Standard name
Human Ortholog
Description S-adenosylmethionine-dependent lysine methyltransferase; involved in the trimethylation of eEF1A (Tef1p/Tef2p) at lysine 79; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in budding yeast; expresses a circular RNA; originally misclassified as a N-6-adenine specific DNA methyltransferase based on sequence similarity; both Efm5p and human ortholog N6AMT2 can methylate eEF1a from either species in vitro
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.99 0.97 0.98 0.94 0.88 0.84 0.81 0.85 0.85 0.78 – 0.98 0.99 0.98 0.97 0.97 0.98 0.93 0.96 0.84 0.86 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.05 0.05 0.06 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.08 0 0 0.1 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0.07 0.05 0.07 0.08 – 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 4 1 5 2 4 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 1 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 2 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 2 4 0 3 5 10 7 10 10 14 4 9 – 1 1 2 1 1 0 0 0 0 0 0
Cytoplasm 198 390 231 204 262 311 324 369 241 256 243 238 – 124 67 257 186 203 194 372 221 43 77 116
Endoplasmic Reticulum 0 0 1 0 3 9 23 23 15 18 8 13 – 2 0 8 8 6 0 2 1 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 1 1 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0
Mitochondria 0 1 0 0 0 1 1 3 24 6 12 29 – 0 0 2 1 1 0 2 0 0 1 0
Nucleus 2 1 0 5 4 5 9 18 3 5 5 7 – 0 1 5 1 1 1 8 2 1 4 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 1 2 2 2 2 8 14 32 20 15 20 23 – 3 0 1 8 9 0 9 1 2 3 3
Unique Cell Count 199 391 233 210 266 332 368 438 297 300 285 304 127 68 261 191 209 198 403 230 52 90 131
Labelled Cell Count 203 399 235 214 276 345 380 460 315 319 294 323 130 69 275 206 223 198 403 230 52 90 131


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 27.1 14.4 21.6 20.1 19.6 15.8 14.8 14.3 12.6 13.6 13.1 13.3 – 20.3 21.4 17.1 20.1 21.4 17.0 20.4 21.7
Std Deviation (1e-4) 4.3 3.7 3.7 3.7 3.3 3.2 3.0 2.7 3.1 2.9 3.2 3.1 – 4.9 4.3 4.3 5.0 5.5 4.3 6.3 6.9
Intensity Change (Log2) – – – -0.1 -0.14 -0.45 -0.54 -0.59 -0.78 -0.67 -0.72 -0.71 – -0.09 -0.02 -0.34 -0.1 -0.02 -0.35 -0.08 0.01

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000102030WT3HU120HU1600102030WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30102030WT1AF100AF140AF1800102030
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 3.3 0 0 – 0 0 0 0 0
Cytoplasm -1.6 -0.7 -3.2 -5.0 -6.0 -6.6 -5.6 -5.6 -7.2 – -1.2 -0.4 -0.7 -1.4 -1.6
Endoplasmic Reticulum 0 0 0 3.6 3.2 3.1 3.4 0 2.8 – 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.4 0 3.2 4.8 – 0 0 0 0 0
Nucleus 0 0 0 0 3.1 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole 0 0 0 2.2 3.6 3.4 2.7 3.5 3.7 – 0 0 0 0 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 16.6467 20.2234 19.0283 16.8343 17.1273 18.6705 15.0709 19.2399 17.0171 17.1778 15.6203 17.1296 13.4839 18.2349 15.4293 15.075 13.4337 16.1586
Actin 0.0162 0.0002 0.0007 0.0142 0.0131 0.0003 0.0111 0.0014 0.0026 0.0003 0.0002 0.0047 0.0083 0.0002 0.0024 0.0012 0.0074 0.0055
Bud 0.0005 0.0001 0.0001 0.0002 0.0004 0.0001 0.0004 0.0001 0.0003 0.0001 0.0001 0.0002 0.0004 0.0001 0.0002 0.0001 0.0008 0.0001
Bud Neck 0.0009 0.0001 0.0003 0.001 0.0008 0.0008 0.0008 0.0001 0.0004 0.0003 0.0004 0.0009 0.0006 0.0001 0.0004 0.0003 0.0007 0.0007
Bud Periphery 0.0008 0 0 0.0001 0.0004 0 0.0002 0 0.0003 0 0 0.0005 0.0004 0 0.0001 0 0.0008 0
Bud Site 0.0014 0.0001 0.0001 0.0008 0.0003 0 0.0004 0.0002 0.0004 0.0001 0.0001 0.0018 0.0005 0.0001 0.0023 0.0001 0.0055 0
Cell Periphery 0.0002 0 0 0 0.0001 0 0.0001 0.0001 0.0001 0 0 0.0011 0.0001 0 0.0001 0 0.0004 0
Cytoplasm 0.8996 0.9959 0.9693 0.8935 0.8656 0.9846 0.8687 0.985 0.9635 0.9908 0.963 0.9729 0.9341 0.9968 0.952 0.9093 0.8006 0.9825
Cytoplasmic Foci 0.0086 0.0005 0.0102 0.0123 0.0125 0.0015 0.0181 0.0022 0.0074 0.0017 0.0091 0.0048 0.0043 0.0004 0.0075 0.0075 0.0428 0.0014
Eisosomes 0.0002 0 0 0 0.0001 0 0.0001 0 0 0 0 0.0003 0.0001 0 0 0.0001 0.0002 0
Endoplasmic Reticulum 0.008 0.0004 0.001 0.002 0.0062 0.003 0.0072 0.0024 0.0027 0.002 0.0027 0.002 0.0056 0.0003 0.0014 0.0013 0.0046 0.0012
Endosome 0.0191 0.0002 0.0078 0.0269 0.0555 0.0011 0.0309 0.0038 0.0108 0.0012 0.0111 0.0044 0.0099 0.0001 0.0115 0.02 0.0309 0.002
Golgi 0.0045 0 0.0006 0.0031 0.0047 0.0001 0.0064 0.0009 0.0022 0.0001 0.0003 0.0009 0.0013 0 0.0021 0.0011 0.0178 0.0006
Lipid Particles 0.0067 0 0.0004 0.0141 0.0049 0 0.0043 0 0.0019 0 0.0008 0.0005 0.0006 0 0.0034 0.0142 0.0283 0.0002
Mitochondria 0.0073 0 0.0005 0.002 0.002 0.0002 0.0016 0.0004 0.0016 0.0001 0.0001 0.0006 0.0016 0 0.001 0.0006 0.0078 0.0002
None 0.0023 0.0003 0.0005 0.0007 0.0015 0.0005 0.0146 0.0005 0.0008 0.0006 0.0012 0.0008 0.0057 0.0004 0.0019 0.0232 0.0029 0.0005
Nuclear Periphery 0.0063 0.0003 0.0009 0.0019 0.0116 0.002 0.0104 0.0006 0.0008 0.0006 0.0022 0.0005 0.0137 0.0002 0.0037 0.0025 0.0091 0.0014
Nucleolus 0.0004 0 0 0.0002 0.0002 0 0.0006 0 0 0 0.0001 0 0.0001 0 0.0001 0.0006 0.0009 0
Nucleus 0.0054 0.0016 0.0026 0.002 0.0043 0.0048 0.013 0.0019 0.0013 0.0017 0.0025 0.002 0.0064 0.0011 0.0052 0.0023 0.025 0.0029
Peroxisomes 0.0064 0 0.0014 0.0197 0.0008 0 0.0022 0.0001 0.0003 0 0 0.0003 0.0005 0 0.0015 0.0007 0.0059 0.0001
Punctate Nuclear 0.0014 0 0.0007 0.0003 0.0021 0.0001 0.0072 0.0001 0.0002 0.0001 0.001 0.0002 0.0021 0 0.0011 0.0132 0.0024 0.0005
Vacuole 0.0028 0.0001 0.0019 0.0037 0.01 0.0008 0.0013 0.0001 0.0019 0.0003 0.0047 0.0005 0.0021 0.0001 0.0018 0.001 0.0039 0.0001
Vacuole Periphery 0.001 0 0.0007 0.0012 0.0029 0.0002 0.0006 0.0001 0.0003 0.0001 0.0004 0.0001 0.0017 0 0.0004 0.0005 0.0012 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 84.9341 59.4203 68.5882 65.2248 60.327 51.6704 59.0784 67.1324 66.087 73.6619
Translational Efficiency 1.6732 2.038 1.4894 1.8556 1.9734 2.7495 1.5823 1.3696 1.4957 1.5227

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2497 1614 2613 84 2229 2289 810 1775 4726 3903 3423 1859

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1744.15 1881.97 2060.89 1773.95 1660.71 2004.61 1983.23 1694.04 1704.80 1953.89 2042.51 1697.65
Standard Deviation 287.73 399.70 336.91 466.86 284.34 336.02 371.25 439.37 289.15 368.69 346.91 440.96
Intensity Change Log 2 — 0.109720 0.240743 0.024441 — 0.271521 0.256052 0.028668 — 0.190906 0.248230 0.026504

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000054 0.000108 0.000070 0.000860 0.000064 0.000089 0.000234 0.000615 0.000059 0.000097 0.000109 0.000626
Bud Neck 0.000243 0.000327 0.000625 0.003222 0.000283 0.000261 0.001789 0.001044 0.000262 0.000288 0.000900 0.001142
Bud Site 0.001255 0.001285 0.001559 0.034568 0.001361 0.001879 0.004103 0.008921 0.001305 0.001634 0.002161 0.010080
Cell Periphery 0.000034 0.000106 0.000075 0.000279 0.000037 0.000044 0.000188 0.000203 0.000035 0.000069 0.000102 0.000206
Cytoplasm 0.912282 0.873294 0.916788 0.710462 0.886747 0.902937 0.820834 0.854992 0.900238 0.890679 0.894082 0.848461
Cytoplasmic Foci 0.007834 0.005325 0.005513 0.033787 0.005892 0.007369 0.011827 0.009880 0.006918 0.006524 0.007007 0.010960
Eisosomes 0.000001 0.000001 0.000001 0.000010 0.000001 0.000001 0.000002 0.000005 0.000001 0.000001 0.000001 0.000005
Endoplasmic Reticulum 0.000202 0.000130 0.000081 0.000255 0.000110 0.000070 0.000293 0.000448 0.000159 0.000095 0.000131 0.000439
Endosome 0.000250 0.000306 0.000407 0.015195 0.000140 0.000376 0.001378 0.001912 0.000198 0.000347 0.000636 0.002512
Golgi 0.000403 0.000873 0.000819 0.026895 0.000315 0.000791 0.001780 0.004849 0.000361 0.000825 0.001046 0.005845
Lipid Particles 0.000285 0.000257 0.000754 0.001396 0.000146 0.000288 0.001494 0.001013 0.000220 0.000275 0.000929 0.001031
Mitochondria 0.000096 0.000062 0.000095 0.001583 0.000075 0.000072 0.000110 0.000437 0.000086 0.000068 0.000099 0.000489
Mitotic Spindle 0.000993 0.001312 0.002099 0.005239 0.001169 0.002121 0.009911 0.008903 0.001076 0.001786 0.003948 0.008738
None 0.005377 0.014546 0.002603 0.003009 0.011589 0.013467 0.006248 0.006905 0.008306 0.013913 0.003465 0.006729
Nuclear Periphery 0.000074 0.000045 0.000096 0.000239 0.000048 0.000029 0.000278 0.000100 0.000062 0.000036 0.000139 0.000107
Nuclear Periphery Foci 0.001595 0.002323 0.001484 0.004148 0.001716 0.001353 0.004837 0.001234 0.001652 0.001754 0.002277 0.001366
Nucleolus 0.000099 0.000129 0.000109 0.000101 0.000085 0.000082 0.000135 0.000124 0.000093 0.000101 0.000115 0.000123
Nucleus 0.061271 0.094556 0.051226 0.060066 0.086346 0.064393 0.108647 0.075708 0.073098 0.076866 0.064814 0.075001
Peroxisomes 0.000022 0.000024 0.000097 0.001315 0.000020 0.000136 0.000048 0.000202 0.000021 0.000089 0.000085 0.000252
Vacuole 0.006906 0.004599 0.015130 0.082766 0.003613 0.004022 0.025463 0.021310 0.005353 0.004260 0.017575 0.024087
Vacuole Periphery 0.000723 0.000392 0.000368 0.014607 0.000244 0.000220 0.000402 0.001195 0.000497 0.000291 0.000376 0.001801

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.72 -2.74 -4.24 -3.61 -3.93 -2.06 -9.25 -3.21 -3.00 -0.35 -4.64 -8.35 -3.41 -3.03 -2.48
Bud Neck -3.40 -9.89 -6.74 -6.40 -5.24 0.19 -12.42 -10.73 -11.61 7.12 -1.72 -15.10 -12.72 -12.19 -0.40
Bud Site -0.65 -2.21 -3.52 -3.48 -3.37 -1.21 -8.28 -8.92 -8.20 -1.49 -1.42 -6.50 -10.03 -9.46 -7.59
Cell Periphery -6.81 -10.74 -5.68 -4.34 -4.69 -2.43 -11.99 -8.41 -7.85 5.51 -7.26 -15.60 -9.43 -6.55 -1.65
Cytoplasm 11.68 6.28 11.82 10.37 11.16 -4.92 20.11 21.11 23.90 -5.13 4.95 14.54 26.22 23.31 15.40
Cytoplasmic Foci 2.49 1.90 -5.21 -5.50 -5.41 -1.92 -8.81 -6.95 -4.02 4.88 0.26 -4.78 -7.04 -6.68 -3.37
Eisosomes -1.57 -1.91 -8.66 -8.54 -8.53 -2.69 -12.24 -6.11 -5.80 -1.23 -2.65 -10.02 -6.81 -6.55 -5.33
Endoplasmic Reticulum 1.66 3.01 -1.39 -2.23 -2.72 2.54 -2.21 -1.07 -1.43 0.24 2.86 0.82 -0.72 -1.29 -0.91
Endosome -0.62 -4.77 -1.48 -1.46 -1.35 -1.73 -6.35 -3.34 -2.17 1.67 -1.65 -8.50 -3.42 -2.74 -1.13
Golgi -2.28 -5.42 -1.00 -0.89 -0.88 -2.48 -2.69 -2.34 -1.64 -0.85 -3.28 -5.70 -2.46 -1.78 -1.56
Lipid Particles -0.16 -7.31 -6.14 -6.13 -4.19 -1.16 -10.54 -6.83 -4.95 4.82 -0.90 -12.45 -7.03 -6.13 0.71
Mitochondria 1.60 -0.17 -0.94 -1.08 -0.92 0.17 -2.84 -3.00 -3.08 -2.16 1.16 -1.17 -2.99 -3.32 -2.63
Mitotic Spindle -0.53 -3.21 -2.17 -2.09 -1.75 -1.25 -3.62 -6.25 -5.27 -0.86 -1.42 -4.76 -6.69 -5.97 -4.18
None -7.92 9.34 2.88 8.46 -0.63 -1.66 7.69 6.80 8.81 -1.84 -7.47 13.22 4.44 10.78 -7.71
Nuclear Periphery 1.52 -4.41 -6.53 -6.93 -5.66 4.46 -13.48 -14.29 -15.87 5.77 2.75 -12.51 -13.01 -16.40 -3.15
Nuclear Periphery Foci -3.74 -1.85 -5.02 -3.82 -4.24 1.81 -6.38 0.80 -1.48 6.84 -0.90 -5.15 -0.45 0.47 4.93
Nucleolus -0.89 -2.14 -1.40 -0.05 -0.21 0.00 -5.04 -2.36 -3.68 3.38 -0.56 -4.30 -2.72 -1.97 0.44
Nucleus -12.10 0.14 -1.52 1.25 -1.54 8.75 -10.73 -6.67 -13.45 5.64 -2.90 -3.27 -11.30 -9.28 -8.41
Peroxisomes -0.37 -1.13 -1.93 -1.91 -1.53 -1.87 -7.74 -4.25 0.09 -1.76 -1.89 -1.49 -4.62 -1.14 -1.06
Vacuole 1.52 -13.25 -8.98 -9.02 -8.34 -1.28 -14.98 -21.58 -21.22 0.59 1.09 -20.30 -21.86 -21.97 -11.41
Vacuole Periphery 0.88 0.98 -0.72 -0.88 -0.89 0.17 -0.30 -1.18 -1.51 -1.17 0.98 0.73 -0.35 -1.47 -1.24
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description S-adenosylmethionine-dependent lysine methyltransferase; involved in the trimethylation of eEF1A (Tef1p/Tef2p) at lysine 79; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in budding yeast; expresses a circular RNA; originally misclassified as a N-6-adenine specific DNA methyltransferase based on sequence similarity; both Efm5p and human ortholog N6AMT2 can methylate eEF1a from either species in vitro
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Efm5

Efm5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Efm5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available