Standard name
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.98 0.98 0.98 0.94 0.92 0.91 0.86 0.91 0.75 0.85 0.98 0.99 0.99 0.94 0.94 0.94 0.73 0.81 0.7 0.8 0.84 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.06 0 0 0 0.06 0.15 0.14 0.32 0.16 0.41 0.28 0 0 0 0 0 0 0.16 0.09 0.14 0.07 0.05 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 5 0 3 1
Bud 0 0 0 1 0 0 4 2 4 1 5 6 1 0 0 0 0 1 0 1 3 2 2 2
Bud Neck 0 0 11 4 15 5 8 18 0 8 5 8 0 1 0 1 2 1 1 0 0 0 0 3
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 3 0 1 2 2 2 0 0 1 0 2 1 1 3 4 7 0 0 3 0 1 2
Cytoplasm 193 178 211 176 317 197 276 349 147 192 131 163 154 233 216 207 193 254 142 156 138 103 175 149
Endoplasmic Reticulum 0 1 2 1 2 0 2 0 0 0 1 0 1 3 1 18 13 20 2 0 1 0 1 3
Endosome 0 0 2 0 2 1 0 3 0 0 0 0 1 1 1 3 4 3 1 4 2 0 0 0
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 2 0 0 1 2 1 3 0 0 0 0
Mitochondria 33 11 1 5 2 13 44 55 55 34 72 54 1 0 1 8 9 8 30 16 26 8 10 9
Nucleus 0 0 0 0 0 3 2 4 1 3 0 1 0 1 1 1 1 2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 3 5 1 1 1 2 0 0 0 2 0 4 1 2 1 2 3 4
Vac/Vac Membrane 0 0 0 1 0 1 0 3 0 1 3 0 2 0 0 4 5 5 2 2 1 1 2 4
Unique Cell Count 203 182 216 180 324 209 300 382 171 212 175 192 157 236 219 220 206 270 195 194 198 129 210 186
Labelled Cell Count 226 191 230 188 340 224 342 441 208 243 219 234 162 242 221 247 233 308 195 194 198 129 210 186


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 3.8 4.4 3.9 4.5 3.7 3.2 3.7 3.0 3.5 3.0 3.2 4.6 4.9 4.8 5.2 5.5 5.1 4.4 4.8 5.1
Std Deviation (1e-4) 0.6 0.8 1.8 1.3 2.0 1.9 1.6 1.3 1.4 1.6 1.5 1.0 1.1 1.2 1.7 1.4 2.1 2.1 1.7 2.4 2.3
Intensity Change (Log2) – – – -0.15 0.03 -0.26 -0.45 -0.23 -0.56 -0.32 -0.55 -0.43 0.07 0.18 0.13 0.26 0.34 0.24 0.02 0.15 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2091 0.1674 0.157 -0.0459 0.0713 0.3474 -0.0568 -0.277 -0.3214 -0.2231 -0.2858 -0.3246 -1.3539 -1.2185 -1.4279 -1.2158 -1.6109 -1.3721
Actin 0.0339 0.0005 0.0035 0.0194 0.0408 0.0019 0.0239 0.0057 0.006 0.0301 0.0224 0.0078 0.0026 0.0005 0.0024 0.0102 0.0411 0.0044
Bud 0.001 0.0041 0.0033 0.0002 0.0097 0.0015 0.0018 0.0002 0.0003 0.0008 0.0005 0.0002 0.0004 0.0017 0.0006 0.0003 0.0008 0.0011
Bud Neck 0.0056 0.0001 0.0013 0.0008 0.0016 0.0002 0.0008 0.0002 0.0012 0.0014 0.008 0.0003 0.0002 0.0001 0.0009 0.0003 0.0032 0.0002
Bud Periphery 0.0025 0.0081 0.0007 0.0004 0.0023 0.0011 0.0062 0.0002 0.0004 0.0032 0.0009 0.0003 0.0011 0.0017 0.0011 0.0003 0.0008 0.0079
Bud Site 0.0064 0.0068 0.0077 0.0014 0.0025 0.0001 0.0025 0.0034 0.0035 0.0021 0.0015 0.0001 0.0026 0.0048 0.0101 0.001 0.0012 0.0001
Cell Periphery 0.0003 0.0002 0.0002 0.0001 0.0002 0.0001 0.0004 0.0002 0.0001 0.0001 0.0002 0 0.0011 0.0001 0.0008 0.0001 0.0001 0
Cytoplasm 0.039 0.063 0.028 0.0669 0.04 0.1038 0.0876 0.0569 0.0381 0.0592 0.0629 0.1757 0.1333 0.0734 0.0554 0.0889 0.0212 0.227
Cytoplasmic Foci 0.0142 0.0023 0.0058 0.0081 0.0046 0.0085 0.0324 0.0044 0.0175 0.014 0.0185 0.0095 0.0126 0.0027 0.0198 0.011 0.0589 0.008
Eisosomes 0.0005 0.0001 0.0003 0.0001 0.0003 0.0001 0.0003 0.0003 0.0004 0.0002 0.0002 0.0001 0.0006 0.0004 0.0004 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0012 0.0004 0.0009 0.0001 0.0004 0.0004 0.0027 0.0009 0.0006 0.001 0.0038 0.0017 0.0018 0.0005 0.0006 0.0011 0.0006 0.0009
Endosome 0.0063 0.0008 0.0063 0.0003 0.0014 0.0029 0.028 0.0024 0.0076 0.0368 0.0416 0.0042 0.008 0.0003 0.0072 0.0257 0.0115 0.0035
Golgi 0.005 0.0001 0.0034 0.0006 0.0022 0.0002 0.0109 0.0006 0.0039 0.0093 0.013 0.0031 0.0021 0.0001 0.0028 0.0125 0.012 0.001
Lipid Particles 0.0178 0.0001 0.0051 0.0006 0.004 0.0005 0.0187 0.0009 0.0039 0.0053 0.007 0.0116 0.0046 0.0002 0.0074 0.0119 0.0024 0.0025
Mitochondria 0.0116 0.0004 0.0042 0.0018 0.0032 0.0006 0.0307 0.001 0.0242 0.0054 0.0269 0.0205 0.0086 0.0003 0.0112 0.0041 0.003 0.0039
None 0.8295 0.9107 0.9151 0.885 0.857 0.872 0.6839 0.9172 0.8766 0.8151 0.7621 0.7553 0.8047 0.9102 0.8676 0.8243 0.8082 0.7347
Nuclear Periphery 0.0025 0.0002 0.0006 0 0.0007 0.0003 0.0087 0.0011 0.0012 0.0008 0.0022 0.0009 0.0023 0.0003 0.0004 0.0009 0.0006 0.0006
Nucleolus 0.0004 0.0002 0.0003 0.0001 0.001 0.0001 0.0009 0.0003 0.0005 0.0003 0.0003 0.0001 0.0002 0.0002 0.0009 0.0002 0.0003 0.0001
Nucleus 0.0008 0.0006 0.0011 0.0001 0.0171 0.0012 0.0052 0.0012 0.0009 0.0015 0.0007 0.0012 0.0037 0.0007 0.0012 0.0006 0.0004 0.0017
Peroxisomes 0.017 0.0002 0.0102 0.0139 0.0043 0.0033 0.0382 0.0009 0.0098 0.009 0.0184 0.0056 0.0059 0.0003 0.0033 0.0039 0.0077 0.0008
Punctate Nuclear 0.0033 0.0006 0.0015 0.0001 0.006 0.0009 0.0052 0.0016 0.0018 0.0011 0.0013 0.0011 0.0009 0.0011 0.0045 0.0013 0.0257 0.0007
Vacuole 0.0006 0.0003 0.0003 0.0001 0.0004 0.0005 0.0078 0.0003 0.0013 0.0024 0.0052 0.0003 0.0019 0.0004 0.0013 0.001 0.0003 0.0006
Vacuole Periphery 0.0004 0.0001 0.0002 0 0.0002 0.0001 0.003 0.0001 0.0003 0.0008 0.0024 0.0004 0.0008 0 0.0003 0.0004 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
74 1387 1522 1452 1689 1953 395 1303 1763 3340 1917 2755

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 774.77 711.14 851.38 899.27 633.64 710.35 974.04 864.24 639.56 710.68 876.65 882.70
Standard Deviation 266.37 88.99 124.69 168.78 88.65 84.00 127.83 143.20 106.34 86.11 134.80 158.17
Intensity Change Log 2 — -0.123634 0.136035 0.214986 — 0.164867 0.620318 0.447769 — 0.013337 0.374161 0.324382

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000885 0.000841 0.000261 0.000875 0.000115 0.000733 0.000664 0.002809 0.000147 0.000778 0.000344 0.001790
Bud Neck 0.102857 0.052807 0.005245 0.010754 0.018295 0.041304 0.003796 0.013759 0.021845 0.046081 0.004947 0.012175
Bud Site 0.148195* 0.025947 0.003929 0.038784 0.005236 0.016827 0.004325 0.062943 0.011236 0.020614 0.004010 0.050210
Cell Periphery 0.001328 0.000183 0.000108 0.000374 0.000140 0.000198 0.000222 0.000243 0.000190 0.000192 0.000131 0.000312
Cytoplasm 0.277380* 0.307782* 0.141933* 0.320006* 0.438438* 0.410980* 0.098819 0.432941* 0.431678* 0.368125* 0.133049* 0.373420*
Cytoplasmic Foci 0.107925 0.316780* 0.002569 0.008404 0.256830* 0.264966* 0.009250 0.021990 0.250579* 0.286483* 0.003945 0.014830
Eisosomes 0.000220 0.000225 0.000022 0.000049 0.000207 0.000194 0.000088 0.000048 0.000208 0.000207 0.000036 0.000048
Endoplasmic Reticulum 0.004949 0.001088 0.003890 0.007945 0.000612 0.000721 0.009814 0.001793 0.000794 0.000873 0.005111 0.005035
Endosome 0.031679 0.030867 0.000891 0.004282 0.009130 0.019999 0.002396 0.012264 0.010076 0.024512 0.001201 0.008057
Golgi 0.004474 0.013205 0.000051 0.003563 0.002325 0.006267 0.000073 0.008417 0.002416 0.009148 0.000055 0.005859
Lipid Particles 0.007679 0.010776 0.000653 0.001653 0.012184 0.008096 0.002667 0.001123 0.011995 0.009209 0.001068 0.001402
Mitochondria 0.004935 0.025652 0.000784 0.003335 0.006081 0.013783 0.000910 0.008434 0.006033 0.018712 0.000810 0.005747
Mitotic Spindle 0.022901 0.007267 0.003071 0.025371 0.001759 0.007266 0.001144 0.059753 0.002646 0.007266 0.002674 0.041633
None 0.004672 0.002518 0.004747 0.009683 0.002420 0.002473 0.008153 0.008270 0.002514 0.002492 0.005448 0.009014
Nuclear Periphery 0.001550 0.000413 0.001833 0.003171 0.000443 0.000636 0.002068 0.001666 0.000490 0.000544 0.001882 0.002459
Nuclear Periphery Foci 0.004222 0.000545 0.000409 0.003237 0.000191 0.000274 0.001281 0.001485 0.000360 0.000386 0.000589 0.002409
Nucleolus 0.004547 0.002133 0.000641 0.001472 0.000914 0.001678 0.001183 0.001201 0.001067 0.001867 0.000753 0.001343
Nucleus 0.175180* 0.089332 0.812945* 0.464752* 0.201343* 0.127711 0.797117* 0.270485* 0.200245* 0.111773 0.809683* 0.372872*
Peroxisomes 0.011387 0.042251 0.000166 0.000881 0.008720 0.019572 0.000412 0.004176 0.008832 0.028990 0.000216 0.002439
Vacuole 0.071231 0.066807 0.015703 0.088411 0.033577 0.054710 0.055388 0.079752 0.035157 0.059733 0.023881 0.084316
Vacuole Periphery 0.011804 0.002579 0.000151 0.002999 0.001040 0.001612 0.000231 0.006447 0.001492 0.002014 0.000168 0.004630

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.29 2.13 0.18 -0.30 -7.87 -2.61 -6.41 -2.48 -1.11 -1.06 -4.38 -8.43 -4.02 -1.15 -2.79
Bud Neck 2.15 4.32 3.96 13.45 -7.02 -9.28 6.81 3.36 12.31 -4.47 -10.48 9.52 4.86 18.08 -8.80
Bud Site 4.79 5.67 4.86 0.64 -7.42 -6.77 0.81 -8.70 -4.54 -8.73 -5.09 4.25 -6.88 -3.16 -11.44
Cell Periphery 3.24 3.45 2.98 -3.69 -7.66 -3.11 -4.12 -2.32 1.07 1.84 -0.16 3.24 -1.81 -1.95 -6.76
Cytoplasm -0.91 4.31 1.05 7.10 -14.22 3.10 36.14 13.85 10.98 -20.28 8.05 41.48 20.46 14.29 -23.40
Cytoplasmic Foci -9.99 5.50 5.21 48.23 -6.18 -1.01 44.67 42.59 46.39 -5.89 -5.25 46.16 44.12 66.72 -10.68
Eisosomes -0.10 5.45 4.41 14.89 -12.90 0.80 7.00 9.62 13.77 4.08 0.08 12.39 10.47 20.34 -9.83
Endoplasmic Reticulum 3.02 -0.23 -3.56 -18.33 -9.34 -0.73 -13.29 -16.46 -14.35 9.20 -0.50 -20.77 -24.11 -22.87 -0.79
Endosome 0.14 4.82 4.43 14.02 -7.93 -8.43 8.61 2.70 10.43 -4.86 -11.44 11.76 6.31 17.97 -8.94
Golgi -4.52 2.92 1.96 9.06 -1.84 -5.76 10.00 -0.09 3.44 -2.60 -10.33 10.51 1.06 8.90 -3.19
Lipid Particles -1.60 4.86 4.05 7.92 -8.39 4.04 10.79 11.97 11.24 2.87 2.77 13.20 12.26 12.88 -6.92
Mitochondria -7.89 2.94 1.99 10.31 -4.00 -4.98 6.37 2.41 6.71 -2.91 -8.73 6.72 3.85 12.40 -4.24
Mitotic Spindle 1.45 1.85 -1.12 -7.31 -8.71 -3.83 -1.25 -11.51 -9.95 -10.44 -3.80 -0.52 -14.00 -12.22 -13.84
None 2.07 0.29 -2.52 -5.60 -3.92 -0.27 -2.35 -8.07 -7.32 -1.21 -0.15 -4.52 -8.87 -8.81 -4.82
Nuclear Periphery 1.56 -1.14 -4.73 -21.06 -11.85 -0.74 -15.45 -19.23 -11.77 -5.80 -0.35 -23.62 -27.43 -20.05 -13.95
Nuclear Periphery Foci 2.31 2.28 -0.68 -8.32 -8.37 -2.15 -7.00 -6.85 -6.70 -3.38 -0.33 -5.06 -10.82 -10.81 -9.51
Nucleolus 1.96 3.53 0.72 -1.95 -4.87 -4.06 -0.38 -4.19 -2.63 -4.02 -4.69 2.94 -5.67 -3.35 -6.53
Nucleus 3.23 -22.17 -10.16 -43.80 34.44 12.17 -41.39 -14.73 -23.89 28.17 16.26 -85.07 -28.30 -46.77 49.97
Peroxisomes -7.17 3.14 3.03 16.49 -2.86 -6.73 9.77 6.79 12.53 -3.20 -12.72 10.29 8.71 20.79 -3.99
Vacuole 0.55 4.07 -6.58 -14.37 -22.00 -8.96 -7.96 -23.61 -20.24 -9.13 -11.45 -2.36 -30.48 -24.92 -27.00
Vacuole Periphery 1.65 2.09 1.91 3.27 -2.38 -2.27 4.14 -1.44 -0.53 -3.08 -1.55 4.39 0.04 1.48 -3.93
Standard name
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation
Localization
Cell Percentages cytoplasm (15%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Fmp48

Fmp48


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fmp48-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available