Standard name
Human Ortholog
Description Formylglycinamidine-ribonucleotide (FGAM)-synthetase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.06 0 0.06 0 0.06 – 0.16 0.14 0 0.05 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.98 0.99 0.97 0.97 0.9 0.86 0.89 – 0.85 0.84 1.0 0.98 0.98 0.82 0.8 0.68 0.98 0.96 0.97 0.96 0.94 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0.07 0.09 0.07 – 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 1 4 8 9 – 7 5 0 0 0 0 0 0 1 1 0 0 1 1
Bud 1 4 1 0 0 0 3 1 1 – 0 0 0 1 1 0 0 0 1 6 0 0 6 4
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 – – – – – –
Cell Periphery 6 19 3 4 18 7 24 13 18 – 32 39 3 19 12 4 2 1 0 0 0 1 0 0
Cytoplasm 333 667 336 235 305 338 336 257 288 – 175 232 429 401 428 149 49 68 326 650 330 200 422 433
Endoplasmic Reticulum 0 2 4 2 3 6 28 26 23 – 8 19 1 0 7 6 2 1 0 1 2 0 5 6
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 5 5 3 0 0 0 0 0 0
Golgi 1 1 1 0 0 0 0 1 1 – 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2 0 2 8 – 6 11 0 0 0 3 0 1 0 1 0 0 0 0
Nucleus 0 0 0 0 0 0 0 2 1 – 0 1 0 0 0 8 1 3 0 3 1 1 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 1 0 1 0 6 2 10 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 3 0 4 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 3 0 5 2 10 0 0 0 0 0 0
Vac/Vac Membrane 1 1 1 0 0 0 0 2 2 – 4 2 0 1 5 10 2 7 2 4 2 1 3 3
Unique Cell Count 335 670 343 237 314 348 375 299 325 205 276 429 408 436 182 61 100 334 678 341 209 449 460
Labelled Cell Count 342 694 346 241 328 354 395 312 351 232 310 433 426 453 199 67 111 334 678 341 209 449 460


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 141.8 111.9 67.4 67.5 59.4 49.0 43.8 36.3 34.3 – 30.4 29.0 112.8 108.9 95.2 84.8 94.2 78.6 101.4 105.7 100.8
Std Deviation (1e-4) 32.2 26.7 15.8 17.3 14.2 12.0 11.6 10.0 8.7 – 8.5 8.1 26.3 30.0 27.1 51.8 52.2 60.3 25.4 29.1 26.6
Intensity Change (Log2) – – – 0.0 -0.18 -0.46 -0.62 -0.89 -0.97 – -1.15 -1.21 0.74 0.69 0.5 0.33 0.48 0.22 0.59 0.65 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 3.5 0 3.9 2.8 3.5 – 6.8 6.5 0 3.1 1.9 0 0 0
Cytoplasm 1.1 -0.7 -0.7 -4.6 -5.7 -4.9 – -5.7 -6.3 3.0 0.3 0.2 -6.6 -6.0 -9.3
Endoplasmic Reticulum 0 0 0 4.1 4.5 3.9 – 0 3.7 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 132.5006 171.2253 160.5215 148.0237 148.0328 169.7061 114.3406 150.5748 136.3983 121.1048 115.0021 136.6255 122.9623 160.6472 137.62 131.3264 143.6217 96.7845
Actin 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0009 0.0001 0.0008 0.0172 0.0004 0.0004 0.0002 0.0012 0.0068 0.0001 0.0001 0.0265
Bud 0.0002 0.0001 0.0002 0.0002 0.0002 0.0002 0.0004 0.0001 0.0001 0.0017 0.0002 0.0001 0.0002 0.0001 0.0009 0.0012 0 0.0009
Bud Neck 0.0005 0.0006 0.0007 0.0009 0.0015 0.0078 0.0003 0.0001 0.0004 0.0006 0.0003 0.0006 0.0002 0.0001 0.0002 0.0002 0.0002 0.0014
Bud Periphery 0.0002 0 0.0001 0.0001 0 0.0002 0.0003 0 0 0.0019 0.0002 0 0.0003 0.0001 0.0007 0.0012 0 0.0009
Bud Site 0.0001 0.0001 0.0001 0 0 0 0.0002 0 0.0001 0.0007 0.0002 0 0.0001 0.0034 0.0011 0.0001 0 0.0034
Cell Periphery 0.0023 0.0051 0.0022 0.0031 0.0012 0.007 0.0017 0.0021 0.0005 0.0011 0.0021 0.0003 0.0004 0.0001 0.0005 0.0016 0.0001 0.0002
Cytoplasm 0.939 0.9808 0.9732 0.9556 0.9643 0.9571 0.9355 0.9887 0.9806 0.9483 0.8929 0.9616 0.9676 0.9924 0.934 0.9246 0.9975 0.8488
Cytoplasmic Foci 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0021 0.0001 0.0027 0.0034 0.0018 0.0074 0.0083 0.0002 0.0129 0.0089 0.0002 0.0124
Eisosomes 0 0 0 0 0 0 0.0002 0 0 0 0.0003 0.0001 0 0 0.0001 0.0001 0 0.0002
Endoplasmic Reticulum 0.0027 0.0017 0.0033 0.0008 0.0007 0.0018 0.0024 0.0027 0.0015 0.0005 0.0006 0.0126 0.0008 0.0003 0.0005 0.0012 0.0004 0.001
Endosome 0.0002 0.0001 0.0001 0.0001 0.0002 0.0002 0.0043 0.0001 0.0034 0.0009 0.0215 0.0006 0.0003 0.0003 0.0025 0.0005 0.0001 0.0294
Golgi 0 0 0 0 0 0 0.0005 0 0.0003 0.002 0.012 0.0002 0.0001 0.0001 0.0097 0.0001 0 0.0357
Lipid Particles 0.0001 0.0003 0.0001 0.0001 0.0001 0.0002 0.0041 0.0005 0.0004 0.0015 0.009 0.0014 0.0022 0.0001 0.0022 0.0028 0.0001 0.0091
Mitochondria 0.0005 0 0.0001 0 0 0.0001 0.0063 0 0.0005 0.0008 0.0083 0.0002 0.0004 0.0002 0.0074 0.0034 0 0.0151
None 0.0004 0.0001 0.0001 0.0001 0.0001 0.0002 0.0025 0.0002 0.0029 0.001 0.0036 0.0054 0.0007 0.0001 0.0042 0.0182 0.0001 0.0006
Nuclear Periphery 0.0142 0.0022 0.0047 0.0061 0.0036 0.0066 0.0054 0.0009 0.0018 0.001 0.0037 0.0029 0.0015 0.0003 0.0009 0.0022 0.0003 0.002
Nucleolus 0.0035 0 0.0001 0.0001 0 0.0001 0.0101 0.0001 0.0008 0.0003 0.0193 0.0003 0.0012 0 0.0021 0.0013 0 0.0001
Nucleus 0.0153 0.002 0.0036 0.0048 0.0053 0.0039 0.0086 0.0016 0.0014 0.0027 0.0068 0.0025 0.0022 0.0007 0.003 0.0076 0.0007 0.0021
Peroxisomes 0 0 0 0 0 0 0.0013 0 0.0001 0.0092 0.0003 0.0001 0.0002 0 0.0011 0.0001 0 0.0067
Punctate Nuclear 0 0 0 0 0 0 0.0005 0.0001 0.0004 0.0002 0.0003 0.0014 0.0004 0.0001 0.0006 0.0005 0 0.0007
Vacuole 0.0168 0.0062 0.01 0.0245 0.0196 0.0132 0.0095 0.0025 0.001 0.0046 0.008 0.0017 0.0071 0.0002 0.0051 0.0181 0.0003 0.0018
Vacuole Periphery 0.0037 0.0006 0.0013 0.0034 0.0029 0.001 0.0028 0.0001 0.0002 0.0004 0.008 0.0004 0.0055 0 0.0035 0.0059 0 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 249.3959 212.6624 185.9905 187.6732 153.3425 195.5653 181.0203 166.094 157.7573 178.4623
Translational Efficiency 0.9745 0.9783 0.9073 0.8245 0.7956 0.9874 0.8735 0.6881 0.745 0.6892

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
86 1272 1855 1458 1754 1605 348 1507 1840 2877 2203 2965

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 5114.15 8093.20 7642.18 4115.01 5612.90 7660.55 5393.09 4962.84 5589.59 7851.84 7286.90 4545.93
Standard Deviation 1104.85 1909.74 1356.47 1121.07 1386.52 1843.37 902.09 1083.74 1378.67 1885.29 1533.21 1180.94
Intensity Change Log 2 — 0.662216 0.579490 -0.313599 — 0.448702 -0.057634 -0.177580 — 0.554442 0.281167 -0.240831

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000112 0.000068 0.000040 0.000182 0.000031 0.000059 0.000123 0.000144 0.000035 0.000063 0.000053 0.000162
Bud Neck 0.000710 0.000504 0.000477 0.002524 0.000164 0.000369 0.003128 0.000912 0.000190 0.000428 0.000896 0.001705
Bud Site 0.002625 0.001782 0.000530 0.013269 0.001238 0.001201 0.003470 0.005202 0.001303 0.001458 0.000994 0.009169
Cell Periphery 0.010723 0.006520 0.001235 0.001600 0.002148 0.001886 0.008208 0.006223 0.002549 0.003935 0.002336 0.003949
Cytoplasm 0.834153* 0.893526* 0.867981* 0.819432* 0.940282* 0.918980* 0.776511* 0.891586* 0.935321* 0.907726* 0.853532* 0.856105*
Cytoplasmic Foci 0.021302 0.006791 0.003975 0.007083 0.003431 0.004784 0.013092 0.002318 0.004266 0.005672 0.005415 0.004661
Eisosomes 0.000002 0.000002 0.000001 0.000003 0.000000 0.000001 0.000003 0.000002 0.000001 0.000001 0.000001 0.000002
Endoplasmic Reticulum 0.000098 0.000049 0.000018 0.000164 0.000063 0.000062 0.000038 0.000069 0.000065 0.000056 0.000022 0.000115
Endosome 0.000255 0.000119 0.000037 0.003674 0.000074 0.000091 0.000309 0.000332 0.000083 0.000103 0.000080 0.001976
Golgi 0.019523 0.007253 0.001348 0.004283 0.004500 0.003126 0.005568 0.001418 0.005203 0.004951 0.002015 0.002827
Lipid Particles 0.005173 0.004447 0.000937 0.004382 0.002309 0.001670 0.012943 0.005407 0.002443 0.002898 0.002834 0.004903
Mitochondria 0.000069 0.000093 0.000100 0.000358 0.000089 0.000095 0.000065 0.000592 0.000088 0.000094 0.000094 0.000477
Mitotic Spindle 0.000630 0.000629 0.000325 0.003095 0.000159 0.000481 0.000820 0.000165 0.000181 0.000546 0.000403 0.001606
None 0.002350 0.007557 0.009009 0.001739 0.006650 0.006410 0.002880 0.001742 0.006449 0.006917 0.008041 0.001741
Nuclear Periphery 0.000358 0.000331 0.000232 0.000120 0.000094 0.000123 0.000558 0.000092 0.000106 0.000215 0.000284 0.000106
Nuclear Periphery Foci 0.004124 0.003490 0.001314 0.001773 0.002015 0.001808 0.005866 0.001500 0.002114 0.002552 0.002033 0.001635
Nucleolus 0.000083 0.000087 0.000052 0.000411 0.000047 0.000100 0.000110 0.000086 0.000049 0.000094 0.000061 0.000246
Nucleus 0.060198 0.046894 0.103951 0.038692 0.023356 0.043587 0.076952 0.023253 0.025078 0.045049 0.099686 0.030845
Peroxisomes 0.000009 0.000091 0.000003 0.000397 0.000029 0.000016 0.000017 0.000044 0.000028 0.000049 0.000006 0.000218
Vacuole 0.031405 0.016888 0.007471 0.093511 0.010289 0.013950 0.087667 0.057504 0.011276 0.015249 0.020140 0.075210
Vacuole Periphery 0.006096 0.002879 0.000962 0.003308 0.003031 0.001202 0.001671 0.001409 0.003175 0.001943 0.001074 0.002343

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.60 2.85 -2.33 -4.63 -6.49 -1.78 -9.42 -5.16 -3.37 0.66 -2.89 -7.66 -8.19 -5.26 -5.67
Bud Neck 0.64 -0.49 -9.23 -11.09 -10.51 -3.96 -8.63 -9.49 -5.77 6.76 -4.94 -10.69 -15.63 -12.44 -4.68
Bud Site 0.73 2.24 -6.56 -7.80 -8.92 -0.78 -5.00 -6.70 -6.30 -1.06 -1.41 -0.50 -10.51 -9.89 -10.31
Cell Periphery 1.69 3.97 4.11 7.57 1.69 0.42 -5.83 -6.26 -7.20 1.44 -3.13 0.29 -2.37 1.20 -3.68
Cytoplasm -2.75 -0.57 3.86 17.93 12.46 6.99 18.27 14.53 8.08 -12.25 9.03 23.55 26.14 19.07 1.98
Cytoplasmic Foci 3.23 3.50 2.83 -1.23 -2.31 -3.42 -8.71 -0.40 3.31 8.66 -3.22 -5.80 -2.50 0.25 2.91
Eisosomes -0.16 1.99 -1.11 -0.37 -6.81 -2.29 -8.48 -10.05 -8.04 4.85 -1.56 -7.91 -11.96 -1.91 -5.01
Endoplasmic Reticulum 1.43 2.37 -0.44 -2.54 -3.86 0.02 0.92 -0.21 -0.17 -0.52 0.35 4.27 -1.24 -1.26 -3.09
Endosome 1.77 2.70 -4.12 -4.44 -4.58 -1.69 -5.59 -1.86 -1.62 0.67 -2.00 -2.29 -4.83 -4.71 -4.69
Golgi 3.42 5.14 4.73 5.15 -2.49 2.11 -0.23 6.75 3.83 4.50 0.33 6.79 6.15 6.12 -0.14
Lipid Particles 0.31 2.29 -0.25 -1.29 -8.43 0.79 -7.69 -6.59 -7.76 4.44 -1.53 -2.37 -7.39 -6.69 -5.66
Mitochondria -1.51 -2.73 -1.99 -1.66 -1.49 -0.79 -0.35 -0.97 -0.93 -0.96 -1.02 -2.03 -1.32 -1.24 -1.19
Mitotic Spindle -0.31 0.83 -3.29 -3.18 -3.45 -1.66 -3.06 -1.53 1.41 2.79 -2.37 -3.36 -3.66 -2.72 -3.11
None -9.64 -14.42 3.18 11.42 16.89 0.56 7.21 8.85 8.71 2.79 -0.61 -1.94 9.45 13.99 16.96
Nuclear Periphery 0.02 0.09 2.21 2.38 4.70 -2.33 -7.88 -1.13 1.59 7.71 -3.89 -9.71 -2.79 2.62 8.90
Nuclear Periphery Foci 0.64 3.11 2.57 4.26 -1.73 0.02 -6.29 0.88 0.81 6.83 -2.10 -1.17 0.63 3.42 2.23
Nucleolus -0.89 1.06 -2.96 -1.15 -3.17 -1.55 -7.23 -4.06 0.52 4.32 -1.53 -2.81 -3.58 -1.14 -3.19
Nucleus 1.08 -5.36 0.45 -1.96 15.39 -10.76 -12.47 -3.58 7.28 11.37 -12.23 -29.67 -9.01 2.45 23.11
Peroxisomes -1.09 1.61 -2.27 -1.24 -2.32 0.86 0.20 -1.25 -3.72 -3.11 -0.65 1.78 -2.22 -1.55 -2.63
Vacuole 1.74 2.47 -11.45 -23.94 -25.97 -3.39 -12.35 -18.67 -15.36 5.58 -3.83 -8.57 -30.16 -27.81 -21.49
Vacuole Periphery 2.22 3.62 2.68 1.14 -3.17 3.43 3.04 3.20 -0.17 0.57 2.37 4.11 2.77 0.61 -2.36
Standard name
Human Ortholog
Description Formylglycinamidine-ribonucleotide (FGAM)-synthetase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Ade6

Ade6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ade6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available