Standard name | |
---|---|
Human Ortholog | |
Description | Protein that mediates sphingolipid homeostasis; evolutionarily conserved, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; ORM1 has a paralog, ORM2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 6 | 6 | 12 | 3 | 6 | 7 | – | 17 | 16 | 25 | 23 | 5 | 11 | 3 | 3 | 2 | 8 | 6 | 4 | 2 | 1 | 6 | 6 |
Bud | 0 | 1 | 0 | 1 | 0 | 3 | 2 | – | 14 | 3 | 13 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 10 | 9 | 27 | 25 | 18 | 16 | 31 | – | 24 | 32 | 45 | 45 | 33 | 89 | 95 | 16 | 28 | 16 | 3 | 1 | 2 | 4 | 13 | 11 |
Cytoplasm | 23 | 33 | 31 | 31 | 53 | 69 | 94 | – | 106 | 58 | 70 | 73 | 30 | 46 | 87 | 21 | 17 | 26 | 6 | 9 | 4 | 5 | 10 | 11 |
Endoplasmic Reticulum | 203 | 204 | 245 | 105 | 118 | 88 | 119 | – | 145 | 175 | 136 | 168 | 242 | 302 | 317 | 1 | 0 | 2 | 235 | 222 | 300 | 194 | 460 | 441 |
Endosome | 0 | 0 | 2 | 1 | 1 | 1 | 9 | – | 2 | 1 | 4 | 3 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 |
Golgi | 2 | 0 | 12 | 4 | 10 | 0 | 4 | – | 3 | 1 | 1 | 1 | 2 | 5 | 2 | 6 | 14 | 13 | 2 | 0 | 3 | 0 | 2 | 3 |
Mitochondria | 7 | 1 | 0 | 18 | 2 | 43 | 9 | – | 51 | 53 | 57 | 68 | 1 | 4 | 0 | 2 | 3 | 5 | 5 | 9 | 4 | 2 | 6 | 7 |
Nucleus | 2 | 0 | 0 | 1 | 2 | 3 | 5 | – | 6 | 6 | 1 | 3 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 11 | 5 | 7 | 3 | 4 | 2 | 7 | – | 15 | 6 | 12 | 23 | 7 | 6 | 9 | 2 | 1 | 0 | 1 | 3 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 30 | 13 | 52 | 137 | 119 | 69 | 241 | – | 159 | 93 | 106 | 85 | 3 | 23 | 53 | 21 | 13 | 26 | 5 | 5 | 2 | 2 | 10 | 13 |
Unique Cell Count | 249 | 245 | 303 | 269 | 274 | 250 | 453 | 442 | 379 | 375 | 395 | 274 | 390 | 455 | 66 | 68 | 73 | 270 | 263 | 326 | 213 | 521 | 503 | |
Labelled Cell Count | 291 | 272 | 383 | 341 | 330 | 300 | 528 | 543 | 445 | 470 | 501 | 325 | 487 | 568 | 73 | 78 | 97 | 270 | 263 | 326 | 213 | 521 | 503 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.1 | 10.4 | 12.4 | 11.8 | 12.7 | 9.8 | 10.6 | – | 9.8 | 9.5 | 9.8 | 9.4 | 12.0 | 12.3 | 12.6 | 15.5 | 16.7 | 14.3 | 10.8 | 11.2 | 11.7 |
Std Deviation (1e-4) | 2.5 | 2.3 | 2.5 | 2.4 | 2.3 | 6.2 | 1.8 | – | 1.8 | 1.8 | 1.8 | 1.6 | 2.3 | 2.4 | 2.5 | 3.4 | 4.0 | 4.3 | 2.5 | 2.4 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.04 | -0.34 | -0.23 | – | -0.33 | -0.38 | -0.34 | -0.39 | -0.05 | -0.01 | 0.03 | 0.33 | 0.43 | 0.2 | -0.2 | -0.15 | -0.08 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.7 | 0 | 0.3 | -0.5 | – | 1.4 | 1.6 | 2.9 | 2.5 | -0.1 | 0.7 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 3.1 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.2 | -1.0 | -1.1 | -1.0 | – | -1.8 | -0.2 | 1.3 | 1.1 | 1.2 | 4.9 | 4.4 | 3.5 | 6.8 | 3.1 |
Cytoplasm | 0.5 | 3.1 | 5.3 | 3.8 | – | 4.8 | 2.0 | 3.1 | 3.0 | 0.3 | 0.6 | 3.3 | 4.6 | 3.3 | 5.4 |
Endoplasmic Reticulum | -10.2 | -9.4 | -10.9 | -14.7 | – | -12.9 | -9.3 | -11.6 | -10.2 | 2.5 | -1.1 | -3.4 | -12.4 | -12.7 | -12.6 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -1.8 | -0.2 | -3.2 | -2.9 | – | -3.1 | -3.5 | -3.5 | -3.6 | -2.5 | -2.3 | -3.5 | 0 | 0 | 0 |
Mitochondria | 4.6 | 0 | 7.5 | 0 | – | 6.1 | 6.8 | 7.1 | 7.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | -0.7 | 0 | -0.8 | – | 0.9 | -0.7 | 0.7 | 2.3 | 0.2 | -0.7 | -0.3 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 8.6 | 6.9 | 3.0 | 10.0 | – | 5.6 | 2.3 | 3.4 | 1.4 | -6.6 | -4.7 | -2.2 | 2.7 | 0.4 | 3.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.6017 | 6.1596 | 5.1954 | 5.1004 | 4.9386 | 5.5987 | 7.847 | 7.7753 | 7.4464 | 7.4819 | 6.8246 | 8.4203 | 11.2121 | 11.0379 | 11.2188 | 11.7094 | 11.0731 | 11.0106 |
Actin | 0.027 | 0.0051 | 0.0064 | 0.0278 | 0.006 | 0.0015 | 0.0339 | 0.0045 | 0.023 | 0.0021 | 0.0443 | 0.0045 | 0.02 | 0.0011 | 0.0002 | 0.0047 | 0.0007 | 0.0009 |
Bud | 0.0042 | 0.0042 | 0.0051 | 0.0003 | 0.0085 | 0.0025 | 0.0006 | 0.0004 | 0.0004 | 0.0017 | 0.0008 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Bud Neck | 0.0022 | 0.0009 | 0.0008 | 0.0004 | 0.0011 | 0.0028 | 0.0109 | 0.0007 | 0.0024 | 0.0008 | 0.001 | 0.0086 | 0.0005 | 0.0005 | 0.0001 | 0.0009 | 0.0002 | 0.0014 |
Bud Periphery | 0.0023 | 0.0025 | 0.0034 | 0.0003 | 0.0022 | 0.0017 | 0.0011 | 0.0004 | 0.0008 | 0.0016 | 0.0011 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Bud Site | 0.0062 | 0.0459 | 0.0158 | 0.001 | 0.0028 | 0.0009 | 0.007 | 0.0086 | 0.007 | 0.0124 | 0.0046 | 0.0007 | 0.0004 | 0.0008 | 0.0001 | 0.0001 | 0 | 0.0002 |
Cell Periphery | 0.0026 | 0.0029 | 0.0035 | 0.0006 | 0.001 | 0.0009 | 0.0018 | 0.0036 | 0.0042 | 0.0017 | 0.0007 | 0.0011 | 0.0012 | 0.0022 | 0.0008 | 0.0013 | 0.0005 | 0.0006 |
Cytoplasm | 0.0598 | 0.1094 | 0.0698 | 0.02 | 0.048 | 0.0946 | 0.0759 | 0.1505 | 0.0867 | 0.1445 | 0.0572 | 0.0857 | 0.0269 | 0.1117 | 0.0209 | 0.1509 | 0.0343 | 0.0456 |
Cytoplasmic Foci | 0.0174 | 0.0062 | 0.0071 | 0.0072 | 0.0117 | 0.0044 | 0.0244 | 0.0064 | 0.0043 | 0.0286 | 0.0121 | 0.008 | 0.0056 | 0.0021 | 0.001 | 0.0023 | 0.0016 | 0.0008 |
Eisosomes | 0.0009 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0005 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.7102 | 0.7125 | 0.7418 | 0.8967 | 0.7697 | 0.8016 | 0.668 | 0.7507 | 0.7806 | 0.7069 | 0.7005 | 0.8227 | 0.907 | 0.8533 | 0.9579 | 0.8207 | 0.9448 | 0.9291 |
Endosome | 0.0177 | 0.0104 | 0.0155 | 0.0054 | 0.0401 | 0.0083 | 0.0333 | 0.0119 | 0.0184 | 0.0166 | 0.0391 | 0.0076 | 0.0037 | 0.0047 | 0.0046 | 0.0036 | 0.007 | 0.0017 |
Golgi | 0.0057 | 0.0019 | 0.0029 | 0.0018 | 0.0176 | 0.0004 | 0.0096 | 0.004 | 0.0091 | 0.0163 | 0.013 | 0.0023 | 0.0015 | 0.0005 | 0.0007 | 0.0008 | 0.0011 | 0.0002 |
Lipid Particles | 0.0103 | 0.0045 | 0.013 | 0.0026 | 0.0147 | 0.002 | 0.0239 | 0.0126 | 0.0179 | 0.0059 | 0.0293 | 0.0037 | 0.0031 | 0.0037 | 0.0023 | 0.0017 | 0.0016 | 0.0014 |
Mitochondria | 0.0051 | 0.0006 | 0.0105 | 0.0007 | 0.0026 | 0.0005 | 0.0059 | 0.0035 | 0.009 | 0.0046 | 0.0094 | 0.0016 | 0.0002 | 0.0001 | 0.0028 | 0.0001 | 0.0001 | 0 |
None | 0.0215 | 0.0117 | 0.0185 | 0.0069 | 0.0034 | 0.0147 | 0.0033 | 0.0059 | 0.0053 | 0.0071 | 0.0011 | 0.0118 | 0.0033 | 0.0021 | 0.0009 | 0.0008 | 0.0004 | 0.0069 |
Nuclear Periphery | 0.0533 | 0.0393 | 0.0397 | 0.0162 | 0.0299 | 0.0303 | 0.0282 | 0.0183 | 0.009 | 0.0212 | 0.0254 | 0.0169 | 0.0128 | 0.0106 | 0.0043 | 0.0074 | 0.0051 | 0.0065 |
Nucleolus | 0.0061 | 0.0015 | 0.0016 | 0.0004 | 0.0018 | 0.0012 | 0.0118 | 0.0007 | 0.001 | 0.0001 | 0.0029 | 0.0003 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0193 | 0.0219 | 0.0185 | 0.002 | 0.0201 | 0.0177 | 0.0231 | 0.0057 | 0.0093 | 0.0069 | 0.0363 | 0.0165 | 0.0016 | 0.0016 | 0.0005 | 0.002 | 0.0006 | 0.0016 |
Peroxisomes | 0.0012 | 0.0002 | 0.0071 | 0.0005 | 0.0009 | 0.0001 | 0.0125 | 0.0004 | 0.002 | 0.0073 | 0.0082 | 0.0016 | 0.0052 | 0 | 0.0001 | 0 | 0 | 0 |
Punctate Nuclear | 0.0178 | 0.0106 | 0.0084 | 0.0054 | 0.0077 | 0.0084 | 0.0146 | 0.0024 | 0.0004 | 0.0005 | 0.0014 | 0.0022 | 0.0043 | 0.0005 | 0.0001 | 0.0005 | 0.0002 | 0.0001 |
Vacuole | 0.007 | 0.0068 | 0.009 | 0.003 | 0.0086 | 0.0046 | 0.0065 | 0.0081 | 0.0083 | 0.0123 | 0.0093 | 0.0031 | 0.0019 | 0.0041 | 0.0021 | 0.002 | 0.0018 | 0.0027 |
Vacuole Periphery | 0.0023 | 0.0008 | 0.0014 | 0.0007 | 0.0014 | 0.0008 | 0.0016 | 0.0006 | 0.0011 | 0.0009 | 0.0022 | 0.0006 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 33.8113 | 28.0137 | 31.7281 | 48.4542 | 52.3916 | 36.2653 | 43.605 | 58.8608 | 46.2186 | 47.2632 |
Translational Efficiency | 2.3659 | 2.6225 | 2.2767 | 1.4535 | 1.3626 | 1.9441 | 1.6612 | 1.3959 | 1.577 | 1.6039 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
91 | 643 | 1967 | 2047 | 2390 | 1712 | 2590 | 1545 | 2481 | 2355 | 4557 | 3592 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1026.78 | 1056.27 | 1287.71 | 1322.39 | 952.26 | 1094.11 | 1311.36 | 1465.43 | 954.99 | 1083.78 | 1301.15 | 1383.91 |
Standard Deviation | 199.59 | 190.01 | 191.25 | 255.86 | 143.05 | 173.21 | 214.27 | 291.82 | 146.18 | 178.75 | 204.99 | 280.98 |
Intensity Change Log 2 | — | 0.040852 | 0.326681 | 0.365021 | — | 0.200330 | 0.461636 | 0.621897 | — | 0.119791 | 0.393195 | 0.494337 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000407 | 0.002200 | 0.001259 | 0.003136 | 0.000521 | 0.002858 | 0.000365 | 0.001916 | 0.000517 | 0.002678 | 0.000751 | 0.002611 |
Bud Neck | 0.001008 | 0.000959 | 0.000705 | 0.003907 | 0.000549 | 0.001738 | 0.001077 | 0.003791 | 0.000566 | 0.001525 | 0.000917 | 0.003857 |
Bud Site | 0.014940 | 0.010466 | 0.003482 | 0.031226 | 0.003228 | 0.012402 | 0.003984 | 0.012833 | 0.003657 | 0.011873 | 0.003767 | 0.023315 |
Cell Periphery | 0.014525 | 0.003868 | 0.001193 | 0.004978 | 0.003755 | 0.003763 | 0.004788 | 0.008998 | 0.004150 | 0.003791 | 0.003236 | 0.006707 |
Cytoplasm | 0.008902 | 0.037055 | 0.018445 | 0.056800 | 0.047196 | 0.054843 | 0.029114 | 0.033215 | 0.045791 | 0.049986 | 0.024509 | 0.046655 |
Cytoplasmic Foci | 0.002703 | 0.021234 | 0.001046 | 0.011917 | 0.004997 | 0.027183 | 0.001824 | 0.003846 | 0.004912 | 0.025559 | 0.001488 | 0.008445 |
Eisosomes | 0.000037 | 0.000347 | 0.000043 | 0.000172 | 0.000379 | 0.000389 | 0.000057 | 0.000218 | 0.000366 | 0.000378 | 0.000051 | 0.000192 |
Endoplasmic Reticulum | 0.901661* | 0.855982* | 0.952654* | 0.774370* | 0.907272* | 0.798549* | 0.934869* | 0.864802* | 0.907066* | 0.814230* | 0.942545* | 0.813267* |
Endosome | 0.002982 | 0.013343 | 0.003429 | 0.017105 | 0.002030 | 0.013112 | 0.003848 | 0.009697 | 0.002065 | 0.013175 | 0.003667 | 0.013919 |
Golgi | 0.000558 | 0.011472 | 0.000330 | 0.007382 | 0.003776 | 0.019513 | 0.000841 | 0.002022 | 0.003658 | 0.017317 | 0.000621 | 0.005077 |
Lipid Particles | 0.002986 | 0.009828 | 0.001571 | 0.017995 | 0.005934 | 0.017876 | 0.004327 | 0.013185 | 0.005826 | 0.015678 | 0.003137 | 0.015926 |
Mitochondria | 0.005368 | 0.004553 | 0.000288 | 0.002108 | 0.002881 | 0.008616 | 0.000784 | 0.000951 | 0.002972 | 0.007506 | 0.000570 | 0.001610 |
Mitotic Spindle | 0.000076 | 0.001160 | 0.000211 | 0.004205 | 0.000062 | 0.002580 | 0.000057 | 0.003348 | 0.000063 | 0.002192 | 0.000124 | 0.003836 |
None | 0.000475 | 0.004682 | 0.001419 | 0.005080 | 0.007708 | 0.010001 | 0.002951 | 0.001867 | 0.007443 | 0.008549 | 0.002290 | 0.003698 |
Nuclear Periphery | 0.000487 | 0.000747 | 0.001446 | 0.003572 | 0.000486 | 0.000784 | 0.000265 | 0.001813 | 0.000486 | 0.000774 | 0.000775 | 0.002815 |
Nuclear Periphery Foci | 0.002771 | 0.008335 | 0.006020 | 0.030803 | 0.002473 | 0.007643 | 0.002863 | 0.017488 | 0.002484 | 0.007832 | 0.004226 | 0.025076 |
Nucleolus | 0.001924 | 0.000065 | 0.000008 | 0.000096 | 0.000075 | 0.000340 | 0.000022 | 0.000087 | 0.000143 | 0.000265 | 0.000016 | 0.000092 |
Nucleus | 0.033421 | 0.000797 | 0.001761 | 0.004020 | 0.002326 | 0.004097 | 0.001026 | 0.005296 | 0.003467 | 0.003196 | 0.001343 | 0.004568 |
Peroxisomes | 0.000630 | 0.001587 | 0.000039 | 0.000397 | 0.002127 | 0.002612 | 0.000258 | 0.000277 | 0.002072 | 0.002333 | 0.000164 | 0.000346 |
Vacuole | 0.003210 | 0.008003 | 0.004508 | 0.018884 | 0.001690 | 0.007793 | 0.006242 | 0.013548 | 0.001746 | 0.007850 | 0.005494 | 0.016589 |
Vacuole Periphery | 0.000930 | 0.003318 | 0.000142 | 0.001847 | 0.000535 | 0.003308 | 0.000436 | 0.000803 | 0.000550 | 0.003311 | 0.000309 | 0.001398 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.29 | -1.65 | -7.45 | -1.29 | -3.23 | -3.14 | 1.00 | -4.42 | 0.88 | -5.07 | -3.68 | -0.98 | -7.98 | -0.21 | -5.99 |
Bud Neck | 0.06 | 0.30 | -3.67 | -5.58 | -6.98 | -3.16 | -1.56 | -5.57 | -3.30 | -4.23 | -3.19 | -1.68 | -9.20 | -5.64 | -7.47 |
Bud Site | 0.47 | 1.20 | -1.78 | -6.49 | -10.45 | -5.76 | -1.10 | -6.30 | -0.83 | -5.79 | -5.90 | -0.38 | -11.63 | -6.16 | -11.91 |
Cell Periphery | 1.17 | 1.46 | 1.05 | -1.29 | -7.38 | -0.01 | -1.67 | -4.78 | -4.55 | -3.82 | 0.51 | 1.49 | -3.44 | -4.26 | -5.96 |
Cytoplasm | -5.45 | -2.59 | -10.38 | -4.71 | -11.79 | -2.17 | 6.34 | 3.40 | 4.91 | -1.84 | -1.38 | 8.73 | -1.10 | 0.34 | -9.88 |
Cytoplasmic Foci | -6.14 | 1.75 | -6.93 | 2.84 | -10.02 | -10.63 | 5.04 | 0.98 | 10.83 | -3.03 | -11.82 | 5.91 | -4.53 | 9.26 | -10.15 |
Eisosomes | -1.96 | -0.43 | -3.41 | 1.07 | -3.29 | -0.08 | 4.36 | 1.85 | 1.63 | -3.74 | -0.10 | 4.44 | 2.26 | 2.16 | -4.87 |
Endoplasmic Reticulum | 1.56 | -1.80 | 4.85 | 7.15 | 23.33 | 12.77 | -5.18 | 6.76 | -5.59 | 10.52 | 12.70 | -7.52 | 16.17 | 1.28 | 24.44 |
Endosome | -5.68 | -0.48 | -11.21 | -2.42 | -15.30 | -11.79 | -6.59 | -10.71 | 2.70 | -7.84 | -13.79 | -6.74 | -20.65 | -1.11 | -17.15 |
Golgi | -5.87 | 1.45 | -7.33 | 1.99 | -7.68 | -8.98 | 6.93 | 3.50 | 10.11 | -4.03 | -9.78 | 7.58 | -2.13 | 8.48 | -8.23 |
Lipid Particles | -2.48 | 0.66 | -5.65 | -4.41 | -11.39 | -7.57 | 1.91 | -5.19 | 2.12 | -6.15 | -7.82 | 4.11 | -9.20 | -0.48 | -12.21 |
Mitochondria | 0.21 | 1.31 | 0.83 | 2.51 | -6.99 | -5.12 | 6.21 | 4.31 | 6.87 | -0.69 | -5.09 | 6.83 | 3.35 | 6.96 | -5.40 |
Mitotic Spindle | -1.77 | -1.29 | -5.87 | -3.03 | -5.65 | -3.86 | -0.07 | -2.95 | -0.74 | -2.95 | -4.25 | -1.42 | -6.09 | -2.28 | -5.97 |
None | -2.51 | -1.77 | -4.60 | -0.18 | -3.36 | -1.26 | 4.21 | 5.43 | 5.08 | 1.42 | -0.72 | 5.11 | 3.44 | 3.66 | -1.95 |
Nuclear Periphery | -0.65 | -2.91 | -5.92 | -5.22 | -4.18 | -2.70 | 3.27 | -5.31 | -4.09 | -6.31 | -2.39 | -2.55 | -8.13 | -6.89 | -6.92 |
Nuclear Periphery Foci | -2.90 | -2.28 | -11.53 | -9.02 | -11.58 | -5.12 | -0.86 | -9.00 | -5.51 | -8.78 | -6.16 | -3.53 | -15.84 | -11.11 | -14.80 |
Nucleolus | 1.34 | 1.38 | 1.32 | -0.96 | -3.00 | -1.78 | 2.35 | -0.35 | 1.66 | -2.12 | -0.98 | 2.29 | 0.80 | 1.55 | -3.58 |
Nucleus | 1.91 | 1.84 | 1.70 | -8.81 | -4.88 | -2.87 | 4.00 | -4.19 | -1.99 | -5.71 | 0.35 | 2.83 | -2.13 | -3.36 | -7.93 |
Peroxisomes | -2.46 | 2.14 | 0.81 | 4.50 | -8.54 | -1.07 | 6.05 | 5.97 | 6.62 | -0.31 | -0.68 | 6.45 | 5.78 | 7.44 | -4.82 |
Vacuole | -2.23 | -1.30 | -8.74 | -5.80 | -9.42 | -7.62 | -7.45 | -9.37 | -4.59 | -5.56 | -8.13 | -8.37 | -15.38 | -8.31 | -10.79 |
Vacuole Periphery | -3.16 | 3.19 | -2.39 | 1.90 | -5.77 | -6.68 | 1.03 | -1.13 | 5.29 | -1.52 | -7.64 | 3.13 | -4.14 | 4.69 | -5.48 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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