Standard name
Human Ortholog
Description Penta-EF-hand protein; required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.87 0.9 0.79 0.73 0.67 0.46 0.51 0.33 0.33 0.27 0.38 0.95 0.85 0.86 0.8 0.88 0.82 0.79 0.71 0.81 0.86 0.85 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0.08 0 0.21 0.48 0.4 0.56 0.54 0.73 0.52 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0.08 0.08 0.1 0.13 0.16 0.22 0.27 0.2 0.23 0 0 0 0 0 0 0.05 0.1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.19 0.06 0.18 0.21 0.18 0.11 0.09 0.11 0.07 0.05 0.12 0.06 0.23 0.2 0.26 0.18 0.16 0.06 0.11 0.05 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 0 5 5
Bud 2 0 1 0 0 1 4 2 3 2 2 6 0 0 0 0 0 1 0 0 0 0 5 6
Bud Neck 0 0 1 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0 1 1 1 0 1 2
Bud Site 0 0 0 0 0 1 1 3 2 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 1 0 0 1 1 0 0 2 0 6 7 3 0 0 0 0 0 0
Cytoplasm 102 77 123 61 123 100 112 125 78 65 59 75 206 330 305 212 210 182 99 73 133 135 268 258
Endoplasmic Reticulum 1 1 4 0 0 0 0 0 0 1 1 0 2 1 0 5 3 8 0 0 1 0 2 3
Endosome 0 0 0 0 4 1 0 0 0 0 0 0 0 11 4 1 1 3 1 1 1 0 2 3
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 2 0 1 0 0 3 0
Mitochondria 10 2 2 6 2 31 119 98 132 106 159 104 1 2 0 1 1 4 2 2 4 2 6 6
Nucleus 1 1 5 6 14 15 33 40 52 53 43 45 1 0 4 5 6 3 6 10 5 3 2 0
Nuclear Periphery 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 2
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 1 1 1 1 0 0 1 0 0 0 0 0 0 0 0 0 2 4 6
Vac/Vac Membrane 4 17 8 14 36 27 28 22 25 13 11 24 12 90 71 68 44 36 7 10 8 7 7 6
Unique Cell Count 110 89 136 77 169 150 246 244 236 195 218 199 216 388 355 265 239 223 127 103 164 158 317 313
Labelled Cell Count 122 98 144 87 184 178 300 293 293 243 276 257 222 437 386 299 274 244 127 103 164 158 317 313


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 6.3 5.5 5.5 5.5 4.6 4.1 4.3 3.9 3.8 3.6 4.0 5.6 6.1 6.1 7.3 7.3 6.9 5.5 6.0 6.3
Std Deviation (1e-4) 0.4 1.1 1.0 1.7 1.5 1.8 1.3 1.3 1.1 0.6 0.7 0.9 1.0 1.5 1.6 1.5 1.2 1.4 1.1 1.9 1.5
Intensity Change (Log2) – – – 0.0 -0.02 -0.26 -0.43 -0.35 -0.51 -0.53 -0.62 -0.48 0.02 0.15 0.15 0.41 0.41 0.32 -0.01 0.11 0.18

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.3 -3.9 -4.8 -8.6 -7.7 -10.7 -10.3 -11.6 -9.6 1.8 -1.6 -1.3 -2.7 -0.8 -2.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 1.7 2.1 3.0 3.7 4.7 5.5 4.3 4.8 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.8 3.8 3.1 1.8 1.1 1.5 0.3 -0.3 1.9 -0.1 4.5 3.8 4.8 3.4 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0926 0.3387 0.191 -0.1697 -0.1231 0.2524 0.7888 1.2035 1.0404 1.747 1.5249 0.9543 -0.0666 0.1287 -0.1031 0.3012 -0.0743 0.021
Actin 0.0191 0.0008 0.0062 0.0019 0.0078 0.0043 0.0331 0.0014 0.0282 0.0599 0.0086 0.0088 0.0252 0.0004 0.0254 0.0008 0.0026 0.0004
Bud 0.0047 0.0072 0.0044 0.0007 0.0002 0.0034 0.0023 0.0003 0.0039 0.0032 0.0005 0.0004 0.0003 0.0032 0.0002 0.0003 0.0001 0.0001
Bud Neck 0.0035 0.0009 0.0008 0.0015 0.0005 0.0063 0.004 0.0003 0.0109 0.0015 0.0136 0.0243 0.0016 0.0003 0.0011 0.001 0.0006 0.0035
Bud Periphery 0.0014 0.0016 0.0051 0.0008 0.0002 0.003 0.0028 0.0002 0.0026 0.007 0.0008 0.0006 0.0005 0.0011 0.0002 0.0005 0.0001 0
Bud Site 0.0074 0.0105 0.0108 0.0011 0.0002 0.0008 0.0162 0.004 0.0046 0.02 0.0073 0.0014 0.0034 0.0078 0.0006 0.0011 0.0004 0.0001
Cell Periphery 0.0004 0.0002 0.0002 0 0 0.0001 0.0004 0.0001 0.0003 0.0012 0.0003 0.0001 0.0004 0.0002 0.0001 0.0004 0 0
Cytoplasm 0.3612 0.4296 0.4239 0.5128 0.4497 0.4782 0.3429 0.4648 0.4084 0.3358 0.4884 0.5214 0.5818 0.7063 0.554 0.5822 0.5963 0.6196
Cytoplasmic Foci 0.0356 0.0103 0.0135 0.0215 0.0418 0.0179 0.0305 0.0096 0.0112 0.0347 0.0092 0.0112 0.0205 0.0067 0.0145 0.0096 0.0245 0.0143
Eisosomes 0.0005 0.0001 0.0002 0 0.0001 0.0001 0.0002 0.0001 0.0012 0.0006 0.0002 0.0001 0.0005 0 0.0001 0.0004 0 0
Endoplasmic Reticulum 0.0063 0.0027 0.0042 0.0048 0.006 0.0029 0.0061 0.0038 0.0054 0.0052 0.0076 0.0027 0.0121 0.0046 0.0075 0.0031 0.0059 0.0048
Endosome 0.0229 0.0036 0.0108 0.0151 0.0081 0.0065 0.0202 0.0053 0.0098 0.025 0.0113 0.0043 0.0196 0.0032 0.0112 0.0096 0.0137 0.0151
Golgi 0.0057 0.0003 0.0026 0.0022 0.0011 0.0027 0.0072 0.0003 0.0028 0.0099 0.0033 0.0013 0.0036 0.0001 0.0033 0.0006 0.0028 0.0009
Lipid Particles 0.0065 0.0002 0.0061 0.0028 0.0013 0.0018 0.0124 0.0004 0.0098 0.0327 0.0027 0.0064 0.0055 0.0001 0.0032 0.0028 0.0119 0.002
Mitochondria 0.0058 0.0007 0.0134 0.0187 0.0013 0.0059 0.0021 0.0008 0.0032 0.0066 0.0022 0.0024 0.0043 0.0005 0.0011 0.005 0.0011 0.0005
None 0.4511 0.4371 0.4228 0.3192 0.3743 0.3857 0.4344 0.4431 0.3952 0.4023 0.3638 0.3742 0.2318 0.224 0.2176 0.2883 0.2718 0.2486
Nuclear Periphery 0.0088 0.0033 0.0078 0.0054 0.0048 0.0041 0.0073 0.0036 0.0178 0.0036 0.0242 0.0025 0.0135 0.0038 0.0101 0.0055 0.0075 0.0048
Nucleolus 0.0031 0.0009 0.0012 0.0006 0.0004 0.0009 0.0008 0.0005 0.0013 0.0011 0.0014 0.0004 0.0019 0.0005 0.0005 0.0155 0.0003 0.0009
Nucleus 0.0276 0.0748 0.0453 0.0504 0.0514 0.0516 0.0359 0.0508 0.049 0.0082 0.0443 0.0237 0.056 0.0315 0.1374 0.059 0.025 0.069
Peroxisomes 0.0145 0.0004 0.0054 0.0171 0.0123 0.003 0.0259 0.0003 0.0167 0.0308 0.001 0.0044 0.0057 0.0001 0.0017 0.0007 0.0239 0.0004
Punctate Nuclear 0.0094 0.013 0.0117 0.0197 0.0371 0.0196 0.0127 0.0087 0.0139 0.0063 0.005 0.0079 0.0056 0.0035 0.0077 0.0073 0.0092 0.013
Vacuole 0.0033 0.0018 0.0028 0.0031 0.0012 0.001 0.0019 0.0013 0.003 0.0036 0.0031 0.0012 0.0046 0.0018 0.0022 0.005 0.0017 0.0017
Vacuole Periphery 0.0012 0.0002 0.0006 0.0005 0.0003 0.0003 0.0006 0.0002 0.0008 0.0009 0.0013 0.0002 0.0017 0.0002 0.0005 0.0014 0.0004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.5679 10.764 4.3567 10.572 8.5155 12.0345 11.8729 9.349 10.9802 9.9517
Translational Efficiency 2.501 1.3255 2.6446 1.0728 1.9263 1.4944 1.2295 1.7444 1.3451 1.6432

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1399 2390 1633 123 1899 2140 1184 1090 3298 4530 2817 1213

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 728.00 852.73 851.52 845.35 717.27 807.01 939.78 846.01 721.82 831.13 888.62 845.94
Standard Deviation 84.15 100.69 106.08 129.23 84.84 97.96 109.75 112.83 84.71 102.00 116.12 114.60
Intensity Change Log 2 — 0.228151 0.226102 0.215610 — 0.170070 0.389807 0.238158 — 0.199618 0.309668 0.226845

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000082 0.000147 0.000313 0.000373 0.000063 0.000217 0.000397 0.000222 0.000071 0.000180 0.000348 0.000237
Bud Neck 0.008753 0.011090 0.003855 0.003174 0.005550 0.014034 0.004455 0.002782 0.006909 0.012481 0.004107 0.002821
Bud Site 0.003179 0.006306 0.005757 0.007015 0.002587 0.011661 0.004641 0.005242 0.002838 0.008836 0.005288 0.005422
Cell Periphery 0.000144 0.000054 0.000096 0.000312 0.000090 0.000103 0.000170 0.000122 0.000113 0.000077 0.000127 0.000141
Cytoplasm 0.497289 0.545622 0.343586 0.320186 0.519716 0.505644 0.291663 0.452984 0.510203 0.526736 0.321762 0.439518
Cytoplasmic Foci 0.201691 0.055044 0.005447 0.035794 0.093633 0.083580 0.010295 0.016360 0.139471 0.068525 0.007484 0.018330
Eisosomes 0.000128 0.000027 0.000020 0.000112 0.000056 0.000049 0.000048 0.000030 0.000087 0.000037 0.000032 0.000038
Endoplasmic Reticulum 0.000802 0.000961 0.002814 0.005494 0.001061 0.000889 0.003757 0.002182 0.000951 0.000927 0.003210 0.002518
Endosome 0.007219 0.002536 0.000896 0.006249 0.002688 0.005907 0.002672 0.002152 0.004610 0.004129 0.001642 0.002567
Golgi 0.000937 0.000596 0.000053 0.000146 0.000727 0.001174 0.000187 0.000090 0.000816 0.000869 0.000109 0.000095
Lipid Particles 0.007516 0.001044 0.000575 0.005142 0.002402 0.002314 0.001838 0.001040 0.004571 0.001644 0.001106 0.001456
Mitochondria 0.003703 0.001274 0.000843 0.000431 0.001593 0.004011 0.000853 0.001525 0.002488 0.002567 0.000847 0.001414
Mitotic Spindle 0.000425 0.006285 0.002250 0.000500 0.002242 0.012124 0.002075 0.009325 0.001471 0.009044 0.002177 0.008430
None 0.002833 0.002589 0.004957 0.004117 0.003789 0.003072 0.006375 0.004421 0.003384 0.002817 0.005553 0.004390
Nuclear Periphery 0.000525 0.000610 0.001358 0.002026 0.000528 0.000907 0.001812 0.001099 0.000527 0.000750 0.001549 0.001193
Nuclear Periphery Foci 0.000509 0.000441 0.000714 0.003220 0.000308 0.000750 0.001305 0.001280 0.000393 0.000587 0.000962 0.001476
Nucleolus 0.001059 0.000792 0.000237 0.001189 0.000636 0.001029 0.000620 0.000337 0.000816 0.000904 0.000398 0.000424
Nucleus 0.220536 0.326508 0.602304 0.458445 0.336748 0.310279 0.621374 0.460956 0.287451 0.318841 0.610319 0.460701
Peroxisomes 0.004021 0.001831 0.000288 0.000609 0.001436 0.003193 0.000462 0.000351 0.002533 0.002474 0.000361 0.000378
Vacuole 0.037957 0.035593 0.023541 0.144438 0.023843 0.038092 0.044798 0.037314 0.029830 0.036773 0.032475 0.048176
Vacuole Periphery 0.000694 0.000651 0.000099 0.001032 0.000302 0.000971 0.000202 0.000186 0.000468 0.000802 0.000142 0.000272

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.02 -5.18 -6.93 -5.84 -2.26 -4.89 -3.90 -6.54 -2.10 1.81 -6.39 -6.10 -7.72 -4.19 1.51
Bud Neck -1.55 3.72 3.88 4.91 0.47 -6.39 0.54 2.35 8.38 1.38 -5.63 2.83 4.68 9.48 1.77
Bud Site -3.08 -1.97 -1.36 0.03 -0.23 -6.34 -2.09 -2.56 3.39 -0.80 -7.01 -2.90 -2.68 2.37 -0.45
Cell Periphery 12.53 4.26 -4.20 -7.45 -5.51 -2.01 -8.29 -3.39 -2.35 2.33 7.84 -3.21 -2.86 -6.10 -1.20
Cytoplasm -4.85 19.67 9.56 11.56 1.72 1.95 30.61 11.60 10.02 -14.38 -2.05 34.85 13.38 15.04 -11.01
Cytoplasmic Foci 25.61 36.70 24.67 5.40 -4.78 2.48 27.14 24.12 22.70 -5.15 20.43 43.75 38.33 25.24 -8.42
Eisosomes 14.22 13.96 0.75 -8.09 -8.02 1.51 -1.12 3.76 3.76 6.86 12.11 10.66 8.89 -3.70 -1.80
Endoplasmic Reticulum -2.09 -13.74 -6.80 -6.58 -3.50 0.37 -10.80 -5.69 -11.92 7.62 -0.17 -14.27 -9.68 -13.43 3.92
Endosome 7.95 11.19 1.62 -3.80 -5.78 -5.16 0.60 1.50 6.60 1.06 1.37 9.37 5.72 4.52 -3.33
Golgi 2.30 8.18 7.20 3.83 -4.52 -1.18 2.11 2.33 4.53 0.72 -0.17 4.39 4.39 6.01 -0.27
Lipid Particles 10.38 10.92 2.94 -3.31 -3.65 0.27 1.57 6.00 5.51 3.00 9.79 11.03 9.72 1.19 -1.13
Mitochondria 2.48 2.94 3.33 4.97 1.89 -2.87 1.54 0.25 2.70 -0.83 -0.12 3.26 1.46 1.77 -0.75
Mitotic Spindle -4.66 -4.32 -2.23 2.47 0.32 -4.90 -0.48 -3.59 0.30 -3.45 -6.36 -2.10 -4.06 -0.53 -3.48
None 0.89 -4.52 -1.15 -1.46 0.82 1.17 -2.12 -1.49 -3.31 0.74 1.61 -3.73 -2.58 -4.37 0.47
Nuclear Periphery -2.53 -15.33 -6.57 -6.17 -2.77 -1.39 -20.69 -14.31 -2.63 7.80 -1.91 -25.52 -16.17 -6.56 3.46
Nuclear Periphery Foci 0.19 -5.94 -5.13 -5.14 -4.29 -1.73 -7.48 -7.89 -2.72 1.02 -1.80 -9.82 -8.88 -5.95 -2.33
Nucleolus 1.36 5.08 0.52 -0.79 -4.46 -2.19 0.71 2.53 4.56 2.51 -0.61 4.69 3.95 4.67 -0.77
Nucleus -15.14 -46.21 -8.44 -4.29 6.33 3.25 -31.18 -14.21 -16.85 13.26 -6.23 -51.60 -20.95 -17.51 14.94
Peroxisomes 4.07 8.15 7.72 4.75 -1.78 -3.81 3.10 4.95 7.21 0.85 0.22 8.60 9.08 8.61 0.39
Vacuole -0.68 0.07 -8.40 -8.33 -8.39 -7.71 -13.14 -10.62 -6.15 1.98 -6.45 -10.46 -11.84 -9.37 -5.71
Vacuole Periphery 0.27 4.60 0.08 -0.08 -1.54 -4.24 2.29 1.96 5.11 0.03 -2.25 5.34 3.02 4.08 -1.43
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Penta-EF-hand protein; required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
Localization
Cell Percentages cytoplasm (56%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pef1

Pef1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pef1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available