Standard name | |
---|---|
Human Ortholog | |
Description | Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; non-essential even when paralog is also deleted; deletion has minimal affections on ribosome biosynthesis; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 3 | 4 | 6 | 12 | 16 | 21 | 32 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 4 | 1 | 1 | 6 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 1 | 0 | 5 | 6 | 16 | 11 | 11 | 38 | 22 | 30 | 0 | 2 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | 125 | 233 | 216 | 404 | 616 | 527 | 538 | 508 | 430 | 543 | 383 | 519 | 303 | 437 | 525 | 141 | 164 | 109 | 116 | 215 | 199 | 245 | 362 | 431 |
Endoplasmic Reticulum | 0 | 0 | 1 | 2 | 7 | 19 | 123 | 214 | 117 | 151 | 90 | 132 | 9 | 15 | 16 | 7 | 4 | 4 | 1 | 1 | 5 | 5 | 22 | 16 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 8 | 15 | 5 | 17 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 0 | 0 | 1 | 0 | 0 | 5 | 5 | 4 | 7 | 7 | 13 | 28 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 3 | 2 | 2 | 3 | 1 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 7 | 7 | 5 |
Unique Cell Count | 126 | 233 | 218 | 406 | 627 | 553 | 676 | 738 | 564 | 735 | 512 | 703 | 313 | 454 | 542 | 141 | 164 | 109 | 122 | 223 | 211 | 270 | 409 | 469 |
Labelled Cell Count | 128 | 235 | 221 | 406 | 629 | 561 | 696 | 760 | 592 | 775 | 540 | 767 | 315 | 460 | 548 | 148 | 170 | 114 | 122 | 223 | 211 | 270 | 409 | 469 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 153.0 | 189.9 | 124.2 | 127.7 | 93.3 | 60.8 | 54.7 | 53.3 | 49.6 | 49.2 | 44.4 | 43.6 | 152.5 | 151.0 | 150.4 | 196.7 | 211.0 | 203.9 | 174.4 | 182.4 | 186.2 |
Std Deviation (1e-4) | 40.0 | 33.8 | 29.8 | 29.1 | 20.6 | 13.6 | 12.7 | 13.1 | 11.2 | 11.7 | 11.2 | 10.5 | 35.6 | 34.4 | 34.3 | 53.4 | 58.7 | 49.2 | 45.4 | 49.0 | 43.7 |
Intensity Change (Log2) | – | – | – | 0.04 | -0.41 | -1.03 | -1.18 | -1.22 | -1.32 | -1.34 | -1.49 | -1.51 | 0.3 | 0.28 | 0.28 | 0.66 | 0.76 | 0.71 | 0.49 | 0.55 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 2.7 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | -0.9 | -2.5 | -6.9 | -9.2 | -7.6 | -8.1 | -7.8 | -8.1 | -1.7 | -2.1 | -1.8 | 1.1 | 1.2 | 1.0 |
Endoplasmic Reticulum | 0 | 0 | 2.3 | 6.6 | 8.9 | 7.1 | 7.1 | 6.4 | 6.7 | 0 | 2.3 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 142.2067 | 186.3671 | 175.9856 | 164.3058 | 182.4247 | 173.8979 | 130.9823 | 175.8183 | 167.0233 | 148.358 | 122.002 | 145.2794 | 127.4352 | 195.2384 | 173.3512 | 159.9342 | 174.8711 | 176.1078 |
Actin | 0.0013 | 0.0003 | 0.0004 | 0.0002 | 0.0005 | 0.0028 | 0.0119 | 0.0003 | 0.0005 | 0.0032 | 0.018 | 0.0034 | 0.033 | 0.0003 | 0.0097 | 0.0045 | 0.0002 | 0.002 |
Bud | 0.0015 | 0.0007 | 0.0011 | 0.0012 | 0.0008 | 0.0017 | 0.0032 | 0.0008 | 0.0011 | 0.0032 | 0.0053 | 0.0056 | 0.0016 | 0.0007 | 0.0018 | 0.004 | 0.0008 | 0.0083 |
Bud Neck | 0.0009 | 0.0006 | 0.001 | 0.0005 | 0.0016 | 0.0035 | 0.0008 | 0.0004 | 0.0011 | 0.001 | 0.0017 | 0.0018 | 0.0169 | 0.0004 | 0.0043 | 0.0008 | 0.0004 | 0.0011 |
Bud Periphery | 0.0023 | 0.0007 | 0.001 | 0.0011 | 0.0003 | 0.0029 | 0.0035 | 0.0006 | 0.0007 | 0.0027 | 0.0058 | 0.0031 | 0.002 | 0.0003 | 0.0008 | 0.0029 | 0.0004 | 0.0024 |
Bud Site | 0.0007 | 0.0005 | 0.0005 | 0.0004 | 0.0002 | 0.0002 | 0.0033 | 0.0005 | 0.0005 | 0.0009 | 0.002 | 0.001 | 0.0142 | 0.0009 | 0.0133 | 0.0032 | 0.0001 | 0.0005 |
Cell Periphery | 0.0601 | 0.0441 | 0.0506 | 0.0384 | 0.0066 | 0.0156 | 0.0351 | 0.0082 | 0.0266 | 0.0078 | 0.0071 | 0.0109 | 0.051 | 0.0182 | 0.0192 | 0.0172 | 0.0087 | 0.0009 |
Cytoplasm | 0.5544 | 0.8151 | 0.7064 | 0.4839 | 0.78 | 0.733 | 0.6531 | 0.9213 | 0.7899 | 0.5658 | 0.6667 | 0.6462 | 0.5169 | 0.9232 | 0.8311 | 0.626 | 0.9349 | 0.902 |
Cytoplasmic Foci | 0.0065 | 0.0006 | 0.0027 | 0.0012 | 0.0032 | 0.0023 | 0.0267 | 0.0007 | 0.0102 | 0.004 | 0.0126 | 0.0055 | 0.02 | 0.0012 | 0.0091 | 0.0034 | 0.0018 | 0.0029 |
Eisosomes | 0.001 | 0.0022 | 0 | 0 | 0 | 0.0001 | 0.0009 | 0 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0008 | 0.0002 | 0.0002 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0644 | 0.0112 | 0.0286 | 0.0035 | 0.0124 | 0.0953 | 0.0228 | 0.004 | 0.0063 | 0.0037 | 0.0102 | 0.0481 | 0.0216 | 0.0022 | 0.0035 | 0.0021 | 0.001 | 0.0024 |
Endosome | 0.0096 | 0.0014 | 0.0038 | 0.0052 | 0.0083 | 0.0108 | 0.0101 | 0.0015 | 0.0058 | 0.0212 | 0.0515 | 0.0226 | 0.0262 | 0.0006 | 0.0018 | 0.0156 | 0.0034 | 0.0091 |
Golgi | 0.0022 | 0.0003 | 0.0005 | 0.0002 | 0.0003 | 0.0035 | 0.0038 | 0.0002 | 0.0008 | 0.0106 | 0.0248 | 0.0046 | 0.0091 | 0.0001 | 0.0015 | 0.0071 | 0.0001 | 0.0008 |
Lipid Particles | 0.0063 | 0.0025 | 0.0026 | 0.0037 | 0.0007 | 0.0038 | 0.0114 | 0.0005 | 0.0021 | 0.0014 | 0.0044 | 0.0013 | 0.0182 | 0.0017 | 0.0027 | 0.0013 | 0.0004 | 0.0001 |
Mitochondria | 0.0125 | 0.0008 | 0.0014 | 0.0016 | 0.0003 | 0.0098 | 0.0161 | 0.003 | 0.002 | 0.0212 | 0.0396 | 0.021 | 0.0206 | 0.0003 | 0.0027 | 0.015 | 0.0002 | 0.0083 |
None | 0.0059 | 0.0004 | 0.0011 | 0.0005 | 0.0003 | 0.0007 | 0.0148 | 0.0002 | 0.0027 | 0.0014 | 0.0134 | 0.0024 | 0.019 | 0.0012 | 0.0035 | 0.0009 | 0.0005 | 0.0008 |
Nuclear Periphery | 0.0378 | 0.0066 | 0.0091 | 0.0073 | 0.0188 | 0.0167 | 0.0233 | 0.0021 | 0.0086 | 0.0118 | 0.0313 | 0.0857 | 0.0424 | 0.004 | 0.0067 | 0.0147 | 0.0069 | 0.008 |
Nucleolus | 0.0227 | 0.0005 | 0.0038 | 0.0026 | 0.0004 | 0.0005 | 0.0075 | 0.0001 | 0.0009 | 0.0025 | 0.0054 | 0.0091 | 0.0121 | 0.001 | 0.0044 | 0.0039 | 0.0002 | 0.0002 |
Nucleus | 0.0131 | 0.0032 | 0.0049 | 0.0088 | 0.0122 | 0.0061 | 0.0134 | 0.0019 | 0.005 | 0.0077 | 0.0076 | 0.0178 | 0.0229 | 0.0034 | 0.0057 | 0.0133 | 0.0036 | 0.0086 |
Peroxisomes | 0.0006 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0014 | 0 | 0.0003 | 0.0006 | 0.0014 | 0.0007 | 0.0108 | 0 | 0.0061 | 0.0005 | 0 | 0.0003 |
Punctate Nuclear | 0.0011 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0024 | 0 | 0.0007 | 0.0002 | 0.0011 | 0.0011 | 0.0079 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
Vacuole | 0.171 | 0.1019 | 0.1613 | 0.3688 | 0.1308 | 0.0769 | 0.1092 | 0.0484 | 0.1174 | 0.2467 | 0.0688 | 0.0666 | 0.1066 | 0.0375 | 0.0619 | 0.1954 | 0.0289 | 0.0261 |
Vacuole Periphery | 0.0239 | 0.0062 | 0.0191 | 0.0707 | 0.0221 | 0.0138 | 0.0254 | 0.0053 | 0.0168 | 0.0824 | 0.0209 | 0.0414 | 0.0263 | 0.0028 | 0.0098 | 0.0682 | 0.0077 | 0.0151 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1958.2555 | 2149.9918 | 2243.453 | 2062.0054 | 2412.2951 | 1898.2984 | 1733.6295 | 1582.0224 | 1728.0096 | 1777.1372 |
Translational Efficiency | 1.5698 | 1.4434 | 1.5455 | 1.2897 | 1.643 | 1.6888 | 1.5459 | 1.7532 | 1.6207 | 1.7016 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1154 | 1607 | 724 | 1153 | 1178 | 1533 | 2053 | 1018 | 2332 | 3140 | 2777 | 2171 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 7777.57 | 7370.16 | 13351.85 | 11659.53 | 7927.58 | 7835.78 | 13123.84 | 11475.53 | 7853.35 | 7597.48 | 13183.29 | 11573.25 |
Standard Deviation | 1903.42 | 2217.52 | 2742.53 | 2909.96 | 2213.94 | 1853.35 | 2753.96 | 2990.76 | 2067.48 | 2061.02 | 2752.80 | 2949.55 |
Intensity Change Log 2 | — | -0.077624 | 0.779648 | 0.584118 | — | -0.016804 | 0.727237 | 0.533608 | — | -0.046603 | 0.753431 | 0.558843 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000071 | 0.000069 | 0.000124 | 0.000412 | 0.000040 | 0.000092 | 0.000084 | 0.000392 | 0.000055 | 0.000080 | 0.000095 | 0.000402 |
Bud Neck | 0.000500 | 0.000325 | 0.005513 | 0.001439 | 0.000301 | 0.000560 | 0.000389 | 0.001714 | 0.000399 | 0.000440 | 0.001724 | 0.001568 |
Bud Site | 0.000846 | 0.001271 | 0.002698 | 0.006335 | 0.000991 | 0.002105 | 0.002494 | 0.009606 | 0.000919 | 0.001678 | 0.002547 | 0.007869 |
Cell Periphery | 0.020688 | 0.011328 | 0.025262 | 0.109702* | 0.024258 | 0.011672 | 0.073445 | 0.086548 | 0.022491 | 0.011496 | 0.060883 | 0.098845 |
Cytoplasm | 0.636522* | 0.729314* | 0.225640* | 0.248412* | 0.659357* | 0.700270* | 0.442248* | 0.192297* | 0.648057* | 0.715134* | 0.385776* | 0.222099* |
Cytoplasmic Foci | 0.009476 | 0.006443 | 0.014879 | 0.003379 | 0.006124 | 0.007841 | 0.003592 | 0.004092 | 0.007783 | 0.007125 | 0.006535 | 0.003713 |
Eisosomes | 0.000003 | 0.000001 | 0.000008 | 0.000012 | 0.000002 | 0.000001 | 0.000003 | 0.000011 | 0.000002 | 0.000001 | 0.000004 | 0.000012 |
Endoplasmic Reticulum | 0.000272 | 0.000437 | 0.000480 | 0.001286 | 0.000705 | 0.000436 | 0.001766 | 0.000822 | 0.000491 | 0.000437 | 0.001431 | 0.001068 |
Endosome | 0.000296 | 0.000313 | 0.000689 | 0.000534 | 0.000309 | 0.000513 | 0.000513 | 0.002663 | 0.000302 | 0.000411 | 0.000559 | 0.001532 |
Golgi | 0.061619 | 0.044610 | 0.051373 | 0.024671 | 0.057704 | 0.050339 | 0.017791 | 0.023599 | 0.059641 | 0.047407 | 0.026546 | 0.024168 |
Lipid Particles | 0.009048 | 0.005176 | 0.017635 | 0.040681 | 0.014587 | 0.007355 | 0.024126 | 0.049345 | 0.011846 | 0.006240 | 0.022434 | 0.044744 |
Mitochondria | 0.000224 | 0.000494 | 0.000273 | 0.000545 | 0.000120 | 0.000311 | 0.000323 | 0.003380 | 0.000171 | 0.000404 | 0.000310 | 0.001874 |
Mitotic Spindle | 0.000439 | 0.000372 | 0.001640 | 0.000534 | 0.000389 | 0.000759 | 0.000292 | 0.001354 | 0.000414 | 0.000561 | 0.000643 | 0.000919 |
None | 0.004752 | 0.007238 | 0.001085 | 0.002095 | 0.004196 | 0.003764 | 0.000932 | 0.001367 | 0.004471 | 0.005542 | 0.000972 | 0.001754 |
Nuclear Periphery | 0.001281 | 0.000644 | 0.006171 | 0.001585 | 0.000953 | 0.000591 | 0.000799 | 0.001017 | 0.001115 | 0.000618 | 0.002200 | 0.001319 |
Nuclear Periphery Foci | 0.013693 | 0.009994 | 0.018576 | 0.011090 | 0.013716 | 0.010944 | 0.005485 | 0.007452 | 0.013705 | 0.010458 | 0.008898 | 0.009384 |
Nucleolus | 0.000260 | 0.000176 | 0.000301 | 0.000199 | 0.000106 | 0.000158 | 0.000151 | 0.000278 | 0.000182 | 0.000167 | 0.000190 | 0.000236 |
Nucleus | 0.017162 | 0.012366 | 0.023942 | 0.008414 | 0.011969 | 0.014412 | 0.003525 | 0.005700 | 0.014539 | 0.013365 | 0.008848 | 0.007141 |
Peroxisomes | 0.000006 | 0.000029 | 0.000014 | 0.000093 | 0.000010 | 0.000014 | 0.000281 | 0.000216 | 0.000008 | 0.000021 | 0.000211 | 0.000151 |
Vacuole | 0.093244 | 0.075109 | 0.444017* | 0.445853* | 0.107644 | 0.108917 | 0.339170* | 0.537087* | 0.100518 | 0.091615 | 0.366505* | 0.488633* |
Vacuole Periphery | 0.129599* | 0.094290 | 0.159680* | 0.092730 | 0.096521 | 0.078946 | 0.082591 | 0.071061 | 0.112890* | 0.086799 | 0.102689* | 0.082569 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.01 | -5.28 | -3.13 | -3.12 | -2.44 | -2.88 | -3.66 | -4.82 | -3.94 | -4.09 | -2.13 | -4.42 | -5.12 | -4.67 | -4.36 |
Bud Neck | 1.55 | -10.41 | -6.86 | -7.26 | 8.66 | -4.78 | -3.03 | -8.29 | -5.97 | -7.56 | -1.98 | -10.01 | -10.83 | -9.24 | 2.46 |
Bud Site | -2.15 | -4.92 | -7.50 | -6.91 | -3.38 | -4.21 | -4.40 | -5.53 | -4.72 | -4.56 | -4.67 | -6.56 | -8.33 | -7.32 | -6.01 |
Cell Periphery | 5.72 | -1.00 | -22.48 | -26.14* | -21.73 | 7.84 | -16.64 | -14.99 | -19.44 | -2.12 | 9.60 | -16.93 | -26.60 | -32.31* | -11.13 |
Cytoplasm | -7.12 | 28.12 | 27.97 | 39.81 | -2.22 | -2.72 | 17.38 | 34.38 | 40.82 | 20.23 | -6.98 | 27.16 | 43.73 | 56.65 | 16.75 |
Cytoplasmic Foci | 3.72 | -4.55 | 6.89 | 3.53 | 7.69 | -2.88 | 4.70 | 2.12 | 4.29 | -0.97 | 0.99 | 0.54 | 6.45 | 5.60 | 4.99 |
Eisosomes | 3.41 | -7.98 | -3.83 | -4.28 | -1.38 | 1.87 | -5.57 | -6.46 | -6.72 | -5.51 | 3.86 | -8.49 | -6.34 | -6.83 | -4.71 |
Endoplasmic Reticulum | -2.13 | -1.44 | -8.30 | -6.08 | -5.58 | 2.17 | -6.09 | -0.68 | -3.93 | 6.27 | 0.74 | -8.07 | -5.75 | -7.27 | 2.89 |
Endosome | -0.38 | -9.41 | -5.73 | -5.17 | 4.64 | -4.83 | -6.64 | -3.25 | -2.89 | -2.94 | -3.89 | -10.76 | -3.64 | -3.24 | -2.74 |
Golgi | 5.40 | 4.95 | 13.92 | 8.88 | 9.91 | 2.57 | 15.70 | 12.52 | 10.42 | -3.05 | 5.62 | 18.30 | 18.66 | 13.67 | 1.63 |
Lipid Particles | 4.15 | -5.91 | -15.39 | -18.33 | -10.45 | 5.22 | -5.59 | -11.39 | -15.30 | -8.46 | 6.67 | -9.13 | -18.20 | -23.39 | -12.05 |
Mitochondria | -0.97 | -2.17 | -2.28 | -0.32 | -1.41 | -2.83 | -8.09 | -2.39 | -2.23 | -2.23 | -1.61 | -5.52 | -2.69 | -2.25 | -2.42 |
Mitotic Spindle | 1.12 | -4.46 | -1.56 | -2.93 | 4.14 | -4.47 | 2.30 | -1.94 | -1.18 | -2.11 | -3.14 | -3.30 | -2.15 | -1.48 | -0.94 |
None | -1.54 | 4.07 | 2.49 | 3.50 | -1.56 | 0.41 | 3.71 | 2.79 | 2.55 | -1.14 | -1.09 | 5.38 | 3.67 | 4.27 | -1.88 |
Nuclear Periphery | 6.09 | -6.99 | -2.45 | -9.82 | 6.59 | 4.46 | 1.57 | -0.98 | -6.41 | -2.96 | 7.52 | -5.67 | -2.79 | -11.70 | 4.67 |
Nuclear Periphery Foci | 2.95 | -2.99 | 1.94 | -1.00 | 5.09 | 2.53 | 9.29 | 6.75 | 4.43 | -3.84 | 3.91 | 6.26 | 5.19 | 1.57 | -0.83 |
Nucleolus | 1.01 | -1.69 | 0.81 | -0.56 | 5.11 | -1.89 | -2.49 | -1.75 | -1.16 | -1.37 | 0.20 | -0.79 | -1.11 | -1.30 | -0.79 |
Nucleus | 3.14 | -4.73 | 4.15 | 1.61 | 6.47 | -2.57 | 7.19 | 2.53 | 4.45 | -3.85 | -0.10 | 1.52 | 4.70 | 4.40 | 2.41 |
Peroxisomes | -1.36 | -6.27 | -5.08 | -2.60 | -4.43 | -1.20 | -7.95 | -2.06 | -2.02 | 0.70 | -1.55 | -8.08 | -3.00 | -2.65 | 1.25 |
Vacuole | 3.06 | -31.03 | -37.93 | -42.14 | 0.48 | -0.89 | -29.80 | -41.37 | -41.32 | -17.58 | 1.42 | -42.57 | -55.40 | -58.15 | -14.28 |
Vacuole Periphery | 5.05 | -1.45 | 5.78 | 0.82 | 7.87 | 3.54 | 2.91 | 5.43 | 2.16 | 3.96 | 6.05 | 3.60 | 7.65 | 1.84 | 5.55 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|