Standard name | |
---|---|
Human Ortholog | |
Description | Dolichyl pyrophosphate (Dol-P-P) phosphatase; has a luminally oriented active site in the ER; cleaves the anhydride linkage in Dol-P-P; required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 6 | 8 | 8 | 1 | 5 | 10 | 15 | – | 9 | 11 | 8 | 9 | 9 | 15 | 4 | 1 | 5 | 10 | 2 | 3 | 2 | 5 | 7 | 4 |
Bud | 2 | 1 | 2 | 0 | 1 | 13 | 6 | – | 8 | 6 | 6 | 10 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 2 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 6 | 3 | 2 | 5 | 2 | 6 | – | 7 | 7 | 13 | 14 | 27 | 30 | 45 | 5 | 26 | 7 | 0 | 0 | 0 | 2 | 1 | 0 |
Cytoplasm | 43 | 59 | 50 | 51 | 116 | 89 | 137 | – | 94 | 102 | 95 | 92 | 64 | 93 | 79 | 9 | 31 | 19 | 3 | 18 | 7 | 11 | 10 | 13 |
Endoplasmic Reticulum | 80 | 81 | 60 | 23 | 32 | 32 | 39 | – | 33 | 43 | 38 | 45 | 193 | 209 | 186 | 0 | 0 | 1 | 148 | 154 | 144 | 204 | 283 | 239 |
Endosome | 12 | 1 | 2 | 4 | 7 | 0 | 5 | – | 2 | 7 | 4 | 1 | 2 | 2 | 2 | 0 | 0 | 2 | 1 | 5 | 0 | 2 | 2 | 4 |
Golgi | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 6 | 0 | 1 | 0 | 3 | 2 |
Mitochondria | 2 | 3 | 1 | 1 | 0 | 14 | 6 | – | 13 | 25 | 37 | 35 | 1 | 4 | 0 | 0 | 2 | 2 | 5 | 10 | 8 | 10 | 8 | 7 |
Nucleus | 0 | 1 | 0 | 0 | 1 | 2 | 1 | – | 1 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 37 | 12 | 11 | 4 | 18 | 9 | 34 | – | 11 | 24 | 16 | 12 | 3 | 7 | 4 | 3 | 11 | 10 | 7 | 16 | 3 | 10 | 11 | 24 |
Unique Cell Count | 160 | 161 | 124 | 79 | 168 | 146 | 220 | 160 | 199 | 190 | 197 | 268 | 315 | 279 | 17 | 71 | 45 | 180 | 215 | 174 | 256 | 339 | 304 | |
Labelled Cell Count | 187 | 173 | 137 | 86 | 185 | 172 | 251 | 180 | 230 | 222 | 223 | 301 | 363 | 321 | 18 | 78 | 52 | 180 | 215 | 174 | 256 | 339 | 304 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.0 | 7.3 | 7.9 | 7.4 | 8.5 | 5.4 | 7.1 | – | 6.5 | 6.5 | 6.4 | 6.4 | 8.6 | 8.3 | 9.0 | 10.7 | 11.5 | 10.8 | 7.6 | 7.9 | 8.1 |
Std Deviation (1e-4) | 1.2 | 1.0 | 1.2 | 0.9 | 1.1 | 1.5 | 1.1 | – | 1.3 | 1.3 | 0.9 | 1.0 | 1.0 | 1.4 | 1.2 | 1.5 | 1.6 | 1.9 | 1.3 | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.1 | -0.54 | -0.16 | – | -0.29 | -0.27 | -0.31 | -0.3 | 0.12 | 0.07 | 0.18 | 0.44 | 0.54 | 0.45 | -0.06 | -0.0 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | -1.4 | 0.1 | 0.1 | – | -0.3 | -0.3 | -0.9 | -0.7 | -1.4 | -0.7 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 2.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 1.7 | 1.8 | 2.7 | 2.5 | 3.9 | 0 | 6.5 | 0 |
Cytoplasm | 3.4 | 4.9 | 3.4 | 3.9 | – | 3.1 | 1.9 | 1.7 | 1.1 | -3.3 | -2.2 | -2.4 | 1.0 | 0.5 | 0.2 |
Endoplasmic Reticulum | -2.7 | -5.3 | -4.6 | -6.0 | – | -4.9 | -5.0 | -5.3 | -4.7 | 4.5 | 3.5 | 3.5 | -3.8 | -7.0 | -5.5 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.1 | 0 | – | 2.8 | 3.8 | 5.0 | 4.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.0 | 0.5 | -0.8 | 1.7 | – | -0.6 | 0.9 | -0.1 | -0.9 | 0 | -3.2 | 0 | 0 | 1.4 | 2.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.1645 | 3.9856 | 4.0326 | 3.3646 | 3.3661 | 3.2324 | 4.5935 | 4.8746 | 4.8924 | 4.7747 | 4.0461 | 4.5883 | 7.3362 | 8.9982 | 8.2075 | 8.4585 | 7.8976 | 8.4293 |
Actin | 0.0791 | 0.0039 | 0.0147 | 0.0008 | 0.0004 | 0.003 | 0.0642 | 0.0048 | 0.0295 | 0.0032 | 0.0058 | 0.0008 | 0.0133 | 0.0006 | 0.0027 | 0.0047 | 0.0021 | 0.0011 |
Bud | 0.0016 | 0.0006 | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0009 | 0.0097 | 0.0042 | 0.0004 | 0.0003 | 0.0009 | 0.0001 | 0.0051 | 0.0001 | 0 | 0.0003 | 0.0008 |
Bud Neck | 0.0079 | 0.0003 | 0.0011 | 0.0002 | 0.0004 | 0.001 | 0.0061 | 0.0008 | 0.002 | 0.0008 | 0.0719 | 0.0009 | 0.001 | 0.0001 | 0.0004 | 0.0005 | 0.0004 | 0.0009 |
Bud Periphery | 0.0034 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0006 | 0.0011 | 0.002 | 0.0018 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.002 | 0.0001 | 0.0001 | 0.0006 | 0.0005 |
Bud Site | 0.0108 | 0.0083 | 0.0172 | 0.0001 | 0.0002 | 0.0004 | 0.006 | 0.0045 | 0.0055 | 0.0006 | 0.0086 | 0.0001 | 0.0011 | 0.0118 | 0.0005 | 0.0002 | 0.0006 | 0.0001 |
Cell Periphery | 0.0025 | 0.0007 | 0.0017 | 0.0005 | 0.0002 | 0.0002 | 0.0009 | 0.0008 | 0.0012 | 0.0003 | 0.0019 | 0.0002 | 0.0017 | 0.0015 | 0.0019 | 0.0017 | 0.0004 | 0.0006 |
Cytoplasm | 0.0723 | 0.2826 | 0.299 | 0.2094 | 0.3965 | 0.1878 | 0.0778 | 0.2719 | 0.3103 | 0.3384 | 0.2191 | 0.2431 | 0.1054 | 0.2459 | 0.205 | 0.1552 | 0.143 | 0.1897 |
Cytoplasmic Foci | 0.1286 | 0.0118 | 0.0192 | 0.0061 | 0.0127 | 0.0124 | 0.0395 | 0.0185 | 0.0206 | 0.0256 | 0.0448 | 0.0045 | 0.0029 | 0.0066 | 0.0094 | 0.0074 | 0.0081 | 0.0075 |
Eisosomes | 0.0009 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0005 | 0 | 0.0002 | 0 | 0 | 0 | 0.0017 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.2378 | 0.5395 | 0.4971 | 0.6333 | 0.3039 | 0.5534 | 0.3804 | 0.5244 | 0.4531 | 0.3433 | 0.531 | 0.665 | 0.8112 | 0.6559 | 0.6859 | 0.787 | 0.6788 | 0.7241 |
Endosome | 0.13 | 0.0653 | 0.0641 | 0.0921 | 0.1913 | 0.0804 | 0.0883 | 0.0561 | 0.0661 | 0.1769 | 0.077 | 0.0181 | 0.0099 | 0.0235 | 0.0294 | 0.0137 | 0.0798 | 0.0256 |
Golgi | 0.0516 | 0.0051 | 0.016 | 0.0049 | 0.023 | 0.0207 | 0.0372 | 0.0058 | 0.0145 | 0.0623 | 0.0048 | 0.0004 | 0.0015 | 0.0016 | 0.0046 | 0.0024 | 0.0099 | 0.0013 |
Lipid Particles | 0.0534 | 0.0078 | 0.0122 | 0.0052 | 0.0037 | 0.0279 | 0.0614 | 0.0058 | 0.0077 | 0.0042 | 0.0053 | 0.0005 | 0.0096 | 0.0168 | 0.0278 | 0.0135 | 0.0134 | 0.0102 |
Mitochondria | 0.0275 | 0.0032 | 0.0116 | 0.001 | 0.0091 | 0.0293 | 0.0413 | 0.0022 | 0.0028 | 0.0067 | 0.0005 | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0.0145 | 0.0004 |
None | 0.0579 | 0.0127 | 0.0167 | 0.0016 | 0.0143 | 0.0014 | 0.0147 | 0.0201 | 0.0411 | 0.0028 | 0.0024 | 0.0427 | 0.0232 | 0.0069 | 0.0132 | 0.0008 | 0.0046 | 0.0104 |
Nuclear Periphery | 0.0236 | 0.029 | 0.0121 | 0.0259 | 0.0121 | 0.0446 | 0.0669 | 0.0267 | 0.011 | 0.0137 | 0.0125 | 0.0091 | 0.01 | 0.0075 | 0.009 | 0.0074 | 0.0175 | 0.0136 |
Nucleolus | 0.0025 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.006 | 0.0023 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0005 | 0 |
Nucleus | 0.0098 | 0.004 | 0.0015 | 0.0021 | 0.0033 | 0.0048 | 0.0467 | 0.0148 | 0.0044 | 0.0023 | 0.0019 | 0.0038 | 0.0011 | 0.0012 | 0.0011 | 0.0007 | 0.0021 | 0.0015 |
Peroxisomes | 0.058 | 0.0002 | 0.0039 | 0 | 0.0008 | 0.0055 | 0.0161 | 0.0008 | 0.0051 | 0.001 | 0.001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0033 | 0.0001 |
Punctate Nuclear | 0.0226 | 0.0019 | 0.0005 | 0.0003 | 0.0008 | 0.0021 | 0.0271 | 0.008 | 0.0024 | 0.0004 | 0.0011 | 0.0034 | 0.0005 | 0.0006 | 0.0004 | 0.0004 | 0.0026 | 0.0005 |
Vacuole | 0.0142 | 0.0205 | 0.0083 | 0.0137 | 0.0192 | 0.0194 | 0.0127 | 0.0179 | 0.0148 | 0.0129 | 0.0082 | 0.0048 | 0.0047 | 0.0112 | 0.0072 | 0.0037 | 0.0139 | 0.0101 |
Vacuole Periphery | 0.0039 | 0.002 | 0.0017 | 0.0027 | 0.0076 | 0.0045 | 0.0041 | 0.0021 | 0.0015 | 0.0037 | 0.0013 | 0.0008 | 0.0003 | 0.0005 | 0.0006 | 0.0003 | 0.0037 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.9613 | 8.2198 | 5.0829 | 14.8008 | 9.9348 | 10.7216 | 8.6573 | 14.4909 | 10.0042 | 16.9469 |
Translational Efficiency | 1.5364 | 1.3365 | 2.7805 | 0.4494 | 1.1255 | 1.5002 | 2.1132 | 0.9704 | 0.993 | 0.7576 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1317 | 731 | 67 | 1503 | 1434 | 1823 | 1171 | 1168 | 2751 | 2554 | 1238 | 2671 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 841.04 | 959.90 | 1038.23 | 1106.38 | 828.25 | 960.30 | 966.83 | 1080.43 | 834.37 | 960.19 | 970.69 | 1095.03 |
Standard Deviation | 113.30 | 134.90 | 130.50 | 164.26 | 98.90 | 126.73 | 123.27 | 181.66 | 106.23 | 129.12 | 124.72 | 172.57 |
Intensity Change Log 2 | — | 0.190710 | 0.303880 | 0.395601 | — | 0.213419 | 0.223196 | 0.383467 | — | 0.202022 | 0.264411 | 0.389593 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001350 | 0.002018 | 0.002986 | 0.004146 | 0.000319 | 0.001459 | 0.003420 | 0.006064 | 0.000812 | 0.001619 | 0.003397 | 0.004985 |
Bud Neck | 0.004197 | 0.002870 | 0.002198 | 0.008427 | 0.001658 | 0.004674 | 0.003371 | 0.012431 | 0.002874 | 0.004158 | 0.003308 | 0.010178 |
Bud Site | 0.012037 | 0.009635 | 0.006145 | 0.024017 | 0.001832 | 0.009661 | 0.008165 | 0.045132 | 0.006718 | 0.009653 | 0.008055 | 0.033250 |
Cell Periphery | 0.004275 | 0.002807 | 0.001299 | 0.001703 | 0.000796 | 0.001547 | 0.001518 | 0.001906 | 0.002461 | 0.001908 | 0.001507 | 0.001792 |
Cytoplasm | 0.202465* | 0.138264* | 0.170711* | 0.153466* | 0.176499* | 0.171440* | 0.170074* | 0.169115* | 0.188930* | 0.161944* | 0.170108* | 0.160309* |
Cytoplasmic Foci | 0.040514 | 0.080642 | 0.057487 | 0.042680 | 0.035559 | 0.047664 | 0.027873 | 0.073157 | 0.037931 | 0.057103 | 0.029476 | 0.056008 |
Eisosomes | 0.002906 | 0.000715 | 0.000622 | 0.000349 | 0.000722 | 0.000926 | 0.000860 | 0.000569 | 0.001767 | 0.000866 | 0.000847 | 0.000445 |
Endoplasmic Reticulum | 0.577700* | 0.600848* | 0.347763* | 0.414921* | 0.637449* | 0.610066* | 0.598224* | 0.327231* | 0.608845* | 0.607428* | 0.584670* | 0.376575* |
Endosome | 0.010027 | 0.021503 | 0.024304 | 0.035070 | 0.009225 | 0.016570 | 0.010283 | 0.041632 | 0.009609 | 0.017982 | 0.011042 | 0.037939 |
Golgi | 0.037910 | 0.035195 | 0.002559 | 0.008442 | 0.019772 | 0.022644 | 0.004555 | 0.020765 | 0.028455 | 0.026236 | 0.004447 | 0.013831 |
Lipid Particles | 0.047775 | 0.043856 | 0.043450 | 0.059784 | 0.057633 | 0.044051 | 0.031639 | 0.072210 | 0.052913 | 0.043995 | 0.032279 | 0.065217 |
Mitochondria | 0.010743 | 0.006082 | 0.000208 | 0.001813 | 0.006678 | 0.006745 | 0.000649 | 0.003486 | 0.008624 | 0.006555 | 0.000625 | 0.002545 |
Mitotic Spindle | 0.000390 | 0.000409 | 0.000441 | 0.014051 | 0.000126 | 0.001411 | 0.000374 | 0.013634 | 0.000253 | 0.001124 | 0.000377 | 0.013868 |
None | 0.010647 | 0.001369 | 0.000333 | 0.001768 | 0.004978 | 0.005235 | 0.001677 | 0.003081 | 0.007692 | 0.004129 | 0.001605 | 0.002342 |
Nuclear Periphery | 0.000775 | 0.000657 | 0.021723 | 0.005029 | 0.002807 | 0.001262 | 0.004351 | 0.002604 | 0.001834 | 0.001089 | 0.005291 | 0.003968 |
Nuclear Periphery Foci | 0.004191 | 0.009109 | 0.120071 | 0.045979 | 0.006025 | 0.007385 | 0.041457 | 0.030085 | 0.005147 | 0.007878 | 0.045712 | 0.039029 |
Nucleolus | 0.000397 | 0.000172 | 0.000222 | 0.000343 | 0.000289 | 0.000506 | 0.000206 | 0.000523 | 0.000341 | 0.000411 | 0.000207 | 0.000422 |
Nucleus | 0.010380 | 0.005529 | 0.034591 | 0.018773 | 0.016307 | 0.015449 | 0.035213 | 0.016504 | 0.013470 | 0.012610 | 0.035180 | 0.017781 |
Peroxisomes | 0.009057 | 0.004048 | 0.001548 | 0.001475 | 0.002518 | 0.003309 | 0.000897 | 0.002872 | 0.005648 | 0.003520 | 0.000933 | 0.002086 |
Vacuole | 0.008743 | 0.028760 | 0.160789 | 0.154869 | 0.016967 | 0.024683 | 0.054520 | 0.153431 | 0.013030 | 0.025850 | 0.060271 | 0.154240 |
Vacuole Periphery | 0.003521 | 0.005511 | 0.000551 | 0.002896 | 0.001842 | 0.003312 | 0.000671 | 0.003569 | 0.002646 | 0.003941 | 0.000665 | 0.003190 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.83 | -1.49 | -4.97 | -3.50 | -0.61 | -4.14 | -3.91 | -6.53 | -5.07 | -2.29 | -3.46 | -3.45 | -8.42 | -6.40 | -1.76 |
Bud Neck | 1.59 | 1.91 | -4.77 | -7.57 | -6.90 | -4.45 | -3.61 | -8.83 | -6.03 | -7.32 | -2.20 | -1.06 | -10.20 | -8.20 | -9.63 |
Bud Site | 1.05 | 1.98 | -4.72 | -5.94 | -6.27 | -6.03 | -5.35 | -11.45 | -8.90 | -9.20 | -2.22 | -1.21 | -12.26 | -10.63 | -10.78 |
Cell Periphery | 1.91 | 4.47 | 4.57 | 2.03 | -0.68 | -4.48 | -3.60 | -4.04 | -0.98 | -0.96 | 1.70 | 2.93 | 2.43 | 0.78 | -0.79 |
Cytoplasm | 7.61 | 1.07 | 5.40 | -2.74 | 0.37 | 0.74 | 0.60 | 0.44 | -0.19 | -0.12 | 5.11 | 2.51 | 4.12 | -0.77 | 0.82 |
Cytoplasmic Foci | -7.34 | -1.68 | -1.11 | 6.85 | 1.37 | -3.93 | 2.68 | -10.14 | -6.51 | -12.56 | -7.12 | 3.38 | -7.95 | 0.12 | -10.64 |
Eisosomes | 4.94 | 4.20 | 5.90 | 3.64 | 0.85 | -1.32 | -0.56 | 1.31 | 2.23 | 1.21 | 3.85 | 3.02 | 6.12 | 3.92 | 1.84 |
Endoplasmic Reticulum | -1.42 | 6.22 | 14.62 | 13.64 | -1.16 | 2.38 | 3.56 | 24.58 | 22.86 | 19.72 | 0.20 | 2.71 | 27.34 | 26.17 | 19.00 |
Endosome | -6.74 | -5.21 | -15.57 | -6.57 | -3.54 | -6.75 | -1.46 | -17.70 | -12.37 | -16.92 | -8.89 | -2.01 | -23.77 | -14.49 | -21.88 |
Golgi | 0.91 | 17.65 | 14.74 | 10.83 | -4.53 | -1.77 | 12.79 | 0.08 | 1.26 | -7.02 | 1.43 | 20.43 | 10.45 | 8.70 | -7.37 |
Lipid Particles | 1.02 | 0.63 | -3.48 | -4.02 | -2.34 | 3.86 | 7.62 | -3.03 | -6.43 | -9.45 | 3.52 | 7.93 | -4.25 | -7.53 | -11.41 |
Mitochondria | 3.48 | 12.37 | 10.14 | 4.09 | -5.44 | -0.07 | 7.73 | 3.59 | 4.46 | -5.01 | 2.71 | 13.78 | 9.75 | 7.29 | -6.20 |
Mitotic Spindle | -0.06 | -0.30 | -6.77 | -6.77 | -6.66 | -4.37 | -2.04 | -7.00 | -6.31 | -6.87 | -3.92 | -1.10 | -9.66 | -8.98 | -9.55 |
None | 6.11 | 6.92 | 5.73 | -0.59 | -2.73 | -0.21 | 2.86 | 1.46 | 1.75 | -0.98 | 3.43 | 5.65 | 5.37 | 2.39 | -0.76 |
Nuclear Periphery | 0.61 | -4.80 | -7.95 | -8.40 | 3.76 | 3.23 | -2.52 | 0.36 | -3.27 | 3.16 | 2.74 | -6.58 | -5.12 | -8.26 | 2.23 |
Nuclear Periphery Foci | -2.52 | -8.95 | -17.24 | -12.33 | 5.49 | -1.39 | -15.10 | -10.47 | -9.73 | 3.05 | -3.15 | -17.29 | -19.87 | -17.17 | 1.93 |
Nucleolus | 2.61 | 1.67 | 0.05 | -3.10 | -1.87 | -2.54 | 1.03 | -2.57 | -0.23 | -3.44 | -1.01 | 1.83 | -1.56 | -0.60 | -3.37 |
Nucleus | 5.93 | -3.64 | -6.74 | -10.27 | 2.21 | 0.59 | -8.14 | -0.70 | -1.33 | 7.63 | 0.91 | -10.26 | -5.18 | -5.86 | 7.37 |
Peroxisomes | 5.63 | 9.26 | 10.48 | 4.57 | 0.29 | -2.15 | 6.87 | -0.71 | 1.14 | -5.26 | 4.59 | 12.70 | 8.89 | 4.54 | -5.53 |
Vacuole | -7.27 | -7.57 | -29.74 | -23.80 | -0.37 | -3.85 | -12.37 | -23.99 | -22.32 | -15.92 | -8.19 | -15.47 | -37.95 | -33.45 | -20.85 |
Vacuole Periphery | -1.85 | 4.08 | 0.97 | 3.08 | -4.50 | -4.37 | 3.41 | -3.58 | -0.21 | -5.48 | -2.74 | 4.35 | -0.74 | 2.13 | -5.51 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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