 132 | 261 | 234 | 198 | 264 | 166 | 205 | 90 | 79 | 87 | 44 | 34 | 23 | 21 | 71 | 20 | 62 | 46 | 21 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 3 | 2 | 3 | 1 | 0 | 1 | 0 | 1 | 2 | 0 |
Nuclear Periphery | 1 | 2 | 1 | 2 | 0 | 2 | 0 | 2 | 3 | 2 | 0 | 0 | 1 | 2 | 3 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 3 |
Nucleolus | 2 | 1 | 1 | 3 | 0 | 2 | 5 | 11 | 3 | 3 | 4 | 2 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 8 | 8 | 1 | 4 | 7 | 6 | 4 | 8 | 7 | 13 | 1 | 1 | 1 | 6 | 7 | 15 | 7 | 2 | 14 | 4 | 9 | 15 |
SpindlePole | 0 | 1 | 4 | 1 | 1 | 1 | 5 | 4 | 9 | 4 | 2 | 2 | 7 | 23 | 25 | 21 | 14 | 15 | 2 | 1 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 9 | 22 | 6 | 0 | 5 | 2 | 4 | 4 | 9 | 0 | 0 | 4 | 36 | 86 | 57 | 68 | 39 | 50 | 1 | 19 | 1 | 14 | 9 | 4 |
Unique Cell Count | 231 | 407 | 242 | 156 | 149 | 139 | 273 | 247 | 210 | 275 | 174 | 212 | 308 | 516 | 523 | 358 | 315 | 232 | 234 | 461 | 264 | 456 | 591 | 525 |
Labelled Cell Count | 281 | 583 | 325 | 203 | 171 | 159 | 318 | 288 | 254 | 317 | 201 | 240 | 407 | 633 | 639 | 441 | 381 | 287 | 234 | 461 | 264 | 456 | 591 | 525 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 7.1 | 7.0 | 6.4 | 6.2 | 5.6 | 5.3 | 5.1 | 5.1 | 4.7 | 4.4 | 4.7 | 8.7 | 8.4 | 8.3 | 9.2 | 8.5 | 8.1 | 9.7 | 9.8 | 10.2 |
Std Deviation (1e-4) | 0.8 | 1.0 | 0.9 | 0.9 | 1.4 | 2.1 | 1.4 | 1.1 | 1.3 | 1.0 | 1.1 | 0.8 | 1.0 | 1.0 | 1.3 | 1.6 | 1.6 | 1.8 | 1.3 | 1.3 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.16 | -0.31 | -0.39 | -0.46 | -0.46 | -0.57 | -0.66 | -0.57 | 0.32 | 0.27 | 0.25 | 0.4 | 0.29 | 0.22 | 0.47 | 0.49 | 0.55 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | 0 | 0 | 0 | 2.7 | 0.2 | 0.5 | – | – | – | 0 | 0 | 0 | 0 | -0.5 | 0.3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1.9 | 0.1 | 0.2 | 0.4 | 1.0 | 1.2 |
Endosome | 0.2 | -1.4 | -3.1 | -4.8 | -3.4 | -3.0 | – | – | – | 2.0 | 3.3 | 4.3 | 3.0 | 3.9 | 4.1 |
Golgi | -2.7 | -4.2 | -6.5 | -8.8 | -8.2 | -7.4 | – | – | – | 7.1 | 8.4 | 7.5 | 5.4 | 6.5 | 2.6 |
Mitochondria | 1.2 | 1.1 | 3.0 | 4.2 | 3.7 | 3.3 | – | – | – | -13.0 | -18.4 | -18.0 | -17.7 | -17.5 | -16.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 2.9 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.9 | 0 | 0 | -0.5 | -0.6 | -0.9 | – | – | – | 0 | 0 | 0 | -1.3 | -0.8 | 1.6 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1.7 | – | – | – | 0 | 1.9 | 2.1 | 2.5 | 1.8 | 2.7 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 2.7 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 4.2 | 3.6 | 4.5 | 5.1 | 4.6 | 4.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 1.1 | – | – | – | 4.0 | 5.6 | 3.9 | 6.0 | 4.3 | 6.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0244 | 0.019 | 0.0126 | 0.0013 | 0 | 0.0124 | 0.0437 | 0.0116 | 0.0095 | 0.0155 | 0.0034 | 0.0098 | 0.0164 | 0.0192 | 0.0238 | 0.0034 | 0.0454 | 0.0092 |
Bud | 0.0005 | 0.0005 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0011 | 0.001 | 0.0011 | 0.0008 | 0.0003 | 0.0017 | 0.0018 | 0.0036 | 0.0176 | 0.025 | 0.0004 | 0.0028 |
Bud Neck | 0.0008 | 0.0009 | 0.0016 | 0.0002 | 0.0001 | 0.0006 | 0.0038 | 0.0005 | 0.0012 | 0.001 | 0.0005 | 0.0005 | 0.0006 | 0.0005 | 0.0005 | 0.0005 | 0.0006 | 0.0015 |
Bud Periphery | 0.0005 | 0.0009 | 0.0002 | 0.0003 | 0 | 0.0002 | 0.0011 | 0.0008 | 0.0008 | 0.0008 | 0.0003 | 0.0019 | 0.0013 | 0.0016 | 0.0085 | 0.0036 | 0.0004 | 0.0014 |
Bud Site | 0.0011 | 0.0038 | 0.001 | 0.0002 | 0 | 0.0002 | 0.019 | 0.006 | 0.0123 | 0.003 | 0.001 | 0.0004 | 0.0099 | 0.0172 | 0.0221 | 0.0013 | 0.0007 | 0.0045 |
Cell Periphery | 0.0002 | 0.0019 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0006 | 0.0003 | 0.0001 | 0.0002 |
Cytoplasm | 0.0257 | 0.0082 | 0.0289 | 0.003 | 0.0004 | 0.0013 | 0.022 | 0.0055 | 0.0049 | 0.004 | 0.004 | 0.0129 | 0.0049 | 0.0132 | 0.0076 | 0.0156 | 0.0053 | 0.0106 |
Cytoplasmic Foci | 0.0849 | 0.1082 | 0.0822 | 0.0504 | 0.0821 | 0.0627 | 0.0792 | 0.1129 | 0.0905 | 0.0753 | 0.1165 | 0.0705 | 0.1104 | 0.1352 | 0.1127 | 0.185 | 0.1189 | 0.106 |
Eisosomes | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0132 | 0.0016 | 0.0037 | 0.0008 | 0.0003 | 0.0026 | 0.0057 | 0.0016 | 0.0011 | 0.0026 | 0.0018 | 0.0015 | 0.0022 | 0.0009 | 0.0008 | 0.0007 | 0.0008 | 0.0013 |
Endosome | 0.3209 | 0.2916 | 0.2453 | 0.1659 | 0.6343 | 0.161 | 0.2933 | 0.3179 | 0.3192 | 0.415 | 0.3907 | 0.1988 | 0.2733 | 0.3296 | 0.2214 | 0.2918 | 0.2624 | 0.1839 |
Golgi | 0.4104 | 0.4841 | 0.5533 | 0.5313 | 0.2164 | 0.584 | 0.3597 | 0.4668 | 0.4919 | 0.4236 | 0.4259 | 0.6201 | 0.4914 | 0.4286 | 0.5323 | 0.4209 | 0.5083 | 0.6141 |
Lipid Particles | 0.0157 | 0.0243 | 0.0172 | 0.002 | 0.0535 | 0.024 | 0.0447 | 0.0191 | 0.0105 | 0.0165 | 0.0129 | 0.0175 | 0.0237 | 0.0149 | 0.0034 | 0.01 | 0.0087 | 0.0163 |
Mitochondria | 0.0459 | 0.0298 | 0.0208 | 0.2377 | 0.0017 | 0.1059 | 0.045 | 0.0403 | 0.032 | 0.0162 | 0.0199 | 0.0539 | 0.0307 | 0.0139 | 0.0276 | 0.0064 | 0.0368 | 0.0308 |
None | 0.0073 | 0.002 | 0.0008 | 0.0001 | 0 | 0.0004 | 0.0084 | 0.0001 | 0.0002 | 0.0006 | 0.0004 | 0.0007 | 0.001 | 0.0013 | 0.0025 | 0.0002 | 0.0003 | 0.0036 |
Nuclear Periphery | 0.0212 | 0.0004 | 0.0068 | 0.0001 | 0.0001 | 0.0223 | 0.0183 | 0.0004 | 0.0003 | 0.0049 | 0.002 | 0.0005 | 0.0073 | 0.0004 | 0.0003 | 0.0004 | 0.0006 | 0.0013 |
Nucleolus | 0.0006 | 0.0002 | 0.0001 | 0 | 0 | 0.0002 | 0.0013 | 0.0001 | 0.0003 | 0.0005 | 0.0012 | 0.0001 | 0.0008 | 0.0004 | 0.0008 | 0.0002 | 0.0001 | 0.0001 |
Nucleus | 0.0071 | 0.0002 | 0.0042 | 0.0001 | 0 | 0.0027 | 0.017 | 0.0001 | 0.0004 | 0.0017 | 0.0008 | 0.0001 | 0.0048 | 0.0007 | 0.0008 | 0.0042 | 0.0001 | 0.0005 |
Peroxisomes | 0.0056 | 0.0166 | 0.0175 | 0.0009 | 0.0045 | 0.0132 | 0.0093 | 0.0098 | 0.0155 | 0.0065 | 0.0101 | 0.0045 | 0.0131 | 0.0096 | 0.0119 | 0.0213 | 0.0061 | 0.0074 |
Punctate Nuclear | 0.0058 | 0.0003 | 0.0009 | 0 | 0.0001 | 0.0006 | 0.0101 | 0.0002 | 0.0021 | 0.0027 | 0.0007 | 0.0002 | 0.0007 | 0.0022 | 0.0012 | 0.0025 | 0.0005 | 0.0008 |
Vacuole | 0.0035 | 0.003 | 0.001 | 0.002 | 0.0037 | 0.0019 | 0.0068 | 0.0026 | 0.003 | 0.0042 | 0.0045 | 0.0016 | 0.0025 | 0.0041 | 0.0019 | 0.0037 | 0.0016 | 0.0017 |
Vacuole Periphery | 0.0044 | 0.0022 | 0.0014 | 0.0032 | 0.0025 | 0.0038 | 0.0096 | 0.0024 | 0.003 | 0.0045 | 0.0029 | 0.0026 | 0.0029 | 0.0025 | 0.0016 | 0.003 | 0.0017 | 0.0019 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.5982 | 12.5853 | 10.5062 | 19.8816 | 12.5492 | 6.1559 | 13.5203 | 15.8351 | 15.8311 | 18.9928 |
Translational Efficiency | 0.7615 | 1.0233 | 1.056 | 0.6112 | 1.0046 | 2.2843 | 1.0897 | 0.9326 | 0.9128 | 0.9552 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1272 | 132 | 1273 | 1067 | 1058 | 1713 | 1257 | 910 | 2330 | 1845 | 2530 | 1977 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 767.91 | 1013.00 | 944.97 | 967.53 | 843.94 | 897.47 | 995.05 | 997.11 | 802.43 | 905.74 | 969.85 | 981.15 |
Standard Deviation | 118.43 | 134.20 | 131.81 | 133.74 | 125.60 | 115.85 | 137.54 | 140.36 | 127.49 | 120.98 | 137.00 | 137.62 |
Intensity Change Log 2 | — | 0.399625 | 0.299331 | 0.333369 | — | 0.088723 | 0.237629 | 0.240612 | — | 0.245210 | 0.267354 | 0.285547 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002906 | 0.002812 | 0.001605 | 0.019323 | 0.001738 | 0.001160 | 0.002758 | 0.012531 | 0.002376 | 0.001278 | 0.002178 | 0.016197 |
Bud Neck | 0.008646 | 0.000068 | 0.000541 | 0.000515 | 0.000142 | 0.000113 | 0.000916 | 0.000783 | 0.004785 | 0.000109 | 0.000727 | 0.000638 |
Bud Site | 0.011411 | 0.000814 | 0.002012 | 0.012985 | 0.000862 | 0.000977 | 0.004206 | 0.009318 | 0.006621 | 0.000966 | 0.003102 | 0.011297 |
Cell Periphery | 0.000448 | 0.000063 | 0.000059 | 0.000185 | 0.000074 | 0.000058 | 0.000063 | 0.000098 | 0.000279 | 0.000058 | 0.000061 | 0.000145 |
Cytoplasm | 0.144959* | 0.000445 | 0.001178 | 0.004242 | 0.001463 | 0.000500 | 0.001982 | 0.002932 | 0.079801 | 0.000496 | 0.001578 | 0.003639 |
Cytoplasmic Foci | 0.212411* | 0.173095 | 0.208759* | 0.184915* | 0.220920* | 0.166000 | 0.253914* | 0.209163* | 0.216275* | 0.166508 | 0.231194* | 0.196076* |
Eisosomes | 0.000390 | 0.000042 | 0.000032 | 0.000103 | 0.000053 | 0.000033 | 0.000043 | 0.000072 | 0.000237 | 0.000034 | 0.000037 | 0.000089 |
Endoplasmic Reticulum | 0.008720 | 0.000248 | 0.002056 | 0.002749 | 0.001074 | 0.000378 | 0.001981 | 0.001378 | 0.005248 | 0.000369 | 0.002019 | 0.002118 |
Endosome | 0.095972 | 0.137991 | 0.235263* | 0.176830 | 0.086947 | 0.146351 | 0.228028* | 0.224574* | 0.091874 | 0.145753 | 0.231668* | 0.198806 |
Golgi | 0.318283* | 0.641863* | 0.442689* | 0.524993* | 0.593730* | 0.624935* | 0.333697* | 0.459893* | 0.443357* | 0.626147* | 0.388538* | 0.495028* |
Lipid Particles | 0.053606 | 0.000397 | 0.025941 | 0.002457 | 0.013993 | 0.002791 | 0.044581 | 0.002746 | 0.035619 | 0.002620 | 0.035202 | 0.002590 |
Mitochondria | 0.057329 | 0.019507 | 0.009765 | 0.013844 | 0.050693 | 0.032792 | 0.012124 | 0.009142 | 0.054315 | 0.031841 | 0.010937 | 0.011680 |
Mitotic Spindle | 0.007325 | 0.001615 | 0.014038 | 0.010809 | 0.007561 | 0.001203 | 0.045685 | 0.018512 | 0.007432 | 0.001233 | 0.029761 | 0.014354 |
None | 0.002735 | 0.000014 | 0.000051 | 0.000038 | 0.000066 | 0.000593 | 0.000556 | 0.000036 | 0.001523 | 0.000552 | 0.000302 | 0.000037 |
Nuclear Periphery | 0.000652 | 0.000031 | 0.000445 | 0.000350 | 0.000160 | 0.000047 | 0.001077 | 0.000235 | 0.000429 | 0.000046 | 0.000759 | 0.000297 |
Nuclear Periphery Foci | 0.001537 | 0.000083 | 0.004928 | 0.000911 | 0.001564 | 0.000144 | 0.012078 | 0.000937 | 0.001550 | 0.000140 | 0.008480 | 0.000923 |
Nucleolus | 0.000745 | 0.000001 | 0.000923 | 0.000014 | 0.000034 | 0.000007 | 0.002182 | 0.000044 | 0.000422 | 0.000006 | 0.001548 | 0.000028 |
Nucleus | 0.028228 | 0.000004 | 0.000386 | 0.000042 | 0.000119 | 0.000012 | 0.000830 | 0.000105 | 0.015465 | 0.000011 | 0.000607 | 0.000071 |
Peroxisomes | 0.015336 | 0.002845 | 0.002822 | 0.004644 | 0.002981 | 0.002168 | 0.007449 | 0.008347 | 0.009726 | 0.002217 | 0.005121 | 0.006349 |
Vacuole | 0.022228 | 0.006574 | 0.028623 | 0.027441 | 0.005166 | 0.007055 | 0.033604 | 0.027418 | 0.014481 | 0.007021 | 0.031098 | 0.027430 |
Vacuole Periphery | 0.006131 | 0.011489 | 0.017884 | 0.012610 | 0.010659 | 0.012681 | 0.012246 | 0.011735 | 0.008187 | 0.012596 | 0.015083 | 0.012207 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.05 | 1.96 | -6.30 | -5.05 | -6.73 | 1.23 | -1.41 | -4.57 | -4.88 | -4.09 | 2.95 | 0.43 | -7.77 | -8.42 | -7.80 |
Bud Neck | 9.15 | 8.50 | 8.61 | -5.47 | 0.19 | 0.68 | -5.12 | -3.74 | -3.96 | 0.61 | 8.99 | 7.60 | 7.82 | -5.86 | 0.68 |
Bud Site | 9.39 | 8.16 | -1.06 | -9.78 | -8.69 | -0.52 | -5.61 | -6.99 | -6.90 | -3.85 | 8.86 | 4.97 | -4.49 | -11.77 | -8.88 |
Cell Periphery | 12.21 | 12.86 | 7.17 | -5.16 | -5.75 | 1.50 | 1.37 | -1.57 | -2.46 | -2.40 | 11.57 | 12.52 | 6.22 | -5.53 | -6.16 |
Cytoplasm | 23.31 | 22.95 | 22.59 | -7.88 | -3.13 | 2.53 | -1.23 | -2.45 | -5.29 | -1.73 | 21.45 | 20.96 | 20.47 | -9.38 | -3.66 |
Cytoplasmic Foci | 3.49 | 0.75 | 4.73 | -1.03 | 4.20 | 10.55 | -5.17 | 1.74 | -7.09 | 6.30 | 11.76 | -3.22 | 4.59 | -6.76 | 7.76 |
Eisosomes | 6.16 | 6.39 | 5.05 | -5.41 | -7.74 | 5.48 | 2.39 | -1.50 | -3.24 | -2.31 | 6.59 | 6.46 | 4.69 | -7.32 | -6.83 |
Endoplasmic Reticulum | 6.95 | 4.84 | 4.56 | -5.27 | -0.89 | 4.73 | -3.79 | -0.67 | -2.16 | 1.19 | 7.22 | 4.27 | 4.14 | -5.17 | -0.21 |
Endosome | -4.28 | -22.82 | -14.79 | -3.71 | 8.46 | -15.59 | -24.22 | -20.98 | -11.54 | 0.38 | -15.57 | -32.93* | -25.11 | -11.29 | 6.18 |
Golgi | -16.81 | -10.80 | -17.46 | 6.35 | -7.84 | -3.69 | 26.64 | 12.16 | 16.37 | -11.24 | -22.52 | 6.61 | -5.90 | 17.48 | -13.74 |
Lipid Particles | 15.71 | 6.13 | 14.97 | -4.63 | 7.77 | 5.55 | -7.33 | 5.60 | 0.03 | 11.13 | 15.37 | 0.05 | 15.62 | 0.01 | 13.51 |
Mitochondria | 4.53 | 12.17 | 10.70 | 0.74 | -1.86 | 3.63 | 8.61 | 9.34 | 8.19 | 1.48 | 6.08 | 14.70 | 14.21 | 7.56 | -0.48 |
Mitotic Spindle | 4.15 | -2.37 | -1.46 | -4.86 | 0.88 | 5.73 | -9.82 | -3.46 | -5.69 | 5.56 | 7.04 | -9.38 | -3.57 | -7.68 | 5.35 |
None | 5.12 | 5.04 | 5.07 | -3.75 | 1.16 | -0.93 | -1.51 | 1.99 | 0.98 | 1.59 | 1.63 | 3.59 | 5.09 | 0.97 | 1.64 |
Nuclear Periphery | 6.97 | 1.72 | 1.21 | -1.43 | 0.35 | 2.57 | -6.51 | -1.16 | -3.63 | 5.86 | 7.13 | -3.43 | 0.95 | -2.03 | 3.07 |
Nuclear Periphery Foci | 7.56 | -3.38 | 1.90 | -2.95 | 3.92 | 2.03 | -6.23 | 0.86 | -4.46 | 7.16 | 4.24 | -7.12 | 1.69 | -4.49 | 8.09 |
Nucleolus | 7.07 | -0.46 | 6.92 | -1.98 | 1.71 | 3.09 | -3.05 | -0.75 | -2.76 | 3.04 | 7.16 | -2.66 | 6.72 | -3.00 | 3.46 |
Nucleus | 13.47 | 13.22 | 13.45 | -3.77 | 2.19 | 2.03 | -2.96 | 0.12 | -1.54 | 2.98 | 13.10 | 12.44 | 13.04 | -2.15 | 3.72 |
Peroxisomes | 6.95 | 9.08 | 7.23 | -1.28 | -2.17 | 1.36 | -4.55 | -4.00 | -4.53 | -0.53 | 8.69 | 5.00 | 3.24 | -5.00 | -1.36 |
Vacuole | 5.87 | -2.55 | -1.97 | -7.10 | 0.24 | -2.65 | -13.90 | -9.01 | -8.24 | 1.92 | 6.72 | -10.01 | -6.86 | -12.07 | 1.58 |
Vacuole Periphery | -1.47 | -7.86 | -5.04 | -0.30 | 2.76 | -1.40 | -1.08 | -0.62 | 0.59 | 0.30 | -4.20 | -6.79 | -3.84 | 0.29 | 2.29 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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