Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.07 0.05 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.72 0.63 0.87 0.91 0.86 0.81 0.71 0.69 0.62 0.61 0.54 0.63 0.96 0.96 0.97 0.9 0.79 0.78 0.39 0.3 0.32 0.46 0.4 0.49
Endoplasmic Reticulum 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.11 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.33 0.51 0 0 0 0.14 0.32 0.3 0.43 0.39 0.63 0.4 0 0 0 0.07 0.07 0.08 0.41 0.43 0.47 0.35 0.35 0.28
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0 0.07 0.07 0.07 0.07 0.06 0.07 0 0.07 0 0 0 0 0.05 0.05 0 0 0 0 0.06 0.06
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 3 1 0 0 0 1 0 0 0 0 3 0 0 0 1 0 3 3 1 6 11 13
Bud 3 3 0 5 4 6 10 11 8 11 8 17 3 4 3 0 3 2 0 0 0 0 6 7
Bud Neck 0 0 3 10 21 14 12 17 9 15 2 16 11 10 9 1 0 0 3 0 0 2 6 15
Bud Site 0 0 0 0 0 0 2 1 3 3 2 5 0 1 0 0 0 2 – – – – – –
Cell Periphery 0 0 2 2 1 0 0 0 0 3 0 3 6 1 5 2 6 4 1 0 0 2 5 8
Cytoplasm 142 45 39 187 320 285 335 239 222 230 109 249 394 475 719 102 170 144 91 30 31 97 208 266
Endoplasmic Reticulum 3 6 1 0 0 0 0 0 0 0 0 0 5 1 1 5 12 20 4 1 0 1 3 5
Endosome 0 0 0 0 3 2 2 0 2 2 0 1 1 2 11 2 10 8 6 2 3 7 20 8
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2 0 0 1 1 2
Mitochondria 65 36 2 7 13 49 151 103 155 148 128 159 2 3 2 8 16 14 95 43 46 73 183 154
Nucleus 0 1 1 0 2 0 1 3 1 3 5 1 0 2 1 2 0 0 0 0 0 0 1 0
Nuclear Periphery 0 2 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 1 0 0 6 0 2 2 0 0 0 0 2 1
SpindlePole 5 0 3 8 27 26 31 23 22 28 3 28 1 5 8 2 10 10 7 3 3 5 30 30
Vac/Vac Membrane 0 1 0 1 6 4 0 1 2 0 2 10 1 3 9 4 15 6 6 7 2 6 20 12
Unique Cell Count 196 71 45 205 370 350 469 344 358 379 202 398 411 493 744 113 216 185 231 101 99 214 521 546
Labelled Cell Count 219 94 54 222 397 386 545 399 425 443 259 490 427 507 775 129 246 213 231 101 99 214 521 546


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 3.9 4.2 4.3 4.6 3.6 3.3 3.7 3.2 3.3 3.0 3.3 4.6 4.5 4.6 5.1 5.4 5.2 4.3 4.6 4.8
Std Deviation (1e-4) 0.6 0.9 0.7 1.4 1.8 1.5 1.4 0.9 1.4 0.9 1.1 0.9 0.7 0.8 1.0 1.4 1.5 1.9 1.3 1.2 1.3
Intensity Change (Log2) – – – 0.03 0.15 -0.19 -0.34 -0.17 -0.38 -0.33 -0.5 -0.32 0.15 0.12 0.15 0.28 0.38 0.33 0.04 0.15 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0722 -0.4163 -0.6703 -1.0785 -0.7497 -0.6898 -0.0029 0.0448 0.2225 0.2213 -0.0005 0.2344 -0.6355 -0.9396 -0.9742 -0.9425 -0.7083 -0.9575
Actin 0.0227 0.0023 0.0071 0.0025 0.0068 0.0084 0.0382 0.0014 0.0227 0.0195 0.022 0.0158 0.0332 0.0026 0.0108 0.0075 0.0879 0.0044
Bud 0.001 0.0024 0.0034 0.0023 0.0059 0.0007 0.0022 0.0034 0.0034 0.0016 0.0017 0.0004 0.0014 0.0029 0.0015 0.002 0.0042 0.0008
Bud Neck 0.0034 0.001 0.0047 0.0026 0.0086 0.0085 0.0141 0.001 0.008 0.0105 0.0016 0.0051 0.0091 0.0012 0.0037 0.0138 0.0237 0.0049
Bud Periphery 0.0019 0.0018 0.0062 0.0025 0.0221 0.0009 0.0059 0.0078 0.0055 0.0041 0.0044 0.0008 0.0042 0.0047 0.0062 0.0037 0.0097 0.0018
Bud Site 0.0052 0.0224 0.0222 0.0181 0.0045 0.0006 0.0088 0.0155 0.0141 0.006 0.0081 0.0007 0.013 0.0393 0.0146 0.001 0.0117 0.0006
Cell Periphery 0.0006 0.0012 0.0012 0.0004 0.001 0.0003 0.0021 0.0008 0.0011 0.0006 0.0004 0.0002 0.0036 0.001 0.0022 0.0003 0.0015 0.0004
Cytoplasm 0.0931 0.1566 0.1058 0.0822 0.1077 0.1389 0.1242 0.1551 0.137 0.104 0.1173 0.2082 0.1247 0.2643 0.1825 0.1723 0.0862 0.2087
Cytoplasmic Foci 0.1572 0.1047 0.1272 0.1121 0.1564 0.1434 0.1098 0.1309 0.0821 0.1046 0.1526 0.1228 0.1426 0.1204 0.1101 0.1238 0.1559 0.1575
Eisosomes 0.0006 0.0007 0.0004 0.0005 0.0006 0.0003 0.0008 0.0003 0.0003 0.0003 0.0003 0.0002 0.0014 0.001 0.001 0.0003 0.0013 0.0002
Endoplasmic Reticulum 0.0037 0.006 0.0037 0.0018 0.0028 0.0037 0.0056 0.0028 0.0024 0.003 0.0035 0.003 0.0138 0.0062 0.0058 0.0036 0.0039 0.0037
Endosome 0.0994 0.0674 0.0877 0.0604 0.0564 0.0637 0.0621 0.0629 0.0492 0.0696 0.0783 0.061 0.0999 0.101 0.0782 0.1365 0.163 0.0991
Golgi 0.0116 0.0129 0.0133 0.0038 0.0144 0.0082 0.0107 0.0062 0.0066 0.0159 0.0161 0.0083 0.015 0.0181 0.018 0.0051 0.0181 0.0135
Lipid Particles 0.0478 0.008 0.0151 0.005 0.0544 0.0209 0.0262 0.0153 0.0086 0.0162 0.0362 0.0094 0.0402 0.0064 0.0154 0.0113 0.0543 0.0139
Mitochondria 0.0409 0.0397 0.0498 0.0055 0.0597 0.0321 0.0164 0.0363 0.031 0.0572 0.0568 0.037 0.0392 0.0275 0.0591 0.031 0.053 0.0676
None 0.464 0.5389 0.508 0.6691 0.4011 0.5089 0.5082 0.5252 0.5842 0.5017 0.4486 0.4885 0.3794 0.3796 0.4536 0.4349 0.1952 0.3801
Nuclear Periphery 0.0021 0.0009 0.0041 0.0024 0.001 0.0044 0.0108 0.0014 0.001 0.0131 0.0027 0.0043 0.0138 0.002 0.0069 0.007 0.0048 0.0045
Nucleolus 0.0026 0.0013 0.0014 0.0023 0.0021 0.0013 0.0023 0.0027 0.0016 0.0022 0.0022 0.0006 0.0019 0.001 0.0013 0.0011 0.0048 0.0011
Nucleus 0.0021 0.0027 0.0028 0.0031 0.0023 0.0034 0.0043 0.0033 0.0025 0.0038 0.0022 0.0017 0.013 0.0017 0.0027 0.003 0.0032 0.0075
Peroxisomes 0.023 0.0174 0.0237 0.0083 0.045 0.0336 0.02 0.0111 0.0186 0.0462 0.0268 0.0204 0.0246 0.0033 0.0131 0.0175 0.0812 0.0122
Punctate Nuclear 0.0045 0.0037 0.0051 0.0081 0.0419 0.0109 0.0164 0.0034 0.0032 0.0102 0.0102 0.0045 0.0107 0.0023 0.0026 0.006 0.0204 0.0076
Vacuole 0.0106 0.0068 0.0052 0.0056 0.004 0.005 0.0085 0.0111 0.0151 0.0075 0.0058 0.0054 0.0127 0.0112 0.0083 0.015 0.0115 0.0071
Vacuole Periphery 0.002 0.0011 0.0018 0.0015 0.0011 0.0018 0.0022 0.002 0.0019 0.0025 0.0021 0.0016 0.0027 0.0023 0.0025 0.0035 0.0043 0.0028

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.0614 5.9962 2.2247 8.0349 7.2473 3.352 6.0628 7.5019 7.2979 8.8558
Translational Efficiency 0.525 0.7655 1.4589 0.5195 0.6268 1.7592 0.5957 0.4073 0.6397 0.6924

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1986 1553 2083 434 2011 2110 1750 1714 3997 3663 3833 2148

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 693.95 725.02 723.24 756.78 652.00 712.46 817.49 770.63 672.84 717.79 766.27 767.83
Standard Deviation 76.56 84.60 83.50 81.78 76.05 85.36 92.73 79.44 79.13 85.27 99.59 80.12
Intensity Change Log 2 — 0.063189 0.059643 0.125042 — 0.127937 0.326329 0.241166 — 0.094917 0.194989 0.182463

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000167 0.001163 0.000561 0.000258 0.000197 0.000478 0.000109 0.000112 0.000182 0.000769 0.000355 0.000142
Bud Neck 0.026496 0.048748 0.043913 0.004822 0.029709 0.061505 0.003007 0.004993 0.028113 0.056096 0.025237 0.004958
Bud Site 0.006172 0.031567 0.017524 0.009239 0.006123 0.017752 0.005197 0.006687 0.006147 0.023609 0.011896 0.007202
Cell Periphery 0.000103 0.000194 0.000116 0.000138 0.000131 0.000181 0.000116 0.000119 0.000117 0.000186 0.000116 0.000123
Cytoplasm 0.360688* 0.187346* 0.404699* 0.491900* 0.374647* 0.215842* 0.480803* 0.542867* 0.367712* 0.203760* 0.439445* 0.532569*
Cytoplasmic Foci 0.326667* 0.345825* 0.059751 0.062696 0.288505* 0.316174* 0.029555 0.073015 0.307467* 0.328746* 0.045965 0.070930
Eisosomes 0.000148 0.000225 0.000055 0.000058 0.000176 0.000178 0.000041 0.000051 0.000162 0.000198 0.000049 0.000052
Endoplasmic Reticulum 0.001518 0.000984 0.002513 0.002752 0.001149 0.001088 0.001774 0.001025 0.001332 0.001044 0.002176 0.001374
Endosome 0.020659 0.053827 0.005270 0.005718 0.016995 0.052463 0.002800 0.009476 0.018815 0.053041 0.004142 0.008717
Golgi 0.007078 0.035619 0.000756 0.000717 0.005609 0.017276 0.000090 0.000442 0.006339 0.025053 0.000452 0.000497
Lipid Particles 0.013004 0.012438 0.002730 0.002384 0.015741 0.014794 0.001697 0.002490 0.014381 0.013795 0.002259 0.002469
Mitochondria 0.021674 0.081395 0.007558 0.000464 0.019587 0.068914 0.000443 0.000885 0.020624 0.074206 0.004309 0.000800
Mitotic Spindle 0.004135 0.009620 0.014142 0.003559 0.003136 0.009349 0.002012 0.003980 0.003632 0.009464 0.008604 0.003895
None 0.001881 0.001642 0.002157 0.002530 0.001899 0.001763 0.005770 0.003440 0.001890 0.001712 0.003807 0.003256
Nuclear Periphery 0.000643 0.000695 0.002096 0.001206 0.000673 0.000761 0.001548 0.001184 0.000658 0.000733 0.001846 0.001188
Nuclear Periphery Foci 0.000304 0.000375 0.000620 0.002382 0.000296 0.000436 0.000916 0.000637 0.000300 0.000410 0.000755 0.000990
Nucleolus 0.001540 0.002123 0.001564 0.000727 0.001434 0.003192 0.000586 0.001146 0.001486 0.002739 0.001118 0.001061
Nucleus 0.130344* 0.051968 0.357937* 0.305691* 0.167544* 0.088039 0.412453* 0.270288* 0.149060* 0.072746 0.382827* 0.277442*
Peroxisomes 0.014209 0.070789 0.007731 0.001142 0.013734 0.038354 0.000397 0.001750 0.013970 0.052105 0.004382 0.001627
Vacuole 0.059829 0.058673 0.067513 0.101247 0.050974 0.085474 0.050513 0.074543 0.055374 0.074111 0.059751 0.079939
Vacuole Periphery 0.002741 0.004783 0.000793 0.000370 0.001743 0.005986 0.000174 0.000868 0.002238 0.005476 0.000510 0.000768

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -10.92 -8.74 -2.18 7.92 3.90 -4.84 2.51 2.73 6.17 0.79 -11.43 -7.18 0.92 11.46 7.18
Bud Neck -7.14 -6.23 9.58 14.33 14.49 -10.15 14.56 13.28 21.37 -3.48 -12.59 1.75 17.60 26.39 15.11
Bud Site -13.19 -7.44 -0.83 7.58 3.07 -8.53 1.14 -0.46 7.28 -1.39 -15.41 -5.39 -0.83 12.01 3.47
Cell Periphery -6.88 -1.34 -2.76 3.45 -1.87 -2.09 1.17 1.14 3.24 0.02 -4.90 0.46 -0.20 4.94 -0.77
Cytoplasm 21.07 -3.33 -6.38 -18.86 -4.41 20.09 -7.65 -16.92 -37.01 -8.69 28.76 -7.48 -19.61 -43.66 -13.04
Cytoplasmic Foci -2.49 44.89 40.47 41.45 0.48 -3.71 48.51 37.01 41.07 -13.83 -3.94 64.82 53.88 56.97 -8.49
Eisosomes -6.24 12.53 9.67 14.08 -1.82 -0.08 15.52 14.95 20.14 -1.76 -4.60 19.84 18.95 24.49 -1.13
Endoplasmic Reticulum 2.03 -4.93 -4.93 -7.91 -1.52 0.36 -7.07 -0.10 -0.67 9.51 1.84 -8.11 -1.73 -5.43 8.80
Endosome -12.92 13.88 11.36 19.37 -0.10 -15.97 14.57 6.02 19.73 -7.21 -20.36 19.77 10.35 26.45 -5.75
Golgi -13.27 10.22 9.59 16.88 0.53 -10.45 11.36 10.54 16.86 -3.93 -16.58 14.97 14.71 23.26 -0.14
Lipid Particles 0.48 12.94 12.69 10.22 0.72 0.77 15.44 14.65 13.94 -3.25 0.68 20.04 19.61 17.37 -0.78
Mitochondria -13.69 7.99 12.27 20.26 12.66 -13.47 10.79 10.54 21.36 -2.81 -19.15 12.96 15.97 29.37 10.71
Mitotic Spindle -3.47 -5.77 0.29 3.35 5.55 -4.17 0.31 -1.17 2.94 -1.49 -5.34 -5.13 -0.84 4.08 4.02
None 0.54 -1.13 -1.07 -1.30 -0.64 0.47 -5.18 -2.80 -3.00 2.49 0.68 -5.17 -3.13 -3.35 0.76
Nuclear Periphery -0.14 -13.83 -7.52 -2.41 6.15 -0.69 -11.96 -5.74 -3.77 5.46 -0.48 -18.25 -7.91 -3.67 8.24
Nuclear Periphery Foci -0.88 -4.98 -5.10 -4.95 -4.30 -1.15 -7.50 -4.57 -2.53 4.18 -1.46 -8.85 -7.03 -5.81 -1.76
Nucleolus -1.79 -1.05 3.98 3.99 4.93 -5.26 2.66 1.21 6.11 -1.67 -5.31 1.35 2.31 6.58 0.93
Nucleus 17.15 -32.92 -13.97 -20.47 4.24 14.87 -33.25 -14.73 -27.67 17.71 21.26 -45.98 -22.05 -36.20 16.44
Peroxisomes -17.50 6.12 14.65 22.36 8.91 -13.03 13.98 12.05 22.18 -5.08 -21.63 13.09 18.19 30.55 6.08
Vacuole 0.26 -5.78 -9.16 -9.18 -7.01 -10.15 -6.20 -10.57 -1.40 -4.11 -7.88 -8.63 -13.22 -7.50 -6.81
Vacuole Periphery -4.73 5.73 7.05 16.10 3.65 -11.40 12.61 5.79 14.18 -6.26 -11.03 9.48 7.74 19.34 -2.44
Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
Localization
Cell Percentages cytoplasm (13%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Eat1

Eat1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Eat1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available