Standard name | |
---|---|
Human Ortholog | |
Description | Hexokinase isoenzyme 2; phosphorylates glucose in cytosol; predominant hexokinase during growth on glucose; represses expression of HXK1, GLK1, induces expression of its own gene; antiapoptotic; phosphorylation/dephosphorylation at Ser14 by kinase Snf1p, phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; functions downstream of Sit4p in control of cell cycle, mitochondrial function, oxidative stress resistance, chronological lifespan; has paralog HXK1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | – | 2 | 8 | 8 | 4 | 6 | 2 | 3 | 0 | 1 | 3 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 289 | 50 | 62 | 40 | – | 136 | 167 | 230 | 148 | 149 | 108 | 115 | 146 | 245 | 274 | 332 | 196 | 184 | 262 | 68 | 60 | 67 | 139 | 130 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 3 | 4 | 4 | 5 | 1 | 6 | 0 | 0 | 1 | 14 | 22 | 14 | 1 | 0 | 0 | 1 | 0 | 0 |
Endosome | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Unique Cell Count | 291 | 50 | 62 | 40 | 137 | 174 | 237 | 154 | 157 | 112 | 122 | 146 | 245 | 277 | 338 | 196 | 186 | 272 | 73 | 73 | 73 | 145 | 139 | |
Labelled Cell Count | 292 | 50 | 62 | 40 | 138 | 180 | 242 | 157 | 162 | 113 | 124 | 146 | 247 | 279 | 354 | 221 | 199 | 272 | 73 | 73 | 73 | 145 | 139 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 248.4 | 251.1 | 213.4 | 251.6 | – | 240.6 | 206.1 | 203.1 | 200.6 | 171.4 | 172.6 | 169.0 | 303.3 | 308.8 | 301.2 | 283.7 | 299.6 | 364.6 | 251.9 | 274.6 | 302.3 |
Std Deviation (1e-4) | 45.4 | 40.5 | 100.0 | 80.4 | – | 65.7 | 63.3 | 64.9 | 71.1 | 55.8 | 69.1 | 50.6 | 53.8 | 62.4 | 60.5 | 106.5 | 104.7 | 117.0 | 68.8 | 55.7 | 85.8 |
Intensity Change (Log2) | – | – | – | 0.24 | – | 0.17 | -0.05 | -0.07 | -0.09 | -0.32 | -0.31 | -0.34 | 0.51 | 0.53 | 0.5 | 0.41 | 0.49 | 0.77 | 0.24 | 0.36 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | – | -0.7 | -1.6 | -1.4 | -1.6 | -1.8 | -1.5 | -1.9 | 0 | 0 | -0.8 | -1.1 | 0 | -0.8 |
Endoplasmic Reticulum | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 |
Endosome | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 212.8998 | 280.5663 | 257.5899 | 239.0329 | 216.8561 | 246.1902 | 193.8577 | 251.1899 | 228.4774 | 197.8829 | 190.8985 | 163.9085 | 208.2498 | 271.7524 | 244.2747 | 227.8459 | 188.0332 | 231.8168 |
Actin | 0.0059 | 0.0001 | 0.0001 | 0 | 0.0055 | 0.0002 | 0.0025 | 0.0001 | 0.003 | 0.0005 | 0.0027 | 0.0006 | 0.0082 | 0.0001 | 0.008 | 0.0007 | 0.024 | 0.0001 |
Bud | 0.0024 | 0 | 0.0015 | 0.0001 | 0.0017 | 0.0002 | 0.0005 | 0 | 0.0011 | 0.0001 | 0.001 | 0.0002 | 0.0004 | 0 | 0.0005 | 0.0006 | 0.0008 | 0.0001 |
Bud Neck | 0.0006 | 0.0002 | 0.0003 | 0.0004 | 0.0009 | 0.0018 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0.0006 | 0.0008 | 0.002 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0002 |
Bud Periphery | 0.0013 | 0 | 0.0006 | 0 | 0.0015 | 0 | 0.0005 | 0 | 0.0006 | 0.0001 | 0.0006 | 0 | 0.0005 | 0 | 0.0009 | 0.0003 | 0.0013 | 0 |
Bud Site | 0.0012 | 0 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0003 | 0 | 0.0011 | 0.0001 | 0.0021 | 0.0001 | 0.0014 | 0 | 0.0006 | 0.0002 | 0.0041 | 0 |
Cell Periphery | 0.0021 | 0.0001 | 0.0004 | 0.0031 | 0.0047 | 0.0001 | 0.0004 | 0 | 0.0008 | 0.0001 | 0.0013 | 0.0001 | 0.0012 | 0 | 0.0004 | 0.0002 | 0.0012 | 0 |
Cytoplasm | 0.9167 | 0.9975 | 0.9639 | 0.954 | 0.8459 | 0.9912 | 0.9322 | 0.9989 | 0.9648 | 0.9457 | 0.8746 | 0.9531 | 0.881 | 0.9941 | 0.9171 | 0.934 | 0.7696 | 0.7969 |
Cytoplasmic Foci | 0.0076 | 0 | 0.0046 | 0.0143 | 0.0041 | 0.0003 | 0.0048 | 0 | 0.001 | 0.0003 | 0.0048 | 0.0018 | 0.0298 | 0.0001 | 0.011 | 0.0042 | 0.0044 | 0.1416 |
Eisosomes | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.002 | 0.0001 | 0.0023 | 0 | 0.0006 | 0 | 0.0006 | 0.0001 | 0.0009 | 0 |
Endoplasmic Reticulum | 0.0009 | 0.0001 | 0.0002 | 0.0003 | 0.0025 | 0.0003 | 0.0016 | 0 | 0.0008 | 0.0009 | 0.003 | 0.001 | 0.0015 | 0.0006 | 0.0003 | 0.001 | 0.0006 | 0.0001 |
Endosome | 0.0026 | 0 | 0.001 | 0.0001 | 0.0434 | 0.0002 | 0.0013 | 0 | 0.0009 | 0.0001 | 0.006 | 0.0019 | 0.0027 | 0 | 0.0047 | 0.0003 | 0.0091 | 0.0004 |
Golgi | 0.0012 | 0 | 0.0005 | 0 | 0.0218 | 0 | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0006 | 0 | 0.0012 | 0 | 0.0042 | 0.0001 | 0.0059 | 0.0003 |
Lipid Particles | 0.0019 | 0 | 0.0043 | 0.0026 | 0.0047 | 0 | 0.0003 | 0 | 0.0007 | 0 | 0.0022 | 0 | 0.0057 | 0 | 0.0044 | 0.0006 | 0.0073 | 0.0266 |
Mitochondria | 0.0074 | 0 | 0.0033 | 0.0027 | 0.0143 | 0 | 0.0025 | 0 | 0.0061 | 0.0455 | 0.0516 | 0.0004 | 0.0077 | 0 | 0.0162 | 0.0165 | 0.121 | 0.0019 |
None | 0.006 | 0.0001 | 0.0047 | 0.002 | 0.0064 | 0.0001 | 0.0078 | 0.0001 | 0.0015 | 0.0008 | 0.0136 | 0.0016 | 0.0105 | 0.0034 | 0.0059 | 0.0061 | 0.0039 | 0.0023 |
Nuclear Periphery | 0.0051 | 0.0004 | 0.001 | 0.0015 | 0.0089 | 0.0015 | 0.0213 | 0.0001 | 0.0019 | 0.001 | 0.0073 | 0.0093 | 0.0062 | 0.0003 | 0.001 | 0.0042 | 0.0023 | 0.0005 |
Nucleolus | 0.0097 | 0 | 0.0041 | 0.0075 | 0.0018 | 0 | 0.0035 | 0 | 0.0014 | 0.0001 | 0.0035 | 0.0002 | 0.014 | 0 | 0.0114 | 0.0074 | 0.0178 | 0.0064 |
Nucleus | 0.0118 | 0.0011 | 0.0054 | 0.0046 | 0.0098 | 0.0024 | 0.0126 | 0.0006 | 0.0036 | 0.0026 | 0.005 | 0.0259 | 0.0088 | 0.001 | 0.0035 | 0.017 | 0.0053 | 0.0019 |
Peroxisomes | 0.0017 | 0 | 0.0007 | 0.0003 | 0.0006 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0006 | 0 | 0.0069 | 0 | 0.0047 | 0.0006 | 0.0067 | 0.0016 |
Punctate Nuclear | 0.0025 | 0 | 0.0004 | 0.0002 | 0.0005 | 0 | 0.0015 | 0 | 0.0003 | 0.0001 | 0.0006 | 0.0011 | 0.0023 | 0 | 0.0013 | 0.0009 | 0.0004 | 0.0175 |
Vacuole | 0.0075 | 0.0004 | 0.0021 | 0.0055 | 0.0149 | 0.0014 | 0.0028 | 0 | 0.0042 | 0.0005 | 0.0088 | 0.0013 | 0.0039 | 0.0001 | 0.0013 | 0.0021 | 0.0051 | 0.0007 |
Vacuole Periphery | 0.0033 | 0 | 0.0007 | 0.0006 | 0.0056 | 0.0003 | 0.0029 | 0 | 0.0034 | 0.0009 | 0.0072 | 0.0006 | 0.0036 | 0 | 0.0017 | 0.0027 | 0.0077 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1179.306 | 998.5287 | 894.7526 | 920.5084 | 981.159 | 1299.8019 | 1408.4385 | 1287.0491 | 1236.8775 | 1255.4012 |
Translational Efficiency | 1.3529 | 1.5353 | 1.4036 | 1.5318 | 1.4397 | 1.5087 | 1.571 | 1.4082 | 1.5082 | 1.4768 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1976 | 1511 | 94 | 1421 | 2246 | 859 | 414 | 1620 | 4222 | 2370 | 508 | 3041 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 13332.05 | 14938.07 | 21799.99 | 21596.39 | 15605.60 | 13778.23 | 23910.90 | 29825.95 | 14541.52 | 14517.69 | 23520.30 | 25980.44 |
Standard Deviation | 2869.04 | 3366.74 | 4286.99 | 5776.80 | 3133.05 | 3533.47 | 4149.82 | 6941.23 | 3218.90 | 3473.15 | 4255.25 | 7623.62 |
Intensity Change Log 2 | — | 0.164095 | 0.709429 | 0.695892 | — | -0.179673 | 0.615605 | 0.934504 | — | -0.011078 | 0.659590 | 0.829447 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000020 | 0.000067 | 0.000193 | 0.000171 | 0.000014 | 0.000082 | 0.000077 | 0.000033 | 0.000017 | 0.000073 | 0.000099 | 0.000098 |
Bud Neck | 0.000100 | 0.000320 | 0.004500 | 0.001297 | 0.000087 | 0.000613 | 0.002425 | 0.000724 | 0.000093 | 0.000426 | 0.002809 | 0.000992 |
Bud Site | 0.000592 | 0.001198 | 0.000839 | 0.005306 | 0.000294 | 0.001680 | 0.001431 | 0.007483 | 0.000434 | 0.001373 | 0.001321 | 0.006466 |
Cell Periphery | 0.003114 | 0.002967 | 0.023616 | 0.026365 | 0.001101 | 0.008147 | 0.012856 | 0.002134 | 0.002043 | 0.004844 | 0.014847 | 0.013457 |
Cytoplasm | 0.926741* | 0.900930* | 0.523199* | 0.738139* | 0.916346* | 0.837534* | 0.709203* | 0.868178* | 0.921211* | 0.877953* | 0.674785* | 0.807413* |
Cytoplasmic Foci | 0.002664 | 0.003441 | 0.049387 | 0.004506 | 0.001361 | 0.008653 | 0.012779 | 0.002425 | 0.001971 | 0.005330 | 0.019553 | 0.003397 |
Eisosomes | 0.000000 | 0.000001 | 0.000011 | 0.000004 | 0.000000 | 0.000002 | 0.000004 | 0.000001 | 0.000000 | 0.000001 | 0.000005 | 0.000002 |
Endoplasmic Reticulum | 0.000017 | 0.000029 | 0.000055 | 0.000070 | 0.000014 | 0.000164 | 0.000033 | 0.000061 | 0.000015 | 0.000078 | 0.000037 | 0.000065 |
Endosome | 0.000026 | 0.000036 | 0.000187 | 0.000372 | 0.000013 | 0.000228 | 0.000149 | 0.000325 | 0.000019 | 0.000106 | 0.000156 | 0.000347 |
Golgi | 0.002739 | 0.001768 | 0.011841 | 0.002714 | 0.001131 | 0.002529 | 0.006030 | 0.000867 | 0.001883 | 0.002044 | 0.007105 | 0.001730 |
Lipid Particles | 0.000932 | 0.001761 | 0.011780 | 0.015131 | 0.000414 | 0.004254 | 0.008154 | 0.000568 | 0.000656 | 0.002664 | 0.008825 | 0.007373 |
Mitochondria | 0.000086 | 0.000257 | 0.000285 | 0.000255 | 0.000065 | 0.000137 | 0.000098 | 0.000138 | 0.000075 | 0.000213 | 0.000133 | 0.000193 |
Mitotic Spindle | 0.000153 | 0.000232 | 0.000381 | 0.000865 | 0.000088 | 0.000301 | 0.000397 | 0.000038 | 0.000118 | 0.000257 | 0.000394 | 0.000425 |
None | 0.011246 | 0.012059 | 0.003275 | 0.006141 | 0.011119 | 0.013580 | 0.002545 | 0.001693 | 0.011179 | 0.012610 | 0.002680 | 0.003772 |
Nuclear Periphery | 0.000099 | 0.000147 | 0.004820 | 0.000309 | 0.000065 | 0.000297 | 0.001243 | 0.000008 | 0.000081 | 0.000201 | 0.001905 | 0.000149 |
Nuclear Periphery Foci | 0.001634 | 0.001754 | 0.006398 | 0.002359 | 0.000630 | 0.002441 | 0.004665 | 0.000101 | 0.001100 | 0.002003 | 0.004986 | 0.001156 |
Nucleolus | 0.000033 | 0.000049 | 0.000152 | 0.000181 | 0.000025 | 0.000169 | 0.000116 | 0.000156 | 0.000029 | 0.000093 | 0.000123 | 0.000168 |
Nucleus | 0.042215 | 0.059674 | 0.229178* | 0.035573 | 0.061926 | 0.089609 | 0.096719 | 0.004205 | 0.052701 | 0.070524 | 0.121229 | 0.018862 |
Peroxisomes | 0.000003 | 0.000010 | 0.000007 | 0.000069 | 0.000003 | 0.000018 | 0.000011 | 0.000229 | 0.000003 | 0.000013 | 0.000010 | 0.000154 |
Vacuole | 0.005356 | 0.012013 | 0.119266 | 0.155415 | 0.004277 | 0.027893 | 0.134142 | 0.103282 | 0.004782 | 0.017768 | 0.131389 | 0.127643 |
Vacuole Periphery | 0.002230 | 0.001289 | 0.010630 | 0.004757 | 0.001028 | 0.001668 | 0.006921 | 0.007350 | 0.001591 | 0.001426 | 0.007607 | 0.006138 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.21 | -7.51 | -8.42 | -3.16 | 1.37 | -7.40 | -13.20 | -7.41 | 5.80 | 10.30 | -3.96 | -14.09 | -9.26 | -0.81 | 2.18 |
Bud Neck | -6.06 | -6.23 | -14.59 | -11.22 | 4.43 | -4.38 | -9.86 | -8.37 | 1.17 | 8.30 | -6.54 | -11.61 | -16.59 | -6.41 | 8.49 |
Bud Site | -2.23 | -2.52 | -10.57 | -7.04 | -8.18 | -5.46 | -5.09 | -15.61 | -10.30 | -11.08 | -4.43 | -5.18 | -18.61 | -11.99 | -12.80 |
Cell Periphery | -0.11 | -7.84 | -13.09 | -13.12 | 0.24 | -8.63 | -7.55 | -2.94 | 7.55 | 6.97 | -7.28 | -9.35 | -12.98 | -8.14 | 2.01 |
Cytoplasm | 7.90 | 15.99 | 26.72 | 21.48 | -8.42 | 13.60 | 19.56 | 8.14 | -5.91 | -15.64 | 14.40 | 24.17 | 24.93 | 12.21 | -14.03 |
Cytoplasmic Foci | -2.91 | -7.67 | -2.86 | -1.38 | 7.29 | -9.99 | -9.44 | -3.09 | 9.12 | 9.02 | -9.81 | -11.19 | -3.63 | 4.20 | 10.27 |
Eisosomes | -5.01 | -7.50 | -12.13 | -10.08 | 5.05 | -8.19 | -10.11 | -9.99 | 6.31 | 9.15 | -9.73 | -12.29 | -13.61 | -4.12 | 8.43 |
Endoplasmic Reticulum | -2.00 | -3.62 | -5.72 | -3.56 | -0.43 | -1.15 | -4.77 | -5.54 | 0.77 | -3.01 | -1.35 | -6.51 | -7.95 | 0.23 | -3.50 |
Endosome | -2.70 | -6.04 | -1.77 | -1.68 | -0.59 | -1.47 | -7.53 | -11.19 | -0.75 | -3.60 | -1.71 | -8.88 | -3.69 | -2.30 | -1.51 |
Golgi | 3.16 | -5.47 | 0.57 | -2.45 | 5.59 | -4.27 | -8.92 | 2.11 | 5.64 | 9.82 | -0.95 | -9.31 | 1.45 | 2.31 | 9.86 |
Lipid Particles | -3.37 | -6.04 | -12.96 | -11.50 | -0.49 | -6.54 | -9.75 | -0.93 | 6.42 | 9.67 | -7.42 | -11.22 | -12.58 | -6.85 | 3.81 |
Mitochondria | -0.99 | -5.15 | -3.66 | 0.06 | 0.24 | -3.50 | -6.07 | -4.17 | 0.44 | -0.94 | -1.22 | -6.58 | -4.79 | 0.28 | -1.88 |
Mitotic Spindle | -1.10 | -2.51 | -2.21 | -2.00 | -1.56 | -1.75 | -2.83 | 2.69 | 2.22 | 3.33 | -2.18 | -3.10 | -2.05 | -1.30 | -0.44 |
None | -0.32 | 9.28 | 4.43 | 4.36 | -2.30 | -1.17 | 13.44 | 11.11 | 7.65 | 0.98 | -0.98 | 16.28 | 10.09 | 8.79 | -1.73 |
Nuclear Periphery | -3.47 | -6.06 | -6.78 | -4.89 | 5.73 | -5.94 | -8.85 | 9.28 | 7.46 | 9.21 | -7.16 | -10.09 | -4.80 | 1.92 | 9.64 |
Nuclear Periphery Foci | -0.73 | -4.37 | -2.38 | -2.24 | 3.73 | -5.83 | -8.30 | 8.77 | 7.63 | 9.37 | -4.52 | -8.52 | -0.62 | 4.80 | 8.56 |
Nucleolus | -3.15 | -6.37 | -7.48 | -6.63 | -0.17 | -2.22 | -9.90 | -18.19 | 0.20 | -1.23 | -2.74 | -11.43 | -13.33 | -3.14 | -1.73 |
Nucleus | -7.81 | -10.32 | -1.05 | 5.83 | 10.15 | -7.71 | -8.49 | 32.89 | 23.63 | 18.23 | -9.27 | -13.13 | 18.79 | 22.51 | 17.61 |
Peroxisomes | -2.51 | -4.96 | -5.88 | -4.93 | -5.22 | -1.82 | -2.14 | -12.22 | -10.48 | -11.39 | -2.89 | -2.51 | -13.44 | -11.94 | -12.00 |
Vacuole | -6.02 | -6.99 | -27.14 | -25.20 | -1.79 | -8.72 | -14.64 | -20.84 | -11.58 | 5.79 | -10.64 | -16.11 | -33.71 | -27.36 | 3.09 |
Vacuole Periphery | 1.35 | -3.40 | -3.33 | -5.30 | 2.50 | -1.20 | -5.24 | -9.66 | -8.35 | -1.05 | 0.42 | -5.79 | -9.46 | -9.57 | 1.03 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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