Standard name | |
---|---|
Human Ortholog | |
Description | Ethanolamine-phosphate cytidylyltransferase; catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases; inability of the null mutant to synthesize phosphatidylethanolamine and phosphatidylcholine from ethanolamine is functionally complemented by human PCYT2 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 0 | 12 | 8 | 8 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 3 | 0 | 4 | 3 | 4 | 2 | 3 | 5 | 8 | 8 | 1 | 1 | 2 | 10 | 10 | 15 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 743 | 376 | 352 | 335 | 533 | 429 | 391 | 397 | 387 | 407 | 262 | 349 | 255 | 366 | 387 | 301 | 315 | 309 | 713 | 352 | 339 | 187 | 315 | 244 |
Endoplasmic Reticulum | 2 | 1 | 1 | 1 | 1 | 1 | 5 | 4 | 6 | 0 | 2 | 3 | 0 | 1 | 4 | 7 | 8 | 6 | 0 | 2 | 3 | 0 | 1 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 3 | 1 | 1 | 2 | 1 | 2 | 2 | 2 | 0 |
Golgi | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 4 | 1 | 0 | 1 | 7 | 32 | 66 | 48 | 80 | 94 | 138 | 83 | 0 | 0 | 0 | 3 | 3 | 6 | 4 | 1 | 3 | 0 | 3 | 2 |
Nucleus | 4 | 1 | 1 | 1 | 3 | 14 | 14 | 18 | 10 | 20 | 9 | 20 | 1 | 4 | 3 | 3 | 2 | 3 | 2 | 4 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Vac/Vac Membrane | 5 | 3 | 4 | 14 | 54 | 58 | 68 | 45 | 40 | 93 | 18 | 62 | 3 | 14 | 16 | 25 | 28 | 16 | 1 | 2 | 1 | 0 | 4 | 2 |
Unique Cell Count | 751 | 381 | 358 | 345 | 581 | 503 | 500 | 496 | 490 | 567 | 391 | 485 | 258 | 372 | 398 | 320 | 337 | 332 | 738 | 369 | 356 | 194 | 338 | 257 |
Labelled Cell Count | 759 | 384 | 363 | 352 | 604 | 539 | 551 | 523 | 527 | 632 | 449 | 534 | 261 | 387 | 412 | 352 | 371 | 359 | 738 | 369 | 356 | 194 | 338 | 257 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.1 | 11.0 | 8.6 | 8.3 | 8.4 | 7.1 | 6.5 | 6.9 | 5.9 | 6.1 | 5.4 | 5.9 | 9.1 | 9.2 | 8.9 | 11.1 | 12.2 | 11.7 | 10.2 | 10.3 | 11.6 |
Std Deviation (1e-4) | 1.1 | 1.5 | 1.2 | 1.0 | 1.2 | 1.2 | 1.1 | 1.0 | 1.0 | 0.8 | 1.0 | 0.9 | 1.5 | 1.0 | 1.1 | 2.0 | 2.0 | 2.1 | 1.4 | 1.5 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.03 | -0.29 | -0.4 | -0.32 | -0.54 | -0.51 | -0.68 | -0.55 | 0.08 | 0.09 | 0.04 | 0.37 | 0.5 | 0.44 | 0.24 | 0.26 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 | 0 | 0 | 0 | 2.2 | 2.1 | 3.0 |
Cytoplasm | -1.1 | -4.2 | -6.5 | -8.5 | -8.0 | -8.3 | -10.2 | -11.1 | -10.1 | 0.5 | 0.1 | -1.0 | -2.9 | -3.3 | -3.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.9 | 7.2 | 6.1 | 8.0 | 8.1 | 12.4 | 8.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 2.8 | 2.8 | 3.3 | 0 | 3.2 | 0 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.5 | 5.1 | 5.8 | 6.5 | 4.9 | 4.6 | 7.4 | 2.8 | 6.2 | 0 | 2.3 | 2.5 | 4.3 | 4.5 | 2.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2798 | 3.6176 | 3.3167 | 3.1634 | 2.5646 | 3.1738 | 4.1094 | 5.4237 | 4.9905 | 4.5781 | 4.8187 | 5.0176 | 4.846 | 5.8037 | 5.038 | 4.1924 | 5.2976 | 5.2047 |
Actin | 0.0292 | 0.0007 | 0.0052 | 0.0101 | 0.0551 | 0.0043 | 0.0005 | 0.0013 | 0.002 | 0.0059 | 0.0006 | 0.001 | 0.0376 | 0.0011 | 0.0082 | 0.0061 | 0.0002 | 0.002 |
Bud | 0.0014 | 0.0004 | 0.0008 | 0.0009 | 0.0007 | 0.0006 | 0.0019 | 0.0058 | 0.0027 | 0.0212 | 0.0096 | 0.0031 | 0.0016 | 0.0101 | 0.0129 | 0.0055 | 0.0148 | 0.009 |
Bud Neck | 0.0088 | 0.0005 | 0.0063 | 0.0017 | 0.0013 | 0.0023 | 0.0009 | 0.0006 | 0.0008 | 0.0014 | 0.001 | 0.0069 | 0.0038 | 0.0004 | 0.0054 | 0.0354 | 0.0008 | 0.0023 |
Bud Periphery | 0.0017 | 0.0001 | 0.001 | 0.0007 | 0.0006 | 0.0005 | 0.0016 | 0.0016 | 0.0028 | 0.0044 | 0.0019 | 0.0008 | 0.0013 | 0.0016 | 0.0042 | 0.0013 | 0.0018 | 0.0029 |
Bud Site | 0.0052 | 0.0047 | 0.0032 | 0.0024 | 0.0016 | 0.0003 | 0.0009 | 0.0084 | 0.0011 | 0.0026 | 0.0018 | 0.0003 | 0.013 | 0.0072 | 0.0114 | 0.0021 | 0.0002 | 0.0015 |
Cell Periphery | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0003 |
Cytoplasm | 0.6292 | 0.8932 | 0.8421 | 0.817 | 0.6803 | 0.8398 | 0.8578 | 0.8955 | 0.8702 | 0.8776 | 0.8951 | 0.8507 | 0.7802 | 0.9113 | 0.8678 | 0.8076 | 0.913 | 0.8351 |
Cytoplasmic Foci | 0.0434 | 0.012 | 0.0217 | 0.0185 | 0.0358 | 0.015 | 0.0109 | 0.008 | 0.0067 | 0.0089 | 0.0108 | 0.0116 | 0.0263 | 0.0108 | 0.0117 | 0.0127 | 0.0098 | 0.0135 |
Eisosomes | 0.0003 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0081 | 0.0026 | 0.0044 | 0.0039 | 0.0042 | 0.0045 | 0.003 | 0.002 | 0.0024 | 0.0027 | 0.0031 | 0.0028 | 0.0054 | 0.0023 | 0.0021 | 0.0017 | 0.0012 | 0.002 |
Endosome | 0.0454 | 0.0043 | 0.0086 | 0.012 | 0.029 | 0.0186 | 0.0036 | 0.0032 | 0.0032 | 0.0032 | 0.0047 | 0.005 | 0.0262 | 0.0046 | 0.0036 | 0.0056 | 0.0025 | 0.0125 |
Golgi | 0.0153 | 0.0003 | 0.0016 | 0.0061 | 0.0086 | 0.0024 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0079 | 0.0011 | 0.0014 | 0.0022 | 0.0001 | 0.006 |
Lipid Particles | 0.0113 | 0.0001 | 0.0006 | 0.0008 | 0.0164 | 0.0023 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0089 | 0.0003 | 0.001 | 0.008 | 0 | 0.0011 |
Mitochondria | 0.0103 | 0.0003 | 0.001 | 0.005 | 0.0029 | 0.0041 | 0.0004 | 0.0004 | 0.0005 | 0.0015 | 0.0013 | 0.0003 | 0.0057 | 0.0032 | 0.0015 | 0.0029 | 0.0005 | 0.0099 |
None | 0.0995 | 0.0557 | 0.0621 | 0.0527 | 0.0877 | 0.0354 | 0.0689 | 0.039 | 0.0572 | 0.0128 | 0.0242 | 0.0474 | 0.0213 | 0.017 | 0.0202 | 0.0107 | 0.0109 | 0.0244 |
Nuclear Periphery | 0.0224 | 0.0027 | 0.0042 | 0.0123 | 0.006 | 0.0145 | 0.0035 | 0.0023 | 0.0032 | 0.0028 | 0.0039 | 0.0034 | 0.0072 | 0.0025 | 0.0038 | 0.0042 | 0.0022 | 0.0039 |
Nucleolus | 0.0011 | 0.0001 | 0.0018 | 0.0007 | 0.0003 | 0.0023 | 0.0002 | 0.0002 | 0.0002 | 0.0011 | 0.0006 | 0.0003 | 0.0004 | 0.0009 | 0.0004 | 0.0023 | 0.0005 | 0.0096 |
Nucleus | 0.0299 | 0.0165 | 0.0248 | 0.0291 | 0.0224 | 0.0375 | 0.0386 | 0.0251 | 0.0407 | 0.0455 | 0.0341 | 0.0513 | 0.0314 | 0.0185 | 0.0318 | 0.0612 | 0.0347 | 0.0498 |
Peroxisomes | 0.0154 | 0.0004 | 0.0024 | 0.0093 | 0.0333 | 0.0011 | 0.0014 | 0.0003 | 0.0005 | 0.0014 | 0.0002 | 0.0003 | 0.0117 | 0.0012 | 0.0056 | 0.0181 | 0.0002 | 0.0013 |
Punctate Nuclear | 0.014 | 0.0031 | 0.0058 | 0.0147 | 0.0119 | 0.0095 | 0.0042 | 0.0047 | 0.0044 | 0.0047 | 0.0044 | 0.0133 | 0.0067 | 0.0034 | 0.0052 | 0.0103 | 0.0054 | 0.0101 |
Vacuole | 0.0052 | 0.0019 | 0.0018 | 0.0015 | 0.0012 | 0.0029 | 0.0011 | 0.001 | 0.0009 | 0.0013 | 0.002 | 0.001 | 0.0019 | 0.0016 | 0.0011 | 0.0013 | 0.0009 | 0.0021 |
Vacuole Periphery | 0.002 | 0.0002 | 0.0004 | 0.0005 | 0.0005 | 0.002 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0007 | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 45.4076 | 48.7099 | 48.1932 | 42.3755 | 31.6442 | 29.4986 | 53.6113 | 47.0911 | 50.2469 | 48.3422 |
Translational Efficiency | 0.7748 | 0.8936 | 0.6094 | 0.7655 | 0.984 | 1.0391 | 0.7886 | 0.6652 | 0.6 | 0.7251 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
182 | 1271 | 1960 | 1465 | 1987 | 1594 | 311 | 793 | 2169 | 2865 | 2271 | 2258 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1009.92 | 998.90 | 1107.75 | 974.57 | 934.26 | 991.30 | 1138.86 | 973.16 | 940.61 | 994.67 | 1112.01 | 974.07 |
Standard Deviation | 145.84 | 200.86 | 157.90 | 143.95 | 120.35 | 129.80 | 158.56 | 132.22 | 124.47 | 165.19 | 158.35 | 139.94 |
Intensity Change Log 2 | — | -0.015829 | 0.133391 | -0.051403 | — | 0.085498 | 0.285694 | 0.058853 | — | 0.033752 | 0.208588 | 0.002632 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000316 | 0.000236 | 0.000240 | 0.000396 | 0.000075 | 0.000272 | 0.000388 | 0.006691 | 0.000095 | 0.000256 | 0.000260 | 0.002606 |
Bud Neck | 0.001497 | 0.002030 | 0.002167 | 0.006252 | 0.001085 | 0.002058 | 0.002197 | 0.003347 | 0.001120 | 0.002046 | 0.002171 | 0.005232 |
Bud Site | 0.005783 | 0.008512 | 0.006483 | 0.022201 | 0.001749 | 0.007024 | 0.004354 | 0.042221 | 0.002088 | 0.007684 | 0.006191 | 0.029232 |
Cell Periphery | 0.000268 | 0.000093 | 0.000072 | 0.000177 | 0.000059 | 0.000060 | 0.000167 | 0.000274 | 0.000076 | 0.000075 | 0.000085 | 0.000211 |
Cytoplasm | 0.626649* | 0.795457* | 0.575432* | 0.609031* | 0.755089* | 0.789212* | 0.525983* | 0.548414* | 0.744312* | 0.791983* | 0.568660* | 0.587743* |
Cytoplasmic Foci | 0.140728 | 0.027653 | 0.004575 | 0.008834 | 0.048258 | 0.054240 | 0.017669 | 0.019389 | 0.056017 | 0.042445 | 0.006368 | 0.012541 |
Eisosomes | 0.000064 | 0.000009 | 0.000009 | 0.000022 | 0.000014 | 0.000015 | 0.000039 | 0.000041 | 0.000018 | 0.000012 | 0.000013 | 0.000028 |
Endoplasmic Reticulum | 0.004135 | 0.000633 | 0.001013 | 0.002154 | 0.000966 | 0.000869 | 0.003169 | 0.003489 | 0.001232 | 0.000765 | 0.001308 | 0.002623 |
Endosome | 0.011062 | 0.002410 | 0.000844 | 0.002796 | 0.002063 | 0.003789 | 0.004051 | 0.008311 | 0.002818 | 0.003177 | 0.001283 | 0.004733 |
Golgi | 0.000788 | 0.002576 | 0.000255 | 0.000705 | 0.000277 | 0.002101 | 0.000137 | 0.013928 | 0.000320 | 0.002312 | 0.000239 | 0.005349 |
Lipid Particles | 0.007087 | 0.000857 | 0.000453 | 0.000770 | 0.000699 | 0.000726 | 0.001449 | 0.000928 | 0.001235 | 0.000784 | 0.000589 | 0.000826 |
Mitochondria | 0.000086 | 0.000910 | 0.001017 | 0.001413 | 0.001432 | 0.001109 | 0.000226 | 0.005721 | 0.001319 | 0.001021 | 0.000909 | 0.002926 |
Mitotic Spindle | 0.000325 | 0.010900 | 0.007917 | 0.026421 | 0.001938 | 0.007807 | 0.005846 | 0.028635 | 0.001802 | 0.009179 | 0.007634 | 0.027199 |
None | 0.000957 | 0.011511 | 0.008585 | 0.006159 | 0.003974 | 0.004504 | 0.007779 | 0.010379 | 0.003721 | 0.007612 | 0.008475 | 0.007641 |
Nuclear Periphery | 0.000425 | 0.000165 | 0.000917 | 0.001447 | 0.000258 | 0.000244 | 0.001024 | 0.000832 | 0.000272 | 0.000209 | 0.000932 | 0.001231 |
Nuclear Periphery Foci | 0.008347 | 0.002419 | 0.001590 | 0.003107 | 0.001622 | 0.002013 | 0.006150 | 0.002487 | 0.002186 | 0.002193 | 0.002214 | 0.002890 |
Nucleolus | 0.002864 | 0.000379 | 0.000125 | 0.000353 | 0.000215 | 0.000374 | 0.000417 | 0.000207 | 0.000437 | 0.000376 | 0.000165 | 0.000302 |
Nucleus | 0.062796 | 0.100915 | 0.352493* | 0.184500* | 0.137293* | 0.071992 | 0.269352* | 0.146411* | 0.131042* | 0.084823 | 0.341107* | 0.171123* |
Peroxisomes | 0.002156 | 0.001597 | 0.000119 | 0.000166 | 0.000184 | 0.000864 | 0.000188 | 0.000633 | 0.000349 | 0.001189 | 0.000128 | 0.000330 |
Vacuole | 0.123204 | 0.030149 | 0.035603 | 0.120251 | 0.042376 | 0.050205 | 0.149204 | 0.156145 | 0.049158 | 0.041308 | 0.051160 | 0.132857 |
Vacuole Periphery | 0.000462 | 0.000590 | 0.000092 | 0.002843 | 0.000375 | 0.000520 | 0.000212 | 0.001514 | 0.000382 | 0.000551 | 0.000109 | 0.002377 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.40 | 0.35 | -0.28 | -2.65 | -0.97 | -3.14 | -8.88 | -1.68 | -1.42 | -1.11 | -4.37 | -8.80 | -2.26 | -1.63 | -1.34 |
Bud Neck | -0.87 | -4.52 | -5.98 | -3.53 | -2.58 | -4.22 | -6.15 | -10.56 | -4.95 | -0.68 | -3.55 | -8.55 | -10.48 | -5.43 | -2.67 |
Bud Site | -1.13 | 0.94 | -3.23 | -1.46 | -3.77 | -4.94 | -7.42 | -5.61 | -3.08 | -4.03 | -6.02 | -4.44 | -8.63 | -3.31 | -5.24 |
Cell Periphery | 3.56 | 3.71 | 3.24 | -1.34 | -2.63 | -0.10 | -8.53 | -4.09 | -3.99 | 1.75 | 0.35 | -3.40 | -4.33 | -4.39 | -2.09 |
Cytoplasm | -7.77 | 6.56 | 6.93 | 32.96 | 1.23 | -3.77 | 24.31 | 33.82 | 34.79 | -0.57 | -6.10 | 40.63 | 41.19 | 47.75 | 2.99 |
Cytoplasmic Foci | 9.62 | 11.98 | 11.60 | 11.32 | -5.74 | -2.21 | 14.74 | 17.37 | 17.01 | -0.84 | 4.83 | 24.68 | 22.07 | 20.89 | -7.21 |
Eisosomes | 7.64 | 7.14 | 5.00 | -15.11 | -13.32 | -1.06 | -9.51 | -14.71 | -13.64 | 0.15 | 4.58 | 0.34 | -11.58 | -19.13 | -14.22 |
Endoplasmic Reticulum | 3.45 | 2.61 | 2.20 | -7.48 | -2.34 | 0.64 | -7.72 | -5.11 | -4.89 | 2.25 | 2.95 | -3.88 | -4.75 | -7.77 | -2.06 |
Endosome | 5.05 | 6.13 | 5.76 | 1.67 | -3.67 | -3.15 | -1.85 | -0.98 | 1.81 | 0.45 | -0.51 | 8.00 | 3.87 | 3.22 | -3.76 |
Golgi | -1.63 | 2.36 | 2.15 | 2.65 | -0.64 | -3.19 | 3.59 | -1.89 | -0.27 | -2.00 | -3.93 | 2.43 | -1.65 | 1.94 | -2.07 |
Lipid Particles | 1.69 | 1.72 | 1.61 | -0.73 | -7.60 | -0.15 | -6.80 | -7.86 | -6.05 | 2.05 | 1.22 | 1.05 | -0.08 | -2.34 | -6.99 |
Mitochondria | -2.78 | -7.22 | -7.47 | -0.03 | 0.47 | 0.46 | 1.57 | 0.02 | -0.40 | -1.37 | 0.55 | 0.82 | 0.45 | -0.20 | -0.71 |
Mitotic Spindle | -4.51 | -6.92 | -7.55 | -2.20 | -2.83 | -2.64 | -2.27 | -5.72 | -3.87 | -3.00 | -4.22 | -5.40 | -8.41 | -4.42 | -4.09 |
None | -7.53 | -18.96 | -15.59 | 3.19 | 4.45 | -0.52 | -3.17 | -3.92 | -3.44 | -0.07 | -4.33 | -8.38 | -5.64 | 0.08 | 2.60 |
Nuclear Periphery | 2.18 | -11.43 | -9.55 | -13.69 | -2.13 | 0.38 | -13.53 | -18.23 | -17.16 | -0.38 | 1.62 | -30.64 | -19.10 | -19.24 | -2.94 |
Nuclear Periphery Foci | 4.99 | 5.74 | 5.41 | 1.49 | -0.72 | -0.67 | -5.83 | -2.26 | -0.64 | 4.79 | 0.02 | -0.08 | 0.52 | 0.42 | 0.64 |
Nucleolus | 1.10 | 1.18 | 1.12 | 0.51 | -5.37 | -1.82 | -2.35 | -3.08 | 0.39 | -0.27 | 0.23 | 1.19 | 0.50 | 0.71 | -5.25 |
Nucleus | -6.31 | -41.48 | -21.35 | -20.91 | 22.77 | 17.49 | -13.62 | -10.26 | -19.40 | 7.24 | 12.42 | -45.94 | -19.47 | -30.71 | 25.39 |
Peroxisomes | 0.47 | 1.50 | 1.47 | 2.39 | -1.52 | -4.13 | 1.45 | -0.87 | 3.81 | -1.72 | -2.74 | 2.09 | 1.55 | 3.69 | -2.61 |
Vacuole | 7.13 | 4.69 | -6.73 | -25.85 | -21.25 | -4.59 | -12.85 | -24.93 | -23.08 | -4.43 | -0.21 | -10.75 | -33.72 | -34.01 | -24.26 |
Vacuole Periphery | -0.20 | 4.54 | -0.87 | -0.61 | -2.50 | -0.91 | 3.10 | 0.26 | 0.87 | -1.13 | -1.05 | 4.53 | -1.09 | -0.35 | -2.81 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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