Standard name
Human Ortholog
Description Gamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.08 0.09 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.99 0.96 0.95 0.98 0.89 0.89 0.88 0.9 0.86 0.89 1.0 0.99 1.0 0.98 0.97 0.98 0.91 0.88 0.87 0.96 0.94 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0.08 0.1 0.09 0.06 0.06 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 3 2 2 0 0 0 0 0 0 0 0 2 0 0 0
Bud 0 0 1 0 0 0 0 1 1 1 1 1 2 0 0 0 0 2 5 8 7 1 8 11
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 2 1 0 5 4 11 6 8 13 15 7 5 5 3 14 23 8 0 0 0 0 0 0
Cytoplasm 173 134 140 92 162 193 223 222 168 140 138 125 203 263 269 503 460 364 166 117 118 136 241 187
Endoplasmic Reticulum 0 1 0 3 6 2 21 25 17 10 9 9 0 2 0 21 23 16 0 0 2 1 0 4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 2 0 0 0 0 0 1 0
Mitochondria 0 0 0 0 0 0 0 0 3 2 5 1 0 0 0 2 1 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 1 0 0 1 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Vac/Vac Membrane 2 2 1 1 0 0 0 0 0 0 1 0 0 1 0 3 5 5 0 1 1 2 0 3
Unique Cell Count 175 137 141 96 170 196 251 249 192 156 161 140 203 267 269 512 472 370 183 133 137 143 256 212
Labelled Cell Count 179 139 143 96 173 199 255 254 198 171 173 145 210 271 272 547 514 395 183 133 137 143 256 212


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.7 22.0 28.1 62.9 70.6 85.5 84.4 80.6 75.8 61.8 69.9 71.2 25.5 28.4 28.7 56.5 66.9 66.9 19.8 21.1 20.4
Std Deviation (1e-4) 9.9 14.0 14.6 24.6 24.8 29.2 25.0 28.2 30.0 23.0 26.8 26.6 10.7 11.4 10.9 21.5 25.5 30.6 9.7 9.4 8.5
Intensity Change (Log2) – – – 1.16 1.33 1.6 1.58 1.52 1.43 1.13 1.31 1.34 -0.14 0.01 0.03 1.01 1.25 1.25 -0.51 -0.42 -0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 3.1 3.3 0 0 0 0 0 2.2 0
Cytoplasm -1.8 -2.1 -0.7 -3.8 -3.7 -4.0 -3.5 -4.4 -3.6 1.2 -0.7 1.4 -0.9 -1.3 -0.8
Endoplasmic Reticulum 0 0 0 3.5 3.9 3.6 0 0 0 0 0 0 0 2.7 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.0399 15.2519 11.9146 9.4738 9.2641 11.8763 13.482 15.6881 13.8552 14.7089 13.4744 14.4333 12.9828 16.2322 14.2182 14.8856 12.8069 15.5977
Actin 0.0142 0.001 0.0085 0.0002 0.0303 0.006 0.018 0.0013 0.0087 0.0249 0.0066 0.0159 0.049 0.0007 0.0239 0.0184 0.0247 0.0017
Bud 0.002 0.0002 0.003 0.0002 0.0003 0.0003 0.0009 0.0031 0.0007 0.0007 0.0036 0.0006 0.0006 0.0003 0.0006 0.0004 0.0003 0.0002
Bud Neck 0.0014 0.0002 0.0007 0.0004 0.0006 0.001 0.0032 0.0002 0.0009 0.0005 0.0005 0.001 0.0019 0.0001 0.0008 0.0008 0.0009 0.0011
Bud Periphery 0.0014 0.0001 0.0016 0 0.0001 0.0003 0.0007 0.0018 0.0003 0.0004 0.0014 0.0003 0.0007 0.0001 0.0004 0.0007 0.0004 0.0001
Bud Site 0.0031 0.0011 0.0039 0.0001 0.0003 0.0004 0.0068 0.0031 0.0011 0.0013 0.0015 0.0007 0.0018 0.0004 0.0033 0.0053 0.0019 0.0001
Cell Periphery 0.0005 0.0001 0.0002 0 0 0.0001 0.0004 0.0001 0.0001 0 0.0001 0.0001 0.0002 0 0.0002 0.0008 0.0001 0
Cytoplasm 0.8484 0.9828 0.9376 0.9838 0.8965 0.9482 0.8616 0.9688 0.9331 0.8599 0.8773 0.9377 0.8328 0.9892 0.9188 0.9376 0.8441 0.9658
Cytoplasmic Foci 0.0207 0.0014 0.012 0.0026 0.008 0.0045 0.027 0.0105 0.0153 0.0303 0.0233 0.0097 0.0315 0.0021 0.0094 0.0031 0.0154 0.0061
Eisosomes 0.0003 0 0.0001 0 0.0003 0.0001 0.0003 0 0 0.0001 0 0.0001 0.0003 0 0.0003 0.001 0.0002 0
Endoplasmic Reticulum 0.0073 0.001 0.0013 0.0015 0.0019 0.0042 0.0069 0.0006 0.0021 0.0014 0.0035 0.0046 0.0102 0.0006 0.0043 0.0031 0.0032 0.0021
Endosome 0.0223 0.0007 0.0032 0.0018 0.0078 0.0074 0.0199 0.0022 0.0098 0.0092 0.0169 0.007 0.0201 0.0004 0.0056 0.0045 0.0145 0.0035
Golgi 0.0074 0.0001 0.0014 0.0001 0.0052 0.0013 0.0078 0.0004 0.0059 0.0039 0.0049 0.003 0.0044 0.0001 0.0025 0.0028 0.0109 0.0006
Lipid Particles 0.0118 0 0.0027 0 0.0088 0.0008 0.0056 0.0002 0.0019 0.0099 0.0014 0.0018 0.0033 0 0.0035 0.0034 0.0244 0.0001
Mitochondria 0.0088 0.0001 0.0009 0.0001 0.0019 0.0068 0.0071 0.0002 0.0062 0.0043 0.0025 0.001 0.0022 0.0001 0.0018 0.0088 0.0296 0.001
None 0.0086 0.0067 0.0071 0.0012 0.0038 0.0014 0.0142 0.0023 0.0028 0.0028 0.0031 0.0054 0.0076 0.0023 0.0082 0.0026 0.0025 0.0092
Nuclear Periphery 0.0065 0.0008 0.0016 0.0013 0.0081 0.0025 0.0026 0.0006 0.0014 0.0019 0.0052 0.002 0.0107 0.0005 0.0025 0.001 0.0017 0.0013
Nucleolus 0.0004 0 0.0005 0 0.0004 0 0.0004 0 0.0001 0.0002 0.0001 0.0001 0.0004 0 0.0003 0.0001 0.0015 0
Nucleus 0.0121 0.0027 0.0077 0.0051 0.0061 0.0098 0.0066 0.0035 0.0036 0.0056 0.0062 0.0047 0.011 0.0025 0.005 0.0023 0.0036 0.0056
Peroxisomes 0.0093 0.0001 0.0027 0 0.0093 0.0016 0.0023 0.0002 0.0031 0.0381 0.0382 0.0016 0.0027 0 0.0041 0.0009 0.0161 0.0002
Punctate Nuclear 0.0057 0.0002 0.0023 0.0004 0.0065 0.0007 0.0041 0.0004 0.001 0.0029 0.0023 0.0016 0.006 0.0002 0.0034 0.0003 0.0016 0.0008
Vacuole 0.0064 0.0007 0.0008 0.001 0.0029 0.002 0.003 0.0004 0.0016 0.0013 0.0012 0.001 0.0018 0.0002 0.0007 0.0016 0.0015 0.0005
Vacuole Periphery 0.0014 0.0001 0.0002 0.0002 0.0008 0.0007 0.0006 0.0001 0.0003 0.0004 0.0004 0.0003 0.0007 0 0.0003 0.0006 0.0006 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.378 39.0092 37.217 49.1805 36.3715 54.5166 71.8411 72.0188 89.9508 69.9724
Translational Efficiency 1.233 1.6218 1.5474 1.4826 1.7722 1.6132 1.5938 1.6099 1.5042 1.5577

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
621 1439 1420 1753 401 2120 200 172 1022 3559 1620 1925

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1918.83 1592.32 1702.68 3230.58 1517.59 1533.83 3551.20 3943.30 1761.40 1557.48 1930.89 3294.26
Standard Deviation 772.09 554.89 506.47 1533.40 535.02 533.79 1377.05 1930.59 716.18 543.18 910.34 1586.06
Intensity Change Log 2 — -0.269097 -0.172420 0.751566 — 0.015357 1.226525 1.377621 — -0.136519 0.612477 1.061847

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000229 0.000158 0.000141 0.000493 0.000604 0.000125 0.000446 0.000164 0.000376 0.000138 0.000179 0.000464
Bud Neck 0.000893 0.000644 0.001971 0.002250 0.000735 0.001088 0.005471 0.003486 0.000831 0.000909 0.002403 0.002360
Bud Site 0.011581 0.007111 0.004749 0.015070 0.011513 0.007945 0.007429 0.020201 0.011554 0.007608 0.005079 0.015529
Cell Periphery 0.001426 0.000241 0.000140 0.005517 0.000695 0.000124 0.005825 0.003825 0.001139 0.000172 0.000842 0.005365
Cytoplasm 0.882842* 0.918149* 0.818680* 0.842543* 0.893159* 0.903745* 0.678805* 0.790086* 0.886890* 0.909569* 0.801412* 0.837856*
Cytoplasmic Foci 0.044099 0.014561 0.009349 0.010965 0.027531 0.017234 0.037211 0.011586 0.037599 0.016153 0.012789 0.011021
Eisosomes 0.000002 0.000002 0.000005 0.000006 0.000004 0.000002 0.000006 0.000004 0.000003 0.000002 0.000005 0.000006
Endoplasmic Reticulum 0.000921 0.000412 0.000426 0.000147 0.001160 0.000194 0.000181 0.000040 0.001015 0.000282 0.000396 0.000137
Endosome 0.002250 0.001377 0.001870 0.001352 0.002336 0.002533 0.002175 0.000472 0.002284 0.002065 0.001908 0.001273
Golgi 0.012233 0.002093 0.000571 0.004368 0.004729 0.001875 0.011593 0.001459 0.009289 0.001963 0.001932 0.004108
Lipid Particles 0.003141 0.000488 0.000595 0.009505 0.001131 0.000371 0.015383 0.011714 0.002352 0.000418 0.002421 0.009702
Mitochondria 0.000063 0.000233 0.000204 0.001303 0.000106 0.000293 0.000079 0.000042 0.000080 0.000269 0.000188 0.001191
Mitotic Spindle 0.002666 0.004640 0.006525 0.003989 0.002583 0.007822 0.001345 0.000926 0.002633 0.006536 0.005886 0.003716
None 0.005316 0.006201 0.006172 0.002278 0.003675 0.006794 0.001865 0.002868 0.004672 0.006554 0.005641 0.002331
Nuclear Periphery 0.000062 0.000087 0.000301 0.000151 0.000068 0.000089 0.000502 0.000182 0.000064 0.000088 0.000325 0.000153
Nuclear Periphery Foci 0.005305 0.002049 0.001566 0.002269 0.004924 0.001003 0.010320 0.004144 0.005156 0.001426 0.002647 0.002437
Nucleolus 0.000055 0.000215 0.000299 0.000104 0.000098 0.000186 0.000104 0.000306 0.000072 0.000198 0.000275 0.000122
Nucleus 0.013307 0.030871 0.076166 0.037788 0.026255 0.027829 0.064037 0.036207 0.018387 0.029059 0.074668 0.037647
Peroxisomes 0.000174 0.000133 0.000070 0.000206 0.000103 0.000268 0.000058 0.000103 0.000146 0.000213 0.000069 0.000197
Vacuole 0.009545 0.008519 0.068496 0.057053 0.014754 0.018887 0.153720 0.111327 0.011589 0.014695 0.079017 0.061902
Vacuole Periphery 0.003890 0.001816 0.001704 0.002641 0.003836 0.001592 0.003446 0.000857 0.003869 0.001682 0.001919 0.002482

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.53 1.03 -2.75 -3.77 -3.51 2.14 -0.06 1.65 -2.90 3.67 2.57 1.49 -0.79 -4.12 -2.87
Bud Neck 1.35 -7.37 -7.90 -9.77 1.81 -3.22 -8.18 -5.47 -4.93 1.92 -1.43 -10.00 -10.10 -9.59 2.72
Bud Site 2.58 3.70 -2.02 -5.80 -7.72 1.36 0.29 -0.88 -1.57 -1.08 2.71 3.61 -2.23 -6.15 -7.32
Cell Periphery 4.93 5.34 -5.62 -8.56 -8.75 3.21 -7.37 -4.43 -5.59 2.39 5.98 1.82 -6.79 -9.28 -7.77
Cytoplasm -4.41 12.20 8.01 16.02 -5.34 -0.42 12.81 8.00 8.53 -2.94 -3.08 16.91 11.23 17.01 -6.54
Cytoplasmic Foci 8.51 9.60 9.23 1.18 -1.20 3.49 -3.53 2.94 0.17 5.45 8.92 8.67 10.10 2.68 1.58
Eisosomes 0.75 -2.36 -2.49 -2.72 -0.19 3.01 -3.52 -0.74 -4.34 3.01 2.63 -2.23 -2.03 -2.81 0.02
Endoplasmic Reticulum 1.92 2.11 3.39 2.28 6.14 4.27 4.27 4.93 4.84 4.47 4.17 3.64 5.32 2.88 6.39
Endosome 2.08 0.51 3.63 1.03 4.26 -0.24 0.15 3.58 4.29 4.52 0.67 0.61 4.27 3.21 4.90
Golgi 6.07 7.04 5.08 -1.52 -3.76 3.75 -3.05 4.58 1.20 5.73 6.91 7.10 5.16 -1.72 -1.99
Lipid Particles 5.07 4.38 -6.84 -11.90 -11.44 2.58 -7.66 -4.70 -4.95 0.31 5.74 -1.11 -9.44 -13.05 -9.03
Mitochondria -3.35 -8.58 -2.00 -1.59 -1.65 -2.09 0.19 1.46 2.55 1.55 -3.39 -7.44 -1.96 -1.47 -1.65
Mitotic Spindle -1.74 -4.97 -1.38 0.46 3.11 -4.27 1.68 1.79 5.31 0.48 -4.53 -5.02 -1.37 2.48 3.00
None -0.64 0.15 2.79 7.09 4.46 -2.93 2.64 1.34 4.67 -1.14 -1.95 -0.06 3.26 8.79 4.28
Nuclear Periphery -1.26 -10.31 -6.40 -2.90 6.80 -2.22 -9.01 -4.96 -4.16 4.02 -2.51 -12.12 -7.63 -4.57 7.74
Nuclear Periphery Foci 3.68 3.94 3.43 -0.99 -2.00 4.43 -4.19 -0.52 -4.35 3.44 5.90 3.21 3.87 -5.86 1.42
Nucleolus -1.91 -11.27 -4.40 1.26 8.59 -2.04 -1.64 -1.18 -0.54 -1.06 -2.96 -10.67 -2.56 1.70 5.97
Nucleus -11.42 -23.77 -17.57 -7.04 11.25 -1.09 -7.83 -3.49 -3.23 3.14 -8.05 -22.71 -14.29 -9.20 11.73
Peroxisomes 0.48 0.96 0.09 -0.46 -1.09 -3.43 1.18 -0.43 3.12 -1.44 -1.29 1.05 -0.19 0.95 -1.16
Vacuole -0.07 -17.74 -18.19 -18.76 3.91 -2.71 -9.97 -8.17 -7.74 0.41 -3.85 -19.53 -18.83 -17.34 4.17
Vacuole Periphery 1.36 1.62 1.26 -0.30 -1.16 1.37 0.57 1.84 2.22 3.56 1.96 1.98 1.73 -0.53 -0.60
Standard name
Human Ortholog
Description Gamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Uga1

Uga1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Uga1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available