Standard name | |
---|---|
Human Ortholog | |
Description | S-adenosylmethionine-dependent lysine methyltransferase; involved in the trimethylation of eEF1A (Tef1p/Tef2p) at lysine 79; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in budding yeast; expresses a circular RNA; originally misclassified as a N-6-adenine specific DNA methyltransferase based on sequence similarity; both Efm5p and human ortholog N6AMT2 can methylate eEF1a from either species in vitro |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 1 | 5 | 2 | 4 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 4 | 0 | 3 | 5 | 10 | 7 | 10 | 10 | 14 | 4 | 9 | – | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 198 | 390 | 231 | 204 | 262 | 311 | 324 | 369 | 241 | 256 | 243 | 238 | – | 124 | 67 | 257 | 186 | 203 | 194 | 372 | 221 | 43 | 77 | 116 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 3 | 9 | 23 | 23 | 15 | 18 | 8 | 13 | – | 2 | 0 | 8 | 8 | 6 | 0 | 2 | 1 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 3 | 24 | 6 | 12 | 29 | – | 0 | 0 | 2 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 0 |
Nucleus | 2 | 1 | 0 | 5 | 4 | 5 | 9 | 18 | 3 | 5 | 5 | 7 | – | 0 | 1 | 5 | 1 | 1 | 1 | 8 | 2 | 1 | 4 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 2 | 2 | 2 | 2 | 8 | 14 | 32 | 20 | 15 | 20 | 23 | – | 3 | 0 | 1 | 8 | 9 | 0 | 9 | 1 | 2 | 3 | 3 |
Unique Cell Count | 199 | 391 | 233 | 210 | 266 | 332 | 368 | 438 | 297 | 300 | 285 | 304 | 127 | 68 | 261 | 191 | 209 | 198 | 403 | 230 | 52 | 90 | 131 | |
Labelled Cell Count | 203 | 399 | 235 | 214 | 276 | 345 | 380 | 460 | 315 | 319 | 294 | 323 | 130 | 69 | 275 | 206 | 223 | 198 | 403 | 230 | 52 | 90 | 131 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 27.1 | 14.4 | 21.6 | 20.1 | 19.6 | 15.8 | 14.8 | 14.3 | 12.6 | 13.6 | 13.1 | 13.3 | – | 20.3 | 21.4 | 17.1 | 20.1 | 21.4 | 17.0 | 20.4 | 21.7 |
Std Deviation (1e-4) | 4.3 | 3.7 | 3.7 | 3.7 | 3.3 | 3.2 | 3.0 | 2.7 | 3.1 | 2.9 | 3.2 | 3.1 | – | 4.9 | 4.3 | 4.3 | 5.0 | 5.5 | 4.3 | 6.3 | 6.9 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.14 | -0.45 | -0.54 | -0.59 | -0.78 | -0.67 | -0.72 | -0.71 | – | -0.09 | -0.02 | -0.34 | -0.1 | -0.02 | -0.35 | -0.08 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.6 | -0.7 | -3.2 | -5.0 | -6.0 | -6.6 | -5.6 | -5.6 | -7.2 | – | -1.2 | -0.4 | -0.7 | -1.4 | -1.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 3.6 | 3.2 | 3.1 | 3.4 | 0 | 2.8 | – | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.4 | 0 | 3.2 | 4.8 | – | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 2.2 | 3.6 | 3.4 | 2.7 | 3.5 | 3.7 | – | 0 | 0 | 0 | 0 | 2.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 16.6467 | 20.2234 | 19.0283 | 16.8343 | 17.1273 | 18.6705 | 15.0709 | 19.2399 | 17.0171 | 17.1778 | 15.6203 | 17.1296 | 13.4839 | 18.2349 | 15.4293 | 15.075 | 13.4337 | 16.1586 |
Actin | 0.0162 | 0.0002 | 0.0007 | 0.0142 | 0.0131 | 0.0003 | 0.0111 | 0.0014 | 0.0026 | 0.0003 | 0.0002 | 0.0047 | 0.0083 | 0.0002 | 0.0024 | 0.0012 | 0.0074 | 0.0055 |
Bud | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0001 |
Bud Neck | 0.0009 | 0.0001 | 0.0003 | 0.001 | 0.0008 | 0.0008 | 0.0008 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0009 | 0.0006 | 0.0001 | 0.0004 | 0.0003 | 0.0007 | 0.0007 |
Bud Periphery | 0.0008 | 0 | 0 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0 | 0.0005 | 0.0004 | 0 | 0.0001 | 0 | 0.0008 | 0 |
Bud Site | 0.0014 | 0.0001 | 0.0001 | 0.0008 | 0.0003 | 0 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0018 | 0.0005 | 0.0001 | 0.0023 | 0.0001 | 0.0055 | 0 |
Cell Periphery | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0011 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 |
Cytoplasm | 0.8996 | 0.9959 | 0.9693 | 0.8935 | 0.8656 | 0.9846 | 0.8687 | 0.985 | 0.9635 | 0.9908 | 0.963 | 0.9729 | 0.9341 | 0.9968 | 0.952 | 0.9093 | 0.8006 | 0.9825 |
Cytoplasmic Foci | 0.0086 | 0.0005 | 0.0102 | 0.0123 | 0.0125 | 0.0015 | 0.0181 | 0.0022 | 0.0074 | 0.0017 | 0.0091 | 0.0048 | 0.0043 | 0.0004 | 0.0075 | 0.0075 | 0.0428 | 0.0014 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.008 | 0.0004 | 0.001 | 0.002 | 0.0062 | 0.003 | 0.0072 | 0.0024 | 0.0027 | 0.002 | 0.0027 | 0.002 | 0.0056 | 0.0003 | 0.0014 | 0.0013 | 0.0046 | 0.0012 |
Endosome | 0.0191 | 0.0002 | 0.0078 | 0.0269 | 0.0555 | 0.0011 | 0.0309 | 0.0038 | 0.0108 | 0.0012 | 0.0111 | 0.0044 | 0.0099 | 0.0001 | 0.0115 | 0.02 | 0.0309 | 0.002 |
Golgi | 0.0045 | 0 | 0.0006 | 0.0031 | 0.0047 | 0.0001 | 0.0064 | 0.0009 | 0.0022 | 0.0001 | 0.0003 | 0.0009 | 0.0013 | 0 | 0.0021 | 0.0011 | 0.0178 | 0.0006 |
Lipid Particles | 0.0067 | 0 | 0.0004 | 0.0141 | 0.0049 | 0 | 0.0043 | 0 | 0.0019 | 0 | 0.0008 | 0.0005 | 0.0006 | 0 | 0.0034 | 0.0142 | 0.0283 | 0.0002 |
Mitochondria | 0.0073 | 0 | 0.0005 | 0.002 | 0.002 | 0.0002 | 0.0016 | 0.0004 | 0.0016 | 0.0001 | 0.0001 | 0.0006 | 0.0016 | 0 | 0.001 | 0.0006 | 0.0078 | 0.0002 |
None | 0.0023 | 0.0003 | 0.0005 | 0.0007 | 0.0015 | 0.0005 | 0.0146 | 0.0005 | 0.0008 | 0.0006 | 0.0012 | 0.0008 | 0.0057 | 0.0004 | 0.0019 | 0.0232 | 0.0029 | 0.0005 |
Nuclear Periphery | 0.0063 | 0.0003 | 0.0009 | 0.0019 | 0.0116 | 0.002 | 0.0104 | 0.0006 | 0.0008 | 0.0006 | 0.0022 | 0.0005 | 0.0137 | 0.0002 | 0.0037 | 0.0025 | 0.0091 | 0.0014 |
Nucleolus | 0.0004 | 0 | 0 | 0.0002 | 0.0002 | 0 | 0.0006 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.0009 | 0 |
Nucleus | 0.0054 | 0.0016 | 0.0026 | 0.002 | 0.0043 | 0.0048 | 0.013 | 0.0019 | 0.0013 | 0.0017 | 0.0025 | 0.002 | 0.0064 | 0.0011 | 0.0052 | 0.0023 | 0.025 | 0.0029 |
Peroxisomes | 0.0064 | 0 | 0.0014 | 0.0197 | 0.0008 | 0 | 0.0022 | 0.0001 | 0.0003 | 0 | 0 | 0.0003 | 0.0005 | 0 | 0.0015 | 0.0007 | 0.0059 | 0.0001 |
Punctate Nuclear | 0.0014 | 0 | 0.0007 | 0.0003 | 0.0021 | 0.0001 | 0.0072 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0.0002 | 0.0021 | 0 | 0.0011 | 0.0132 | 0.0024 | 0.0005 |
Vacuole | 0.0028 | 0.0001 | 0.0019 | 0.0037 | 0.01 | 0.0008 | 0.0013 | 0.0001 | 0.0019 | 0.0003 | 0.0047 | 0.0005 | 0.0021 | 0.0001 | 0.0018 | 0.001 | 0.0039 | 0.0001 |
Vacuole Periphery | 0.001 | 0 | 0.0007 | 0.0012 | 0.0029 | 0.0002 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0017 | 0 | 0.0004 | 0.0005 | 0.0012 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 84.9341 | 59.4203 | 68.5882 | 65.2248 | 60.327 | 51.6704 | 59.0784 | 67.1324 | 66.087 | 73.6619 |
Translational Efficiency | 1.6732 | 2.038 | 1.4894 | 1.8556 | 1.9734 | 2.7495 | 1.5823 | 1.3696 | 1.4957 | 1.5227 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2497 | 1614 | 2613 | 84 | 2229 | 2289 | 810 | 1775 | 4726 | 3903 | 3423 | 1859 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1744.15 | 1881.97 | 2060.89 | 1773.95 | 1660.71 | 2004.61 | 1983.23 | 1694.04 | 1704.80 | 1953.89 | 2042.51 | 1697.65 |
Standard Deviation | 287.73 | 399.70 | 336.91 | 466.86 | 284.34 | 336.02 | 371.25 | 439.37 | 289.15 | 368.69 | 346.91 | 440.96 |
Intensity Change Log 2 | — | 0.109720 | 0.240743 | 0.024441 | — | 0.271521 | 0.256052 | 0.028668 | — | 0.190906 | 0.248230 | 0.026504 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000054 | 0.000108 | 0.000070 | 0.000860 | 0.000064 | 0.000089 | 0.000234 | 0.000615 | 0.000059 | 0.000097 | 0.000109 | 0.000626 |
Bud Neck | 0.000243 | 0.000327 | 0.000625 | 0.003222 | 0.000283 | 0.000261 | 0.001789 | 0.001044 | 0.000262 | 0.000288 | 0.000900 | 0.001142 |
Bud Site | 0.001255 | 0.001285 | 0.001559 | 0.034568 | 0.001361 | 0.001879 | 0.004103 | 0.008921 | 0.001305 | 0.001634 | 0.002161 | 0.010080 |
Cell Periphery | 0.000034 | 0.000106 | 0.000075 | 0.000279 | 0.000037 | 0.000044 | 0.000188 | 0.000203 | 0.000035 | 0.000069 | 0.000102 | 0.000206 |
Cytoplasm | 0.912282* | 0.873294* | 0.916788* | 0.710462* | 0.886747* | 0.902937* | 0.820834* | 0.854992* | 0.900238* | 0.890679* | 0.894082* | 0.848461* |
Cytoplasmic Foci | 0.007834 | 0.005325 | 0.005513 | 0.033787 | 0.005892 | 0.007369 | 0.011827 | 0.009880 | 0.006918 | 0.006524 | 0.007007 | 0.010960 |
Eisosomes | 0.000001 | 0.000001 | 0.000001 | 0.000010 | 0.000001 | 0.000001 | 0.000002 | 0.000005 | 0.000001 | 0.000001 | 0.000001 | 0.000005 |
Endoplasmic Reticulum | 0.000202 | 0.000130 | 0.000081 | 0.000255 | 0.000110 | 0.000070 | 0.000293 | 0.000448 | 0.000159 | 0.000095 | 0.000131 | 0.000439 |
Endosome | 0.000250 | 0.000306 | 0.000407 | 0.015195 | 0.000140 | 0.000376 | 0.001378 | 0.001912 | 0.000198 | 0.000347 | 0.000636 | 0.002512 |
Golgi | 0.000403 | 0.000873 | 0.000819 | 0.026895 | 0.000315 | 0.000791 | 0.001780 | 0.004849 | 0.000361 | 0.000825 | 0.001046 | 0.005845 |
Lipid Particles | 0.000285 | 0.000257 | 0.000754 | 0.001396 | 0.000146 | 0.000288 | 0.001494 | 0.001013 | 0.000220 | 0.000275 | 0.000929 | 0.001031 |
Mitochondria | 0.000096 | 0.000062 | 0.000095 | 0.001583 | 0.000075 | 0.000072 | 0.000110 | 0.000437 | 0.000086 | 0.000068 | 0.000099 | 0.000489 |
Mitotic Spindle | 0.000993 | 0.001312 | 0.002099 | 0.005239 | 0.001169 | 0.002121 | 0.009911 | 0.008903 | 0.001076 | 0.001786 | 0.003948 | 0.008738 |
None | 0.005377 | 0.014546 | 0.002603 | 0.003009 | 0.011589 | 0.013467 | 0.006248 | 0.006905 | 0.008306 | 0.013913 | 0.003465 | 0.006729 |
Nuclear Periphery | 0.000074 | 0.000045 | 0.000096 | 0.000239 | 0.000048 | 0.000029 | 0.000278 | 0.000100 | 0.000062 | 0.000036 | 0.000139 | 0.000107 |
Nuclear Periphery Foci | 0.001595 | 0.002323 | 0.001484 | 0.004148 | 0.001716 | 0.001353 | 0.004837 | 0.001234 | 0.001652 | 0.001754 | 0.002277 | 0.001366 |
Nucleolus | 0.000099 | 0.000129 | 0.000109 | 0.000101 | 0.000085 | 0.000082 | 0.000135 | 0.000124 | 0.000093 | 0.000101 | 0.000115 | 0.000123 |
Nucleus | 0.061271 | 0.094556 | 0.051226 | 0.060066 | 0.086346 | 0.064393 | 0.108647 | 0.075708 | 0.073098 | 0.076866 | 0.064814 | 0.075001 |
Peroxisomes | 0.000022 | 0.000024 | 0.000097 | 0.001315 | 0.000020 | 0.000136 | 0.000048 | 0.000202 | 0.000021 | 0.000089 | 0.000085 | 0.000252 |
Vacuole | 0.006906 | 0.004599 | 0.015130 | 0.082766 | 0.003613 | 0.004022 | 0.025463 | 0.021310 | 0.005353 | 0.004260 | 0.017575 | 0.024087 |
Vacuole Periphery | 0.000723 | 0.000392 | 0.000368 | 0.014607 | 0.000244 | 0.000220 | 0.000402 | 0.001195 | 0.000497 | 0.000291 | 0.000376 | 0.001801 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.72 | -2.74 | -4.24 | -3.61 | -3.93 | -2.06 | -9.25 | -3.21 | -3.00 | -0.35 | -4.64 | -8.35 | -3.41 | -3.03 | -2.48 |
Bud Neck | -3.40 | -9.89 | -6.74 | -6.40 | -5.24 | 0.19 | -12.42 | -10.73 | -11.61 | 7.12 | -1.72 | -15.10 | -12.72 | -12.19 | -0.40 |
Bud Site | -0.65 | -2.21 | -3.52 | -3.48 | -3.37 | -1.21 | -8.28 | -8.92 | -8.20 | -1.49 | -1.42 | -6.50 | -10.03 | -9.46 | -7.59 |
Cell Periphery | -6.81 | -10.74 | -5.68 | -4.34 | -4.69 | -2.43 | -11.99 | -8.41 | -7.85 | 5.51 | -7.26 | -15.60 | -9.43 | -6.55 | -1.65 |
Cytoplasm | 11.68 | 6.28 | 11.82 | 10.37 | 11.16 | -4.92 | 20.11 | 21.11 | 23.90 | -5.13 | 4.95 | 14.54 | 26.22 | 23.31 | 15.40 |
Cytoplasmic Foci | 2.49 | 1.90 | -5.21 | -5.50 | -5.41 | -1.92 | -8.81 | -6.95 | -4.02 | 4.88 | 0.26 | -4.78 | -7.04 | -6.68 | -3.37 |
Eisosomes | -1.57 | -1.91 | -8.66 | -8.54 | -8.53 | -2.69 | -12.24 | -6.11 | -5.80 | -1.23 | -2.65 | -10.02 | -6.81 | -6.55 | -5.33 |
Endoplasmic Reticulum | 1.66 | 3.01 | -1.39 | -2.23 | -2.72 | 2.54 | -2.21 | -1.07 | -1.43 | 0.24 | 2.86 | 0.82 | -0.72 | -1.29 | -0.91 |
Endosome | -0.62 | -4.77 | -1.48 | -1.46 | -1.35 | -1.73 | -6.35 | -3.34 | -2.17 | 1.67 | -1.65 | -8.50 | -3.42 | -2.74 | -1.13 |
Golgi | -2.28 | -5.42 | -1.00 | -0.89 | -0.88 | -2.48 | -2.69 | -2.34 | -1.64 | -0.85 | -3.28 | -5.70 | -2.46 | -1.78 | -1.56 |
Lipid Particles | -0.16 | -7.31 | -6.14 | -6.13 | -4.19 | -1.16 | -10.54 | -6.83 | -4.95 | 4.82 | -0.90 | -12.45 | -7.03 | -6.13 | 0.71 |
Mitochondria | 1.60 | -0.17 | -0.94 | -1.08 | -0.92 | 0.17 | -2.84 | -3.00 | -3.08 | -2.16 | 1.16 | -1.17 | -2.99 | -3.32 | -2.63 |
Mitotic Spindle | -0.53 | -3.21 | -2.17 | -2.09 | -1.75 | -1.25 | -3.62 | -6.25 | -5.27 | -0.86 | -1.42 | -4.76 | -6.69 | -5.97 | -4.18 |
None | -7.92 | 9.34 | 2.88 | 8.46 | -0.63 | -1.66 | 7.69 | 6.80 | 8.81 | -1.84 | -7.47 | 13.22 | 4.44 | 10.78 | -7.71 |
Nuclear Periphery | 1.52 | -4.41 | -6.53 | -6.93 | -5.66 | 4.46 | -13.48 | -14.29 | -15.87 | 5.77 | 2.75 | -12.51 | -13.01 | -16.40 | -3.15 |
Nuclear Periphery Foci | -3.74 | -1.85 | -5.02 | -3.82 | -4.24 | 1.81 | -6.38 | 0.80 | -1.48 | 6.84 | -0.90 | -5.15 | -0.45 | 0.47 | 4.93 |
Nucleolus | -0.89 | -2.14 | -1.40 | -0.05 | -0.21 | 0.00 | -5.04 | -2.36 | -3.68 | 3.38 | -0.56 | -4.30 | -2.72 | -1.97 | 0.44 |
Nucleus | -12.10 | 0.14 | -1.52 | 1.25 | -1.54 | 8.75 | -10.73 | -6.67 | -13.45 | 5.64 | -2.90 | -3.27 | -11.30 | -9.28 | -8.41 |
Peroxisomes | -0.37 | -1.13 | -1.93 | -1.91 | -1.53 | -1.87 | -7.74 | -4.25 | 0.09 | -1.76 | -1.89 | -1.49 | -4.62 | -1.14 | -1.06 |
Vacuole | 1.52 | -13.25 | -8.98 | -9.02 | -8.34 | -1.28 | -14.98 | -21.58 | -21.22 | 0.59 | 1.09 | -20.30 | -21.86 | -21.97 | -11.41 |
Vacuole Periphery | 0.88 | 0.98 | -0.72 | -0.88 | -0.89 | 0.17 | -0.30 | -1.18 | -1.51 | -1.17 | 0.98 | 0.73 | -0.35 | -1.47 | -1.24 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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