ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.96 0.91 0.6 0.86 0.68 0.73 0.59 0.53 0.59 0.55 0.61 0.82 0.93 0.97 0.75 0.87 0.95 0.93 0.9 0.94 0.86 0.88 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.06 0.05 0.31 0.24 0.33 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.05 0.08 0.05 0.05 0.06 0 0.12 0 0 0 0.09 0.06 0.06 0 0.05 0 0.07 0.07 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.14 0.5 0.12 0.28 0.16 0.38 0.23 0.22 0.17 0.16 0.18 0.11 0 0.26 0.17 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Bud 0 1 0 0 0 1 2 2 4 4 1 3 0 1 0 0 0 0 0 0 1 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 1 0 1 2 2 5 5 0 3 0 1 0 2 2 0 0 0 0 0 0 0
Cytoplasm 256 413 199 98 197 95 171 183 145 138 51 57 64 140 228 89 93 60 245 394 206 147 166 202
Endoplasmic Reticulum 1 5 3 0 6 1 5 0 0 0 0 1 0 0 2 1 1 1 1 0 1 1 0 1
Endosome 0 0 0 4 0 0 0 2 2 0 1 0 0 0 0 0 0 1 0 1 0 1 0 1
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0
Mitochondria 0 8 0 1 1 7 14 17 84 57 30 27 2 0 0 1 4 0 0 5 2 0 1 2
Nucleus 5 8 2 1 6 7 18 16 13 14 4 11 3 2 8 11 6 4 7 22 4 11 12 11
Nuclear Periphery 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 2
Nucleolus 0 0 0 0 0 0 0 0 1 0 4 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Vac/Vac Membrane 10 11 31 81 27 39 38 119 62 51 16 15 14 16 4 31 18 4 2 5 1 2 3 2
Unique Cell Count 259 430 218 163 229 139 233 310 274 233 92 93 78 150 234 119 107 63 264 441 221 172 190 236
Labelled Cell Count 272 449 235 186 237 151 250 343 317 270 107 117 83 161 242 135 124 70 264 441 221 172 190 236


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.9 8.6 11.0 10.4 9.8 7.8 7.7 8.1 6.6 7.0 6.4 6.7 9.4 10.1 9.3 10.2 11.9 12.6 10.5 11.0 11.3
Std Deviation (1e-4) 1.8 1.3 1.5 1.4 1.3 1.7 1.1 1.6 1.2 1.4 1.4 2.0 2.1 1.3 1.2 1.6 2.1 2.6 1.6 1.8 2.0
Intensity Change (Log2) – – – -0.08 -0.16 -0.49 -0.51 -0.44 -0.73 -0.64 -0.79 -0.72 -0.22 -0.12 -0.24 -0.11 0.12 0.2 -0.07 -0.0 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -7.3 -1.7 -5.5 -4.9 -8.2 -9.2 -7.8 -7.3 -6.3 -2.2 0.7 2.9 -4.1 -1.2 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.7 3.5 9.0 7.8 8.9 8.3 0 0 0 0 0 0
Nucleus 0 0 0 3.5 2.6 2.5 2.9 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 7.5 -0.8 3.2 0.6 6.1 2.4 2.1 0.7 0.4 0.8 -1.0 -5.0 2.7 0.6 -1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.7202 8.5058 7.6719 7.3942 6.914 7.8268 7.4847 9.481 8.3016 7.7196 7.4753 7.9827 5.2655 7.6754 6.7754 6.441 5.8911 6.5806
Actin 0.0004 0.0011 0.0061 0.0015 0.0028 0.012 0.0221 0.0006 0.0087 0.0262 0.0002 0.002 0.033 0.0009 0.0014 0.0033 0.0224 0.0042
Bud 0.0001 0.0004 0.0005 0.0001 0.0006 0.0005 0.0005 0.0001 0.0002 0.0004 0.0001 0.0001 0.0005 0.0001 0.0002 0.0043 0.0006 0.0001
Bud Neck 0.0003 0.0003 0.001 0.0008 0.0005 0.0061 0.0012 0.0002 0.0005 0.0009 0.0006 0.0013 0.0028 0.0003 0.0021 0.0024 0.0123 0.0015
Bud Periphery 0 0.0001 0.0004 0 0.001 0.0005 0.0005 0 0.0001 0.0002 0 0 0.0006 0 0.0001 0.0019 0.0014 0
Bud Site 0.0002 0.0007 0.0016 0.0003 0.0004 0.0003 0.0085 0.0003 0.0004 0.0003 0.0001 0.0001 0.0026 0.0004 0.0006 0.0036 0.003 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0007 0 0.0001 0 0 0 0.0002 0 0 0.0001 0.0008 0
Cytoplasm 0.9102 0.9642 0.8688 0.7979 0.821 0.8512 0.8241 0.9744 0.9157 0.8528 0.86 0.9065 0.7297 0.9599 0.916 0.8746 0.7245 0.8882
Cytoplasmic Foci 0.0015 0.0018 0.0123 0.0091 0.0021 0.0069 0.0239 0.0019 0.0037 0.0165 0.0025 0.0068 0.0235 0.0064 0.0035 0.0029 0.0132 0.0027
Eisosomes 0 0 0 0 0.0001 0 0.0002 0 0.0001 0.0001 0 0 0.0005 0 0 0 0.0002 0.0001
Endoplasmic Reticulum 0.0054 0.0024 0.0024 0.0011 0.006 0.0042 0.0065 0.0014 0.0034 0.0013 0.0028 0.0025 0.0093 0.001 0.002 0.0017 0.0048 0.002
Endosome 0.0011 0.0013 0.0156 0.0024 0.0234 0.0091 0.0294 0.0007 0.0064 0.0018 0.0265 0.0035 0.0374 0.001 0.0022 0.0008 0.0251 0.0024
Golgi 0 0.0002 0.0091 0.004 0.0059 0.0069 0.0093 0.0001 0.0018 0.0015 0.0004 0.0013 0.0088 0.0001 0.0008 0.0001 0.0068 0.0006
Lipid Particles 0 0 0.0033 0.0368 0.0004 0.0107 0.0076 0 0.0007 0.0002 0 0.0001 0.0141 0 0.0013 0.0001 0.021 0.0005
Mitochondria 0.0001 0.0017 0.0109 0.0019 0.0754 0.0097 0.0046 0.0001 0.0025 0.0009 0.0005 0.0005 0.0064 0.0002 0.0031 0.0003 0.0069 0.0005
None 0.0072 0.004 0.0021 0.0012 0.0023 0.0022 0.0167 0.0018 0.0192 0.0592 0.0021 0.0057 0.014 0.0023 0.0093 0.0023 0.049 0.0024
Nuclear Periphery 0.0051 0.0019 0.0064 0.0047 0.0047 0.0048 0.0058 0.0015 0.0031 0.0014 0.0036 0.0034 0.0146 0.0017 0.0032 0.0039 0.0114 0.0045
Nucleolus 0 0 0.0008 0.0001 0 0.0001 0.0002 0 0 0.0001 0 0.0001 0.0023 0 0.0001 0.0001 0.0021 0.0001
Nucleus 0.0662 0.0177 0.0435 0.0952 0.0491 0.0652 0.0276 0.016 0.0309 0.0189 0.0513 0.0621 0.0581 0.0234 0.0509 0.0935 0.0631 0.0869
Peroxisomes 0 0.0008 0.009 0.0382 0.0002 0.007 0.0057 0 0.0001 0.0161 0 0.001 0.0094 0.0004 0.0014 0.0002 0.0135 0.0003
Punctate Nuclear 0.0009 0.0005 0.0016 0.0042 0.0004 0.0012 0.002 0.0006 0.0016 0.001 0.0016 0.0024 0.0246 0.0014 0.0013 0.0034 0.0087 0.0023
Vacuole 0.001 0.0005 0.0025 0.0002 0.0017 0.0009 0.0021 0.0002 0.0006 0.0002 0.0273 0.0006 0.0064 0.0002 0.0004 0.0005 0.0078 0.0005
Vacuole Periphery 0.0001 0.0001 0.0021 0.0001 0.0019 0.0003 0.0007 0 0.0002 0.0001 0.0204 0.0001 0.0011 0 0.0001 0.0001 0.0013 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.9414 40.8232 31.1113 38.9761 41.6062 37.0065 52.9898 46.6821 46.9668 50.1978
Translational Efficiency 0.7125 0.867 0.9083 0.8342 0.7239 0.703 0.7221 0.8337 0.7632 0.7932

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2530 513 336 1497 2110 1530 575 1539 4640 2043 911 3036

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1052.02 1316.53 1357.31 1134.27 1044.25 1134.12 1285.71 1162.13 1048.49 1179.92 1312.12 1148.39
Standard Deviation 141.79 191.91 173.20 157.80 133.87 162.98 161.27 157.21 138.30 188.14 169.33 158.12
Intensity Change Log 2 — 0.323578 0.367588 0.108602 — 0.119106 0.300098 0.154304 — 0.225340 0.334363 0.131549

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000133 0.000789 0.000691 0.000840 0.000046 0.000221 0.000577 0.003204 0.000093 0.000364 0.000619 0.002038
Bud Neck 0.001326 0.001652 0.003657 0.010272 0.001236 0.001572 0.002722 0.006664 0.001285 0.001592 0.003067 0.008443
Bud Site 0.002074 0.005809 0.004016 0.033174 0.001018 0.004484 0.005705 0.030038 0.001594 0.004817 0.005082 0.031585
Cell Periphery 0.000076 0.000129 0.000196 0.000116 0.000031 0.000054 0.000140 0.000147 0.000055 0.000073 0.000161 0.000131
Cytoplasm 0.722033* 0.664710* 0.285043* 0.428863* 0.732334* 0.754909* 0.361099* 0.519276* 0.726717* 0.732260* 0.333048* 0.474695*
Cytoplasmic Foci 0.032833 0.023896 0.004496 0.009538 0.015671 0.025092 0.002760 0.011035 0.025029 0.024792 0.003400 0.010297
Eisosomes 0.000010 0.000012 0.000023 0.000016 0.000005 0.000010 0.000015 0.000024 0.000007 0.000011 0.000018 0.000020
Endoplasmic Reticulum 0.001558 0.001351 0.003271 0.001404 0.000677 0.000875 0.002069 0.001859 0.001158 0.000995 0.002512 0.001635
Endosome 0.000762 0.001793 0.001395 0.012509 0.000350 0.001349 0.001290 0.013915 0.000574 0.001461 0.001329 0.013221
Golgi 0.000483 0.001137 0.000151 0.006289 0.000091 0.001746 0.000541 0.009893 0.000305 0.001593 0.000397 0.008116
Lipid Particles 0.000456 0.000920 0.001074 0.001038 0.000251 0.000428 0.000675 0.000944 0.000363 0.000551 0.000822 0.000991
Mitochondria 0.000690 0.003206 0.000666 0.003684 0.000379 0.000302 0.000557 0.006330 0.000548 0.001032 0.000597 0.005025
Mitotic Spindle 0.002347 0.007238 0.005874 0.186779* 0.001035 0.003456 0.002581 0.071742 0.001751 0.004406 0.003795 0.128465*
None 0.003462 0.005377 0.005992 0.007360 0.006701 0.005241 0.008912 0.009482 0.004935 0.005275 0.007835 0.008436
Nuclear Periphery 0.000249 0.000317 0.001442 0.001463 0.000213 0.000161 0.000909 0.001113 0.000232 0.000200 0.001106 0.001286
Nuclear Periphery Foci 0.001227 0.004171 0.002482 0.002165 0.000481 0.001655 0.002179 0.002340 0.000888 0.002287 0.002291 0.002254
Nucleolus 0.000296 0.000172 0.000216 0.000843 0.000154 0.000515 0.000230 0.000348 0.000232 0.000429 0.000225 0.000592
Nucleus 0.209269* 0.246359* 0.600424* 0.183226* 0.229472* 0.166903* 0.549094* 0.216739* 0.218456* 0.186855* 0.568026* 0.200214*
Peroxisomes 0.000285 0.000371 0.000106 0.001064 0.000069 0.000729 0.000114 0.000534 0.000187 0.000640 0.000111 0.000796
Vacuole 0.020182 0.030389 0.078656 0.091862 0.009666 0.029992 0.055861 0.087681 0.015400 0.030092 0.064268 0.089743
Vacuole Periphery 0.000249 0.000199 0.000129 0.017495 0.000120 0.000304 0.001971 0.006691 0.000190 0.000278 0.001292 0.012018

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.54 -7.24 -5.21 0.46 2.44 -5.39 -13.57 -3.04 -2.42 -0.77 -4.22 -13.88 -4.26 -2.51 0.13
Bud Neck -3.85 -10.66 -7.17 -5.50 -0.19 -1.03 -8.12 -7.94 -5.83 0.01 -1.90 -12.73 -10.24 -7.84 -0.58
Bud Site -2.63 -6.79 -6.28 -1.96 -2.70 -5.01 -4.19 -8.43 -4.98 -3.33 -5.17 -5.74 -10.38 -5.36 -4.03
Cell Periphery -2.93 -7.27 -1.57 2.64 8.64 -7.45 -13.35 -16.67 -9.47 7.25 -2.11 -11.59 -4.62 -5.32 10.96
Cytoplasm 8.25 42.02 50.17 22.85 -8.92 -0.33 47.54 42.85 39.78 -12.48 3.25 63.18 65.30 49.71 -14.50
Cytoplasmic Foci 2.87 18.35 21.09 9.48 1.12 -6.04 12.62 9.16 12.82 -5.96 -0.70 23.07 22.32 16.32 -3.19
Eisosomes -3.61 -11.01 -18.23 -6.96 3.26 -7.43 -15.28 -18.78 -10.59 -0.24 -6.58 -17.92 -25.58 -13.72 1.89
Endoplasmic Reticulum 0.01 -5.68 -0.67 -1.17 6.86 -2.31 -10.75 -7.42 -5.67 5.85 0.31 -9.32 -3.16 -6.54 9.01
Endosome -2.98 -6.67 -3.10 -0.78 -0.55 -3.59 -5.84 -3.87 -2.27 -2.37 -3.92 -7.88 -4.90 -2.51 -2.44
Golgi -1.09 1.63 -1.02 0.20 -2.20 -2.35 -1.78 -2.15 -0.01 -1.86 -2.23 0.56 -2.31 0.31 -2.52
Lipid Particles -3.02 -11.51 -13.86 -2.20 2.46 -2.41 -13.30 -18.08 -10.30 -0.54 -3.06 -17.08 -22.20 -9.89 1.03
Mitochondria -1.17 -0.60 -1.75 0.77 -2.21 0.86 -3.35 -2.87 -3.17 -2.03 -0.68 -1.35 -3.15 -1.34 -2.89
Mitotic Spindle -1.77 -2.47 -11.35 -9.26 -7.84 -2.12 -3.03 -9.35 -8.33 -6.26 -2.39 -3.76 -14.57 -13.11 -10.38
None -3.16 -2.25 -10.73 -7.71 -7.33 3.52 -1.20 -2.39 -6.45 -0.68 0.12 -3.22 -9.70 -9.94 -4.41
Nuclear Periphery -4.80 -18.75 -29.31 -25.41 -3.74 1.11 -19.57 -28.89 -30.04 -1.78 -1.09 -26.82 -40.46 -39.82* -4.75
Nuclear Periphery Foci -6.32 -5.23 -4.33 5.24 3.55 -8.06 -8.90 -11.51 -2.00 3.35 -8.86 -9.78 -11.13 2.68 4.97
Nucleolus 0.97 0.34 -1.57 -3.05 -2.84 -1.37 -2.60 -5.03 0.73 -2.14 -0.93 -0.27 -2.56 0.09 -3.57
Nucleus -5.02 -22.95 -12.11 -3.05 16.71 10.20 -28.67 -6.10 -14.84 23.84 5.39 -37.44 -13.10 -15.82 28.86
Peroxisomes -0.35 1.90 0.42 0.70 -1.38 -2.27 -2.28 -4.52 1.86 -1.98 -1.92 1.49 -0.36 1.82 -2.14
Vacuole -6.13 -12.74 -33.89 -24.10 -7.73 -13.55 -16.05 -34.68 -27.82 -10.70 -13.20 -20.12 -48.31 -38.43 -13.56
Vacuole Periphery 0.82 1.26 -2.51 -2.60 -2.62 -1.58 -0.97 -2.15 -1.77 -0.85 -0.77 -0.78 -3.25 -3.07 -2.33
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YGR017W

YGR017W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ygr017w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available